CN109431854A - A kind of bFGF acne hole maintenance freeze-dried powder preparation and preparation method thereof - Google Patents
A kind of bFGF acne hole maintenance freeze-dried powder preparation and preparation method thereof Download PDFInfo
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- CN109431854A CN109431854A CN201811487234.8A CN201811487234A CN109431854A CN 109431854 A CN109431854 A CN 109431854A CN 201811487234 A CN201811487234 A CN 201811487234A CN 109431854 A CN109431854 A CN 109431854A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/0216—Solid or semisolid forms
- A61K8/022—Powders; Compacted Powders
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/46—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing sulfur
- A61K8/466—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing sulfur containing sulfonic acid derivatives; Salts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/10—Anti-acne agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/88—Two- or multipart kits
- A61K2800/882—Mixing prior to application
Abstract
The present invention relates to cosmetic technical fields, specifically a kind of bFGF acne hole maintenance freeze-dried powder preparation and preparation method thereof, including cheating the solution that maintenance freeze-dried powder and bFGF acne hole maintenance lyase liquid are constituted by bFGF acne, wherein, bFGF acne hole maintenance freeze-dried powder is made by the component of following quality proportionings: oligopeptides -3, mannitol, Sodium Hyaluronate, oligopeptides -1, water；BFGF acne hole maintenance freeze-dried powder lyase is made by the component of following quality proportionings: human-like collagen, collagen victory peptide, dipropylene glycol, propylene glycol, glycerol, sodium azulenesulfonate solution, GC-04, water；The two of task of the present invention are to provide a kind of preparation method of bFGF acne hole maintenance freeze-dried powder preparation.
Description
Technical field
The present invention relates to cosmetic technical field, specifically a kind of bFGF acne hole maintenance freeze-dried powder preparation and its preparation side
Method.
Background technique
Acne hole is a kind of face's disease due to caused by infection inflammation or external force extruding, and most important performance is the hair of face
All concave point point inwards around hole, shape is as orange peel, and pore is coarse, and is that each pore is cheated with regard to one.It generates
Other reasons: the pollution of diet, environment usually, endocrine disorder, mood is fidgety, uses bad cosmetics.
Summary of the invention
In order to overcome the above the deficiencies in the prior art, one of task of the invention is to provide a kind of bFGF acne hole maintenance freeze-drying
Powder preparation, a kind of bFGF acne cheat maintenance freeze-dried powder preparation and solve acne hole, acne print, obstinate acne flesh from profound level, and improvement skin quality mentions
The effect of skin lightening matter, antiallergy, triggering flesh bottom vigor；The two of task of the present invention are to provide a kind of bFGF acne hole maintenance freeze-dried powder system
The preparation method of agent.
One of task of the present invention is achieved through the following technical solutions:
A kind of bFGF acne hole maintenance freeze-dried powder preparation, including maintenance freeze-dried powder and bFGF acne hole maintenance lyase are cheated by bFGF acne
The solution that liquid is constituted, wherein bFGF acne hole maintenance freeze-dried powder is made by the component of following quality proportionings:
Oligopeptides -3:0.5%-1%；
Mannitol: 5%-15%；
Sodium Hyaluronate: 0.02%-0.05%；
Oligopeptides -1:0.2%-0.5%；
Water: 83.45%-94.28%；
Oligopeptides -3 (Basic Fibroblast Growth Factor (abbreviation bFGF)) is existing in mammal and human body in the present invention
BFGF receptor is distributed in a kind of micro substance in Various Tissues cell, thus its physiological action is extensive, is a kind of multi-functional thin
The intracellular growth factor.
Mannitol is conducive to the conditioning of skin and anti-ageing to the elimination of peroxynitrite in the present invention, to skin
Allergy also has a degree of inhibiting effect；Mannitol can inhibit the light injury and light aging to skin, and have moisture-keeping functions.
Sodium Hyaluronate is also known as sodium hyaluronate sodium in the present invention, belongs to skin conditioner, is a kind of acid mucopolysaccharide, naturally deposits
It is in cornea skin, can absorb itself 1000 times of moisture of weight, reaches by retaining moisture of skin, preventing moisture through epidermis
It is lost, and the barrier reparation when skin is damaged, skin is enabled not feel drying after, increase gloss, it can be with
Improve skin-nourishing metabolism, make skin it is soft, it is smooth, go wrinkle, increase elasticity, prevent aging, be good again while moisturizing
Skin penetration enhancer.It is used cooperatively with other nutritional ingredients, the more preferable effect for promoting nutrient absorption can be played.
