CN109418003A - The culture medium prescription and its cultural method of Antrodia camphorata - Google Patents
The culture medium prescription and its cultural method of Antrodia camphorata Download PDFInfo
- Publication number
- CN109418003A CN109418003A CN201710544301.4A CN201710544301A CN109418003A CN 109418003 A CN109418003 A CN 109418003A CN 201710544301 A CN201710544301 A CN 201710544301A CN 109418003 A CN109418003 A CN 109418003A
- Authority
- CN
- China
- Prior art keywords
- antrodia camphorata
- culture medium
- liquid
- timber
- bacterium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Mycology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Mushroom Cultivation (AREA)
Abstract
The present invention provides the culture medium prescription and its cultural method of a kind of Antrodia camphorata, the method includes obtaining original seed, prepare liquid spawn, prepare culture medium for cultivating, inoculation, bacterium germination, fruit body development management and etc., when preparing culture medium for cultivating, diameter 20cm is made in toothed oak ebon, after the timber of long 20cm, it puts and is totally submerged immersion 28 days in water, lignin is added in soaking process, glucose, corn flour %, the nutriments such as potassium dihydrogen phosphate, increase the nutriment and microelement on timber surface layer in soaking process, improve PH, conducive to mycelia vegetative growth phase fast-growth in the early stage, reduce the infection of miscellaneous bacteria.
Description
Technical field
The present invention relates to the culture medium prescriptions and its cultivation of edible and medical fungi production technical field more particularly to a kind of Antrodia camphorata
Method.
Background technique
Antrodia camphorata belongs to Aphyllophorales, Polyporaceae, antrodia karst, perennial gill fungus mushroom, is nineteen ninety just by biochemical boundary
The novel species delivered.Natural Antrodia camphorata is because being only grow on the hollow treehole and its section of wood of Taiwan endemic tree Cinnamomum kanahirai hay veteran, and mesh
Only Taiwan is more for preceding cinnamomum kanehirai, and belongs to world first class of protection tree species, the very rare price of wild resource and its valuableness, uses
It is not future thrust that Cinnamomum kanahirai hay wood, which produces Antrodia camphorata,.It gains the name because being grown in the peculiar protection class plant cinnamomum kanehirai in Taiwan, is
Precious rare medicinal fungi.Antrodia camphorata fructification is being that Taiwan indigenous peoples is used to treat the liver being led to by insobriety in early days
Damage, the jewellery be still drank after a night of solution are widely used in liver protecting and anti-oxidant, anti-inflammatory, antitumor etc. now.
Summary of the invention
In view of the deficiencies of the prior art, the present invention provides the culture medium prescription and its cultural method of a kind of Antrodia camphorata,
Realize the volume production cultivation of Antrodia camphorata fructification.
The present invention is achieved through the following technical solutions, provides culture medium prescription and its cultivation side of a kind of Antrodia camphorata
Method, described method includes following steps:
S01: strain is obtained: purchase Antrodia camphorata strain, and be stored on PDA slant medium.
S02: preparation liquid spawn: 150ml liquid seed culture medium is added into the triangular flask of 500ml, is then accessed
The strain obtained in the step S01, rotary shaker, revolving speed 160r/min are trained under 22~28 DEG C of temperature of dark condition
It supports 8~16 days, obtains liquid Antrodia camphorata strain.
S02: it prepares culture medium for cultivating: diameter 20cm is made in toothed oak ebon, the timber of long 20cm is put and is totally submerged in water
It impregnates 28 days, lignin 3%, glucose 2%, corn flour 1%, potassium dihydrogen phosphate 1.5% is added in soaking process；It will after the completion of impregnating
Bark, which is cleaned out, to be fitted into polypropylene 500*500 bacterium bag, and bacterium bag mouth is locked with the plastic tube of diameter 10cm, then with several layers of nothing
Woven fabric is sealed after packing tightly plastic tube nozzle and is tightened, and is placed in progress sterilization 12h under high-temperature and high-pressure conditions, is then being purified
It is cooling in workshop.
