CN109392895B - Rubber tree bud strip preservation method suitable for transportation - Google Patents
Rubber tree bud strip preservation method suitable for transportation Download PDFInfo
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- CN109392895B CN109392895B CN201811457779.4A CN201811457779A CN109392895B CN 109392895 B CN109392895 B CN 109392895B CN 201811457779 A CN201811457779 A CN 201811457779A CN 109392895 B CN109392895 B CN 109392895B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
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Abstract
The invention discloses a method for storing rubber tree buds suitable for transportation, which comprises the following steps: cutting off the rubber tree buds, cutting off tender tips at the top ends, and uniformly coating the latex seeped from the notches at the two ends; cutting off all leaves, and uniformly smearing the petiole cut with latex when each leaf is cut off; dipping latex of the rubber tree by using a cotton ball, uniformly coating the latex on the surface of the buds to seal all parts of the buds, and coating the latex after every few minutes to ensure that the thickness of the latex layer covered on the surface of the buds is more than 0.2 mm; after waiting for several minutes, winding and wrapping the buds by using a transparent adhesive tape when the latex on the surfaces of the buds is not sticky, and putting the buds into a plastic bottle which is not easy to deform; the plastic bottle can be stored and transported by being placed in a foam box filled with ice bags. The materials used in the invention are easy to obtain and the cost is lower; the buds are not easy to lose water or be extruded in the process of storage and transportation; the activity of the bud point is still more than 80% after the bud sticks are stored for 5-6 days, and the method is suitable for long-distance transportation.
Description
Technical Field
The invention belongs to the technical field of agricultural biology, and particularly relates to a preservation method of rubber tree buds suitable for transportation.
Background
Natural rubber is an important industrial raw material and strategic resource, and the rubber tree is the only natural rubber source plant which is commercially planted on a large scale at present. Although the rubber tree originates from the river basin of amazon south america, the main planting area of the rubber tree currently covers a plurality of countries and regions such as thailand, indonesia, malaysia, laos, china hainan, china yunnan, and the like.
Because the seedlings propagated by the rubber tree seeds can not keep the excellent characters of the parent bodies, the rubber tree clone seedlings are adopted as the propagation and planting materials in production. At present, because the tissue culture propagation technology of the rubber trees is not mature, the clone seedlings of the rubber trees are propagated mainly by means of grafting. The grafting of the rubber tree generally does not need the branch grafting, and adopts the seedling bud grafting and the seedling bud grafting, namely, the bud strips of the excellent variety are collected, and then the bud points are cut and grafted on the seedling or the stock of the seedling.
Because the natural rubber is planted in a wide area, and relates to a plurality of countries and regions, in production and scientific research, particularly in the introduction and popularization of fine varieties, buds are required to be obtained from different places or transported to different places for grafting and propagation, and therefore, the effective storage and transportation of the buds are very critical to the grafting and propagation of the rubber trees. Moreover, the buds for grafting and propagating the rubber trees are generally branches which are still in a semi-lignification stage, are relatively young and tender, and are easy to lose water in the transportation and storage processes, so that the buds are inactivated. In addition, the rubber tree planting areas are tropical areas, the environmental temperature in the bud transportation and storage process is often high, if the bud is directly stored and transported without any pretreatment, branches are often severely dehydrated or browned on the surface after 2-3 days, most of the bud points are inactivated, and the buds cannot be propagated.
At present, no effective method for storing and transporting the rubber tree buds exists, and trans-regional transportation and storage of the rubber tree buds become one of key factors for restricting introduction and popularization of fine varieties and grafting and propagation of nursery stocks in production and scientific research.
Disclosure of Invention
In order to solve the problems, the invention provides the method for storing the rubber tree buds in transportation, which is simple and convenient to operate, the rubber tree latex used for sealing the buds is obtained from local materials, the cost is lower, and the activity of the rubber tree buds in the storage and transportation process can be prolonged to 5-6 days.
