CN109443870B

CN109443870B - Cell centrifugal rotor

Info

Publication number: CN109443870B
Application number: CN201811288703.3A
Authority: CN
Inventors: 陈中奇
Original assignee: Chongqing Yingteli Technology Co ltd
Current assignee: Chongqing Yingteli Technology Co ltd
Priority date: 2018-10-31
Filing date: 2018-10-31
Publication date: 2021-05-28
Anticipated expiration: 2038-10-31
Also published as: CN109443870A

Abstract

The invention relates to the technical field of medical instruments, in particular to a cytocentrifugal rotor, which comprises a sample seat and a centrifugal mechanism, wherein the sample seat comprises a sample tube for placing sample liquid, the sample tube is fixedly connected with a guide tube, the sample seat also comprises a slide fixing mechanism for clamping a slide, and the guide tube is used for communicating the sample tube with a slide cavity; the centrifugal mechanism comprises a rotor body and a plurality of centrifugal seats, the rotor body is used for being connected with the centrifugal machine, the centrifugal seats are used for installing the sample seats, special-shaped grooves matched with the bottom shapes of the sample seats are formed in the centrifugal seats, a bottom plate is arranged on the rotor body, the edge of the bottom plate is hinged with the bottom of the centrifugal seats, and elastic pieces enabling the sample tubes to incline towards the rotor body are arranged between the rotor body and the side wall, close to the sample tubes, of the centrifugal seats. This scheme of adoption can prevent that the sample liquid drop in the stand pipe from falling to slide fixed establishment, can be when taking out the slide, does not scratch the cell on slide surface.

Description

Cell centrifugal rotor

Technical Field

The invention relates to the technical field of medical instruments, in particular to a cell centrifugal rotor.

Background

In the process of preparing or culturing cells, it is generally important to centrifuge the sample solution, separate various components of the sample solution by centrifugation to obtain desired components for study, or aggregate dispersed cells by centrifugation to aid in the screening of the cells.

The cell centrifuge comprises a cell centrifuge main body and a cell centrifuge rotor, wherein the cell centrifuge main body provides centrifugal power, the cell centrifuge rotor is arranged on the cell centrifuge main body, a sample cavity for placing sample liquid is arranged on the cell centrifuge rotor, and the cell centrifuge rotor can be replaced to place the sample liquid with various requirements in the cell centrifuge main body.

Current cytocentrifuge rotor is including placing sample chamber and the slide fixed establishment of specimen slide liquid, and the slide among the slide fixed establishment passes through the pipeline with the sample chamber and communicates, and slide fixed establishment gets through the mode of inserting, extracting and puts the slide, gets to put inconveniently, scrapes easily when taking out the slide and rubs the smear position for the cell of smear position is scraped, influences the inspection result.

Secondly, when the glass slide is taken out, the sample liquid still exists in the sample cavity and the pipeline, and as the glass slide is taken out, the sample liquid in the pipeline may flow into the glass slide fixing mechanism, so that the sample liquid drops into the detection environment, and if the detected sample liquid is a substance with infectivity, the detection environment is polluted, and the risk of spreading diseases exists.

Disclosure of Invention

The invention aims to provide a cell centrifugal rotor, which can prevent sample liquid in a guide pipe from dropping into a slide fixing mechanism and can prevent cells on the surface of a slide from being scraped when the slide is taken out.

