CN109443870A - Cell centrifugal rotor - Google Patents
Cell centrifugal rotor Download PDFInfo
- Publication number
- CN109443870A CN109443870A CN201811288703.3A CN201811288703A CN109443870A CN 109443870 A CN109443870 A CN 109443870A CN 201811288703 A CN201811288703 A CN 201811288703A CN 109443870 A CN109443870 A CN 109443870A
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- Prior art keywords
- centrifugal
- slide
- cell
- specimen holder
- sample
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
- G01N2001/2846—Cytocentrifuge method
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Centrifugal Separators (AREA)
Abstract
The present invention relates to the field of medical instrument technology, more particularly to a kind of cell centrifugal rotor, including specimen holder and centrifugal mechanism, specimen holder includes the sample cell for placing sample liquid, sample cell is fixedly connected with guide pipe, specimen holder further includes the slide fixed mechanism for clamping slide, and guide pipe is for being connected to sample cell and slide chamber；Centrifugal mechanism includes the rotor block and several centrifugal seats for being used to install specimen holder for connecting with centrifuge, it is offered in centrifugal seat and the matched special-shaped groove of the bottom shape of specimen holder, rotor block is equipped with bottom plate, the edge of bottom plate and the bottom of centrifugal seat are hinged, and rotor block and centrifugal seat are equipped between the side wall of sample cell makes sample cell to the inclined elastic component of rotor block.It can prevent the sample drop in guide pipe from falling in slide fixed mechanism using this programme, can not scratch when taking out slide to the cell of surface of glass slide.
Description
Technical field
The present invention relates to the field of medical instrument technology, and in particular to a kind of cell centrifugal rotor.
Background technique
During the film-making of cell or culture, sample liquid need to be usually centrifuged, by sample by way of centrifugation
Various constituent separation in product liquid, to obtain the research ingredient of needs, or keep the cell of dispersion poly- by way of centrifugation
Collection to help the screening to cell, therefore is highly important to the centrifugation of cell.
Existing cytospin includes cytospin main body and cell centrifugal rotor, cytospin main body provide from
The power of the heart, cell centrifugal rotor are mounted in cytospin main body, are provided on cell centrifugal rotor for placing sample
The sample cavity of liquid can place the sample liquid of various demands by replacing cell centrifugal rotor in cytospin main body.
Existing cell centrifugal rotor includes the sample cavity and slide fixed mechanism for placing print liquid, in slide fixed mechanism
Glass slide be connected to sample cavity by pipeline, slide fixed mechanism picks and places glass slide by way of being inserted into, extracting, and picks and places not
It is convenient, it is easy to scratch when taking out glass slide to smear position, so that the cell of smear position is scraped, influences to examine knot
Fruit.
Secondly, still have sample liquid in sample cavity and pipeline when taking out glass slide, and since glass slide takes out, pipeline
Interior sample liquid may flow in slide fixed mechanism, to drop onto test atmosphere, if the sample liquid examined is to exist
Communicable substance can then pollute test atmosphere, and there are pathophorous risks.
Summary of the invention
Technical problem solved by the invention is to provide a kind of cell centrifugal rotor, can prevent the sample in guide pipe
Drop is fallen in slide fixed mechanism, can not be scratched when taking out slide to the cell of surface of glass slide.
Base case provided by the invention: cell centrifugal rotor, including specimen holder and centrifugal mechanism, specimen holder include being used for
The sample cell of sample liquid is placed, sample cell is fixedly connected with guide pipe, and specimen holder further includes fixing for clamping the slide of slide
Mechanism, slide fixed mechanism include the hinged front cover and rear cover in bottom, and the connector that can be disengaged is equipped between front cover and rear cover,
There is the slide chamber for placing slide between front cover and rear cover, guide pipe is equipped with mounting base, mounting base far from one end of sample cell
It is detachably connected with front cover, guide pipe is for being connected to sample cell and slide chamber；Centrifugal mechanism includes for connecting with centrifuge
Rotor block and several for installing the centrifugal seat of specimen holder, offers and the matched abnormal shape of the bottom shape of specimen holder in centrifugal seat
Slot, rotor block are equipped with bottom plate, and the edge of bottom plate and the bottom of centrifugal seat are hinged, and rotor block and centrifugal seat are close to the side of sample cell
Being equipped between wall makes sample cell to the inclined elastic component of rotor block.
