CN109463391A - A kind of compound formulation, control method and the application of prevention and control peach branch blight - Google Patents
A kind of compound formulation, control method and the application of prevention and control peach branch blight Download PDFInfo
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- CN109463391A CN109463391A CN201811568232.1A CN201811568232A CN109463391A CN 109463391 A CN109463391 A CN 109463391A CN 201811568232 A CN201811568232 A CN 201811568232A CN 109463391 A CN109463391 A CN 109463391A
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- Prior art keywords
- peach
- prochloraz
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- control
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/60—1,4-Diazines; Hydrogenated 1,4-diazines
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/38—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< where at least one nitrogen atom is part of a heterocyclic ring; Thio analogues thereof
Abstract
The present invention relates to the prevention and treatment of plant disease, belong to disease control research and the application field in plant protection subject.More particularly to compound formulation, control method and the application of a kind of prevention and control peach branch blight, including the trimming of sick branch, clear garden, sick branch residuum is transported into peach garden and concentrates buried, reduction disease infestation bacterium source；Peach leaf fall period and spring peach bud sprouting period application Zhongshengmycin and one of Prochloraz, two kinds of complexing agents of shenqinmycin and Prochloraz in the fall.The present invention proposes should be using combination control method, the bacterium source radix of field wintering is reduced by the trimming of sick branch and clear garden, in conjunction with the Zhongshengmycin of leaf fall period autumn and winter and peach bud germination period and Prochloraz, the biological pesticide of shenqinmycin and Prochloraz and chemical pesticide composite application, it can be effectively controlled peach branch blight and occur and endanger.
Description
Technical field
The present invention relates to the prevention and treatment of plant disease, belong to biological control in plant protection subject and chemical prevention research with
Application field.More particularly to compound formulation, control method and the application of a kind of prevention and control peach branch blight.
Background technique
Peach branch blight also known as contracting canker of hanging are the serious plant diseases that south China peach producing region occurs in recent years, seriously
Influence peach growth and fruit yield.In recent years, south China peach main producing region, such as jiangsu wuxi, Changzhou, Zhejiang Jiaxing, Shanghai
The ground such as Nanhui, Kunming, Yunnan, Sichuan Chengdu, the disease start to occur and constantly spread, and cause serious shadow to peach industry development
It rings.Currently, the disease has become the Major Diseases for endangering peach industry in the multiple peach producing regions in south, fall ill peach average loss 20-
50%, the more serious peach of site morbidity faces the danger of total crop failure.Chemical prevention is the main means for controlling the disease, however fruit
Garden increases pesticide dosage, pollution environment and the destruction ecological balance throughout the year using that germ can be made to develop drug resistance.Biological control is because right
Safety of human and livestock, environment compatibility are good, germ is not likely to produce drug resistance, arouse widespread concern, but its there is also fields
The problems such as drug effect is slow, preventive effect is unstable.And the control strategy that is combined with chemical pesticide of biology can effective disease and pest control and
Chemical pesticide usage amount can be reduced, China's pesticide reduction policy is met.
Biological pesticide:
Zhongshengmycin is a kind of farm antibiotics that biological control research institute, the Chinese Academy of Agricultural Sciences develops, and is become by Strepiomyces lavendulae Hainan
Kind fermentation is made, and belongs to glycoside antibiotic.A kind of protective fungicide that fungicidal spectrum is wider, have tag, osmosis.
Zhongshengmycin has very high activity to bacterial disease and some fungi the venereal disease evil of crops, while the anti-of plant can be improved
Sick ability.Using safe, start to apply in the prevention and treatment of the fruit diseases such as apple, grape.
Shenqinmycin is Life Science and Technology institute, Shanghai Communications University and the happy limited public affairs of biological products share of Shanghai agriculture
The novel microbial source environment friendly agricultural for taking charge of cooperative development is a kind of antibiotic generated by pseudomonas fluorescens M18 fermentation ---
Phenazine-1-carboxylic acid inhibits plant pathogenic fungi with wide spectrum and promotes the dual function of Effects on Plant Growth, has wide spectrum, height
The characteristics of effect, the card of acquisition agriculture chemical registration in 2011 have been used for multiple kinds of crops fungal disease prevention and control, preventive effect is significant.
