CN109463214B - Method for screening drought-tolerant alfalfa varieties - Google Patents
Method for screening drought-tolerant alfalfa varieties Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/25—Dry fruit hulls or husks, e.g. chaff or coir
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/30—Growth substrates; Culture media; Apparatus or methods therefor based on or containing synthetic organic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
Abstract
The invention discloses a method for screening drought-tolerant alfalfa varieties, which comprises the following steps: sowing the selected alfalfa seeds in a cultivation groove in a drilling mode, and covering the seeds with a substrate after sowing; dividing the obtained alfalfa seedlings into a drought treatment group, a control 1 group and a control 2 group; the drought treatment group adopts organic ecological culture medium for planting, and carries out drought treatment when the alfalfa seedlings grow to 3-4 true leaves, the relative humidity of the medium is reduced to 30% in a stepped mode, and the drought is maintained for a plurality of days; the control group 1 adopts organic ecological culture medium for planting, and the relative humidity of the medium is always maintained at 75%; the control group 2 was planted with soil matrix, and the relative humidity of the matrix was always maintained at 75%; respectively counting the root length of each alfalfa seedling in the drought treatment group, the control group 1 and the control group 2; and calculating the drought resistance value of each alfalfa variety. The screening method has the advantages of low cost, high drought tolerance detection accuracy, short detection period, easy operation and convenient popularization and implementation in various places.
Description
Technical Field
The invention relates to the technical field of plant variety screening, in particular to a method for screening drought-tolerant alfalfa varieties.
Background
Alfalfa, commonly known as burclover, is a perennial flowering plant and has high nutritional value. Alfalfa contains the most abundant vitamin K, has high content of components, and is superior to all vegetables, and other vitamins such as vitamin C, B are also abundant. Alfalfa is a high-quality leguminous forage, has high protein content and good palatability, and is a main source of vegetable protein feed. With the rapid development of animal husbandry in China, the demand for high-quality vegetable protein feed is increasing, and alfalfa with high crude protein content becomes an irreplaceable strategic guarantee forage grass. Drought stress is one of the major factors affecting plant growth and development, causing severe crop loss. Alfalfa belongs to a high water consumption crop, and drought in water resource shortage areas such as northwest of China is one of main factors limiting large-scale and industrialized planting of alfalfa. In recent years, the growth and development of the alfalfa are severely restricted by insufficient water caused by dry climate, reduction of annual precipitation, severe evaporation, increasing desertification, continuous deterioration of ecological environment and other factors, and the alfalfa is an important factor for limiting the popularization and production of the alfalfa in Xinjiang. Many experts and scholars develop researches on the aspects of drought resistance identification, evaluation and the like of alfalfa germplasm resources, and the results show that the adaptability and resistance of different alfalfa varieties to drought are different, and the researches are mainly shown in the aspects of plant morphology, physiology, biomass, root-cap ratio and the like, and the method is still considered to be the most commonly used method for drought resistance identification and evaluation because morphological growth indexes such as plant height, leaf area and the like are sensitive, simple, intuitive, rapid and reliable to water stress; but has the disadvantages of low accuracy and the like. Therefore, the development of the evaluation and screening of the drought-resistant alfalfa varieties with high detection accuracy becomes an imminent task, and the method has extremely important significance for the steady development of alfalfa industrialization in arid and semi-arid regions in China.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides the method for screening the drought-tolerant alfalfa varieties, which has the advantages of low cost, high drought-tolerant detection accuracy, short detection period, easy operation and convenient popularization and implementation in various places.
