CN106190810A - A kind of membrane filtration sterility test device - Google Patents
A kind of membrane filtration sterility test device Download PDFInfo
- Publication number
- CN106190810A CN106190810A CN201610765160.4A CN201610765160A CN106190810A CN 106190810 A CN106190810 A CN 106190810A CN 201610765160 A CN201610765160 A CN 201610765160A CN 106190810 A CN106190810 A CN 106190810A
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- CN
- China
- Prior art keywords
- filter cup
- break
- test device
- membrane filtration
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/02—Filters
Abstract
The invention discloses a kind of membrane filtration sterility test device, including the fixing support of sample bottle, filter cup at least one, sample liquid pipe, drive sample liquid in sample liquid pipe to be delivered to the peristaltic pump of filter cup from sample bottle, as improvement, described each filter cup is provided with detection filter cup liquid level measurer, and described sample liquid pipe is arranged with and filter cup separating tube one to one.The present invention is compared with prior art, Yang Pingdaoping mechanism, break-make structure, perception filter cup liquid level are set or/and the measuring device of pressure, perception liquid level is gathered or/and the information of measuring device of pressure by control system, drive further control Dao Ping mechanism, break-make structure, peristaltic pump carry out corresponding action, reached the purpose of automatic sterilized sampling.Whole process of the present invention sampling need not manual intervention, and automatization, intellectuality are fully achieved.
Description
Technical field
The present invention relates to a kind of membrane filtration sterility test device.
Background technology
In liquid, content of microorganisms inspection generally uses membrane-filter procedure.Existing device discharge opeing is usually direct discharge opeing,
Pumping equipment simple in construction, operation all relies on artificial, needs substantial amounts of manual operation, and operation repeatability is low, and waste resource is also
The service efficiency of lowering apparatus.
Summary of the invention
The present invention solves the problem that prior art in use exists, it is provided that a kind of simple in construction, it is the most fixed to realize
Measure the membrane filtration sterility test device of sample.
The present invention solves the technical scheme of existing issue: a kind of membrane filtration sterility test device, including fixing sample bottle
Support, filter cup at least one, sample liquid pipe, drive sample liquid in sample liquid pipe to be delivered to the peristaltic pump of filter cup from sample bottle, as
Improving, described each filter cup is provided with detection filter cup liquid level measurer, and described sample liquid pipe is arranged with filter cup one by one
Corresponding separating tube；Also include the Yang Pingdaoping mechanism being arranged on sample holder；Control the break-make structure of sample liquid pipe break-make；Institute
The separating tube stated sequentially passes through break-make structure, peristaltic pump, and described break-make structure controls the break-make of separating tube one by one,
Gather the information of corresponding filter cup liquid level measurer and bottle turns in the Dao Ping mechanism of control accordingly, break-make structure closes corresponding point
Liquid pipe or peristaltic pump action, or bottle turns in Dao Ping mechanism, break-make structure closes corresponding separating tube, the combination in any of peristaltic pump action
Control system.
As improving further, described each filter cup is additionally provided with pressometer and controls the valve of pressure, described
Control system gather corresponding pressometer pressure information, and control valve and open or close accordingly.
As improving further, described Dao Ping mechanism includes the fixed mount of sample bottle, drives the driver that fixed mount rotates.
As improving further, described fixed mount includes that the underframe of scalable relative distance is with added.
As improving further, described break-make structure includes the jaws clamping corresponding separating tube and drives corresponding jaws to press from both sides
Jaws driver that is tight or that unclamp.
As improving further, in described corresponding jaws, be provided with the passage of location separating tube, passage be provided with hold out against with
Block the push rod of separating tube.
As improving further, also include a liquid level being connected Non-follow control filter cup with control system or/and pressure
Operation button or touch screen.
As improving further, described liquid level measurer is liquid level sensor or weight sensor.
As improving further, described valve is electromagnetic valve；Above-mentioned support is provided with the detection of detection sample bottle empty bottle
Device and alarm.
