CN109757300A - A kind of breeding method of Antrodia camphorata and its application in animal feed - Google Patents
A kind of breeding method of Antrodia camphorata and its application in animal feed Download PDFInfo
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Abstract
Application the invention discloses a kind of breeding method of Antrodia camphorata and its in animal feed, breeding method includes the following steps: 1) to make Antrodia camphorata culture medium, wherein the Antrodia camphorata culture medium includes butt and auxiliary material, the butt includes corn and rice, the auxiliary material includes at least tryptone, yeast extract, leaf of Cortex cinnamomi camphorae powder and water, and the Antrodia camphorata culture medium is obtained by mixing by the butt and the auxiliary material；2) the Antrodia camphorata culture medium of preparation is subjected to sterilization treatment, be depressured, it is cooling；3) Antrodia camphorata culture medium after cooling is placed in clean work station, Antrodia camphorata strain is accessed after ultraviolet light, mixture is obtained after mixing, the mixture is placed in culture apparatus and is cultivated, after 25-50 days, that is, obtain tunning Antrodia camphorata；And above-mentioned breeding method cultivates application of the Antrodia camphorata obtained in animal feed；Method of the invention can shorten cultivation period, reduce in cultivating process by the risk of other living contaminants.
Description
Technical field
The invention belongs to animal feed additive field more particularly to a kind of Antrodia camphoratas, and in particular to a kind of Antrodia camphorata
Breeding method and its application in animal feed.
Background technique
Antrodia camphorata also known as cinnamomum kanahirai hay mushroom, belong to Aphyllophorales, Polyporaceae, antrodia karst, perennial gill fungus mushroom, and Latin is entitled
Antrodia camphorata, is the novel species for just finding name nineteen ninety, and growth district is Taiwan mountain area height above sea level 450-2000
Between meter Shan Lin, and it is grown in the rotten heartwood inner wall of cinnamomum kanehirai trunk of distinctive a century in Taiwan or more.Wild Antrodia camphorata is long
In aged cinnamomum kanehirai, sporophore shape changeableization has plate, clock shape, horse-hof shape or tower-like；Nascent cerise is gradually long to become
For white, reddish tan, filbert or khaki.Its gas fragrance, acrid flavour are bitter/flat, have wind-dispelling promoting the circulation of qi, change become silted up promoting blood circulation, middle benefit gas
The effect of knot, removing toxicity for detumescence, sedation-analgesia, antibacterial, antiviral and antitumor of disappearing；For treating gastrointestinal distress, diarrhea and vomiting, food
Object poisoning, mushroom poisoning, diabetes, alcoholic liver, fatty liver, cirrhosis, liver cancer etc. are even more to have unique function.Antrodia camphorata, which has, to be permitted
More physiologically active ingredients, as polysaccharide body, triterpene compound, Sudismase, adenosine, vitamin, microelement, nucleic acid,
Agglutinin, amino acid, solid liquor-saturated tired, cellulose, blood pressure stabilization substance etc..
Antrodia camphorata is considered as unique and precious medicinal fungi in Taiwan, therefore has high research and commercial value,
It is also the wild fungi of current Taiwan most expensive, is referred to as " refreshing sesame " in Hongkong and Macro, Taiwan is civil is referred to as " the red treasured in forest
Stone ".Simultaneously because wild Antrodia camphorata generated surprising curative effect and wild raw material resources in Popular Utilization are exhausted, not only make
Its price is surging, and excites the strong interest of government and major research institution, Sheng Ji company！Between a period of time, various utilizations
Biochemical technology cultivates the research of antrodia and makes great efforts to become biochemical technology project the most flourishing！
Currently, can substantially be divided into cultivation basswood method, plate method, solution fermentation three for Antrodia camphorata artificial culture
Kind, at the same the Antrodia camphorata active constituent cultivated of different breeding method can there is certain difference, application field there is also
Difference.However above-mentioned three kinds of methods the problem of although can solve Antrodia camphorata shortage of resources, but this three kinds of breeding methods are still also
There is the following problems, such as that there is cultivating processes is more slow, and growth cycle is long, higher cost, there is also
Pollution in incubation vulnerable to other miscellaneous bacterias causes effective component to be greatly diminished the problem of even fermentation fails, because
This, those skilled in the art urgently seek it is a kind of can be improved fermenting speed and have both can be avoided by other living contaminants
Breeding method.
