CN109566351A - A kind of culture substrate improving bud ratio - Google Patents
A kind of culture substrate improving bud ratio Download PDFInfo
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- CN109566351A CN109566351A CN201811608086.0A CN201811608086A CN109566351A CN 109566351 A CN109566351 A CN 109566351A CN 201811608086 A CN201811608086 A CN 201811608086A CN 109566351 A CN109566351 A CN 109566351A
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- pea
- culture substrate
- containing nitrogen
- fertilizer containing
- bud ratio
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/30—Growth substrates; Culture media; Apparatus or methods therefor based on or containing synthetic organic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/13—Zeolites
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Soil Sciences (AREA)
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Fertilizers (AREA)
Abstract
The present invention provides a kind of culture substrates and preparation method thereof for improving bud ratio.The culture substrate for improving bud ratio the preparation method comprises the following steps: formulation fertilizer containing nitrogen, inorganic phosphate and magnesium sulfate are added to the water, heat 1-2 hours at 50-60 DEG C, addition alumino-silicate, it is heated 2-3 hours at 60-80 DEG C, filter while hot, take solid dry to get.Compared with prior art, culture substrate of the invention greatly improves pea seedlings yield in the case where the nutritive value for ensuring pea seedlings does not reduce.Moreover, culture substrate of the invention releases formulation fertilizer containing nitrogen while adsorbing the waste nitrate of pea seedlings discharge, can must continually grow for it.The seven batches of bud ratios of pea seedlings for having used plantation of the invention to come out reach as high as 77.5%, and total phenol content reaches as high as 846.2mg/g.
Description
Technical field
The invention belongs to field of vegetable, especially a kind of culture substrate for improving bud ratio.
Background technique
Pea seedlings: one kind of vegetables.The young stem and leaf of pea and one kind of seedling class vegetables, be otherwise known as " pea point ",
" asparagus ", " tendrils seedling ".It is using the young young stem and leaf of vegetable pea, tender tip as a kind of edible vegetables with green leaves.
Pea seedlings is full of nutrition, containing there are many amino acid needed by human.Its taste faint scent, matter are tender, lubricious agreeable to the taste, color,
Perfume, taste are all good.Nutritive value height and green non-pollution, and faint scent cunning of tasting is tender, delicious flavour uniqueness.For propagandizing hotly, doing
Soup, Shabu pot all be can yet be regarded as the superior vegetables on dining table, by the favor of the majority of consumers.
Enzyme rich in nitrosamine in vitamin C and energy decomposer in the tender leaf of pea pods and bean seedlings, can decompose nitrous
Amine has the function of anti-cancer and cancer-preventing.Pea and general vegetables are different, and contained only wooden fork acid, gibberellin and plant coagulate element etc.
Substance has antibacterial anti-inflammatory, enhances the function of metabolism.Contain dietary fiber more abundant in garden pea and bean seedlings,
Constipation can be prevented, there is gut purge effect.
1. enhancing body's immunity: being rich in the various nutriments of needed by human body in pea, especially contain high-quality egg
The disease resistance and rehabilitation ability of body can be improved in white matter.
2. cancer preventing and treating: being rich in carrotene in pea, the synthesis of human carcinogen's substance can be prevented after edible, to reduce
The formation of cancer cell reduces the disease incidence of human carcinoma.
3. tonneau large intestine: being rich in crude fibre in pea, intestinal peristalsis can be promoted, keep stool smooth, play cleaning large intestine
Effect.Dietary function pea is sweet in flavor, mild-natured, returns spleen, stomach meridian；With gas in benefit, antidiarrheal dysentery, adjust that battalion defends, diuresis, disappear carbuncle
It swells, solve the effect of mammary calculus is malicious；It cures mainly tinea pedis, carbuncle swells, alactation, taste discomfort, hiccup vomiting, trusted subordinate's distending pain, thirsty let out dysentery
Etc. illnesss.
In the prior art, pea seedlings planting technology is broadly divided into two major classes, and one is soil cultivations, and one is plant without soil
Training.But soil cultivation is easy to bring pest and disease damage, soilless cultivation can only generally utilize one batch, not exceed three batches at most.
Chinese patent if application publication number is 103907517 A of CN discloses a kind of very traditional pea seedlings cultivation
Training technology, although which avoids pest and disease damage, but still inevitable stubble number utilizes few problem.
