CN109566912A - A kind of feed and preparation method thereof improving opsariichthys bidens immunity - Google Patents

A kind of feed and preparation method thereof improving opsariichthys bidens immunity Download PDF

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Publication number
CN109566912A
CN109566912A CN201811462908.9A CN201811462908A CN109566912A CN 109566912 A CN109566912 A CN 109566912A CN 201811462908 A CN201811462908 A CN 201811462908A CN 109566912 A CN109566912 A CN 109566912A
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opsariichthys bidens
feed
vegetable protein
immunity
aquiculture
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Inventor
孟繁星
王日昕
黎明
刘嘉欣
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Ningbo University
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Ningbo University
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K40/00—Shaping or working-up of animal feeding-stuffs
    • A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y02A40/818—Alternative feeds for fish, e.g. in aquacultures

Abstract

The present invention provides a kind of feed and preparation method thereof for improving opsariichthys bidens immunity, belongs to technical field of aquaculture, contains vegetable protein used for aquiculture, the Chinese herbal medicine powder comprising trehalulose and isomaltoketose, fish meal, miberal powder, microorganism, vitamin.Wherein the preparation method containing vegetable protein used for aquiculture includes that rapeseed dregs and/or Cottonseed Meal pulverize step；Infra-red radiation step；And solid state fermentation step；Wherein infrared is medium-wave infrared.The preparation method of present invention vegetable protein used for aquiculture can improve the content of soluble dietary fiber, reduce insoluble diedairy fiber mass content；The feed for improving opsariichthys bidens immunity can improve the immunity of opsariichthys bidens, prevent and treat opsariichthys bidens fish disease, improve its cultivation survival rate, it can be reduced the yield of the excreta of opsariichthys bidens again, reduce deposition of the feed in breeding water body, and then the cleannes of breeding water are improved, greatly reduce opsariichthys bidens aquaculture cost.

Description

A kind of feed and preparation method thereof improving opsariichthys bidens immunity
Technical field
The invention belongs to technical field of aquaculture, and in particular to it is a kind of improve opsariichthys bidens immunity feed and its preparation Method.
Background technique
The aquatic products food component part important as the mankind, provides good animal protein source for the health of the mankind. With national economy fast development and people's living standard continuous improvement, people to the consumption of aquatic products started by Pursuit to quality and quality is turned to the satisfaction of quantity, the wild fish on dining table is increasingly liked by people.In order to The requirement for meeting the growing living standard of people adjusts the cultivation structure of aquatic products, the high delicious cultivation of exploitation matter New varieties are imperative.Opsariichthys bidens are a kind of small-sized food meat fresh water warps lived in the limpid water body such as streams, mountain stream extensively Ji fish, hypoxia-resistant capacity is strong, catches arch and is easy, nutritive value is high.Opsariichthys bidens are a kind of small-sized violent predacious fishes, mainly It is food with small fish shrimp and aquatic invertebrate, when body long 30mm starts to switch to carnivorous, and when body long 50mm all switchs to meat Feeding habits.It is serious broken in recent years since interests driving and the deterioration and destruction of ecological environment, the natural resources of opsariichthys bidens are sharply reduced It is broken the bio-diversity of opsariichthys bidens, to protect and rescuing this valuable stock of fish, domestic some areas start artificial breeding Grow and cultivate opsariichthys bidens.But existing opsariichthys bidens feed nutrient be not it is quite reasonable, not only ingredient is single, can not provide Whole nutritional ingredients needed for opsariichthys bidens growth, but also contain various hormones and additive, affect the edible mouth of opsariichthys bidens Taste, while the death rate is higher in the breeding process for opsariichthys bidens, and certain economic loss is caused to raiser.It is artificial in order to improve The economic benefit of cultivation is badly in need of finding a kind of opsariichthys bidens feeding that can be improved opsariichthys bidens immunity, grow and breed conducive to opsariichthys bidens Material.
Summary of the invention
The purpose of the present invention is to provide a kind of contents that can improve soluble dietary fiber, reduce insoluble diedairy fiber matter Measure content vegetable protein used for aquiculture preparation method, be made mixed plant albumen powder in the cellulose containing 5-7% and Oligopeptides.
