JP2009153387A - Cell-washing centrifugal machine - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a cell-washing centrifugal machine having good maintainability without making a drain liquid which slips and collects into a clearance between a body chamber and a door cover flow down into a part other than the chamber, and stain the periphery of an upper part of a cashing when the door is opened after a washing treatment. <P>SOLUTION: The cell-washing centrifugal machine includes a motor which is a driving source, a rotor, a plurality of test tube holders freely rotatably mounted in a circular row on the rotor, a wash liquid distribution element for feeding the wash liquid into the interior of the plurality of test tubes held with the test tube holders, the body chamber 17 for covering the periphery of the rotor, the test tube holders, and the wash liquid distribution element, the door cover 18 for covering the upper part of the body chamber 17, and a cover packing 19 mounted on the outer periphery of the door cover 18 and hermetically closing the clearance between the body chamber 17 and the door cover 18. In the cell-washing centrifugal machine, a cylindrical space S is formed between the inner peripheral surface of the cover packing 19 and the outer peripheral surface of the door cover 18. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to a cell washing centrifuge for washing biological cells such as red blood cells using centrifugal force.

  Conventionally, cell washing centrifuges have been used to wash erythrocytes in blood with a washing solution such as physiological saline in antiglobulin tests, cross-match tests, irregular antibody screenings, etc. at the time of blood transfusion testing. Is used to remove.

  This type of cell washing centrifuge is equipped with a motor that is a driving source, a rotor that is driven to rotate by the motor, a plurality of test tube holders that are rotatably mounted in a circular row on the rotor, and a rotor that is mounted on the rotor. And a magnetic element for attracting and holding the test tube holder at a vertical angle or a near vertical angle by a magnetic attraction generated by energizing the magnetic coil.

  Here, the test tube holder is a magnetic member that holds the test tube, rotates together with the rotor, rotates in the outer peripheral horizontal direction by centrifugal force, contacts the bowl rotating with the rotor, and It rotates while being held at a predetermined angle. The cleaning liquid distribution element rotates with the rotor and supplies the cleaning liquid to the inside of the test tube held by the test tube holder.

  By the way, Patent Document 1 discloses a cleaning liquid distribution element of a cleaning centrifuge, and this cleaning liquid distribution element has nozzles that are radially installed from the outer periphery of the bottom surface of a conical container. By rotating together with the rotor, the cleaning liquid injected from the center is equally divided, and the cleaning liquid is supplied from the nozzle into the plurality of test tubes held in the test tube holder.

  Patent Document 2 discloses a configuration in which a hole is formed in the cleaning liquid distribution element that rotates together with the rotor, and the cleaning liquid is supplied from the hole into each test tube held by the test tube holder. Further, Patent Document 2 also discloses a configuration in which a test tube holder is held by a rotor using a magnetic element.

  Further, in Patent Documents 3 and 4, the test tube holder is rotated and driven at a low speed while holding the test tube holder at a small angle with respect to the vertical direction using a rim or a rotating member. Discharging technology is disclosed.

  Further, in Patent Document 5, the test tube holder is held on the rotor by a magnetic element in a state inclined at a smaller angle than the vertical direction, and the cleaning liquid is removed from the test tube held in the test tube holder while rotating the rotor at a low speed. A technique for discharging the supernatant liquid is disclosed.

