CN112844875A - Prevent biological cell centrifuge of test tube stopper spun - Google Patents

Prevent biological cell centrifuge of test tube stopper spun Download PDF

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Publication number
CN112844875A
CN112844875A CN202011559518.0A CN202011559518A CN112844875A CN 112844875 A CN112844875 A CN 112844875A CN 202011559518 A CN202011559518 A CN 202011559518A CN 112844875 A CN112844875 A CN 112844875A
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wall
test tube
centrifuge
fixedly connected
preventing
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CN202011559518.0A
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任晓东
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B7/00—Elements of centrifuges
    • B04B7/02—Casings; Lids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B7/00—Elements of centrifuges
    • B04B7/02—Casings; Lids
    • B04B7/06—Safety devices ; Regulating
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B7/00—Elements of centrifuges
    • B04B7/02—Casings; Lids
    • B04B2007/025—Lids for laboratory centrifuge rotors

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Centrifugal Separators (AREA)

Abstract

The invention relates to the technical field of biological cells, and discloses a biological cell centrifuge for preventing a test tube plug from being sprayed out. This prevent test tube stopper spun biological cell centrifuge through shifting up of dielectric plate for piezo-resistor begins work, covers the lid of centrifuge and follows the gaseous separation out in the test tube after, avoids trying to appear intraductal pressure increase, prevents test tube stopper blowout, avoids causing the impact force to the lid, prevents that centrifuge's lid from receiving the harm, improves centrifuge and gets life, avoids causing economic loss.

