CN109566374A - A kind of high yield pea seedlings and its implantation methods - Google Patents
A kind of high yield pea seedlings and its implantation methods Download PDFInfo
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- CN109566374A CN109566374A CN201811608099.8A CN201811608099A CN109566374A CN 109566374 A CN109566374 A CN 109566374A CN 201811608099 A CN201811608099 A CN 201811608099A CN 109566374 A CN109566374 A CN 109566374A
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- Prior art keywords
- pea
- seedlings
- implantation methods
- high yield
- containing nitrogen
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Links
- 238000000034 method Methods 0.000 title claims abstract description 46
- 238000002513 implantation Methods 0.000 title claims abstract description 28
- 240000004713 Pisum sativum Species 0.000 title claims description 135
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 116
- 239000000203 mixture Substances 0.000 claims abstract description 79
- 239000003337 fertilizer Substances 0.000 claims abstract description 58
- 238000009472 formulation Methods 0.000 claims abstract description 58
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 58
- 239000000758 substrate Substances 0.000 claims abstract description 32
- 241000196324 Embryophyta Species 0.000 claims abstract description 8
- 241000219843 Pisum Species 0.000 claims abstract 39
- 235000010582 Pisum sativum Nutrition 0.000 claims description 167
- 230000000844 anti-bacterial effect Effects 0.000 claims description 36
- 239000007788 liquid Substances 0.000 claims description 35
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 27
- 244000045195 Cicer arietinum Species 0.000 claims description 25
- 235000010523 Cicer arietinum Nutrition 0.000 claims description 25
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims description 22
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 21
- 235000013311 vegetables Nutrition 0.000 claims description 20
- DTWJJSICXFENKC-UHFFFAOYSA-N 4-phenyl-1h-benzimidazole Chemical class C=12NC=NC2=CC=CC=1C1=CC=CC=C1 DTWJJSICXFENKC-UHFFFAOYSA-N 0.000 claims description 18
- 229910000323 aluminium silicate Inorganic materials 0.000 claims description 16
- 238000012216 screening Methods 0.000 claims description 16
- DRLFMBDRBRZALE-UHFFFAOYSA-N melatonin Chemical compound COC1=CC=C2NC=C(CCNC(C)=O)C2=C1 DRLFMBDRBRZALE-UHFFFAOYSA-N 0.000 claims description 14
- YJPIGAIKUZMOQA-UHFFFAOYSA-N Melatonin Natural products COC1=CC=C2N(C(C)=O)C=C(CCN)C2=C1 YJPIGAIKUZMOQA-UHFFFAOYSA-N 0.000 claims description 13
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical group [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 claims description 13
- 238000010348 incorporation Methods 0.000 claims description 13
- 241000607479 Yersinia pestis Species 0.000 claims description 12
- 239000007864 aqueous solution Substances 0.000 claims description 12
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 11
- 230000000474 nursing effect Effects 0.000 claims description 11
- 229910000388 diammonium phosphate Inorganic materials 0.000 claims description 10
- 235000019838 diammonium phosphate Nutrition 0.000 claims description 10
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 10
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 10
- 238000002360 preparation method Methods 0.000 claims description 10
- 244000068988 Glycine max Species 0.000 claims description 9
- 235000010469 Glycine max Nutrition 0.000 claims description 9
- 241000238631 Hexapoda Species 0.000 claims description 9
- 229910021536 Zeolite Inorganic materials 0.000 claims description 9
- 235000016709 nutrition Nutrition 0.000 claims description 9
- 230000035764 nutrition Effects 0.000 claims description 9
- 239000007787 solid Substances 0.000 claims description 9
- 239000010457 zeolite Substances 0.000 claims description 9
- 238000001035 drying Methods 0.000 claims description 8
- 229910052901 montmorillonite Inorganic materials 0.000 claims description 7
- 125000003739 carbamimidoyl group Chemical group C(N)(=N)* 0.000 claims description 6
- 150000003672 ureas Chemical class 0.000 claims description 6
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims description 3
- 229910052816 inorganic phosphate Inorganic materials 0.000 claims description 3
- 239000004254 Ammonium phosphate Substances 0.000 claims description 2
- 229910000148 ammonium phosphate Inorganic materials 0.000 claims description 2
- 235000019289 ammonium phosphates Nutrition 0.000 claims description 2
- GUJOJGAPFQRJSV-UHFFFAOYSA-N dialuminum;dioxosilane;oxygen(2-);hydrate Chemical group O.[O-2].[O-2].[O-2].[Al+3].[Al+3].O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O GUJOJGAPFQRJSV-UHFFFAOYSA-N 0.000 claims description 2
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims description 2
- KQPBSBAEBKRAAU-UHFFFAOYSA-N hypochlorous acid;sodium Chemical compound [Na].ClO KQPBSBAEBKRAAU-UHFFFAOYSA-N 0.000 claims 1
- 239000000463 material Substances 0.000 claims 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 abstract description 7
- 229910002651 NO3 Inorganic materials 0.000 abstract description 3
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 abstract description 3
- 230000000050 nutritive effect Effects 0.000 abstract description 3
- 230000001954 sterilising effect Effects 0.000 abstract description 2
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 2
- 239000002699 waste material Substances 0.000 abstract 1
- 238000005286 illumination Methods 0.000 description 14
- 238000002156 mixing Methods 0.000 description 10
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 10
