CN109429618A - A method of improving corn germination rate - Google Patents

A method of improving corn germination rate Download PDF

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Publication number
CN109429618A
CN109429618A CN201811529295.6A CN201811529295A CN109429618A CN 109429618 A CN109429618 A CN 109429618A CN 201811529295 A CN201811529295 A CN 201811529295A CN 109429618 A CN109429618 A CN 109429618A
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Prior art keywords
seed
corn
germination
germination rate
improving
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Inventor
米铁柱
刘日辉
沈妍
罗碧
王克响
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Yuanmi Agricultural Science And Technology Co Ltd
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Yuanmi Agricultural Science And Technology Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/02Germinating apparatus; Determining germination capacity of seeds or the like
    • A01C1/025Testing seeds for determining their viability or germination capacity
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/08Immunising seed

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  • Life Sciences & Earth Sciences (AREA)
  • Soil Sciences (AREA)
  • Environmental Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physiology (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

This application discloses a kind of methods for improving corn germination rate, comprising the following steps: (1) bask seeds: fine warm weather teds seed 3~5 days;(2) it soaks seed: seed is dipped in diluted formalin solution, covering keeps moist after taking-up, then is rinsed twice with clear water, pulls out, dries in the shade;(3) it sterilizes: after corn seed is cleaned, being put into alcohol and impregnate, be then placed in 0.1% mercuric chloride and impregnate, then use sterile water wash;(4) vernalization: corn seed being uniformly placed in the germination box for being covered with germination paper, and water is added and impregnates, and wet vernalization is kept under the conditions of 20~25 DEG C are put into after immersion.Using the method for the present invention, the germination percentage of corn seed ensure that not less than 96%, seedlings of cereal crops root system item number is more, root hair is more, root volume is big, and sprout term disease does not occur, and standing grain packing is good, not slow seedling of after planting turning green.