The basic scheme provided by the invention is as follows: the cell centrifugal rotor comprises a sample seat and a centrifugal mechanism, wherein the sample seat comprises a sample tube for placing sample liquid, the sample tube is fixedly connected with a guide tube, the sample seat also comprises a slide fixing mechanism for clamping a slide, the slide fixing mechanism comprises a front cover and a rear cover which are hinged at the bottoms, a connecting piece capable of being separated is arranged between the front cover and the rear cover, a slide cavity for placing the slide is arranged between the front cover and the rear cover, a mounting seat is arranged at one end of the guide tube far away from the sample tube, the mounting seat is detachably connected with the front cover, and the guide tube is used for communicating the sample tube with the slide cavity; the centrifugal mechanism comprises a rotor body and a plurality of centrifugal seats, the rotor body is used for being connected with the centrifugal machine, the centrifugal seats are used for installing the sample seats, special-shaped grooves matched with the bottom shapes of the sample seats are formed in the centrifugal seats, a bottom plate is arranged on the rotor body, the edge of the bottom plate is hinged with the bottom of the centrifugal seats, and elastic pieces enabling the sample tubes to incline towards the rotor body are arranged between the rotor body and the side wall, close to the sample tubes, of the centrifugal seats.

The principle and the advantages of the invention are as follows:

1. because the edge of bottom plate is articulated with the bottom of centrifugation seat, all be equipped with the elastic component that makes the sample cell to the rotor block slope between the lateral wall that rotor block and centrifugation seat are close to the sample cell, under quiescent condition, the distance of centrifugation seat bottom to rotor block is certain, receive the pulling force of elastic component, make centrifugation seat top to the rotor block slope, because the sample seat is installed in the centrifugation seat, the centrifugation seat slope leads to the sample seat also to take place the slope, make the oral area that the stand pipe is close to the slide chamber be higher than the oral area that is close to the sample chamber, the sample liquid among the stand pipe flows back to in the sample chamber, thereby prevent that the sample liquid in the stand pipe from dropping to the slide chamber of slide fixed establishment.

2. Because be equipped with the connecting piece that can throw off between protecgulum and the back lid, the protecgulum is articulated with the bottom of back lid, and the connecting piece is thrown off the back, through breaking the protecgulum with the back lid off with the fingers and thumb, opens the slide chamber, takes out the slide along the back lid, and the in-process of taking out, the smear position of slide can not contact with the protecgulum, when taking out the slide, does not scratch the cell on slide surface.

The centrifugal machine further comprises a stator and a rotor which are used for generating a magnetic field, a conductor which is used for cutting a magnetic induction line is arranged on the centrifugal seat, a comparator and a controller are further arranged on the centrifugal seat, the conductor and the comparator form a closed loop, and the comparator is electrically connected with the controller; the connecting piece covers the permanent magnet and locates the electro-magnet on the protecgulum after locating, and the controller is used for controlling the current direction of the conductive winding of electro-magnet, and heterotypic groove includes the slide portion, and the lateral wall of slide portion is expanded by the outside direction of rotor block.

A magnetic field is formed in the centrifuge through the stator and the rotor, because the centrifuge seat is provided with a conductor for cutting a magnetic induction line, the centrifuge seat rotates by taking the rotor body as a shaft in the centrifugation process, the conductor cuts the magnetic induction line of the magnetic field to generate an induced current, the induced current is in direct proportion to the rotating speed of the centrifuge seat, when the centrifuge is started, the rotating speed is gradually increased until reaching a stable rotating speed and is kept stable, when the centrifuge is stopped, the rotating speed is gradually reduced until stopping, a current threshold value is set, whether the induced current reaches the current threshold value is compared through a comparator, when the induced current reaches the current threshold value for the first time, the centrifuge is in a starting state, the controller controls the conductive winding to be electrified, the electromagnet attracts the permanent magnet to further clamp the slide by the front cover and the rear cover, when the induced current reaches the current threshold value for the second time, the centrifuge, the electromagnet repels the permanent magnet, so that the back cover automatically opens the slide cavity, the slide can be conveniently taken out, and cells on the surface of the slide cannot be scraped.

Furthermore, a protrusion and a groove which are connected in an inserting mode are arranged between the bottom of the glass slide fixing mechanism and the glass slide portion, a contact is arranged in the protrusion, and a metal sheet which can be contacted with the contact is arranged in the groove.