Chemical pesticide:
Prochloraz is imidazoles fungicide, by inhibiting the biosynthesis of ergosterol, to make somatic cells film function
It is damaged and works, be a kind of high-efficiency low-toxicity fungicide, have and protect and treat double action, be at home and abroad widely used in anti-
The cereal as caused by a variety of disease fungus, oil plant and industrial crops disease are controlled, is the main medicine of current peach branch blight chemical prevention
One of agent.
Since Prochloraz is conventional dose, long-time service easily causes pesticide residue, endangers environment, does not meet the agricultural in China
Green Development strategy is then reduced using imperative.
Summary of the invention
It is used to reduce chemical pesticide, effectively prevents peach branch blight, the present invention provides a kind of answering for prevention and control peach branch blight
Preparation, control method and application are closed, biological pesticide and chemical pesticide directly can be used in mixed way or be developed into dosage form, produce different multiple
Object alternating rotation is closed to use.The usage amount of Prochloraz can not only be reduced by compounding us, reduces environmental pressure, additionally it is possible to mention
Preventive effect of the height to peach branch blight.
In order to achieve the above-mentioned object of the invention, a kind of the technical solution adopted by the present invention are as follows: compound system for preventing and treating peach branch blight
Agent, which is characterized in that compounded by Zhongshengmycin and Prochloraz according to the ratio of 1:1,1:3 or 3:1；Or by shenqinmycin
It is compounded with Prochloraz according to the ratio of 1:1,1:3 or 3:1, the ratio is mass ratio.
A kind of method of prevention and control peach branch blight, specific steps are as follows: sick branch trimming, clear garden are carried out in winter, by sick branch residuum
Peach garden is transported to concentrate buried or burn；Peach leaf fall period and spring peach bud sprouting period application Zhongshengmycin and Prochloraz are answered in the fall
Close one of preparation, shenqinmycin and compound formulation of Prochloraz.
The preparation method of compound formulation are as follows: weigh Zhongshengmycin and Prochloraz or shenqinmycin and Prochloraz, use are water-soluble
It pours into aqueous medical fluid configuration bucket, mixes after solution, install spraying instrument application additional.
Clear garden removes cladoptosis of falling leaves in peach garden；Trimming cuts off disease branch.
The present invention also protects application of the above-mentioned compound formulation in prevention and treatment peach branch blight bacterium.
Biological pesticide Zhongshengmycin and Prochloraz compounding of the present invention has synergistic effect, there is the potentiality of prevention and treatment peach branch blight.
Indoor toxicity test shows that Zhongshengmycin Prochloraz 1:1 and 1:3 compounding have synergistic effect to the withered virulence of peach branch, and 3:1 is multiple
Proportion also has summation action.
Biological pesticide shenqinmycin of the present invention and Prochloraz compounding have synergistic effect, there is the potentiality of prevention and treatment peach branch blight.
Indoor toxicity test shows that shenqinmycin is compounded by 1:1,1:3,3:1 with Prochloraz and has apparent virulence synergist to peach branch blight bacterium
Effect.
Compared with the existing technology, the invention has the benefit that biological pesticide is compounded with chemical pesticide Prochloraz to peach branch
Blight bacterium has virulence synergist effect, can effectively prevent peach branch blight in field, therefore can research and develop novel built agent or direct
It is used in mixed way, reduces chemical pesticide dosage, the green prevention and treatment for peach branch blight.The present invention proposes should be using combination prevention and treatment side
Method reduces the bacterium source radix of field wintering by the trimming of sick branch and clear garden, in conjunction in leaf fall period autumn and winter and peach bud germination period
Raw rhzomorph and Prochloraz, the biological pesticide of shenqinmycin and Prochloraz and chemical pesticide composite application can effectively reach control peach
Branch-rot occurs and harm.
Specific embodiment
Below by way of specific embodiment, invention is further described in detail.