- 239000005708 Sodium hypochlorite Substances 0.000 description 9
- 235000005072 Vigna sesquipedalis Nutrition 0.000 description 8
- 244000090207 Vigna sesquipedalis Species 0.000 description 8
- 239000012153 distilled water Substances 0.000 description 8
- 235000021278 navy bean Nutrition 0.000 description 8
- FFQCWZOGSVKCDN-UHFFFAOYSA-N 2-methyl-4-phenyl-1h-benzimidazole Chemical compound C1=CC=C2NC(C)=NC2=C1C1=CC=CC=C1 FFQCWZOGSVKCDN-UHFFFAOYSA-N 0.000 description 7
- 238000010025 steaming Methods 0.000 description 7
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 206010028980 Neoplasm Diseases 0.000 description 3
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 3
- 201000011510 cancer Diseases 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 206010007247 Carbuncle Diseases 0.000 description 2
- 235000016816 Pisum sativum subsp sativum Nutrition 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000034303 cell budding Effects 0.000 description 2
- 208000001848 dysentery Diseases 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 210000002429 large intestine Anatomy 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 244000003416 Asparagus officinalis Species 0.000 description 1
- 235000005340 Asparagus officinalis Nutrition 0.000 description 1
- 201000009030 Carcinoma Diseases 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 229930191978 Gibberellin Natural products 0.000 description 1
- 208000031361 Hiccup Diseases 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000001142 anti-diarrhea Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 235000005489 dwarf bean Nutrition 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 1
- 239000003448 gibberellin Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 231100000003 human carcinogen Toxicity 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 235000021332 kidney beans Nutrition 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- XKLJHFLUAHKGGU-UHFFFAOYSA-N nitrous amide Chemical compound ON=N XKLJHFLUAHKGGU-UHFFFAOYSA-N 0.000 description 1
- GQPLMRYTRLFLPF-UHFFFAOYSA-N nitrous oxide Inorganic materials [O-][N+]#N GQPLMRYTRLFLPF-UHFFFAOYSA-N 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 230000008855 peristalsis Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 201000004647 tinea pedis Diseases 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 230000008673 vomiting Effects 0.000 description 1
- 210000004885 white matter Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/13—Zeolites
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/30—Growth substrates; Culture media; Apparatus or methods therefor based on or containing synthetic organic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/90—Mixtures of one or more fertilisers with additives not having a specially fertilising activity for affecting the nitrification of ammonium compounds or urea in the soil
Abstract
The present invention provides a kind of high yield pea seedlings and its implantation methods, belong to field of plant cultivation.The present invention passes through sieve kind；Sterilization；Miscegenation；Vernalization；The methods of culture greatly improves pea seedlings yield in the case where guaranteeing that its nutritive value does not reduce.Invention also improves culture substrate, so that it is released formulation fertilizer containing nitrogen while adsorbing the waste nitrate of pea seedlings discharge, can must continually be grown for it.The seven batches of bud ratios of pea seedlings for having used plantation of the invention to come out reach as high as 77.5%, and total phenol content reaches as high as 846.2mg/g.
Description
Technical field
The invention belongs to field of vegetable, especially a kind of high yield pea seedlings and its implantation methods.
Background technique
Pea seedlings: one kind of vegetables.The young stem and leaf of pea and one kind of seedling class vegetables, be otherwise known as " pea point ",
" asparagus ", " tendrils seedling ".It is using the young young stem and leaf of vegetable pea, tender tip as a kind of edible vegetables with green leaves.
Pea seedlings is full of nutrition, containing there are many amino acid needed by human.Its taste faint scent, matter are tender, lubricious agreeable to the taste, color,
Perfume, taste are all good.Nutritive value height and green non-pollution, and faint scent cunning of tasting is tender, delicious flavour uniqueness.For propagandizing hotly, doing
Soup, Shabu pot all be can yet be regarded as the superior vegetables on dining table, by the favor of the majority of consumers.
Enzyme rich in nitrosamine in vitamin C and energy decomposer in the tender leaf of pea pods and bean seedlings, can decompose nitrous
Amine has the function of anti-cancer and cancer-preventing.Pea and general vegetables are different, and contained only wooden fork acid, gibberellin and plant coagulate element etc.
Substance has antibacterial anti-inflammatory, enhances the function of metabolism.Contain dietary fiber more abundant in garden pea and bean seedlings,
Constipation can be prevented, there is gut purge effect.
1. enhancing body's immunity: being rich in the various nutriments of needed by human body in pea, especially contain high-quality egg
The disease resistance and rehabilitation ability of body can be improved in white matter.
2. cancer preventing and treating: being rich in carrotene in pea, the synthesis of human carcinogen's substance can be prevented after edible, to reduce
The formation of cancer cell reduces the disease incidence of human carcinoma.