When timber impregnates in water, although being sufficiently humidified so as to, the nutriment in timber has certain loss, the present invention
The nutriments such as lignin are added in soaking process, increase the nutriment and microelement on timber surface layer, are improved PH, are conducive to
Mycelia vegetative growth phase fast-growth in the early stage, reduces infecting for miscellaneous bacteria.Relative to not adding nutriment, when mycelia grows
Between shorten or so two weeks.Plastic bushing can be made into the straight of different size according to air environment needed for Antrodia camphorata growth in bacterium bag
Diameter, 7 layers of non-woven fabrics are enough to obstruct miscellaneous bacteria in air again to be entered bacterium bag and pollutes, and this mouth-sealing method makes empty with the external world in bacterium bag
Gas is kept unimpeded, and ensure that the humidity of oxygen supply and the growth of suitable mycelia sufficient in mycelia growth course；Be conducive to mycelia
Development.
S03: inoculation: on the superclean bench of cleaning shop, the liquid spawn fermented is shaken up, the bacterium that will have been cooled down
Bag is opened, and is accessed 120~200ml liquid Antrodia camphorata strain with liquid inoculation rifle, is made Antrodia camphorata liquid spawn can be from toothed oak wood timber
Each section of surface current under, then bacterium bag tightened.
S04: bacterium germination: being placed on temperature control for inoculated bacterium bag and cultivate in 18~30 DEG C of cleaning shop, mycelia
On toothed oak wood timber surface, covering covers with and gos deep into wooden growth inside.
S05: fruit body development management: the good polypropylene 500*500 bacterium bag for installing timber of bacterium germination is taken off into bag, sending out
The toothed oak wood timber of bacterium is placed in humidity 75~95%, 16~28 DEG C of temperature of cleaning shop is cultivated, without light requirement in incubation
According to.
The method that this law provides is carried out in cleaning shop, and the large-scale planting of Antrodia camphorata fructification may be implemented.
Preferably, the liquid spawn fermented is the liquid ox that will be obtained in the step S02 in the step S03
In antrodia strain access sterilizing and fermentor one time fermentation culture medium after cooling, 22~28 DEG C of constant temperature are kept in fermentor,
It is passed through filtrated air culture 6~12 days and obtains.
Preferably, the fermentor one time fermentation culture medium includes the component of following mass fraction: wheat bran: 5%, corn
Powder: 3%, wheat powder: 8%, glucose: 1.5%, KH2P04: 0.1%, MgS04: 0.15%, peptone: 0.2 %, surplus are water.
Corn flour is both nitrogen source and carbon source, provides more comprehensive nutrition for culture medium.Magnesium sulfate can excite enzyme activity
Property, improve mycelia vigor.Peptone meets the needs of strain fast-germination as quick-acting nitrogen sources.Potassium dihydrogen phosphate provides micro
Element, and play the role of buffering pH value.
Preferably, the liquid seed culture medium includes: carrot 200g, glucose 20g, water in the step S02
1000ml。
Technical solution provided in an embodiment of the present invention may include it is following the utility model has the advantages that
(1) at present in ground for the research and development of Antrodia camphorata and using still in its infancy, in addition to a small number of liquid-state fermentation technologies
Except successful research report, so far there is not yet the large-scale planting of fructification is successfully reported.The present invention directly will inoculation, hair
The key operations such as bacterium, fructification management carry out in the environment of cleaning shop, get rid of the environment of laboratory constant temperature and humidity,
Cleaning shop has obtained large batch of test related ends, lays a good foundation for Antrodia camphorata fructification large-scale planting.