Description

A method of improving corn germination rate
Technical field
The present invention relates to modern agricultural technology field of agricultural cultivation, in particular to a kind of method for improving corn germination rate.
Background technique
Corn is annual monoecism cross-pollinatd plant, and plant is tall and big, and stem is strong, is important cereal crops and feeding Material crop and the highest crops of whole world total output, cultivated area and total output are only second to corn and wheat.Corn one Directly all it is known as life prolonging food, protein rich in, fat, vitamin, microelement, cellulose etc. have exploitation high Nutrition, high biological function food great potential.But since its heredity is complex, variation type is abundant, in routine In breeding process there is excessive cycle, the coefficient of variation is excessive, influences the shortcomings that offspring development, and modern biotechnology breeding skill Art not only overcomes disadvantages mentioned above and deficiency, while also improving breeding speed and quality.
Corn worldwide has extensive cultivated area, total output exists as a kind of important industrial crops Occupy very big ratio in whole cereal crops, is indispensable cereal crop in people's life.China is maize seed Big country is planted, corn plays a crucial role in national life, mentions in the period of China is underdeveloped for China people Basic food security is supplied.Along with the rapid development of Chinese national economy, living standards of the people are continuously improved, domestic market The demand of corn is also increasing year by year.
Corn is one of most important grain in the world, and there are about one third populations using corn as main for world today Grain, wherein corn accounts for 50%, Duo Zheda 90% so that Africa accounts for 25%, and Latin America accounts for 40% in Asian food composition. The protein content of corn is higher than rice, and fat content is higher than flour, rice and millet, and heat content is higher than flour, rice and height Fine strain of millet, but the disadvantage is that particle is big, poor eating, sticky school in the lower area of living standard, corn is important grain.City and compared with Developed regions, corn are the adjustment indispensable food of taste.With the development of Processing Industry of Con Grain, the edible quality of corn is not Disconnected to improve, new corn food such as cornflakes, maize flour, maize pulp, special corn flour, fast food corn etc. generates therewith, and can Noodles, bread, biscuit etc. is further made.Corn also can be processed into as zein, corn oil, monosodium glutamate, soy sauce, white wine etc., These products are at home and abroad popular in the market.
Corn is the king of feed.It is reported that the feeding value of double centner corn is equivalent to 135 kilograms of oats, 120 kilograms Sorghum or 150 kilograms of long-grained nonglutinous rices.With corn feed as main component, every 2~3 kilograms can gain 1 kilogram of meat, the pair of corn Product stalk may be made as ensilage.The corn of about 65-70% is used as feed in the world, and developed country is up to 80%, It is that animal husbandry is rely the important foundation of development.
Maize kernel is important the raw material of industry, and preliminary working and deep processing can produce two, 300 kind of product.Preliminary working product It can be used as basic material further processing with byproduct to utilize, it is raw in the industry such as food, chemical industry, fermentation, medicine, weaving, papermaking Miscellaneous product is manufactured in production, cob can produce furfural.In addition, corn stover and cob can cultivate production edible mushroom, Bract can weave hand basket, carpet, sit the artifacts such as blanket, and marketing is domestic and international.
Currently, corn is no longer only used for meeting the problem of food and clothing of people and the feed problem of full feeding domestic animal, and It is more for meeting people to healthier quality of the life and richer dietetic variety, thus corn is planted in its people It is still occupied an important position in economy.The maize sown area in China was also constantly expanding in recent years, including extensive The small-scale family planting mode in corn planting base and rural area, no matter which kind of cropping pattern, the germination of corn seed is blunt It connects and limits the later period to the effect of the work such as fertilising, the watering of corn, also directly determine the yield and quality of corn.
With the increasingly raising of people's living standard, the requirement to diet nutritional is also being continuously increased, so that disappearing to corn The quantity of expense is also increasing.The germination percentage of corn seed has important influence to the yield and quality of corn.Corn seed Germination percentage is also the important indicator of corn seed quality height simultaneously, and percentage of seedgermination is low, and to will cause after planting seed uneven, young Seedling growing way is bad, eventually leads to corn yield decline.
In the agricultural technology extension practice of many years, the advanced technology of many corn plantings, corn have successively been promoted and applied Yield be also continuously improved.Some problems are still had in maize production, affect the stability of corn yield.Corn seed Germination percentage receive the influences of the factors such as temperature, humidity and soil nutrient, and the upgrowth situation and corn of corn seedling whether It can be realized the height that high yield directly depends on corn seed germination rate.
Summary of the invention
Technical problem to be solved by the present invention lies in provide a kind of method for improving corn germination rate.The method of the present invention, Corn seed germination rate can be greatly improved, solves the problems, such as corn breeding, method is easy, and it is at low cost, it is profitable.
In order to solve the above technical problems, the present invention provides a kind of methods for improving corn germination rate, comprising the following steps:
(1) bask seeds: fine warm weather teds seed 3~5 days;
(2) it soaks seed: seed is dipped in diluted formalin solution, covering keeps moist after taking-up, then is rushed with clear water It washes twice, pulls out, dry in the shade;