3. tonneau large intestine: being rich in crude fibre in pea, intestinal peristalsis can be promoted, keep stool smooth, play cleaning large intestine
Effect.Dietary function pea is sweet in flavor, mild-natured, returns spleen, stomach meridian；With gas in benefit, antidiarrheal dysentery, adjust that battalion defends, diuresis, disappear carbuncle
It swells, solve the effect of mammary calculus is malicious；It cures mainly tinea pedis, carbuncle swells, alactation, taste discomfort, hiccup vomiting, trusted subordinate's distending pain, thirsty let out dysentery
Etc. illnesss.
In the prior art, pea seedlings planting technology is broadly divided into two major classes, and one is soil cultivations, and one is plant without soil
Training.But soil cultivation is easy to bring pest and disease damage, soilless cultivation can only generally utilize one batch, not exceed three batches at most.
Chinese patent if application publication number is 103907517 A of CN discloses a kind of very traditional pea seedlings cultivation
Training technology, although which avoids pest and disease damage, but still inevitable stubble number utilizes few problem.
Summary of the invention
For deficiency present in existing pea seedlings planting technology, the present invention is sterilized and is adopted by miscegenation vernalization, bactericidal liquid
With the method for novel culture medium matter, solves pea seedlings subalimentation, few vulnerable to pest and disease damage and regeneration stubble number for a long time
Problem.
Specifically, the present invention is achieved through the following technical solutions:
A kind of high yield pea seedlings implantation methods, which is characterized in that steps are as follows:
(1) sieve kind；
(2) it sterilizes, obtains sterile pea；
(3) miscegenation: Papillionoideae vegetable seeds in addition to pea is mixed to sterile pea, in every 100 sterile peas
Papillionoideae vegetable seeds of incorporation 5-10 in addition to pea, obtains seed mixture；
The Papillionoideae vegetable seeds in addition to pea is killed before incorporation with method identical with sterile pea is obtained
Bacterium；
(4) vernalization: the N- acetyl-5-methoxytryptamine aqueous solution of 10-30 μm of ol/L of configuration is added seed mixture, owns
Seed is spread out, non-overlapping, and after sprouting 2-10 days, the Papillionoideae plant plumule in addition to removing pea obtains pea young shoot；
(5) it cultivates: pea young shoot is transferred in the seedlings nursing plate containing culture substrate, cultivate 5-12 days to get nutrition pea
Bean seedlings.
Preferably, steps are as follows for a kind of high yield pea seedlings implantation methods:
(1) sieve kind: screening grain focuses on 0.12-0.20g, and free from insect pests, nothing break residual, nothing and go rotten, without lopsided pea；
(2) it sterilizes, pea is immersed in bactericidal liquid and is sterilized 24-36 hours, be washed with distilled water once, obtain sterile pea
Beans；
The bactericidal liquid dosage is 5-10 times of pea quality；
(3) miscegenation: Papillionoideae vegetable seeds in addition to pea is mixed to sterile pea, in every 100 sterile peas
Papillionoideae vegetable seeds of incorporation 5-10 in addition to pea, obtains seed mixture；
The Papillionoideae vegetable seeds in addition to pea is killed before incorporation with method identical with sterile pea is obtained
Bacterium；
(4) vernalization: the N- acetyl-5-methoxytryptamine aqueous solution of 10-30 μm of ol/L of configuration is added seed mixture, owns
Seed is spread out, non-overlapping, at 20-25 DEG C, relative humidity 75%-85%, after sprouting 2-10 days under conditions of being protected from light, except removal
Papillionoideae plant plumule outside pea, obtains pea young shoot；
(5) it cultivates: pea young shoot is transferred in the seedlings nursing plate containing culture substrate, cultivate 5-12 days to get nutrition pea
Bean seedlings.
Preferably, the Papillionoideae vegetable seeds in addition to pea is any in Kidney bean, chick-pea and soybean
It is a kind of.
Preferably, it includes alumino-silicate and formulation fertilizer containing nitrogen that the culture substrate, which prepares raw material,；
The alumino-silicate is the mixture of montmorillonite or zeolite or both arbitrary proportion；
The formulation fertilizer containing nitrogen is amidino groups urea derivative.
Preferably, the preparation method of the culture substrate is by formulation fertilizer containing nitrogen, accounts for the inorganic of formulation fertilizer containing nitrogen quality 20-50%
Phosphate and the magnesium sulfate for accounting for formulation fertilizer containing nitrogen quality 20-50% are added in the water of 1-1.2 times of formulation fertilizer containing nitrogen quality, at 50-60 DEG C
Alumino-silicate is added in lower heating 1-2 hours, and additional amount is the 70-90% of formulation fertilizer containing nitrogen quality, and it is small that 2-3 is heated at 60-80 DEG C
When, filter while hot, take solid, at 150-180 DEG C dry 0.5-2 hours to get.
Preferably, the structural formula of the amidino groups urea derivative is as follows:
Preferably, the inorganic phosphate is ammonium phosphate or diammonium hydrogen phosphate.
Preferably, the bactericidal liquid is by sodium hypochlorite: 4- phenylbenzimidazol derivative: water is (0.05- in mass ratio
0.3): (0.05-0.4): 100 mix.
Preferably, the structural formula of the 4- phenylbenzimidazol derivative are as follows:
Wherein R=methyl, hydroxyl or sulfonic group.
When R=hydroxyl, concrete structure formula are as follows:
The nutrition pea seedlings that the implantation methods of any one of the above high yield pea seedlings obtain.