EGF is also known as oligopeptides -1 in the present invention, is a kind of intracorporal active material of people, and the activity being made of 53 amino is more
Peptide.The Proliferation, Differentiation that can promote cell can be done directly on the generation that basal cell accelerates healthy neoblast, thus impaired
Keratinocyte layer be quickly ejected, form the neat cuticula of queueing discipline of health.EGF can be formed with enzymes various in human body
Good coordinating effect.Initial EGF is mainly employed for medical domain, be primarily to facilitate the reparation of impaired epidermis with again
It is raw, such as treatment burn, scald.
Water is solvent in the present invention；
BFGF acne hole maintenance freeze-dried powder lyase liquid is made by the component of following quality proportionings:
Human-like collagen: 1%-2%；
Collagen wins peptide: 0.5%-1%；
Dipropylene glycol: 3.8%-4.2%；
Propylene glycol: 1.8%-2.1%；
Glycerol: 1.8%-2.2%；
Sodium azulenesulfonate solution: 3%-5%；
GC-04:0.4-0.6%；
Water: 82.9%-87.7%；
Human-like collagen (Human-like Collagen) in the present invention is by biotechnology by human body glue
The mRNA reverse transcription of former albumen is at cDNA, and for one section of genetic recombination after digestion in E.coli (Escherichia coli), process is highly dense
Spend a kind of high-molecular biologic albumen of fermentation, separation, renaturation, purifying process production.Collagen is that people's in-vivo content is most
Protein is primarily present in skin, bone, cartilage, tendon, ligament and the blood vessel of human body, is of crucial importance in connective tissue
Structural protein, play support organ, protect the function of human body.It can be divided according to function and its design feature for constituting tissue
At: at fiber collagen, reticulate structure collagen, positioned at the fiber correlation collagen of fiber surface, it is former at beading silk gum, at flesh counterdie
Anchoring fiber collagen has transmembrane structure collagen etc..
Collagen in the present invention wins peptide (INCI: HYDROLYZED COLLAGEN/DIPEPTIDE-1, Chinese name
Claim: hydrolytic collagen/dipeptides -1), it is a kind of high molecular functional protein, it is the chief component of skin, and it is true to account for skin
The specific gravity of cortex 80%, it constitutes a fine and closely woven elasticated net in skin, securely locks moisture, and supporter's skin is rich in
The collagens characteristic amino acids such as glycine, proline, hydroxyl paving propylhomoserin that human body needs, are human body cell especially skin cells
Important component in epimatrix.This composite collagen victory peptide product has the following characteristics that the water conservation of 1, increase cuticula
Power enhances the tender degree of skin smooth；2, fine film can be formed on the skin, there is the effect of Firm at once；3, it directly permeates
To dermal layer of the skin, skin fibr tissue mechanism is reformed, promotes cell metabolism, delaying cell aging obtains fine wrinkles
To releiving；4, the water and oil balance for keeping skin, it is vigorous to avoid the occurrence of fat secretion；5, promote eye circumference to enclose skin microcirculation, reinforce
Eye skin metabolism improves eye pouch and black eye；6, stimulation cell division is proliferated, damaged cell is repaired in differentiation, healing wound
Mouthful；7, the injury of ultraviolet light (UVB) to skin is reduced.
Dipropylene glycol in the present invention has good moisture, and oil and hydrocarbon are total to molten ability, and smell is light
Micro-, to skin irritation very little, isomer is evenly distributed unanimously, and quality is splendid.
Propylene glycol in the present invention can be used as wetting agent with glycerol or sorbierite cooperation.
Glycerol in the present invention is colourless sweet clear and bright thick liquid.It is odorless.There is warm sweet taste.It can be used as solvent, lubricant,
Medicament and sweetener.
Sodium azulenesulfonate solution in the present invention is prepared by forming for following quality proportionings, sodium azulenesulfonate 0.45-
0.55% (No. CAS: 916445-22-2), axenic purification water 99.45-99.55%.Its effect has: 1, natural anti-inflammatory, anti-mistake
It is quick；2, aqueous easy absorption, sodium gualenate aqueous solution has inflammation caused by various control many kinds of substance, and effect is more lasting,
Inhibit inflammatory cell to release histamine by part directly effect, inhibits the synthesis of prostaglandin E2 in mucous membrane, granulation is promoted to be formed
With epithelial cell new life；3, ultraviolet light is effectively antagonized, the anti-UV activity index of sodium azulenesulfonate solution is higher, and water solubility is not added
The uvioresistant activity index of group is lower.