(3) it sterilizes: after corn seed is cleaned, being put into alcohol and impregnate, be then placed in 0.1% mercuric chloride and impregnate, then use Sterile water wash;
(4) vernalization: corn seed being uniformly placed in the germination box for being covered with germination paper, and water is added and impregnates, after immersion Wet vernalization is kept under the conditions of being put into 20~25 DEG C.
Preferably, in the step (2), seed is dipped in 25- in the formalin solution of 200-300 times of liquid 40% of dilution 30 minutes.
Preferably, it in the step (2), takes out covering and is kept for humidity 1.5-2.5 hours.
Preferably, it in the step (3), after corn seed is cleaned, is put into 75% alcohol and impregnates 20-40s, then put Enter in 0.1% mercuric chloride and impregnates 5-9min.
Preferably, in the step (4), corn seed is uniformly placed in the germination box for being covered with germination paper, is added 30~50 DEG C of distilled water immersions.
Preferably, in the step (4), large seed impregnates 7~9h.
Preferably, in the step (4), seed impregnate after be placed in 5 DEG C of constant temperature illumination boxs handle 10d move back to In 22-26 DEG C of constant temperature illumination box.
In order to solve the above technical problems, the present invention also provides a kind of measuring method of corn germination rate, including following step It is rapid:
(1) bask seeds: fine warm weather teds seed 3~5 days;
(2) it soaks seed: seed is dipped in diluted formalin solution, covering keeps moist after taking-up, then is rushed with clear water It washes twice, pulls out, dry in the shade;
(3) it sterilizes: after corn seed is cleaned, being put into alcohol and impregnate, be then placed in 0.1% mercuric chloride and impregnate, then use Sterile water wash;
(4) vernalization: corn seed being uniformly placed in the germination box for being covered with germination paper, and water is added and impregnates, seed leaching It is placed on processing 10d in 5 DEG C of constant temperature illumination boxs after bubble to move back into 25 DEG C of constant temperature illumination boxs, kind is measured after 7d Sub- germination percentage.
In order to solve the above technical problems, the present invention also provides such as any one of aforementioned methods for improving corn germination rate in jade Application in rice plantation.
In order to solve the above technical problems, the present invention also provides such as any one of aforementioned methods for improving corn germination rate to mention Application in high corn yield.
The beneficial effects of the invention are that: compared with prior art, present invention mainly solves corn germination rate asking lowly Topic, the method for the raising corn seed germination rate provided, improves emphatically the germination percentage of corn seed, using the method for the present invention, The germination percentage of corn seed be ensure that not less than 96%, seedlings of cereal crops root system item number is more, root hair is more, root volume is big, and seedling stage disease does not occur Evil, standing grain packing is good, not slow seedling of after planting turning green, and directly takes root and survives, it can be achieved that increasing both production and income, effectively reduces seed costs, Have great importance to the safety kind for guaranteeing agricultural production.
Specific embodiment
The present invention is described in detail below with reference to embodiment.To keep the objectives, technical solutions, and advantages of the present invention clearer, bright Really, the present invention is described in more detail below, but the invention is not limited to these embodiments.
The method that the present invention improves corn germination rate, steps are as follows:
(1) bask seeds: fine warm weather teds seed 3~5 days;
(2) it soaks seed: seed is dipped in the formalin solution of 200-300 times of liquid 40% of dilution 25-30 minutes, take out kind Seed is covered and is allowed to keep 1.5-2.5 hours moist with wet gauze after son, then is rinsed twice with clear water, is pulled out, yin It is dry;
(3) it sterilizes: after corn seed is cleaned, being put into 75% alcohol and impregnate 20-40s, be then placed in 0.1% mercuric chloride 5-9min is impregnated, then with sterile water wash 5 times;
(4) vernalization: corn seed being uniformly placed in the germination box for being covered with germination paper, and 30~50 DEG C of distilled water are added It impregnating, small-sized seed (>=2000/L) (maize kernel size (grain/L)=bulk density (g/L)/100-grain weight x100) impregnates 5~6h, (≤1700/L) 7~9h of immersion of large seed, seed keep wet vernalization under the conditions of being put into 20~25 DEG C after impregnating.
Preferably, steps are as follows:
(4) vernalization: corn seed being uniformly placed in the germination box for being covered with germination paper, and 30~50 DEG C of distilled water are added Immersion 5~6h the large seed (≤1700/L) of small-sized seed (>=2000/L) 7~9h of immersion is impregnated, seed is placed after impregnating 10d is handled in 5 DEG C of constant temperature illumination boxs to move back into 25 DEG C of constant temperature illumination boxs, and germination is measured after 7d Rate.
The present invention also provides a kind of measuring methods of corn germination rate, comprising the following steps:
(1) bask seeds: fine warm weather teds seed 3~5 days;
(2) it soaks seed: seed is dipped in diluted formalin solution, covering keeps moist after taking-up, then is rushed with clear water It washes twice, pulls out, dry in the shade;
(3) it sterilizes: after corn seed is cleaned, being put into alcohol and impregnate, be then placed in 0.1% mercuric chloride and impregnate, then use Sterile water wash;
(4) vernalization: corn seed being uniformly placed in the germination box for being covered with germination paper, and water is added and impregnates, seed leaching It is placed on processing 10d in 5 DEG C of constant temperature illumination boxs after bubble to move back into 25 DEG C of constant temperature illumination boxs, kind is measured after 7d Sub- germination percentage.
The present invention also provides application of such as any one of aforementioned method for improving corn germination rate in corn planting.
The present invention also provides such as any one of aforementioned methods for improving corn germination rate to improve the application in corn yield.
Embodiment 1:
1, it soaks seed.