Because be equipped with the arch and the recess of pegging graft between slide fixed establishment's bottom and the slide portion, when installing the sample seat at centrifugal seat, can fix the position of sample seat through arch and recess, secondly, contact mutually through the contact in the arch and the sheetmetal in the recess and can switch on the conductive winding of electro-magnet.

Further, still including locating the bar groove on the centrifuge inner wall, the bar groove has first L type piece through spring sliding connection, is equipped with the second L type piece that can throw off with first L type piece on the lateral wall that centrifugal seat is close to the centrifuge inner wall.

The edge of the bottom plate is hinged with the bottom of the centrifugal seat, the elastic parts for enabling the sample tube to incline towards the rotor body are arranged between the rotor body and the side wall of the centrifugal seat close to the sample tube, the centrifugal seat can incline under the action of the elastic parts, and the centrifugal seat is kept in a vertical state through the matching of the first L-shaped block and the second L-shaped block, so that the sample seat can be conveniently placed into the centrifugal seat; when the centrifugal seat rotates, the first L-shaped block is separated from the second L-shaped block, and the first L-shaped block is connected into the strip-shaped groove in a sliding mode through the spring and is pulled into the strip-shaped groove under the action of the spring, so that the second L-shaped block is prevented from colliding with the first L-shaped block in the rotating process.

Further, a sealing ring is arranged between the mounting seat and the front cover.

Make mount pad and protecgulum further sealed through the sealing washer, prevent that sample liquid from dripping from the gap between mount pad and the protecgulum.

Furthermore, the sealing ring is internally provided with annular filter paper for absorbing redundant moisture.

Sample liquid can isolate moisture through the centrifugation, moisture is along with the cell is together through the stand pipe flow to the slide chamber, thereby carry out the smear, when moisture is too much can lead to the smear, the cell of gathering is too few on the slide, be unfavorable for observing, because be equipped with in the sealing washer and be used for absorbing the annular filter paper of unnecessary moisture, when sample liquid flows through annular filter paper, moisture in the sample liquid is absorbed by annular filter paper, make the cell volume of gathering on the slide increase, be favorable to observing the cell.

Furthermore, the abnormal shape groove still includes the stand pipe portion, and the stand pipe portion is seted up and is used for getting the arc wall of putting the sample seat.

Because the arc wall has been seted up to the stand pipe portion, after the centrifugation is accomplished, can conveniently take out the sample seat from the centrifugation seat through the arc wall.

Drawings

FIG. 1 is a partial cross-sectional view of a sample holder of an embodiment of a cytospin rotor of the present invention.

FIG. 2 is a cross-sectional view of a centrifuge bowl of an embodiment of a cytospin rotor of the present invention.

FIG. 3 is a top view of a centrifuge mechanism according to an embodiment of the present invention.

FIG. 4 is an enlarged view of the portion A of FIG. 1 according to the embodiment of the present invention.

Detailed Description

The following is further detailed by way of specific embodiments:

reference numerals in the drawings of the specification include: the device comprises a sample tube 1, a guide tube 2, a mounting seat 201, a front cover 301, a rear cover 302, a slide cavity 303, a sealing ring 304, annular filter paper 305, a rotor body 4, a bottom plate 5, a centrifugal seat 6, a slide part 601, a guide tube part 602, an arc-shaped groove 603, a centrifugal machine 7, a strip-shaped groove 701, a first L-shaped block 702, a second L-shaped block 703, a permanent magnet 801, an electromagnet 802, a protrusion 901 and a groove 902.

