CN109490432A - 一种鉴别蜂蜜中是否掺入油菜蜜的方法 - Google Patents
一种鉴别蜂蜜中是否掺入油菜蜜的方法 Download PDFInfo
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- CN109490432A CN109490432A CN201811228425.2A CN201811228425A CN109490432A CN 109490432 A CN109490432 A CN 109490432A CN 201811228425 A CN201811228425 A CN 201811228425A CN 109490432 A CN109490432 A CN 109490432A
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- 235000012907 honey Nutrition 0.000 title claims abstract description 191
- 238000000034 method Methods 0.000 title claims abstract description 40
- 238000001514 detection method Methods 0.000 claims abstract description 22
- 239000012071 phase Substances 0.000 claims description 58
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 57
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 36
- 238000004128 high performance liquid chromatography Methods 0.000 claims description 31
- JLIDBLDQVAYHNE-YKALOCIXSA-N (+)-Abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\[C@@]1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-YKALOCIXSA-N 0.000 claims description 23
- 239000012488 sample solution Substances 0.000 claims description 23
- 239000003480 eluent Substances 0.000 claims description 22
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 239000004615 ingredient Substances 0.000 claims description 13
- 238000000605 extraction Methods 0.000 claims description 12
- 239000000126 substance Substances 0.000 claims description 12
- 235000010643 Leucaena leucocephala Nutrition 0.000 claims description 10
- 238000002414 normal-phase solid-phase extraction Methods 0.000 claims description 10
- 238000005349 anion exchange Methods 0.000 claims description 9
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims description 8
- YNCRBFODOPHHAO-YUELXQCFSA-N Phaseic acid Natural products CC(=CC(=O)O)C=C[C@@H]1[C@@]2(C)CO[C@@]1(C)CC(=O)C2 YNCRBFODOPHHAO-YUELXQCFSA-N 0.000 claims description 8
- 238000004090 dissolution Methods 0.000 claims description 8
- 235000019253 formic acid Nutrition 0.000 claims description 8
- IZGYIFFQBZWOLJ-UHFFFAOYSA-N neophaseic acid Natural products C1C(=O)CC2(C)OCC1(C)C2(O)C=CC(C)=CC(O)=O IZGYIFFQBZWOLJ-UHFFFAOYSA-N 0.000 claims description 8
- IZGYIFFQBZWOLJ-CKAACLRMSA-N phaseic acid Chemical compound C1C(=O)C[C@@]2(C)OC[C@]1(C)[C@@]2(O)C=CC(/C)=C\C(O)=O IZGYIFFQBZWOLJ-CKAACLRMSA-N 0.000 claims description 8
- JMSVCTWVEWCHDZ-UHFFFAOYSA-N syringic acid Chemical compound COC1=CC(C(O)=O)=CC(OC)=C1O JMSVCTWVEWCHDZ-UHFFFAOYSA-N 0.000 claims description 8
- 244000166124 Eucalyptus globulus Species 0.000 claims description 6
- 238000011068 loading method Methods 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 150000004702 methyl esters Chemical class 0.000 claims description 4
- YIBXWXOYFGZLRU-UHFFFAOYSA-N syringic aldehyde Natural products CC12CCC(C3(CCC(=O)C(C)(C)C3CC=3)C)C=3C1(C)CCC2C1COC(C)(C)C(O)C(O)C1 YIBXWXOYFGZLRU-UHFFFAOYSA-N 0.000 claims description 4
- 240000006909 Tilia x europaea Species 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 238000000825 ultraviolet detection Methods 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 2
- -1 anti- Chemical compound 0.000 claims description 2
- 240000007472 Leucaena leucocephala Species 0.000 claims 1
- 238000011088 calibration curve Methods 0.000 claims 1
- 235000013305 food Nutrition 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 abstract description 2
- 229910021642 ultra pure water Inorganic materials 0.000 description 14
- 239000012498 ultrapure water Substances 0.000 description 14
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 12
- 241000220479 Acacia Species 0.000 description 9
- 238000001035 drying Methods 0.000 description 6
- 238000010812 external standard method Methods 0.000 description 6
- 229910052757 nitrogen Inorganic materials 0.000 description 6
- 238000004445 quantitative analysis Methods 0.000 description 6
- 239000000523 sample Substances 0.000 description 6
- 239000006228 supernatant Substances 0.000 description 6
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 5
- ZCHPKWUIAASXPV-UHFFFAOYSA-N acetic acid;methanol Chemical compound OC.CC(O)=O ZCHPKWUIAASXPV-UHFFFAOYSA-N 0.000 description 5
- 239000000908 ammonium hydroxide Substances 0.000 description 5
- 238000007689 inspection Methods 0.000 description 5
- 239000012528 membrane Substances 0.000 description 5
- 238000010348 incorporation Methods 0.000 description 4
- 230000004913 activation Effects 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 238000010828 elution Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000011017 operating method Methods 0.000 description 3