(1) bask seeds: fine warm weather teds seed 3~5 days.Can sterilizing disease prevention, intensify seed enzymatic activity, also advantageous kind Son water suction;
(2) seed soaking step: seed being dipped in the formalin solution of 200 times of liquid 40% of dilution, about 25-30 minutes, Seed is covered with wet gauze after taking-up and keeps 2 hours moist, then is rinsed twice with clear water;After corn seed is cleaned, It is put into 75% alcohol and impregnates 30s, be then placed in 0.1% mercuric chloride and impregnate 7min, then with sterile water wash 5 times;
2, soaking corn seed
Experimental group A: 300 same kind corn seeds are impregnated in the formalin solution for diluting 200 times of liquid 40%, About 30 minutes, then rinsed twice with clear water;After corn seed is cleaned, it is put into 75% alcohol and impregnates 30s, be then placed in 7min is impregnated in 0.1% mercuric chloride, then with sterile water wash 5 times;
Control group A: by 300 same kind corn seeds 30 minutes in clear water;
300 corn seeds are taken, are uniformly placed in the germination box for being covered with germination paper, appropriate distilled water is added.Setting 3 A repetition is placed on processing 10d in 5 DEG C of constant temperature illumination boxs and moves back into 25 DEG C of constant temperature illumination boxs, surveyed after 7d Determine percentage of seedgermination.As shown in table 1 below, existed using the corn seed germination rate that formalin solution seed soaking and alcohol disinfecting are crossed 95% or more, and control group corn seed germination rate is up to just to 79%.
1 experimental group of table and control group corn germination rate situation
Embodiment 2:
1, it soaks seed.
(1) bask seeds: fine warm weather teds seed 3~5 days.Can sterilizing disease prevention, intensify seed enzymatic activity, also advantageous kind Son water suction;
(2) seed soaking step: seed being dipped in the formalin solution of 250 times of liquid 40% of dilution, about 25-30 minutes, Seed is covered with wet gauze after taking-up and keeps 2 hours moist, then is rinsed twice with clear water;After corn seed is cleaned, It is put into 75% alcohol and impregnates 30s, be then placed in 0.1% mercuric chloride and impregnate 7min, then with sterile water wash 5 times;
2, soaking corn seed
Experimental group B: 300 same kind corn seeds are impregnated in the formalin solution for diluting 250 times of liquid 40%, About 30 minutes, then rinsed twice with clear water;After corn seed is cleaned, it is put into 75% alcohol and impregnates 30s, be then placed in 7min is impregnated in 0.1% mercuric chloride, then with sterile water wash 5 times;
Control group B: by 300 same kind corn seeds 30 minutes in clear water;
300 corn seeds are taken, are uniformly placed in the germination box for being covered with germination paper, appropriate distilled water is added.Setting 3 A repetition is placed on processing 10d in 5 DEG C of constant temperature illumination boxs and moves back into 25 DEG C of constant temperature illumination boxs, surveyed after 7d Determine percentage of seedgermination.As shown in table 2 below, existed using the corn seed germination rate that formalin solution seed soaking and alcohol disinfecting are crossed 96.7% or more, and control group corn seed germination rate is up to just to 80%.
Corn seed germination rate situation in 2 experimental group of table and control group
Embodiment 3:
1, it soaks seed.
(1) bask seeds: fine warm weather teds seed 3~5 days.Can sterilizing disease prevention, intensify seed enzymatic activity, also advantageous kind Son water suction;
(2) seed soaking step: seed being dipped in the formalin solution of 300 times of liquid 40% of dilution, about 25-30 minutes, Seed is covered with wet gauze after taking-up and keeps 2 hours moist, then is rinsed twice with clear water;After corn seed is cleaned, It is put into 75% alcohol and impregnates 30s, be then placed in 0.1% mercuric chloride and impregnate 7min, then with sterile water wash 5 times;
2, soaking corn seed
Experimental group C: 300 same kind corn seeds are impregnated in the formalin solution for diluting 300 times of liquid 40%, About 30 minutes, then rinsed twice with clear water;After corn seed is cleaned, it is put into 75% alcohol and impregnates 30s, be then placed in 7min is impregnated in 0.1% mercuric chloride, then with sterile water wash 5 times;
Control group C: by 300 same kind corn seeds 30 minutes in clear water;
300 corn seeds are taken, are uniformly placed in the germination box for being covered with germination paper, appropriate distilled water is added.Setting 3 A repetition is placed on processing 10d in 5 DEG C of constant temperature illumination boxs and moves back into 25 DEG C of constant temperature illumination boxs, surveyed after 7d Determine percentage of seedgermination.As shown in table 2 below, existed using the corn seed germination rate that formalin solution seed soaking and alcohol disinfecting are crossed 98% or more, and control group corn seed germination rate is up to just to 80%,
Corn seed germination rate situation in 3 experimental group of table and control group
To sum up the result shows that the germination percentage of experimental group corn seed is 95% or more, and in control group corn seed hair Bud rate is then hovered 80% or so, and the corn seed crossed with alcohol disinfecting is impregnated using formalin solution, and germination percentage compares According to a group height.For the average corn germination rate of experimental group A up to 96%, the average germination percentage of experimental group B is real up to 97% in three experimental groups The average corn germination rate of group C is tested up to 98.8%, is found in conjunction with each group of data, the combination in embodiment 3 can reach preferable effect Fruit.Therefore the invention can be improved the germination percentage of corn to reduce the economic loss of peasant household.
The above is only several embodiments of the present invention, not any type of limitation is done to the present invention, although this hair It is bright to be disclosed as above with preferred embodiment, however be not intended to limit the invention, any person skilled in the art, it is not taking off In the range of technical solution of the present invention, a little variation or modification are made using the technology contents of the disclosure above and is equal to Case study on implementation is imitated, is belonged in technical solution of the present invention protection scope.

