CN109475663A - 一种脱细胞猪角膜的制备方法及其脱细胞板层角膜和用法 - Google Patents

一种脱细胞猪角膜的制备方法及其脱细胞板层角膜和用法 Download PDF

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CN109475663A
CN109475663A CN201680077703.1A CN201680077703A CN109475663A CN 109475663 A CN109475663 A CN 109475663A CN 201680077703 A CN201680077703 A CN 201680077703A CN 109475663 A CN109475663 A CN 109475663A
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cornea
preparation
takes
plate layer
pig
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CN109475663B (zh
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李志寒
李洁
詹晓亮
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Xiamen Dakai Biotechnology Co ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Transplantation (AREA)
  • Botany (AREA)
  • Cell Biology (AREA)
  • Dermatology (AREA)
  • Medicinal Chemistry (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Zoology (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
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Abstract

一种猪脱细胞板层角膜的制备方法及其猪脱细胞板层角膜，制备方法为：预处理(S1)：对新鲜猪角膜的预处理,制作板层角膜；干燥处理(S2)：将预处理后板层角膜进行干燥处理；采用酶处理方法进行脱细胞(S3)：将干燥后角膜加入酶溶液；置于震荡培养箱中震荡处理；再将角膜置于震荡培养箱震荡洗涤处理，获得脱细胞角膜。灭菌处理：(S4)。

Description

PCT国内申请，说明书已公开。

Claims (1)

  1. PCT国内申请，权利要求书已公开。
CN201680077703.1A 2016-12-16 2016-12-16 一种脱细胞猪角膜的制备方法及其脱细胞板层角膜和用法 Active CN109475663B (zh)

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PCT/CN2016/110458 WO2018107482A1 (zh) 2016-12-16 2016-12-16 一种脱细胞猪角膜的制备方法及其脱细胞板层角膜和用法

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Cited By (1)

* Cited by examiner, † Cited by third party
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CN112168425A (zh) * 2020-04-13 2021-01-05 广东佳悦美视生物科技有限公司 一种人工角膜后板的制备方法

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114259601B (zh) * 2021-12-27 2023-05-26 暨南大学 活细胞仿生角膜前板层及其制备方法
CN115006594B (zh) * 2022-06-26 2023-07-11 中国海洋大学 机械增强型脱细胞猪角膜后板层载体支架制备方法及应用

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CN102294053B (zh) * 2011-07-24 2014-10-29 陕西省眼科研究所 去细胞异种角膜基质载体及其制备方法和应用
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EP1698358A1 (en) * 2003-12-26 2006-09-06 Cardio Incorporated Transplantable biomaterial and method of preparing the same
CN1579342A (zh) * 2004-04-28 2005-02-16 浙江大学医学院附属邵逸夫医院 一种无细胞的异种角膜基质及制备方法和用途
WO2011154687A1 (en) * 2010-06-11 2011-12-15 Ucl Business Plc Biomimetic corneal tissue
CN101947144A (zh) * 2010-09-29 2011-01-19 厦门大学 一种板层组织工程角膜支架及其制作方法与应用
CN101985051A (zh) * 2010-10-21 2011-03-16 暨南大学 一种脱细胞角膜或脱细胞角膜基质及其制备方法和用途
CN103908700A (zh) * 2013-01-06 2014-07-09 陕西佰傲再生医学有限公司 一种脱细胞角膜的制备方法
CN104001214A (zh) * 2014-05-28 2014-08-27 青岛中皓生物工程有限公司 一种板层角膜基质支架及其制备方法和应用
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112168425A (zh) * 2020-04-13 2021-01-05 广东佳悦美视生物科技有限公司 一种人工角膜后板的制备方法

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