- 238000004451 qualitative analysis Methods 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- PMKQSEYPLQIEAY-YWNRKNDBSA-N Phaseolic acid Chemical compound OC(=O)C[C@@H](C(O)=O)OC(=O)\C=C\C1=CC=C(O)C(O)=C1 PMKQSEYPLQIEAY-YWNRKNDBSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- FCRACOPGPMPSHN-UHFFFAOYSA-N desoxyabscisic acid Natural products OC(=O)C=C(C)C=CC1C(C)=CC(=O)CC1(C)C FCRACOPGPMPSHN-UHFFFAOYSA-N 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000013441 quality evaluation Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 210000000697 sensory organ Anatomy 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 244000020518 Carthamus tinctorius Species 0.000 description 1
- 235000003255 Carthamus tinctorius Nutrition 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 244000223014 Syzygium aromaticum Species 0.000 description 1
- 235000016639 Syzygium aromaticum Nutrition 0.000 description 1
- 240000007591 Tilia tomentosa Species 0.000 description 1
- 150000003529 abscisic acid derivatives Chemical class 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- PMKQSEYPLQIEAY-SNVBAGLBSA-N caffeoyl malate Natural products OC(=O)C[C@@H](OC(=O)C=Cc1ccc(O)c(O)c1)C(O)=O PMKQSEYPLQIEAY-SNVBAGLBSA-N 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000011140 membrane chromatography Methods 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000012372 quality testing Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Jellies, Jams, And Syrups (AREA)
Abstract
Description
Claims (10)
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CN201811228425.2A CN109490432B (zh) | 2018-10-22 | 2018-10-22 | 一种鉴别蜂蜜中是否掺入油菜蜜的方法 |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109946406A (zh) * | 2019-04-15 | 2019-06-28 | 许昌学院 | 一种鉴别椴树蜂蜜真假方法 |
CN111896649A (zh) * | 2020-08-03 | 2020-11-06 | 西北大学 | 一种成熟蜂蜜和未成熟蜂蜜的鉴别方法 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010082845A1 (en) * | 2008-12-24 | 2010-07-22 | Comvita New Zealand Limited | Honey analysis |
CN103592395A (zh) * | 2013-12-06 | 2014-02-19 | 西北大学 | 洋槐蜜中掺有油菜蜜的鉴别方法 |
CN105548233A (zh) * | 2015-10-30 | 2016-05-04 | 中国科学院武汉物理与数学研究所 | 一种基于氢核磁共振鉴别洋槐蜜和油菜蜜的方法 |
CN107449837A (zh) * | 2017-01-09 | 2017-12-08 | 北京工商大学 | 利用非糖特征物质鉴别蜂蜜与糖浆 |
CN108614000A (zh) * | 2018-04-13 | 2018-10-02 | 江苏出入境检验检疫局动植物与食品检测中心 | 基于simca模型的麦卢卡蜂蜜鉴别方法 |
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2018
- 2018-10-22 CN CN201811228425.2A patent/CN109490432B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010082845A1 (en) * | 2008-12-24 | 2010-07-22 | Comvita New Zealand Limited | Honey analysis |
CN103592395A (zh) * | 2013-12-06 | 2014-02-19 | 西北大学 | 洋槐蜜中掺有油菜蜜的鉴别方法 |
CN105548233A (zh) * | 2015-10-30 | 2016-05-04 | 中国科学院武汉物理与数学研究所 | 一种基于氢核磁共振鉴别洋槐蜜和油菜蜜的方法 |
CN107449837A (zh) * | 2017-01-09 | 2017-12-08 | 北京工商大学 | 利用非糖特征物质鉴别蜂蜜与糖浆 |
CN108614000A (zh) * | 2018-04-13 | 2018-10-02 | 江苏出入境检验检疫局动植物与食品检测中心 | 基于simca模型的麦卢卡蜂蜜鉴别方法 |
Non-Patent Citations (5)
Title |
---|
CHUNLI SUN ET AL.: "Phenolics and abscisic acid identified in acacia honey comparing different SPE cartridges coupled with HPLC-PDA", 《JOURNAL OF FOOD COMPOSITION AND ANALYSIS》 * |
孙春丽 等: "市售洋槐蜜多酚类成分及其抗氧化活性研究", 《江苏农业科学》 * |
岳锦萍 等: "蜂蜜的主要成分及其鉴别技术", 《食品安全质量检测学报》 * |
张文文 等: "麦卢卡蜂蜜快速鉴别技术的开发与应用", 《农产品质量与安全》 * |
赵宏玉: "椴树蜜、荞麦蜜及赞比亚蜜中多酚类成分的研究", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109946406A (zh) * | 2019-04-15 | 2019-06-28 | 许昌学院 | 一种鉴别椴树蜂蜜真假方法 |
CN111896649A (zh) * | 2020-08-03 | 2020-11-06 | 西北大学 | 一种成熟蜂蜜和未成熟蜂蜜的鉴别方法 |
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Effective date of registration: 20230203 Address after: 831111 No. 612 Tuanjie Road, Junhu Farm Town, Changji City, Changji Hui Autonomous Prefecture, Xinjiang Uygur Autonomous Region Patentee after: Xinjiang xinxingyuan Agriculture Co.,Ltd. Address before: 1 xiangshanbeigou, Haidian District, Beijing Patentee before: INSTITUTE OF APICULTURAL RESEARCH CHINESE ACADEMY OF AGRICULTURAL SCIENCES |
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Effective date of registration: 20231007 Address after: No. 20 Malan Lane, Junzhureke Village, Jilande Pasture, Yumin County, Tacheng Prefecture, Xinjiang Uygur Autonomous Region, 834804 Patentee after: Xinjiang Silk Road Herbal Medicine Co.,Ltd. Address before: 831111 No. 612 Tuanjie Road, Junhu Farm Town, Changji City, Changji Hui Autonomous Prefecture, Xinjiang Uygur Autonomous Region Patentee before: Xinjiang xinxingyuan Agriculture Co.,Ltd. |