(1) single dose toxicity test
Aseptically, the Zhongshengmycin of various concentration, shenqinmycin, Prochloraz are configured, is grown according to mycelia
Performance rate method, peach branch blight bacterium (Phomopsis amygdali) is on PDA plate after 25 DEG C of culture 5d, with punch on bacterium colony side
Edge beats the bacteria cake that diameter is 6mm, is inoculated into the central (note: after dissolving of culture medium flat plate that different agents are handled respectively with needle is chosen
Culture medium temperature medicament is added when being reduced to 50 DEG C or less, to guarantee the optimum efficiency of medicament), cultivate 5d under the conditions of 25 DEG C
Afterwards, colony diameter is measured with crossing method, each processing is repeated 3 times, and clear water processing is as control.Calculate various concentration medicine
Inhibiting rate [the mycelial growth inhibition rate=(control colony growth diameter-processing bacterium colony growth that agent, bacterium solution grow germ mycelia
Diameter)/control colony growth diameter × 100%].It is several to drug concentration logarithm (abscissa) and corresponding mycelial growth inhibition rate
Rate value (ordinate) carry out regression analysis, by virulence regression equation y=ax+b calculate medicament inhibit mycelia growth it is effective in it is dense
Spend (EC50) it is used as virulence parameter.Toxicity test the result shows that, three kinds of single doses have preferable inhibitory effect to peach branch blight bacterium,
EC50Value is respectively 0.0419 μ g/mL, 0.2314 μ g/mL, 0.0047 μ g/mL (table 1), carries out subsequent compounding co-toxicity and surveys
It is fixed.
(2) compound drug toxicity test
Setting compounded combination: 1) compounding agent is made in tri- ratios of 1:1,1:3 and 3:1 in Zhongshengmycin and Prochloraz raw medicine；
2) compounding agent is made in tri- ratios of 1:1,1:3 and 3:1 in shenqinmycin and Prochloraz raw medicine.
By peach branch blight bacterium bacteria cakeIt is connected to the PDA plate center of the compounding agent containing various concentration, in 25 DEG C of conditions
Colony diameter is measured after lower culture 5d, each processing is repeated 3 times, and the processing without any medicament is as control.Calculate each compounding
The inhibiting rate that agent grows germ mycelia.Ibid regression analysis, which calculates, obtains effective suppression that each proportion mixture grows germ mycelia
Concentration EC in system50Value.The mixed co-toxicity of A and B is evaluated according to following equation to act on, and is calculated SR (synergic ratio):
A, b are ratio of single dose A, B in mixture in formula；EC(A)50、EC(B)50The respectively EC of A, B single dose50Measured value；
EC50(th)For the EC of mixture50Theoretical value；EC50(ob)For the EC of mixture50Measured value.SR indicates the mixed phase interaction of A, B single dose
With: it is antagonism as SR < 0.5；It is summation action when SR=0.5~1.5；It is synergistic effect as SR > 1.5.
The result shows that synergy is presented to the withered virulence of peach branch and makees when Zhongshengmycin compounds proportion 1:1 and 1:3 with Prochloraz
With 3:1 shows summation action when compounding.And three proportions that shenqinmycin is compounded with Prochloraz, it is equal to the withered virulence of peach branch
For synergy (table 1), therefore biological pesticide Zhongshengmycin and shenqinmycin compound with chemical pesticide Prochloraz and can achieve synergy work
With the potentiality with prevention and treatment peach branch blight.
The co-toxicity of 1 Zhongshengmycin of table, shenqinmycin and Prochloraz different ratio
(3) field controling test of compounding agent
2016-2017 continuous 2 years, Zhongshengmycin and shenqinmycin is selected to compound mixture with Prochloraz 1:1, carries out field
Cell peach branch blight controlling experiment, test site are jiangsu wuxi Huishan District Yangshan town, and kind is the white phoenix of wicker.
6 processing are set within 2016 and 2017,45% Prochloraz EW (900 times of preparation diluent), 3% Zhongshengmycin are respectively as follows:
Wettable powder (WP) (800 times of preparation diluent), 1% shenqinmycin suspending agent (SC) (500 times of preparation diluent), Zhongshengmycin with
Prochloraz 1:1 compounding (each concentration halves), shenqinmycin compound (each concentration halves), clear water CK with Prochloraz 1:1.
3 repetitions (cell) of every processing, 3 peach of every cell.Investigate each 25 shoots in every plant of peach east, south, west, north
(100 total), calculates strain disease branch rate, while calculating each processing control efficiency.