On the other hand, as a cell washing centrifuge, an automatic blood cell washing centrifuge that automatically executes a washing process including a washing liquid injection process, a centrifugation process, a supernatant discharge process, and a rocking process in order is known. For example, as shown in Non-Patent Document 1, an automatic blood cell washing centrifuge sold by the applicant under the product name “himacMC450” is well known. The washing process using this automatic blood cell washing centrifuge is executed by the following procedure.
(1) First, as a preparation, the door of an automatic blood cell washing centrifuge is opened, a test tube containing biological cells such as blood cells is set in a test tube holder of a rotor, and then the door is closed. Normally, 24 test tubes containing biological cells are generally used. However, when the number of specimens is small, the number of test tubes is set to 12 and the test tube holder is tested so that the balance of the rotor is not lost. Other tubes may be set and washed.
(2) Next, as a cleaning liquid injection step, the rotor is accelerated and rotated by a motor, the lower part of the test tube in the test tube holder is rotated outward by the centrifugal force, and the test tube is moved from the vertical direction to the central axis of the rotor. The rotor (motor) is rotated so as to be inclined at a certain angle. At this time, the cleaning liquid is injected into the test tube via the cleaning liquid distribution element that rotates together with the rotor by turning the liquid feeding pump operation on (ON) (power supplied to the pump). Then, the blood cells are stirred and washed by the moment of washing liquid injection.
(3) Next, in the centrifugation step, for example, the rotor (motor) is rotated at 3000 rpm for 45 seconds and centrifuged. As a result, blood cells settle to the bottom of the test tube, and unnecessary substances such as serum remain in the supernatant.
(4) Next, in the supernatant discharge process, energization of the magnetic coil is turned on, the magnetic element operation is turned on, and the test tube holder is made substantially vertical by the attractive force generated in the magnetic element. Adsorb and fix in a state or an open state at a small angle upward. When the rotor is rotated again at a low speed of, for example, 400 rpm in this state, the upper part of the test tube is opened at a small angle or is in a vertical state, so that the supernatant liquid rises up the wall surface of the test tube by centrifugal force to the outside. Discharged. When the rotation of the rotor (motor) is immediately stopped, only the precipitated blood cells remain in the test tube.
(5) Next, in the swinging process, the rotation and stop of the rotor are alternately repeated in small increments, or the forward and reverse rotations of the rotor are alternately repeated in small increments to obtain a test tube held in the test tube holder. Apply rocking to allow blood cells to settle at the bottom of the test tube and unfasten the blood cells.
(6) The above steps (2) to (5) are set as one cycle of white bulb washing, and this cycle is usually repeated 3 to 4 times, and then the automatic blood cell washing centrifuge door is opened and the test tube is taken out. Complete the washing by.

  Here, a sealing structure between a main chamber and a door cover of a conventional cell washing centrifuge will be described with reference to FIGS.

  FIG. 7 is a partial cross-sectional view showing a sealed state of the main chamber and the door cover by cover packing of a conventional cell washing centrifuge, and FIG. 8 is a partial perspective view of the opened door as seen from the inside. As described above, the inner surface of the door 103 is covered with a main body chamber 117 around the rotor (not shown) accommodated in the housing 102, the plurality of test tube holders supported by the rotor, and the cleaning liquid distribution element. A door cover 118 that covers the upper portion of the chamber 117 is provided on the inner surface of the door 103, and a cover packing 119 formed in a ring shape by an elastic body such as rubber is fitted on the outer periphery of the door cover 118. It is worn.

As shown in FIG. 7, the cover packing 119 is formed in a C-shaped cross section that opens outward, and when the door 102 is closed as shown in the drawing, the cover packing 119 opens to the upper surface of the chamber 117. The inside of the chamber 117 is sealed in close contact with the peripheral edge of the portion, and liquid leakage from the chamber 117 is surely prevented.
JP 50-022693 A Japanese Utility Model Publication No. 2-081640 Japanese Patent Publication No. 48-027267 JP 60-150857 A Japanese Utility Model Publication No. 54-167860 Hitachi Koki Co., Ltd. Website “Hitachi Automatic Blood Cell Washing Machine MC450”, <URL: http://www.hitachi-koki.co.jp/himac/products/centrifuges/smal_centrifuges/mc450/mc450.html>

  In the conventional cell washing centrifuge described above, when the number of test tubes is, for example, 12 that is 1/2 of the normal number, the test solution does not exist and thus the cleaning liquid is discharged as it is into the main body chamber 117 and accumulated at the bottom. In some cases, or when the liquid discharge port is closed due to some circumstances of the operating operator and the discharged supernatant is difficult to be discharged, the cleaning liquid or discharged supernatant (hereinafter referred to as the discharged supernatant) accumulated at the bottom of the main body chamber 117 is used. These are collectively referred to as “drain liquid”) and rotate around the rotor along the bottom of the main body chamber 117 by the air flow generated by the rotation of the rotor, test tube holder, and cleaning liquid distribution element. The flow of the drain liquid becomes turbulent when the bottom and inner peripheral portions of the main body chamber 117 are uneven, and soars toward the door cover 118 provided at a position facing the main body chamber 117. Then, the drain liquid soared adheres to the inner peripheral wall of the main body chamber 117 and the outer peripheral surface of the door cover 118 in the form of mist or droplets, and enters the gap between the main body chamber 117 and the door cover 118. Accumulate in liquid form.

