CN109548561A - A kind of pilose antler mushroom culture method - Google Patents
A kind of pilose antler mushroom culture method Download PDFInfo
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- CN109548561A CN109548561A CN201811595025.5A CN201811595025A CN109548561A CN 109548561 A CN109548561 A CN 109548561A CN 201811595025 A CN201811595025 A CN 201811595025A CN 109548561 A CN109548561 A CN 109548561A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/50—Inoculation of spawn
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- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
Abstract
This application involves a kind of pilose antler mushroom culture methods, pilose antler mushroom has finally been harvested by bottling, inoculation, culture, mycelium stimulation, flower bud, mushroom growing, harvesting step, pilose antler mushroom new strains per unit area yield can be made to reach 200 grams, production cycle foreshortens to 76 days, contamination of products rate is down to 0.1%, and biological conversion efficiency fluctuating range is below 6%.With yield height, pilose antler mushroom production cycle fast advantage.
Description
Technical field
The application belongs to mushroom production technical field, more particularly, to a kind of pilose antler mushroom culture method.
Background technique
Pilose antler mushroom, also known as Lyophyllum decastes are internationally famous delicious edible mushrooms.There is the title of " fried chicken mushroom " in Europe.Deer
Fine and soft mushroom is fleshy hypertrophy, and tasting, A sweety scent assails the nostrils, delicious flavour, mouthfeel chewiness.Present pilose antler mushroom industrialized cultivation is mainly manifested in
The following aspects, first is that it is low when high when pollution rate, occur facing total crop failure state in frequency in continuous 20 days when serious, loss reaches hundreds of
Wan Yuan；Second is that yield height is uneven, and same raw material and kind, in same cultivation place, under same control system, difference batch
Secondary pollutant efficiency fluctuation is more than 50%；Third is that unstable quality or even continuous two production batch quality differences are aobvious, while dirty
Dye rate and yield and quality show as negative correlation, i.e. pollution rate is higher, and yield is lower, and quality is also poorer；Four, batch production is main
The new edible fungus industrial category of the products such as centralized production needle mushroom, high added value is weeded out the old and bring forth the new slowly, obvious with Japanese gap.Deer
The production of fine and soft mushroom is the important component of mushroom industry, is Developing Urban Agriculture, ecological industry, makes the rural economy flourish and promote agriculture
The important content of people's increase wealth.However, due to lacking high-quality pilose antler mushroom kind with independent intellectual property rights, the pilose antler of our province
Mushroom production occurs being easy the disadvantages of pollution, yield rate is low, production efficiency is low, the consumption energy is high in various degree, significantly reduces
The economic benefit of enterprise can not embody the greatest benefit of pilose antler mushroom, and these problems, which have become, restricts our province pilose antler mushroom hair
The important link of exhibition.
Summary of the invention
The technical problem to be solved by the present invention is to solve deficiency in the prior art, to provide a kind of production efficiency
High pilose antler mushroom culture method.
The technical solution adopted by the present invention to solve the technical problems is:
A kind of pilose antler mushroom culture method,
S1: bottling: matrix sawdust is put into bottle, is sterilized；
S2: inoculation: pilose antler mushroom is inoculated at 30 DEG C of bottle temperature hereinafter, being fitted into bottle in gnotobasis；
S3: culture: the aseptic bottle after inoculation is placed in 23-25 DEG C of constant temperature in dark surrounds, bottle is expired in culture to mycelia；
S4: mycelium stimulation: mycelia is further cultured for 7-10 days after expiring bottle, reaches physiological maturity, at this time mycelium stimulation；
S5: flower bud: enter downwards to plough under bottleneck one empty basket after mycelium stimulation, be conducive to mycelia restoration ecosystem, air humidity 90%-
95%, 12-15 DEG C of temperature, proper ventilation；After mycelia restoration ecosystem, humidity drops to 80%-85%, forms psychrometric difference, same to time control
Illumination processed is 500-800Lx, and CO2 concentration is 0.1% hereinafter, culture forms mushroom flower bud；
S6: mushroom growing: mushroom flower bud turns over basket after being formed again, makes bottleneck mushroom growing upward, and temperature control at this time is at 15-17 DEG C, humidity control
System is in 90%-95%；
S7: harvesting: mushroom lid is unfolded substantially, is harvested when spore does not launch.