(2) it is greatly shortened with the cultivation cycle of toothed oak wood cultivation Antrodia camphorata fructification compared with the cultivation cycle of Cinnamomum kanahirai hay wood, Taiwan is wild
Substantially 2~3 years raw Cinnamomum kanahirai hay wood Antrodia camphorata growth period, and the present invention cultivates the growth period of Antrodia camphorata fructification with toothed oak wood as 1
~1.5 years.Growth cycle compared to wild Antrodia camphorata can so be greatly improved, and have benefited from the environment control of cleaning shop
System is suitable for the growth of Antrodia camphorata.In Antrodia camphorata strain bacterium germination stage cleaning shop temperature control between 18~30 DEG C, in fructification
The development growth stage, cleaning shop temperature control was between 16~28 DEG C.Fully understand the physiological property of Antrodia camphorata, scientific and reasonable tune
The most suitable growth environment needed for whole Antrodia camphorata growth and development different phase.
(3) in the cultivation base preparation process of toothed oak wood cultivation Antrodia camphorata fructification, the present invention is used bacterium bag mouth diameter
The plastic tube of 10cm is locked, then seals and tighten after being packed tightly plastic tube nozzle with several layers of non-woven fabrics.The diameter that the present invention uses
10cm plastic tube can be made into the diameter of different size according to air environment needed for Antrodia camphorata growth in bacterium bag, and several layers of non-woven fabrics are again
It is enough to obstruct miscellaneous bacteria in air and enters bacterium bag and pollute, this mouth-sealing method makes to keep unimpeded in bacterium bag with outside air, guarantor
The humidity of oxygen supply and the growth of suitable mycelia sufficient in mycelia growth course is demonstrate,proved.Toothed oak wood will be installed using set ring taps in the past
The polypropylene 500*500 bacterium bag mouth of dress seals, and set ring taps seals so that air and the external world in bacterium bag are completely isolated, hyphal development
Later period can be due to bag internal oxygen unsaturated vapor, and gas concentration lwevel is excessively high and stops growing.
(4) the original host's cinnamomum kanehirai of wild Antrodia camphorata is to protect kind, scarcity of resources, price extremely expensive, and the present invention adopts
The Antrodia camphorata cultivated with toothed oak wood is substantially close with the Antrodia camphorata that Cinnamomum kanahirai hay wood is cultivated on morphological appearance and in triterpenes content.
Simultaneously compared to other timber, toothed oak wood but also with not vulnerable to miscellaneous bacteria infection, do not recruit the characteristic of insect pest, more crucially toothed oak wood is free of
Pesticide residue effectively reduces pollution rate, improves the yield rate and the factor of merit of product.
Specific embodiment
Technical solution in order to enable those skilled in the art to better understand the present invention below will be implemented the present invention
Technical solution in example is clearly and completely described, it is clear that described embodiment is only that present invention a part is implemented
Example, instead of all the embodiments.Based on the embodiments of the present invention, those skilled in the art are not making creative work
Under the premise of every other embodiment obtained, protection scope of the present invention all should belong to.
Embodiment 1
The present embodiment provides the culture medium prescription and its cultural method of a kind of Antrodia camphorata, the scheme in accordance with the following steps into
Row:
S01: strain is obtained: purchase Antrodia camphorata strain, and be stored on PDA slant medium；
S02: preparation liquid spawn: 150ml liquid seed culture medium is added into the triangular flask of 500ml, then access activation
Antrodia camphorata strain, rotary shaker, revolving speed 160r/min cultivates 10 days under 23 DEG C of temperature of dark condition, obtains liquid
Antrodia camphorata strain；
S03: it prepares culture medium for cultivating: diameter 20cm is made in toothed oak ebon, the timber of length 20cm is put and is totally submerged leaching in water
Lignin, glucose, corn flour and potassium dihydrogen phosphate is added in bubble 30 days in soaking process, clear up bark after the completion of immersion and do
Only it is fitted into polypropylene 500*500 bacterium bag, bacterium bag mouth is locked with the plastic tube of diameter 10cm, then with 7 layers of non-woven fabrics by plastics
Pipe nozzle is sealed after packing tightly and is tightened, and is placed in 105 DEG C of autoclave sterilization tank after carrying out 12h sterilization in cleaning shop
Interior cooling；
S04: inoculation: on the superclean bench of cleaning shop, the liquid spawn fermented is shaken up, the bacterium bag cooled down is beaten
It opens, the Antrodia camphorata liquid spawn of 200ml or so is accessed with liquid inoculation rifle, make Antrodia camphorata liquid spawn can be from each of toothed oak wood timber
Under a section of surface current, then bacterium bag tightened；
S05: bacterium germination: being placed on temperature control for inoculated bacterium bag and cultivate in 20 DEG C of cleaning shop, and mycelia is in toothed oak wood wood
The covering of stake surface covers with and gos deep into wooden growth inside；
S06: fruit body development management: taking off bag for the good polypropylene 500*500 bacterium bag for installing timber of bacterium germination, sending out bacterium good
Toothed oak wood timber is placed in humidity 70%, 15 DEG C of temperature of cleaning shop is cultivated, until Antrodia camphorata fructification is long is to 1.5~3cm
It can pick, illumination is not necessarily in incubation.