GC-04 extract in the present invention includes hydroxy acetophenone, glycerol caprylate and decoyl hydroximic acid, is a kind of natural
Plant extracts, can be described as no added preservative, and parahydroxyacet-ophenone is present in compositae plant artemisia scoparia (Artemisia
Scoparia Waldst.et kit.) stem, leaf and the anti-wormwood artemisia of mattress (A.capillaris Thunb.), Luo Mo section plant person
Join in the plants such as the root of radix tylophorae (Tylophora Kerrii Craib)；The pure natural glyceride type of glycerol caprylate is multi-functional to be replaced
It is rapeseed oil and cocounut oil derivative for property preservative, the mild polarity of skin is good, inhibits bacterium to agree yeast increment activity, also has
Moisturizing, emulsification, moisturizing, wetting and Sage Extract and other effects；Decoyl hydroximic acid is a no added preservative, it can be sterilized
Antibacterial, solubilized hydrotropy.
Water in the present invention is solvent.
The two of task of the present invention realize by following technical solution:
A kind of preparation method of above-mentioned bFGF acne hole maintenance freeze-dried powder preparation, the system including bFGF acne hole maintenance freeze-dried powder
The preparation method of Preparation Method and bFGF acne hole maintenance freeze-dried powder lyase liquid, wherein bFGF acne cheats the preparation method packet of maintenance freeze-dried powder
Include following steps:
S1: it the selection of raw material and prepares ratio: choosing the tap water by distillation and sterilization processing, mannitol,
BFGF, EGF are before carrying out mixing match in vacuum packaging, Sodium Hyaluronate constant temperature packaging；
S2: mannitol, the 0.05%-0.2% of bFGF, 5%-15% of 0.5%-1% are weighed by the quality proportioning
Sodium Hyaluronate, EGF, surplus water；
S3: bFGF, mannitol, Sodium Hyaluronate, EGF are placed in water, then mixture is placed in mixing plant
It is at the uniform velocity stirred, is to stir 10min in 600r/min in revolving speed, stirring forms mixed solution A；
S4: mixed solution A standing being again placed in mixing plant afterwards for 24 hours and is at the uniform velocity stirred, and is 1200r/ in revolving speed
20min is stirred in min；
S5: mixed solution A is filtered by filter membrane, is removed impurity not soluble in water in mixed solution A and is mixed
Close solution B；
S6: mixed solution B being placed in centrifugation apparatus and carries out centrifugally operated, then carries out precooling operation, at this time temperature
Control carries out refrigeration operation, mixed solution B lyophilization after the completion of -5 DEG C, precooling, and temperature is controlled at -15 DEG C.
S7: the mixed solution after lyophilization be placed in sterilizing vacuum bag be vacuum-packed, storage temperature control 0 DEG C with
Under；
Distillation tap water temperature in S1 is controlled at 50-70 DEG C；By the filter membrane diameter control in S5 in 0.1um by S6
Powder C need to be made in mixed liquor after lyophilization after ultraviolet sterilization；
Wherein bFGF acne hole maintenance freeze-dried powder lyase liquid preparation method the following steps are included:
S1, human-like collagen, collagen victory peptide stirring and dissolving to transparent, obtained material A are weighed by quality proportioning；
S2, the water stirring of material A and dipropylene glycol, propylene glycol, glycerol, sodium azulenesulfonate solution, GC-04, surplus is divided
It dissipates, is dispersed with stirring within the scope of 2.0-5.0Hz with dispersion machine to uniform, then through 150-250 mesh net filtration, bFGF is made
Acne cheats maintenance freeze-dried powder lyase liquid D, and powder C is mixed in bFGF acne hole maintenance freeze-dried powder lyase liquid D, obtains the hole maintenance of bFGF acne
Freeze-dried powder preparation.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that saying that the specific embodiment of description is used only for explaining the present invention herein, it is not used to
Limit the present invention.