Preferably, pilose antler mushroom culture method of the invention, the formula of the matrix are 100 mass parts sawdusts, 25-30 mass
Part corncob, 22-28 mass parts wheat bran, 4-6 mass parts soybean skin, 2-4 mass parts Soybean Meal, 6-7 mass parts corn flour, 0.8-
0.9 mass parts calcium hydroxide, 0.9-1.1 mass parts calcium carbonate.
Preferably, pilose antler mushroom culture method of the invention in S3 step, keeps in bottle CO2 concentration by normal air
Amount 0.03% gradually rises to 0.22%.
Preferably, pilose antler mushroom culture method of the invention removes bottleneck 1-1.5cm thickness aging mycelia when mycelium stimulation.
Preferably, pilose antler mushroom culture method of the invention co-cultures 30-35 days in S3 step.
Preferably, pilose antler mushroom culture method of the invention, in S5, the time for forming mushroom flower bud is 7-10 days.
Preferably, pilose antler mushroom culture method of the invention,
The breeding method carries out in mushroom house；
Mushroom house is divided into bacteria-producing room, flower bud room and mushroom growing room, and culture and mycelium stimulation carry out in bacteria-producing room, and flower bud is in flower bud room
It carries out, mushroom growing carries out in mushroom growing room, mushroom house wide 3.5M, long 9M, high 3.5M, and the door of bacteria-producing room, flower bud room and mushroom growing room is unified
It is opened to corridor, the wide 2M in corridor corridor, the wall spraying polyethylene foam insulation of mushroom house, mushroom frame biserial is to arrangement, surrounding and centre
There are passageway, 7 layers of bed of bacteria-producing room mushroom, layer is away from 0.35M, and the mushroom bed of flower bud room and mushroom growing room is 5 floor, and layer is away from 0.45M, bottom mushroom bed
It is 0.25M away from ground.
Preferably, pilose antler mushroom culture method of the invention is each equipped with refrigeration in bacteria-producing room, flower bud room and mushroom growing room and sets
It is standby, ventilation equipment, spraying apparatus and light irradiation apparatus.
The beneficial effects of the present invention are:
Pilose antler mushroom culture method of the invention can make pilose antler mushroom new strains per unit area yield reach 200 grams, and the production cycle foreshortens to
76 days, contamination of products rate was down to 0.1%, and biological conversion efficiency fluctuating range is below 6%.With yield height, pilose antler mushroom is raw
Produce period fast advantage.
Specific embodiment
It should be noted that in the absence of conflict, the features in the embodiments and the embodiments of the present application can phase
Mutually combination.
Embodiment 1
The present embodiment provides a kind of pilose antler mushroom culture method,
S1: bottling: matrix being put into bottle, is sterilized, and the formula of the matrix is 100 mass parts sawdusts, 25 mass
Part corncob, 22 mass parts wheat brans, 6 mass parts soybean skins, 2 mass parts Soybean Meaies, 7 mass parts corn flour, 0.8 mass parts hydrogen-oxygen
Change calcium, 1.1 mass parts calcium carbonate；
S2: inoculation: pilose antler mushroom is inoculated at 30 DEG C of bottle temperature hereinafter, being fitted into bottle in gnotobasis；
S3: culture: the aseptic bottle after inoculation is placed in 23 DEG C of constant temperature in dark surrounds, keeps CO in bottle2Concentration is by normal
Amount 0.03% in air gradually rises to 0.22%, higher concentration CO2Mycelia can be stimulated to grow, so changing on a small quantity during culture
Gas, cultivating 30-35 days culture to mycelia expires bottle；
S4: mycelium stimulation: mycelia is further cultured for 7 days after expiring bottle, reaches physiological maturity, at this time mycelium stimulation, and it is thick old to remove bottleneck 1cm
Change mycelia；
S5: flower bud: entering downwards to plough under bottleneck one empty basket after mycelium stimulation, is conducive to mycelia restoration ecosystem, air humidity 90%,
12 DEG C of temperature, proper ventilation；After mycelia restoration ecosystem, humidity drops to 80%%, forms psychrometric difference, while controlling illumination and being
500Lx, CO2 concentration are 0.1% hereinafter, 7-10 days formation mushroom flower buds of culture；
S6: mushroom growing: mushroom flower bud turns over basket after being formed again, makes bottleneck mushroom growing upward, and at 15 DEG C, humid control exists for temperature control at this time
90%；
S7: harvesting: mushroom lid is unfolded substantially, is harvested when spore does not launch.
