CN109452172A - A kind of culture of Chinese herbaceous peony seedling and cultural method - Google Patents
A kind of culture of Chinese herbaceous peony seedling and cultural method Download PDFInfo
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- CN109452172A CN109452172A CN201811525873.9A CN201811525873A CN109452172A CN 109452172 A CN109452172 A CN 109452172A CN 201811525873 A CN201811525873 A CN 201811525873A CN 109452172 A CN109452172 A CN 109452172A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C23/00—Distributing devices specially adapted for liquid manure or other fertilising liquid, including ammonia, e.g. transport tanks or sprinkling wagons
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/60—Flowers; Ornamental plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/23—Wood, e.g. wood chips or sawdust
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
Abstract
The invention discloses a kind of culture of Chinese herbaceous peony seedling and cultural methods, take stem segment with axillary bud in the Chinese herbaceous peony of current year life, it is rinsed well with tap water, remove outer scale, base portion injured areas is cut off, impregnates 10-20min with dish washing liquid, then rinse 15-30min with flowing water, then it is finally washed 6-7 times with sterile distilled water on super quiet workbench first with 75% alcohol disinfecting 5-15min；Scalpel after taking out stem section sterilizing is cut into 0.5-1cm；The invention, improve Chinese herbaceous peony seedling cultivated in anti-melting brown rate, avoid some seedling by browning, to improve its survival rate；Nutrition Soil has the effects of absorbing a large amount of moisture, deposit nutrient and ventilation；Rare earth can be made to give full play to the adjustment effect of plant growth substance；Irrigation equipment avoids damage to seedling, and convenient for adjusting height convenient for rotation, so that pouring uniformly, improves seedling percent.
Description
Technical field
The present invention relates to Chinese herbaceous peony technical fields, and in particular to a kind of culture of Chinese herbaceous peony seedling and cultural method.
Background technique
Chinese herbaceous peony, alias take leave of grass, spend middle chancellor, belong to Dilleniales, Paeoniaceae Paeonia Chinese herbaceous peony group perennial herb.Root tuber
By bearing below rootstock, meat is sturdy, is in spindle or long cylindricality, thick 0.6~3.5cm.Chinese herbaceous peony petal is in obovate, floral disc
For tazza shape, 5~June of florescence spends and be generally born at the top or nearly top axil of stem, the grey color of original seed, petal 5~13
Piece.Garden-variety pattern is abundant, has white, powder, red, purple, yellow, green, black and secondary color etc., 10~30 centimetres of Hua Jing, petal is up to upper
Hundred pieces.Fruit is in spindle, and seed is rounded, oblong or point are round.
Conventional method still has the disadvantages that 1, cannot improve Chinese herbaceous peony during Chinese herbaceous peony seedling is cultivated with cultivating
Seedling cultivated in anti-melting brown rate, cause some seedling by browning, to reduce its survival rate；2, Nutrition Soil does not have suction
The effects of receiving a large amount of moisture, deposit nutrient and ventilation；Although 3, existing patent has rare earth to be added in nutrient solution, do not have
There is the form of the concentration and rare earth that can reach required, therefore rare earth cannot be made to give full play to the adjusting of plant growth substance
Effect；4, existing irrigation equipment is not easy to adjust height, is easy to damage seedling, and be not easy to rotate, and causes to pour uneven
It is even, reduce the survival rate of seedling.
Summary of the invention
In order to overcome above-mentioned technical problem, the purpose of the present invention is to provide a kind of cultures of Chinese herbaceous peony seedling and cultivation side
Method, solution must not improve Chinese herbaceous peony seedling cultivated in anti-melting brown rate, cause some seedling by browning, survived to reduce it
Rate；Nutrition Soil does not have the effects of absorbing a large amount of moisture, deposit nutrient and ventilation；Although existing patent has rare earth to be added to
In nutrient solution, but the form of required concentration and rare earth can not be reached, therefore rare earth cannot be made to give full play to plant
The adjustment effect of object growth stimulator；Existing irrigation equipment is not easy to adjust height, is easy to damage seedling, and be not easy to rotate,
Cause to pour unevenly, reduces the technical problems such as the survival rate of seedling.