The present invention provides a kind of culture substrate for improving bud ratio, is adsorbed with amidino groups urea derivative, amidino groups urea derivative tool
There is sterilization and provide the double action of nutrition to pea seedlings, releases nitrate since pea seedlings will be slow during cultivation, nitre
The alumino-silicate that hydrochlorate can be cultured in matrix slowly adsorbs and slowly displaces the amidino groups urea derivative of absorption originally, to reach
At slow release effect, so that a batch number can be gathered in by promoting pea seedlings.The culture substrate can adsorb again containing nitrogenous fertilizers with recycling and reusing
Material, thus save the cost.
Summary of the invention
The purpose of the present invention is to provide a kind of culture substrates and preparation method thereof for improving bud ratio.Solve for a long time with
Come pea seedlings subalimentation, the problem few vulnerable to pest and disease damage and regeneration stubble number.
Specifically, the present invention is achieved through the following technical solutions:
A kind of preparation method for the culture substrate improving bud ratio, includes the following steps: formulation fertilizer containing nitrogen, inorganic phosphate
It is added to the water with magnesium sulfate, is heated 1-2 hours at 50-60 DEG C, alumino-silicate is added, it is small that 2-3 is heated at 60-80 DEG C
When, filter while hot, take solid dry to get.
It is further preferred that a kind of preparation method for the culture substrate for improving bud ratio, includes the following steps: containing nitrogenous fertilizers
Expect, the magnesium sulfate that accounts for the inorganic phosphate of formulation fertilizer containing nitrogen quality 20-50% and account for formulation fertilizer containing nitrogen quality 20-50% is added to 1-
It in the water of 1.2 times of formulation fertilizer containing nitrogen quality, is heated 1-2 hours at 50-60 DEG C, alumino-silicate is added, additional amount is formulation fertilizer containing nitrogen
The 70-90% of quality is heated 2-3 hours at 60-80 DEG C, is filtered while hot, takes solid, dry 0.5-2 is small at 150-180 DEG C
When to get.
The formulation fertilizer containing nitrogen is amidino groups urea derivative.
The structural formula of the amidino groups urea derivative is as follows:
The inorganic phosphate is ammonium phosphate or diammonium hydrogen phosphate.
The alumino-silicate is the mixture of montmorillonite or zeolite or both arbitrary proportion.
The invention also discloses a kind of culture substrates for improving bud ratio, using the culture substrate of above-mentioned raising bud ratio
Preparation method be prepared.
The invention also discloses application of the culture substrate of above-mentioned raising bud ratio in the plantation of high yield pea seedlings.
Concrete application is as follows:
Steps are as follows for a kind of high yield pea seedlings implantation methods:
(1) sieve kind: screening grain focuses on 0.12-0.20g, and free from insect pests, nothing break residual, nothing and go rotten, without lopsided pea；
(2) it sterilizes, pea is immersed in bactericidal liquid and is sterilized 24-36 hours, be washed with distilled water once, obtain sterile pea
Beans；
The bactericidal liquid dosage is 5-10 times of pea quality；
(3) miscegenation: Papillionoideae vegetable seeds in addition to pea is mixed to sterile pea, in every 100 sterile peas
Papillionoideae vegetable seeds of incorporation 5-10 in addition to pea, obtains seed mixture；
The Papillionoideae vegetable seeds in addition to pea is killed before incorporation with method identical with sterile pea is obtained
Bacterium；
(4) vernalization: the N- acetyl-5-methoxytryptamine aqueous solution of 10-30 μm of ol/L of configuration is added seed mixture, owns
Seed is spread out, non-overlapping, at 20-25 DEG C, relative humidity 75%-85%, after sprouting 2-10 days under conditions of being protected from light, except removal
Papillionoideae plant plumule outside pea, obtains pea young shoot；
(5) it cultivates: pea young shoot is transferred in the seedlings nursing plate containing culture substrate, cultivate 5-12 days to get nutrition pea
Bean seedlings.
Preferably, the Papillionoideae vegetable seeds in addition to pea is any in Kidney bean, chick-pea and soybean
It is a kind of.
