JP6912112B2 - Microbial detector - Google Patents

Description

The present invention relates to a microorganism detection device, and is suitable for, for example, a microorganism detection device using dielectrophoresis.

For example, in a beverage maker, the detection of microorganisms such as bacteria and yeast is exclusively performed by a culture method. In this culture method, an agar medium is provided on a petri dish, and microorganisms are coated on the surface of the petri dish and kept at a predetermined temperature to grow until the microorganisms can be detected. Therefore, it is unavoidable to spend a great deal of time and labor on the work of detecting microorganisms, and there is a problem that it is not suitable for immediate monitoring.

Various studies have been conducted on methods for detecting microorganisms using such defect-free dielectrophoresis. For example, in Patent Document 1, a sample liquid containing dielectric fine particles (microorganisms) is sucked from the sample liquid holding portion through a cell with a liquid feed pump, and the dielectric fine particles in the sample liquid are collected by a dielectrophoretic electrode in the cell. Then, it is disclosed that the release liquid is passed through the dielectrophoresis electrode to concentrate and recover the dielectric fine particles collected by the dielectrophoresis electrode.

According to this, the concentrated dielectric fine particles can be easily recovered as a target bacterium. Further, it is described that the dielectric fine particles collected on the dielectrophoretic electrode can be observed in real time with a CCD camera, an optical microscope, or the like, and the metabolic activity state of the dielectric fine particles can be observed in real time. ..

By the way, the flow path element is discarded after being used about once or twice. However, in the above-mentioned conventional technique, it is difficult to attach / detach the flow path element, and there is a request for facilitation of such work. ..

The present invention has been made in view of such circumstances, and an object of the present invention is to provide a microorganism detection device capable of facilitating attachment / detachment work of a flow path element.

The present invention includes a housing, a flow path element holding portion that holds a flow path element provided inside the housing and having a minute flow path so that the flow path element is horizontal, and the housing. A connection portion that electrically connects the flow path element provided inside and held by the flow path element holding portion to a voltage generating portion that generates a voltage, and a connection portion provided inside the housing and said to flow path. An optical microscope, which is arranged above the flow path element held by the element holding portion and observes microorganisms contained in a fluid flowing through the minute flow path of the flow path element, is provided from the front side of the housing. By sliding-displace the flow path element with respect to the flow path element holding portion, the flow path element can be attached to and detached from the flow path element holding portion, and the flow path element is the flow path element of the flow path element. It has a plurality of element-side terminals that are collectively arranged in one place, and the connection portion corresponds to each of the plurality of element-side terminals and is electrically connected to each of the plurality of element-side terminals. The flow path element has a plurality of connection portion side terminals and is arranged on the back side of the flow path element in the mounting direction with respect to the flow path element held by the flow path element holding portion. When the flow path element is mounted, it is engaged with the flow path element, and when the flow path element is disengaged from the flow path element holding portion, the engagement with the flow path element is released. It relates to a microorganism detection device configured to be such.

According to the present invention, the housing, the flow path element holding portion that holds the flow path element provided inside the housing and having a minute flow path so that the flow path element is horizontal, and the housing. A connection portion provided inside the body and held by the flow path element holding portion for electrically connecting the flow path element to a voltage generating portion for generating a voltage, and a connection portion provided inside the housing, said to be provided. An optical microscope which is arranged above the flow path element held by the flow path element holding portion and observes microorganisms contained in a fluid flowing through the minute flow path of the flow path element is provided, and is provided on the front side of the housing. Therefore, by sliding-displace the flow path element with respect to the flow path element holding portion, the flow path element can be attached to and detached from the flow path element holding portion, and the flow path element can be attached to or detached from the flow path element. It has a plurality of element-side terminals that are collectively arranged in one place of the element, and the connection portion corresponds to each of the plurality of element-side terminals and is electrically connected to each of the plurality of element-side terminals. The flow path element holding portion is arranged on the back side in the mounting direction of the flow path element with respect to the flow path element held by the flow path element holding portion having a plurality of connection portion side terminals. When the flow path element is mounted, the flow path element is engaged with the flow path element, and when the flow path element is disengaged from the flow path element holding portion, the engagement with the flow path element is engaged. Since it is configured to be released, the work of attaching and detaching the flow path element can be facilitated.

As in the invention of claim 2, it is preferable that the position of the optical microscope is adjusted in advance in the front-rear direction, the left-right direction, and the up-down direction.