Embodiment 2
The present embodiment provides the culture medium prescription and its cultural method of a kind of Antrodia camphorata, the scheme in accordance with the following steps into
Row:
S01: strain is obtained: purchase Antrodia camphorata strain, and be stored on PDA slant medium；
S02: preparation liquid spawn: 150ml liquid seed culture medium is added into the triangular flask of 500ml, then access activation
Antrodia camphorata strain, rotary shaker, revolving speed 160r/min cultivates 15 days under 27 DEG C of temperature of dark condition, obtains liquid
Antrodia camphorata strain；
S03: it prepares culture medium for cultivating: diameter 20cm is made in toothed oak ebon, the timber of length 20cm is put and is totally submerged leaching in water
Lignin, glucose, corn flour and potassium dihydrogen phosphate is added in bubble 30 days in soaking process, clear up bark after the completion of immersion and do
Only it is fitted into polypropylene 500*500 bacterium bag, bacterium bag mouth is locked with the plastic tube of diameter 10cm, then with 7 layers of non-woven fabrics by plastics
Pipe nozzle is sealed after packing tightly and is tightened, and is placed in 105 DEG C of autoclave sterilization tank after carrying out 12h sterilization in cleaning shop
Interior cooling；
S04: inoculation: on the superclean bench of cleaning shop, the liquid spawn fermented is shaken up, the bacterium bag cooled down is beaten
It opens, the Antrodia camphorata liquid spawn of 200ml or so is accessed with liquid inoculation rifle, make Antrodia camphorata liquid spawn can be from each of toothed oak wood timber
Under a section of surface current, then bacterium bag tightened；
S05: bacterium germination: being placed on temperature control for inoculated bacterium bag and cultivate in 28 DEG C of cleaning shop, and mycelia is in toothed oak wood wood
The covering of stake surface covers with and gos deep into wooden growth inside；
S06 fruit body development management: taking off bag for the good polypropylene 500*500 bacterium bag for installing timber of bacterium germination, sending out bacterium good
Toothed oak wood timber is placed in humidity 90%, 25 DEG C of temperature of cleaning shop is cultivated, until Antrodia camphorata fructification is long is to 1.5~3cm
It can pick, illumination is not necessarily in incubation.