2016, the 1st medication of peach bud sprouting period (March 5) carried out the 2nd on March 21, April 1 and April 15 respectively
~4 medications amount to medication 4 times, and 20d and 35d investigates the state of an illness respectively after last time medicine.Test result shows (table 2), after medicine
35d Zhongshengmycin and shenqinmycin single dose are suitable with chemical pesticide Prochloraz preventive effect, 42% or so, and Zhongshengmycin and miaow
The preventive effect of fresh amine equivalent compounding reaches 48.05%, higher than the control efficiency of Zhongshengmycin and Prochloraz single dose, illustrates that compounding plays
Synergy prevention and control effect.
2 Zhongshengmycin of table, shenqinmycin compound the field efficacy to " the white phoenix of wicker " honey peach branch-rot with Prochloraz
(2016)
2017, the 1st medication of peach bud sprouting period (March 9) carried out on March 22, April 13 and April 26 respectively
2~4 medications amount to medication 4 times, and 20d and 35d investigates the state of an illness respectively after last time medicine.Test result shows (table 3), medicine
35d Zhongshengmycin, Zhongshengmycin+Prochloraz preventive effect are preferable afterwards, are all larger than 37%, followed by shenqinmycin+Prochloraz and Shen
Piperazine mycin single dose, preventive effect are above 30%, and compounded combination Zhongshengmycin+Prochloraz and shenqinmycin+Prochloraz preventive effect are high
In Prochloraz single dose, shenqinmycin etc., illustrate that compounding plays the role of synergy prevention and control.
3 Zhongshengmycin of table, shenqinmycin compound the field efficacy to " the white phoenix of wicker " honey peach branch-rot with Prochloraz
(2017)
To sum up, field plot trial in 2 years shows Zhongshengmycin+Prochloraz, shenqinmycin+Prochloraz equivalent compounding
Combination has preferable field branch-rot control efficiency, and preventive effect is equivalent to the preventive effect of single dose Prochloraz, therefore these combinations have
Stablize prevention and treatment peach branch blight effect, can replace in production or rotation uses, reduces chemical pesticide and use effective prevention and control peach simultaneously
Branch-rot achievees the purpose that green prevention and control.
Sick branch residuum is transported peach garden and concentrates buried, reduction disease by preventing control method of the invention, including the trimming of sick branch, clear garden
Evil infects bacterium source；Peach leaf fall period and spring peach bud sprouting period application Zhongshengmycin and Prochloraz, shenqinmycin and miaow are fresh in the fall
One of two kinds of complexing agents of amine.Method is used in combination in biological pesticide and chemical pesticide, respectively weighs according to liquid volume a certain amount of
Medicament dissolved with water after pour into aqueous medical fluid configuration bucket, mix, install spraying instrument application additional.
Preventing control method of the invention is related to bacterium source and removes and the biological pesticide of optimum period and chemical pesticide mixing application.
In the perennial production of fruit trees such as peach, pay attention to the disease control of the season of growth after the fruit tree spring, only often to ensure fruit
Real yield and quality, often but ignores prevention and treatment off season, it is believed that the stage is inessential, with the fruit stage mutually from compared with
Far.The leaf scar scar wound that early-stage study discovery peach of the present invention generates when falling leaves autumn and winter, is that peach branch blight bacterium is mitogenetic
The important channel that spore infects, infect rear germ hide it is overwintering, spring in coming year weather condition be suitable for, directly fall ill, influence
As a result it is extracted out with young sprout.Therefore, it is considered that the prevention and treatment of leaf fall period autumn and winter is extremely important and crucial, and implement controlling experiment.Autumn and winter are set
Prevention and treatment and the clear water that do not prevent and treat compare two comparative test processing, and two kinds of processing spring in coming year Chemical control methods are consistent, in disease
Evil is significant to occur 2 investigation of phase progress, the results showed that autumn and winter prevention and treatment less prevention and treatment can improve preventive effect 26% or so (table 4), explanation
The prevention and treatment of peach branch blight autumn and winter is highly desirable.