The technical solution that the present invention is taken to achieve the above object are as follows:
The present invention discloses a kind of preparation method of vegetable protein used for aquiculture, including,
Rapeseed dregs and/or Cottonseed Meal pulverize step；
Infra-red radiation step；And
Solid state fermentation step；
Wherein, infrared is medium-wave infrared.On the one hand infra-red radiation can make the egg of inside microbes by way of heating White matter denaturation avoids later stage fermentation from having the participation of miscellaneous bacteria in the process to death, guarantees the matter of tunning mixed plant albumen Amount, on the other hand can change the structure of the part insoluble diedairy fiber molecule inside rapeseed dregs and/or Cottonseed Meal Ultramicro-powder, Small-molecule substance is released, the content of wherein soluble dietary fiber is improved, then to mix by the fermentation of saccharomycete Containing the cellulose of 5-7% in plant protein powder, the yield of the excreta of opsariichthys bidens is reduced, improves the cleannes of breeding water, The dosage for reducing breeding water inorganic agent greatly reduces opsariichthys bidens aquaculture cost.
Preferably, rapeseed dregs and/or Cottonseed Meal are whole after pulverizing 60% or more has the partial size less than 25 μm. It is preferred that whole 70% or more has the partial size less than 25 μm, more preferably whole 90% or more has the partial size less than 25 μm, The rapeseed dregs of the granularity and/or the specific surface area of Cottonseed Meal and surface can significantly increase, and surface atom, ion populations also increase Add, granule surface activity improves, and plays a role conducive to penetrating for infrared ray, while can improve in rapeseed dregs and/or Cottonseed Meal The physical characteristic of fiber, improve soluble dietary fiber content, reduce insoluble diedairy fiber mass content, additionally fully with Saccharomycete is uniformly mixed, and improves ferment effect.
Preferably, infrared intensity is 5000-6000W/m2, the processing time is 30-50s.
Preferably, the inoculum concentration of saccharomycete is 2.2-2.7%, fermentation temperature is 25-30 DEG C, time 2-3d.
The enzyme material that metabolism of yeasts goes out in preparation process of the present invention is to glucosinolate, phytic acid, tannin and gossypol etc. Substance has degradation, and synthesizes Self substances；Other anti-nutritional factors can also be decomposed by saccharomycete simultaneously, such as phytic acid, list Rather, cellulose etc. can dramatically improve the mouthfeel of rapeseed dregs and/or Cottonseed Meal, improve rapeseed dregs and/or Cottonseed Meal nutritional ingredient Utilization rate；And saccharomycete is acted on using own metabolism, can produce the precursor substance of fragrance matter or fragrance, improves rapeseed dregs And/or the fragrance after Cottonseed Meal detoxification；Furthermore the solubility containing high-content in mixed plant albumen made from the preparation method Fiber has good facilitation to the breeding of microorganism in opsariichthys bidens large intestine, to increase other nutrients and soluble fibre The fermentation in opsariichthys bidens large intestine is tieed up, digestion and absorption of the opsariichthys bidens to feed is improved, reduces the yield of the excreta of opsariichthys bidens；It should Also contain oligopeptides in mixed plant albumen made from preparation method, there is enhancing macrophage phagocytic activity, stimulation lymphocyte It is proliferated and Factor of Macrophage IL-1 β, IL-6, IL-12, TNF-α and NO and lymphocytic emiocytosis IL-2 can be promoted Effect, improve the immunity of opsariichthys bidens, the final survival rate for improving opsariichthys bidens.
The present invention discloses vegetable protein used for aquiculture made from a kind of preparation method of vegetable protein used for aquiculture.
Preferably, containing the cellulose of 2-6% in vegetable protein.
The mixed plant albumen substitution prepared with rapeseed dregs of the present invention and/or Cottonseed Meal through saccharomycetes to make fermentation is part or all of The feedstuff feeding opsariichthys bidens that fish meal obtains, can not only improve the immunity of opsariichthys bidens, and can be improved opsariichthys bidens to feed Digestion and absorption, reduce the yield of the excreta of opsariichthys bidens, improve the cleannes of breeding water, reduce breeding water inorganic agent Dosage, greatly reduce opsariichthys bidens aquaculture cost.