The present invention compared with prior art, arrange Yang Pingdaoping mechanism, break-make structure, perception filter cup liquid level or/and
The measuring device of pressure, by control system collection perception liquid level or/and the information of the measuring device of pressure, drives further and controls to fall
Bottle mechanism, break-make structure, peristaltic pump carry out corresponding action, reached the purpose of automatic sterilized sampling.The present invention is omnidistance
Sampling need not manual intervention, and automatization, intellectuality are fully achieved.
Accompanying drawing explanation
Fig. 1 is the structural representation of the present invention.
Fig. 2 is the structural representation of pipeline break-make structure of the present invention.
Fig. 3 is the structural representation of filter cup of the present invention.
Fig. 4 is the schematic diagram of Dao Ping mechanism of the present invention.
Detailed description of the invention
Seeing Fig. 1-4, the implementation case includes: the fixing support 1 of sample bottle 4, filter cup 2 at least one, sample liquid pipe 3, drive
In dynamic sample liquid pipe 3, sample liquid is delivered to the peristaltic pump 5 of filter cup 2, the housing 8 of fixing each parts from sample bottle 4, and each filter cup 2 sets
Having detection filter cup 2 liquid level or/and the measuring device 21 of pressure, sample liquid pipe 3 is arranged with and filter cup separating tube 31 one to one.
Peristaltic pump 5 and support 1 are arranged on housing 8.
Also include: be arranged at the Dao Ping mechanism 6 of sample holder 1 loading bottle 4；
Control the break-make structure 7 of sample liquid pipe 3 break-make；Separating tube 31 sequentially passes through break-make structure 7, peristaltic pump 5, break-make structure 7 one
One break-make controlling separating tube 3,
Gather corresponding filter cup 2 liquid level measurer 21 information and accordingly control bottle turns in Dao Ping mechanism 6, break-make structure 7 is closed
Corresponding separating tube 31 or peristaltic pump 5 action, or bottle turns in Dao Ping mechanism 6, break-make structure 7 closes corresponding separating tube 31, wriggling
The control system of the combination in any of pump 5 action.
Each filter cup 2 is additionally provided with pressometer 21 and controls the valve 22 of pressure, and control system gathers corresponding pressure
Force measurement device pressure information, and control valve 22 and open or close accordingly.Valve 22 is for can pass through solenoid-driven, in order to
Control system controls, and the measuring device 21 of pressure and valve 22 can excellent be arranged on the vent line of filter cup 2.
The control system of valve 22 is separately provided, it is possible to merge the control system using device, or control system is permissible
Independent corresponding components such as Dao Ping mechanism 6, break-make structure 7, valve 22 or the control unit of peristaltic pump 5 is controlled including some.This reality
Execute in case by merge use device control system carry out as a example by.
Dao Ping mechanism 6 includes the fixed mount 61 of sample bottle 4, drives the driver 62 that fixed mount 61 rotates, and driver 62 can be adopted
Use motor.Fixed mount 61 includes the underframe 61a and added 61b of scalable relative distance.In the implementation case underframe 61a with
Added 61b, can be by the slide block adjustable range being arranged on tooth bar 61c, to adapt to corresponding sample bottle 4.Can certainly pass through
Screw rod, nut or slide rail, the distance of slide block regulation underframe 61a and added 61b.
Break-make structure 7 includes the jaws 71 clamping corresponding separating tube and drives corresponding jaws 71 to clamp or the jaws unclamped is driven
Dynamic device, this driver can preferentially use electromagnetic valve, hydraulic pressure or atmospheric pressure pole.Jaws 71 is provided with the jaws 71 adapted with separating tube and sets
There is the groove adapted with separating tube.
Being provided with the passage 72 of location separating tube in corresponding jaws 71, passage 72 is provided with the push rod holding out against to block separating tube
73, push rod 73 can be directly connected to jaws driver, and groove may be disposed on push rod 73.
For the ease of controlling, also include a liquid level being connected Non-follow control filter cup 2 with control system or/and the behaviour of pressure
Make button or touch screen, it is preferred to use touch screen.
Liquid level measurer is liquid level sensor or weight sensor.Support 1 be provided with detection sample bottle 4 empty bottle detector and
Alarm.