A kind of bFGF acne hole maintenance freeze-dried powder preparation, including maintenance freeze-dried powder and bFGF acne hole maintenance lyase are cheated by bFGF acne
The solution that liquid is constituted, wherein bFGF acne hole maintenance freeze-dried powder is made by the component of following quality proportionings:
Oligopeptides -3:0.5%-1%；
Mannitol: 5%-15%；
Sodium Hyaluronate: 0.02%-0.05%；
Oligopeptides -1:0.2%-0.5%；
Water: 83.45%-94.28%；
Oligopeptides -3 (Basic Fibroblast Growth Factor (abbreviation bFGF)) is existing in mammal and human body in the present invention
BFGF receptor is distributed in a kind of micro substance in Various Tissues cell, thus its physiological action is extensive, is a kind of multi-functional thin
The intracellular growth factor.
Mannitol is conducive to the conditioning of skin and anti-ageing to the elimination of peroxynitrite in the present invention, to skin
Allergy also has a degree of inhibiting effect；Mannitol can inhibit the light injury and light aging to skin, and have moisture-keeping functions.
Sodium Hyaluronate is also known as sodium hyaluronate sodium in the present invention, belongs to skin conditioner, is a kind of acid mucopolysaccharide, naturally deposits
It is in cornea skin, can absorb itself 1000 times of moisture of weight, reaches by retaining moisture of skin, preventing moisture through epidermis
It is lost, and the barrier reparation when skin is damaged, skin is enabled not feel drying after, increase gloss, it can be with
Improve skin-nourishing metabolism, make skin it is soft, it is smooth, go wrinkle, increase elasticity, prevent aging, be good again while moisturizing
Skin penetration enhancer.It is used cooperatively with other nutritional ingredients, the more preferable effect for promoting nutrient absorption can be played.
EGF is also known as oligopeptides -1 in the present invention, is a kind of intracorporal active material of people, and the activity being made of 53 amino is more
Peptide.The Proliferation, Differentiation that can promote cell can be done directly on the generation that basal cell accelerates healthy neoblast, thus impaired
Keratinocyte layer be quickly ejected, form the neat cuticula of queueing discipline of health.EGF can be formed with enzymes various in human body
Good coordinating effect.Initial EGF is mainly employed for medical domain, be primarily to facilitate the reparation of impaired epidermis with again
It is raw, such as treatment burn, scald.
Water is solvent in the present invention；
BFGF acne hole maintenance freeze-dried powder lyase liquid is made by the component of following quality proportionings:
Human-like collagen: 1%-2%；
Collagen wins peptide: 0.5%-1%；
Dipropylene glycol: 3.8%-4.2%；
Propylene glycol: 1.8%-2.1%；
Glycerol: 1.8%-2.2%；
Sodium azulenesulfonate solution: 3%-5%；
GC-04:0.4-0.6%；
Water: 82.9%-87.7%；
Human-like collagen (Human-like Collagen) in the present invention is by biotechnology by human body glue
The mRNA reverse transcription of former albumen is at cDNA, and for one section of genetic recombination after digestion in E.coli (Escherichia coli), process is highly dense
Spend a kind of high-molecular biologic albumen of fermentation, separation, renaturation, purifying process production.Collagen is that people's in-vivo content is most
Protein is primarily present in skin, bone, cartilage, tendon, ligament and the blood vessel of human body, is of crucial importance in connective tissue
Structural protein, play support organ, protect the function of human body.It can be divided according to function and its design feature for constituting tissue
At: at fiber collagen, reticulate structure collagen, positioned at the fiber correlation collagen of fiber surface, it is former at beading silk gum, at flesh counterdie
Anchoring fiber collagen has transmembrane structure collagen etc..
Collagen in the present invention wins peptide (INCI: HYDROLYZED COLLAGEN/DIPEPTIDE-1, Chinese name
Claim: hydrolytic collagen/dipeptides -1), it is a kind of high molecular functional protein, it is the chief component of skin, and it is true to account for skin
The specific gravity of cortex 80%, it constitutes a fine and closely woven elasticated net in skin, securely locks moisture, and supporter's skin is rich in
The collagens characteristic amino acids such as glycine, proline, hydroxyl paving propylhomoserin that human body needs, are human body cell especially skin cells
Important component in epimatrix.This composite collagen victory peptide product has the following characteristics that the water conservation of 1, increase cuticula
Power enhances the tender degree of skin smooth；2, fine film can be formed on the skin, there is the effect of Firm at once；3, it directly permeates
To dermal layer of the skin, skin fibr tissue mechanism is reformed, promotes cell metabolism, delaying cell aging obtains fine wrinkles
To releiving；4, the water and oil balance for keeping skin, it is vigorous to avoid the occurrence of fat secretion；5, promote eye circumference to enclose skin microcirculation, reinforce
Eye skin metabolism improves eye pouch and black eye；6, stimulation cell division is proliferated, damaged cell is repaired in differentiation, healing wound
Mouthful；7, the injury of ultraviolet light (UVB) to skin is reduced.