Another object of the present invention is to provide the immunity that one kind can improve opsariichthys bidens, prevent and treat opsariichthys bidens Fish disease improves its cultivation survival rate, and can be reduced the yield of the excreta of opsariichthys bidens, and it is heavy in breeding water body to reduce feed Product, and then the cleannes of breeding water are improved, greatly reduce the raising opsariichthys bidens immunity of the feed of opsariichthys bidens aquaculture cost Feed.
The technical solution that the present invention is taken to achieve the above object are as follows:
Invention additionally discloses a kind of feeds for improving opsariichthys bidens immunity, contain above-mentioned vegetable protein used for aquiculture.
Preferably, the feed for improving opsariichthys bidens immunity also contains the Chinese herbal medicine comprising trehalulose and isomaltoketose Powder, fish meal, miberal powder, vitamin.On the one hand the presence of Chinese herbal medicine powder comprising trehalulose and isomaltoketose can cooperate plant Object albumen and cellulose therein improve digestion and absorption of the opsariichthys bidens to feed, reduce the yield of the excreta of opsariichthys bidens, another The presence of Chinese herbal medicine powder ketone group can be reacted with amino-compound in aspect, and it is even black to ultimately generate brown by complicated process The macromolecular substances melanoid of color improves opsariichthys bidens to the visual perception of fish meal, facilitates opsariichthys bidens to capture food, improve Ma Kou Fish reduces deposition of the feed in breeding water body, improves the cleannes of breeding water to the uptake ratio of feed；In addition, Chinese herbal medicine Effective component in powder can be improved the immunity of opsariichthys bidens, prevents and treats opsariichthys bidens fish disease, improves its cultivation survival rate.
Preferably, the Chinese herbal medicine powder comprising trehalulose and isomaltoketose is obtained by fermentation process.
Invention additionally discloses a kind of preparation methods of feed for improving opsariichthys bidens immunity, by plant egg used for aquiculture Chinese herbal medicine powder white, comprising trehalulose and isomaltoketose, fish meal, miberal powder, vitamin are uniformly mixed, and are granulated to get raising The feed of opsariichthys bidens immunity.
Compared with prior art, the invention has the benefit that
The feed that the present invention improves opsariichthys bidens immunity can improve digestion and absorption of the opsariichthys bidens to feed, reduce opsariichthys bidens Excreta yield, and opsariichthys bidens can be improved to the uptake ratio of feed, reduce deposition of the feed in breeding water body, Jin Erti The cleannes of high breeding water reduce the dosage of breeding water inorganic agent, greatly reduce opsariichthys bidens aquaculture cost；The present invention The feed for improving opsariichthys bidens immunity can be improved the immunity of opsariichthys bidens, prevents and treats opsariichthys bidens fish disease, improves its cultivation Survival rate.
Present invention employs above-mentioned technical proposals to provide a kind of feed and preparation method thereof for improving opsariichthys bidens immunity, more The deficiencies in the prior art, reasonable design, easy operation are mended.
Specific embodiment
In the following, being described further in conjunction with specific embodiments to embodiment of the present invention.
Embodiment 1:
A kind of preparation method of vegetable protein used for aquiculture, including,
1) rapeseed dregs is pulverized, rapeseed dregs is whole after ultramicro grinding 60% or more has the partial size less than 25 μm. It is preferred that whole 70% or more has the partial size less than 25 μm, more preferably whole 90% or more has the partial size less than 25 μm, The rapeseed dregs of the granularity and/or the specific surface area of Cottonseed Meal and surface can significantly increase, and surface atom, ion populations also increase Add, granule surface activity improves, and plays a role conducive to penetrating for infrared ray, while can improve in rapeseed dregs and/or Cottonseed Meal The physical characteristic of fiber, improve soluble dietary fiber content, reduce insoluble diedairy fiber mass content, additionally fully with Saccharomycete is uniformly mixed, and improves ferment effect；
2) it is radiated using medium-wave infrared, infrared intensity 5000W/m2, the processing time is 30s；One side of infra-red radiation Face can make the protein denaturation of inside microbes to death by way of heating, avoid having miscellaneous bacteria during later stage fermentation Participation, guarantee tunning mixed plant albumen quality, on the other hand can change rapeseed dregs and/or Cottonseed Meal Ultramicro-powder The structure of internal part insoluble diedairy fiber molecule releases small-molecule substance, improves containing for wherein soluble dietary fiber Amount then makes the cellulose in mixed plant albumen powder containing 5-7% by the fermentation of saccharomycete, reduces opsariichthys bidens The yield of excreta improves the cleannes of breeding water, reduces the dosage of breeding water inorganic agent, greatly reduces opsariichthys bidens Aquaculture cost；
It 3) is 2.2% inoculation yeast bacterium by inoculum concentration, solid state fermentation 2d under conditions of fermentation temperature is 25 DEG C.