  Incidentally, in the rotary chamber 120 of the conventional cell washing centrifuge shown in FIG. 7, in the sealed structure of the removable chamber 117 and the door cover 118, when the door 102 is opened after the washing process is completed, The drain liquid adhering to the surface does not drip as long as it moves due to the vibration applied to the door cover 118, but the drain liquid accumulated in the liquid by sinking into the gap between the chamber 117 and the outer surface 108 a of the door cover 118 The door cover 118 drips due to the lifted momentum, hangs down along the sealing surface of the cover packing 119, and the drain liquid falls to a portion other than the chamber upper portion 107a, and the surroundings of the upper surface 102a of the casing are soiled. May occur.

  In addition, when it is not parallel to the sealing surface 117b that contacts the sealing surface of the cover packing 119 of the chamber upper portion 117a, the drain liquid that has entered the gap between the chamber 117 and the door cover 118 and accumulated as a liquid is covered. Even if there is little drain liquid flowing from the door cover 118 when the door 102 is opened after completion of the cleaning process, the cover packing 119 can be sealed without being completely adhered between the packing 119 and the chamber 117. There is a possibility that the drain droplets adhering to the surface flow down along the sealing surface of the cover packing 119 and the drain liquid falls to a portion other than the chamber 117, and the surroundings of the housing upper surface 102a may be soiled.

  The present invention has been made in view of the above problems, and the intended process is that the drain liquid that has entered the gap between the main body chamber and the door cover is accumulated in a portion other than the chamber when the door is opened after the cleaning process. The object of the present invention is to provide a cell washing centrifuge with good maintainability that does not flow down and stain around the upper part of the housing.

  In order to achieve the above object, the invention described in claim 1 is a motor as a drive source, a rotor driven to rotate by the motor, and a plurality of test tubes rotatably mounted in a circular row on the rotor. A holder, a cleaning liquid distribution element for supplying a cleaning liquid into a plurality of test tubes held by the test tube holder, holding means for holding the test tube holder at a vertical or near vertical angle, the rotor, and the test tube holder And a main body chamber that covers the periphery of the cleaning liquid distribution element, a door cover that covers the upper portion of the main body chamber, and a cover packing that is fitted to the outer periphery of the door cover and seals between the main body chamber and the door cover. In the cell washing centrifuge, a cylindrical space is formed between an inner peripheral surface of the cover packing and an outer peripheral surface of the door cover.

  The invention according to claim 2 is characterized in that, in the invention according to claim 1, the thickness of the support portion of the cover packing is made thinner than the thickness of the lip portion to give the support portion elasticity.

  According to a third aspect of the present invention, in the second aspect of the present invention, the support portion that supports the lip portion of the cover packing is offset toward the door cover side with respect to the center in the width direction of the cover packing mounting portion. And

  According to the first aspect of the present invention, since the cylindrical space is formed between the inner peripheral surface of the cover packing and the outer peripheral surface of the door cover, the liquid enters the gap between the main body chamber and the door cover and accumulates as a liquid. When the drain liquid drips due to the momentum of lifting the door cover when opening the door, the inner surface of the cover packing provided to create a space between the cover packing and the door cover acts as a barrier on the sealing surface. The drain liquid does not flow, and the drain liquid flows along the outer periphery of the door cover along the groove formed by the space between the cover packing and the door cover, and falls to a predetermined position provided in the main body chamber. For this reason, the liquid dripping to the upper part of a housing is prevented, the circumference | surroundings are not polluted by drain liquid, and high reliability is ensured for a cell washing centrifuge.

  According to the second aspect of the present invention, the thickness of the support part of the cover packing is made thinner than the thickness of the lip part so that the support part has elasticity. Even if it is slightly non-parallel, the sealing surface of the cover packing and the sealing surface of the main body chamber are likely to be parallel to improve the sealing performance, and the cover packing and the main chamber are not completely in close contact with each other. The amount of drain liquid that has entered the space on the sealed surface is reduced, and the amount of drain liquid that adheres to the sealed surface of the cover packing is reduced, so that dripping of drain droplets can be eliminated.

  According to the third aspect of the present invention, the support portion that supports the lip portion of the cover packing is offset toward the door cover side with respect to the center in the width direction of the cover packing mounting portion. A space can be formed, the amount of drain liquid accumulated between the cover packing and the door cover increases, and the flow of drain liquid flows along the groove created by the space between the cover packing and the door cover when the door is opened. Is prioritized, and the flow of drain liquid to the sealing surface of the lip portion can be eliminated.

