CN114634863A - Tangential flow filter based on cell culture - Google Patents
Tangential flow filter based on cell culture Download PDFInfo
- Publication number
- CN114634863A CN114634863A CN202210332265.6A CN202210332265A CN114634863A CN 114634863 A CN114634863 A CN 114634863A CN 202210332265 A CN202210332265 A CN 202210332265A CN 114634863 A CN114634863 A CN 114634863A
- Authority
- CN
- China
- Prior art keywords
- block
- spring
- bottle
- assembly
- shell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000004113 cell culture Methods 0.000 title claims abstract description 32
- 238000001914 filtration Methods 0.000 claims abstract description 63
- 230000005540 biological transmission Effects 0.000 claims abstract description 52
- 239000007788 liquid Substances 0.000 claims description 23
- 238000007789 sealing Methods 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 4
- 238000000034 method Methods 0.000 abstract description 11
- 230000008569 process Effects 0.000 abstract description 8
- 230000002349 favourable effect Effects 0.000 abstract 2
- 230000009471 action Effects 0.000 description 22
- 238000004140 cleaning Methods 0.000 description 14
- 230000000694 effects Effects 0.000 description 10
- 239000011159 matrix material Substances 0.000 description 8
- 238000010586 diagram Methods 0.000 description 5
- 230000033001 locomotion Effects 0.000 description 4
- 238000010367 cloning Methods 0.000 description 3
- 230000009466 transformation Effects 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 108020005196 Mitochondrial DNA Proteins 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000010370 cell cloning Methods 0.000 description 1
- 238000012832 cell culture technique Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000012411 cloning technique Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
- B01D29/96—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor in which the filtering elements are moved between filtering operations; Particular measures for removing or replacing the filtering elements; Transport systems for filters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to a tangential flow filter based on cell culture, which comprises a bottom plate and a side plate, wherein the side plate is vertically arranged on one side of the bottom plate, the upper surface of the bottom plate is provided with an accommodating groove, a clamping assembly is arranged in the accommodating groove in a sliding manner, one side of the clamping assembly is provided with a transmission assembly in a swinging manner, the corner of the lower surface of the bottom plate is fixedly provided with a supporting leg, one side of the side plate is provided with a first guide groove, and a first filter shell and a second filter shell are arranged in the first guide groove in a sliding manner. When the bottle that will be equipped with the cell base and the bottle of dress filtration back cell base put into the storage tank, second spring and fifth spring pass through the grip block simultaneously and carry out the centre gripping to the bottle that is equipped with the cell base and the bottle of dress filtration back cell base fixed, and in this process, the initial condition of centre gripping subassembly, third spring make the grip block slope, are favorable to placing of bottle, and the grip block holding surface is the arc surface, are favorable to the centre gripping and the fixing to the bottle.
Description
Technical Field
The invention relates to the technical field of cell culture, in particular to a tangential flow filter based on cell culture.
Background
Cell culture refers to a method of simulating the in vivo environment in vitro, allowing it to survive, grow, reproduce and maintain the main structure and function. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Cell culture is an essential process for both the whole bioengineering technique and one of the biological cloning techniques, and is itself a large-scale cloning of cells. The cell culture technology can be used for culturing a single cell into simple single cells or cells with few differentiation in a large scale, which is an indispensable link of the cloning technology, and the cell culture is the cloning of the cell. Cell culture techniques are important and commonly used in cell biology research methods, and a large number of cells can be obtained through cell culture, and signal transduction, anabolism, growth and proliferation of cells and the like of the cells can be researched.
For cells with excessively high mitochondrial gene proportion, cell grouping can be interfered, a threshold value is usually designed according to actual conditions to filter the cells in the analysis process in order to avoid the influence of the cells, and the existing cell filtering equipment has independent operation of each part, is complicated and complex, and greatly reduces the use efficiency.
In order to solve the above problems, the present invention proposes a tangential flow filter based on cell culture.
Disclosure of Invention
(1) Technical problem to be solved
The present invention aims to overcome the problems of the prior art and to provide a tangential flow filter based on cell culture, which is suitable for practical use, so as to solve the technical problems.
(2) Technical scheme
In order to realize the purpose of the invention, the technical scheme adopted by the invention is as follows: a tangential flow filter based on cell culture comprises a bottom plate and a side plate, wherein the side plate is vertically arranged on one side of the bottom plate, the upper surface of the bottom plate is provided with an accommodating groove, a clamping assembly is arranged in the accommodating groove in a sliding manner, one side of the clamping assembly is provided with a transmission assembly in a swinging manner, supporting legs are fixedly arranged at the lower surface edge of the bottom plate, one side of the side plate is provided with a first guide groove, a first filter shell and a second filter shell are arranged in the first guide groove in a sliding manner, one side of the first filter shell is provided with a second filter shell, an auxiliary assembly is fixedly arranged on the upper surface of the first filter shell, assembly assemblies are fixedly arranged on the two side surfaces of the first filter shell, a telescopic assembly is arranged below the second filter shell, and a first spring is fixedly arranged on the lower surfaces of the first filter shell and the second filter shell, the upper surface of the second filtering shell is fixedly provided with a pipeline, one end of the pipeline is fixedly arranged on the upper surface of the second filtering shell, and the other end of the pipeline is fixedly arranged on the upper surface of the first shell.