Summary of the invention
The technical problem to be solved by the present invention is to overcome the deficiencies of the prior art and provide a kind of new side for cultivating Antrodia camphorata
Method enables to Antrodia camphorata Rapid Fermentation to sprout, and shortens fermentation period, and can reduce ox early period existing in the prior art
Antrodia slow growth and the problem of lead to other living contaminants, improve the content of effective component in Antrodia camphorata.
The present invention also provides a kind of application of the Antrodia camphorata of said new method cultivation in animal feed, can be improved
The immunity of animal.
In order to solve the above technical problems, a kind of technical solution that the present invention takes is as follows:
A kind of breeding method of Antrodia camphorata, the breeding method include the following steps:
(1) Antrodia camphorata culture medium is made, wherein the Antrodia camphorata culture medium includes butt and auxiliary material, the butt and described
The mass ratio that feeds intake of auxiliary material is 1 ︰ 0.4-0.9, and the butt includes corn and rice, the auxiliary material include at least tryptone,
Yeast extract, leaf of Cortex cinnamomi camphorae powder and water, the Antrodia camphorata culture medium are obtained by mixing by the butt and the auxiliary material；
(2) Antrodia camphorata culture medium prepared by step (1) is gone out at 100-150 DEG C of temperature, pressure 0.01-0.3Mpa
Bacterium processing, is depressured, cooling；
(3) step (2) Antrodia camphorata culture medium after cooling is placed in clean work station, Cinnamomum kanahirai hay is accessed after ultraviolet light
The quality of sesame strain, the Antrodia camphorata strain accounts for the 5-20% of the butt quality, mixture is obtained after mixing, by the mixture
It is placed in culture apparatus and cultivates, 25-50 day after tomorrow, i.e. acquisition tunning Antrodia camphorata.
Some preferred aspects according to the present invention, in step (1), the mass ratio that feeds intake of the butt and the auxiliary material is 1 ︰
0.6-0.7。
Some preferred aspects according to the present invention, in step (1), in terms of mass percentage, in the butt, corn is accounted for
50-70%, rice account for 30-50%.
Some specific aspects according to the present invention, in step (1), the corn can select hominy grits, and the rice can
It is cracked rice with selecting.
Some preferred aspects according to the present invention, in step (1), the auxiliary material further includes glucose, magnesium sulfate and phosphoric acid
Potassium dihydrogen.
Further, in step (1), in terms of mass percentage, in the auxiliary material, tryptone accounts for 0.3-0.8%, ferment
Female extract accounts for 0.3-0.8%, leaf of Cortex cinnamomi camphorae powder accounts for 1-5%, glucose accounts for 1-4%, magnesium sulfate accounts for 0.05-0.20%, phosphoric acid
Potassium dihydrogen accounts for 0.05-0.30%, and surplus is water.
A specific aspect according to the present invention, in step (1), the yeast extract can be yeast extract.
A specific aspect according to the present invention, in step (1), the water can be distilled water.
Some preferred aspects according to the present invention in step (2), control the sterilization treatment in 115-125 DEG C of temperature, pressure
It is carried out under power 0.1-0.2Mpa.
Some specific aspects according to the present invention, in step (2), the time for controlling the sterilization treatment is 10-30min.
In terms of more according to the present invention specific and preferred, in step (3), the temperature for controlling culture is 22-30 DEG C.
Some preferred aspects according to the present invention, in step (3), the culture apparatus includes for loading the mixture
Container, sealing cloth for opening and closing the container, and the opening for the sealing cloth to be fixed on to the container
Locking mechanism, the sealing cloth are formed by stacking by multilayer gauze.