Embodiment 2
The present embodiment provides a kind of pilose antler mushroom culture method,
S1: bottling: matrix being put into bottle, is sterilized, and the formula of the matrix is 100 mass parts sawdusts, 28 mass
Part corncob, 25 mass parts wheat brans, 5 mass parts soybean skins, 3 mass parts Soybean Meaies, 6.5 mass parts corn flour, 0.85 mass parts
Calcium hydroxide, 1 mass parts calcium carbonate；
S2: inoculation: pilose antler mushroom is inoculated at 30 DEG C of bottle temperature hereinafter, being fitted into bottle in gnotobasis；
S3: culture: the aseptic bottle after inoculation is placed in 24 DEG C of constant temperature in dark surrounds, keeps CO in bottle2Concentration is by normal
Amount 0.03% in air gradually rises to 0.22%, higher concentration CO2Mycelia can be stimulated to grow, so changing on a small quantity during culture
Gas, cultivating 32 days culture to mycelia expires bottle；
S4: mycelium stimulation: mycelia is further cultured for 8 days after expiring bottle, reaches physiological maturity, at this time mycelium stimulation, and it is thick to remove bottleneck 1.2cm
Aging mycelia；
S5: flower bud: entering downwards to plough under bottleneck one empty basket after mycelium stimulation, is conducive to mycelia restoration ecosystem, air humidity 92%,
13 DEG C of temperature, proper ventilation；After mycelia restoration ecosystem, humidity drops to 82%, forms psychrometric difference, while controlling illumination is 600Lx,
CO2Concentration is 0.1% hereinafter, 7-10 days formation mushroom flower buds of culture；
S6: mushroom growing: mushroom flower bud turns over basket after being formed again, makes bottleneck mushroom growing upward, and at 16 DEG C, humid control exists for temperature control at this time
92%-；
S7: harvesting: mushroom lid is unfolded substantially, is harvested when spore does not launch.
Embodiment 3
The present embodiment provides a kind of pilose antler mushroom culture method,
S1: bottling: matrix being put into bottle, is sterilized, and the formula of the matrix is 100 mass parts sawdusts, 30 mass
Part corncob, 28 mass parts wheat brans, 4 mass parts soybean skins, 4 mass parts Soybean Meaies, 6 mass parts corn flour, 0.9 mass parts hydrogen-oxygen
Change calcium, 0.9 mass parts calcium carbonate；
S2: inoculation: pilose antler mushroom is inoculated at 30 DEG C of bottle temperature hereinafter, being fitted into bottle in gnotobasis；
S3: culture: the aseptic bottle after inoculation is placed in 25 DEG C of constant temperature in dark surrounds, keeps CO in bottle2Concentration is by normal
Amount 0.03% in air gradually rises to 0.22%, higher concentration CO2Mycelia can be stimulated to grow, so changing on a small quantity during culture
Gas, cultivating 35 days culture to mycelia expires bottle；
S4: mycelium stimulation: mycelia is further cultured for 10 days after expiring bottle, reaches physiological maturity, at this time mycelium stimulation, removes bottleneck 1.5cm
Thick aging mycelia；
S5: flower bud: entering downwards to plough under bottleneck one empty basket after mycelium stimulation, is conducive to mycelia restoration ecosystem, air humidity 95%,
13 DEG C of temperature, proper ventilation；After mycelia restoration ecosystem, humidity drops to 85%, forms psychrometric difference, while controlling illumination is 800Lx,
CO2Concentration is 0.1% hereinafter, 7-10 days formation mushroom flower buds of culture；
S6: mushroom growing: mushroom flower bud turns over basket after being formed again, makes bottleneck mushroom growing upward, and at 17 DEG C, humid control exists for temperature control at this time
95%；
S7: harvesting: mushroom lid is unfolded substantially, is harvested when spore does not launch.