As in the invention of claim 3, wherein provided in the housing, a syringe support for supporting lifting the syringe supplying fluid to the fine channel of the channel elements which are held by the channel element holding portion The syringe can be attached to and detached from the syringe support by moving the syringe from the front side of the housing to the syringe support, and the syringe support can be attached to the flow. The syringe is supported so that the syringe is arranged directly above the flow path element held by the path element holding portion, and the syringe support portion is such that the syringe is supported by the syringe support portion and the syringe is supported by the syringe support portion. The syringe is connected to the flow path element by being lowered while the flow path element is being held by the flow path element holding portion, and the syringe is connected to the flow path element by being raised. Is preferably configured to be released.

According to the present invention, the housing, the flow path element holding portion that holds the flow path element provided inside the housing and having a minute flow path so that the flow path element is horizontal, and the housing. A connection portion provided inside the body and held by the flow path element holding portion for electrically connecting the flow path element to a voltage generating portion for generating a voltage, and a connection portion provided inside the housing, said to be provided. An optical microscope which is arranged above the flow path element held by the flow path element holding portion and observes microorganisms contained in a fluid flowing through the minute flow path of the flow path element is provided, and is provided on the front side of the housing. Therefore, by sliding-displace the flow path element with respect to the flow path element holding portion, the flow path element can be attached to and detached from the flow path element holding portion, and the flow path element can be attached to or detached from the flow path element. It has a plurality of element-side terminals that are collectively arranged in one place of the element, and the connection portion corresponds to each of the plurality of element-side terminals and is electrically connected to each of the plurality of element-side terminals. The flow path element holding portion is arranged on the back side in the mounting direction of the flow path element with respect to the flow path element held by the flow path element holding portion having a plurality of connection portion side terminals. When the flow path element is mounted, the flow path element is engaged with the flow path element, and when the flow path element is disengaged from the flow path element holding portion, the engagement with the flow path element is engaged. Since it is configured to be released, the work of attaching and detaching the flow path element can be facilitated.

It is a perspective view which shows the appearance of the microorganism detection apparatus which concerns on one Embodiment of this invention. It is a perspective view which shows the internal structure of this apparatus. It is a perspective view which shows around the syringe. It is a front view which shows around the syringe. It is a top view which shows the circumference of a substrate. It is a block diagram which shows the control system of this apparatus. It is a flowchart which shows the operation example of this apparatus. It is a front view which shows the state before setting of a syringe. It is a front view which shows the state (1) in the setting of a syringe. It is a front view which shows the state (2) in the setting of a syringe. It is a front view which shows the state after setting of a syringe. It is a perspective view which shows the state before setting of a substrate. It is a perspective view which shows the state after setting of a substrate.

The present invention applies a voltage to a flow path element holding portion that holds a flow path element having a minute flow path and the flow path element that is attached to the flow path element holding portion and held by the flow path element holding portion. The connection portion is provided with a connection portion that is electrically connected to the voltage generating portion to be generated, and the connection portion is engaged with the flow path element when the flow path element is mounted on the flow path element holding portion. The present invention relates to a voltage applying device configured to be disengaged from the flow path element when the flow path element is detached from the flow path element holding portion. Hereinafter, a microorganism detection device including this voltage application device will be described as an example. FIG. 1 is a perspective view showing the appearance of the microorganism detection device 1 according to the embodiment of the present invention, FIG. 2 is a perspective view showing the internal configuration thereof, FIG. 3 is a perspective view showing the circumference of the syringe 3, and FIG. 4 is a perspective view around the syringe 3. FIG. 5 is a front view showing, and FIG. 5 is a plan view showing the periphery of the substrate 2. Reference numerals X in FIGS. 1 and 2 indicate the operation side in front of the device.

As shown in FIGS. 1 and 2, the microorganism detection device (hereinafter referred to as the device) 1 according to the embodiment of the present invention includes a substrate (corresponding to a flow path element) 2, a syringe 3, and an optical microscope. The microscope 4 and the microscope 4 are arranged close to each other in the common housing 5, from the syringe 3 erected on the left side when facing the operation side in the housing 5, and near the bottom in the housing 5. The sample solution is supplied to the substrate 2 which is horizontally arranged so as to be insertable and detachable, and the microorganisms contained in the sample solution are hydrolyzed by applying an AC electric field to the sample solution while the sample solution is passing through the substrate. Therefore, it is configured so that it can be imaged by an optical microscope 4 erected on the right side when facing the operation side in the housing 5.