Examples

The cell centrifugal rotor comprises a sample seat and a centrifugal mechanism, as shown in figure 1, the sample seat comprises a sample tube 1 for placing sample liquid, a sealing cover is in threaded connection with an opening of the sample tube 1, the bottom of the sample tube 1 is arc-shaped, a first through hole is formed in the side wall of the sample tube 1, a guide tube 2 is bonded to the first through hole, and a mounting seat 201 is arranged at one end, far away from the first through hole, of the guide tube 2; the sample holder also comprises a slide fixing mechanism for clamping a slide, the slide fixing mechanism comprises a front cover 301 and a rear cover 302, the bottoms of the front cover 301 and the rear cover 302 are hinged through a hinge, a slide cavity 303 is arranged between the front cover 301 and the rear cover 302, a slide is arranged in the slide cavity 303, and a second through hole communicated with the guide pipe 2 is formed in the front cover 301; first ring channel has been seted up to the side of protecgulum 301, has seted up the second ring channel that matches with first ring channel shape on the mount pad 201, is equipped with sealing washer 304 between first ring channel and the second ring channel, and the bonding has the annular filter paper 305 that is used for absorbing moisture in the sealing washer 304, can dismantle through the buckle between mount pad 201 and the protecgulum 301 and be connected.

As shown in fig. 2 and fig. 3, the centrifugal mechanism includes a rotor body 4 connected to a centrifuge 7 and six centrifugal seats 6 for mounting sample seats, a bottom plate 5 with a regular hexagonal cross section is disposed on the rotor body 4, the rotor body 4 is located at the center of the bottom plate 5, the edge of the bottom plate 5 is hinged to the bottom of the centrifugal seat 6 through a hinge, the bottom plate 5 is hinged to six centrifugal seats 6, elastic members are bonded between the rotor body 4 and the centrifugal seats 6, and the elastic members in this embodiment are preferably tension springs; the heterotypic groove that matches with the bottom shape of sample holder has all been seted up on centrifugal seat 6, and heterotypic groove is including the slide portion 601, guide tube portion 602 and the sample cell 1 portion that set gradually, and rotor body 4 is kept away from to slide portion 601, and the lateral wall of slide portion 601 is expanded by rotor body 4 to the direction of centrifuge 7 inner wall, and guide tube portion 602 offers the arc wall 603 that is used for getting to put the sample holder.

The centrifugal machine further comprises a strip-shaped groove 701 arranged on the inner wall of the centrifugal machine 7, the number of the strip-shaped grooves 701 is the same as that of the centrifugal seats 6, six strip-shaped grooves 701 are formed in total, springs are bonded to the bottoms of the strip-shaped grooves 701, the springs in the embodiment are preferably tension springs, a first L-shaped block 702 capable of sliding in the strip-shaped grooves 701 is bonded to one end, far away from the strip-shaped grooves 701, of each spring, and a second L-shaped block 703 capable of being disengaged from the first L-shaped block 702 is arranged on the side wall, far away from the rotor.

The centrifugal machine also comprises a stator and a rotor which are used for forming a magnetic field, wherein the rotor is arranged on the rotor body 4, the stator is arranged on the inner wall of the centrifugal machine 7, a conductor which is used for cutting a magnetic induction line is arranged at the bottom of the centrifugal seat 6, a power supply, a comparator, a relay and a controller are further arranged on the centrifugal seat 6, the conductor and the comparator form a closed loop, and the comparator is electrically connected with the controller; as shown in fig. 4, two permanent magnets 801 are arranged on the top of the back cover 302, the distance between the two permanent magnets 801 is larger than the width of the slide fixed by the slide fixing mechanism, an electromagnet 802 for attracting and repelling the permanent magnets 801 is arranged on the front cover 301 corresponding to the permanent magnets 801, and the two electromagnets 802 are provided in total; the bottom of slide portion 601 all is equipped with protruding 901, all be equipped with the recess 902 of pegging graft with protruding 901 of slide fixed establishment bottom, be equipped with the contact in the protruding 901, be equipped with in the recess 902 can with the sheetmetal of contact, the contact in the protruding 901 passes through the relay and is connected with the power electricity, total four relays, sheetmetal in the recess 902 is connected with electro-magnet 802's conductive winding electricity, the controller is connected with four relays electricity respectively, thereby the controller is through the current direction of controlling four relays control electro-magnet 802's conductive winding.