The principle of the present invention and advantage are:
1. rotor block and centrifugal seat are between the side wall of sample cell since the edge of bottom plate and the bottom of centrifugal seat are hinged
Being equipped with makes sample cell to the inclined elastic component of rotor block, and under static state, the distance of centrifugal seat bottom to rotor block is certain,
By the pulling force of elastic component, so that tilted at the top of centrifugal seat to rotor block, since specimen holder is mounted in centrifugal seat, centrifugal seat inclination
Lead to specimen holder also run-off the straight, so that guide pipe is higher than the oral area close to sample cavity close to the oral area of slide chamber, in guide pipe
Sample liquid flow back in sample cavity, to prevent the sample drop in guide pipe from falling in the slide chamber of slide fixed mechanism.
2. the bottom of front cover and rear cover is hinged, connector due to being equipped with the connector that can disengage between front cover and rear cover
After disengagement, by moving front cover and rear cover, slide chamber is opened, by slide along rear cover taking-up, during taking-up, the smear position of slide
Setting will not be in contact with front cover, when taking out slide, not scratch to the cell of surface of glass slide.
It further, further include stator and rotor for generating magnetic field, centrifugal seat is equipped with leading for cutting magnetic induction line
Body is additionally provided with comparator and controller in centrifugal seat, and conductor and comparator form closed circuit, and comparator is electrically connected with control
Device；Connector includes the permanent magnet covered after being set to and the electromagnet on front cover, and controller is for controlling leading for electromagnet
The current direction of electric winding, special-shaped groove include slide portion, and the side wall in slide portion is expanded by rotor block outwardly direction.
Magnetic field is formed in centrifuge by stator and rotor, since centrifugal seat is equipped with leading for cutting magnetic induction line
Body, in centrifugal process, centrifugal seat is rotated by axis of rotor block, the magnetic induction line of conductor cutting magnetic field, generates induced current, induction
Electric current is directly proportional to the speed that centrifugal seat rotates, and when centrifuge starts, revolving speed is gradually risen, and until reaching stabilized (steady-state) speed, and protects
It is fixed to keep steady, and when centrifuge stops, revolving speed is gradually decreased, and until stopping, setting electric current threshold value compares induced current by comparator
Whether current threshold is reached, when induced current reaches current threshold for the first time, centrifuge treating is in starting state, controller control
Conductive winding is powered, and electromagnet attracts permanent magnet, so that front cover and rear cover is further clamped slide, when induced current reaches for the second time
When current threshold, centrifuge will stop, and controller changes the current direction of conductive winding, and electromagnet repels permanent magnet, so that
Rear cover automatically opens slide chamber, can be taken out conveniently slide, and will not scratch to the cell of surface of glass slide.
Further, it is equipped with the protrusion and groove of grafting between the bottom of slide fixed mechanism and slide portion, is equipped in protrusion
Contact, groove is interior to be equipped with the sheet metal that can be contacted with contact.
Due to being equipped with the protrusion and groove of grafting between the bottom and slide portion of slide fixed mechanism, specimen holder is mounted on
When centrifugal seat, the position of specimen holder is capable of fixing by protrusion and groove, secondly, passing through the gold in the contact and groove in protrusion
Category piece is in contact and the conductive winding of electromagnet can be connected.
It further, further include the strip groove on centrifuge inner wall, strip groove is connected with the first L-type by spring slide
Block, centrifugal seat are equipped with the second L-type block that can be disengaged with the first L-type block close to the side wall of centrifuge inner wall.