In embodiment 1-3, breeding method carries out in mushroom house；
Mushroom house is divided into bacteria-producing room, flower bud room and mushroom growing room, and culture and mycelium stimulation carry out in bacteria-producing room, and flower bud is in flower bud room
It carries out, mushroom growing carries out in mushroom growing room, mushroom house wide 3.5M, long 9M, high 3.5M, and the door of bacteria-producing room, flower bud room and mushroom growing room is unified
It is opened to corridor, the wide 2M in corridor corridor, the wall spraying polyethylene foam insulation of mushroom house, mushroom frame biserial is to arrangement, surrounding and centre
There are passageway, 7 layers of bed of bacteria-producing room mushroom, layer is away from 0.35M, and the mushroom bed of flower bud room and mushroom growing room is 5 floor, and layer is away from 0.45M, bottom mushroom bed
It is 0.25M away from ground.Refrigeration equipment, ventilation equipment, spraying apparatus and illumination are each equipped in bacteria-producing room, flower bud room and mushroom growing room
Equipment.Each room is equipped with the refrigeration machine and 1 40M of 1 5HP2Furred ceiling air-cooler；Or 2 Room be equipped with 1 8HP refrigeration unit and 2
Platform 40M2Furred ceiling air-cooler.On the ceiling of flower bud room and mushroom growing room and longitudinal two pile walls respectively install 2 40W fluorescent lamps.Respectively
1 45W axis galvanic electricity fan is installed in room, and fresh air squeezes into mushroom house via surge chamber, exhaust gas from another exhaust outlet through surge chamber every
Layer discharge.
New strains per unit area yield is set to reach 200 grams, the production cycle foreshortens to 76 days, and contamination of products rate is down to 0.1%, and biology turns
Change efficiency fluctuation amplitude below 6%.
It is enlightenment with the above-mentioned desirable embodiment according to the application, through the above description, relevant staff is complete
Full various changes and amendments can be carried out in the range of without departing from this item application technical idea.The technology of this item application
Property range is not limited to the contents of the specification, it is necessary to which the technical scope thereof is determined according to the scope of the claim.
Claims (8)
1. a kind of pilose antler mushroom culture method, which is characterized in that
S1: bottling: matrix is put into bottle, is sterilized；
S2: inoculation: pilose antler mushroom is inoculated at 30 DEG C of bottle temperature hereinafter, being fitted into bottle in gnotobasis；
S3: culture: the aseptic bottle after inoculation is placed in 23-25 DEG C of constant temperature in dark surrounds, bottle is expired in culture to mycelia；
S4: mycelium stimulation: mycelia is further cultured for 7-10 days after expiring bottle, reaches physiological maturity, at this time mycelium stimulation；
S5: flower bud: enter downwards to plough under bottleneck one empty basket after mycelium stimulation, be conducive to mycelia restoration ecosystem, air humidity 90%-
95%, 12-15 DEG C of temperature, proper ventilation；After mycelia restoration ecosystem, humidity drops to 80%-85%, forms psychrometric difference, same to time control
Illumination processed is 500-800Lx, CO2Concentration is 0.1% hereinafter, culture forms mushroom flower bud；
S6: mushroom growing: mushroom flower bud turns over basket after being formed again, makes bottleneck mushroom growing upward, and at 15-17 DEG C, humid control exists for temperature control at this time
90%-95%；
S7: harvesting: mushroom lid is unfolded substantially, is harvested when spore does not launch.