Preferably, the bactericidal liquid is by sodium hypochlorite: 4- phenylbenzimidazol derivative: water is (0.05- in mass ratio
0.3): (0.05-0.4): 100 mix.
Preferably, the structural formula of the 4- phenylbenzimidazol derivative are as follows:
Wherein R=methyl, hydroxyl or sulfonic group.
When R=hydroxyl, concrete structure formula are as follows:
The invention has the beneficial effects that: a kind of novel culture substrate is developed, which is adsorbed with dicyandiamidines
Derivative, amidino groups urea derivative has sterilization and provides the double action of nutrition to pea seedlings, since pea seedlings is during cultivation
It will be slow and release nitrate, the alumino-silicate that nitrate can be cultured in matrix is slowly adsorbed and slowly displaced and adsorbs originally
Amidino groups urea derivative, to reach slow release effect, so that a batch number can be gathered in by promoting pea seedlings.The culture substrate can also recycle
It recycles, formulation fertilizer containing nitrogen is adsorbed again, thus save the cost.
Specific embodiment
The present invention is further elaborated below with reference to specific embodiment, following embodiments are only used to explain
The present invention is not intended to limit the present invention.
Specifically, the instrument or compound source that are related in following embodiments or No. CAS it is as follows:
Pea, Latin name Pisum sativum Linn, kind is green pea.
Ca-montmorillonite is purchased from Lingshou County megalith mineral products processing factory, 50 mesh.
Zeolite is purchased from Henan Zi Jing environment-friendly materials Co., Ltd, 50 mesh.
Fluorescent lamp, power 18W, luminous flux 1800lm.
Sodium hypochlorite, No. CAS: 7681-52-9.
2- methyl 4-phenyl benzimidazole, No. CAS: 110179-50-5.
2- sulfonic group -4- phenylbenzimidazol, No. CAS: 1027179-57-2.
Soybean, Latin name Glycine max (Linn.) Merr., kind is soybean.
Kidney bean, Latin name Phaseolus vulgaris, kind are white flower frame beans.
Chick-pea, Latin name Cicer arietinum, kind are Wushi chick-pea.
N- acetyl-5-methoxytryptamine, No. CAS: 8041-44-9.
Diammonium hydrogen phosphate, No. CAS: 7783-28-0.
Magnesium sulfate, No. CAS: 7487-88-9.
No. CAS: 570410-72-9.
No. CAS: 1075753-20-6.
2- methyl 4-phenyl benzimidazole, No. CAS: 110179-50-5.
2- sulfonic group -4- phenylbenzimidazol, No. CAS: 1027179-57-2.
Embodiment 1
A kind of high yield pea seedlings implantation methods, plantation step are as follows:
(1) sieve kind: screening grain focuses on 0.12-0.20g, and free from insect pests, nothing break residual, nothing and go rotten, without lopsided pea, average grain
Weight 0.15g；
(2) it sterilizes, the obtained pea of screening is immersed in bactericidal liquid and is sterilized 24 hours, with the steaming with the quality such as bactericidal liquid
Distilled water washed once, and obtain sterile pea；
The bactericidal liquid dosage is 5 times of pea quality；
The bactericidal liquid is by sodium hypochlorite: 4- phenylbenzimidazol derivative: water is 0.1:0.2:100 mixing in mass ratio
It forms；
The 4- phenylbenzimidazol derivative is 2- methyl 4-phenyl benzimidazole；
(3) miscegenation: soybean is mixed to sterile pea, 7 soybean is mixed in every 100 sterile peas, obtains seed mixture；
The soybean is sterilized before incorporation with method identical with sterile pea is obtained；
(4) vernalization: the N- acetyl-5-methoxytryptamine aqueous solution of 15 μm of ol/L of configuration, addition seed mixture, all kinds
Son is spread out, non-overlapping, and at 20 DEG C, relative humidity 80% after sprouting 7 days under conditions of being protected from light, removes soybean young shoot, obtains pea
Young shoot；
(5) it cultivates: pea young shoot is transferred in the seedlings nursing plate containing the culture substrate for being improved bud ratio, it is described to improve out
The culture substrate of bud rate with a thickness of 2cm, using common fluorescent lamp illumination, daily continuous illumination 12 hours is relatively wet at 25 DEG C
7 days are cultivated in the environment of degree 60% to get nutrition pea seedlings；
The preparation method of the culture substrate for improving bud ratio is by the phosphoric acid of formulation fertilizer containing nitrogen, 30% formulation fertilizer containing nitrogen quality