Embodiment 3
The present embodiment provides a kind of culture medium prescription of Antrodia camphorata and its cultural method, the scheme carries out in accordance with the following steps:
S01: strain is obtained: purchase Antrodia camphorata strain, and be stored on PDA slant medium；
S02: preparation liquid spawn: 150ml liquid seed culture medium is added into the triangular flask of 500ml, then access activation
Antrodia camphorata strain, rotary shaker, revolving speed 160r/min cultivates 12 days under 26 DEG C of temperature of dark condition, obtains liquid
Antrodia camphorata strain；
S03: it prepares culture medium for cultivating: diameter 20cm is made in toothed oak ebon, the timber of length 20cm is put and is totally submerged leaching in water
Lignin, glucose, corn flour and potassium dihydrogen phosphate is added in bubble 30 days in soaking process, clear up bark after the completion of immersion and do
Only it is fitted into polypropylene 500*500 bacterium bag, bacterium bag mouth is locked with the plastic tube of diameter 10cm, then with 7 layers of non-woven fabrics by plastics
Pipe nozzle is sealed after packing tightly and is tightened, and is placed in 105 DEG C of autoclave sterilization tank after carrying out 12h sterilization in cleaning shop
Interior cooling；
S04: inoculation: on the superclean bench of cleaning shop, the liquid spawn fermented is shaken up, the bacterium bag cooled down is beaten
It opens, the Antrodia camphorata liquid spawn of 200ml or so is accessed with liquid inoculation rifle, make Antrodia camphorata liquid spawn can be from each of toothed oak wood timber
Under a section of surface current, then bacterium bag tightened；
S05: bacterium germination: being placed on temperature control for inoculated bacterium bag and cultivate in 24 DEG C of cleaning shop, and mycelia is in toothed oak wood wood
The covering of stake surface covers with and gos deep into wooden growth inside；
S06: fruit body development management: taking off bag for the good polypropylene 500*500 bacterium bag for installing timber of bacterium germination, sending out bacterium good
Toothed oak wood timber is placed in humidity 80%, 20 DEG C of temperature of cleaning shop is cultivated to Antrodia camphorata fructification length to 1.5~3cm i.e.
It can pick, illumination is not necessarily in incubation.
Embodiment 4
The present embodiment provides a kind of culture medium prescription of Antrodia camphorata and its cultural method, the scheme carries out in accordance with the following steps:
S01: strain is obtained: purchase Antrodia camphorata strain, and be stored on PDA slant medium；
S02: preparation liquid spawn: 150ml liquid seed culture medium is added into the triangular flask of 500ml, then access activation
Antrodia camphorata strain, rotary shaker, revolving speed 160r/min cultivates 13 days under 26 DEG C of temperature of dark condition, obtains liquid
Antrodia camphorata strain；
S03: it prepares culture medium for cultivating: diameter 20cm is made in toothed oak ebon, the timber of length 20cm is put and is totally submerged leaching in water
Lignin, glucose, corn flour and potassium dihydrogen phosphate is added in bubble 30 days in soaking process, clear up bark after the completion of immersion and do
Only it is fitted into polypropylene 500*500 bacterium bag, bacterium bag mouth is locked with the plastic tube of diameter 10cm, then with 7 layers of non-woven fabrics by plastics
Pipe nozzle is sealed after packing tightly and is tightened, and is placed in 105 DEG C of autoclave sterilization tank after carrying out 12h sterilization in cleaning shop
Interior cooling；
S04: inoculation: on the superclean bench of cleaning shop, the liquid spawn fermented is shaken up, the bacterium bag cooled down is beaten
It opens, the Antrodia camphorata liquid spawn of 200ml or so is accessed with liquid inoculation rifle, make Antrodia camphorata liquid spawn can be from each of toothed oak wood timber
Under a section of surface current, then bacterium bag tightened；
S05: bacterium germination: being placed on temperature control for inoculated bacterium bag and cultivate in 25 DEG C of cleaning shop, and mycelia is in toothed oak wood wood
The covering of stake surface covers with and gos deep into wooden growth inside；
S06: fruit body development management: taking off bag for the good polypropylene 500*500 bacterium bag for installing timber of bacterium germination, sending out bacterium good
Toothed oak wood timber is placed in humidity 75%, 22 DEG C of temperature of cleaning shop is cultivated to Antrodia camphorata fructification length to 1.5~3cm i.e.
It can pick, illumination is not necessarily in incubation.
