CN109415765A - 用纳米孔的聚合物分析中的混合光学信号 - Google Patents

用纳米孔的聚合物分析中的混合光学信号 Download PDF

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Publication number
CN109415765A
CN109415765A CN201780023542.2A CN201780023542A CN109415765A CN 109415765 A CN109415765 A CN 109415765A CN 201780023542 A CN201780023542 A CN 201780023542A CN 109415765 A CN109415765 A CN 109415765A
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CN
China
Prior art keywords
nano
pore
fluorescent marker
monomer
polynucleotides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201780023542.2A
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English (en)
Chinese (zh)
Inventor
布雷特·N·安德森
马丁·胡伯
斯蒂芬·C·梅斯维兹
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Quantapore Inc
Original Assignee
Quantapore Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Quantapore Inc filed Critical Quantapore Inc
Publication of CN109415765A publication Critical patent/CN109415765A/zh
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00—Nucleic acid detection characterised by assay method
    • C12Q2561/113—Real time assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6432—Quenching

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
CN201780023542.2A 2016-04-14 2017-03-27 用纳米孔的聚合物分析中的混合光学信号 Pending CN109415765A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662322343P 2016-04-14 2016-04-14
US62/322,343 2016-04-14
PCT/US2017/024314 WO2017180319A1 (fr) 2016-04-14 2017-03-27 Signaux optiques mixtes dans une analyse de polymère à l'aide de nanopores

Publications (1)

Publication Number Publication Date
CN109415765A true CN109415765A (zh) 2019-03-01

Family

ID=60042715

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201780023542.2A Pending CN109415765A (zh) 2016-04-14 2017-03-27 用纳米孔的聚合物分析中的混合光学信号

Country Status (5)

Country Link
US (2) US20190078158A1 (fr)
EP (1) EP3443122B1 (fr)
JP (1) JP2019513387A (fr)
CN (1) CN109415765A (fr)
WO (1) WO2017180319A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023029044A1 (fr) * 2021-09-06 2023-03-09 百图生科(北京)智能技术有限公司 Méthode et appareil de séquençage de cellule unique, dispositif, support et produit-programme

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019528065A (ja) * 2016-08-19 2019-10-10 クアンタポール， インコーポレイテッド 消光剤を使用する光学に基づくナノポア配列決定
GB201902630D0 (en) * 2019-02-27 2019-04-10 Imperial Innovations Ltd Analyte detection method

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130040827A1 (en) * 2011-08-14 2013-02-14 Stephen C. Macevicz Method and compositions for detecting and sequencing nucleic acids
US20140255935A1 (en) * 2009-09-30 2014-09-11 Quantapore, Inc. Ultrafast sequencing of biological polymers using a labeled nanopore
CN105283560A (zh) * 2013-05-24 2016-01-27 昆塔波尔公司 基于纳米孔的通过混合的fret检测的核酸分析

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7575865B2 (en) * 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
WO2012121756A1 (fr) * 2011-03-04 2012-09-13 Quantapore, Inc. Appareil et procédé pour effectuer la détection ou le séquençage optique de nanopores
WO2014066905A2 (fr) * 2012-10-28 2014-05-01 Quantapore, Inc. Réduction de fluorescence d'arrière-plan dans des matériaux mems par traitement par faisceau d'ions de faible énergie
CA2963604C (fr) * 2014-10-10 2023-02-14 Quantapore, Inc. Analyse de polymeres, a base de nanopore, a l'aide de marqueurs fluorescents a desactivation mutuelle
US20170132361A1 (en) * 2015-10-28 2017-05-11 Pacific Biosciences Of California, Inc. Sequence assembly method
JP6967006B2 (ja) * 2016-01-15 2021-11-17 クアンタポール， インコーポレイテッド 低減されたバックグラウンドの光学に基づくナノポア分析
EP3523455A4 (fr) * 2016-10-10 2020-06-17 Quantapore Inc. Contrôle d'une composition de signaux fluorescents dans un séquençage de nanopores à base optique

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140255935A1 (en) * 2009-09-30 2014-09-11 Quantapore, Inc. Ultrafast sequencing of biological polymers using a labeled nanopore
US20130040827A1 (en) * 2011-08-14 2013-02-14 Stephen C. Macevicz Method and compositions for detecting and sequencing nucleic acids
CN105283560A (zh) * 2013-05-24 2016-01-27 昆塔波尔公司 基于纳米孔的通过混合的fret检测的核酸分析

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ANDREW M SMITH等: "Highly-multiplexed barcode sequencing: an efficient method for parallel analysis of pooled samples", 《NUCLEIC ACIDS RESEARCH》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023029044A1 (fr) * 2021-09-06 2023-03-09 百图生科(北京)智能技术有限公司 Méthode et appareil de séquençage de cellule unique, dispositif, support et produit-programme

Also Published As

Publication number Publication date
EP3443122B1 (fr) 2021-07-07
US20190078158A1 (en) 2019-03-14
US20220170088A1 (en) 2022-06-02
JP2019513387A (ja) 2019-05-30
EP3443122A4 (fr) 2019-12-04
EP3443122A1 (fr) 2019-02-20
WO2017180319A1 (fr) 2017-10-19

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