It is further preferred that the container is the bottle of PP polybag or PP material, autoclave sterilization can be suitble to,
And can reuse, cost is also cheaper.
It is further preferred that the locking mechanism can be fixed rope, the fixed rope can be cotton cord, rubber band.
It is further preferred that the sealing cloth is formed by stacking by 16-20 layers of gauze, one side can guarantee effectively to filter
On the other hand miscellaneous bacteria in air can also guarantee that air permeability is good, meet amount of oxygen needed for Antrodia camphorata growth is fermented.
Culture apparatus of the invention, on the one hand, it is not necessary that culture apparatus is placed in gnotobasis (higher cost), another party
Face, compared with the existing technology in culture device may be implemented preferably to completely cut off miscellaneous bacteria and be passed through the purpose of sufficient amount of oxygen.
Another technical solution provided by the invention: a kind of breeding method of Antrodia camphorata described above cultivates the Cinnamomum kanahirai hay of acquisition
Application of the sesame in animal feed.
Animal feed of the invention can be used for pig feeding, chicken, duck, fish, goose, pigeon, ox, sheep, horse, dog, cat, deer and rabbit etc.
Deng.
Due to the use of above technical scheme, the invention has the following advantages over the prior art:
The new method of cultivation Antrodia camphorata of the invention, enables to Antrodia camphorata Rapid Fermentation to sprout, shortens fermentation period,
And the problem of can reduce Antrodia camphorata early period slow growth existing in the prior art and leading to other living contaminants, it has both simultaneously
The content that effective component in Antrodia camphorata can be improved can be applied in animal feed and significantly improve the immunity of animal；Together
When the present invention fermented using the cereal materials cooperation auxiliary material such as corn, rice, be not necessarily to camphor tree linden material, be conducive to expand life
It produces, avoids the drawbacks of destroying forest of falling trees, and cost is relatively low, easy to operate, therefore, the present invention overcomes in the prior art
Existing growth cycle length, susceptible, problem at high cost, effective content is low.
Specific embodiment
Below with reference to specifically preferred embodiment mode, the present invention is further explained.It should be understood that these embodiments are only used for
The range illustrated the present invention and not to limit the present invention.In addition, after reading the contents of the present invention, those skilled in the art
The present invention can be made various changes or modifications, such equivalent forms equally fall within limited range of the present invention.
In the following embodiments, unless otherwise specified, all raw materials can be commercially available.Antrodia camphorata strain
(Antrodia cinnamomea KGbio AC003) is purchased from Taiwan Jing Guan Biotechnology Co., Ltd.
Embodiment 1
The present embodiment provides a kind of breeding method of Antrodia camphorata, the breeding method includes the following steps:
(1) Antrodia camphorata culture medium is made, wherein the Antrodia camphorata culture medium includes butt and auxiliary material, the butt and described
The mass ratio that feeds intake of auxiliary material is 1 ︰ 0.65, and the butt includes hominy grits 965g and the 645g that cracks rice, and the auxiliary material includes tryptose
Peptone 5g, yeast extract 5g, leaf of Cortex cinnamomi camphorae powder 10g, glucose 25g, magnesium sulfate 1g, potassium dihydrogen phosphate 1.5g and distilled water 1L, institute
Antrodia camphorata culture medium is stated to be obtained by mixing by the butt and the auxiliary material；
(2) Antrodia camphorata culture medium prepared by step (1) is subjected to sterilization treatment at 121 DEG C of temperature, pressure 0.15Mpa
20min is depressured naturally, cooling；
(3) step (2) Antrodia camphorata culture medium after cooling is placed in clean work station, ultraviolet light 30min is followed by
Enter Antrodia camphorata liquid spawn, the quality of the Antrodia camphorata strain accounts for the 10% of the butt quality, mixture is obtained after mixing, by institute
It states mixture and is placed in culture apparatus at 26 ± 2 DEG C and cultivate, obtain tunning Antrodia camphorata within 30 days；
Wherein, the culture apparatus include for loading the container of the mixture, the sealing for opening and closing the container
Cloth, and the locking mechanism of the opening for the sealing cloth to be fixed on to the container, the sealing cloth is by multilayer gauze
It is formed by stacking.Specifically, in this example, the container is PP material polybag, can be suitble to autoclave sterilization, and can repeat to make
With, cost is also cheaper, the locking mechanism can be fixed rope, the fixed rope can be rubber band, the sealing cloth by
18 layers of gauze are formed by stacking.