  Embodiments of the present invention will be described below with reference to the accompanying drawings.

  FIG. 1 is a cross-sectional view showing the overall configuration of a cell washing centrifuge according to the present invention, and FIG. 2 is a cross-sectional view of a main part showing an operating state of a test tube holder in each washing process of the cell washing centrifuge.

  As shown in FIG. 1, the cell washing centrifuge 1 according to the present invention includes a rectangular box-shaped casing (frame) 2 and a door 3 that opens and closes the upper portion of the casing 2. Includes a motor 5 having a drive shaft (rotating shaft) 4, and a housing 2 is provided with a rotating chamber 20, and extends to the upper portion of the housing 2 inside the rotating chamber 20. A removable chamber 17 having a chamber top 17a is arranged. A rotor 6 that is connected to the drive shaft 4 of the motor 5 and is driven to rotate is incorporated in a space partitioned by the chamber 17 of the rotation chamber 20 and the rotor chamber 21. Furthermore, a plurality of (for example, 24) test tube holders 7 are arranged in a circular array on the rotor 6 in a plan view, and each test tube holder 7 can be rotated in the vertical direction on the outer periphery of the rotor 6. It is attached to. Each test tube holder 7 holds a test tube 8 in which a suitable amount of living cells such as red blood cells are stored in advance. The test tube holder 7 is made of a magnetic material such as stainless steel (SUS).

  Further, the cell washing centrifuge 1 includes a magnetic element 9 for attracting the test tube holder 7 by magnetic force as a holding means for holding the test tube holder 7 at a small angle close to or perpendicular to the rotor 6. Yes. The magnetic element 9 includes a disk-shaped upper magnetic member 9a and a lower magnetic member 9b, and a ring-shaped magnetic wire of insulated conductors installed so as to be sandwiched between the upper magnetic member 9a and the lower magnetic member 9b. A coil 9c is provided. These magnetic members 9 a and 9 b and the magnetic coil 9 c are fixed to the drive shaft 4 of the motor 5 and rotate together with the rotor 6. The rotating magnetic coil 9c is supplied with current from the control device 10 via a pair of slip rings (not shown).

  Thus, when a current is passed through the magnetic coil 9c, a magnetic field is generated in the magnetic coil 9c. For example, the test tube holder 7 made of a magnetic material such as SUS430 material has an upper magnetic member 9a and a lower magnetic member. Since the magnetic circuit is formed together with 9b, it is strongly adsorbed by the upper magnetic member 9a and the lower magnetic member 9b (magnetic element 9). That is, when a current is applied to the magnetic coil 9c of the magnetic element 9, the magnetic element 9 (magnetic members 9a and 9b) acts as one magnet and attracts the test tube holder 7 made of a magnetic material. In this embodiment, the outer diameter of the upper magnetic member 9a is set to be larger than that of the lower magnetic member 9b. Therefore, the attracting surfaces of the magnetic members 9a and 9b (magnetic element 9) are As shown in FIG. 2 (3), the test tube holder 7 can be adsorbed so that the test tube 8 is opened at a predetermined angle θ1 (about 8 °) upward with respect to the vertical line.

  By turning on the operation of the magnetic element 9 as described above, the test tube holder 7 is attracted in a state of being tilted by a small angle θ1 that is perpendicular or nearly perpendicular, as shown in FIG. By turning off the operation and releasing the adsorption force, the test tube holder 7 is rotated in the horizontal direction by the centrifugal force acting according to the rotation speed. By turning the operation of the magnetic element 9 on and off in small increments, the test tube holder 7 can be swung as shown in FIG.

  On the other hand, as described later, in another process of the cleaning process (centrifugation process (see FIG. 2B)), the rotor 6 is rotated at a high speed in a state where the operation of the magnetic element 9 is turned off and the attractive force is released. Then, the test tube holder 7 is rotated in the horizontal direction by the centrifugal force, whereby the test tube holder 7 holding the test tube 8 is rotated in the horizontal direction, and the lower portion of the test tube holder 7 together with the rotor 6 is rotated. The sample such as blood cells in the test tube 8 held by the test tube holder 7 is centrifuged until it hits the rotating bowl 11. For example, in a state where the operation of the magnetic element 9 is turned off and the attraction force is released. When the lower end of the test tube holder 7 is brought into contact with the bowl 11 by rotating the motor 5 at 3000 rpm, the test tube holder 7 has an angle formed by the test tube 8 and a vertical line (a line parallel to the rotor central axis 6a). θ2 is about 40 ° Note that, for example, an induction motor is used as the motor 5, and the number of rotations (rotational speed) is controlled by the control device 10.