The purpose of the present invention can be achieved through the following technical solutions:
A kind of culture of Chinese herbaceous peony seedling and cultural method, comprising the following steps:
1) preparation of explant: taking stem segment with axillary bud in the Chinese herbaceous peony of current year life, rinsed well with tap water, removes outer
Layer scale, cuts off base portion injured areas, 10-20min is impregnated with dish washing liquid, then rinse 15-30min with flowing water, then super quiet
0.1HgCl is then first used again with 75% alcohol disinfecting 5-15min on workbench210-20s is sterilized, is finally washed with sterile distilled water
It washs 6-7 times；Scalpel after taking out stem section sterilizing is cut into 0.5-1cm；
2) it pre-processes: stem section being put into PVP solution and impregnates 10-20min, is then taken out；
3) it cultivates: by the stem section in step 2), being inoculated into the culture of 1/2MS+GA31mg/L+6-NB1mg/L+NAA1mg/L
Fiber differentiation on base, and the Eurya plant lemon acid of 0.2g/L is added, uniform cut down of obtained growth is then inoculated into 1/
2MS+6-NB1mg/L+KT0.5mg/L culture medium on Multiplying culture, and the ascorbic acid of 0.2g/L is added, obtains tissue-cultured seedling, most
The tissue-cultured seedling for choosing 2-3cm high afterwards, cuts from base portion, is seeded in dark culture 10 days on the culture medium of 1/2MS+IBA1mg/L, so
It goes to afterwards and the 1/2MS+0.3%AC culture medium of any hormone is not added normally is cultivated；Obtain seedling；
4) preparation of compost: the compost is prepared by following step: by sand, garden ground surface soil, peat soil, water
Moss peat, compost, vermiculite, perlite, soddy soil, yellow soil, burn soil, forest humus soil, bark powder, rice hull ash, without
Cultivation mountain soil and pond sludge are mixed evenly, and then plus water, adjusting moisture content are the 60-64% of all raw material gross weights,
After mixing evenly, after being completely dissolved, matrix is obtained；The formalin and matrix for being diluted to 0.15% using 10-50 parts mix well again
It is covered with air-locked material, closed 48 hours, then opens covering, then through airing in 10-15 days, medicament smell is made to disappear,
And adjusting pH value is 5.6-6.4, obtains compost；
5) it the preparation of nutrient solution: is prepared by the following steps to obtain:
A: 10-15 parts of ferrous nitrates, 10-15 parts of manganese, copper, the nitrate of zinc and 1-3 parts of molybdenums are dissolved in 30-60 parts of water
1-6 parts of Eurya plant lemon acid and 1-6 parts of tartaric acid intermixtures are slowly added under stiring in sour ammonium, in 40-50 DEG C of stirring 20min, system
At manganese iron chelating intermediate；
B: being added 20-30 parts of EDTA in 30-60 parts of water, under stiring, 10-20 parts of calcium nitrate, 10-15 parts of nitric acid is added
Magnesium and 5-10 parts of boric acid, are stirred at room temperature 30-40min, and calcium and magnesium chelating intermediate is made；
C: taking 50-110 parts of nitric acid rare earth saturated solutions, and 30-50 parts of EDTA and 30- are added into saturated solution under strong stirring
60 parts of EDTMP solution adjust pH value to 4-4.5 with potassium hydroxide solution, and reaction temperature is 60-70 DEG C, continuously stir 30min,
It is transparent to solution, rare earth chelating intermediate is made；
D: take the 30-50 parts of EDTA aqueous solutions for containing rare earth oxide 0.3% spare as continuous liquid；Add into reactor
Enter the calcium and magnesium prepared and manganese iron chelating intermediate, stir 30min, be heated to 40 DEG C, continuous liquid is then added, reacts 30min
It is added afterwards and is slowly added to rare earth chelating intermediate, stir 30min, transparent chain chelating intermediate stoste is made, is then added
The aqueous solution that 10-25 parts of urea, 10-15 parts of phosphoric acid, 10-30 parts of potassium nitrate and 5-10 parts of potassium sulfates are mixed into, is stirred
Nutrient solution both can be obtained in 30min；
6) implanting and cultivating: will culture obtains in step 3) seedling, be transplanted in compost and cultivate, by line-spacing 55-60cm,
Spacing in the rows 50-55cm digs planting pit, aperture 15-20cm, and one plant of one cave is colonized, and air humidity control is 65-82%, environment
Temperature is 15-25 DEG C, and by irrigation rig to its regular nutrient solution.
As a further solution of the present invention: dosage of sucrose 15-30g/L, agar consumption in culture medium in the step 3)
10-15g/L, pH value 5.6-5.8, and pass through 110-130 DEG C of autoclave sterilization 15-30min.
As a further solution of the present invention: Fiber differentiation and Multiplying culture are every bottle of inoculation one in the step 3)
Stem section, two bottles of a people, cultivation temperature are 23-27 DEG C, light application time 12h/d, intensity of illumination 1500lx.
As a further solution of the present invention: compost is prepared by following weight parts raw material in the step 4): 10-
20 parts of sands, 5-8 parts of garden ground surface soils, 9-12 parts of peat soils, 4-9 portions of sphagbum peats, 3-6 parts of compost, 10-15 parts of vermiculites, 2-9 parts
Perlite, 7-10 part soddy soil, 1-10 parts of yellow soils, 5-13 parts of baked wheaten cake soil, 5-10 parts of forest humus soil, 6-9 parts of bark powders,
5-9 parts of rice hull ash, 3-8 parts without cultivation mountain soil and 2-10 parts of pond sludge.
As a further solution of the present invention: irrigation rig includes crossbeam, sliding rail, connecting plate, rotation in the step 6)
Motor, first gear, the first spray tube, drainage hole, threeway, the second spray tube, teeth, bearing, water inlet pipe, servo motor, company
The bottom center of extension bar, U-shaped sliding block, second gear, rack gear, transmission shaft, limited block and fixed disk, the crossbeam is fixedly mounted
There is upright slide rail, the side bolt of the sliding rail is fixedly connected with vertical rack gear, U-shaped cunning is slidably connected on the sliding rail
Block, the side of the U-shaped sliding block are fixedly connected with servo motor, transmission are fixedly connected on the output shaft of the servo motor
Axis, the transmission shaft are located at U-shaped sliding block hollow portion, and are sequentially connected with U-shaped sliding block, and the is socketed at the center of the transmission shaft
Connection is engaged with rack in two gears, the second gear, and U-shaped sliding block is driven to move up and down, the bottom of the U-shaped sliding block by pair
Two connecting rods of installation are claimed to connect with connecting plate, the bottom side of the connecting plate is fixedly connected with rotating electric machine, the rotation
First gear is installed, the bottom of the connecting plate is provided with water inlet pipe, and the water inlet pipe protrudes into threeway on the output shaft of motor
Top water inlet in, and by bearing connect, the bottom of the connecting plate is fixedly connected with fixed disk, and the fixed disk position
Outside water inlet pipe, the top of the threeway and fixed disk are slidably connected by ring slider, and the ring slider setting is solid
In the annular groove opened up inside price fixing, the outside of the threeway is provided with teeth, and the water outlet of threeway bottom two seals respectively
It is connected with the first spray tube and the second spray tube, the side of first spray tube and the second spray tube is uniformly provided with draining
Hole.