In this example, during cultivating Antrodia camphorata, the cultivation fermentation process of fast speed is realized, can be obtained within 30 days
Tunning Antrodia camphorata, and the problem of there is no by other living contaminants, while also without being cultivated using sterile board, greatly
Ground reduces costs.
Embodiment 2
The present embodiment provides a kind of breeding method of Antrodia camphorata, the breeding method includes the following steps:
(1) Antrodia camphorata culture medium is made, wherein the Antrodia camphorata culture medium includes butt and auxiliary material, the butt and described
The mass ratio that feeds intake of auxiliary material is 1 ︰ 0.65, and the butt includes hominy grits 990g and the 660g that cracks rice, and the auxiliary material includes tryptose
Peptone 8g, yeast extract 8g, leaf of Cortex cinnamomi camphorae powder 20g, glucose 35g, magnesium sulfate 1.5g, potassium dihydrogen phosphate 2g and distilled water 1L, institute
Antrodia camphorata culture medium is stated to be obtained by mixing by the butt and the auxiliary material；
(2) Antrodia camphorata culture medium prepared by step (1) is subjected to sterilization treatment at 121 DEG C of temperature, pressure 0.15Mpa
20min is depressured naturally, cooling；
(3) step (2) Antrodia camphorata culture medium after cooling is placed in clean work station, ultraviolet light 30min is followed by
Enter Antrodia camphorata liquid spawn, the quality of the Antrodia camphorata strain accounts for the 15% of the butt quality, mixture is obtained after mixing, by institute
It states mixture and is placed in culture apparatus at 26 ± 2 DEG C and cultivate, obtain tunning Antrodia camphorata within 25 days；
Wherein, the culture apparatus include for loading the container of the mixture, the sealing for opening and closing the container
Cloth, and the locking mechanism of the opening for the sealing cloth to be fixed on to the container, the sealing cloth is by multilayer gauze
It is formed by stacking.Specifically, in this example, the container is PP material polybag, can be suitble to autoclave sterilization, and can repeat to make
With, cost is also cheaper, the locking mechanism can be fixed rope, the fixed rope can be rubber band, the sealing cloth by
18 layers of gauze are formed by stacking.
In this example, during cultivating Antrodia camphorata, the cultivation fermentation process of fast speed is realized, can be obtained within 25 days
Tunning Antrodia camphorata, and the problem of there is no by other living contaminants, while also without being cultivated using sterile board, greatly
Ground reduces costs.