  The cell washing centrifuge 1 according to the present invention includes a washing liquid distribution element 12 for supplying a washing liquid into the plurality of test tubes 8. The cleaning liquid distribution element 12 is configured on the rotor 6 so as to rotate integrally with the rotor 6, and a cleaning liquid supply path 13 for supplying the cleaning liquid to the cleaning liquid distribution element 12 is provided. A liquid feed pump 14 is coupled to the cleaning liquid supply path 13, and the cleaning liquid is supplied from a cell cleaning liquid container (not shown) by turning on the operating power of the liquid pump 14 by the control device 10. 13 can be supplied to a cleaning liquid supply nozzle 13 a located at the top of the cell washing centrifuge 1. In the cleaning liquid injection process (see FIG. 2 (1)), which will be described later, the cleaning liquid ejected downward from the cleaning liquid supply nozzle 13a enters the central portion of the cleaning liquid distribution element 12 that rotates at a high speed integrally with the rotor 6, and the cleaning liquid distribution element 12 The flow is divided to the outer periphery by the centrifugal force inside, and is branched into the same number (24) of channels as the test tubes 8 held by the test tube holder 7, and vigorously from the inlet formed on the outer periphery of the cleaning liquid distribution element 12. It is injected into each test tube 8.

A blood cell washing process for performing, for example, a blood transfusion test or the like by the cell washing centrifuge 1 configured as described above will be described below with reference to FIG.
(1) Cleaning liquid injection process:
First, in the washing liquid injection step shown in FIG. 2 (1), the same number (24) of the test tube holders 7 holding the 24 test tubes 8 in which an appropriate amount of living cells such as erythrocytes are previously stored are provided in the motor 5 ( Centrifugal force is applied by rotating the rotor 6) so that the maximum rotational speed (rotational speed) reaches 3000 rpm. As described above, the cell washing liquid (for example, physiological saline) is divided into the outer periphery of the washing liquid distribution element 12 by centrifugal force, and the same number (24) of each flow as the test tubes 8 held in the test tube holder 7 is used. The liquid is branched into a path and is injected into each test tube 8 from the outer periphery of the cleaning liquid distribution element 12 with vigorous force. The injected cleaning liquid hits the inner wall of each test tube 8 located outside the cleaning liquid distribution element 12, travels along the wall surface, and floats the living cells at the bottom of the test tube 8 to create a suspended state. When an appropriate amount of cleaning liquid is injected into the test tube 8, the operation of the liquid feed pump 14 is stopped by the control device 10 and the cleaning liquid injection process is completed.
(2) Centrifugal process:
Subsequently, as shown in FIG. 2 (2), conditions for high-speed rotation such as the present embodiment where floating living cells settle on the bottom of the test tube 8 and unnecessary substances such as serum remain in the supernatant. Then, high speed rotation of the rotor 6 is continued at 3000 rpm for 35 seconds to perform centrifugation. Then, the rotation of the motor 5 is stopped after the centrifugation.
(3) Supernatant discharge process:
Next, as shown in FIG. 2 (3), when the control device 10 energizes the magnetic coil 9c of the magnetic element 9 (that is, when the operation of the magnetic element 9 is turned on), the magnetic element 9 is made of a magnetic material. The test tube holder 7 is sucked and held. As described above, since the outer diameter of the upper magnetic member 9a of the magnetic element 9 is set slightly larger than that of the lower magnetic member 9b, the surface of the magnetic element 9 that attracts the test tube holder 7 faces upward. Therefore, the test tube holder 7 and the test tube 8 held by the test tube holder 7 are held in an almost vertical state inclined upward by the same angle θ1 (about 8 °). Is done.