The specific implementation process is as follows:

when the scheme is adopted, the rear cover 302 is directly opened, the glass slide cavity 303 is opened, the glass slide is placed in the glass slide cavity 303, the rear cover 302 is pulled to press the glass slide on the front cover 301, and the front cover 301 and the rear cover 302 clamp the glass slide because the permanent magnet 801 on the rear cover 302 has magnetism and the iron core in the electromagnet 802 of the front cover 301 is attracted by the permanent magnet 801; opening the sample tube 1 by rotating the sealing cover, adding the sample liquid into the sample tube 1, and screwing the sealing cover after the addition is finished to finish the addition of the sample liquid; if the annular filter paper 305 with different absorption speeds needs to be replaced, before adding the sample liquid, the base and the front cover 301 are broken, the annular filter paper 305 with different absorption speeds is adhered in the sealing ring 304, and then the base and the front cover 301 are buckled, so that the replacement of the annular filter paper 305 is completed.

Placing the centrifugal mechanism into a centrifuge 7, pulling out a first L-shaped block 702 from a strip-shaped groove 701 by an inspector, buckling a second L-shaped block 703 with the first L-shaped block 702, and enabling a profiled groove on the centrifugal seat 6 to be vertically upward under the combined action of the first L-shaped block 702 and an elastic piece so as to be convenient for placing a sample seat; the sample holder added with the sample liquid is placed in the special-shaped groove, the bulge 901 at the bottom of the glass sheet part 601 is inserted into the groove 902 at the bottom of the glass sheet fixing mechanism, so that the contact in the bulge 901 is contacted with the metal sheet in the groove 902, and the connection of the sample holder and the centrifugal seat 6 is completed.

Setting a current threshold value of the comparator, starting a switch of a centrifugal machine 7, enabling a rotor body 4 to start to rotate under the driving of the centrifugal machine 7, enabling the rotor body 4 to drive a centrifugal seat 6 to rotate, enabling a second L-shaped block 703 to be separated from a first L-shaped block 702, and enabling the first L-shaped block 702 to slide into a strip-shaped groove 701 under the action of a spring; along with the rotation of the centrifugal seat 6, the conductor cuts the magnetic induction line to generate induction current, along with the increase of the rotating speed of the centrifugal seat 6, the generated induction current is increased, the induction current is compared with a current threshold value through the comparator, when the induction current reaches the current threshold value, the controller controls the two relays to be switched on and switched off, so that the power supply supplies power to the conductive winding of the electromagnet 802, the iron core of the electromagnet 802 is magnetized, the magnetic force between the electromagnet 802 and the permanent magnet 801 is increased, and the front cover 301 and the rear cover 302 further clamp the glass slide.

In the centrifugal process, the special-shaped groove on the centrifugal seat 6 is still vertically upward under the action of centrifugal force and the elastic force of the elastic piece; the sample liquid in the sample tube 1 is thrown into the guide tube 2 through the first through hole, and when the sample liquid in the guide tube 2 passes through the second through hole, the moisture in the sample liquid is absorbed by the annular filter paper 305, so that the proportion of cells in the sample liquid collected on the slide glass through the second through hole is increased.

The switch of the centrifuge 7 is closed, the rotating speed of the centrifugal seat 6 is reduced, the centrifugal force is reduced, and under the elastic action of the elastic part, the top of the centrifugal seat 6 inclines towards the rotor body 4, so that the sample seat also inclines towards the rotor body 4, and the sample liquid in the guide tube 2 flows back to the sample tube 1; the induced current generated by the conductor cutting magnetic induction line is reduced along with the reduction of the rotating speed of the centrifugal seat 6, when the induced current is reduced to a current threshold value, the controller changes the state of the relay to turn off the switched-on relay and turn on the switched-off relay, so that the current direction of the conductive winding of the electromagnet 802 is changed, the attractive force between the electromagnet 802 and the permanent magnet 801 is changed into repulsive force, the front cover 301 and the bottom of the rear cover 302 are hinged through a hinge, the permanent magnet 801 is repelled to drive the rear cover 302 to rotate outwards, the slide cavity 303 is automatically opened, and an inspector can directly take out a slide from the slide cavity 303 to perform subsequent operation.