In order to achieve the purpose, the technical scheme provided by the invention is as follows:
the invention provides a method for screening drought-tolerant alfalfa varieties, which comprises the following steps: step S1, sowing the selected alfalfa seeds in a cultivation groove in a drilling mode, and covering the seeds with a substrate after sowing; thinning when the alfalfa seedlings grow to 1.5-2.5 cm, selecting healthy seedlings with consistent growth vigor, and reserving 50 alfalfa seedlings in each cultivation groove; wherein the substrate is an organic ecological culture substrate or a soil substrate; the relative humidity of the substrate is controlled to be 75 percent; step S2, dividing the alfalfa seedlings into a drought treatment group, a contrast 1 group and a contrast 2 group; the drought treatment group adopts organic ecological culture medium for planting, and carries out drought treatment when the alfalfa seedlings grow to 3-4 true leaves, the relative humidity of the medium is reduced to 30% in a stepped mode, and the drought is maintained for a plurality of days; the control group 1 adopts organic ecological culture medium for planting, and the relative humidity of the medium is always maintained at 75%; the control group 2 was planted with soil matrix, and the relative humidity of the matrix was always maintained at 75%; step S3, respectively counting the root length of each alfalfa seedling in the drought treatment group, the control 1 group and the control 2 group, wherein the average value of the root lengths of 50 alfalfa seedlings in a single cultivation groove is the alfalfa seedling root length of the cultivation groove, and the average value of the alfalfa seedling root lengths of a plurality of cultivation grooves corresponding to each group is the alfalfa seedling root length of the group; step S4, calculating drought resistance values of all alfalfa varieties, wherein the larger the drought resistance value is, the stronger the drought resistance of the corresponding alfalfa variety is; the drought resistance value of a single alfalfa variety is (the relative root length of the drought treatment group of the variety/the average value of the relative root lengths of the drought treatment groups of all the identified varieties) × (the relative root length of the drought treatment group of the variety/the relative root length of the control group 1 of the variety); wherein, the relative root length of the drought treatment group is equal to the root length of the drought treatment group/the root length of the control group 2, and the relative root length of the control group 1 is equal to the root length of the control group 1/the root length of the control group 2. It should be noted that, for the accuracy of drought resistance identification, when the drought resistance value of a single alfalfa variety is calculated, a relative value is adopted so as to eliminate background interference caused by the culture medium.
Preferably, in step S1, the raw material components of the organic ecological culture medium include corn stalks, decomposed cow dung, rice hulls, egg shells, slag and wormcast; the organic ecological culture medium is fermented and decomposed before use.
Further preferably, in step S1, the mass ratio of the corn stalks, the decomposed cow dung, the rice hulls, the egg shells, the slag and the wormcast is 2: 1: 3: 1: 3: 2.
preferably, in step S1, carbendazim is added in an amount of 1g per 10L of the organic ecological culture medium.
Preferably, in step S1, the physicochemical properties of the organic ecological culture medium include: the volume weight is 0.69g/cm3The total porosity was 86%, the gas permeability pore was 22%, the water retention pore was 64%, the pH was 6.8, the conductivity was 2.86ms/cm, and the cation exchange capacity was 65 cmol/kg.
Preferably, the whole screening process is carried out in a smart greenhouse, with a relative humidity of the greenhouse air maintained at 60% and a diurnal temperature of 25/20 ℃.
Preferably, in step S1, the cultivation tank has a length of 1m, a width of 50cm and a depth of 50 cm.
Preferably, in step S1, the covering thickness of the substrate is 0.4-0.6 cm, preferably 0.5 cm.
Preferably, in step S2, the drought-treated group, the control 1 group and the control 2 group respectively include three cultivation tanks.
Preferably, in step S2, the number of days is 18 days.
The technical scheme provided by the invention has the following beneficial effects:
(1) for plants, the development degree of underground root systems determines the strength of drought resistance. Drought resistance of different varieties of the same plant is identified according to the development degree of underground roots, but the development degree of the roots is greatly influenced by the matrix. The culture substrate is more suitable for the growth of plant roots than the soil, for example, the total porosity of the culture substrate is larger than that of the soil substrate. In order to create a good growing environment for the root system, a cultivation substrate suitable for the plant needs to be developed firstly. The invention determines the most suitable formula of the organic ecological alfalfa cultivation medium by researching the influence of the medium with different formulas on the growth vigor, the plant photosynthetic index, the root system, the yield and the quality of the alfalfa.
(2) The method for screening the drought-tolerant alfalfa varieties has the advantages of low cost, high drought-tolerant detection accuracy, short detection period, easiness in operation and convenience in popularization and implementation in various places.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Detailed Description
The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. The following examples are only for illustrating the technical solutions of the present invention more clearly, and therefore are only examples, and the protection scope of the present invention is not limited thereby.