Description

Prevent biological cell centrifuge of test tube stopper spun
Technical Field
The invention relates to the technical field of biological cells, in particular to a biological cell centrifuge for preventing a test tube plug from being sprayed.
Background
The centrifuge in biological cell engineering is a machine for separating solid particles from liquid in suspension or separating two kinds of components in emulsion with different densities, but part of biological cell extract can dissolve gas after being treated, and the gas can be separated out from the solution under the centrifugal action of the centrifuge, so that the phenomenon that a test tube plug is sprayed out is caused.
Because the cell sap after partial processing can the solvent gas, current centrifuge in centrifugal process need cover the lid of centrifuge, along with gaseous the appearing in the test tube, can make the pressure increase in the test tube, can cause the test tube stopper blowout, causes the impact force to the lid, can damage centrifuge's lid, reduces centrifuge and gets life, causes economic loss.
Therefore, we have proposed a biocellular centrifuge for preventing the ejection of the test tube stopper to solve the above problems.
Disclosure of Invention
Technical scheme (I)
In order to realize the purposes of avoiding the increase of pressure intensity in the test tube, preventing the test tube plug from being sprayed, avoiding the impact force on the cover body, preventing the cover body of the centrifugal machine from being damaged, prolonging the service life of the centrifugal machine and avoiding the economic loss along with the separation of gas in the test tube after the cover body of the centrifugal machine is covered, the invention provides the following technical scheme: a biological cell centrifuge for preventing a test tube plug from being sprayed out comprises a shell, wherein a protection plate is movably connected to the inner wall of the shell, a pressing block is fixedly connected to the top of the protection plate, a dielectric plate is fixedly connected to the inner wall of the pressing block, a positive plate is fixedly connected to the inner wall of the shell and close to the outer side of the pressing block, a negative plate is fixedly connected to the inner wall of the shell and close to the outer side of the pressing block, and a piezoresistor is fixedly connected to the inner wall of the shell and close to the top of the;
the inner wall of the shell is fixedly connected with a first electromagnet, a groove is formed in the bottom of the inner wall of the shell, which is close to the first electromagnet, a piston is movably connected to the inner wall of the groove, a round hole is formed in the inner wall of the piston, and a connecting rod is fixedly connected to the bottom of the piston;
the outside fixedly connected with fixed axle of briquetting, the inner wall elastic connection of fixed axle has the stopper, the inner wall fixedly connected with electro-magnet two of stopper, the inner wall of briquetting and the outside swing joint who is close to the fixed axle have the movable roll, the inner wall fixedly connected with magnetic path of movable roll, the spacing groove has been seted up to the inner wall of movable roll.
Preferably, the structure of guard plate is the cylinder structure, the material of guard plate is the rubber material, the material of briquetting is the rubber material, and the guard plate has played the effect of protection.
Preferably, the shape and size of the positive plate and the negative plate are the same, the length of the dielectric plate is smaller than that of the positive plate, the negative plate is electrically connected with the piezoresistor, the piezoresistor is electrically connected with the electromagnet, and the positive plate and the negative plate play a role in providing voltage.
Preferably, the connecting rod is fixedly connected with the pressing block, the connecting rod is of a cylindrical structure, and the connecting rod plays a role in connection.
Preferably, the piston is closely attached to the inner wall of the groove, the piston is made of rubber, and the piston plays a sliding role.
Preferably, the inner side of the groove is filled with magnetorheological fluid, the magnetorheological fluid mainly comprises carbonyl iron powder and silicone oil, and the groove plays a role in containing the magnetorheological fluid.
Preferably, the magnetism of the opposite surface of the electromagnet II and the magnetic block is opposite, the electromagnet II is electrically connected with the piezoresistor, and the electromagnet II and the magnetic block play a mutual attraction role.
Preferably, the structure of stopper is the cone structure, spacing groove evenly distributed is at the inner wall of activity roller, the size of stopper suits with the size of spacing groove, and the stopper has played spacing effect.
(II) advantageous effects
Compared with the prior art, the invention provides the biological cell centrifuge for preventing the test tube plug from being sprayed, which has the following beneficial effects:
1. this prevent test tube stopper spun biological cell centrifuge through shifting up of dielectric plate for piezo-resistor begins work, covers the lid of centrifuge and follows the gaseous separation out in the test tube after, avoids trying to appear intraductal pressure increase, prevents test tube stopper blowout, avoids causing the impact force to the lid, prevents that centrifuge's lid from receiving the harm, improves centrifuge and gets life, avoids causing economic loss.
2. This prevent biological cell centrifuge of test tube stopper spun, through the characteristic of piezo-resistor, when the voltage in the circuit is greater than piezo-resistor's maximum operating voltage, the circuit that piezo-resistor connected is in the state of short circuit, and when the voltage in the circuit was less than piezo-resistor's maximum operating voltage, the circuit that piezo-resistor connected was in the state of route, and then the change of control magnetorheological suspensions, realized the cyclic utilization of material, improved the utilization ratio of resource, energy-concerving and environment-protective.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is an enlarged partial view of portion A of FIG. 1;
FIG. 3 is a schematic view of the housing structure of the present invention;
FIG. 4 is an enlarged partial view of the portion B in FIG. 3 according to the present invention.
In the figure: 1. a housing; 2. a protection plate; 3. briquetting; 4. a dielectric plate; 5. a positive plate; 6. a negative plate; 7. a voltage dependent resistor; 8. an electromagnet I; 9. a groove; 10. a connecting rod; 11. a piston; 12. a circular hole; 13. a fixed shaft; 14. a limiting block; 15. an electromagnet II; 16. a limiting groove; 17. a movable roller; 18. a magnetic block.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-4, a biological cell centrifuge for preventing the ejection of a test tube plug comprises a casing 1, a protection plate 2 is movably connected to the inner wall of the casing 1, the protection plate 2 is of a cylindrical structure, the protection plate 2 is made of rubber, a press block 3 is made of rubber, the protection plate 2 plays a role of protection, the top of the protection plate 2 is fixedly connected with the press block 3, the inner wall of the press block 3 is fixedly connected with a dielectric plate 4, the inner wall of the casing 1 and the outer side close to the press block 3 are fixedly connected with a positive plate 5, the positive plate 5 and a negative plate 6 are identical in shape and size, the length of the dielectric plate 4 is smaller than that of the positive plate 5, the negative plate 6 is electrically connected with a piezoresistor 7, the piezoresistor 7 is electrically connected with an electromagnet 8, the positive plate 5 and the negative plate 6 play a role of providing voltage, the inner wall of the casing 1, the inner wall of the shell 1 and the top close to the negative plate 6 are fixedly connected with a piezoresistor 7;
the inner wall of the shell 1 is fixedly connected with an electromagnet I8, the bottom, close to the electromagnet I8, of the inner wall of the shell 1 is provided with a groove 9, magnetorheological fluid is filled inside the groove 9, the magnetorheological fluid mainly comprises carbonyl iron powder and silicone oil, the groove 9 plays a role in containing the magnetorheological fluid, the inner wall of the groove 9 is movably connected with a piston 11, the piston 11 is tightly attached to the inner wall of the groove 9, the piston 11 is made of rubber, the piston 11 plays a sliding role, the inner wall of the piston 11 is provided with a round hole 12, the bottom of the piston 11 is fixedly connected with a connecting rod 10, the connecting rod 10 is fixedly connected with the pressing block 3, the connecting rod 10 is of a cylindrical structure, and the connecting rod 10 plays a connecting role;
briquetting 3's outside fixedly connected with fixed axle 13, the inner wall elastic connection of fixed axle 13 has stopper 14, stopper 14's structure is the cone structure, spacing groove 16 evenly distributed is at the inner wall of activity roller 17, stopper 14's size suits with spacing groove 16's size, stopper 14 has played spacing effect, stopper 14's inner wall fixedly connected with electro-magnet two 15, the magnetism of the opposite face of electro-magnet two 15 and magnetic path 18 is opposite, electro-magnet two 15 and piezo-resistor 7 electric connection, electro-magnet two 15 and magnetic path 18 have played the effect of inter attraction, briquetting 3's inner wall and the outside swing joint who is close to fixed axle 13 have activity roller 17, the inner wall fixedly connected with magnetic path 18 of activity roller 17, spacing groove 16 has been seted up to the inner wall of activity roller 17.
The working principle is as follows: putting a test tube to be centrifuged into a centrifuge, covering a shell 1, centrifuging, separating out bubbles dissolved in a test tube solution under the action of centrifugal force, increasing the pressure in the test tube, ejecting against a test tube plug, pushing a protection plate 2 to move upwards when the test tube plug is ejected, pushing a pressing block 3 to move upwards under the action of a movable roller 17, moving a dielectric plate 4 upwards at the moment, pushing a piston 11 to move upwards by a connecting rod 10, exchanging magnetorheological fluid through a round hole 12, reducing the relative area of a positive plate 5 and a negative plate 6, reducing the voltage in a circuit, and when the voltage in the circuit is reduced to the maximum working voltage of a piezoresistor 7, starting the piezoresistor 7 to work;
at the moment, the electromagnet II 15 electrically connected with the piezoresistor 7 has magnetism, and because the opposite facing magnetism of the electromagnet II 15 and the magnetic block 18 is opposite, the electromagnet II 15 attracts the magnetic block 18, so that the limiting block 14 is combined with the limiting groove 16, and the movable roller 17 does not roll, thereby braking the pressing block 3;
meanwhile, the electromagnet I8 electrically connected with the piezoresistor 7 has magnetism, so that the electromagnet I8 forms a magnetic field, and then the magnetorheological fluid on the inner side of the groove 9 is changed into viscous colloid from a liquid state under the action of the magnetic field, so that the piston 11 is blocked, the pressing block 3 is quickly braked and stopped, and the casing 1 is prevented from being damaged by the ejection of the test tube plug;
the process is as shown in the third drawing, the cover body of the centrifuge is covered and then separated out along with the gas in the test tube, the increase of the pressure in the test tube is avoided, the test tube plug is prevented from being sprayed out, the impact force on the cover body is avoided, the cover body of the centrifuge is prevented from being damaged, the service life of the centrifuge is prolonged, and the economic loss is avoided.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (8)