Embodiment 3
The present embodiment provides a kind of breeding method of Antrodia camphorata, the breeding method includes the following steps:
(1) Antrodia camphorata culture medium is made, wherein the Antrodia camphorata culture medium includes butt and auxiliary material, the butt and described
The mass ratio that feeds intake of auxiliary material is 1 ︰ 0.6, and the butt includes hominy grits 890g and the 890g that cracks rice, and the auxiliary material includes tryptone
5g, yeast extract 5g, leaf of Cortex cinnamomi camphorae powder 30g, glucose 25g, magnesium sulfate 1g, potassium dihydrogen phosphate 1.5g and distilled water 1L, it is described
Antrodia camphorata culture medium is obtained by mixing by the butt and the auxiliary material；
(2) Antrodia camphorata culture medium prepared by step (1) is subjected to sterilization treatment at 121 DEG C of temperature, pressure 0.15Mpa
30min is depressured naturally, cooling；
(3) step (2) Antrodia camphorata culture medium after cooling is placed in clean work station, ultraviolet light 30min is followed by
Enter Antrodia camphorata liquid spawn, the quality of the Antrodia camphorata strain accounts for the 20% of the butt quality, mixture is obtained after mixing, by institute
It states mixture and is placed in culture apparatus at 26 ± 2 DEG C and cultivate, obtain tunning Antrodia camphorata within 50 days；
Wherein, the culture apparatus include for loading the container of the mixture, the sealing for opening and closing the container
Cloth, and the locking mechanism of the opening for the sealing cloth to be fixed on to the container, the sealing cloth is by multilayer gauze
It is formed by stacking.Specifically, in this example, the container is the bottle of PP material, can be suitble to autoclave sterilization, and can repeat to make
With, cost is also cheaper, the locking mechanism can be fixed rope, the fixed rope can be rubber band, the sealing cloth by
20 layers of gauze are formed by stacking.
In this example, during cultivating Antrodia camphorata, the cultivation fermentation process of fast speed is realized, can be obtained within 50 days
Tunning Antrodia camphorata, and the problem of there is no by other living contaminants, while also without being cultivated using sterile board, greatly
Ground reduces costs.
Comparative example 1
Linden fermentation: be using cinnamomum kanehirai linden or other camphor tree lindens as raw material, by cutting, polishing, it is soaked deoil, it is high
The links such as temperature sterilizing, inoculation, culture are prepared.But government increases the protection to cinnamomum kanehirai in recent years, forbids illegally buying
Cinnamomum kanehirai linden is sold, raw material resources are rare to cause cost to increase considerably, and linden fermentation period is longer, generally needs 1-3.Though
Right linden fermentation is relatively similar with wild Antrodia camphorata ingredient, but high cost limits the application of Antrodia camphorata, can only be applied to people on a small quantity
Class health products trade.
Comparative example 2
Liquid fermentation: being to carry out high-volume life by industrial fermentation tank with raw materials such as peptone, yeast extract, fructose
It produces, general culture 14-30 days.Liquid fermentation has and can amplify production and advantage with short production cycle, but by condition of culture
Limitation, liquid fermentation metabolite type is relatively single, generates minimal amount of or does not generate triterpene compound, does not generate MBDD
Ingredient.
Application example 1
Fermentation material Antrodia camphorata prepared by embodiment 1 is with effective component MBDD (dioxane between 5- methyl-benzo [1,3]
Amylene -4,7- glycol) meter, be added in animal feed, make in feed Antrodia camphorata effective component MBDD content reach 50~
900mg/T。
Application example 2
26 age in days weanling pigs similar in 48 weight are selected, 2 groups, every group of 3 repetitions, each repetition 8 are randomly divided into
Head pig；Control group fed conventional feed, test group add the Antrodia camphorata fermentation of the preparation of the embodiment of the present invention 1 in conventional feed
Object counts two groups of feed feed intakes, piglets so that Antrodia camphorata effective component MBDD content 100mg/T in testing material, feeds 28 days
Average daily gain, feed-weight ratio, diarrhea rate, the results are shown in Table 1.
Table 1 adds influence of the Antrodia camphorata to child care phase piglet growth performance in child care material
Data shoulder mark go together without letter or not significant (P > 0.05), the different small letters containing identical lowercase letter indication difference
Letter indicates significant difference (P < 0.05).
Application example 3
26 age in days weanling pigs similar in 48 weight are selected, 2 groups, every group of 3 repetitions, each repetition 8 are randomly divided into
Head pig；Control group fed conventional feed, test group add the Antrodia camphorata fermentation of the preparation of the embodiment of the present invention 1 in conventional feed
Object counts two groups of feed feed intakes, piglets so that Antrodia camphorata effective component MBDD content 200mg/T in testing material, feeds 28 days
Average daily gain, feed-weight ratio, diarrhea rate, the results are shown in Table 2.