The magnesium sulfate of diammonium hydrogen and 25% formulation fertilizer containing nitrogen is added in the water of 1.1 times of formulation fertilizer containing nitrogen quality, heats 1 hour, adds at 60 DEG C
EnterZeolite, additional amount are the 80% of formulation fertilizer containing nitrogen quality, heat 2 hours at 70 DEG C, cross 300 meshes while hot, take solid,
At 170 DEG C dry 1 hour to get；
The formulation fertilizer containing nitrogen is
Embodiment 2
A kind of high yield pea seedlings implantation methods, plantation step are as follows:
(1) sieve kind: screening grain focuses on 0.12-0.20g, and free from insect pests, nothing break residual, nothing and go rotten, without lopsided pea, average grain
Weight 0.15g；
(2) it sterilizes, the obtained pea of screening is immersed in bactericidal liquid and is sterilized 24 hours, with the steaming with the quality such as bactericidal liquid
Distilled water washed once, and obtain sterile pea；
The bactericidal liquid dosage is 5 times of pea quality；
The bactericidal liquid is by sodium hypochlorite: 4- phenylbenzimidazol derivative: water is 0.1:0.2:100 mixing in mass ratio
It forms；
The 4- phenylbenzimidazol derivative is 2- methyl 4-phenyl benzimidazole；
(3) miscegenation: Kidney bean is mixed to sterile pea, 7 Kidney beans is mixed in every 100 sterile peas, obtains seed mixture；
The Kidney bean is sterilized before incorporation with method identical with sterile pea is obtained；
(4) vernalization: the N- acetyl-5-methoxytryptamine aqueous solution of 15 μm of ol/L of configuration, addition seed mixture, all kinds
Son is spread out, non-overlapping, and at 20 DEG C, relative humidity 80% after sprouting 7 days under conditions of being protected from light, removes Kidney bean young shoot, obtains pea
Young shoot；
(5) it cultivates: pea young shoot is transferred in the seedlings nursing plate containing the culture substrate for being improved bud ratio, it is described to improve out
The culture substrate of bud rate with a thickness of 2cm, using common fluorescent lamp illumination, daily continuous illumination 12 hours is relatively wet at 25 DEG C
7 days are cultivated in the environment of degree 60% to get nutrition pea seedlings；
The preparation method of the culture substrate for improving bud ratio is by the phosphoric acid of formulation fertilizer containing nitrogen, 30% formulation fertilizer containing nitrogen quality
The magnesium sulfate of diammonium hydrogen and 25% formulation fertilizer containing nitrogen is added in the water of 1.1 times of formulation fertilizer containing nitrogen quality, heats 1 hour, adds at 60 DEG C
EnterZeolite, additional amount are the 80% of formulation fertilizer containing nitrogen quality, heat 2 hours at 70 DEG C, cross 300 meshes while hot, take solid,
At 170 DEG C dry 1 hour to get；
The formulation fertilizer containing nitrogen is
Embodiment 3
A kind of high yield pea seedlings implantation methods, plantation step are as follows:
(1) sieve kind: screening grain focuses on 0.12-0.20g, and free from insect pests, nothing break residual, nothing and go rotten, without lopsided pea, average grain
Weight 0.15g；
(2) it sterilizes, the obtained pea of screening is immersed in bactericidal liquid and is sterilized 24 hours, with the steaming with the quality such as bactericidal liquid
Distilled water washed once, and obtain sterile pea；
The bactericidal liquid dosage is 5 times of pea quality；
The bactericidal liquid is by sodium hypochlorite: 4- phenylbenzimidazol derivative: water is 0.1:0.2:100 mixing in mass ratio
It forms；
The 4- phenylbenzimidazol derivative is 2- methyl 4-phenyl benzimidazole；
(3) miscegenation: chick-pea is mixed to sterile pea, 7 chick-pea is mixed in every 100 sterile peas, obtains hybrid
Son；
The chick-pea is sterilized before incorporation with method identical with sterile pea is obtained；
(4) vernalization: the N- acetyl-5-methoxytryptamine aqueous solution of 15 μm of ol/L of configuration, addition seed mixture, all kinds
Son is spread out, non-overlapping, and at 20 DEG C, relative humidity 80% after sprouting 7 days under conditions of being protected from light, removes chick-pea young shoot, obtains pea
Beans young shoot；