The housing 5 is provided with a box-shaped main body 51, a semi-transparent opening / closing door 52 provided so as to swing up and down from the operation side (front) of the main body 51 to open and close, and both upper left and right sides of the main body 51. It is provided with a grip portion 53 for carrying around. Inside the main body 51, support portions 24, 33, 41 of each component 2 to 4 are arranged so that the components 2 to 4 are close to each other.

As shown in FIGS. 3 and 4, the syringe 3 has a tubular body (syringe in a narrow sense) 31 having a stepped portion 311 on the distal end side and a first flange portion 312 on the proximal end side, and the cylinder. It has a structure having a gasket 321 slidable inside the body 31 and a plunger 32 connected to the gasket 321 and having a second flange portion 322 on the base end side. Their materials are plastic, glass, rubber and so on.

The support portion 33 of the syringe 3 has a square tubular first member 330a erected on the support portion 24 of the substrate 2. Inside the first member 330a, a second member 330b that can be raised and lowered along the inner wall of the first member 330a is provided. The first member 330a is provided with an operation lever 303 (see FIGS. 8 and 11) for raising and lowering the first member 330a. From the lower side of the front wall of the first member 330a toward the middle, the third member 331 that receives the step portion 311 of the syringe 3, the fourth member 332 that elastically sandwiches the cylinder 31 of the syringe 3, and the syringe 3 A fifth member 333 that receives the first flange portion 312 of the above is fixed. Then, the syringe 3 is vertically oriented and moved in the front-rear direction (the direction orthogonal to the advancing / retreating direction of the plunger 32 with respect to the tubular body 31) so that the syringe 3 can be attached to and detached from the fourth member 332 and the like.

Further, in the second member 330b, a sixth member 334 that supports the hook 300 as a mechanism for detachably gripping the plunger 32 of the syringe 3 is opened from the upper side to the middle of the front wall of the first member 330a. It is fixed via the opening 335. As a result, after the entire syringe 3 is detachably attached from the operation side, the tubular body 31 is fixed so as not to move, and only the plunger 32 can be raised and lowered.

The hook 300 is rotatably provided on both the left and right sides of the main body of the hook 300, and opens and closes the claw portion 300a elastically urged by the spring 300b, respectively. Then, by moving the plunger 32 in the vertical direction (forward direction of the plunger 32 with respect to the tubular body 31), the second flange portion 322 of the plunger 32 comes into contact with the claw portion 300a and automatically grips the plunger 32. It has become.

The support portion 33 of the syringe 3 is provided with a plunger drive portion 302 that drives the plunger 32. The plunger drive unit 302 can push and pull the plunger 32 against the tubular body 31 of the syringe 3 vertically fixed by the fourth member 332 of the support unit 33 or the like by raising and lowering the hook 300, and the plunger 32 can be pushed and pulled. By pressing, the liquid is supplied from the cylinder 31 to the substrate 2, and by pulling the plunger 32, the residual liquid can be collected from the substrate 2 to the cylinder 31.

The plunger drive unit 302 has the hook 300 and drives the plunger 32 by driving the hook 300. In addition to the hook 300, the plunger drive unit 302 includes a motor, the second member 330b, and a ball screw mechanism that converts the rotational motion of the motor into the elevating motion of the second member 330b. The motor is a pulse motor that enables continuous liquid supply with a minute amount by the syringe 3. The motor and the ball screw mechanism are provided inside the first member 330a. By rotating the motor, the second member 330b moves up and down. As the second member 330b moves up and down, the hook 300 fixed to the second member 330b moves up and down via the sixth member 334, and the plunger 32 gripped by the hook 300 moves up and down.

The hook 300 has a contact portion 301a that can penetrate the main body of the hook 300 and come into contact with the second flange portion 322 of the plunger 32, and the second flange portion 322 of the plunger 32 with respect to the contact portion 301a. A load cell 301 arranged on the opposite side is provided. Then, the hook 300 is lowered by the drive of the motor, and the plunger 32 is attached to the hook 300 based on the magnitude of the pressing force sensed when the contact portion 301a abuts on the second flange portion 322 of the plunger 32. It is possible to detect whether or not it is mounted, the pressing start position of the plunger 32, and the pressing / cutting position of the plunger 32.