The invention has the beneficial effects that: it is urged using miscegenation advantage and using N- acetyl-5-methoxytryptamine aqueous solution
Bud can promote the nutritive value of pea seedlings；A kind of novel culture substrate is developed, which is adsorbed with dicyandiamidines and spreads out
Biology, amidino groups urea derivative has sterilization and provides the double action of nutrition to pea seedlings, due to pea seedlings meeting during cultivation
Nitrate is slowly released, the alumino-silicate that nitrate can be cultured in matrix slowly adsorbs and slowly displaces absorption originally
Amidino groups urea derivative, to reach slow release effect, so that a batch number can be gathered in by promoting pea seedlings.The culture substrate can also recycle again
It utilizes, formulation fertilizer containing nitrogen is adsorbed again, thus save the cost.
Specific embodiment
The present invention is further elaborated below with reference to specific embodiment, following embodiments are only used to explain
The present invention is not intended to limit the present invention.
Specifically, the instrument or compound source that are related in following embodiments or No. CAS it is as follows:
Pea, Latin name Pisum sativum Linn, kind is green pea.
Ca-montmorillonite is purchased from Lingshou County megalith mineral products processing factory, 50 mesh.
Zeolite is purchased from Henan Zi Jing environment-friendly materials Co., Ltd, 50 mesh.
Fluorescent lamp, power 18W, luminous flux 1800lm.
Sodium hypochlorite, No. CAS: 7681-52-9.
2- methyl 4-phenyl benzimidazole, No. CAS: 110179-50-5.
2- sulfonic group -4- phenylbenzimidazol, No. CAS: 1027179-57-2.
Soybean, Latin name Glycine max (Linn.) Merr., kind is soybean.
Kidney bean, Latin name Phaseolus vulgaris, kind are white flower frame beans.
Chick-pea, Latin name Cicer arietinum, kind are Wushi chick-pea.
N- acetyl-5-methoxytryptamine, No. CAS: 8041-44-9.
Diammonium hydrogen phosphate, No. CAS: 7783-28-0.
Magnesium sulfate, No. CAS: 7487-88-9.
2- methyl 4-phenyl benzimidazole, No. CAS: 110179-50-5.
2- sulfonic group -4- phenylbenzimidazol, No. CAS: 1027179-57-2.
Embodiment 1
A kind of high yield pea seedlings implantation methods, plantation step are as follows:
(1) sieve kind: screening grain focuses on 0.12-0.20g, and free from insect pests, nothing break residual, nothing and go rotten, without lopsided pea, average grain
Weight 0.15g；
(2) it sterilizes, the obtained pea of screening is immersed in bactericidal liquid and is sterilized 24 hours, with the steaming with the quality such as bactericidal liquid
Distilled water washed once, and obtain sterile pea；
The bactericidal liquid dosage is 5 times of pea quality；
The bactericidal liquid is by sodium hypochlorite: 4- phenylbenzimidazol derivative: water is 0.1:0.2:100 mixing in mass ratio
It forms；
The 4- phenylbenzimidazol derivative is 2- methyl 4-phenyl benzimidazole；
(3) miscegenation: soybean is mixed to sterile pea, 7 soybean is mixed in every 100 sterile peas, obtains seed mixture；
The soybean is sterilized before incorporation with method identical with sterile pea is obtained；
(4) vernalization: the N- acetyl-5-methoxytryptamine aqueous solution of 15 μm of ol/L of configuration, addition seed mixture, all kinds
Son is spread out, non-overlapping, and at 20 DEG C, relative humidity 80% after sprouting 7 days under conditions of being protected from light, removes soybean young shoot, obtains pea
Young shoot；
(5) cultivate: pea young shoot is transferred in the seedlings nursing plate containing culture substrate, culture substrate with a thickness of 2cm, adopt
With common fluorescent lamp illumination, daily continuous illumination 12 hours cultivates 7 days in the environment of 25 DEG C, relative humidity 60% to get battalion
Support pea seedlings；
The preparation method of the culture substrate is by the diammonium hydrogen phosphate and 25% of formulation fertilizer containing nitrogen, 30% formulation fertilizer containing nitrogen quality
The magnesium sulfate of formulation fertilizer containing nitrogen is added in the water of 1.1 times of formulation fertilizer containing nitrogen quality, heats 1 hour at 60 DEG C, is addedZeolite adds
Enter 80% that amount is formulation fertilizer containing nitrogen quality, heated at 70 DEG C 2 hours, cross 300 meshes while hot, takes solid, drying 1 at 170 DEG C
Hour to get；
The formulation fertilizer containing nitrogen is
Embodiment 2
A kind of high yield pea seedlings implantation methods, plantation step are as follows:
(1) sieve kind: screening grain focuses on 0.12-0.20g, and free from insect pests, nothing break residual, nothing and go rotten, without lopsided pea, average grain
Weight 0.15g；
(2) it sterilizes, the obtained pea of screening is immersed in bactericidal liquid and is sterilized 24 hours, with the steaming with the quality such as bactericidal liquid
Distilled water washed once, and obtain sterile pea；
The bactericidal liquid dosage is 5 times of pea quality；
The bactericidal liquid is by sodium hypochlorite: 4- phenylbenzimidazol derivative: water is 0.1:0.2:100 mixing in mass ratio
It forms；
The 4- phenylbenzimidazol derivative is 2- methyl 4-phenyl benzimidazole；
(3) miscegenation: Kidney bean is mixed to sterile pea, 7 Kidney beans is mixed in every 100 sterile peas, obtains seed mixture；
The Kidney bean is sterilized before incorporation with method identical with sterile pea is obtained；