Since the edge of bottom plate and the bottom of centrifugal seat are hinged, rotor block and centrifugal seat are equal between the side wall of sample cell
Equipped with sample cell is made, to the inclined elastic component of rotor block, by the effect of elastic component, centrifugal seat meeting run-off the straight passes through the first L-type
The cooperation of block and the second L-type block, so that centrifugal seat keeps vertical state, convenient for specimen holder to be put into centrifugal seat；In centrifugal seat
When rotating, the first L-type block and the second L-type block are disengaged, since the first L-type block is connected in strip groove by spring slide,
By the effect of spring, the first L-type block is drawn into strip groove, and the second L-type block is avoided to occur during rotation with the first L-type block
Collision.
Further, sealing ring is equipped between mounting base and front cover.
By sealing ring mounting base is further sealed with front cover, prevents seam of the sample liquid between mounting base and front cover
Gap drippage.
Further, the annular filter paper for absorbing excessive moisture is equipped in sealing ring.
Sample liquid can isolate moisture by centrifugation, and moisture flows to slide chamber as cell passes through guide pipe together, thus
Smear is carried out, when excess moisture will lead to smear, the hypocellularity assembled on slide is unfavorable for observing, due to setting in sealing ring
There is the annular filter paper for absorbing excessive moisture, for sample liquid when flowing through annular filter paper, the moisture in sample liquid is annular
Filter paper absorbs, so that the cell concentration assembled on slide increases, facilitates look at cell.
Further, special-shaped groove further includes guiding pipe portion, and guiding pipe portion offers the arc groove for picking and placing specimen holder.
Due to guiding pipe portion offer arc groove, after the completed centrifugation, by arc groove can facilitate by specimen holder from from
It is taken out in heart seat.
Detailed description of the invention
Fig. 1 is the partial sectional view of cell centrifugal rotor embodiment sample seat of the present invention.
Fig. 2 is the cross-sectional view of cell centrifugal rotor embodiment centrifugal seat of the present invention.
Fig. 3 is the top view of cell centrifugal rotor embodiment centrifugal mechanism of the present invention.
Fig. 4 is the portion the A enlarged diagram of cell centrifugal rotor implementation example figure 1 of the present invention.
Specific embodiment
It is further described below by specific embodiment:
Appended drawing reference in Figure of description include: sample cell 1, guide pipe 2, mounting base 201, front cover 301, rear cover 302,
Slide chamber 303, sealing ring 304, annular filter paper 305, rotor block 4, bottom plate 5, centrifugal seat 6, slide portion 601, guiding pipe portion
602, arc groove 603, centrifuge 7, strip groove 701, the first L-type block 702, the second L-type block 703, permanent magnet 801, electromagnet
802, raised 901, groove 902.
Embodiment
Cell centrifugal rotor, including specimen holder and centrifugal mechanism, as shown in Fig. 1, specimen holder includes for placing sample
The sample cell 1 of liquid, the mouth thread of sample cell 1 are connected with sealing cover, and the bottom of sample cell 1 is arc, on the side wall of sample cell 1
First through hole is offered, first through hole is bonded with guide pipe 2, and guide pipe 2 is equipped with mounting base 201 far from one end of first through hole；
Specimen holder further includes the slide fixed mechanism for clamping slide, and slide fixed mechanism includes the bottom front cover hinged by hinge
301 and rear cover 302, there is slide chamber 303 between front cover 301 and rear cover 302, slide is equipped in slide chamber 303, is opened on front cover 301
Equipped with the second through-hole being connected to guide pipe 2；The side of front cover 301 offers first annular slot, offered in mounting base 201 with
First annular matched second annular groove of groove shape is equipped with sealing ring 304, sealing ring between first annular slot and the second annular groove
It is bonded with the annular filter paper 305 for absorbing moisture in 304, is detachably connected between mounting base 201 and front cover 301 by buckle
It connects.