As shown in FIG. 5, the substrate 2 includes a preparation 21 for passing the sample liquid, and an electrode 22 for dielectrophoresing microorganisms in the sample liquid by applying an AC voltage to the passing sample liquid. ..

The slide 21 is provided with a groove portion (corresponding to a minute flow path) 213 in the long direction on the upper surface of a transparent base material having a rectangular shape in a plan view, and both ends of the transparent cover covering the groove portion 213 in the long direction. A liquid inlet 211 and a liquid outlet 212 are formed on the side. A short tube into which the step portion 311 of the syringe 3 can be inserted is erected at the liquid inlet 211, and an opening hole for connecting the waste liquid tube 214 is formed at the liquid outlet 212. Their materials are plastic, glass, etc. The dimensions and shape of the groove 213 are set according to the maximum flow rate of the passing sample liquid.

The electrode 22 is formed in a comb shape by sputtering a conductive material such as nickel on the bottom of the groove 213. The preparation 21 is used once or twice and then discarded, but the residual liquid of the sample liquid supplied therein can be recovered and analyzed.

The support portion 24 of the substrate 2 is installed on the upper surface thereof, and the holding plate (corresponding to the flow path element holding portion) 231 that elastically presses and holds the preparation 21 and the electrode 22 are shown in FIG. It is provided with an electrode support plate (corresponding to a connection portion) 23 that holds an AC power source (corresponding to a voltage generating portion) 8 so that power can be supplied. The electrode 22 has a plurality of terminals (corresponding to element-side terminals) 221 that are collectively arranged in one place of the substrate 2, and the electrode support plate 23 corresponds to each of the plurality of terminals 221 and has a plurality of terminals 22. It has a plurality of terminals (connection portion side terminals) 232 that are electrically connected to each of the terminals 221 of the above.

The holding plate 231 is made of a flexible material, and in a main body having the same width as the preparation 21 in a plan view but slightly longer, the central rear portion, the left and right front sides, and the intermediate front side thereof are largely cut out. It has a deformed shape. The notch in the central rear portion corresponds to the observation range of the optical microscope 4 in which the electrode 22 is arranged, and the notch on the left and right front sides corresponds to the liquid inlet 211 and the liquid outlet 212. The left and right ends of the holding plate 231 are fixed to the support portion 24 with a gap for inserting the preparation 21. Then, the slide 21 is slidably displaced on the support portion 24 and inserted into the gap or escaped from the gap so that the slide 21 can be attached to and detached from the holding plate 231.

The electrode support plate 23 is fitted into the notch at the central rear portion of the holding plate 231 from the rear so as to be arranged on the back side of the preparation 21 held by the holding plate 231 in the mounting direction. And fixed there. Then, when the preparation 21 is inserted into the gap between the holding plate 231 and the support portion 24, the plurality of terminals 232 of the electrode support plate 23 are elastic to the plurality of terminals 221 of the electrodes 22 arranged at the central rear portion of the preparation 21. The AC power supply 8 can supply power to the electrode 22 by contacting the electrodes.

As shown in FIG. 2, the optical microscope 4 includes a camera (CCD camera) 401 capable of outputting imaging data. The support portion 41 of the optical microscope 4 is erected on the support portion 24 along with the support portion 33 of the syringe 3. Then, by appropriately turning the adjustment dials 402, 403, and 404, the position of the optical microscope 4 can be adjusted together with the camera 401 in three dimensions in the front-back, left-right, and up-down directions. Further, by turning on a light (not shown) provided below the center of the preparation 21 of the substrate 2, a clear image captured by the camera 401 of the optical microscope 4 can be obtained.

FIG. 6 is a block diagram showing a control system of the present device 1. As shown in FIG. 6, the control system of the present device 1 includes a controller 6, a personal computer (hereinafter abbreviated as a personal computer) 7, and an AC power supply 8. Based on the detection signal from the load cell 301, the controller 6 determines whether or not the plunger 32 is attached to the hook 300, whether or not it is the pressing start position of the plunger 32, and whether or not it is the pushing-off position of the plunger 32. In addition to controlling the motor, the power supply from the AC power supply 8 to the electrode 22 is controlled, and the power supply voltage and frequency are adjusted within a predetermined range in the sample liquid passing through the groove 213 of the preparation 21. By dielectrophoresing the microorganism, it is collected by the electrode 22. The optical microscope 4 magnifies an image of microorganisms collected by the electrode 22 and captures the image with the camera 401. The personal computer 7 displays the imaging data output from the camera 401 of the optical microscope 4 as a moving image or a still image (photograph) on the screen.