Table 2 adds influence of the Antrodia camphorata to child care phase piglet growth performance in child care material
Application example 4
26 age in days weanling pigs similar in 48 weight are selected, 2 groups, every group of 3 repetitions, each repetition 8 are randomly divided into
Head pig；Control group fed conventional feed, test group add the Antrodia camphorata fermentation of the preparation of the embodiment of the present invention 1 in conventional feed
Object counts two groups of feed feed intakes, piglets so that Antrodia camphorata effective component MBDD content 300mg/T in testing material, feeds 28 days
Average daily gain, feed-weight ratio, diarrhea rate, the results are shown in Table 3.
Table 3 adds influence of the Antrodia camphorata to child care phase piglet growth performance in child care material
Application example 5
26 age in days weanling pigs similar in 96 weight are selected, 4 groups, every group of 3 repetitions, each repetition 8 are randomly divided into
Head pig；Control group (A group) feeds conventional feed, and test group (B-D group) adds Antrodia camphorata prepared by the present invention in conventional feed
Fermentation material, Antrodia camphorata content (in terms of MBDD) is respectively 0,100,200,300ppb in each group piglet diet.Respectively in experiment opening
Begin, on the day of off-test, each repeating groups randomly select test piglet 2, and vena cava anterior acquires 5mL blood sample, is stored at room temperature analysis
3000rPm/min centrifugation 15min prepares serum at 4 DEG C after serum out, after taking a blood sample for the first time, marks to blood sampling pig, after guarantee
It is same head pig that face, which was taken a blood sample twice with first time, measurement interleukin 8 (IL-8), interleukin 12 (IL-12), interference
Plain γ (IFN-γ).
Table 4 adds influence of the Antrodia camphorata to piglet serum cytokines content
Data shoulder mark go together without letter or not significant (P > 0.05), the different small letters containing identical lowercase letter indication difference
Letter indicates significant difference (P < 0.05), and following table is same.
Interleukin 8 (IL-8) is the chemotactic factor (CF) of a kind of neutrophil leucocyte, T cell and basophilic granulocyte, is
The important medium of diseases associated with inflammation adjusts in anti-infective, immune response and anti-tumor aspect plays an important role.As a kind of master
The inflammatory factor wanted, IL-8 level is in the case where infection and certain autoimmune diseases in inflammation part, serum and body fluid
In dramatically increase.IL-8 concentration shows that IL-8 may be straight with the size of tumour and the significant correlation of tumor stage in serum
Connect or take part in indirectly the development (Y.Ren et al.2013) of liver cancer.Correlative study is shown in Antrodia camphorata in vitro test and extracts
Taking object is the concentration that can reduce IL-8, and in this test, it is low to add IL-8 level in each test group piglet 14d blood of Antrodia camphorata
In control group.IL-8 can stimulate the chemotactic of neutrophil leucocyte, T lymphocyte and eosinophil, promote neutrophil leucocyte de-
Particle discharges elastoser, and endothelial cell injury makes microcirculation blood flow be retarded by silt, and tissue necrosis causes Multi-organ dysfunction.
Interleukin 12 (IL-12) is mainly derived from Dendritic Cells, macrophage and bone-marrow-derived lymphocyte, is a kind of energy
The inflammatory factor that immune response is enough generated for pathogenic microorganism, participate in organism infection, tumour, autoimmune disease it is immune
It adjusts.IL-12 adjust helper lymphocyte T Th1 development, proliferation and Th1/Th2 cell balance, inducer T lymphocyte and
Natural killer cells generates interferon (IFN-γ), and IFN-γ generates carbon monoxide and enzyme, discharges active nitrogen, causes to swallow function
It can be with local inflammatory response enhancing.The display of this test result, Antrodia camphorata can reduce the amount of inflammatory factor IL-12 and IFN-γ, rise
To adjusting immunization.
