CN2452021Y - Micro laser inducing fluorescence detector observation calibrating apparatus - Google Patents
Micro laser inducing fluorescence detector observation calibrating apparatus Download PDFInfo
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- CN2452021Y CN2452021Y CN 00253253 CN00253253U CN2452021Y CN 2452021 Y CN2452021 Y CN 2452021Y CN 00253253 CN00253253 CN 00253253 CN 00253253 U CN00253253 U CN 00253253U CN 2452021 Y CN2452021 Y CN 2452021Y
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Abstract
The utility model relates to a laser inducing fluorescence detection and observation calibrating apparatus, which is composed of a sample poll fine tuning device, an auxiliary light source, an adjustable diaphragm, an eyepiece and a light weakening plate. Laser is assembled on a sample poll via the light weakening plate and a bicolor mirror and through a lens; fluorescence emitted by samples is collected by the lens and is irradiated onto a photomultiplier tube via the bicolor mirror, the light diaphragm and a light filtering piece. The micro laser inducing fluorescence detector observation calibrating apparatus can realize the calibration of light path systems. A laser inducing fluorescence detector is self-assembled through the calibrating apparatus. The system has the advantages of compact structure, less noise, low heterogeneous light level, strong operability of the instrument, convenient calibration, stable performance and high sensitivity. The utility model can be used in the fields of environmental science, natural products, petrochemical industry, biological technique, etc.
Description
The utility model relates to a kind of laser detector, is suitable for the accurate calibration to light path system.
At present, the miniature laser induced fluorescence detects and to be broadly divided into that confocal point detects and two types of vertical detection, these two kinds of detection methods have nothing in common with each other, but the time all need calibration in operation, wherein have a kind of in vertical detection according to reversibility of optical path, place a small light source in the photomultiplier front, observe with an attachment lens on the collecting lens opposite, adjust sample cell and condenser lens position, the three is overlapped, reach the purpose of calibration.These device needs one colour glass filter are efficiently deducted exciting light, but the difficult selection of this optical filter, efficient is too high, do not observe laser spots, efficient is too low, do not have due effect, in addition, this optical filter does not have selectivity to exciting light and fill-in light, the deduction exciting light in, be easy to fill-in light is filtered, be difficult to like this observe sample cell clearly as, the problem that this device exists is: photomultiplier need be removed during observation, this is very inconvenience for valuable, tender and lovely photomultiplier; Laser shines directly into sample cell can produce a large amount of scattered lights, is difficult to observe directly the accurate position of laser spots, and the easy damaged eyes, troublesome poeration.
For solving the deficiency of above measurement mechanism, the purpose of this utility model provides a kind of miniature laser induced fluorescent tester observation calibrating installation, utilizes homemade sample cell micromatic setting, can realize that sample cell can move on two-dimensional directional, the sample fixing-stable, simple in structure, calibrate purpose easily.
Structural design of the present utility model is achieved in that this calibrating installation finely tuned by sample cell on structure, secondary light source, adjustable diaphragm, several parts of eyepiece and weakener are formed (as shown in Figure 1), detailed structure is that the front is a sample cell, sample cell is a sample microcosmic tunable arrangement (as shown in Figure 3), light emitting diode is housed below the sample cell, corresponding with sample cell is lens, above the perpendicular bisector behind lens semiconductor laser is housed, weakener is housed below the laser instrument, below the weakener, the lens perpendicular bisector tilts and is placed with dichroscope, below dichroscope, photodiode is housed, on the lens perpendicular bisector, the dichroscope back is equipped with lens equally, with the front lens on a horizontal line, on this lens back perpendicular bisector diaphragm is housed, sight glass is equipped with on top, diaphragm back, vertically downward, in the sight glass back, on the perpendicular bisector of lens optical filter is housed, the optical filter back is a photomultiplier, wherein sight glass is made up of three eyeglasses, be eyepiece above, the centre is object lens, is 45 ° of level crossings below; Sample cell is a mechanical hook-up, below for having slideway base, be cross sliding clock above, promptly be specimen holder, in the cross sliding clock front vertical slipper be housed, the back of cross sliding clock is equipped with and is moved horizontally bar and horizontal fixed plate, moving horizontally bar connects with specimen holder by screw thread, but the specimen holder horizontal vertical is moved, vertical fixing plate (as shown in Figure 3) is housed in the slideway side, whole device is fixed on the horizontal base.