The Antrodia camphorata that will be grown in the Antrodia camphorata fructification cultivated in above-described embodiment and the wild Cinnamomum kanahirai hay wood in Taiwan
It compares, mode of appearance is essentially identical, and triterpenes content and growth period comparison are as shown in table 1 below:
Table 1: the Antrodia camphorata fructification comparison of each embodiment growth
In above-mentioned table 1, comparative example 1 refers to the Antrodia camphorata grown on the wild Cinnamomum kanahirai hay wood in Taiwan, and as shown in Table 1, the present invention is implemented
The Antrodia camphorata triterpenes content that example 1 to embodiment 4 is prepared is between 15~40%, the Antrodia camphorata phase wild compared to Taiwan
Difference is very few, from original seed to the growth period for the Antrodia camphorata fructification for growing up to 1.5~3cm 1.0~1.5 years, compared to Taiwan
Wild Antrodia camphorata growth period shortens about half.
Certainly, above description is also not limited to the example above, technical characteristic of the present invention without description can by or
It is realized using the prior art, details are not described herein；It is not to this that the above examples are only used to illustrate the technical scheme of the present invention
The limitation of invention, describes the invention in detail referring to preferred embodiment, and those skilled in the art should
Understand, the variations, modifications, additions or substitutions that those skilled in the art are made within the essential scope of the present invention
Without departure from spirit of the invention, it also should belong to claims of the invention.
Claims (4)
1. the culture medium prescription and its cultural method of a kind of Antrodia camphorata, which is characterized in that described method includes following steps:
S01: strain is obtained: purchase Antrodia camphorata strain, and be stored on PDA slant medium；
S02: preparation liquid spawn: 150ml liquid seed culture medium is added into the triangular flask of 500ml, then described in access
The strain obtained in step S01, rotary shaker, revolving speed 160r/min, under 22~28 DEG C of temperature of dark condition cultivate 8~
16 days, obtain liquid Antrodia camphorata strain；
S02: it prepares culture medium for cultivating: diameter 20cm is made in toothed oak ebon, the timber of long 20cm is put and is totally submerged immersion in water
28 days, lignin 3%, glucose 2%, corn flour 1%, potassium dihydrogen phosphate 1.5% are added in soaking process；By bark after the completion of immersion
It cleans out and is fitted into polypropylene 500*500 bacterium bag, bacterium bag mouth is locked with the plastic tube of diameter 10cm, then with several layers of non-woven fabrics
Sealing tightens after plastic tube nozzle is packed tightly, and progress sterilization 12h under high-temperature and high-pressure conditions is placed in, then in cleaning shop
Interior cooling；
S03: inoculation: on the superclean bench of cleaning shop, the liquid spawn fermented is shaken up, the bacterium bag cooled down is beaten
It opens, accesses 120~200ml liquid Antrodia camphorata strain with liquid inoculation rifle, make Antrodia camphorata liquid spawn can be from each of toothed oak wood timber
Under a section of surface current, then bacterium bag tightened；
S04: bacterium germination: inoculated bacterium bag is placed on temperature control and is cultivated in 18~30 DEG C of cleaning shop, mycelia is in toothed oak
The covering of timber surface covers with and gos deep into wooden growth inside；
S05: fruit body development management: taking off bag for the good polypropylene 500*500 bacterium bag for installing timber of bacterium germination, sending out bacterium good
Toothed oak wood timber is placed in humidity 75~95%, 16~28 DEG C of temperature of cleaning shop is cultivated, and illumination is not necessarily in incubation.
2. the method for cultivation Antrodia camphorata fructification according to claim 1, which is characterized in that in the step S03, fermentation
Good liquid spawn is the liquid Antrodia camphorata strain obtained in the step S02 to be accessed sterilizing and fermentor after cooling is primary
On fermentation medium, 22~28 DEG C of constant temperature are kept in fermentor, are passed through filtrated air culture 6~12 days and are obtained.