(5) it cultivates: pea young shoot is transferred in the seedlings nursing plate containing the culture substrate for being improved bud ratio, it is described to improve out
The culture substrate of bud rate with a thickness of 2cm, using common fluorescent lamp illumination, daily continuous illumination 12 hours is relatively wet at 25 DEG C
7 days are cultivated in the environment of degree 60% to get nutrition pea seedlings；
The preparation method of the culture substrate for improving bud ratio is by the phosphoric acid of formulation fertilizer containing nitrogen, 30% formulation fertilizer containing nitrogen quality
The magnesium sulfate of diammonium hydrogen and 25% formulation fertilizer containing nitrogen is added in the water of 1.1 times of formulation fertilizer containing nitrogen quality, heats 1 hour, adds at 60 DEG C
EnterZeolite, additional amount are the 80% of formulation fertilizer containing nitrogen quality, heat 2 hours at 70 DEG C, cross 300 meshes while hot, take solid,
At 170 DEG C dry 1 hour to get；
The formulation fertilizer containing nitrogen is
Embodiment 4
A kind of high yield pea seedlings implantation methods, plantation step are as follows:
(1) sieve kind: screening grain focuses on 0.12-0.20g, and free from insect pests, nothing break residual, nothing and go rotten, without lopsided pea, average grain
Weight 0.15g；
(2) it sterilizes, the obtained pea of screening is immersed in bactericidal liquid and is sterilized 24 hours, with the steaming with the quality such as bactericidal liquid
Distilled water washed once, and obtain sterile pea；
The bactericidal liquid dosage is 5 times of pea quality；
The bactericidal liquid is by sodium hypochlorite: 4- phenylbenzimidazol derivative: water is 0.1:0.2:100 mixing in mass ratio
It forms；
The 4- phenylbenzimidazol derivative is 2- methyl 4-phenyl benzimidazole；
(3) miscegenation: chick-pea is mixed to sterile pea, 7 chick-pea is mixed in every 100 sterile peas, obtains hybrid
Son；
The chick-pea is sterilized before incorporation with method identical with sterile pea is obtained；
(4) vernalization: the N- acetyl-5-methoxytryptamine aqueous solution of 15 μm of ol/L of configuration, addition seed mixture, all kinds
Son is spread out, non-overlapping, and at 20 DEG C, relative humidity 80% after sprouting 7 days under conditions of being protected from light, removes chick-pea young shoot, obtains pea
Beans young shoot；
(5) it cultivates: pea young shoot is transferred in the seedlings nursing plate containing the culture substrate for being improved bud ratio, it is described to improve out
The culture substrate of bud rate with a thickness of 2cm, using common fluorescent lamp illumination, daily continuous illumination 12 hours is relatively wet at 25 DEG C
7 days are cultivated in the environment of degree 60% to get nutrition pea seedlings；
The preparation method of the culture substrate for improving bud ratio is by the phosphoric acid of formulation fertilizer containing nitrogen, 30% formulation fertilizer containing nitrogen quality
The magnesium sulfate of diammonium hydrogen and 25% formulation fertilizer containing nitrogen is added in the water of 1.1 times of formulation fertilizer containing nitrogen quality, heats 1 hour, adds at 60 DEG C
Enter ca-montmorillonite, additional amount is the 80% of formulation fertilizer containing nitrogen quality, heats 2 hours at 70 DEG C, crosses 300 meshes while hot, is taken solid
Body, at 170 DEG C dry 1 hour to get；
The formulation fertilizer containing nitrogen is
Embodiment 5
A kind of high yield pea seedlings implantation methods, plantation step are as follows:
(1) sieve kind: screening grain focuses on 0.12-0.20g, and free from insect pests, nothing break residual, nothing and go rotten, without lopsided pea, average grain
Weight 0.15g；
(2) it sterilizes, the obtained pea of screening is immersed in bactericidal liquid and is sterilized 24 hours, with the steaming with the quality such as bactericidal liquid
Distilled water washed once, and obtain sterile pea；
The bactericidal liquid dosage is 5 times of pea quality；
The bactericidal liquid is by sodium hypochlorite: 4- phenylbenzimidazol derivative: water is 0.1:0.2:100 mixing in mass ratio
It forms；
The 4- phenylbenzimidazol derivative is 2- methyl 4-phenyl benzimidazole；