(4) vernalization: the N- acetyl-5-methoxytryptamine aqueous solution of 15 μm of ol/L of configuration, addition seed mixture, all kinds
Son is spread out, non-overlapping, and at 20 DEG C, relative humidity 80% after sprouting 7 days under conditions of being protected from light, removes Kidney bean young shoot, obtains pea
Young shoot；
(5) cultivate: pea young shoot is transferred in the seedlings nursing plate containing culture substrate, culture substrate with a thickness of 2cm, adopt
With common fluorescent lamp illumination, daily continuous illumination 12 hours cultivates 7 days in the environment of 25 DEG C, relative humidity 60% to get battalion
Support pea seedlings；
The preparation method of the culture substrate is by the diammonium hydrogen phosphate and 25% of formulation fertilizer containing nitrogen, 30% formulation fertilizer containing nitrogen quality
The magnesium sulfate of formulation fertilizer containing nitrogen is added in the water of 1.1 times of formulation fertilizer containing nitrogen quality, heats 1 hour at 60 DEG C, is addedZeolite adds
Enter 80% that amount is formulation fertilizer containing nitrogen quality, heated at 70 DEG C 2 hours, cross 300 meshes while hot, takes solid, drying 1 at 170 DEG C
Hour to get；
The formulation fertilizer containing nitrogen is
Embodiment 3
A kind of high yield pea seedlings implantation methods, plantation step are as follows:
(1) sieve kind: screening grain focuses on 0.12-0.20g, and free from insect pests, nothing break residual, nothing and go rotten, without lopsided pea, average grain
Weight 0.15g；
(2) it sterilizes, the obtained pea of screening is immersed in bactericidal liquid and is sterilized 24 hours, with the steaming with the quality such as bactericidal liquid
Distilled water washed once, and obtain sterile pea；
The bactericidal liquid dosage is 5 times of pea quality；
The bactericidal liquid is by sodium hypochlorite: 4- phenylbenzimidazol derivative: water is 0.1:0.2:100 mixing in mass ratio
It forms；
The 4- phenylbenzimidazol derivative is 2- methyl 4-phenyl benzimidazole；
(3) miscegenation: chick-pea is mixed to sterile pea, 7 chick-pea is mixed in every 100 sterile peas, obtains hybrid
Son；
The chick-pea is sterilized before incorporation with method identical with sterile pea is obtained；
(4) vernalization: the N- acetyl-5-methoxytryptamine aqueous solution of 15 μm of ol/L of configuration, addition seed mixture, all kinds
Son is spread out, non-overlapping, and at 20 DEG C, relative humidity 80% after sprouting 7 days under conditions of being protected from light, removes chick-pea young shoot, obtains pea
Beans young shoot；
(5) cultivate: pea young shoot is transferred in the seedlings nursing plate containing culture substrate, culture substrate with a thickness of 2cm, adopt
With common fluorescent lamp illumination, daily continuous illumination 12 hours cultivates 7 days in the environment of 25 DEG C, relative humidity 60% to get battalion
Support pea seedlings；
The preparation method of the culture substrate is by the diammonium hydrogen phosphate and 25% of formulation fertilizer containing nitrogen, 30% formulation fertilizer containing nitrogen quality
The magnesium sulfate of formulation fertilizer containing nitrogen is added in the water of 1.1 times of formulation fertilizer containing nitrogen quality, heats 1 hour at 60 DEG C, is addedZeolite adds
Enter 80% that amount is formulation fertilizer containing nitrogen quality, heated at 70 DEG C 2 hours, cross 300 meshes while hot, takes solid, drying 1 at 170 DEG C
Hour to get；
The formulation fertilizer containing nitrogen is
Embodiment 4
A kind of high yield pea seedlings implantation methods, plantation step are as follows:
(1) sieve kind: screening grain focuses on 0.12-0.20g, and free from insect pests, nothing break residual, nothing and go rotten, without lopsided pea, average grain
Weight 0.15g；
(2) it sterilizes, the obtained pea of screening is immersed in bactericidal liquid and is sterilized 24 hours, with the steaming with the quality such as bactericidal liquid
Distilled water washed once, and obtain sterile pea；
The bactericidal liquid dosage is 5 times of pea quality；
The bactericidal liquid is by sodium hypochlorite: 4- phenylbenzimidazol derivative: water is 0.1:0.2:100 mixing in mass ratio
It forms；
The 4- phenylbenzimidazol derivative is 2- methyl 4-phenyl benzimidazole；
(3) miscegenation: chick-pea is mixed to sterile pea, 7 chick-pea is mixed in every 100 sterile peas, obtains hybrid
Son；
The chick-pea is sterilized before incorporation with method identical with sterile pea is obtained；
(4) vernalization: the N- acetyl-5-methoxytryptamine aqueous solution of 15 μm of ol/L of configuration, addition seed mixture, all kinds
Son is spread out, non-overlapping, and at 20 DEG C, relative humidity 80% after sprouting 7 days under conditions of being protected from light, removes chick-pea young shoot, obtains pea
Beans young shoot；
(5) cultivate: pea young shoot is transferred in the seedlings nursing plate containing culture substrate, culture substrate with a thickness of 2cm, adopt
With common fluorescent lamp illumination, daily continuous illumination 12 hours cultivates 7 days in the environment of 25 DEG C, relative humidity 60% to get battalion
Support pea seedlings；