Dipropylene glycol in the present invention has good moisture, and oil and hydrocarbon are total to molten ability, and smell is light
Micro-, to skin irritation very little, isomer is evenly distributed unanimously, and quality is splendid.
Propylene glycol in the present invention can be used as wetting agent with glycerol or sorbierite cooperation.
Glycerol in the present invention is colourless sweet clear and bright thick liquid.It is odorless.There is warm sweet taste.It can be used as solvent, lubricant,
Medicament and sweetener.
Sodium azulenesulfonate solution in the present invention is prepared by forming for following quality proportionings, sodium azulenesulfonate 0.45-
0.55% (No. CAS: 916445-22-2), axenic purification water 99.45-99.55%.Its effect has: 1, natural anti-inflammatory, anti-mistake
It is quick；2, aqueous easy absorption, sodium gualenate aqueous solution has inflammation caused by various control many kinds of substance, and effect is more lasting,
Inhibit inflammatory cell to release histamine by part directly effect, inhibits the synthesis of prostaglandin E2 in mucous membrane, granulation is promoted to be formed
With epithelial cell new life；3, ultraviolet light is effectively antagonized, the anti-UV activity index of sodium azulenesulfonate solution is higher, and water solubility is not added
The uvioresistant activity index of group is lower.
GC-04 extract in the present invention includes hydroxy acetophenone, glycerol caprylate and decoyl hydroximic acid, is a kind of natural
Plant extracts, can be described as no added preservative, and parahydroxyacet-ophenone is present in compositae plant artemisia scoparia (Artemisia
Scoparia Waldst.et kit.) stem, leaf and the anti-wormwood artemisia of mattress (A. capillaris Thunb.), Luo Mo section plant person
Join in the plants such as the root of radix tylophorae (Tylophora Kerri i Craib)；The pure natural glyceride type of glycerol caprylate is multi-functional
Alternative preservative is rapeseed oil and cocounut oil derivative, and the mild polarity of skin is good, and bacterium is inhibited to agree yeast increment activity,
There is moisturizing, emulsifies, moisturizing, wetting and Sage Extract and other effects；Decoyl hydroximic acid is a no added preservative, it can be killed
Bacterium is antibacterial, solubilized hydrotropy.
Water in the present invention is solvent.
The two of task of the present invention realize by following technical solution:
A kind of preparation method of above-mentioned bFGF acne hole maintenance freeze-dried powder preparation, the system including bFGF acne hole maintenance freeze-dried powder
The preparation method of Preparation Method and bFGF acne hole maintenance freeze-dried powder lyase liquid, wherein bFGF acne cheats the preparation method packet of maintenance freeze-dried powder
Include following steps:
S1: it the selection of raw material and prepares ratio: choosing the tap water by distillation and sterilization processing, mannitol,
BFGF, EGF are before carrying out mixing match in vacuum packaging, Sodium Hyaluronate constant temperature packaging；
S2: mannitol, the 0.05%-0.2% of bFGF, 5%-15% of 0.5%-1% are weighed by the quality proportioning
Sodium Hyaluronate, EGF, surplus water；
S3: bFGF, mannitol, Sodium Hyaluronate, EGF are placed in water, then mixture is placed in mixing plant
It is at the uniform velocity stirred, is to stir 10min in 600r/min in revolving speed, stirring forms mixed solution A；
S4: mixed solution A standing being again placed in mixing plant afterwards for 24 hours and is at the uniform velocity stirred, and is 1200r/ in revolving speed
20min is stirred in min；
S5: mixed solution A is filtered by filter membrane, is removed impurity not soluble in water in mixed solution A and is mixed
Close solution B；
S6: mixed solution B being placed in centrifugation apparatus and carries out centrifugally operated, then carries out precooling operation, at this time temperature
Control carries out refrigeration operation, mixed solution B lyophilization after the completion of -5 DEG C, precooling, and temperature is controlled at -15 DEG C.