As a further solution of the present invention: the bottom end of the rack gear is fixedly installed with limited block.
As a further solution of the present invention: the first gear engages connection, driving threeway rotation with teeth.
Beneficial effects of the present invention:
1, pass through pretreatment: stem section being put into PVP solution and impregnates 10-20min, is then taken out, at the same Fiber differentiation,
When Multiplying culture, seedling culture, handled respectively by Eurya plant lemon acid, ascorbic acid and active carbon, to improve Chinese herbaceous peony
The anti-melting brown rate of medicine seedling, avoids it by browning；It is cultivated by Nutrition Soil, nutrient solution is irrigated, and Chinese herbaceous peony can be improved
The survival rate of seedling；
2, sphagbum peat retention ability is strong, and cation replacement ability with higher is conducive to plant absorption nutrition；Vermiculite category
In the inert base that character is stable, there is good venting and water permeable water retention property；Quality is light, easy to carry；High-temperature sterilization is without disease
Insect pest；Cation exchange capacity is very high, can lay in nutrient and gradually discharge, and be in neutrality good cushion performance, can absorb
A large amount of moisture；Perlite increases the aeration of compost, and Nutrition Soil can be prevented hardened；
3, by chain chelating method, calcium and magnesium, manganese iron chelating intermediate and the EDTA of rare earth oxide 0.3% is water-soluble
Liquid, rare earth chelating intermediate and various fertilizer are indissoluble, synthesize one kind and contain a great number of elements and high concentration rare earth simultaneously
Nutrient solution；The light rare earths such as lanthanum, cerium can promote absorption and utilization of the seedling to nitrogen, phosphorus, potassium as growth of seedling regulator,
Increase the chlorophyll of seedling, photosynthesis enhancing is conducive to the accumulation of dry matter, enhances the disease-resistant and antipollution energy of seedling
Power, it is final to improve yield, disease is reduced, survival rate is improved；
4, it is rotated by servo motor, electric transmission axis rotation, transmission shaft drives second gear rotation, second gear and tooth
Item engagement connection, so that U-shaped sliding block is driven to slide up and down on the slide rail, thus drive connecting rod to move up and down by connecting rod,
Both the height of the first spray tube and the second spray tube had been adjusted, sprinkling nutrient solution has been carried out convenient for the seedling to different times, both to rigid
The seedling of cultivation turns down the height of the first spray tube and the second spray tube, has avoided damage to seedling, can to seedling after molding
It the height of the first spray tube and the second spray tube is turned up, is easy to use, improves seedling percent；Water inlet pipe and external hose connect
It connects, threeway is connect with water inlet pipe by bearing, and certain height is arranged in the top water inlet of threeway, and water is avoided to enter three
It is overflowed after logical from top, rotating electric machine drives first gear to rotate by shaft, and first gear is engaged with teeth, to drive three
Logical rotation, the top of threeway and fixed disk be slidably connected by ring slider, and ring slider is arranged in be opened up inside fixed disk
In annular groove, threeway is avoided to fall off, convenient for its rotation, the first spray tube and the second spray tube is then driven to rotate, the first spray
Shower pipe and the rotation sprinkling of the second spray tube so that spraying range is wider, sprinkling it is more uniform, the seedling avoided is not sprayed
To nutrient solution, some sprinklings are excessive, lead to death, improve its survival rate.
Detailed description of the invention
The present invention will be further described below with reference to the drawings.
Fig. 1 is irrigation rig overall structure diagram of the present invention.
Fig. 2 is sliding rail side structure schematic view in irrigation rig of the present invention.
Fig. 3 is sliding rail overlooking structure diagram in irrigation rig of the present invention.
In figure: 1, crossbeam；2, sliding rail；3, connecting plate；4, rotating electric machine；5, first gear；6, the first spray tube；7, it drains
Hole；8, threeway；9, the second spray tube；10, teeth；11, bearing；12, water inlet pipe；13, servo motor；14, connecting rod；15, U-shaped
Sliding block；16, second gear；17, rack gear；18, transmission shaft；19, limited block；20, fixed disk.
Specific embodiment
Below in conjunction with the embodiment of the present invention, technical scheme in the embodiment of the invention is clearly and completely described,
Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based in the present invention
Embodiment, all other embodiment obtained by those of ordinary skill in the art without making creative efforts, all
Belong to the scope of protection of the invention.