As shown in attached drawing 2, attached drawing 3, centrifugal mechanism includes rotor block 4 for connecting with centrifuge 7 and six for pacifying
The centrifugal seat 6 of specimen holder is filled, rotor block 4 is equipped with the bottom plate 5 that cross section is regular hexagon, and rotor block 4 is located at the center of bottom plate 5
On position, the edge of bottom plate 5 and the bottom of centrifugal seat 6 are hinged by hinge, and hinged altogether on bottom plate 5 there are six centrifugal seat 6, rotors
Elastic component is bonded between body 4 and centrifugal seat 6, the elastic component in the present embodiment is preferably tension spring；It is offered in centrifugal seat 6
With the matched special-shaped groove of bottom shape of specimen holder, special-shaped groove includes the slide portion 601 set gradually, guiding pipe portion 602 and sample
Quality control 1, slide portion 601 is expanded far from rotor block 4, the side wall in slide portion 601 from rotor block 4 to the direction of 7 inner wall of centrifuge,
Guiding pipe portion 602 offers the arc groove 603 for picking and placing specimen holder.
It further include the strip groove 701 on 7 inner wall of centrifuge, 701 quantity of strip groove is identical as 6 quantity of centrifugal seat, altogether
There are six strip groove 701, the bottom of strip groove 701 is bonded with spring, and the spring in the present embodiment is preferably tension spring, and spring is remote
One end from strip groove 701 is bonded with the first L-type block 702 that can be slided in strip groove 701, and centrifugal seat 6 is far from rotor block 4
Side wall be equipped with can with the first L-type block 702 disengage the second L-type block 703.
It further include the stator and rotor for being used to form magnetic field, rotor is set on rotor block 4, and stator is set to the interior of centrifuge 7
On wall, the bottom of centrifugal seat 6 is equipped with the conductor for cutting magnetic induction line, is additionally provided with power supply, comparator, relay in centrifugal seat 6
And controller, conductor and comparator form closed circuit, comparator is electrically connected with the controller；As shown in Fig. 4, rear cover 302
Top is set there are two permanent magnet 801, and the distance between two permanent magnets 801 are greater than the width of the fixed slide of slide fixed mechanism
It spends, position corresponding with permanent magnet 801 is equipped with the electromagnet 802 for attracting and repelling permanent magnet 801 on front cover 301, altogether
There are two electromagnet 802；The bottom in slide portion 601 is equipped with protrusion 901, and slide fixed mechanism bottom is equipped with and protrusion
The groove 902 of 901 grafting, protrusion 901 is interior to be equipped with contact, and the sheet metal that can be contacted with contact, protrusion are equipped in groove 902
Contact in 901 is electrically connected to a power source by relay, the sheet metal and electromagnet 802 altogether there are four relay, in groove 902
Conductive winding electrical connection, controller is electrically connected with four relays, and controller is by four relays of control to control
The current direction of the conductive winding of electromagnet 802 processed.
Specific implementation process is as follows:
When using this programme, rear cover 302 is directly broken into two with one's hands, open slide chamber 303, glass slide is placed in slide chamber 303,
It moves rear cover 302 glass slide is pressed on front cover 301, since the permanent magnet 801 on rear cover 302 has magnetism, and front cover 301
Electromagnet 802 in iron core attracted by permanent magnet 801, so that front cover 301 and rear cover 302 be made to clamp glass slide；Pass through rotation
Turn sealing cover and open sample cell 1, sample liquid is added into sample cell 1, after the completion of addition, screwing hermetic lid completes sample liquid
Addition；If needing to change the different annular filter paper 305 of infiltration rate, then before adding sample liquid, break pedestal and front cover 301 into two with one's hands,
The different annular filter paper 305 of bonding infiltration rate in sealing ring 304, then pedestal and front cover 301 are fastened, complete annular filter
The replacement of paper 305.