2. pilose antler mushroom culture method according to claim 1, which is characterized in that the formula of the matrix is 100 mass parts
Sawdust, 25-30 mass parts corncob, 22-28 mass parts wheat bran, 4-6 mass parts soybean skin, 2-4 mass parts Soybean Meal, 6-7 matter
Measure part corn flour, 0.8-0.9 mass parts calcium hydroxide, 0.9-1.1 mass parts calcium carbonate.
3. pilose antler mushroom culture method according to claim 1 or 2, which is characterized in that in S3 step, keep CO in bottle2Concentration
0.22% is gradually risen to by the amount in normal air 0.03%.
4. pilose antler mushroom culture method according to claim 1-3, which is characterized in that remove bottleneck 1- when mycelium stimulation
1.5cm thickness aging mycelia.
5. pilose antler mushroom culture method according to claim 1-4, which is characterized in that co-culture 30- in S3 step
35 days.
6. pilose antler mushroom culture method according to claim 1-5, which is characterized in that in S5, formed mushroom flower bud when
Between be 7-10 days.
7. pilose antler mushroom culture method according to claim 1-6, which is characterized in that
The breeding method carries out in mushroom house；
Mushroom house is divided into bacteria-producing room, flower bud room and mushroom growing room, and culture and mycelium stimulation carry out in bacteria-producing room, flower bud in flower bud room into
Row, mushroom growing carry out in mushroom growing room, and the wide 3.5M of mushroom house, the door of long 9M, high 3.5M, bacteria-producing room, flower bud room and mushroom growing room is uniformly opened
To corridor, the wide 2M in corridor corridor, the wall spraying polyethylene foam insulation of mushroom house, mushroom frame biserial stays to arrangement, surrounding and centre
Have a passageway, 7 layers of bed of bacteria-producing room mushroom, for layer away from 0.35M, the mushroom bed of flower bud room and mushroom growing room is 5 floor, layer away from 0.45M, bottom mushroom bed away from
Ground is 0.25M.
8. pilose antler mushroom culture method according to claim 1-7, which is characterized in that bacteria-producing room, flower bud room and educate
Refrigeration equipment, ventilation equipment, spraying apparatus and light irradiation apparatus are each equipped in mushroom room.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811595025.5A CN109548561A (en) | 2018-12-25 | 2018-12-25 | A kind of pilose antler mushroom culture method |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111279976A (en) * | 2020-04-14 | 2020-06-16 | 安远县天华现代农业有限责任公司 | Velvet antler mushroom culture medium and preparation method and application thereof |
| CN111296179A (en) * | 2020-03-31 | 2020-06-19 | 云南强丰农业科技有限公司 | Velvet antler mushroom culture medium and production method thereof |
| CN111771616A (en) * | 2020-08-18 | 2020-10-16 | 湖南和平生物科技有限公司 | Industrialized cultivation method of velvet antler mushroom |
| CN113711840A (en) * | 2021-08-19 | 2021-11-30 | 江苏江南生物科技有限公司 | Intensive and large-scale cultivation method for velvet antler mushroom |
| CN116171797A (en) * | 2023-04-04 | 2023-05-30 | 福建恒绿生物科技有限公司 | Cultivation method of velvet mushrooms |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111296179A (en) * | 2020-03-31 | 2020-06-19 | 云南强丰农业科技有限公司 | Velvet antler mushroom culture medium and production method thereof |
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| CN111771616A (en) * | 2020-08-18 | 2020-10-16 | 湖南和平生物科技有限公司 | Industrialized cultivation method of velvet antler mushroom |
| CN113711840A (en) * | 2021-08-19 | 2021-11-30 | 江苏江南生物科技有限公司 | Intensive and large-scale cultivation method for velvet antler mushroom |
| CN116171797A (en) * | 2023-04-04 | 2023-05-30 | 福建恒绿生物科技有限公司 | Cultivation method of velvet mushrooms |
| CN116171797B (en) * | 2023-04-04 | 2024-06-07 | 福建恒绿生物科技有限公司 | Cultivation method of velvet mushrooms |
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Application publication date: 20190402 |