(3) miscegenation: chick-pea is mixed to sterile pea, 7 chick-pea is mixed in every 100 sterile peas, obtains hybrid
Son；
The chick-pea is sterilized before incorporation with method identical with sterile pea is obtained；
(4) vernalization: the N- acetyl-5-methoxytryptamine aqueous solution of 15 μm of ol/L of configuration, addition seed mixture, all kinds
Son is spread out, non-overlapping, and at 20 DEG C, relative humidity 80% after sprouting 7 days under conditions of being protected from light, removes chick-pea young shoot, obtains pea
Beans young shoot；
(5) it cultivates: pea young shoot is transferred in the seedlings nursing plate containing the culture substrate for being improved bud ratio, it is described to improve out
The culture substrate of bud rate with a thickness of 2cm, using common fluorescent lamp illumination, daily continuous illumination 12 hours is relatively wet at 25 DEG C
7 days are cultivated in the environment of degree 60% to get nutrition pea seedlings；
The preparation method of the culture substrate for improving bud ratio is by the phosphoric acid of formulation fertilizer containing nitrogen, 30% formulation fertilizer containing nitrogen quality
The magnesium sulfate of diammonium hydrogen and 25% formulation fertilizer containing nitrogen is added in the water of 1.1 times of formulation fertilizer containing nitrogen quality, heats 1 hour, adds at 60 DEG C
Entering alumino-silicate, additional amount is the 80% of formulation fertilizer containing nitrogen quality, it is heated at 70 DEG C 2 hours, crosses 300 meshes while hot, take solid,
At 170 DEG C dry 1 hour to get；
The formulation fertilizer containing nitrogen is
The alumino-silicate isThe mixing alumino-silicate that zeolite and ca-montmorillonite mass ratio are 2.5:1.
Embodiment 6
A kind of high yield pea seedlings implantation methods, plantation step are as follows:
(1) sieve kind: screening grain focuses on 0.12-0.20g, and free from insect pests, nothing break residual, nothing and go rotten, without lopsided pea, average grain
Weight 0.15g；
(2) it sterilizes, the obtained pea of screening is immersed in bactericidal liquid and is sterilized 24 hours, with the steaming with the quality such as bactericidal liquid
Distilled water washed once, and obtain sterile pea；
The bactericidal liquid dosage is 5 times of pea quality；
The bactericidal liquid is by sodium hypochlorite: 4- phenylbenzimidazol derivative: water is 0.1:0.2:100 mixing in mass ratio
It forms；
The 4- phenylbenzimidazol derivative structural formula are as follows:
(3) miscegenation: chick-pea is mixed to sterile pea, 7 chick-pea is mixed in every 100 sterile peas, obtains hybrid
Son；
The chick-pea is sterilized before incorporation with method identical with sterile pea is obtained；
(4) vernalization: the N- acetyl-5-methoxytryptamine aqueous solution of 15 μm of ol/L of configuration, addition seed mixture, all kinds
Son is spread out, non-overlapping, and at 20 DEG C, relative humidity 80% after sprouting 7 days under conditions of being protected from light, removes chick-pea young shoot, obtains pea
Beans young shoot；
(5) it cultivates: pea young shoot is transferred in the seedlings nursing plate containing the culture substrate for being improved bud ratio, it is described to improve out
The culture substrate of bud rate with a thickness of 2cm, using common fluorescent lamp illumination, daily continuous illumination 12 hours is relatively wet at 25 DEG C
7 days are cultivated in the environment of degree 60% to get nutrition pea seedlings；
The preparation method of the culture substrate for improving bud ratio is by the phosphoric acid of formulation fertilizer containing nitrogen, 30% formulation fertilizer containing nitrogen quality
The magnesium sulfate of diammonium hydrogen and 25% formulation fertilizer containing nitrogen is added in the water of 1.1 times of formulation fertilizer containing nitrogen quality, heats 1 hour, adds at 60 DEG C
Entering alumino-silicate, additional amount is the 80% of formulation fertilizer containing nitrogen quality, it is heated at 70 DEG C 2 hours, crosses 300 meshes while hot, take solid,
At 170 DEG C dry 1 hour to get；
The formulation fertilizer containing nitrogen is
The alumino-silicate isThe mixing alumino-silicate that zeolite and ca-montmorillonite mass ratio are 2.5:1.