The experimental procedures in the following examples are conventional unless otherwise specified. The test materials used in the following examples were purchased from a conventional store unless otherwise specified. In the quantitative tests in the following examples, three replicates were set, and the data are the mean or the mean ± standard deviation of the three replicates.
Examples
The embodiment provides a method for screening drought-enduring alfalfa varieties, the whole screening process is carried out in an intelligent greenhouse, the relative humidity of greenhouse air is kept at 60%, the day and night temperature is 25/20 ℃, and the screening specifically comprises the following steps:
step S1 (alfalfa seeding): selecting alfalfa seeds, grinding the alfalfa seeds by using sand paper to break hard seeds so as to facilitate germination, then sowing the alfalfa seeds in a cultivation groove with the length of 1m, the width of 50cm and the depth of 50cm, planting the alfalfa seeds in a drilling mode, and keeping a reasonable plant spacing; covering 0.5cm with the substrate after sowing;
thinning when the alfalfa seedlings grow to 2cm, selecting healthy seedlings with consistent growth vigor, and reserving 50 alfalfa seedlings in each cultivation groove on the basis of keeping reasonable plant spacing; wherein the substrate is an organic ecological culture substrate or a soil substrate; the relative humidity of the substrate of all the cultivation tanks is controlled to be 75% from sowing to before drought treatment; the organic ecological culture medium comprises the following raw material components in mass ratio of 2: 1: 3: 1: 3: 2, crushing corn straws, decomposed cow dung, rice hulls, egg shells, furnace slag and wormcast; adding 1g of carbendazim into each 10L of organic ecological culture medium, and fermenting and decomposing the organic ecological culture medium before use; the organic ecological culture medium is not pressed and tamped intentionally when in use, and has the following physical and chemical properties: the volume weight is 0.69g/cm3The total porosity was 86%, the gas permeability pore was 22%, the water retention pore was 64%, the pH was 6.8, the conductivity was 2.86ms/cm, and the cation exchange capacity was 65 cmol/kg.
Step S2 (drought treatment): dividing the alfalfa seedlings into a drought treatment group, a control 1 group and a control 2 group; each of the 3 cultivation tanks is processed, 50 alfalfa seedlings are left in each tank after thinning:
drought treatment group (cultivation substrate drought treatment): planting by adopting an organic ecological culture medium, carrying out drought treatment when the alfalfa seedlings grow to 3-4 true leaves, reducing the relative humidity of the medium to 30% in a stepped manner within a certain time, and maintaining the drought for 18 days;
control 1 group (normal water content of culture medium): planting by adopting an organic ecological culture medium, wherein the relative humidity of the medium is always maintained at 75%;
control 2 group (soil matrix water content normal): the soil matrix is adopted for planting, and the relative humidity of the matrix is always maintained at 75%.
Step S3 (data statistics): respectively counting the root length of each alfalfa seedling in the drought treatment group, the control 1 group and the control 2 group, and calculating the average value for each alfalfa seedling for three times, wherein the average value is the root length of the alfalfa seedling;
the average value of the root length of 50 alfalfa seedlings in a single cultivation groove is the alfalfa seedling root length of the cultivation groove, and the average value of the alfalfa seedling root length of three corresponding cultivation grooves in each group is the alfalfa seedling root length of the group.
Step S4 (drought resistance calculation and drought resistance identification): calculating the drought resistance value of each alfalfa variety, wherein the larger the drought resistance value is, the stronger the drought resistance of the corresponding alfalfa variety is;
the drought resistance value of a single alfalfa variety is (the relative root length of the drought treatment group of the variety/the average value of the relative root lengths of the drought treatment groups of all the identified varieties) × (the relative root length of the drought treatment group of the variety/the relative root length of the control group 1 of the variety);
wherein, the relative root length of the drought treatment group is equal to the root length of the drought treatment group/the root length of the contrast 2 groups;
control 1 group relative root length-control 1 group root length/control 2 group root length.