The foregoing are merely exemplary embodiments of the present invention, and no attempt is made to show structural details of the invention in more detail than is necessary for the fundamental understanding of the art, the description taken with the drawings making apparent to those skilled in the art how the several forms of the invention may be embodied in practice with the teachings of the invention. It should be noted that, for those skilled in the art, without departing from the structure of the present invention, several changes and modifications can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.

Claims

1. Cell centrifugation rotor, including sample seat and centrifugal mechanism, the sample seat is including being used for placing the sample cell of sample liquid, sample cell fixedly connected with stand pipe, its characterized in that: the sample holder also comprises a slide fixing mechanism for clamping a slide, the slide fixing mechanism comprises a front cover and a rear cover which are hinged at the bottoms, a detachable connecting piece is arranged between the front cover and the rear cover, a slide cavity for placing the slide is arranged between the front cover and the rear cover, one end of the guide tube, which is far away from the sample tube, is provided with a mounting seat, the mounting seat is detachably connected with the front cover, and the guide tube is used for communicating the sample tube with the slide cavity;

the centrifugal mechanism comprises a rotor body and a plurality of centrifugal seats, the rotor body is used for being connected with a centrifugal machine, the centrifugal seats are used for mounting a sample seat, special-shaped grooves matched with the bottom of the sample seat in shape are formed in the centrifugal seats, a bottom plate is arranged on the rotor body, the edge of the bottom plate is hinged with the bottom of the centrifugal seats, and elastic pieces enabling the sample tubes to incline towards the rotor body are arranged between the rotor body and the side walls, close to the sample tubes, of the centrifugal seats;

the centrifugal machine also comprises a stator and a rotor which are used for generating a magnetic field, wherein a conductor which is used for cutting a magnetic induction line is arranged on the centrifugal seat, a comparator and a controller are also arranged on the centrifugal seat, the conductor and the comparator form a closed loop, and the comparator is electrically connected with the controller; the connecting piece comprises a permanent magnet arranged on the rear cover and an electromagnet arranged on the front cover, the controller is used for controlling the current direction of a conductive winding of the electromagnet, the special-shaped groove comprises a glass sheet part, and the side wall of the glass sheet part is expanded in the outward direction of the rotor body;

the centrifugal seat is arranged on the centrifugal machine, the conductor cuts the magnetic induction line to generate induced current, the induced current is in direct proportion to the rotating speed of the centrifugal seat, the comparator is used for comparing the induced current with the current threshold, when the induced current reaches the current threshold, the controller controls the conduction of the conductive winding, the electromagnet attracts the permanent magnet, and when the induced current reaches the current threshold again, the controller changes the current direction of the conductive winding, and the electromagnet repels the permanent magnet.

2. The cytocentrifuge rotor of claim 1, wherein: the bottom of slide fixed establishment with be equipped with the arch and the recess of grafting between the slide portion, be equipped with the contact in the arch, be equipped with the sheetmetal that can contact with the contact in the recess.

3. The cytocentrifuge rotor of claim 1, wherein: still including locating the bar groove on the centrifuge inner wall, the bar groove has first L type piece through spring sliding connection, be equipped with on the lateral wall that the centrifugal seat is close to the centrifuge inner wall and can with first L type piece detached second L type piece.

4. The cytocentrifuge rotor of claim 1, wherein: and a sealing ring is arranged between the mounting seat and the front cover.

5. The cytocentrifuge rotor of claim 4, wherein: and annular filter paper for absorbing redundant moisture is arranged in the sealing ring.

6. The cytocentrifuge rotor of claim 1, wherein: the special-shaped groove further comprises a guide pipe part, and the guide pipe part is provided with an arc-shaped groove used for taking and placing the sample seat.