7 is a flowchart showing an operation example of the present device 1, FIG. 8 is a front view showing a state before setting the syringe 3, FIG. 9 is a front view showing a state (1) during setting of the syringe 3, and FIG. 10 is a syringe. 3 is a front view showing the state (2) during setting, FIG. 11 is a front view showing the state after setting the syringe 3, FIG. 12 is a perspective view showing the state before setting the substrate 2, and FIG. 13 is a perspective view of the substrate 2. It is a perspective view which shows the state after setting. It is assumed that a predetermined amount of sample liquid is sucked and held in the syringe 3 in advance.

As shown in FIG. 7, first, the substrate 2 and the syringe 3 are set in the housing 5 of the apparatus 1 (step S1). Here, the opening / closing door 52 on the operation side (front) of the housing 5 is opened, and the slide 21 of the substrate 2 is horizontally attached to a predetermined position of the support portion 24 at the bottom thereof. Specifically, as shown in FIGS. 12 and 13, the slide 21 is slidably displaced on the support portion 24, and the preparation 21 is inserted into the gap between the holding plate 231 and the support portion 24. At this time, the plurality of terminals 232 of the electrode support plate 23 elastically abut on the plurality of terminals 221 of the electrodes 22 arranged at the central rear portion of the preparation 21, so that the AC power supply 8 can supply power to the electrodes 22. ..

Then, the syringe 3 is attached to the support portion 33 on the left side. Specifically, as shown in FIGS. 8 to 11, the syringe 3 is vertically oriented and moved from the operation side (front) to the back side (rear) so that the syringe 3 is attached to the fourth member 332 or the like. Then, the operation lever 303 is pushed down. At this time, the entire syringe 3 is lowered together with the support portion 33 of the syringe 3, and the step portion 311 at the tip of the syringe 3 is inserted into the liquid inlet 211 of the substrate 2. A waste liquid tube 214 is attached to the liquid outlet 212 of the substrate 2, and the tip thereof is connected to a recovery tank (not shown). It is assumed that the position of the optical microscope 4 is adjusted in three dimensions in advance together with the camera 401.

Then, the motor starts pressing the plunger 32 of the syringe 3 (step S2), and presses the plunger 32 until it is detected by the load cell 301 (step S3). At this time, the hook 300 is lowered, and the second flange portion 322 of the plunger 32 of the syringe 3 comes into contact with the claw portion 300a of the hook 300. Then, the claw portion 300a opens to the left and right against the elastic urging force of the spring 300b, sandwiches the second flange portion 322, and then closes. In this way, the hook 300 grips the plunger 32 of the syringe 3. After the plunger 32 is detected by the load cell 301, the sample liquid is continuously supplied into the substrate 2 in a small amount by continuously pressing the plunger 32 with the motor (step S4).

By appropriately adjusting the voltage and frequency of the AC power supply 8 applied to the electrode 22 of the substrate 2 while checking the image captured by the camera 401 of the optical microscope 4 on the screen of the personal computer 7, a dielectrophoresis phenomenon is caused. The microorganisms in the sample solution are collected (step S5). Then, when the load cell 301 detects the push-off of the plunger 32, the pressing is stopped (step S6) to end the work, but if necessary, the residual liquid in the substrate 2 is removed by rotating the motor in the reverse direction. It is also possible to collect the microorganisms in the syringe 3 and separate the microorganisms. Then, in the reverse procedure of step S1, the substrate 2 and the syringe 3 are taken out from the housing 5, and then the opening / closing door 52 is closed. All of the above operations can be performed by one-touch one-handed operation.

As described above, according to the present apparatus 1, the support portion 24 for holding the substrate 2 having the groove portion 213 forming the minute flow path and the substrate 2 attached to the support portion 24 and held by the support portion 24 are provided. The electrode support plate 23 is provided with an electrode support plate 23 that is electrically connected to the AC power source 8 that generates a voltage, and the electrode support plate 23 is engaged with the substrate 2 when the substrate 2 is mounted on the support portion 24. Since the engagement with the substrate 2 is released when the substrate 2 is detached from the support portion 24, the attachment / detachment work of the substrate 2 can be facilitated.