Apparatus of the present invention use time, the bottleneck of Dao Ping mechanism 6 loading bottle 4 upwards, break-make structure 7 normally opened, as initial shape
State.When device starts, control system first starts peristaltic pump 5, and then drives Dao Ping mechanism 6 to turn over transfer bottle 4 bottleneck downwards, makes filtration
The valve of cup 2 is in aeration status, and under the effect of peristaltic pump 5, sample liquid starts conveying through sample liquid pipe 3, in moistening filter cup 2
Filter membrane, when control system is by liquid level measurer, monitor after the liquid of filter cup 2 reaches moistening preset value, first drive
Transfer bottle 4 bottleneck turns over upwards in Dao Ping mechanism 6, is then shut off valve 22, discharges the liquid in filter cup 2, when, after emptying rate of liquid, controlling
System processed will open valve 22.Start filtered sample.
During filtered sample, control system first starts peristaltic pump 5, and then drives Dao Ping mechanism 6 to turn over transfer bottle 4 bottleneck downwards,
The valve making filter cup 2 is in aeration status, and under the effect of peristaltic pump 5, sample liquid starts conveying sampling through sample liquid pipe 3, from
Corresponding separating tube 31 enters to corresponding filter cup 2.The liquid level sensor of corresponding filter cup 2 is or/and weight sensor perception
Sample liquid actual liquid level or weight also pass to control system, the liquid level that control system monitors corresponding sample liquid liquid level or weight calculates
Reach preset value, then control corresponding valve 22 and close, increase the pressure in this filter cup 2, make into the sample liquid in filter cup 2
Filtering, if valve 22 hypertonia, can control system by the pressure in pressure monitor sensor filter cup 2 after closing
System can close corresponding separating tube by break-make structure 7, and peristaltic pump 5 is slowed or stopped.
If in sample bottle 4 after emptying rate of liquid, after detector detects the emptying of sample bottle, alarm changes bottle, and control system will
Order about break-make structure 7 and close separating tube.If do not changed bottle, in filter cup 2 after emptying rate of liquid, control system will open valve 22, so
Rear arresting stop runs.
After filtration completes, can be rinsed, after starter, sample bottle 4 is inverted by control system automatic Dao Ping mechanism 6；Logical
Breaking, structure 7 is normally opened not to work；The valve 22 of the opened filter bowl of control system 2, filter cup 2 is in aeration status；When in filter cup 2
Liquid reach a certain amount of after, control system controls break-make structure 7 by this separating tube 31 of closedowns, until in three filter cups 2
Liquid measure all reaches same level, is just put by sample bottle 4, and control system closes the valve 22 of filter cup 2, filters, in filter cup 2
After emptying rate of liquid, the valve 22 of opened control system filter bowl 2, repeats said process the most again.
If in sample bottle 4 after emptying rate of liquid, after detector detects the emptying of sample bottle, alarm changes bottle, and control system will
Order about break-make structure 7 and close separating tube.If do not changed bottle, device stops and recording current state.
When device carries out fill culture medium, can manually close the leakage fluid dram of filter cup 2, control system controls break-make structure 7
To opening a separating tube 31, other Close Alls；After starter, control system controls Dao Ping mechanism 6 and is inverted by sample bottle 4,
The valve 22 of the filter cup 2 being connected with the separating tube 31 opened accordingly will be opened by control system, and this filter cup 2 is in ventilation shape
State；After being filled filter cup 2 liquid level arrival certain altitude, sample bottle is just put by Dao Ping mechanism 6, and after the several seconds, device is shut down, break-make
Structure 7 is changed and is opened separating tube 31, closes and closes a dozen separating tubes 31 opened, and device alarm changes culture medium.(such as it is set to
Same culture medium, instrument may be configured as multitube fill simultaneously, it is also possible to fill the most one by one automatically).