Embodiment 1:
A kind of culture of Chinese herbaceous peony seedling and cultural method, comprising the following steps:
1) preparation of explant: taking stem segment with axillary bud in the Chinese herbaceous peony of current year life, rinsed well with tap water, removes outer
Layer scale, cuts off base portion injured areas, 10-20min is impregnated with dish washing liquid, then rinse 15-30min with flowing water, then super quiet
0.1HgCl is then first used again with 75% alcohol disinfecting 5-15min on workbench210-20s is sterilized, is finally washed with sterile distilled water
It washs 6-7 times；Scalpel after taking out stem section sterilizing is cut into 0.5-1cm；
2) it pre-processes: stem section being put into PVP solution and impregnates 10-20min, is then taken out；
3) it cultivates: by the stem section in step 2), being inoculated into the culture of 1/2MS+GA31mg/L+6-NB1mg/L+NAA1mg/L
Fiber differentiation on base, and the Eurya plant lemon acid of 0.2g/L is added, uniform cut down of obtained growth is then inoculated into 1/
2MS+6-NB1mg/L+KT0.5mg/L culture medium on Multiplying culture, and the ascorbic acid of 0.2g/L is added, obtains tissue-cultured seedling, most
The tissue-cultured seedling for choosing 2-3cm high afterwards, cuts from base portion, is seeded in dark culture 10 days on the culture medium of 1/2MS+IBA1mg/L, so
It goes to afterwards and the 1/2MS+0.3%AC culture medium of any hormone is not added normally is cultivated；Obtain seedling；
4) preparation of compost: the compost is prepared by following step: by sand, garden ground surface soil, peat soil, water
Moss peat, compost, vermiculite, perlite, soddy soil, yellow soil, burn soil, forest humus soil, bark powder, rice hull ash, without
Cultivation mountain soil and pond sludge are mixed evenly, and then plus water, adjusting moisture content are the 60-64% of all raw material gross weights,
After mixing evenly, after being completely dissolved, matrix is obtained；The formalin and matrix for being diluted to 0.15% using 10-50 parts mix well again
It is covered with air-locked material, closed 48 hours, then opens covering, then through airing in 10-15 days, medicament smell is made to disappear,
And adjusting pH value is 5.6-6.4, obtains compost；
5) it the preparation of nutrient solution: is prepared by the following steps to obtain:
A: 10-15 parts of ferrous nitrates, 10-15 parts of manganese, copper, the nitrate of zinc and 1-3 parts of molybdenums are dissolved in 30-60 parts of water
1-6 parts of Eurya plant lemon acid and 1-6 parts of tartaric acid intermixtures are slowly added under stiring in sour ammonium, in 40-50 DEG C of stirring 20min, system
At manganese iron chelating intermediate；
B: being added 20-30 parts of EDTA in 30-60 parts of water, under stiring, 10-20 parts of calcium nitrate, 10-15 parts of nitric acid is added
Magnesium and 5-10 parts of boric acid, are stirred at room temperature 30-40min, and calcium and magnesium chelating intermediate is made；
C: taking 50-110 parts of nitric acid rare earth saturated solutions, and 30-50 parts of EDTA and 30- are added into saturated solution under strong stirring
60 parts of EDTMP solution adjust pH value to 4-4.5 with potassium hydroxide solution, and reaction temperature is 60-70 DEG C, continuously stir 30min,
It is transparent to solution, rare earth chelating intermediate is made；
D: take the 30-50 parts of EDTA aqueous solutions for containing rare earth oxide 0.3% spare as continuous liquid；Add into reactor
Enter the calcium and magnesium prepared and manganese iron chelating intermediate, stir 30min, be heated to 40 DEG C, continuous liquid is then added, reacts 30min
It is added afterwards and is slowly added to rare earth chelating intermediate, stir 30min, transparent chain chelating intermediate stoste is made, is then added
The aqueous solution that 10-25 parts of urea, 10-15 parts of phosphoric acid, 10-30 parts of potassium nitrate and 5-10 parts of potassium sulfates are mixed into, is stirred
Nutrient solution both can be obtained in 30min；
6) implanting and cultivating: will culture obtains in step 3) seedling, be transplanted in compost and cultivate, by line-spacing 55-60cm,
Spacing in the rows 50-55cm digs planting pit, aperture 15-20cm, and one plant of one cave is colonized, and air humidity control is 65-82%, environment
Temperature is 15-25 DEG C, and by irrigation rig to its regular nutrient solution.
Wherein: dosage of sucrose 15-30g/L in culture medium in step 3), agar consumption 10-15g/L, pH value 5.6-5.8,
And pass through 110-130 DEG C of autoclave sterilization 15-30min；Fiber differentiation and Multiplying culture are every bottle of inoculation one in step 3)
A stem section, two bottles of a people, cultivation temperature are 23-27 DEG C, light application time 12h/d, intensity of illumination 1500lx；Training in step 4)
It supports soil to be prepared by following weight parts raw material: 10-20 parts of sands, 5-8 parts of garden ground surface soils, 9-12 parts of peat soils, 4-9 portions of fountain moss
Peat, 3-6 part compost, 10-15 parts of vermiculites, 2-9 parts of perlites, 7-10 parts of soddy soils, 1-10 parts of yellow soils, 5-13 portions of baked wheaten cakes
Soil, 5-10 parts of forest humus soil, 6-9 parts of bark powders, 5-9 parts of rice hull ash, 3-8 parts without the cultivation mountain soil and 2-10 parts of pools
Mud.