In the above embodiment, the test liquid is supplied to the substrate 2 from the syringe 3, but the liquid supply to the substrate 2 may be directly connected to the production line. In that case, since the syringe 3 is not required, the device configuration becomes even simpler.

Further, in the above embodiment, the step portion 311 of the syringe 3 is inserted into the short tube of the liquid inlet 211 of the substrate 2, but the step portion 211 of the liquid inlet is made only an opening hole, and the step portion 311 is inserted through the packing-filled connecting portion. The portion 311 may be pressed. In that case, by canceling the short tube, more preparations 21 can be stacked and stored in the packaging box, which is convenient.

Further, in the above embodiment, whether or not the plunger 32 of the syringe 3 is attached to the hook 300 and the pressing start position and pressing stop position of the plunger 32 of the syringe 3 are detected by one load cell 301. , Other types of sensors may be used.

Further, in the above embodiment, the controller 6 controls the electrodes 22 and the syringe 3, and the personal computer 7 only displays the image captured by the camera 401 of the optical microscope 4, but all the controls are integrated by the personal computer 7. It may be done in a targeted manner.

Further, in the above embodiment, the temperature of the sample liquids held in the substrate 2 and the syringe 3 is not controlled, but if necessary, a mechanism for controlling the temperature of the sample liquids may be provided.

Further, in the above embodiment, the microorganism detection device is exemplified, but the fluid supply device and the fluid observation device according to the present invention are not limited to this, and are also applied to a wider field such as a medical field and a semiconductor manufacturing field. Of course you can.

1 Microorganism detection device (including voltage application device)
2 Substrate (corresponds to a flow path element)
21 Preparation 211 Liquid inlet 212 Liquid outlet 213 Groove (corresponds to a minute flow path)
22 Electrode 221 terminal (corresponds to the element side terminal)
23 Electrode support plate (corresponding to the connection part)
231 Retaining plate (corresponds to the flow path element holding portion)
232 terminal (corresponds to the terminal on the connection part side)
24 Support 3 Syringe 300 Hook 301 Load cell 301a Abutment 302 Syringe drive 303 Operating lever 31 Cylinder 311 Step 312 First flange 32 Plunger 321 Gasket 322 Second flange 33 Support 332 Fourth member 4 Optical microscope 401 Camera 41 Support 5 Housing 6 Controller 7 Personal computer 8 AC power supply (corresponds to the voltage generator)

Patent Gazette of Patent No. 4548742

Claims (3)

With the housing
A flow path element holding portion that is provided inside the housing and holds a flow path element having a minute flow path so that the flow path element is horizontal.
A connection portion that is provided inside the housing and is held by the flow path element holding portion to electrically connect the flow path element to a voltage generating portion that generates a voltage.
An optical microscope provided inside the housing and arranged above the flow path element held by the flow path element holding portion to observe microorganisms contained in a fluid flowing through the minute flow path of the flow path element. And with
By sliding-displace the flow path element with respect to the flow path element holding portion from the front side of the housing, the flow path element can be attached to and detached from the flow path element holding portion.
The flow path element has a plurality of element-side terminals that are aggregated and arranged at one location of the flow path element.
The connection portion has a plurality of connection portion side terminals corresponding to each of the plurality of element side terminals and electrically connected to each of the plurality of element side terminals, and is held by the flow path element holding portion. It is arranged on the back side of the flow path element in the mounting direction of the flow path element, and when the flow path element is mounted on the flow path element holding portion, it is engaged with the flow path element. A microorganism detection device configured to be disengaged from the flow path element when the flow path element is detached from the flow path element holding portion. The microorganism detection device according to claim 1, wherein the optical microscope is positioned in advance in the front-rear direction, the left-right direction, and the up-down direction. Wherein provided in the housing, further comprising a syringe support portion for supporting lifting the syringe supplying fluid to the fine channel of the channel elements which are held by the channel element holding portion,
By moving the syringe to the syringe support from the front side of the housing, the syringe can be attached to and detached from the syringe support.
The syringe support portion supports the syringe so that the syringe is arranged directly above the flow path element held by the flow path element holding portion.
The syringe support portion is lowered while the syringe is supported by the syringe support portion and the flow path element is held by the flow path element holding portion, whereby the syringe is moved to the flow path element. The microorganism detection device according to claim 1 or 2, wherein the connection between the syringe and the flow path element is released by being connected to and raised from the syringe.

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