Centrifugal mechanism is put into centrifuge 7, reviewer pulls out the first L-type block 702 from strip groove 701, and by
Two L-type blocks 703 and the first L-type block 702 fasten, under the collective effect of the first L-type block 702 and elastic component, so that in centrifugal seat 6
Special-shaped groove vertically upward, convenient for placing specimen holder；Specimen holder added with sample liquid is placed in special-shaped groove, slide portion is made
The protrusion 901 of 601 bottoms is inserted into the groove 902 of slide fixed mechanism bottom, so that contact and groove 902 in protrusion 901
In sheet metal be in contact, complete specimen holder and centrifugal seat 6 connection.
The current threshold of comparator is set, starting centrifuge 7 switchs, and rotor block 4 starts to turn under the drive of centrifuge 7
Dynamic, rotor block 4 drives centrifugal seat 6 to rotate, so that the second L-type block 703 and the first L-type block 702 disengage, the first L-type block 702 is in bullet
Under the action of spring, slide into strip groove 701；With the rotation of centrifugal seat 6, generating induced current by conductor cutting magnetic induction line, with
The increase of 6 revolving speed of centrifugal seat, generated induced current increase, compare induced current and current threshold by comparator, work as sense
When electric current being answered to reach current threshold, controller controls two relay conductings, and two relays disconnect, so that power supply is to electromagnet
802 conductive winding is powered, thus magnetize the iron core of electromagnet 802, the magnetic between electromagnet 802 and permanent magnet 801
Power increases, so that front cover 301 and rear cover 302 further clamp glass slide.
In centrifugal process, under the effect of the elastic force of centrifugal force and elastic component, special-shaped groove in centrifugal seat 6 still it is vertical upward；
Sample liquid in sample cell 1 is dumped into guide pipe 2 by first through hole, when the sample liquid in guide pipe 2 passes through the second through-hole,
Moisture in sample liquid is absorbed by annular filter paper 305, so that gathering in the sample liquid on glass slide by the second through-hole
Cell proportion increases.
Centrifuge 7 to be closed to switch, the revolving speed of centrifugal seat 6 reduces, and centrifugal force becomes smaller, under the elastic force effect of elastic component, from
The top of heart seat 6 is tilted to rotor block 4, causes specimen holder also to tilt to rotor block 4, so that the sample liquid in guide pipe 2 is flow backwards
In counter sample quality control 1；As the revolving speed of centrifugal seat 6 reduces, induced current caused by conductor cutting magnetic induction line also reduces therewith, when
When induced current is reduced to current threshold, controller passes through the state for changing relay, so that the relay of conducting is gone off,
The relay of disconnection becomes being connected, thus change the current direction of the conductive winding of electromagnet 802, electromagnet 802 and permanent magnet
Attraction between 801 becomes repulsive force, since the bottom of front cover 301 and rear cover 302 is hinged by hinge, permanent magnet 801 by
To after repulsion, drives rear cover 302 to be turned out, automatically open slide chamber 303, reviewer can be directly from slide chamber 303
Glass slide is taken out, subsequent operation is carried out.
The above are merely the embodiment of the present invention, the common sense such as well known specific structure and characteristic are not made excessively herein in scheme
Description, all common of technical field that the present invention belongs to before one skilled in the art know the applying date or priority date
Technological know-how can know the prior art all in the field, and have using routine experiment means before the date
Ability, one skilled in the art can improve in conjunction with self-ability under the enlightenment that the application provides and implement we
Case, some typical known features or known method should not become the barrier that one skilled in the art implement the application
Hinder.It should be pointed out that for those skilled in the art, without departing from the structure of the invention, if can also make
Dry modification and improvement, these also should be considered as protection scope of the present invention, these all will not influence the effect that the present invention is implemented and
Patent practicability.The scope of protection required by this application should be based on the content of the claims, the specific reality in specification
Applying the records such as mode can be used for explaining the content of claim.