Implementation column 2
A kind of culture of Chinese herbaceous peony seedling and cultural method, comprising the following steps:
1) preparation of explant: taking stem segment with axillary bud in the Chinese herbaceous peony of current year life, rinsed well with tap water, removes outer
Layer scale, cuts off base portion injured areas, 10-20min is impregnated with dish washing liquid, then rinse 15-30min with flowing water, then super quiet
0.1HgCl is then first used again with 75% alcohol disinfecting 5-15min on workbench210-20s is sterilized, is finally washed with sterile distilled water
It washs 6-7 times；Scalpel after taking out stem section sterilizing is cut into 0.5-1cm；
2) it cultivates: by the stem section in step 1), being inoculated into the culture of 1/2MS+GA31mg/L+6-NB1mg/L+NAA1mg/L
Then uniform cut down of obtained growth is inoculated into 1/2MS+6-NB1mg/L+KT0.5mg/ by Fiber differentiation on base
Multiplying culture on the culture medium of L obtains tissue-cultured seedling, finally chooses the tissue-cultured seedling of 2-3cm high, cuts from base portion, be seeded in 1/2MS
Dark culture 10 days on the culture medium of+IBA1mg/L, then go to be not added any hormone 1/2MS+0.3%AC culture medium carry out just
Often culture；Obtain seedling；
3) preparation of compost: identical as preparation method in embodiment 1；
4) preparation of nutrient solution: identical as preparation method in embodiment 1；
5) implanting and cultivating: will culture obtains in step 2) seedling, be transplanted in compost and cultivate, by line-spacing 55-60cm,
Spacing in the rows 50-55cm digs planting pit, aperture 15-20cm, and one plant of one cave is colonized, and air humidity control is 65-82%, environment
Temperature is 15-25 DEG C, and by irrigation rig to its regular nutrient solution.
Wherein: dosage of sucrose 15-30g/L in culture medium in step 2), agar consumption 10-15g/L, pH value 5.6-5.8,
And pass through 110-130 DEG C of autoclave sterilization 15-30min；Fiber differentiation and Multiplying culture are every bottle of inoculation one in step 2)
A stem section, two bottles of a people, cultivation temperature are 23-27 DEG C, light application time 12h/d, intensity of illumination 1500lx；Training in step 3)
It supports soil to be prepared by following weight parts raw material: 10-20 parts of sands, 5-8 parts of garden ground surface soils, 9-12 parts of peat soils, 4-9 portions of fountain moss
Peat, 3-6 part compost, 10-15 parts of vermiculites, 2-9 parts of perlites, 7-10 parts of soddy soils, 1-10 parts of yellow soils, 5-13 portions of baked wheaten cakes
Soil, 5-10 parts of forest humus soil, 6-9 parts of bark powders, 5-9 parts of rice hull ash, 3-8 parts without the cultivation mountain soil and 2-10 parts of pools
Mud.
Implementation column 3
A kind of culture of Chinese herbaceous peony seedling and cultural method, comprising the following steps:
1) preparation of explant: identical as 1 preparation method of embodiment；
2) it pre-processes: stem section being put into PVP solution and impregnates 10-20min, is then taken out；
3) it cultivates: identical as 1 cultural method of embodiment；
4) preparation of compost: identical as 1 preparation method of embodiment；
5) implanting and cultivating: will culture obtains in step 3) seedling, be transplanted in compost and cultivate, by line-spacing 55-60cm,
Spacing in the rows 50-55cm digs planting pit, aperture 15-20cm, and one plant of one cave is colonized, and air humidity control is 65-82%, environment
Temperature is 15-25 DEG C, and periodically pours fertilizer to it by irrigation rig.
Wherein: dosage of sucrose 15-30g/L in culture medium in step 3), agar consumption 10-15g/L, pH value 5.6-5.8,
And pass through 110-130 DEG C of autoclave sterilization 15-30min；Fiber differentiation and Multiplying culture are every bottle of inoculation one in step 3)
A stem section, two bottles of a people, cultivation temperature are 23-27 DEG C, light application time 12h/d, intensity of illumination 1500lx；Training in step 4)
It supports soil to be prepared by following weight parts raw material: 10-20 parts of sands, 5-8 parts of garden ground surface soils, 9-12 parts of peat soils, 4-9 portions of fountain moss
Peat, 3-6 part compost, 10-15 parts of vermiculites, 2-9 parts of perlites, 7-10 parts of soddy soils, 1-10 parts of yellow soils, 5-13 portions of baked wheaten cakes
Soil, 5-10 parts of forest humus soil, 6-9 parts of bark powders, 5-9 parts of rice hull ash, 3-8 parts without the cultivation mountain soil and 2-10 parts of pools
Mud.
Embodiment 4:
A kind of culture of Chinese herbaceous peony seedling and cultural method, comprising the following steps:
1) preparation of explant: identical as 1 preparation method of embodiment；
2) it pre-processes: stem section being put into PVP solution and impregnates 10-20min, is then taken out；
3) it cultivates: identical as 1 cultural method of embodiment；
4) preparation of nutrient solution: identical as 1 preparation method of embodiment；
5) implanting and cultivating: will culture obtains in step 3) seedling, be transplanted in compost and cultivate, by line-spacing 55-60cm,
Spacing in the rows 50-55cm digs planting pit, aperture 15-20cm, and one plant of one cave is colonized, and air humidity control is 65-82%, environment
Temperature is 15-25 DEG C, and by irrigation rig to its regular nutrient solution.