The enzyme material that metabolism of yeasts goes out in the preparation process is to substances such as glucosinolate, phytic acid, tannin and gossypols With degradation, and synthesize Self substances；Other anti-nutritional factors can also be decomposed by saccharomycete simultaneously, such as phytic acid, tannin, fibre Element etc. is tieed up, the mouthfeel of rapeseed dregs and/or Cottonseed Meal can be dramatically improved, improves the benefit of rapeseed dregs and/or Cottonseed Meal nutritional ingredient With rate；And saccharomycete is acted on using own metabolism, can produce the precursor substance of fragrance matter or fragrance, improve rapeseed dregs and/ Or the fragrance after Cottonseed Meal detoxification；Furthermore the solubility in mixed plant albumen made from the preparation method containing high-content is fine Dimension, has good facilitation to the breeding of microorganism in opsariichthys bidens large intestine, to increase other nutrients and Soluble Fiber Fermentation in opsariichthys bidens large intestine improves digestion and absorption of the opsariichthys bidens to feed, reduces the yield of the excreta of opsariichthys bidens；The system Also contain oligopeptides in mixed plant albumen made from Preparation Method, there is enhancing macrophage phagocytic activity, stimulation lymphocyte to increase Grow and can promote Factor of Macrophage IL-1 β, IL-6, IL-12, TNF-α and NO and lymphocytic emiocytosis IL-2 Effect, improves the immunity of opsariichthys bidens, the final survival rate for improving opsariichthys bidens.
A kind of vegetable protein used for aquiculture, the cellulose containing 2-6% in vegetable protein.With the rapeseed dregs and/or cotton The mixed plant albumen that the seed dregs of rice are prepared through saccharomycetes to make fermentation substitutes the feedstuff feeding opsariichthys bidens that part or all of fish meal obtains, not only It can be improved the immunity of opsariichthys bidens, and can be improved digestion and absorption of the opsariichthys bidens to feed, reduce the excreta of opsariichthys bidens Yield, improve the cleannes of breeding water, reduce the dosage of breeding water inorganic agent, greatly reduce opsariichthys bidens cultivation at This.
A kind of feed improving opsariichthys bidens immunity, including following ingredient and parts by weight: vegetable protein 28 used for aquiculture Part, 2 parts of 5 parts of 40 parts of 9 parts of Chinese herbal medicine powder, fish meal, miberal powder, the vitamin comprising trehalulose and isomaltoketose.Include seaweed On the one hand the presence of the Chinese herbal medicine powder of ketose and isomaltoketose can cooperate vegetable protein and cellulose therein, improve Ma Kou Digestion and absorption of the fish to feed, reduces the yield of the excreta of opsariichthys bidens, on the other hand can react with amino-compound, by multiple Miscellaneous process ultimately generates the macromolecular substances melanoid of brown even black, improves opsariichthys bidens to the visual impression of fish meal Official facilitates opsariichthys bidens to capture food, improves opsariichthys bidens to the uptake ratio of feed, reduces deposition of the feed in breeding water body, mention The cleannes of high breeding water；In addition, the effective component in Chinese herbal medicine powder can be improved the immunity of opsariichthys bidens, prevent and treat Opsariichthys bidens fish disease improves its cultivation survival rate.
Wherein, the Chinese herbal medicine powder comprising trehalulose and isomaltoketose is obtained by fermentation process.
A kind of preparation method for the feed improving opsariichthys bidens immunity, by vegetable protein used for aquiculture, includes seaweed ketone The Chinese herbal medicine powder of sugar and isomaltoketose, fish meal, miberal powder are uniformly mixed, and are granulated to get the feed of opsariichthys bidens immunity is improved.