Advantage of the present utility model:
The utility model provides a kind of miniature laser induced fluorescent tester observation calibrating installation that is used for, and utilizes this finder can be directly used in close adjustment to light path system.One confocal some laser induced fluorescence detector system architecture compactness of self assembly, volume is 13 * 37 * 9cm only, weighs 5 kilograms, small and exquisite sample cell, suitable sample cell technique for fixing, effective calibrating installation, adjustable diaphragm adds that stable light source makes this noise of instrument little, stray light level is low, instrument workable, calibration is convenient, and stable performance is highly sensitive.Can be widely used in environmental science, natural products, petrochemical complex and biotechnology field.
Detailed structure of the present utility model is provided by following examples and accompanying drawing.
Fig. 1 is a miniature laser induced fluorescent tester apparatus structure schematic diagram.
Fig. 2 is miniature laser induced fluorescent tester observation calibrating installation sight glass structural drawing.
Fig. 3 is miniature laser induced fluorescent tester observation calibrating installation sample cell fine tuning structure figure.
Fig. 4 is miniature laser induced fluorescent tester observation calibrating installation sample cell fine setting perspective view.
Detailed structure as shown in drawings, 1 is sample cell, for depositing the place of sample, below 2 be light emitting diode, facing to 3 of sample cell 1,8 is lens, 4 is semiconductor laser above the lens perpendicular bisector, below 5 be weakener, it 6 is dichroscope that perpendicular bisector tilts, with laser instrument corresponding 7 be photodiode, 10 is optical filter on the lens perpendicular bisector, 11 is diaphragm, and top 9 is sight glass, and last 12 is photomultiplier, wherein sight glass 13 is an eyepiece, 14 is object lens, and 15 is 45 ° of level crossings, and sample pool structure 16 is for moving horizontally bar, 17 is the horizontal fixed plate, 18 is cross sliding clock, promptly is specimen holder, and 19 is the vertical fixing plate, 20 is vertical slipper, and 21 is slideway base.
Fig. 1 is the optical system configuration composition of miniature laser induced fluorescent tester, during calibration, at first open secondary light source 2, sight glass 9 is screwed on the observation station between diaphragm and the optical filter, just be arranged in light path, on Z-direction fine setting sample cell 1 make it to present clearly at diaphragm 11 places as, open weakener 5 then, start laser instrument 4, make laser beam foucing just in time be positioned at the sample cell center at X-direction fine setting sample cell 1, then sight glass 9 is screwed out light path, close weakener 5 and secondary light source 2, screw plastic screw with the moving slider locking position, calibration finishes.
Claims (2)
1. a miniature laser induced fluorescent tester is observed calibrating installation, it is characterized in that: this device is finely tuned by sample cell, secondary light source, adjustable diaphragm, several parts of eyepiece and weakener are formed, detailed structure is that the front is sample cell (1), the sample cell fine setting is a two-dimensional adjustable device, light emitting diode (2) is housed below the sample cell, corresponding with sample cell is lens (3), semiconductor laser (4) is housed above the perpendicular bisector behind lens, weakener is housed below the laser instrument, below the weakener (5), the lens perpendicular bisector tilts and is placed with dichroscope (6), below dichroscope, photodiode (7) is housed, on the lens perpendicular bisector, lens (8) are equipped with in the dichroscope back, with the front lens on a horizontal line, on this lens back perpendicular bisector adjustable diaphragm (11) is housed, sight glass (9) is equipped with on top, diaphragm back, vertically downward, in the sight glass back, optical filter (10) is housed on the perpendicular bisector of lens, the optical filter back is photomultiplier (12), and wherein sight glass is made up of three eyeglasses, is eyepiece (13) above, the centre is object lens (14), is 45 ° of level crossings (15) below.