Wherein: dosage of sucrose 15-30g/L in culture medium in step 3), agar consumption 10-15g/L, pH value 5.6-5.8,
And pass through 110-130 DEG C of autoclave sterilization 15-30min；Fiber differentiation and Multiplying culture are every bottle of inoculation one in step 3)
A stem section, two bottles of a people, cultivation temperature are 23-27 DEG C, light application time 12h/d, intensity of illumination 1500lx.
A kind of culture of Chinese herbaceous peony seedling and cultural method, comprising the following steps:
2) preparation of explant: identical as 1 preparation method of embodiment；
2) it pre-processes: stem section being put into PVP solution and impregnates 10-20min, is then taken out；
3) it cultivates: identical as 1 cultural method of embodiment；
4) implanting and cultivating: will culture obtains in step 3) seedling, be transplanted in compost and cultivate, by line-spacing 55-60cm,
Spacing in the rows 50-55cm digs planting pit, aperture 15-20cm, and one plant of one cave is colonized, and air humidity control is 65-82%, environment
Temperature is 15-25 DEG C, and periodically pours fertilizer to it by irrigation rig.
Wherein: dosage of sucrose 15-30g/L in culture medium in step 2), agar consumption 10-15g/L, pH value 5.6-5.8,
And pass through 110-130 DEG C of autoclave sterilization 15-30min；Fiber differentiation and Multiplying culture are every bottle of inoculation one in step 3)
A stem section, two bottles of a people, cultivation temperature are 23-27 DEG C, light application time 12h/d, intensity of illumination 1500lx.
Embodiment 1-5 is tested for the property, test result is as shown in table 1:
Table 1
| Implement 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | |
| Anti- melting brown rate/% | 99.9% | 64.2% | 99.8% | 99.9% | 99.8% |
| Survival rate/% | 82.7% | 82.5% | 64.3% | 62.1% | 35.6% |
As seen from the above table, there is good anti-melting brown rate in embodiment 1,3,4 and 5, highest can achieve 99.9%, be because
To be pre-processed: stem section being put into PVP solution and impregnates 10-20min, is then taken out, while in Fiber differentiation, proliferation training
When feeding, seedling is cultivated, handled respectively by Eurya plant lemon acid, ascorbic acid and active carbon, to improve Chinese herbaceous peony seedling
Anti- melting brown rate；
Survival rate implements 3 and 4 and takes second place than highest in Examples 1 and 2, is because passing through nutrition in Examples 1 and 2
Soil is cultivated, and nutrient solution is irrigated, and implements only to joined one of which in 3 and 4, so that survival rate is taken second place, and is implemented
Cultivated in example 5 not over Nutrition Soil, nutrient solution is irrigated, and survival rate can only achieve 35.6%, therefore, Nutrition Soil and
Good water absorption and nutrient are more in nutrient solution, and the survival rate of Chinese herbaceous peony seedling can be improved.
It please refers to shown in Fig. 1-3, irrigation rig includes crossbeam 1, sliding rail 2, connecting plate 3, rotating electric machine 4, first in step 6
Gear 5, the first spray tube 6, drainage hole 7, threeway 8, the second spray tube 9, teeth 10, bearing 11, water inlet pipe 12, servo motor
13, connecting rod 14, U-shaped sliding block 15, second gear 16, rack gear 17, transmission shaft 18, limited block 19 and fixed disk 20, the bottom of crossbeam 1
Upright slide rail 2 is fixedly installed at portion center, the side bolt of sliding rail 2 is fixedly connected with vertical rack gear 17, the upper sliding of sliding rail 2
It is connected with U-shaped sliding block 15, the side of U-shaped sliding block 15 is fixedly connected with servo motor 13, fixes on the output shaft of servo motor 13
It is connected with transmission shaft 18, transmission shaft 18 is located at U-shaped 15 hollow portion of sliding block, and is sequentially connected with U-shaped sliding block 15, in transmission shaft 18
Second gear 16 is socketed at the heart, second gear 16 engages connection with rack gear 17, drives U-shaped sliding block 15 to move up and down, U-shaped sliding block
15 bottom passes through two connecting rods 14 being symmetrically installed and connect with connecting plate 3, and the bottom side of connecting plate 3 is fixedly connected with rotation
Motor 4 is equipped with first gear 5 on the output shaft of rotating electric machine 4, and the bottom of connecting plate 3 is provided with water inlet pipe 12, water inlet pipe 12
It protrudes into the top water inlet of threeway 8, and is connected by bearing 11, the bottom of connecting plate 3 is fixedly connected with fixed disk 20, and solid
Price fixing 20 is located at outside water inlet pipe 12, and top and the fixed disk 20 of threeway 8 are slidably connected by ring slider, ring slider setting
In the annular groove opened up inside fixed disk 20, the outside of threeway 8 is provided with teeth 10, and the water outlet of 8 bottom two of threeway is close respectively
Envelope is connected with the first spray tube 6 and the second spray tube 9, the side row of being uniformly provided with of the first spray tube 6 and the second spray tube 9
Water hole 7；The bottom end of rack gear 17 is fixedly installed with limited block 19, avoids U-shaped sliding block 15 from falling off, causes safety accident；First gear 5
Connection is engaged with teeth 10, driving threeway 8 rotates, convenient for driving the first spray tube 6 and the rotation of the second spray tube 9.