1. A biological cell centrifuge for preventing the ejection of a test tube plug, comprising a casing (1), characterized in that: the inner wall of the shell (1) is movably connected with a protection plate (2), the top of the protection plate (2) is fixedly connected with a pressing block (3), the inner wall of the pressing block (3) is fixedly connected with a dielectric plate (4), the outer side, close to the pressing block (3), of the inner wall of the shell (1) is fixedly connected with a positive plate (5), the outer side, close to the pressing block (3), of the inner wall of the shell (1) is fixedly connected with a negative plate (6), and the top, close to the negative plate (6), of the inner wall of the shell (1) is fixedly connected with a piezoresistor (7);
the inner wall of the shell (1) is fixedly connected with a first electromagnet (8), a groove (9) is formed in the bottom, close to the first electromagnet (8), of the inner wall of the shell (1), a piston (11) is movably connected to the inner wall of the groove (9), a round hole (12) is formed in the inner wall of the piston (11), and a connecting rod (10) is fixedly connected to the bottom of the piston (11);
the outside fixedly connected with fixed axle (13) of briquetting (3), the inner wall elastic connection of fixed axle (13) has stopper (14), the inner wall fixedly connected with electro-magnet two (15) of stopper (14), the outer side swing joint that the inner wall of briquetting (3) just is close to fixed axle (13) has movable roller (17), the inner wall fixedly connected with magnetic path (18) of movable roller (17), spacing groove (16) have been seted up to the inner wall of movable roller (17).
2. The biocellular centrifuge for preventing the ejection of a plug from a test tube according to claim 1, wherein: the structure of guard plate (2) is the cylinder structure, the material of guard plate (2) is the rubber material, the material of briquetting (3) is the rubber material.
3. The biocellular centrifuge for preventing the ejection of a plug from a test tube according to claim 1, wherein: the shape and the size of the positive plate (5) and the negative plate (6) are the same, the length of the dielectric plate (4) is smaller than that of the positive plate (5), the negative plate (6) is electrically connected with the piezoresistor (7), and the piezoresistor (7) is electrically connected with the electromagnet I (8).
4. The biocellular centrifuge for preventing the ejection of a plug from a test tube according to claim 1, wherein: the connecting rod (10) is fixedly connected with the pressing block (3), and the connecting rod (10) is of a cylindrical structure.
5. The biocellular centrifuge for preventing the ejection of a plug from a test tube according to claim 1, wherein: piston (11) and the inner wall of recess (9) closely laminate, the material of piston (11) is the rubber material.
6. The biocellular centrifuge for preventing the ejection of a plug from a test tube according to claim 1, wherein: magnetorheological fluid is filled inside the groove (9), and the magnetorheological fluid mainly comprises carbonyl iron powder and silicone oil.
7. The biocellular centrifuge for preventing the ejection of a plug from a test tube according to claim 1, wherein: the magnetism of the opposite surface of the second electromagnet (15) and the magnetic block (18) is opposite, and the second electromagnet (15) is electrically connected with the piezoresistor (7).
8. The biocellular centrifuge for preventing the ejection of a plug from a test tube according to claim 1, wherein: the structure of stopper (14) is the cone structure, spacing groove (16) evenly distributed is at the inner wall of activity roller (17), the size of stopper (14) suits with the size of spacing groove (16).
CN202011559518.0A 2020-12-25 2020-12-25 Prevent biological cell centrifuge of test tube stopper spun Pending CN112844875A (en)