3. the method for cultivation Antrodia camphorata fructification according to claim 2, which is characterized in that the fermentor one time fermentation
Culture medium includes the component of following mass fraction: wheat bran: 5%, corn flour: 3%. wheat powder: 8%. glucose: 1.5%, KH2P04:
0.1%, MgS04: 0.15%, peptone: 0.2%, surplus is water.
4. the method for cultivation Antrodia camphorata fructification according to claim 3, which is characterized in that described in the step S02
Liquid seed culture medium includes: carrot 200g, glucose 20g, water 1000ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710544301.4A CN109418003A (en) | 2017-07-06 | 2017-07-06 | The culture medium prescription and its cultural method of Antrodia camphorata |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710544301.4A CN109418003A (en) | 2017-07-06 | 2017-07-06 | The culture medium prescription and its cultural method of Antrodia camphorata |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109418003A true CN109418003A (en) | 2019-03-05 |
Family
ID=65498678
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710544301.4A Pending CN109418003A (en) | 2017-07-06 | 2017-07-06 | The culture medium prescription and its cultural method of Antrodia camphorata |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109418003A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109757300A (en) * | 2019-03-14 | 2019-05-17 | 安佑生物科技集团股份有限公司 | A kind of breeding method of Antrodia camphorata and its application in animal feed |
-
2017
- 2017-07-06 CN CN201710544301.4A patent/CN109418003A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109757300A (en) * | 2019-03-14 | 2019-05-17 | 安佑生物科技集团股份有限公司 | A kind of breeding method of Antrodia camphorata and its application in animal feed |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102187787B (en) | Method for culturing rare edible fungi including tricholoma lobayense heim and clitocybe maxima by using vine shoot dust | |
| CN102037849B (en) | Tremella fuciformis strain culture method | |
| CN106613355A (en) | Ecological planting method of oyster mushrooms | |
| CN105210671A (en) | A kind of log glossy ganoderma breeding method | |
| CN105906415A (en) | Method for cultivating antrodia camphorata fruiting bodies by use of applewood and culture medium | |
| CN104969773B (en) | The method for obtaining Cordyceps militaris fermentate or fructification using sweet potato dregs fermentation | |
| CN105875197A (en) | Agaricus bisporus cultivation method | |
| TWI393530B (en) | The method of inoculation and cultivation of | |
| CN102523917A (en) | Method for cultivating straw mushroom | |
| KR101174245B1 (en) | Lentinus edode cultivated by using fermentation medium and cultvation method thereof | |
| CN102612994B (en) | Method for cultivating oyster mushroom cultivation by directly mixing liquid spawns with raw materials | |
| KR100823541B1 (en) | Mushroom cultivation method | |
| KR101056952B1 (en) | Mass production method of black scale mushroom using lumber cultivation | |
| JP2007053928A (en) | New fungal strain and method for culturing new fruit body | |
| CN104126414A (en) | Black fungus artificial cultivation method | |
| CN106856984A (en) | A kind of Hydnum tree and its cultural method | |
| CN102286385B (en) | Method for carrying out solid culturing on beauveria bassiana by stages | |
| CN107253872A (en) | A kind of culture medium and its cultural method for being used to cultivate Antrodia camphorata fructification | |
| CN109418003A (en) | The culture medium prescription and its cultural method of Antrodia camphorata | |
| CN103070015A (en) | Method for cultivating cut flower glossy ganoderma | |
| CN103858659A (en) | Bag cultivation method of black fungi | |
| CN110229757A (en) | One plant effectively facilitates the tangerine green trichoderma JS84 of plant growth and its biological organic fertilizer of development | |
| CN109247190A (en) | A kind of white ginseng bacterial strain and its white ginseng short cycle industrial planting method | |
| CN110122182A (en) | A kind of oil tea mushroom chaff prepares Grifola frondosa culture material and preparation method thereof | |
| CN111758484B (en) | Large-scale high-efficiency high-yield silkworm chrysalis cordyceps production and cultivation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20190305 |