S7: the mixed solution after lyophilization be placed in sterilizing vacuum bag be vacuum-packed, storage temperature control 0 DEG C with
Under；
Distillation tap water temperature in S1 is controlled at 50-70 DEG C；By the filter membrane diameter control in S5 in 0.1um by S6
Powder C need to be made in mixed liquor after lyophilization after ultraviolet sterilization；
Wherein bFGF acne hole maintenance freeze-dried powder lyase liquid preparation method the following steps are included:
S1, human-like collagen, collagen victory peptide stirring and dissolving to transparent, obtained material A are weighed by quality proportioning；
S2, the water stirring of material A and dipropylene glycol, propylene glycol, glycerol, sodium azulenesulfonate solution, GC-04, surplus is divided
It dissipates, is dispersed with stirring within the scope of 2.0-5.0Hz with dispersion machine to uniform, then through 150-250 mesh net filtration, bFGF is made
Acne cheats maintenance freeze-dried powder lyase liquid D, and powder C is mixed in bFGF acne hole maintenance freeze-dried powder lyase liquid D, obtains the hole maintenance of bFGF acne
Freeze-dried powder preparation.
Basic Fibroblast Growth Factor (abbreviation bFGF or oligopeptides -3) is that existing one kind is micro- in mammal and human body
BFGF receptor is distributed in quantity of material in Various Tissues cell, thus its physiological action is extensive, is a kind of multi-functional cell growth
The factor.BFGF effect extensively, to nearly all cell related with wound, such as fibroblast, vascular endothelial cell, smooth
Myocyte, epithelial cell, cartilage cell and nerve cell etc. have promotion proliferation and splitting action, to repair damaged cell and rush
The mode of aged cells is replaced into neoblast generation to achieve the effect that the profound bFGF of acne hole acne print on the market that repairs freezes
Dry powder can also repair acne and cheat acne print, but bFGF effect is 30-100 times of bFGF with anti-acne
BFGF acne hole maintenance freeze-dried powder preparation has following effect:
1, skin care, repair
BFGF can improve the microenvironment of cell growth, promote the synthesis of elastomer and collagen, keep skin rich
Elasticity makes skin be in sliding tender state.
2, crease-resistant, anti-aging effect
Can promote fibroblastic growth and development, aged cells are constantly replaced with new cell, thus generate it is wrinkle resistant,
Anti-wrinkle effect.
3, whitening, effect of dispelling spots
It updates senile cell and mitigates skin pigment to reduce the content of melanin and coloured corpuscle in Skin Cell
It is calm.
4, the effect of sun-proof and after-sun recovery
Damaged cell can be repaired rapidly, mitigated ultraviolet radiation and injured caused by skin.
5, anti-acne, go scar to act on
It stimulates the formation of skin granulation tissue and promotes the epithelialization of granulation tissue, can also adjust collagen degradation and update,
The effect formed so as to shorten wound healing time and reduction scar.
Water in the present embodiment is deionized water.
Above description is only embodiments herein and the explanation to institute's application technology principle.Those skilled in the art answer
Work as understanding, invention scope involved in the application, however it is not limited to technical solution made of the specific combination of above-mentioned technical characteristic,
It should also cover in the case where not departing from the inventive concept simultaneously, carry out any group by above-mentioned technical characteristic or its equivalent feature
The other technical solutions closed and formed.Such as features described above and (but being not limited to) disclosed herein have the skill of similar functions
Art feature is replaced mutually and the technical solution that is formed.
Claims (2)
1. a kind of bFGF acne cheats maintenance freeze-dried powder preparation, which is characterized in that including cheating maintenance freeze-dried powder and bFGF acne by bFGF acne
Cheat the solution that maintenance lyase liquid is constituted, wherein bFGF acne hole maintenance freeze-dried powder is made by the component of following quality proportionings:
Oligopeptides -3:0.5%-1%；
Mannitol: 5%-15%；
Sodium Hyaluronate: 0.02%-0.05%；
Oligopeptides -1:0.2%-0.5%；
Water: 83.45%-94.28%；
BFGF acne hole maintenance freeze-dried powder lyase liquid is made by the component of following quality proportionings:
Human-like collagen: 1%-2%；
Collagen wins peptide: 0.5%-1%；
Dipropylene glycol: 3.8%-4.2%；
Propylene glycol: 1.8%-2.1%；
Glycerol: 1.8%-2.2%；
Sodium azulenesulfonate solution: 3%-5%；
GC-04:0.4-0.6%；
Water: 82.9%-87.7%.