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CN202011559518.0A CN112844875A (en) 2020-12-25 2020-12-25 Prevent biological cell centrifuge of test tube stopper spun

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009153387A (en) * 2007-12-25 2009-07-16 Hitachi Koki Co Ltd Cell-washing centrifugal machine
CN111804445A (en) * 2020-07-14 2020-10-23 黄蕾 Automatic locking biological cell test-tube rack in centrifuge operation
CN111974556A (en) * 2020-08-27 2020-11-24 黄星毅 Cell centrifuge fixing mechanism for biological cell research and application thereof
CN112044607A (en) * 2020-08-31 2020-12-08 广州万顺生物科技有限公司 Test tube rack device for automatically locking biological cells by using centrifugal force
CN112076896A (en) * 2020-08-31 2020-12-15 广州逸彬科技有限公司 Biological cell is experimental with equipment that can provide self-protection to cell test tube
CN112076898A (en) * 2020-08-31 2020-12-15 广州万顺生物科技有限公司 Biological cell separation auxiliary device based on magnetorheological body characteristics

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009153387A (en) * 2007-12-25 2009-07-16 Hitachi Koki Co Ltd Cell-washing centrifugal machine
CN111804445A (en) * 2020-07-14 2020-10-23 黄蕾 Automatic locking biological cell test-tube rack in centrifuge operation
CN111974556A (en) * 2020-08-27 2020-11-24 黄星毅 Cell centrifuge fixing mechanism for biological cell research and application thereof
CN112044607A (en) * 2020-08-31 2020-12-08 广州万顺生物科技有限公司 Test tube rack device for automatically locking biological cells by using centrifugal force
CN112076896A (en) * 2020-08-31 2020-12-15 广州逸彬科技有限公司 Biological cell is experimental with equipment that can provide self-protection to cell test tube
CN112076898A (en) * 2020-08-31 2020-12-15 广州万顺生物科技有限公司 Biological cell separation auxiliary device based on magnetorheological body characteristics

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Application publication date: 20210528