Preferentially, the centre gripping subassembly includes the second guide way, and this second guide way is seted up in the both sides of storage tank, it is provided with the slide bar to slide in the second guide way, and the one end fixed surface of this slide bar is provided with the second spring, and the other end swing is provided with the grip block, and the lower part fixed surface of this grip block is provided with the third spring, and the one end of this third spring is fixed to be set up on the side surface of grip block, and the other end is fixed to be set up on the inner wall of assembly groove, the transmission groove has been seted up to the one end of slide bar, is provided with drive assembly in this transmission groove, the last fixed surface of second filter housing is provided with the pipeline, and the one end of this pipeline is fixed to be set up in the upper surface of second filter housing, and the other end is fixed to be set up in first filter housing upper surface.
Preferentially, the transmission assembly comprises a supporting plate and a supporting column, a supporting column is arranged on one side of the supporting plate, the supporting plate and the supporting column are fixedly arranged on the upper surface of the bottom plate, a first transmission rod is arranged on the supporting plate in a rotating mode, a second transmission rod is arranged at one end of the supporting column in a rotating mode, the end portion of the first transmission rod is arranged in a transmission groove, and the other end of the first transmission rod is arranged in the middle of the second transmission rod.
Preferably, the auxiliary assembly comprises a guide block, the guide block is arranged on the upper surface of the first filtering shell, a fourth spring is fixedly arranged on the lower bottom surface of the guide block, a sliding block is fixedly arranged on the fourth spring, and the sliding block is arranged on the guide block in a sliding manner.
Preferentially, the fixed air extractor that is provided with of upper surface of first filter housing, be provided with the filter screen below the first filter housing, be provided with the weeping casing below this filter screen, filter screen edge adopts rubber materials.
Preferentially, the assembly component includes first fixed block and second fixed block, is provided with the second fixed block below this first fixed block, the fixed side that sets up at first filtration casing of first fixed block, the fixed setting of second fixed block is on the weeping casing, it is provided with the dog to rotate on the first fixed block, is provided with the torsional spring on this dog, the inboard of dog is provided with first pendulum piece, and this first pendulum piece rotates and sets up on first fixed block, be provided with the pivot on the second fixed block, is provided with the second pendulum piece in this pivot, and this second pendulum piece upper end sets up in one side of first pendulum piece.
Preferably, the telescopic assembly comprises a telescopic cylinder, the telescopic cylinder is arranged below the second filtering shell in a sliding mode, a fifth spring is arranged on the outer side of the telescopic cylinder, and a sealing ring is arranged at the upper end of the telescopic cylinder.
(3) Has the advantages that:
A. when the bottle with the cell matrix and the bottle with the filtered cell matrix are placed in the accommodating groove, the bottle can apply force to the clamping plate to enable the clamping plate and the sliding rod fixedly arranged on the clamping plate to slide along the second guide groove, meanwhile, the second spring and the fifth spring clamp and fix the bottle with the cell matrix and the bottle with the filtered cell matrix through the clamping plate, in the process, the clamping assembly is in an initial state, the third spring enables the clamping plate to incline to facilitate placement of the bottle, and the clamping surface of the clamping plate is an arc surface to facilitate clamping and fixing of the bottle;
B. when the bottle with the cell matrix and the bottle with the filtered cell matrix are placed in the accommodating groove, the bottle can apply force to the clamping plate to enable the clamping plate and the sliding rod fixedly arranged on the clamping plate to slide along the second guide groove, the rear sliding rod enables the first transmission rod to swing through the transmission groove in the end part of the rear sliding rod, the first transmission rod drives the second transmission rod to swing while swinging, and finally the second transmission rod swings to transmit the force to the auxiliary assembly;
C. the second transmission rod swings to transmit force to the sliding block in the auxiliary assembly, so that the sliding block slides downwards along the guide block, meanwhile, the fourth spring is extruded, the force is transmitted to the first filtering shell, the first filtering shell moves downwards along the first guide groove until the leakage shell contacts the bottle mouth of the bottle containing the filtered cell matrix, and in the action structure, the fourth spring can reduce impact generated during transmission of the transmission assembly, so that the bottle mouth of the bottle containing the filtered cell matrix is protected;
D. when the filter screen needs to be cleaned, the first swing block is manually swung towards the axial direction of the first filter shell, then overcomes the torsion of the torsion spring to manually rotate the stop block towards the axis direction of the first filter shell, then the first swing block is put down, meanwhile, when the second swing block fixedly connected with the first swing block swings, the second swing block moves downwards to separate the liquid leaking shell and the filter screen arranged on the liquid leaking shell from the lower surface of the first filter shell, at the moment, the filter screen is taken out from the side surface of the auxiliary assembly to be cleaned, and after the cleaning is finished, the filter screen is placed on the upper surface of the leakage shell along one side of the auxiliary assembly again, after the placement is finished, the first swing block is shifted upwards to enable the leakage shell to move upwards to press the filter screen, the assembly is finished, the arrangement of the structure enables the filter screen to be detached for cleaning, so that not only is the filtering effect of the filter screen improved, but also the structure is simple and acts reliably and rapidly.
E. First filter housing moves down along first guide way, and when the weeping casing contacted the bottle bottleneck of dress filtration back cell base, filter the casing with first filter housing fixed connection's second and move down, the telescopic tube extends to the bottle bottom, is provided with the fifth spring in the outside of telescopic tube, and when the telescopic tube touched the bottle end, can take place to warp in order to realize the effect at the bottom of the protection bottle.