2. a kind of preparation method based on a kind of hole of bFGF acne described in claim 1 maintenance freeze-dried powder preparation, which is characterized in that packet
The preparation method of bFGF acne hole maintenance freeze-dried powder and the preparation method of bFGF acne hole maintenance freeze-dried powder lyase liquid are included, wherein bFGF acne
Cheat maintenance freeze-dried powder preparation method the following steps are included:
S1: the selection of raw material and prepare ratio: choosing the tap water by distillation and sterilization processing, mannitol, bFGF,
EGF is before carrying out mixing match in vacuum packaging, Sodium Hyaluronate constant temperature packaging；
S2: mannitol, the 0.05%-0.2% for weighing bFGF, 5%-15% of 0.5%-1% by the quality proportioning are transparent
Matter acid sodium, EGF, surplus water；
S3: bFGF, mannitol, Sodium Hyaluronate, EGF are placed in water, and then mixture is placed in mixing plant and is carried out
It at the uniform velocity stirs, is to stir 10min in 600r/min in revolving speed, stirring forms mixed solution A；
S4: mixed solution A standing being again placed in mixing plant afterwards for 24 hours and is at the uniform velocity stirred, and is in 1200r/min in revolving speed
Stir 20min；
S5: mixed solution A is filtered by filter membrane, and impurity not soluble in water obtains mixing molten in removing mixed solution A
Liquid B；
S6: mixed solution B being placed in centrifugation apparatus and carries out centrifugally operated, then carries out precooling operation, and temperature controls at this time
Refrigeration operation, mixed solution B lyophilization are carried out after the completion of -5 DEG C, precooling, temperature is controlled at -15 DEG C.
S7: the mixed solution after lyophilization is placed in sterilizing vacuum bag and is vacuum-packed, and storage temperature is controlled at 0 DEG C or less；
Distillation tap water temperature in S1 is controlled at 50-70 DEG C；S6 is distilled by the filter membrane diameter control in S5 in 0.1um and is done
Powder C need to be made in mixed liquor after dry after ultraviolet sterilization；
Wherein bFGF acne hole maintenance freeze-dried powder lyase liquid preparation method the following steps are included:
S1, human-like collagen, collagen victory peptide stirring and dissolving to transparent, obtained material A are weighed by quality proportioning；
S2, the water of material A and dipropylene glycol, propylene glycol, glycerol, sodium azulenesulfonate solution, GC-04, surplus is dispersed with stirring, is used
Dispersion machine is dispersed with stirring to uniform within the scope of 2.0-5.0Hz, then through 150-250 mesh net filtration, bFGF acne hole is made and repairs
Freeze-dried powder lyase liquid D is protected, powder C is mixed in bFGF acne hole maintenance freeze-dried powder lyase liquid D, obtains bFGF acne hole maintenance freeze-dried powder
Preparation.
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Cited By (4)
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| CN111166713A (en) * | 2019-12-23 | 2020-05-19 | 广州市暨源生物科技有限公司 | Freeze-dried powder with repairing activity and preparation method and application thereof |
| CN112516000A (en) * | 2020-12-25 | 2021-03-19 | 江苏江山聚源生物技术有限公司 | Repair stock solution containing recombinant collagen |
| CN113304063A (en) * | 2021-04-23 | 2021-08-27 | 四川省恩乐生物工程有限公司 | Composition for skin beautifying and anti-aging and preparation method thereof |
| CN116919849A (en) * | 2023-09-15 | 2023-10-24 | 中国科学院华南植物园 | Preparation method and application of transdermal collagen freeze-dried composition |
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| CN108498357A (en) * | 2018-04-13 | 2018-09-07 | 广州丽彦妆生物科技有限公司 | A kind of human-like collagen facial mask liquid and preparation method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111166713A (en) * | 2019-12-23 | 2020-05-19 | 广州市暨源生物科技有限公司 | Freeze-dried powder with repairing activity and preparation method and application thereof |
| CN112516000A (en) * | 2020-12-25 | 2021-03-19 | 江苏江山聚源生物技术有限公司 | Repair stock solution containing recombinant collagen |
| CN113304063A (en) * | 2021-04-23 | 2021-08-27 | 四川省恩乐生物工程有限公司 | Composition for skin beautifying and anti-aging and preparation method thereof |
| CN116919849A (en) * | 2023-09-15 | 2023-10-24 | 中国科学院华南植物园 | Preparation method and application of transdermal collagen freeze-dried composition |
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Application publication date: 20190308 |