The preparation method of the culture substrate is by the diammonium hydrogen phosphate and 25% of formulation fertilizer containing nitrogen, 30% formulation fertilizer containing nitrogen quality
The magnesium sulfate of formulation fertilizer containing nitrogen is added in the water of 1.1 times of formulation fertilizer containing nitrogen quality, heats 1 hour at 60 DEG C, and ca-montmorillonite is added,
Additional amount is the 80% of formulation fertilizer containing nitrogen quality, heats 2 hours at 70 DEG C, crosses 300 meshes while hot, takes solid, drying at 170 DEG C
1 hour to get；
The formulation fertilizer containing nitrogen is
Embodiment 5
A kind of high yield pea seedlings implantation methods, plantation step are as follows:
(1) sieve kind: screening grain focuses on 0.12-0.20g, and free from insect pests, nothing break residual, nothing and go rotten, without lopsided pea, average grain
Weight 0.15g；
(2) it sterilizes, the obtained pea of screening is immersed in bactericidal liquid and is sterilized 24 hours, with the steaming with the quality such as bactericidal liquid
Distilled water washed once, and obtain sterile pea；
The bactericidal liquid dosage is 5 times of pea quality；
The bactericidal liquid is by sodium hypochlorite: 4- phenylbenzimidazol derivative: water is 0.1:0.2:100 mixing in mass ratio
It forms；
The 4- phenylbenzimidazol derivative is 2- methyl 4-phenyl benzimidazole；
(3) miscegenation: chick-pea is mixed to sterile pea, 7 chick-pea is mixed in every 100 sterile peas, obtains hybrid
Son；
The chick-pea is sterilized before incorporation with method identical with sterile pea is obtained；
(4) vernalization: the N- acetyl-5-methoxytryptamine aqueous solution of 15 μm of ol/L of configuration, addition seed mixture, all kinds
Son is spread out, non-overlapping, and at 20 DEG C, relative humidity 80% after sprouting 7 days under conditions of being protected from light, removes chick-pea young shoot, obtains pea
Beans young shoot；
(5) cultivate: pea young shoot is transferred in the seedlings nursing plate containing culture substrate, culture substrate with a thickness of 2cm, adopt
With common fluorescent lamp illumination, daily continuous illumination 12 hours cultivates 7 days in the environment of 25 DEG C, relative humidity 60% to get battalion
Support pea seedlings；
The preparation method of the culture substrate is by the diammonium hydrogen phosphate and 25% of formulation fertilizer containing nitrogen, 30% formulation fertilizer containing nitrogen quality
The magnesium sulfate of formulation fertilizer containing nitrogen is added in the water of 1.1 times of formulation fertilizer containing nitrogen quality, heats 1 hour at 60 DEG C, and alumino-silicate is added, adds
Enter 80% that amount is formulation fertilizer containing nitrogen quality, heated at 70 DEG C 2 hours, cross 300 meshes while hot, takes solid, drying 1 at 170 DEG C
Hour to get；
The formulation fertilizer containing nitrogen is
The alumino-silicate isThe mixing alumino-silicate that zeolite and ca-montmorillonite mass ratio are 2.5:1.
Embodiment 6
A kind of high yield pea seedlings implantation methods, plantation step are as follows:
(1) sieve kind: screening grain focuses on 0.12-0.20g, and free from insect pests, nothing break residual, nothing and go rotten, without lopsided pea, average grain
Weight 0.15g；
(2) it sterilizes, the obtained pea of screening is immersed in bactericidal liquid and is sterilized 24 hours, with the steaming with the quality such as bactericidal liquid
Distilled water washed once, and obtain sterile pea；
The bactericidal liquid dosage is 5 times of pea quality；
The bactericidal liquid is by sodium hypochlorite: 4- phenylbenzimidazol derivative: water is 0.1:0.2:100 mixing in mass ratio
It forms；
The 4- phenylbenzimidazol derivative structural formula are as follows:
(3) miscegenation: chick-pea is mixed to sterile pea, 7 chick-pea is mixed in every 100 sterile peas, obtains hybrid
Son；
The chick-pea is sterilized before incorporation with method identical with sterile pea is obtained；
(4) vernalization: the N- acetyl-5-methoxytryptamine aqueous solution of 15 μm of ol/L of configuration, addition seed mixture, all kinds
Son is spread out, non-overlapping, and at 20 DEG C, relative humidity 80% after sprouting 7 days under conditions of being protected from light, removes chick-pea young shoot, obtains pea
Beans young shoot；
(5) cultivate: pea young shoot is transferred in the seedlings nursing plate containing culture substrate, culture substrate with a thickness of 2cm, adopt
With common fluorescent lamp illumination, daily continuous illumination 12 hours cultivates 7 days in the environment of 25 DEG C, relative humidity 60% to get battalion
Support pea seedlings；
The preparation method of the culture substrate is by the diammonium hydrogen phosphate and 25% of formulation fertilizer containing nitrogen, 30% formulation fertilizer containing nitrogen quality
The magnesium sulfate of formulation fertilizer containing nitrogen is added in the water of 1.1 times of formulation fertilizer containing nitrogen quality, heats 1 hour at 60 DEG C, and alumino-silicate is added, adds
Enter 80% that amount is formulation fertilizer containing nitrogen quality, heated at 70 DEG C 2 hours, cross 300 meshes while hot, takes solid, drying 1 at 170 DEG C
Hour to get；
The formulation fertilizer containing nitrogen is
The alumino-silicate isThe mixing alumino-silicate that zeolite and ca-montmorillonite mass ratio are 2.5:1.