Drawings
FIG. 1 is a schematic perspective view of a tangential flow filter for cell culture based on the present invention;
FIG. 2 is a schematic diagram of the structure of a clamping assembly of a tangential flow filter based on cell culture according to the present invention;
FIG. 3 is a schematic diagram of the construction of an assembly of a cell culture based tangential flow filter according to the invention;
FIG. 4 is a schematic diagram of the structure of a filter screen of a tangential flow filter based on cell culture according to the present invention;
FIG. 5 is a schematic diagram of the construction of a cell culture based tangential flow filter expansion assembly according to the present invention;
FIG. 6 is a schematic view showing the structure of a first guide groove of a tangential flow filter for cell culture based on the present invention;
FIG. 7 is a schematic diagram of the structure of an auxiliary assembly of a tangential flow filter for cell culture based on the present invention.
The reference numbers are as follows:
1-bottom plate, 11-receiving groove, 12-supporting leg, 2-side plate, 21-first guide groove, 211-first spring, 3-clamping component, 31-second guide groove, 32-sliding bar, 33-second spring, 34-clamping plate, 35-third spring, 36-transmission groove, 4-transmission component, 41-supporting plate, 42-supporting column, 43-first transmission bar, 44-second transmission bar, 5-first filtering shell, 51-air extractor, 52-filtering screen, 53-liquid leakage shell, 6-second filtering shell, 61-pipeline, 7-auxiliary component, 71-guide block, 72-fourth spring, 73-sliding block, 8-assembly component, 81-first fixing block, 82-a second fixed block, 83-a stop block, 84-a torsion spring, 85-a first swing block, 86-a rotating shaft, 87-a second swing block, 9-a telescopic component, 91-a telescopic cylinder, 92-a fifth spring and 93-a sealing ring.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
The invention will be further illustrated with reference to the following figures 1 to 7 and examples:
in this embodiment, as shown in fig. 1-7, a tangential flow filter based on cell culture comprises a bottom plate 1 and a side plate 2, wherein the side plate 2 is vertically disposed on one side of the bottom plate 1, an accommodating groove 11 is disposed on the upper surface of the bottom plate 1, a clamping component 3 is slidably disposed in the accommodating groove 11, a transmission component 4 is swingably disposed on one side of the clamping component 3, supporting legs 12 are fixedly disposed at the lower surface corner of the bottom plate 1, a first guide groove 21 is disposed on one side of the side plate 2, a first filtering shell 5 and a second filtering shell 6 are slidably disposed in the first guide groove 21, a second filtering shell 6 is disposed on one side of the first filtering shell 5, an auxiliary component 7 is fixedly disposed on the upper surface of the first filtering shell 5, assembly components 8 are fixedly disposed on the surfaces of the two sides of the first filtering shell 5, and a telescopic component 9 is disposed below the second filtering shell 6, the lower fixed surface of first filter housing 5 and second filter housing 6 is provided with first spring 211, the upper fixed surface of second filter housing 6 is provided with pipeline 61, and the one end of this pipeline 61 is fixed to be set up in the upper surface of second filter housing 6, and the other end is fixed to be set up on the upper surface of first casing 5. When the cell culture device is used, firstly, a bottle containing cell matrixes and a bottle containing filtered cell matrixes are placed in the containing groove 11 formed in the upper surface of the bottom plate 1. At this time, the initial state of the clamping assembly 3 is shown in fig. 2, the slide bar 32 extends along the second guide groove 31 under the action of the second spring 33, and the clamping plate 34 tilts under the action of the third spring 35. When the bottle containing the cell base and the bottle containing the filtered cell base are placed in the accommodating groove 11, the bottle applies force to the clamping plate 34 to enable the clamping plate 34 and the sliding rod 32 fixedly arranged on the clamping plate to slide along the second guide groove 31, meanwhile, the second spring 33 and the fifth spring 35 clamp and fix the bottle containing the cell base and the bottle containing the filtered cell base through the clamping plate 34, in the process, the clamping assembly 3 is in an initial state, the third spring 35 enables the clamping plate 34 to incline to facilitate placement of the bottle, and the clamping surface of the clamping plate 34 is an arc surface to facilitate clamping and fixing of the bottle. When the bottle that will be equipped with the cell base and the bottle of dress cell base after filtering are put into storage tank 11, the bottle will exert force to grip block 34, make grip block 34 and slide bar 32 of fixed setting on it slide along second guide way 31, back slide bar 32 makes first transfer line 43 swing through the transmission groove 36 of its tip, first transfer line 43 swings and drives second transfer line 44 swing simultaneously, this auxiliary assembly 7 is passed with power to second transfer line 44 swing at last, this action structure, not only the action is swift to have improved the transformation that the use efficiency realized the motion form, and the structure is mostly the reliable long service life of low vice action. The second transmission rod 44 swings to transmit force to the sliding block 73 in the auxiliary assembly 7, so that the sliding block slides downwards along the guide block 71, meanwhile, the fourth spring 72 is extruded, the force is transmitted to the first filtering shell 5, the first filtering shell 5 moves downwards along the first guide groove 21 until the leakage shell 53 contacts the bottle mouth containing the filtered cell base, and in the action structure, the fourth spring 72 can reduce the impact generated when the transmission assembly 3 transmits, so that the bottle mouth containing the filtered cell base is protected. First filter housing 5 moves down along first guide way 21, when the weeping casing 53 contacts the bottle bottleneck of dress cell base after filtering, with first filter housing fixed connection's second filter housing 6 moves down, telescopic cylinder 91 extends to the bottle bottom, is provided with fifth spring 92 in telescopic cylinder 91's the outside, when telescopic cylinder 91 touched the bottle end, can take place to warp in order to realize the effect at the bottom of the protection bottle. The air pump 51 is started to suck the cell-based solution contained under the second filter housing 6 into the first filter housing 5, and then the cell-based solution is filtered by the filter screen 52, so that the filtered cell-based solution flows into the bottle under the first filter housing 5, and the filtration is completed. When the assembly component 8 of the invention is used, firstly, the assembly component 8 is pressed tightly as shown in fig. 3, when the filter screen 52 needs to be cleaned, the first swing block 85 is swung towards the axial direction of the first filter shell 5 manually, then the stop block 83 is rotated towards the axial direction of the first filter shell 5 manually by overcoming the torsion force of the torsion spring 84, then the first swing block 85 is put down, meanwhile, the second swing block 87 fixedly connected with the first swing block 85 swings, and moves downwards to separate the liquid leaking shell 53 and the filter screen 52 arranged on the liquid leaking shell 53 from the lower surface of the first filter shell 5, at this moment, the filter screen 52 is taken out from the side surface of the auxiliary component 8 for cleaning, after the cleaning is finished, the filter screen 52 is placed on the upper surface of the liquid leaking shell 53 again along one side of the auxiliary component 8, after the placement is finished, the first swing block 85 is shifted upwards to enable the liquid leaking shell 53 to move upwards to press the filter screen 52, the assembly is completed, the arrangement of the structure enables the filter screen 52 to be detached for cleaning, the filtering effect of the filter screen 52 is improved, and the structure is simple and the action is reliable and rapid.