Claims (7)
1. cell centrifugal rotor, including specimen holder and centrifugal mechanism, the specimen holder includes the sample cell for placing sample liquid,
The sample cell is fixedly connected with guide pipe, it is characterised in that: the specimen holder further includes fixing for clamping the slide of slide
Mechanism, the slide fixed mechanism include the hinged front cover and rear cover in bottom, and being equipped between the front cover and the rear cover can
The connector of disengagement has the slide chamber for placing slide between the front cover and the rear cover, the guide pipe is far from sample
One end of pipe is equipped with mounting base, and the mounting base is detachably connected with the front cover, the guide pipe for be connected to sample cell and
Slide chamber；
The centrifugal mechanism includes the rotor block and several centrifugal seats for being used to install specimen holder for connecting with centrifuge, described
It is offered in centrifugal seat and is equipped with bottom plate with the matched special-shaped groove of the bottom shape of specimen holder, the rotor block, the bottom plate
Edge and the bottom of centrifugal seat are hinged, and the rotor block and the centrifugal seat are equipped between the side wall of sample cell makes sample
Pipe is to the inclined elastic component of rotor block.
2. cell centrifugal rotor according to claim 1, it is characterised in that: further include for generating the stator in magnetic field and turning
Son, the centrifugal seat is equipped with the conductor for cutting magnetic induction line, is additionally provided with comparator and controller in the centrifugal seat, described
Conductor and the comparator form closed circuit, and the comparator is electrically connected with controller；The connector includes being set to rear cover
On permanent magnet and the electromagnet on front cover, the controller be used for control the electromagnet conductive winding electric current side
To the special-shaped groove includes slide portion, and the side wall in the slide portion is expanded by rotor block outwardly direction.
3. cell centrifugal rotor according to claim 2, it is characterised in that: the bottom of the slide fixed mechanism with it is described
It is equipped with the protrusion and groove of grafting between slide portion, contact is equipped in the protrusion, being equipped in the groove can connect with contact
The sheet metal of touching.
4. cell centrifugal rotor according to claim 1, it is characterised in that: further include the bar shaped on centrifuge inner wall
Slot, the strip groove are connected with the first L-type block by spring slide, and the centrifugal seat is equipped with close to the side wall of centrifuge inner wall
The the second L-type block that can be disengaged with the first L-type block.
5. cell centrifugal rotor according to claim 1, it is characterised in that: be equipped between the mounting base and the front cover
Sealing ring.
6. cell centrifugal rotor according to claim 5, it is characterised in that: it is extra for absorbing to be equipped in the sealing ring
The annular filter paper of moisture.
7. according to claim 1 or 2 described in any item cell centrifugal rotors, it is characterised in that: the special-shaped groove further includes leading
To pipe portion, the guiding pipe portion offers the arc groove for picking and placing specimen holder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811288703.3A CN109443870B (en) | 2018-10-31 | 2018-10-31 | Cell centrifugal rotor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811288703.3A CN109443870B (en) | 2018-10-31 | 2018-10-31 | Cell centrifugal rotor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109443870A true CN109443870A (en) | 2019-03-08 |
| CN109443870B CN109443870B (en) | 2021-05-28 |
Family
ID=65550447
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811288703.3A Active CN109443870B (en) | 2018-10-31 | 2018-10-31 | Cell centrifugal rotor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109443870B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022062376A1 (en) * | 2020-09-25 | 2022-03-31 | 南通纺织丝绸产业技术研究院 | Cell culture device with micro-current stimulation function |
| CN114506946A (en) * | 2022-04-15 | 2022-05-17 | 深圳市世邦环境科技有限公司 | Closed-loop disinfection and deodorization device for volatile waste water body |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022062376A1 (en) * | 2020-09-25 | 2022-03-31 | 南通纺织丝绸产业技术研究院 | Cell culture device with micro-current stimulation function |
| CN114506946A (en) * | 2022-04-15 | 2022-05-17 | 深圳市世邦环境科技有限公司 | Closed-loop disinfection and deodorization device for volatile waste water body |
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| Publication number | Publication date |
|---|---|
| CN109443870B (en) | 2021-05-28 |
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