Table 4 autumn and winter chemical pesticide control peach branch blight effect
The prevention and treatment of peach branch blight disease cannot only consider chemical prevention, also be integrated in conjunction with a variety of effective means
Prevention and treatment, wherein the cultural control method for reducing bacterium source radix reduction disease generation radix is extremely important.Bacterium source reduces, disease
Incidence and occurrence degree can all mitigate significantly.Investigation early period of the invention is found, containing a large amount of in peach garden disease branch and invalid body
Branch-rot bacterium is the overwintering main place of germ, therefore combines the trimming in peach cultivation management, and sick branch is trimmed and removed
Peach garden invalid body, which should can greatly reduce field, which reduces bacterium source, reduces disease generation radix.The present invention implements the sick branch of trimming and clear
The control method in garden.
Fallen leaves cladoptosis etc. in clear garden, that is, winter removal peach garden；Trimming is that winter beta pruning especially cuts off disease branch.It in addition will be clear
The invalid body of garden and trimming is taken out of buried outside garden or is burnt.Experiments have shown that (table 5), winter clear garden to prevention and mitigates peach with trimming
Branch-rot has remarkable effect, and preventive effect is respectively 37.9% and 44.6%.
Table 5 winter clear garden and trimming act on the prevention and control of peach branch blight
The above display describes basic principles and main features and advantage of the invention.The technical staff of the industry should
Understand, the present invention is not limited to the above embodiments, what is described in the above embodiment and the description is only saying the principle of the present invention,
Without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes and improvements are all
It drops into the claimed scope of the invention.The scope of the present invention is defined by the appended claims and its equivalents.
Claims (6)
1. a kind of compound formulation for preventing and treating peach branch blight, which is characterized in that by Zhongshengmycin and Prochloraz according to 1:1,1:3 or 3:
1 ratio compounds；Or it is compounded by shenqinmycin and Prochloraz according to the ratio of 1:1,1:3 or 3:1, the ratio
Example is mass ratio.
2. a kind of method of prevention and control peach branch blight, which is characterized in that carry out sick branch trimming, clear garden in winter, sick branch residuum is transported
Peach garden is concentrated buried or is burnt out；In the fall the peach leaf fall period and spring peach bud sprouting period application Zhongshengmycin and Prochloraz it is compound
One of preparation, shenqinmycin and compound formulation of Prochloraz.
3. a kind of method of prevention and control peach branch blight according to claim 2, which is characterized in that peach leaf fall period and spring in the fall
Ji Taoya sprouting period applies the compound formulation of prevention and treatment peach branch blight described in claim 1.
4. a kind of method of prevention and control peach branch blight according to claim 2, which is characterized in that the preparation method of compound formulation
Are as follows: Zhongshengmycin and Prochloraz or shenqinmycin and Prochloraz are weighed, aqueous medical fluid configuration bucket is poured into after being dissolved with water
In, it mixes, installs spraying instrument application additional.
5. a kind of method of prevention and control peach branch blight according to claim 2, which is characterized in that clear garden removes peach garden and declines
Leaf cladoptosis；Trimming cuts off disease branch.
6. application of the compound formulation described in claim 1 in prevention and treatment peach branch blight bacterium.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811568232.1A CN109463391A (en) | 2018-12-21 | 2018-12-21 | A kind of compound formulation, control method and the application of prevention and control peach branch blight |
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| CN201811568232.1A CN109463391A (en) | 2018-12-21 | 2018-12-21 | A kind of compound formulation, control method and the application of prevention and control peach branch blight |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103155936A (en) * | 2011-12-18 | 2013-06-19 | 深圳诺普信农化股份有限公司 | Plant disease control composition containing phenazino-1-carboxylic acid |
-
2018
- 2018-12-21 CN CN201811568232.1A patent/CN109463391A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103155936A (en) * | 2011-12-18 | 2013-06-19 | 深圳诺普信农化股份有限公司 | Plant disease control composition containing phenazino-1-carboxylic acid |
Non-Patent Citations (4)
| Title |
|---|
| 尤春梅等: ""生物农药与化学农药复配对桃枝枯病菌的毒力"", 《安徽农业科学》 * |
| 胡寒哲等: ""申嗪霉素和咪鲜胺复配对水稻立枯病菌的增效作用"", 《湖北农业科学》 * |
| 高汝佳: ""桃枝枯病生物防治研究"", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
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