Claims (10)

1. a kind of method for improving corn germination rate, which comprises the following steps:
(1) bask seeds: fine warm weather teds seed 3~5 days;
(2) it soaks seed: seed is dipped in diluted formalin solution, seed is covered and kept with wet gauze after taking-up Humidity, then rinsed twice with clear water, it pulls out, dries in the shade;
(3) it sterilizes: after corn seed is cleaned, being put into alcohol and impregnate, be then placed in 0.1% mercuric chloride and impregnate, then with sterile Water cleaning;
(4) vernalization: corn seed being uniformly placed in the germination box for being covered with germination paper, and water is added and impregnates, is put into after immersion Wet vernalization is kept under the conditions of 20~25 DEG C.
2. improving the method for corn germination rate according to claim 1, which is characterized in that in the step (2), seed is soaked 25-30 minutes in the formalin solution for diluting 200-300 times of liquid 40%.
3. improving the method for corn germination rate according to claim 1, which is characterized in that in the step (2), take out seed It is covered with wet gauze, and keeps 1.5-2.5 hours moist.
4. improving the method for corn germination rate according to claim 1, which is characterized in that in the step (3), by maize seed After son is cleaned, it is put into 75% alcohol and impregnates 20-40s, be then placed in 0.1% mercuric chloride and impregnate 5-9min.
5. improving the method for corn germination rate according to claim 1, which is characterized in that in the step (4), by maize seed Son is uniformly placed in the germination box for being covered with germination paper, and 30~50 DEG C of distilled water immersions are added.
6. improving the method for corn germination rate according to claim 1, which is characterized in that in the step (4), small-sized seed 5~6h is impregnated, large seed impregnates 7~9h.
7. improving the method for corn germination rate according to claim 1, which is characterized in that in the step (4), seed impregnates After be placed in 5 DEG C of constant temperature illumination boxs handle 10d move back into 22-26 DEG C of constant temperature illumination box.
8. a kind of measuring method of corn germination rate, which comprises the following steps:
(1) bask seeds: fine warm weather teds seed 3~5 days;
(2) it soaks seed: seed is dipped in diluted formalin solution, seed is covered and is allowed to wet gauze after taking-up It keeps moist, then rinsed twice with clear water, pulls out, dry in the shade;
(3) it sterilizes: after corn seed is cleaned, being put into alcohol and impregnate, be then placed in 0.1% mercuric chloride and impregnate, then with sterile Water cleaning;
(4) vernalization: corn seed being uniformly placed in the germination box for being covered with germination paper, and water is added and impregnates, after seed impregnates It is placed on processing 10d in 5 DEG C of constant temperature illumination boxs to move back into 25 DEG C of constant temperature illumination boxs, seed hair is measured after 7d Bud rate.
9. as any one of claim 1-7 improves application of the method for corn germination rate in corn planting.
10. as any one of claim 1-7 method for improving corn germination rate is improving the application in corn yield.
CN201811529295.6A 2018-12-13 2018-12-13 A method of improving corn germination rate Pending CN109429618A (en)

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Publication number Priority date Publication date Assignee Title
CN111630973A (en) * 2020-05-26 2020-09-08 湖南碧田园生态农业科技有限公司 Crop seed germination method for 6 hours
CN112534988A (en) * 2020-12-28 2021-03-23 湖南省棉花科学研究所 Corn breeding method for improving germination rate
CN114303515A (en) * 2021-12-23 2022-04-12 昆明学院 Method for removing endophytes from corn seeds and rapidly germinating corn seeds

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CN106977246A (en) * 2017-03-02 2017-07-25 安徽金培因科技有限公司 A kind of efficient germination accelerating method of organic waxy corn
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111630973A (en) * 2020-05-26 2020-09-08 湖南碧田园生态农业科技有限公司 Crop seed germination method for 6 hours
CN112534988A (en) * 2020-12-28 2021-03-23 湖南省棉花科学研究所 Corn breeding method for improving germination rate
CN114303515A (en) * 2021-12-23 2022-04-12 昆明学院 Method for removing endophytes from corn seeds and rapidly germinating corn seeds

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Application publication date: 20190308