Embodiment 7
A kind of high yield pea seedlings implantation methods, plantation step are as follows:
(1) sieve kind: screening grain focuses on 0.12-0.20g, and free from insect pests, nothing break residual, nothing and go rotten, without lopsided pea, average grain
Weight 0.15g；
(2) it sterilizes, the obtained pea of screening is immersed in bactericidal liquid and is sterilized 24 hours, with the steaming with the quality such as bactericidal liquid
Distilled water washed once, and obtain sterile pea；
The bactericidal liquid dosage is 5 times of pea quality；
The bactericidal liquid is by sodium hypochlorite: 4- phenylbenzimidazol derivative: water is 0.1:0.2:100 mixing in mass ratio
It forms；
The 4- phenylbenzimidazol derivative are as follows: 2- sulfonic group -4- phenylbenzimidazol；
(3) miscegenation: chick-pea is mixed to sterile pea, 7 chick-pea is mixed in every 100 sterile peas, obtains hybrid
Son；
The chick-pea is sterilized before incorporation with method identical with sterile pea is obtained；
(4) vernalization: the N- acetyl-5-methoxytryptamine aqueous solution of 15 μm of ol/L of configuration, addition seed mixture, all kinds
Son is spread out, non-overlapping, and at 20 DEG C, relative humidity 80% after sprouting 7 days under conditions of being protected from light, removes chick-pea young shoot, obtains pea
Beans young shoot；
(5) it cultivates: pea young shoot is transferred in the seedlings nursing plate containing the culture substrate for being improved bud ratio, it is described to improve out
The culture substrate of bud rate with a thickness of 2cm, using common fluorescent lamp illumination, daily continuous illumination 12 hours is relatively wet at 25 DEG C
7 days are cultivated in the environment of degree 60% to get nutrition pea seedlings；
The preparation method of the culture substrate for improving bud ratio is by the phosphoric acid of formulation fertilizer containing nitrogen, 30% formulation fertilizer containing nitrogen quality
The magnesium sulfate of diammonium hydrogen and 25% formulation fertilizer containing nitrogen is added in the water of 1.1 times of formulation fertilizer containing nitrogen quality, heats 1 hour, adds at 60 DEG C
Entering alumino-silicate, additional amount is the 80% of formulation fertilizer containing nitrogen quality, it is heated at 70 DEG C 2 hours, crosses 300 meshes while hot, take solid,
At 170 DEG C dry 1 hour to get；
The formulation fertilizer containing nitrogen is
The alumino-silicate isThe mixing alumino-silicate that zeolite and ca-montmorillonite mass ratio are 2.5:1.
Test case 1
Bud ratio statistics
1000 pea seedlings of each plantation, statistics are planted using the method for culture substrate of the invention to according to embodiment 1-7
Three batches, five batches and seven batches of bud ratio.Still pressed old terms culture 5 days after pea seedlings harvesting, 5 days endogenous budding height less than 5cm or
It does not sprout to be considered as and not sprout, and remove.Statistics radix is 1000 when calculating bud ratio.
Statistical result is as shown in table 1.
1 bud ratio of table
| Three batches of bud ratios (%) | Five batches of bud ratios (%) | Seven batches of bud ratios (%) | |
| Embodiment 1 | 95.4 | 65.4 | 42.6 |
| Embodiment 2 | 96.3 | 66.2 | 45.9 |
| Embodiment 3 | 97.0 | 73.9 | 55.0 |
| Embodiment 4 | 97.7 | 73.5 | 54.4 |
| Embodiment 5 | 98.8 | 80.7 | 68.4 |
| Embodiment 6 | 99.3 | 82.5 | 71.5 |
| Embodiment 7 | 99.4 | 84.3 | 79.5 |
As seen from the above table, pea seedlings implantation methods provided by the invention have very high bud ratio, traditional implantation methods one
It can only go out as soon as batch, relatively advanced method generally also goes out three batches in the prior art, but method provided by the invention is universal
It can go out to five batches, most 77.5% can go out to seven batches, far beyond the prior art.