Embodiment 2:
A kind of preparation method of vegetable protein used for aquiculture, including,
1) rapeseed dregs and Cottonseed Meal that weight ratio is 0.8:1 are pulverized, rapeseed dregs and Cottonseed Meal are whole after ultramicro grinding 60% or more of body has the partial size less than 25 μm；
2) it is radiated using medium-wave infrared, infrared intensity 5600W/m2, the processing time is 40s；
It 3) is 2.5% inoculation yeast bacterium by inoculum concentration, solid state fermentation 2.5d under conditions of fermentation temperature is 27 DEG C.
A kind of vegetable protein used for aquiculture, the cellulose containing 2-6% in vegetable protein.
A kind of feed improving opsariichthys bidens immunity, containing above-mentioned 33 parts of vegetable protein used for aquiculture, include seaweed ketone 2.2 parts of 4 parts of 44 parts of 11 parts of Chinese herbal medicine powder, fish meal, miberal powder, the vitamin of sugar and isomaltoketose.
A kind of preparation method for the feed improving opsariichthys bidens immunity, by vegetable protein used for aquiculture, includes seaweed ketone The Chinese herbal medicine powder of sugar and isomaltoketose, fish meal, miberal powder, vitamin are uniformly mixed, and are granulated to get opsariichthys bidens immunity is improved Feed.
Embodiment 3:
A kind of preparation method of vegetable protein used for aquiculture, including,
1) Cottonseed Meal is pulverized, Cottonseed Meal is whole after ultramicro grinding 60% or more has the partial size less than 25 μm；
2) it is radiated using medium-wave infrared, infrared intensity 6000W/m2, the processing time is 50s；
It 3) is 2.7% inoculation yeast bacterium by inoculum concentration, solid state fermentation 3d under conditions of fermentation temperature is 30 DEG C.
A kind of vegetable protein used for aquiculture, the cellulose containing 2-6% in vegetable protein.
A kind of feed improving opsariichthys bidens immunity, containing above-mentioned 36 parts of vegetable protein used for aquiculture, include seaweed ketone 3 parts of 7 parts of 48 parts of 14 parts of Chinese herbal medicine powder, fish meal, miberal powder, the vitamin of sugar and isomaltoketose.
A kind of preparation method for the feed improving opsariichthys bidens immunity, by vegetable protein used for aquiculture, includes seaweed ketone The Chinese herbal medicine powder of sugar and isomaltoketose, fish meal, miberal powder, vitamin are uniformly mixed, and are granulated to get opsariichthys bidens immunity is improved Feed.
Embodiment 4:
The preparation method of Chinese herbal medicine powder comprising trehalulose and isomaltoketose:
1) Enteromorpha successively the separation of Enteromorpha endophyte: is used to antiseptic sea water, 75% ethyl alcohol and sterile water washing.Then, it uses Mortar smashs sample to pieces, then deposit in containing 100mg/mL chloramphenicol PDA culture medium (every liter contain 200 grams of potato extract, 20 grams of glucose, 15 grams of agar and seawater 1L) in, it is cultivated at 28 DEG C to there is single culture, which is transferred to another PDA culture medium is simultaneously stored in 4 DEG C；
2) preparation of the Chinese herbal medicine extract: take by weight 23 parts of Enteromorpha, 12 parts of Radix Astragali, 10 parts of cordate houttuynia, 17 parts of rheum officinale, 11 parts of Schisandra chinensis take each raw material, crush to obtain herbal raw material, and 52 degree of alcohol is sprinkled upon herbal raw material by weight for 1:1 On, 3 times of herbal raw material weight of alkali ion water is added after placement 2h and filters to obtain extracting solution in 85 DEG C of infusion 3h, then exists 10min is sterilized at 100 DEG C；
3) fermentation of extracting solution: will add bacillus subtilis and Enteromorpha endophyte in extracting solution, ferment 6h at 35 DEG C, After fermentation, 10min is sterilized at 100 DEG C, obtains the Chinese herbal medicine powder comprising trehalulose and isomaltoketose.
Wherein, herbal raw material is not limited to mentioned component.