In this embodiment, the clamping assembly 3 includes a second guide groove 31, the second guide groove 31 is opened at two sides of the accommodating groove 11, a slide bar 32 is slidably disposed in the second guide groove 31, a second spring 33 is fixedly disposed on a surface of one end of the slide bar 32, a clamping plate 34 is disposed at a swinging position of the other end of the slide bar 32, a third spring 35 is fixedly disposed on a surface of a lower portion of the clamping plate 34, one end of the third spring 35 is fixedly disposed on a side surface of the clamping plate 34, the other end of the third spring is fixedly disposed on an inner wall of the assembling groove 11, a transmission groove 36 is disposed at one end of the slide bar 32, a transmission assembly 4 is disposed in the transmission groove 36, a pipeline 61 is fixedly disposed on an upper surface of the second filter housing 6, one end of the pipeline 61 is fixedly disposed on an upper surface of the second filter housing 6, and the other end of the pipeline is fixedly disposed on an upper surface of the first filter housing 5. When the bottle containing the cell base and the bottle containing the filtered cell base are placed in the accommodating groove 11, the bottle applies force to the clamping plate 34 to enable the clamping plate 34 and the sliding rod 32 fixedly arranged on the clamping plate to slide along the second guide groove 31, meanwhile, the second spring 33 and the fifth spring 35 clamp and fix the bottle containing the cell base and the bottle containing the filtered cell base through the clamping plate 34, in the process, the clamping assembly 3 is in an initial state, the third spring 35 enables the clamping plate 34 to incline to facilitate placement of the bottle, and the clamping surface of the clamping plate 34 is an arc surface to facilitate clamping and fixing of the bottle.
In this embodiment, the transmission assembly 4 includes a support plate 41 and a support column 42, one side of the support plate 41 is provided with the support column 42, and the support plate 41 and the support column 42 are both fixedly arranged on the upper surface of the bottom plate 1, the support plate 41 is provided with a first transmission rod 43 in a rotating manner, one end of the support column 41 is provided with a second transmission rod 44 in a rotating manner, the end of the first transmission rod 43 is arranged in the transmission groove 36, and the other end of the first transmission rod is arranged in the middle of the second transmission rod 44. When the bottle that will be equipped with the cell base and the bottle of dress cell base after filtering are put into storage tank 11, the bottle will exert force to grip block 34, make grip block 34 and slide bar 32 of fixed setting on it slide along second guide way 31, back slide bar 32 makes first transfer line 43 swing through the transmission groove 36 of its tip, first transfer line 43 swings and drives second transfer line 44 swing simultaneously, this auxiliary assembly 7 is passed with power to second transfer line 44 swing at last, this action structure, not only the action is swift to have improved the transformation that the use efficiency realized the motion form, and the structure is mostly the reliable long service life of low vice action.
In this embodiment, the auxiliary assembly 7 includes a guide block 71, the guide block 71 is disposed on the upper surface of the first filter housing 5, a fourth spring 72 is fixedly disposed on the lower bottom surface of the guide block 71, a slider 73 is fixedly disposed on the upper surface of the fourth spring 72, and the slider 73 is slidably disposed on the guide block 71. The second transmission rod 44 swings to transmit force to the sliding block 73 in the auxiliary assembly 7, so that the sliding block slides downwards along the guide block 71, meanwhile, the fourth spring 72 is extruded, the force is transmitted to the first filtering shell 5, the first filtering shell 5 moves downwards along the first guide groove 21 until the leakage shell 53 contacts the bottle mouth containing the filtered cell base, and in the action structure, the fourth spring 72 can reduce the impact generated when the transmission assembly 3 transmits, so that the bottle mouth containing the filtered cell base is protected.
In this embodiment, an air pump 51 is fixedly disposed on the upper surface of the first filter housing 5, a filter screen 52 is disposed below the first filter housing 5, a liquid leaking housing 53 is disposed below the filter screen 52, and the edge of the filter screen 52 is made of a rubber material. The edge of the filter screen 52 is made of rubber materials, which is beneficial to sealing.