Comparative example 1
The comparative example provides a method for screening drought-enduring alfalfa varieties, the whole screening process is carried out in an intelligent greenhouse, the relative humidity of the air in the greenhouse is kept at 60%, the day and night temperature is 25/20 ℃, and the screening specifically comprises the following steps:
step S1 (alfalfa seeding): selecting alfalfa seeds, grinding the alfalfa seeds by using sand paper to break hard seeds so as to facilitate germination, then sowing the alfalfa seeds in a cultivation groove with the length of 1m, the width of 50cm and the depth of 50cm, planting the alfalfa seeds in a drilling mode, and keeping a reasonable plant spacing; covering 0.5cm with the substrate after sowing;
thinning when the alfalfa seedlings grow to 2cm, selecting healthy seedlings with consistent growth vigor, and reserving 50 alfalfa seedlings in each cultivation groove on the basis of keeping reasonable plant spacing; wherein the substrate is an organic ecological culture substrate or a soil substrate; the relative humidity of the substrate of all the cultivation tanks is controlled to be 75% from sowing to before drought treatment; the organic ecological culture medium comprises the following raw material components in mass ratio of 2: 1: 3: 3: 2, crushing corn straws, decomposed cow dung, rice hulls, furnace slag and wormcast; adding 1g of carbendazim into each 10L of organic ecological culture medium, and fermenting and decomposing the organic ecological culture medium before use; the organic ecological culture medium is not pressed and tamped intentionally when in use.
Step S2 (drought treatment): dividing the alfalfa seedlings into a drought treatment group, a control 1 group and a control 2 group; each of the 3 cultivation tanks is processed, 50 alfalfa seedlings are left in each tank after thinning:
drought treatment group (cultivation substrate drought treatment): planting by adopting an organic ecological culture medium, carrying out drought treatment when the alfalfa seedlings grow to 3-4 true leaves, reducing the relative humidity of the medium to 30% in a stepped manner within a certain time, and maintaining the drought for 18 days;
control 1 group (normal water content of culture medium): planting by adopting an organic ecological culture medium, wherein the relative humidity of the medium is always maintained at 75%;
control 2 group (soil matrix water content normal): the soil matrix is adopted for planting, and the relative humidity of the matrix is always maintained at 75%.
Step S3 (data statistics): respectively counting the root length of each alfalfa seedling in the drought treatment group, the control 1 group and the control 2 group, and calculating the average value for each alfalfa seedling for three times, wherein the average value is the root length of the alfalfa seedling;
the average value of the root length of 50 alfalfa seedlings in a single cultivation groove is the alfalfa seedling root length of the cultivation groove, and the average value of the alfalfa seedling root length of three corresponding cultivation grooves in each group is the alfalfa seedling root length of the group.
Step S4 (drought resistance calculation and drought resistance identification): calculating the drought resistance value of each alfalfa variety, wherein the larger the drought resistance value is, the stronger the drought resistance of the corresponding alfalfa variety is;
the drought resistance value of a single alfalfa variety is (the relative root length of the drought treatment group of the variety/the average value of the relative root lengths of the drought treatment groups of all the identified varieties) × (the relative root length of the drought treatment group of the variety/the relative root length of the control group 1 of the variety);
wherein, the relative root length of the drought treatment group is equal to the root length of the drought treatment group/the root length of the contrast 2 groups;
control 1 group relative root length-control 1 group root length/control 2 group root length.
Comparative example 2
The comparative example provides a method for screening drought-enduring alfalfa varieties, the whole screening process is carried out in an intelligent greenhouse, the relative humidity of the air in the greenhouse is kept at 60%, the day and night temperature is 25/20 ℃, and the screening specifically comprises the following steps:
step S1 (alfalfa seeding): selecting alfalfa seeds, grinding the alfalfa seeds by using sand paper to break hard seeds so as to facilitate germination, then sowing the alfalfa seeds in a cultivation groove with the length of 1m, the width of 50cm and the depth of 50cm, planting the alfalfa seeds in a drilling mode, and keeping a reasonable plant spacing; covering 0.5cm with the substrate after sowing;
thinning when the alfalfa seedlings grow to 2cm, selecting healthy seedlings with consistent growth vigor, and reserving 50 alfalfa seedlings in each cultivation groove on the basis of keeping reasonable plant spacing; wherein the substrate is an organic ecological culture substrate or a soil substrate; the relative humidity of the substrate of all the cultivation tanks is controlled to be 75% from sowing to before drought treatment; the organic ecological culture medium comprises the following raw material components in mass ratio of 2: 1: 3: 1: 3, crushing corn straws, decomposed cow dung, rice hulls, egg shells and furnace slag; adding 1g of carbendazim into each 10L of organic ecological culture medium, and fermenting and decomposing the organic ecological culture medium before use; the organic ecological culture medium is not pressed and tamped intentionally when in use.