The working principle of the invention: being rotated by servo motor 13, and electric transmission axis 18 rotates, and transmission shaft 18 drives second
Gear 16 rotates, and second gear 16 engages connection with rack gear 17, so that U-shaped sliding block 15 is driven to slide up and down on sliding rail 2, thus
By connecting rod 14 drive connecting rod 14 move up and down, both adjusted the height of the first spray tube 6 and the second spray tube 9, convenient for pair
The seedling of different times carries out sprinkling nutrient solution, both to the seedling just cultivated, turns down the first spray tube 6 and the second spray tube 9
Highly, seedling has been avoided damage to, to seedling after molding, the height of the first spray tube 6 and the second spray tube 9 can be turned up, side
Just it uses, improves seedling percent；Water inlet pipe 12 is connect with external hose, and threeway 8 is connect with water inlet pipe 12 by bearing 11, and
And certain height is arranged in the top water inlet of threeway 8, overflows after avoiding water from entering threeway 8 from top, rotating electric machine 4 passes through
Shaft drives first gear 5 to rotate, and first gear 5 engage with teeth 10, so that threeway 8 be driven to rotate, the top of threeway 8 with admittedly
Price fixing 20 is slidably connected by ring slider, and ring slider is arranged in the annular groove opened up inside fixed disk 20, avoids three
Logical 8 fall off, and convenient for its rotation, the first spray tube 6 and the second spray tube 9 are then driven to rotate, the first spray tube 6 and the second spray
The rotation sprinkling of pipe 9 so that spraying range is wider, sprinkling it is more uniform, the seedling avoided is not sprayed onto nutrient solution, has
Sprinkling excessively, leads to death, improves its survival rate.
In the description of this specification, the description of reference term " one embodiment ", " example ", " specific example " etc. means
Particular features, structures, materials, or characteristics described in conjunction with this embodiment or example are contained at least one implementation of the invention
In example or example.In the present specification, schematic expression of the above terms may not refer to the same embodiment or example.
Moreover, particular features, structures, materials, or characteristics described can be in any one or more of the embodiments or examples to close
Suitable mode combines.
Above content is only citing made for the present invention and explanation, affiliated those skilled in the art are to being retouched
The specific embodiment stated does various modifications or additions or is substituted in a similar manner, and without departing from invention or surpasses
More range defined in the claims, is within the scope of protection of the invention.
Claims (7)
1. a kind of Chinese herbaceous peony seedling culture and cultural method, which comprises the following steps:
1) preparation of explant: stem segment with axillary bud in the Chinese herbaceous peony of current year life is taken, is rinsed well with tap water, removes outer layer squama
Piece cuts off base portion injured areas, 10-20min is impregnated with dish washing liquid, then rinse 15-30min with flowing water, then in super quiet work
0.1HgCl is then first used again with 75% alcohol disinfecting 5-15min on platform210-20s is sterilized, finally washs 6- with sterile distilled water
7 times；Scalpel after taking out stem section sterilizing is cut into 0.5-1cm；
2) it pre-processes: stem section being put into PVP solution and impregnates 10-20min, is then taken out；
3) it cultivates: the stem section in step 2) is inoculated on the culture medium of 1/2MS+GA31mg/L+6-NB1mg/L+NAA1mg/L
Fiber differentiation, and the Eurya plant lemon acid of 0.2g/L is added, uniform cut down of obtained growth is then inoculated into 1/2MS+
Multiplying culture on the culture medium of 6-NB1mg/L+KT0.5mg/L, and the ascorbic acid of 0.2g/L is added, tissue-cultured seedling is obtained, is finally selected
The tissue-cultured seedling for taking 2-3cm high, cuts from base portion, is seeded in dark culture 10 days on the culture medium of 1/2MS+IBA1mg/L, then turns
It is normally cultivated to the 1/2MS+0.3%AC culture medium that any hormone is not added；Obtain seedling；
4) preparation of compost: the compost is prepared by following step: by sand, garden ground surface soil, peat soil, fountain moss mud
Charcoal, vermiculite, perlite, soddy soil, yellow soil, burns soil, forest humus soil, bark powder, rice hull ash, without cultivation at compost
Mountain soil and pond sludge are mixed evenly, and then plus water, adjusting moisture content are the 60-64% of all raw material gross weights, stirring
After uniformly, after being completely dissolved, matrix is obtained；The formalin and matrix for being diluted to 0.15% using 10-50 parts mix well again with not
Ventilative material covering closed 48 hours, then opens covering, then through airing in 10-15 days, medicament smell is made to disappear, and adjust
Section pH value is 5.6-6.4, obtains compost；
5) it the preparation of nutrient solution: is prepared by the following steps to obtain:
A: dissolving 10-15 parts of ferrous nitrates, 10-15 parts of manganese, copper, the nitrate of zinc and 1-3 parts of ammonium molybdates in 30-60 parts of water,
Under stiring, 1-6 parts of Eurya plant lemon acid and 1-6 parts of tartaric acid intermixtures are slowly added and manganese iron is made in 40-50 DEG C of stirring 20min
Chelating intermediate；
B: in 30-60 parts of water be added 20-30 part EDTA, under stiring, addition 10-20 parts of calcium nitrate, 10-15 parts of magnesium nitrates and
5-10 parts of boric acid, are stirred at room temperature 30-40min, and calcium and magnesium chelating intermediate is made；
C: taking 50-110 parts of nitric acid rare earth saturated solutions, is added 30-50 parts EDTA and 30-60 parts into saturated solution under strong stirring
EDTMP solution adjusts pH value to 4-4.5 with potassium hydroxide solution, and reaction temperature is 60-70 DEG C, 30min is continuously stirred, to molten
Liquid is transparent, and rare earth chelating intermediate is made；
D: take the 30-50 parts of EDTA aqueous solutions for containing rare earth oxide 0.3% spare as continuous liquid；It is added into reactor
The calcium and magnesium and manganese iron chelating intermediate of preparation stir 30min, are heated to 40 DEG C, and continuous liquid is then added, and add after reacting 30min
Enter to be slowly added to rare earth chelating intermediate, stirs 30min, transparent chain chelating intermediate stoste is made, 10-25 is then added
The aqueous solution that part urea, 10-15 parts of phosphoric acid, 10-30 parts of potassium nitrate and 5-10 parts of potassium sulfates are mixed into, is stirred 30min, both
Nutrient solution can be obtained；
6) it implanting and cultivating: by the seedling that culture obtains in step 3), is transplanted in compost and cultivates, by line-spacing 55-60cm, spacing in the rows
50-55cm digs planting pit, aperture 15-20cm, and one plant of one cave is colonized, and air humidity control is 65-82%, environment temperature
It is 15-25 DEG C, and by irrigation rig to its regular nutrient solution.