2. according to the described calibrating installation of claim 1, it is characterized in that: the sample cell fine setting is a mechanical hook-up, below for having slideway base (21), being cross sliding clock (18) above, promptly is specimen holder, and vertical slipper (20) is housed in the cross sliding clock front, the back of cross sliding clock is equipped with and is moved horizontally bar (16) and horizontal fixed plate (17), move horizontally bar and connect with specimen holder, but the specimen holder horizontal vertical is moved, vertical fixing plate (19) is housed in the slideway side by screw thread.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 00253253 CN2452021Y (en) | 2000-11-29 | 2000-11-29 | Micro laser inducing fluorescence detector observation calibrating apparatus |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN 00253253 CN2452021Y (en) | 2000-11-29 | 2000-11-29 | Micro laser inducing fluorescence detector observation calibrating apparatus |
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CN2452021Y true CN2452021Y (en) | 2001-10-03 |
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CN 00253253 Expired - Fee Related CN2452021Y (en) | 2000-11-29 | 2000-11-29 | Micro laser inducing fluorescence detector observation calibrating apparatus |
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Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100403012C (en) * | 2004-07-29 | 2008-07-16 | 中国科学院大连化学物理研究所 | Portable instrument for detecting pesticide residue through fluorescent probe |
CN100458416C (en) * | 2006-02-16 | 2009-02-04 | 陕西师范大学 | Acoustic fluorescence detection device for quantitative analysis and detecting method |
CN102144156A (en) * | 2008-09-10 | 2011-08-03 | 夏普株式会社 | Evaluation device, calibration method, calibration program, and recording medium |
CN102159936A (en) * | 2008-09-18 | 2011-08-17 | 株式会社岛津制作所 | Fluorescent image detector and method for detecting fluorescent image |
CN103017664A (en) * | 2012-11-28 | 2013-04-03 | 中国计量科学研究院 | Method and system for calibrating laser beam analyzer |
CN104483297A (en) * | 2014-12-08 | 2015-04-01 | 上海通微分析技术有限公司 | Visualization calibration laser-induced fluorescence detection device |
CN110686614A (en) * | 2019-10-11 | 2020-01-14 | 西安工业大学 | Detection device and detection method for depth information of subsurface defect of optical element |
CN111650170A (en) * | 2020-07-03 | 2020-09-11 | 中国计量科学研究院 | Measurement standard for calibration of luminescence immunoassay analyzer for bottom or lateral detection |
CN113030501A (en) * | 2021-03-05 | 2021-06-25 | 郑州如飞生物技术有限公司 | Automatic calibration mechanism for fluorescence immunoassay analyzer and automatic calibration method thereof |
CN117554340A (en) * | 2023-10-13 | 2024-02-13 | 北京大学 | Laser-induced fluorescence detection system and detection method |
-
2000
- 2000-11-29 CN CN 00253253 patent/CN2452021Y/en not_active Expired - Fee Related
Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN100403012C (en) * | 2004-07-29 | 2008-07-16 | 中国科学院大连化学物理研究所 | Portable instrument for detecting pesticide residue through fluorescent probe |
CN100458416C (en) * | 2006-02-16 | 2009-02-04 | 陕西师范大学 | Acoustic fluorescence detection device for quantitative analysis and detecting method |
CN102144156A (en) * | 2008-09-10 | 2011-08-03 | 夏普株式会社 | Evaluation device, calibration method, calibration program, and recording medium |
CN102144156B (en) * | 2008-09-10 | 2013-10-02 | 夏普株式会社 | Evaluation device, calibration method, calibration program, and recording medium |
CN102159936A (en) * | 2008-09-18 | 2011-08-17 | 株式会社岛津制作所 | Fluorescent image detector and method for detecting fluorescent image |
CN103017664B (en) * | 2012-11-28 | 2015-04-29 | 中国计量科学研究院 | Method and system for calibrating laser beam analyzer |
CN103017664A (en) * | 2012-11-28 | 2013-04-03 | 中国计量科学研究院 | Method and system for calibrating laser beam analyzer |
CN104483297A (en) * | 2014-12-08 | 2015-04-01 | 上海通微分析技术有限公司 | Visualization calibration laser-induced fluorescence detection device |
CN110686614A (en) * | 2019-10-11 | 2020-01-14 | 西安工业大学 | Detection device and detection method for depth information of subsurface defect of optical element |
CN111650170A (en) * | 2020-07-03 | 2020-09-11 | 中国计量科学研究院 | Measurement standard for calibration of luminescence immunoassay analyzer for bottom or lateral detection |
CN113030501A (en) * | 2021-03-05 | 2021-06-25 | 郑州如飞生物技术有限公司 | Automatic calibration mechanism for fluorescence immunoassay analyzer and automatic calibration method thereof |
CN113030501B (en) * | 2021-03-05 | 2023-07-21 | 郑州如飞生物技术有限公司 | Automatic calibration mechanism for fluorescence immunoassay analyzer and automatic calibration method thereof |
CN117554340A (en) * | 2023-10-13 | 2024-02-13 | 北京大学 | Laser-induced fluorescence detection system and detection method |
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CF01 | Termination of patent right due to non-payment of annual fee |