In this embodiment, the assembling component 8 includes a first fixing block 81 and a second fixing block 82, the second fixing block 82 is disposed below the first fixing block 81, the first fixing block 81 is fixedly disposed on a side surface of the first filtering casing 5, the second fixing block 82 is fixedly disposed on the liquid leaking casing 53, a stopper 83 is rotatably disposed on the first fixing block 81, a torsion spring 84 is disposed on the stopper 83, a first swing block 85 is disposed on an inner side of the stopper 83, the first swing block 85 is rotatably disposed on the first fixing block 81, a rotating shaft 86 is disposed on the second fixing block 82, a second swing block 87 is disposed on the rotating shaft 86, and an upper end of the second swing block 87 is disposed on one side of the first swing block 85. When the filter screen 52 needs to be cleaned, the first swing block 85 is manually swung towards the axial direction of the first filter shell 5, then the stop block 83 is manually rotated towards the axial direction of the first filter shell 5 by overcoming the torsion of the torsion spring 84, then the first swing block 85 is put down, meanwhile, the second swing block 87 fixedly connected to the first swing block 85 swings, the liquid leaking shell 53 and the filter screen 52 placed on the liquid leaking shell 53 are separated from the lower surface of the first filter shell 5 by moving downwards, at the moment, the filter screen 52 is taken out from the side surface of the auxiliary assembly 8 for cleaning, after the cleaning is finished, the filter screen 52 is placed on the liquid leaking shell 53 again along one side of the auxiliary assembly 8, after the placement is finished, the first swing block 85 is shifted upwards, the liquid leaking shell 53 is moved upwards to press the filter screen 52, the assembly is finished, the structure enables the filter screen 52 to be detached for cleaning, the filtering effect of the filter screen 52 is improved, the structure is simple, and the operation is reliable and quick.
In this embodiment, the telescopic assembly 9 includes a telescopic cylinder 91, the telescopic cylinder 91 is slidably disposed below the second filtering housing 6, a fifth spring 92 is disposed outside the telescopic cylinder 91, and a sealing ring 93 is disposed at an upper end of the telescopic cylinder 91. First filter housing 5 moves down along first guide way 21, when the weeping casing 53 contacts the bottle bottleneck of dress cell base after filtering, with first filter housing fixed connection's second filter housing 6 moves down, telescopic cylinder 91 extends to the bottle bottom, is provided with fifth spring 92 in telescopic cylinder 91's the outside, when telescopic cylinder 91 touched the bottle end, can take place to warp in order to realize the effect at the bottom of the protection bottle.
The invention has the beneficial effects that:
when the bottle with the cell base and the bottle with the filtered cell base are placed in the accommodating groove 11, the bottle applies force to the clamping plate 34 to enable the clamping plate 34 and the sliding rod 32 fixedly arranged on the clamping plate to slide along the second guide groove 31, meanwhile, the second spring 33 and the fifth spring 35 clamp and fix the bottle with the cell base and the bottle with the filtered cell base through the clamping plate 34, in the process, the clamping assembly 3 is in an initial state, the third spring 35 enables the clamping plate 34 to incline to facilitate placement of the bottle, and the clamping surface of the clamping plate 34 is an arc surface to facilitate clamping and fixing of the bottle;
when the bottles filled with cell matrixes and the filtered cell matrixes are placed in the accommodating groove 11, the bottles apply force to the clamping plate 34 to enable the clamping plate 34 and the sliding rod 32 fixedly arranged on the clamping plate to slide along the second guide groove 31, the rear sliding rod 32 enables the first transmission rod 43 to swing through the transmission groove 36 at the end part of the rear sliding rod 32, the first transmission rod 43 swings and simultaneously drives the second transmission rod 44 to swing, and finally the second transmission rod 44 swings to transmit the force to the auxiliary assembly 7, so that the action structure not only can quickly act and improve the use efficiency and realize the change of the movement form, but also mostly has low-pair action, reliability and long service life;
the second transmission rod 44 swings to transmit force to the sliding block 73 in the auxiliary assembly 7, so that the sliding block slides downwards along the guide block 71, meanwhile, the fourth spring 72 is extruded, the force is transmitted to the first filtering shell 5, the first filtering shell 5 moves downwards along the first guide groove 21 until the leakage shell 53 contacts the bottle mouth containing the filtered cell base, and in the action structure, the fourth spring 72 can reduce the impact generated when the transmission assembly 3 transmits, so that the bottle mouth containing the filtered cell base is protected;
when the filter screen 52 needs to be cleaned, the first swing block 85 is manually swung towards the axial direction of the first filter housing 5, then the stop block 83 is manually rotated towards the axial direction of the first filter housing 5 by overcoming the torsion of the torsion spring 84, then the first swing block 85 is put down, meanwhile, the second swing block 87 fixedly connected with the first swing block 85 swings, the first swing block moves downwards to separate the liquid leaking housing 53 and the filter screen 52 placed on the liquid leaking housing 53 from the lower surface of the first filter housing 5, at the moment, the filter screen 52 is taken out from the side surface of the auxiliary assembly 8 for cleaning, after the cleaning is finished, the filter screen 52 is placed on the liquid leaking housing 53 again along one side of the auxiliary assembly 8, after the placement is finished, the first swing block 85 is shifted upwards to move the liquid leaking housing 53 upwards to compress the filter screen 52, the assembly is finished, the structure enables the filter screen 52 to be detached for cleaning, and the filtering effect of the filter screen 52 is improved, the structure is simple, and the operation is reliable and quick.