The effective component of herbal raw material is present in cell cytosol, and plant cell wall is by cellulose, hemicellulose, wood The hard and compacts such as quality, pectic substance are at being grouped as, so effective component, which extracts, must overcome cytoplasm and cell wall Dual resistance, and microorganism can generate the ectoenzymes such as cellulase, hemicellulase, pectase during growth metabolism, make Plant cell wall cracking, resistance reduce, extract to the maximum extent to be conducive to Chinese herbal medicine effective ingredients, microorganism growth metabolism Process has very powerful decomposition and inversion ability, can be by the macromolecules degradations that cannot be directly utilized many in active ingredient of Chinese herbs The curative effect of Chinese medicine is improved, in addition, the conversion of microorganism is made to improve the absorption and utilization to effective component for small molecule Structural modification is carried out with the toxic component of Chinese medicine can be decomposed, or to toxic component, to reduce the toxic side effect of Chinese medicine, is expanded The big application range of Chinese medicine, and microorganism can generate the metabolite of multiplicity, secondary during growth metabolism Metabolite and enzyme, the good drug of these metabolites inherently effect, and the effective component in medicinal material passes through micro- life The transformation of object can form new compound, in short, herb fermenting energy effective component extraction rate improves, curative effect enhancing, can subtract Few certain usage amount.
Comparative example 1:
A kind of preparation method of vegetable protein used for aquiculture, including,
1) rapeseed dregs and/or Cottonseed Meal are pulverized, after ultramicro grinding rapeseed dregs and/or Cottonseed Meal it is whole 60% with The upper partial size with less than 25 μm；
It 2) is 2.5% inoculation yeast bacterium by inoculum concentration, solid state fermentation 2.5d under conditions of fermentation temperature is 27 DEG C.
A kind of feed improving opsariichthys bidens immunity, containing above-mentioned 33 parts of vegetable protein used for aquiculture, include seaweed ketone 2.2 parts of 4 parts of 44 parts of 11 parts of Chinese herbal medicine powder, fish meal, miberal powder, the vitamin of sugar and isomaltoketose.
A kind of preparation method for the feed improving opsariichthys bidens immunity, by vegetable protein used for aquiculture, includes seaweed ketone The Chinese herbal medicine powder of sugar and isomaltoketose, fish meal, miberal powder, vitamin are uniformly mixed, and are granulated to get opsariichthys bidens immunity is improved Feed.
Comparative example 2:
A kind of preparation method of vegetable protein used for aquiculture, including,
1) rapeseed dregs and/or Cottonseed Meal are pulverized, after ultramicro grinding rapeseed dregs and/or Cottonseed Meal it is whole 60% with The upper partial size with less than 25 μm；
2) it is radiated using medium-wave infrared, infrared intensity 5600W/m2, the processing time is 40s；
It 3) is 2.5% inoculation yeast bacterium by inoculum concentration, solid state fermentation 2.5d under conditions of fermentation temperature is 27 DEG C.
A kind of vegetable protein used for aquiculture, the cellulose containing 2-6% in vegetable protein.
A kind of feed improving opsariichthys bidens immunity, contains above-mentioned 33 parts of vegetable protein used for aquiculture, 44 parts of fish meal, mine 4 parts of powder, 2.2 parts of vitamin.
A kind of preparation method for the feed improving opsariichthys bidens immunity, by vegetable protein used for aquiculture, includes seaweed ketone The Chinese herbal medicine powder of sugar and isomaltoketose, fish meal, miberal powder, vitamin are uniformly mixed, and are granulated to get opsariichthys bidens immunity is improved Feed.
Test example 1:
Feeding trial
Breed variety: 1 gram or so of opsariichthys bidens postlarva 3000 is randomly assigned to be five groups: water tank 1, water tank 2,3 and of water tank Water tank 4, each group gross weight is suitable, and every group of net cage always puts jewfish postlarva 500 in a suitable place to breed.Other conditions are essentially identical, and different is Water tank 1 feeds 1 feed of embodiment daily；Water tank 2 feeds 2 feed of embodiment daily；Water tank 3 feeds the feeding of embodiment 3 daily daily Material；Water tank 4 feeds 1 feed of comparative example daily；Water tank 5 feeds 2 feed of comparative example daily；Water tank 6 feeds commercial diet daily.It supports Grow Therapy lasted 1 month, the evaluation result for draining object amount is as shown in table 1, wherein outstanding: compared to net cage 6, the row of every intake It lets out object and is reduced to 1% or more.It is good: to compare net cage 6, the excreta of every intake is reduced to 0.5% or more.It is general: phase Than net cage 6, it was found that excreta reduction effect.Difference: comparing net cage 6, does not find that excreta reduction acts on.Cultivate water turbidity Measurement result is as shown in table 1.