Next, when the motor 5 is rotated at a low speed of about 400 rpm while the magnetic element 9 is energized, the supernatant liquid in the test tube 8 is applied to the inner wall surface of the test tube 8 by centrifugal force due to the rotation of 400 rpm. Ascending and discharging to the outside, living cells such as red blood cells at the bottom of the test tube 8 remain at the bottom.
(4) Oscillation process:
When the supernatant discharge process is completed, in the next swinging process, as shown in FIG. 2 (4), the motor 5 repeatedly rotates and stops in small increments. As a result, the test tube holder 7 is swung in the outer peripheral direction by centrifugal force due to rotation, and is given a swing by colliding with the magnetic element 9 when stopped, and the cell mass that has settled and settled on the bottom of the test tube 8 is removed. It fulfills the function of understanding.

  By taking the above-described process as one washing cycle and repeating this cycle 3 to 4 times, living cells such as red blood cells in the test tube 8 can be washed, and foreign substances such as antibodies can be more completely separated and removed.

  The test tube 8 in which living cells such as red blood cells washed as described above remain is taken out by opening the door 3 of the cell washing centrifuge 1.

  Next, the gist of the present invention will be described with reference to FIGS.

  FIG. 3 is a partial perspective view showing a state in which the door is opened after cell washing in the cell washing centrifuge according to the present invention, and FIG. 4 is a part showing a sealed state between the main chamber and the door cover by the cover packing of the cell washing centrifuge. FIG. 5 is a partial cross-sectional view showing a state in which the main body chamber and the door cover are sealed by a cover packing according to another embodiment.

  As shown in FIG. 3, the door 3 that opens and closes the upper portion of the housing 2 is attached to the upper portion of the housing 2 by a hinge 16 so as to be pivotable up and down, and the rotor 6 housed in the housing 2. The plurality of test tube holders 7 and the cleaning liquid distribution element 12 supported thereby are covered with a main body chamber 17. A door cover 18 that covers the upper opening in the rotor chamber 21 is provided on the inner surface of the door 3, and a cover formed in a ring shape by an elastic body such as rubber on the outer periphery of the door cover 18. The packing 19 is fitted.

  As shown in FIG. 4, the cover packing 19 includes a lip portion 19a that is bent in a C-shape toward the outer side at a substantially right angle and a support portion 19b that supports the lip portion 19a. Is closed, the lip portion 19a of the cover packing 19 is brought into close contact with the sealing surface 17b of the chamber 17 to seal the inside of the rotor chamber 21, thereby reliably preventing liquid leakage from the main body chamber 17.

  Thus, in the present invention, the door 3 is closed and the cover packing 19 is in close contact with the peripheral edge of the upper surface opening of the main body chamber 17 as shown in FIG. A cylindrical space S is formed between the cover 18 and the outer peripheral surface. Specifically, by setting the inner diameter of the lip portion 19 a of the cover packing 19 to be larger than the outer diameter of the door cover 18, a cylindrical shape is formed between the inner peripheral surface of the cover packing 19 and the outer peripheral surface of the door cover 18. A space S is formed.

  As described above, when the cylindrical space S is formed between the inner peripheral surface of the cover packing 19 and the outer peripheral surface of the main body chamber 17, it sinks into the gap between the main body chamber 17 and the door cover 18 and accumulates in a liquid state. Even if the drain liquid is opened as shown in FIG. 5, the inner peripheral surface of the lip portion 19 a of the cover packing 19 provided to form a space S between the door cover 18 and the outer peripheral surface of the door cover 18. Does not flow to the sealing surface of the lip portion 19a and flows along the outer periphery of the door cover 18 along the groove formed by the space S between the cover packing 19 and the door cover 18, and is positioned at a predetermined position in the main body chamber 17. Fall into. For this reason, the drain liquid does not fall on the casing 2a other than the chamber upper portion 17a, the surroundings are not contaminated, and the maintenance of the cell washing centrifuge 1 is simplified.

  When the cover packing 19 as shown in FIG. 4 is used, if the sealing surface of the lip 19a of the cover packing 19 is inclined with respect to the sealing surface of the main body chamber 17, the cover packing 19 and the main body chamber 17 Although the hermeticity is ensured by the line contact, the drain liquid slightly accumulates in the space formed by the gradient, and the drain liquid accumulated in the gradient portion may sag when the door 3 is opened.