Test case 2
The measurement of pea seedlings total phenol content
The pea seedlings first planting total phenol content that embodiment 1-7 is cultivated using culture substrate of the invention is measured, measuring method is
Folin-Ciocalteu colorimetric method.
Test result is as shown in table 2.
2 total phenol content of table
| Total phenol content (mg/g) | |
| Embodiment 1 | 686.4 |
| Embodiment 2 | 692.3 |
| Embodiment 3 | 725.3 |
| Embodiment 4 | 739.0 |
| Embodiment 5 | 794.8 |
| Embodiment 6 | 812.9 |
| Embodiment 7 | 846.2 |
As seen from the above table, the pea seedlings that the pea seedlings implantation methods of offer culture substrate are planted through the invention has
Good nutritive value, total phenol content is more than 650mg/g.The implantation methods wherein especially provided with embodiment 7 are best, obtain
First planting pea seedlings total phenol content can be up to 846.2mg/g.
Claims (7)
1. it is a kind of improve bud ratio culture substrate preparation method, which comprises the steps of: by formulation fertilizer containing nitrogen,
Inorganic phosphate and magnesium sulfate are added to the water, and heat 1-2 hours at 50-60 DEG C, alumino-silicate are added, at 60-80 DEG C
Heating 2-3 hour, filter while hot, take solid dry to get.
2. improving the preparation method of the culture substrate of bud ratio as described in claim 1, which is characterized in that including walking as follows
It is rapid: by formulation fertilizer containing nitrogen, to account for the inorganic phosphate of formulation fertilizer containing nitrogen quality 20-50% and account for the sulfuric acid of formulation fertilizer containing nitrogen quality 20-50%
Magnesium is added in the water of 1-1.2 times of formulation fertilizer containing nitrogen quality, is heated 1-2 hours at 50-60 DEG C, and alumino-silicate, additional amount is added
It for the 70-90% of formulation fertilizer containing nitrogen quality, heats 2-3 hours at 60-80 DEG C, filters while hot, take solid, done at 150-180 DEG C
Dry 0.5-2 hours to get.
3. improving the preparation method of the culture substrate of bud ratio as claimed in claim 1 or 2, it is characterised in that: described nitrogenous
Fertilizer is amidino groups urea derivative.
4. improving the preparation method of the culture substrate of bud ratio as claimed in claim 3, it is characterised in that: the dicyandiamidines spreads out
The structural formula of biology is as follows:
5. improving the preparation method of the culture substrate of bud ratio as claimed in claim 1 or 2, it is characterised in that: described inorganic
Phosphate is ammonium phosphate or diammonium hydrogen phosphate.
6. improving the preparation method of the culture substrate of bud ratio as claimed in claim 1 or 2, it is characterised in that: the sial
Hydrochlorate is the mixture of montmorillonite or zeolite or both arbitrary proportion.
7. a kind of culture substrate for improving bud ratio, using the culture medium for improving bud ratio as described in any one of claim 1~6
The preparation method of matter is prepared.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103907517A (en) * | 2013-01-05 | 2014-07-09 | 赵百华 | Pea seedling cultivation technology |
| CN108033838A (en) * | 2017-12-11 | 2018-05-15 | 佛山市田森温室科技有限公司 | The preparation method of potato culture medium |
| CN108124729A (en) * | 2018-01-23 | 2018-06-08 | 合肥百绿盛农业科技有限公司 | A kind of preparation method of seedling-nourishing matrix |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103907517A (en) * | 2013-01-05 | 2014-07-09 | 赵百华 | Pea seedling cultivation technology |
| CN108033838A (en) * | 2017-12-11 | 2018-05-15 | 佛山市田森温室科技有限公司 | The preparation method of potato culture medium |
| CN108124729A (en) * | 2018-01-23 | 2018-06-08 | 合肥百绿盛农业科技有限公司 | A kind of preparation method of seedling-nourishing matrix |
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| 开恳等: "沸石吸附-生物再生活性污泥工艺对比试验研究 ", 《江苏环境科技》 * |
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