Table 1: the excretion object amount and cultivation water turbidity of opsariichthys bidens cultivation
Classification Excreta reduction effect Turbidity (NTU)
Net cage 1 It is outstanding 1.80
Net cage 2 It is outstanding 1.78
Net cage 3 It is outstanding 1.82
Net cage 4 Difference 2.18
Net cage 5 Generally 2.34
Net cage 6 Difference 5.21
As shown in Table 1, the excreta reduction effect of feed of the embodiment of the present invention is especially significant, this illustrates to adopt in preparation method It takes vegetable protein used for aquiculture made from infra-red radiation to reduce the yield of the excreta of opsariichthys bidens, improves the cleaning of breeding water Degree；Present invention cultivation water turbidity is lower, this, which illustrates to contain in preparation method in feed, takes aquaculture made from infra-red radiation Chinese herbal medicine powder with vegetable protein and comprising trehalulose and isomaltoketose can improve the cleannes of breeding water.
The prior art of routine techniques dawn known to those skilled in the art in above-described embodiment, therefore herein no longer in detail It repeats.
The above embodiments are only used to illustrate the present invention, and not limitation of the present invention, the ordinary skill people of this field Member can also make a variety of changes and modification without departing from the spirit and scope of the present invention.Therefore, all equivalent Technical solution also belong to scope of the invention, scope of patent protection of the invention should be defined by the claims.

Claims (10)

1. a kind of preparation method of vegetable protein used for aquiculture, it is characterised in that: including,
Rapeseed dregs and/or Cottonseed Meal pulverize step；
Infra-red radiation step；And
Solid state fermentation step；
It is described infrared for medium-wave infrared.
2. a kind of preparation method of vegetable protein used for aquiculture according to claim 1, it is characterised in that: the ultra micro Rapeseed dregs and/or Cottonseed Meal are whole after crushing 60% or more has the partial size less than 25 μm.
3. a kind of preparation method of vegetable protein used for aquiculture according to claim 1 or 2, it is characterised in that: described Infrared intensity is 5000-6000W/m2, the processing time is 30-50s.
4. a kind of preparation method of vegetable protein used for aquiculture according to claim 1 or 2, it is characterised in that: described The inoculum concentration of saccharomycete is 2.2-2.7%, and fermentation temperature is 25-30 DEG C, time 2-3d.
5. aquaculture made from a kind of preparation method of vegetable protein used for aquiculture described in claims 1 or 2 or 3 or 4 Use vegetable protein.
6. a kind of vegetable protein used for aquiculture according to claim 5, it is characterised in that: contain in the vegetable protein The cellulose of 2-6%.
7. a kind of feed for improving opsariichthys bidens immunity, it is characterised in that: containing described in claim 5 or 6 used for aquiculture Vegetable protein.
8. a kind of feed for improving opsariichthys bidens immunity according to claim 7, it is characterised in that: the raising opsariichthys bidens The feed of immunity also contains the Chinese herbal medicine powder comprising trehalulose and isomaltoketose, fish meal, miberal powder, microorganism, vitamin.
9. a kind of feed for improving opsariichthys bidens immunity according to claim 8, it is characterised in that: described includes seaweed ketone The Chinese herbal medicine powder of sugar and isomaltoketose is obtained by fermentation process.
10. a kind of preparation method of the feed of raising opsariichthys bidens immunity described in claim 7 or 8 or 9, it is characterised in that: Vegetable protein used for aquiculture, the Chinese herbal medicine powder comprising trehalulose and isomaltoketose, fish meal, miberal powder, vitamin are mixed Uniformly, it is granulated to get the feed of opsariichthys bidens immunity is improved.
CN201811462908.9A 2018-12-03 2018-12-03 A kind of feed and preparation method thereof improving opsariichthys bidens immunity Pending CN109566912A (en)

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CN101558825A (en) * 2009-06-03 2009-10-21 胡国田 Method for producing multi-effect oil-tea-cake meal feed additive
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