First filter housing 5 moves down along first guide way 21, when the weeping casing 53 contacts the bottle bottleneck of dress cell base after filtering, with first filter housing fixed connection's second filter housing 6 moves down, telescopic cylinder 91 extends to the bottle bottom, is provided with fifth spring 92 in telescopic cylinder 91's the outside, when telescopic cylinder 91 touched the bottle end, can take place to warp in order to realize the effect at the bottom of the protection bottle.
The working principle is as follows:
when the cell culture device is used, firstly, a bottle containing cell matrixes and a bottle containing filtered cell matrixes are placed in the containing groove 11 formed in the upper surface of the bottom plate 1. At this time, the initial state of the clamping assembly 3 is shown in fig. 2, the slide bar 32 extends along the second guide groove 31 under the action of the second spring 33, and the clamping plate 34 tilts under the action of the third spring 35. When the bottle containing the cell base and the bottle containing the filtered cell base are placed in the accommodating groove 11, the bottle applies force to the clamping plate 34 to enable the clamping plate 34 and the sliding rod 32 fixedly arranged on the clamping plate to slide along the second guide groove 31, meanwhile, the second spring 33 and the fifth spring 35 clamp and fix the bottle containing the cell base and the bottle containing the filtered cell base through the clamping plate 34, in the process, the clamping assembly 3 is in an initial state, the third spring 35 enables the clamping plate 34 to incline to facilitate placement of the bottle, and the clamping surface of the clamping plate 34 is an arc surface to facilitate clamping and fixing of the bottle. When the bottle that will be equipped with the cell base and the bottle of dress cell base after filtering are put into storage tank 11, the bottle will exert force to grip block 34, make grip block 34 and slide bar 32 of fixed setting on it slide along second guide way 31, back slide bar 32 makes first transfer line 43 swing through the transmission groove 36 of its tip, first transfer line 43 swings and drives second transfer line 44 swing simultaneously, this auxiliary assembly 7 is passed with power to second transfer line 44 swing at last, this action structure, not only the action is swift to have improved the transformation that the use efficiency realized the motion form, and the structure is mostly the reliable long service life of low vice action. The second transmission rod 44 swings to transmit force to the sliding block 73 in the auxiliary assembly 7, so that the sliding block slides downwards along the guide block 71, meanwhile, the fourth spring 72 is extruded, the force is transmitted to the first filtering shell 5, the first filtering shell 5 moves downwards along the first guide groove 21 until the leakage shell 53 contacts the bottle mouth containing the filtered cell base, and in the action structure, the fourth spring 72 can reduce the impact generated when the transmission assembly 3 transmits, so that the bottle mouth containing the filtered cell base is protected. First filter housing 5 moves down along first guide way 21, when the weeping casing 53 contacts the bottle bottleneck of dress cell base after filtering, with first filter housing fixed connection's second filter housing 6 moves down, telescopic cylinder 91 extends to the bottle bottom, is provided with fifth spring 92 in telescopic cylinder 91's the outside, when telescopic cylinder 91 touched the bottle end, can take place to warp in order to realize the effect at the bottom of the protection bottle. Starting the air pump 51 to suck the cell-based solution contained under the second filter housing 6 into the first filter housing 5, and then filtering through the filter screen 52, wherein the filtered cell-based solution flows into the bottle under the first filter housing 5 to complete the filtering. When the assembly component 8 of the invention is used, firstly, the assembly component 8 is in a compressed state as shown in fig. 3, when the filter screen 52 needs to be cleaned, the first swing block 85 is swung towards the axial direction of the first filter shell 5 manually, then the stop block 83 is rotated towards the axial direction of the first filter shell 5 manually by overcoming the torsion of the torsion spring 84, then the first swing block 85 is put down, meanwhile, the second swing block 87 fixedly connected with the first swing block 85 swings, and moves downwards to separate the liquid leaking shell 53 and the filter screen 52 arranged on the liquid leaking shell 53 from the lower surface of the first filter shell 5, at this moment, the filter screen 52 is taken out from the side surface of the auxiliary component 8 for cleaning, after the cleaning is finished, the filter screen 52 is placed on the upper surface of the liquid leaking shell 53 again along one side of the auxiliary component 8, after the placement is finished, the first swing block 85 is stirred upwards to enable the liquid leaking shell 53 to move upwards to compress the filter screen 52, the assembly is completed, the arrangement of the structure enables the filter screen 52 to be detached for cleaning, the filtering effect of the filter screen 52 is improved, and the structure is simple and the action is reliable and rapid.
The embodiments of the present invention are disclosed as the preferred embodiments, but not limited thereto, and those skilled in the art can easily understand the spirit of the present invention and make various extensions and changes without departing from the spirit of the present invention.
Claims (7)
1. A tangential flow filter based on cell culture comprises a bottom plate (1) and a side plate (2), wherein the side plate (2) is vertically arranged on one side of the bottom plate (1), and is characterized in that a containing groove (11) is formed in the upper surface of the bottom plate (1), a clamping assembly (3) is arranged in the containing groove (11) in a sliding manner, a transmission assembly (4) is arranged on one side of the clamping assembly (3) in a swinging manner, supporting legs (12) are fixedly arranged at the edge of the lower surface of the bottom plate (1), a first guide groove (21) is formed in one side of the side plate (2), a first filtering shell (5) and a second filtering shell (6) are arranged in the first guide groove (21) in a sliding manner, a second filtering shell (6) is arranged on one side of the first filtering shell (5), an auxiliary assembly (7) is fixedly arranged on the upper surface of the first filtering shell (5), the utility model discloses a filter, including first filter casing (5), second filter casing (6), first both sides fixed surface of filtering casing (5) is provided with assembly subassembly (8), be provided with below second filter casing (6) flexible subassembly (9), the lower fixed surface of first filter casing (5) and second filter casing (6) is provided with first spring (211), the last fixed surface of second filter casing (6) is provided with pipeline (61), and the one end of this pipeline (61) is fixed to be set up in the upper surface of second filter casing (6), and the other end is fixed to be set up on the upper surface of first casing (5).