2. a kind of Chinese herbaceous peony seedling culture according to claim 1 and cultural method, which is characterized in that training in the step 3)
Dosage of sucrose 15-30g/L, agar consumption 10-15g/L, pH value 5.6-5.8 in base are supported, and passes through 110-130 DEG C of high temperature and pressure
Sterilize 15-30min.
3. a kind of Chinese herbaceous peony seedling culture according to claim 1 and cultural method, which is characterized in that lured in the step 3)
It leads culture and Multiplying culture is one stem section of every bottle of inoculation, two bottles of a people, cultivation temperature is 23-27 DEG C, and light application time is
12h/d, intensity of illumination 1500lx.
4. a kind of Chinese herbaceous peony seedling culture according to claim 1 and cultural method, which is characterized in that training in the step 4)
It supports soil to be prepared by following weight parts raw material: 10-20 parts of sands, 5-8 parts of garden ground surface soils, 9-12 parts of peat soils, 4-9 portions of fountain moss
Peat, 3-6 part compost, 10-15 parts of vermiculites, 2-9 parts of perlites, 7-10 parts of soddy soils, 1-10 parts of yellow soils, 5-13 portions of baked wheaten cakes
Soil, 5-10 parts of forest humus soil, 6-9 parts of bark powders, 5-9 parts of rice hull ash, 3-8 parts without the cultivation mountain soil and 2-10 parts of pools
Mud.
5. a kind of Chinese herbaceous peony seedling culture according to claim 1 and cultural method, which is characterized in that filled in the step 6)
Irrigating device includes crossbeam (1), sliding rail (2), connecting plate (3), rotating electric machine (4), first gear (5), the first spray tube (6), row
Water hole (7), threeway (8), the second spray tube (9), teeth (10), bearing (11), water inlet pipe (12), servo motor (13), connection
Bar (14), U-shaped sliding block (15), second gear (16), rack gear (17), transmission shaft (18), limited block (19) and fixed disk (20), institute
The bottom center for stating crossbeam (1) is fixedly installed with upright slide rail (2), and the side bolt of the sliding rail (2) is fixedly connected with perpendicular
Spur rack (17) slidably connects U-shaped sliding block (15) on the sliding rail (2), and the side of the U-shaped sliding block (15) is fixedly connected
Have servo motor (13), is fixedly connected with transmission shaft (18) on the output shaft of the servo motor (13), the transmission shaft (18)
It is sequentially connected positioned at U-shaped sliding block (15) hollow portion, and with U-shaped sliding block (15), the is socketed at the center of the transmission shaft (18)
Two gears (16), the second gear (16) engages connection with rack gear (17), U-shaped sliding block (15) is driven to move up and down, described U-shaped
The bottom of sliding block (15) passes through two connecting rods (14) being symmetrically installed and connect with connecting plate (3), the bottom one of the connecting plate (3)
Side is fixedly connected with rotating electric machine (4), is equipped with first gear (5), the connecting plate on the output shaft of the rotating electric machine (4)
(3) bottom is provided with water inlet pipe (12), and the water inlet pipe (12) is protruded into the top water inlet of threeway (8), and passes through bearing
(11) it connects, the bottom of the connecting plate (3) is fixedly connected with fixed disk (20), and the fixed disk (20) is located at water inlet pipe
(12) external, the top of the threeway (8) is slidably connected with fixed disk (20) by ring slider, and the ring slider setting exists
In the annular groove opened up inside fixed disk (20), the outside of the threeway (8) is provided with teeth (10), threeway (8) bottom
The water outlet of portion two is sealedly connected with the first spray tube (6) and the second spray tube (9), first spray tube (6) and the second spray respectively
The side of shower pipe (9) is uniformly provided with drainage hole (7).
6. a kind of Chinese herbaceous peony seedling culture according to claim 5 and cultural method, which is characterized in that the rack gear (17)
Bottom end is fixedly installed with limited block (19).
7. a kind of Chinese herbaceous peony seedling culture according to claim 5 and cultural method, which is characterized in that the first gear
(5) connection, driving threeway (8) rotation are engaged with teeth (10).
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