Step S2 (drought treatment): dividing the alfalfa seedlings into a drought treatment group, a control 1 group and a control 2 group; each of the 3 cultivation tanks is processed, 50 alfalfa seedlings are left in each tank after thinning:
drought treatment group (cultivation substrate drought treatment): planting by adopting an organic ecological culture medium, carrying out drought treatment when the alfalfa seedlings grow to 3-4 true leaves, reducing the relative humidity of the medium to 30% in a stepped manner within a certain time, and maintaining the drought for 18 days;
control 1 group (normal water content of culture medium): planting by adopting an organic ecological culture medium, wherein the relative humidity of the medium is always maintained at 75%;
control 2 group (soil matrix water content normal): the soil matrix is adopted for planting, and the relative humidity of the matrix is always maintained at 75%.
Step S3 (data statistics): respectively counting the root length of each alfalfa seedling in the drought treatment group, the control 1 group and the control 2 group, and calculating the average value for each alfalfa seedling for three times, wherein the average value is the root length of the alfalfa seedling;
the average value of the root length of 50 alfalfa seedlings in a single cultivation groove is the alfalfa seedling root length of the cultivation groove, and the average value of the alfalfa seedling root length of three corresponding cultivation grooves in each group is the alfalfa seedling root length of the group.
Step S4 (drought resistance calculation and drought resistance identification): calculating the drought resistance value of each alfalfa variety, wherein the larger the drought resistance value is, the stronger the drought resistance of the corresponding alfalfa variety is;
the drought resistance value of a single alfalfa variety is (the relative root length of the drought treatment group of the variety/the average value of the relative root lengths of the drought treatment groups of all the identified varieties) × (the relative root length of the drought treatment group of the variety/the relative root length of the control group 1 of the variety);
wherein, the relative root length of the drought treatment group is equal to the root length of the drought treatment group/the root length of the contrast 2 groups;
control 1 group relative root length-control 1 group root length/control 2 group root length.
Comparative example 3
The comparative example provides a method for screening drought-enduring alfalfa varieties, the whole screening process is carried out in an intelligent greenhouse, the relative humidity of the air in the greenhouse is kept at 60%, the day and night temperature is 25/20 ℃, and the screening specifically comprises the following steps:
step S1 (alfalfa seeding): selecting alfalfa seeds, grinding the alfalfa seeds by using sand paper to break hard seeds so as to facilitate germination, then sowing the alfalfa seeds in a cultivation groove with the length of 1m, the width of 50cm and the depth of 50cm, planting the alfalfa seeds in a drilling mode, and keeping a reasonable plant spacing; covering 0.5cm with the substrate after sowing;
thinning when the alfalfa seedlings grow to 2cm, selecting healthy seedlings with consistent growth vigor, and reserving 50 alfalfa seedlings in each cultivation groove on the basis of keeping reasonable plant spacing; wherein the substrate is an organic ecological culture substrate or a soil substrate; the relative humidity of the substrate of all the cultivation tanks is controlled to be 75% from sowing to before drought treatment; the organic ecological culture medium comprises the following raw material components in mass ratio of 2: 1: 3: 3: 3: 2, crushing corn straws, decomposed cow dung, rice hulls, egg shells, furnace slag and wormcast; adding 1g of carbendazim into each 10L of organic ecological culture medium, and fermenting and decomposing the organic ecological culture medium before use; the organic ecological culture medium is not pressed and tamped intentionally when in use.