2. A cell culture based tangential flow filter according to claim 1, wherein: the clamping assembly (3) comprises a second guide groove (31), the second guide groove (31) is arranged on two sides of the accommodating groove (11), a slide rod (32) is arranged in the second guide groove (31) in a sliding manner, a second spring (33) is fixedly arranged on one end surface of the slide rod (32), a clamping plate (34) is arranged at the other end in a swinging manner, a third spring (35) is fixedly arranged on the lower portion surface of the clamping plate (34), one end of the third spring (35) is fixedly arranged on the side surface of the clamping plate (34), the other end of the third spring is fixedly arranged on the inner wall of the assembling groove (11), a transmission groove (36) is formed in one end of the slide rod (32), a transmission assembly (4) is arranged in the transmission groove (36), a pipeline (61) is fixedly arranged on the upper surface of the second filtering shell (6), one end of the pipeline (61) is fixedly arranged on the upper surface of the second filtering shell (6), the other end is fixedly arranged on the upper surface of the first filtering shell (5).
3. A cell culture based tangential flow filter according to claim 2, wherein: drive assembly (4) are including backup pad (41) and support column (42), one side of backup pad (41) is provided with support column (42), and backup pad (41) and support column (42) all fixed the setting in the upper surface of bottom plate (1), it is provided with first transfer line (43) to rotate on backup pad (41), the one end of support column (41) is rotated and is provided with second transfer line (44), the tip of first transfer line (43) sets up in drive groove (36), and the other end sets up in second transfer line (44) middle part.
4. A cell culture based tangential flow filter according to claim 3, wherein: the auxiliary assembly (7) comprises a guide block (71), the guide block (71) is arranged on the upper surface of the first filtering shell (5), a fourth spring (72) is fixedly arranged on the lower bottom surface of the guide block (71), a sliding block (73) is fixedly arranged on the upper surface of the fourth spring (72), and the sliding block (73) is arranged on the guide block (71) in a sliding manner.
5. A cell culture based tangential flow filter according to claim 4, wherein: the upper surface of the first filtering shell (5) is fixedly provided with an air pump (51), a filtering net (52) is arranged below the first filtering shell (5), a liquid leakage shell (53) is arranged below the filtering net (52), and the edge of the filtering net (52) is made of rubber materials.
6. A cell culture based tangential flow filter according to claim 5, wherein: the assembly component (8) comprises a first fixing block (81) and a second fixing block (82), the second fixing block (82) is arranged below the first fixing block (81), the first fixing block (81) is fixedly arranged on the side face of the first filtering shell (5), the second fixing block (82) is fixedly arranged on the liquid leakage shell (53), a stop block (83) is arranged on the first fixing block (81) in a rotating mode, a torsion spring (84) is arranged on the stop block (83), a first swing block (85) is arranged on the inner side of the stop block (83), the first swing block (85) is arranged on the first fixing block (81) in a rotating mode, a rotating shaft (86) is arranged on the second fixing block (82), a second swing block (87) is arranged on the rotating shaft (86), and the upper end of the second swing block (87) is arranged on one side of the first swing block (85).
7. A cell culture based tangential flow filter according to claim 6, wherein: the telescopic assembly (9) comprises a telescopic cylinder (91), the telescopic cylinder (91) is arranged below the second filtering shell (6) in a sliding mode, a fifth spring (92) is arranged on the outer side of the telescopic cylinder (91), and a sealing ring (93) is arranged at the upper end of the telescopic cylinder (91).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210332265.6A CN114634863A (en) | 2022-03-30 | 2022-03-30 | Tangential flow filter based on cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210332265.6A CN114634863A (en) | 2022-03-30 | 2022-03-30 | Tangential flow filter based on cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114634863A true CN114634863A (en) | 2022-06-17 |
Family
ID=81952465
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210332265.6A Pending CN114634863A (en) | 2022-03-30 | 2022-03-30 | Tangential flow filter based on cell culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114634863A (en) |
Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106190810A (en) * | 2016-08-30 | 2016-12-07 | 浙江泰林生物技术股份有限公司 | A kind of membrane filtration sterility test device |
| CN107876115A (en) * | 2016-09-30 | 2018-04-06 | 广州康昕瑞基因健康科技有限公司 | Anticollision liquid-transfering needle |
| CN208151386U (en) * | 2018-01-30 | 2018-11-27 | 卡替（上海）生物技术股份有限公司 | A kind of deciduous teeth dental pulp stem cell saturated humidity incubator |