  Therefore, as shown in FIG. 6, the cross-sectional shape of the cover packing 19 ′ lip portion 19 a ′ is inverted T-shaped so that the sealing surface of the lip portion 19 a ′ in contact with the main body chamber 17 is parallel to the main body chamber 17. By doing so, the sealing property of the cover packing 19 ′ and the main body chamber 17 is enhanced, and no gradient is formed on the sealing surface of the lip 19 a ′ of the cover packing 19 ′ and the sealing surface of the main body chamber 18. For this reason, most of the drain liquid that has entered the gap between the main body chamber 17 and the door cover 18 and has accumulated in the liquid state is opened between the cover packing 19 ′ and the door cover 18 when the door 3 is opened as shown in FIG. 5. It flows along the outer periphery of the door cover 18 along the groove formed by the space S ′ and falls to a predetermined position in the main body chamber 17.

  Further, as shown in FIG. 6, the thickness t2 of the support portion 19b ′ of the cover packing 19 ′ is made thinner than the thickness t1 of the lip portion 19a ′ (t1> t2), so that the support portion 19b ′ has elasticity. Thus, the cover packing 19 ′ and the main body chamber 17 can be easily sealed.

  Further, as shown in FIG. 6, the support portion 19b ′ for supporting the lip portion 19a ′ of the cover packing 19 ′ is opposite to the door cover 18 side with respect to the center in the width direction of the cover packing mounting portion 19c ′ (left side in FIG. 6) Since the larger space S ′ can be formed between the cover packing 19 ′ and the door cover 18, the amount of drain liquid accumulated between the cover packing 19 ′ and the door cover 18 is increased, and the door 3 is When opened, the flow of the drain liquid flowing along the groove formed by the space S ′ between the cover packing 19 ′ and the door cover 18 has priority, and the flow of the drain liquid to the sealing surface of the lip portion 19a ′ is eliminated. be able to.

It is sectional drawing which shows the whole structure of the cell washing centrifuge which concerns on this invention. It is principal part sectional drawing which shows the operation state of the test tube holder in each washing process process of the cell washing centrifuge which concerns on this invention. It is a fragmentary perspective view which shows the state which opened the door after cell washing in the cell washing centrifuge which concerns on this invention. It is a fragmentary sectional view which shows the sealing state of the main body chamber and door cover by the cover packing of the cell washing centrifuge which concerns on this invention. It is the figure which looked at the A section of FIG. 3 from the arrow B direction. It is a fragmentary sectional view which shows the sealing state of the main body chamber and door cover by the cover packing which concerns on another form of this invention. It is a fragmentary sectional view which shows the sealing state of the main body chamber and door cover by the cover packing of the conventional cell washing centrifuge. It is the fragmentary perspective view which looked at the opened door of the conventional cell washing centrifuge from the inside.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Cell washing centrifuge 2 Case 3 Door 4 Drive shaft 5 Motor 6 Rotor 7 Test tube holder 8 Test tube 9 Magnetic element 9a Upper magnetic body member of magnetic element 9b Lower magnetic body member of magnetic element 9c Magnetic coil of magnetic element 10 Control device 11 Bowl 12 Cleaning liquid distribution element 13 Cleaning liquid supply path 13a Cleaning liquid supply nozzle 14 Liquid feed pump 15 Cell cleaning liquid container 16 Hinge 17 Chamber 18 Door cover 19, 19 'Cover packing 19a, 19a' Cover packing lip portion 19b, 19b ' Cover packing support portion 19c 'Cover packing mounting portion 20 Rotating chamber 21 Rotor chamber S, S' space

Claims (3)

A motor as a driving source; a rotor driven to rotate by the motor; a plurality of test tube holders rotatably mounted on the rotor in a circular row; and a plurality of test tubes held by the test tube holders A cleaning liquid distribution element for supplying a cleaning liquid to the apparatus, a holding means for holding the test tube holder at a vertical or near vertical angle, a main body chamber covering the rotor, the test tube holder and the cleaning liquid distribution element, and the main body In a cell washing centrifuge comprising: a door cover that covers an upper portion of the chamber; and a cover packing that is fitted to an outer periphery of the door cover and seals between the main body chamber and the door cover.
A cell washing centrifuge characterized by forming a cylindrical space between an inner peripheral surface of the cover packing and an outer peripheral surface of the door cover.   2. The cell washing centrifuge according to claim 1, wherein the thickness of the support part of the cover packing is made thinner than the thickness of the lip part to give the support part elasticity. The cell washing centrifuge according to claim 2, wherein a support portion that supports the lip portion of the cover packing is offset toward the door cover side with respect to the center in the width direction of the cover packing mounting portion.

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