Step S2 (drought treatment): dividing the alfalfa seedlings into a drought treatment group, a control 1 group and a control 2 group; each of the 3 cultivation tanks is processed, 50 alfalfa seedlings are left in each tank after thinning:
drought treatment group (cultivation substrate drought treatment): planting by adopting an organic ecological culture medium, carrying out drought treatment when the alfalfa seedlings grow to 3-4 true leaves, reducing the relative humidity of the medium to 30% in a stepped manner within a certain time, and maintaining the drought for 18 days;
control 1 group (normal water content of culture medium): planting by adopting an organic ecological culture medium, wherein the relative humidity of the medium is always maintained at 75%;
control 2 group (soil matrix water content normal): the soil matrix is adopted for planting, and the relative humidity of the matrix is always maintained at 75%.
Step S3 (data statistics): respectively counting the root length of each alfalfa seedling in the drought treatment group, the control 1 group and the control 2 group, and calculating the average value for each alfalfa seedling for three times, wherein the average value is the root length of the alfalfa seedling;
the average value of the root length of 50 alfalfa seedlings in a single cultivation groove is the alfalfa seedling root length of the cultivation groove, and the average value of the alfalfa seedling root length of three corresponding cultivation grooves in each group is the alfalfa seedling root length of the group.
Step S4 (drought resistance calculation and drought resistance identification): calculating the drought resistance value of each alfalfa variety, wherein the larger the drought resistance value is, the stronger the drought resistance of the corresponding alfalfa variety is;
the drought resistance value of a single alfalfa variety is (the relative root length of the drought treatment group of the variety/the average value of the relative root lengths of the drought treatment groups of all the identified varieties) × (the relative root length of the drought treatment group of the variety/the relative root length of the control group 1 of the variety);
wherein, the relative root length of the drought treatment group is equal to the root length of the drought treatment group/the root length of the contrast 2 groups;
control 1 group relative root length-control 1 group root length/control 2 group root length.
The alfalfa root growth conditions of the inventive examples, the comparative examples 1 to the comparative example 3 were compared, and the specific results are shown in the following table 1.
TABLE 1 growth of alfalfa roots of different groups
It is to be noted that, unless otherwise specified, technical or scientific terms used herein shall have the ordinary meaning as understood by those skilled in the art to which the invention pertains. Unless specifically stated otherwise, the relative steps, numerical expressions, and values of the components and steps set forth in these embodiments do not limit the scope of the present invention. In all examples shown and described herein, unless otherwise specified, any particular value should be construed as merely illustrative, and not restrictive, and thus other examples of example embodiments may have different values.
In the description of the present invention, it is to be understood that the terms "first", "second" and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implying any number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention, and they should be construed as being included in the following claims and description.
Claims (9)
1. A method for screening drought-tolerant alfalfa varieties is characterized by comprising the following steps:
step S1, sowing the selected alfalfa seeds in a cultivation groove in a drilling mode, and covering the seeds with a substrate after sowing; thinning when the alfalfa seedlings grow to 1.5-2.5 cm, selecting healthy seedlings with consistent growth vigor, and reserving 50 alfalfa seedlings in each cultivation groove; wherein the substrate is an organic ecological culture substrate or a soil substrate; the relative humidity of the substrate is controlled to be 75 percent;
step S2, dividing the alfalfa seedlings into a drought treatment group, a contrast 1 group and a contrast 2 group; the drought treatment group adopts organic ecological culture medium for planting, and carries out drought treatment when the alfalfa seedlings grow to 3-4 true leaves, the relative humidity of the medium is reduced to 30% in a stepped mode, and the drought is maintained for a plurality of days; the control group 1 adopts organic ecological culture medium for planting, and the relative humidity of the medium is always maintained at 75%; the control group 2 was planted with soil matrix, and the relative humidity of the matrix was always maintained at 75%;
step S3, respectively counting the root length of each alfalfa seedling in the drought treatment group, the control 1 group and the control 2 group, wherein the average value of the root lengths of 50 alfalfa seedlings in a single cultivation groove is the alfalfa seedling root length of the cultivation groove, and the average value of the alfalfa seedling root lengths of a plurality of cultivation grooves corresponding to each group is the alfalfa seedling root length of the group;
step S4, calculating drought resistance values of all alfalfa varieties, wherein the larger the drought resistance value is, the stronger the drought resistance of the corresponding alfalfa variety is; the drought resistance value of a single alfalfa variety is (the relative root length of the drought treatment group of the variety/the average value of the relative root lengths of the drought treatment groups of all the identified varieties) × (the relative root length of the drought treatment group of the variety/the relative root length of the control group 1 of the variety); wherein, the relative root length of the drought treatment group is equal to the root length of the drought treatment group/the root length of the contrast 2 group, and the relative root length of the contrast 1 group is equal to the root length of the contrast 1 group/the root length of the contrast 2 group;
in the step S1, the raw material components of the organic ecological culture medium include corn stalks, decomposed cow dung, rice hulls, egg shells, slag and wormcast; the organic ecological culture medium is fermented and decomposed before use;
in the step S1, the mass ratio of the corn stalks, the decomposed cow dung, the rice hulls, the egg shells, the slag, and the wormcast is 2: 1: 3: 1: 3: 2.