Embodiment 7
A kind of high yield pea seedlings implantation methods, plantation step are as follows:
(1) sieve kind: screening grain focuses on 0.12-0.20g, and free from insect pests, nothing break residual, nothing and go rotten, without lopsided pea, average grain
Weight 0.15g；
(2) it sterilizes, the obtained pea of screening is immersed in bactericidal liquid and is sterilized 24 hours, with the steaming with the quality such as bactericidal liquid
Distilled water washed once, and obtain sterile pea；
The bactericidal liquid dosage is 5 times of pea quality；
The bactericidal liquid is by sodium hypochlorite: 4- phenylbenzimidazol derivative: water is 0.1:0.2:100 mixing in mass ratio
It forms；
The 4- phenylbenzimidazol derivative are as follows: 2- sulfonic group -4- phenylbenzimidazol；
(3) miscegenation: chick-pea is mixed to sterile pea, 7 chick-pea is mixed in every 100 sterile peas, obtains hybrid
Son；
The chick-pea is sterilized before incorporation with method identical with sterile pea is obtained；
(4) vernalization: the N- acetyl-5-methoxytryptamine aqueous solution of 15 μm of ol/L of configuration, addition seed mixture, all kinds
Son is spread out, non-overlapping, and at 20 DEG C, relative humidity 80% after sprouting 7 days under conditions of being protected from light, removes chick-pea young shoot, obtains pea
Beans young shoot；
(5) cultivate: pea young shoot is transferred in the seedlings nursing plate containing culture substrate, culture substrate with a thickness of 2cm, adopt
With common fluorescent lamp illumination, daily continuous illumination 12 hours cultivates 7 days in the environment of 25 DEG C, relative humidity 60% to get battalion
Support pea seedlings；
The preparation method of the culture substrate is by the diammonium hydrogen phosphate and 25% of formulation fertilizer containing nitrogen, 30% formulation fertilizer containing nitrogen quality
The magnesium sulfate of formulation fertilizer containing nitrogen is added in the water of 1.1 times of formulation fertilizer containing nitrogen quality, heats 1 hour at 60 DEG C, and alumino-silicate is added, adds
Enter 80% that amount is formulation fertilizer containing nitrogen quality, heated at 70 DEG C 2 hours, cross 300 meshes while hot, takes solid, drying 1 at 170 DEG C
Hour to get；
The formulation fertilizer containing nitrogen is
The alumino-silicate isThe mixing alumino-silicate that zeolite and ca-montmorillonite mass ratio are 2.5:1.
Test case 1
Bud ratio statistics
1000 pea seedlings of each plantation, the budding of three batches, five batches and seven batches of statistics are planted to according to embodiment 1-7 method
Rate.It is still pressed old terms culture 5 days after pea seedlings harvesting, 5 days endogenous budding height, which do not sprout to be considered as less than 5cm or, does not sprout, and
It removes.Statistics radix is 1000 when calculating bud ratio.
Statistical result is as shown in table 1.
1 bud ratio of table
| Three batches of bud ratios (%) | Five batches of bud ratios (%) | Seven batches of bud ratios (%) | |
| Embodiment 1 | 95.4 | 65.4 | 42.6 |
| Embodiment 2 | 96.3 | 66.2 | 45.9 |
| Embodiment 3 | 97.0 | 73.9 | 55.0 |
| Embodiment 4 | 97.7 | 73.5 | 54.4 |
| Embodiment 5 | 98.8 | 80.7 | 68.4 |
| Embodiment 6 | 99.3 | 82.5 | 71.5 |
| Embodiment 7 | 99.4 | 84.3 | 79.5 |
As seen from the above table, pea seedlings implantation methods provided by the invention have very high bud ratio, traditional implantation methods one
It can only go out as soon as batch, relatively advanced method generally also goes out three batches in the prior art, but method provided by the invention is universal
It can go out to five batches, most 77.5% can go out to seven batches, far beyond the prior art.
Test case 2
The measurement of pea seedlings total phenol content
The pea seedlings first planting total phenol content of embodiment 1-7 cultivation is measured, measuring method is Folin-Ciocalteu colorimetric
Method.
Test result is as shown in table 2.