Claims (10)
1. a membrane filtration sterility test device, including the fixing support of sample bottle, filter cup at least one, sample liquid pipe, driving
In sample liquid pipe, sample liquid is delivered to the peristaltic pump of filter cup from sample bottle, it is characterised in that: described each filter cup is provided with and detected
Filter bowl liquid level measurer, described sample liquid pipe is arranged with and filter cup separating tube one to one；Also include
The Yang Pingdaoping mechanism being arranged on sample holder；
Control the break-make structure of sample liquid pipe break-make；Described separating tube sequentially passes through break-make structure, peristaltic pump, described break-make knot
Structure controls the break-make of separating tube one by one,
Gather the information of corresponding filter cup liquid level measurer and bottle turns in the Dao Ping mechanism of control accordingly, break-make structure closes corresponding point
Liquid pipe or peristaltic pump action, or bottle turns in Dao Ping mechanism, break-make structure closes corresponding separating tube, the combination in any action of peristaltic pump
Control system.
2. membrane filtration sterility test device as claimed in claim 1, it is characterised in that: described each filter cup is additionally provided with
Pressometer and the valve of control pressure, described control system gathers corresponding pressometer pressure information, and controls
Valve opens or closes accordingly.
3. membrane filtration sterility test device as claimed in claim 1, it is characterised in that: described Dao Ping mechanism includes sample bottle
Fixed mount, drive fixed mount rotate driver.
4. membrane filtration sterility test device as claimed in claim 3, it is characterised in that: described fixed mount includes scalable
The underframe of relative distance is with added.
5. membrane filtration sterility test device as claimed in claim 1, it is characterised in that: described break-make structure includes clamping
The jaws of corresponding separating tube and drive the clamping of corresponding jaws or the jaws driver unclamped.
6. membrane filtration sterility test device as claimed in claim 5, it is characterised in that: it is fixed to be provided with in described corresponding jaws
The passage of position separating tube, passage is provided with the push rod holding out against to block separating tube.
7. membrane filtration sterility test device as claimed in claim 1 or 2, it is characterised in that: also include one and control system
Connect the liquid level of Non-follow control filter cup or/and the operation button of pressure or touch screen.
8. membrane filtration sterility test device as claimed in claim 7, it is characterised in that: described liquid level measurer is liquid level
Sensor is or/and weight sensor.
9. membrane filtration sterility test device as claimed in claim 2, it is characterised in that: described valve is electromagnetic valve.
10. membrane filtration sterility test device as claimed in claim 1, it is characterised in that: described support is provided with detection
The detector of sample bottle empty bottle and alarm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610765160.4A CN106190810A (en) | 2016-08-30 | 2016-08-30 | A kind of membrane filtration sterility test device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610765160.4A CN106190810A (en) | 2016-08-30 | 2016-08-30 | A kind of membrane filtration sterility test device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106190810A true CN106190810A (en) | 2016-12-07 |
Family
ID=58089770
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610765160.4A Pending CN106190810A (en) | 2016-08-30 | 2016-08-30 | A kind of membrane filtration sterility test device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106190810A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018153341A1 (en) * | 2017-02-23 | 2018-08-30 | 郑州安图生物工程股份有限公司 | Sample adding system for automatically adding sputum digestion liquid |
| CN109061043A (en) * | 2018-09-29 | 2018-12-21 | 杭州启绿科技有限公司 | A kind of permanganate index analyzer and permanganate index analysis method |
| CN109628273A (en) * | 2018-12-28 | 2019-04-16 | 杭州吉沃科技有限公司 | A kind of intelligence sterility test device and its control system |
| CN114634863A (en) * | 2022-03-30 | 2022-06-17 | 上海格氏流体设备科技有限公司 | Tangential flow filter based on cell culture |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018153341A1 (en) * | 2017-02-23 | 2018-08-30 | 郑州安图生物工程股份有限公司 | Sample adding system for automatically adding sputum digestion liquid |
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| CN109628273A (en) * | 2018-12-28 | 2019-04-16 | 杭州吉沃科技有限公司 | A kind of intelligence sterility test device and its control system |
| CN114634863A (en) * | 2022-03-30 | 2022-06-17 | 上海格氏流体设备科技有限公司 | Tangential flow filter based on cell culture |
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161207 |
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| RJ01 | Rejection of invention patent application after publication |