2. the method of screening a drought tolerant alfalfa variety as claimed in claim 1, characterized in that: in the step S1, 1g of carbendazim is added to every 10L of organic ecological culture medium.
3. The method of screening a drought tolerant alfalfa variety as claimed in claim 1, characterized in that: in step S1, the physicochemical properties of the organic ecological culture medium include: the bulk density was 0.69g/cm3, the total porosity was 86%, the gas permeability was 22%, the water retention pore was 64%, the pH was 6.8, the conductivity was 2.86ms/cm, and the cation exchange capacity was 65 cmol/kg.
4. The method of screening a drought tolerant alfalfa variety as claimed in claim 1, characterized in that: the whole screening process was carried out in an intelligent greenhouse with a relative humidity of 60% in the greenhouse air and a temperature of 25/20 ℃ around the clock.
5. The method of screening a drought tolerant alfalfa variety as claimed in claim 1, characterized in that: in the step S1, the cultivation tank has a length of 1m, a width of 50cm, and a depth of 50 cm.
6. The method of screening a drought tolerant alfalfa variety as claimed in claim 1, characterized in that: in the step S1, the covering thickness of the substrate is 0.4-0.6 cm.
7. The method of screening for drought tolerant alfalfa varieties according to claim 1 or 6, characterized in that: in step S1, the thickness of the substrate is 0.5 cm.
8. The method of screening a drought tolerant alfalfa variety as claimed in claim 1, characterized in that: in step S2, the drought-treated group, the control 1 group, and the control 2 group include three cultivation tanks, respectively.
9. The method of screening a drought tolerant alfalfa variety as claimed in claim 1, characterized in that: in the step S2, the number of days is 18 days.
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| CN103270871A (en) * | 2013-05-16 | 2013-09-04 | 中国农业科学院兰州畜牧与兽药研究所 | Method for fast detecting drought resistance of alfalfa varieties and sorting drought-resistant alfalfa varieties |
| CN105393814A (en) * | 2015-10-13 | 2016-03-16 | 黑龙江八一农垦大学 | Alfalfa drought tolerance identification method |
| CN106717973A (en) * | 2017-01-04 | 2017-05-31 | 甘肃农业大学 | A kind of method of high frequency zone drought resisting alfalfa variety |
| CN108887142A (en) * | 2018-08-27 | 2018-11-27 | 东北农业大学 | A kind of full nutrition organic ecological soilless culture substrate formula suitable for cucumber |
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| CN103270871A (en) * | 2013-05-16 | 2013-09-04 | 中国农业科学院兰州畜牧与兽药研究所 | Method for fast detecting drought resistance of alfalfa varieties and sorting drought-resistant alfalfa varieties |
| CN105393814A (en) * | 2015-10-13 | 2016-03-16 | 黑龙江八一农垦大学 | Alfalfa drought tolerance identification method |
| CN106717973A (en) * | 2017-01-04 | 2017-05-31 | 甘肃农业大学 | A kind of method of high frequency zone drought resisting alfalfa variety |
| CN108887142A (en) * | 2018-08-27 | 2018-11-27 | 东北农业大学 | A kind of full nutrition organic ecological soilless culture substrate formula suitable for cucumber |
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