2 total phenol content of table
| Total phenol content (mg/g) | |
| Embodiment 1 | 686.4 |
| Embodiment 2 | 692.3 |
| Embodiment 3 | 725.3 |
| Embodiment 4 | 739.0 |
| Embodiment 5 | 794.8 |
| Embodiment 6 | 812.9 |
| Embodiment 7 | 846.2 |
As seen from the above table, the pea seedlings that the pea seedlings implantation methods provided through the invention are planted has good nutrition
Value, total phenol content is more than 650mg/g.The implantation methods wherein especially provided with embodiment 7 are best, obtained first planting pea
Seedling total phenol content can be up to 846.2mg/g.
Claims (10)
1. a kind of high yield pea seedlings implantation methods, which is characterized in that steps are as follows:
(1) sieve kind；
(2) it sterilizes, obtains sterile pea；
(3) miscegenation: the Papillionoideae vegetable seeds in addition to pea is mixed to sterile pea, is mixed in every 100 sterile peas
The 5-10 Papillionoideae vegetable seeds in addition to pea obtain seed mixture；
The Papillionoideae vegetable seeds in addition to pea is sterilized before incorporation with method identical with sterile pea is obtained；
(4) vernalization: seed mixture, all seeds are added in the N- acetyl-5-methoxytryptamine aqueous solution of 10-30 μm of ol/L of configuration
It spreads out, non-overlapping, after sprouting 2-10 days, the Papillionoideae plant plumule in addition to removing pea obtains pea young shoot；
(5) it cultivates: pea young shoot is transferred in the seedlings nursing plate containing culture substrate, cultivate 5-12 days to get nutrition pea seedlings.
2. a kind of high yield pea seedlings implantation methods, which is characterized in that steps are as follows:
(1) sieve kind: screening grain focuses on 0.12-0.20g, and free from insect pests, nothing break residual, nothing and go rotten, without lopsided pea；
(2) it sterilizes, pea is immersed in bactericidal liquid and is sterilized 24-36 hours, be washed with water once, obtain sterile pea；
The bactericidal liquid dosage is 5-10 times of pea quality；
(3) miscegenation: the Papillionoideae vegetable seeds in addition to pea is mixed to sterile pea, is mixed in every 100 sterile peas
The 5-10 Papillionoideae vegetable seeds in addition to pea obtain seed mixture；
The Papillionoideae vegetable seeds in addition to pea is sterilized before incorporation with method identical with sterile pea is obtained；
(4) vernalization: seed mixture, all seeds are added in the N- acetyl-5-methoxytryptamine aqueous solution of 10-30 μm of ol/L of configuration
Spread out, it is non-overlapping, at 20-25 DEG C, relative humidity 75%-85%, after being sprouted 2-10 days under conditions of being protected from light, except removal pea
Outer Papillionoideae plant plumule, obtains pea young shoot；
(5) it cultivates: pea young shoot is transferred in the seedlings nursing plate containing culture substrate, cultivate 5-12 days to get nutrition pea seedlings.
3. high yield pea seedlings implantation methods according to claim 1 or 2, which is characterized in that the butterfly in addition to pea
Shape flower subfamily plant seed is any one in Kidney bean, chick-pea and soybean.
4. high yield pea seedlings implantation methods according to claim 1 or 2, which is characterized in that the culture substrate preparation is former
Material includes alumino-silicate and formulation fertilizer containing nitrogen；
The alumino-silicate is montmorillonite or zeolite or both with the mixture of arbitrary proportion；
The formulation fertilizer containing nitrogen is amidino groups urea derivative.
5. high yield pea seedlings implantation methods according to claim 4, which is characterized in that the preparation method of the culture substrate
For by formulation fertilizer containing nitrogen, account for the inorganic phosphate of formulation fertilizer containing nitrogen quality 20-50% and account for the magnesium sulfate of formulation fertilizer containing nitrogen quality 20-50%
It is added in the water of 1-1.2 times of formulation fertilizer containing nitrogen quality, is heated 1-2 hours at 50-60 DEG C, alumino-silicate is added, additional amount is
The 70-90% of formulation fertilizer containing nitrogen quality is heated 2-3 hours at 60-80 DEG C, is filtered while hot, takes solid, drying at 150-180 DEG C
0.5-2 hours to get.
6. high yield pea seedlings implantation methods according to claim 4, which is characterized in that the structure of the amidino groups urea derivative
Formula is as follows:
7. high yield pea seedlings implantation methods according to claim 5, which is characterized in that the inorganic phosphate is ammonium phosphate
Or diammonium hydrogen phosphate.
8. the implantation methods of high yield pea seedlings according to claim 2, which is characterized in that the bactericidal liquid is by hypochlorous acid
Sodium: 4- phenylbenzimidazol derivative: water is (0.05-0.3) in mass ratio: (0.05-0.4): 100 mix.
9. the implantation methods of high yield pea seedlings according to claim 8, which is characterized in that the 4- phenylbenzimidazol spreads out
The structural formula of biology are as follows:
Wherein R=methyl, hydroxyl or sulfonic group.
10. the nutrition pea seedlings that the implantation methods of high yield pea seedlings described in claim 1-9 any one obtain.
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