The above are the descriptions to the embodiment of the present invention to keep this field special by the foregoing description of the disclosed embodiments
Industry technical staff can be realized or using the present invention.Various modifications to these embodiments carry out those skilled in the art
Saying will be apparent, and the general principles defined herein can be the case where not departing from the spirit or scope of the present invention
Under, it realizes in other embodiments.Therefore, the present invention will not be limited to the embodiments shown herein, but to accord with
Close the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. a kind of breeding method of Antrodia camphorata, which is characterized in that the breeding method includes the following steps:
(1) Antrodia camphorata culture medium is made, wherein the Antrodia camphorata culture medium includes butt and auxiliary material, the butt and the auxiliary material
The mass ratio that feeds intake be 1 ︰ 0.4-0.9, the butt includes corn and rice, and the auxiliary material includes at least tryptone, yeast
Extract, leaf of Cortex cinnamomi camphorae powder and water, the Antrodia camphorata culture medium are obtained by mixing by the butt and the auxiliary material；
(2) Antrodia camphorata culture medium prepared by step (1) is carried out at sterilizing at 100-150 DEG C of temperature, pressure 0.01-0.3Mpa
Reason is depressured, cooling；
(3) step (2) Antrodia camphorata culture medium after cooling is placed in clean work station, Antrodia camphorata bacterium is accessed after ultraviolet light
Kind, the quality of the Antrodia camphorata strain accounts for the 5-20% of the butt quality, and mixture is obtained after mixing, the mixture is placed in
It is cultivated in culture apparatus, after 25-50 days, that is, obtains tunning Antrodia camphorata.
2. the breeding method of Antrodia camphorata according to claim 1, which is characterized in that in step (1), the butt and described
The mass ratio that feeds intake of auxiliary material is 1 ︰ 0.6-0.7.
3. the breeding method of Antrodia camphorata according to claim 1, which is characterized in that in step (1), with mass percentage
It counts, in the butt, corn accounts for 50-70%, and rice accounts for 30-50%.
4. the breeding method of Antrodia camphorata according to claim 1, which is characterized in that in step (1), the auxiliary material further includes
Glucose, magnesium sulfate and potassium dihydrogen phosphate.
5. the breeding method of Antrodia camphorata according to claim 4, which is characterized in that in step (1), with mass percentage
It counts, in the auxiliary material, tryptone accounts for 0.3-0.8%, yeast extract accounts for 0.3-0.8%, leaf of Cortex cinnamomi camphorae powder accounts for 1-5%, glucose
Account for 1-4%, magnesium sulfate accounts for 0.05-0.20%, potassium dihydrogen phosphate accounts for 0.05-0.30%, surplus is water.
6. the breeding method of Antrodia camphorata according to claim 1, which is characterized in that in step (2), control at the sterilizing
Reason is carried out at 115-125 DEG C of temperature, pressure 0.1-0.2Mpa.
7. the breeding method of Antrodia camphorata according to claim 1, which is characterized in that in step (2), control at the sterilizing
The time of reason is 10-30min.
8. the breeding method of Antrodia camphorata according to claim 1, which is characterized in that in step (3), control the temperature of culture
It is 22-30 DEG C.
9. the breeding method of Antrodia camphorata according to claim 1, which is characterized in that in step (3), the culture apparatus packet
The container for loading the mixture, the sealing cloth for opening and closing the container are included, and for fixing the sealing cloth
In the locking mechanism of the opening of the container, the sealing cloth is formed by stacking by multilayer gauze.
10. the breeding method of Antrodia camphorata described in a kind of any one of claim 1-9 claim cultivates the Antrodia camphorata obtained
Application in animal feed.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113367252A (en) * | 2020-03-09 | 2021-09-10 | 张腾龙 | Use of nutritional composition |
| CN115152529A (en) * | 2022-08-18 | 2022-10-11 | 夏兴林 | Planting and cultivating method of ganoderma lucidum |
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