| CN109979538A (en) * | 2019-03-28 | 2019-07-05 | 广州基迪奥生物科技有限公司 | A kind of analysis method based on the unicellular transcript profile sequencing data of 10X |
| CN210029990U (en) * | 2019-04-22 | 2020-02-07 | 李杰慧 | Medical liquid medicine split charging device |
| CN210708688U (en) * | 2019-10-12 | 2020-06-09 | 宁波金雨科技实业有限公司 | Dropper capable of preventing liquid in bottle from remaining |
| CN211401807U (en) * | 2019-12-03 | 2020-09-01 | 肖峰 | Novel medical liquid taking device |
| CN211568683U (en) * | 2019-12-08 | 2020-09-25 | 丁雪松 | Fixing device with good using effect |
| CN111849779A (en) * | 2020-07-09 | 2020-10-30 | 中国人民解放军空军军医大学 | Filter equipment for cell culture |
| CN212054933U (en) * | 2020-04-28 | 2020-12-01 | 江苏星辰星汽车附件有限公司 | Mounting and positioning fixture for automobile air filter |
| CN112173382A (en) * | 2020-09-18 | 2021-01-05 | 丁亚宁 | Low-temperature box for cold-chain logistics transportation of medical articles and use method thereof |
| CN113022911A (en) * | 2021-03-03 | 2021-06-25 | 上海乐纯生物技术有限公司 | Split charging system |
| CN215350354U (en) * | 2021-06-25 | 2021-12-31 | 石家庄美然医疗美容服务有限公司 | Head fixing device for plastic cosmetology department |
| CN114602239A (en) * | 2022-03-31 | 2022-06-10 | 上海格氏流体设备科技有限公司 | Tangential flow intelligent filtering device for biological pharmacy |
-
2022
- 2022-03-30 CN CN202210332265.6A patent/CN114634863A/en active Pending
Patent Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106190810A (en) * | 2016-08-30 | 2016-12-07 | 浙江泰林生物技术股份有限公司 | A kind of membrane filtration sterility test device |
| CN107876115A (en) * | 2016-09-30 | 2018-04-06 | 广州康昕瑞基因健康科技有限公司 | Anticollision liquid-transfering needle |
| CN208151386U (en) * | 2018-01-30 | 2018-11-27 | 卡替（上海）生物技术股份有限公司 | A kind of deciduous teeth dental pulp stem cell saturated humidity incubator |
| CN109979538A (en) * | 2019-03-28 | 2019-07-05 | 广州基迪奥生物科技有限公司 | A kind of analysis method based on the unicellular transcript profile sequencing data of 10X |
| CN210029990U (en) * | 2019-04-22 | 2020-02-07 | 李杰慧 | Medical liquid medicine split charging device |
| CN210708688U (en) * | 2019-10-12 | 2020-06-09 | 宁波金雨科技实业有限公司 | Dropper capable of preventing liquid in bottle from remaining |
| CN211401807U (en) * | 2019-12-03 | 2020-09-01 | 肖峰 | Novel medical liquid taking device |
| CN211568683U (en) * | 2019-12-08 | 2020-09-25 | 丁雪松 | Fixing device with good using effect |
| CN212054933U (en) * | 2020-04-28 | 2020-12-01 | 江苏星辰星汽车附件有限公司 | Mounting and positioning fixture for automobile air filter |
| CN111849779A (en) * | 2020-07-09 | 2020-10-30 | 中国人民解放军空军军医大学 | Filter equipment for cell culture |
| CN112173382A (en) * | 2020-09-18 | 2021-01-05 | 丁亚宁 | Low-temperature box for cold-chain logistics transportation of medical articles and use method thereof |
| CN113022911A (en) * | 2021-03-03 | 2021-06-25 | 上海乐纯生物技术有限公司 | Split charging system |
| CN215350354U (en) * | 2021-06-25 | 2021-12-31 | 石家庄美然医疗美容服务有限公司 | Head fixing device for plastic cosmetology department |
| CN114602239A (en) * | 2022-03-31 | 2022-06-10 | 上海格氏流体设备科技有限公司 | Tangential flow intelligent filtering device for biological pharmacy |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN209021914U (en) | A kind of anti-offset drive disk multi-functional clamp | |
| CN114634863A (en) | Tangential flow filter based on cell culture | |
| CN204903158U (en) | Ball valve test machine of gas tightness of holding concurrently test and torsion -testing | |
| CN214716694U (en) | Novel coronavirus's sealed transfer case | |
| CN207977396U (en) | A kind of Soft Roll power battery vacuum liquid filling mechanism | |
| CN211501481U (en) | Fixing device of full-automatic nucleic acid extraction appearance | |
| CN213456246U (en) | Medical treatment clinical laboratory uses reagent sample operation panel | |
| CN113956975A (en) | Cell detection is with device of adding culture solution in many forms reagent box | |
| CN220779750U (en) | Sol device for hard capsule production | |
| CN208055366U (en) | A kind of cell climbing sheet holder | |
| CN208194298U (en) | A kind of clinical laboratory's humor collecting feeding device for inspection | |
| CN218989244U (en) | Multichannel bioreactor | |
| CN214420043U (en) | Pen point spring embedding mechanism | |
| CN218218682U (en) | Portable portable tissue culture frame | |
| CN213652523U (en) | Shaking and vibrating machine special for stem cell culture | |
| CN220277043U (en) | Test tube upset liquid mechanism of falling | |
| CN221025102U (en) | Detection sampling box | |
| CN209155656U (en) | Integral type silica gel experimental facilities | |
| CN212688034U (en) | Microbial nucleic acid detection kit | |
| CN214311189U (en) | Automatic gluing device | |
| CN219099116U (en) | Full-automatic trace DNA extraction element with shake even function | |
| CN219297475U (en) | Culture bottle oscillating device capable of adjusting clamping size | |
| CN220483967U (en) | Transfer device | |
| CN220201894U (en) | Multifunctional magnetic rack | |
| CN216965574U (en) | Wiper mechanism is used in production of cell culture apparatus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220617 |