The invention is realized by the following technical scheme: a method for preserving rubber tree buds suitable for transportation comprises the following steps:
1) cutting off the rubber tree buds, cutting off tender tips at the top ends, and uniformly coating the latex seeped from the notches at the two ends;
2) cutting off all the leaves, wherein each cut off leaf stem cut is uniformly coated with the exuded latex once, and the coating is carried out along with the cutting off;
3) dipping latex of the rubber tree with a cotton ball, uniformly coating the latex on the surface of the buds to seal all parts of the buds, and coating the latex for 1-2 times after every 20-40min to ensure that the thickness of the latex layer covered on the surface of the buds is more than 0.2 mm;
4) after waiting for 60-120min, wrapping the buds with transparent adhesive tape when the latex on the surfaces of the buds is not sticky, putting the buds into a plastic bottle which is not easy to deform, and then tightly sealing the bottle mouth;
5) the plastic bottle is placed in a foam box filled with the ice bag, and the foam box is covered tightly and sealed, so that the plastic bottle can be stored and transported.
The maturity of the rubber tree buds in the step 1) is green and semi-lignified, and the length of the buds is 20-25 cm.
The cutting position of the step 2) is 0.5-1cm away from the base of the petiole.
And (4) winding and wrapping 1-2 layers of buds by using the transparent adhesive tape in the step 4).
And (3) placing 3-5 buds in each bottle in the step 4) into a plastic bottle.
The diameter of the bottle body of the plastic bottle in the step 4) is more than 10cm, the length of the bottle body is more than 25cm, and the diameter of the bottle opening is more than 5 cm.
The invention has the beneficial effects that:
1) the used materials are easy to obtain, and the cost is low;
2) the buds are not easy to lose water or be extruded in the process of storage and transportation;
3) the bud grafting method is used for storing and transporting, and after the bud is reached to a destination, the transparent adhesive tape and the latex wrapping layer on the surface of the bud are torn off, so that the bud grafting method can be used for cutting and grafting bud points. The activity of the bud point is still more than 80% after the bud sticks are stored for 5-6 days, and the method is suitable for long-distance transportation.
Detailed Description
The invention will be better understood from the following examples. It should be noted that variations and modifications can be made by those skilled in the art without departing from the principle of the present invention, and these should also be construed as falling within the scope of the present invention.
Example 1
1) Cutting off green semi-lignified rubber tree buds with the length of 22cm, cutting off tender tips at the top ends, and uniformly coating the latex seeped from the cut parts at the two ends;
2) cutting off all the leaves, wherein the cutting-off position is 0.5cm away from the base of the petiole, and the cut of the petiole is uniformly coated with the exuded latex once every cut off leaf and is coated along with the cutting-off;
3) dipping latex of the rubber tree with a cotton ball, uniformly coating the latex on the surface of the buds to seal all parts of the buds, and coating the latex for 2 times after every 30min to ensure that the thickness of the latex layer covered on the surface of the buds is more than 0.2 mm;
4) after waiting for 90min, winding and wrapping 1 layer of buds by using a transparent adhesive tape when the latex on the surfaces of the buds is not sticky, putting into a plastic bottle which is not easy to deform, placing 4 buds in each bottle, wherein the diameter of the bottle body of the plastic bottle is 10cm, the length of the bottle body is 25cm, and the diameter of the bottle mouth is 5cm, and then tightly sealing the bottle mouth;
5) the plastic bottle is placed in a foam box filled with the ice bag, and the foam box is covered tightly and sealed, so that the plastic bottle can be stored and transported.
And (3) tearing off the transparent adhesive tape and the latex coating on the surface of the bud strip after 5 days to reach the destination, wherein the bud strip is not obviously dehydrated and extruded, and the activity of the bud point is still 83% by detection, so that the bud strip can be used for grafting and propagation.
Example 2
1) Cutting off green semi-lignified rubber tree buds with the length of 20-25cm, cutting off tender tips at the top ends, and uniformly coating latex seeped from notches at two ends;
2) cutting off all the leaves, wherein the cutting-off position is 0.5-1cm away from the base of the petiole, and the cut of the petiole is uniformly coated with the exuded latex once every cutting-off piece and is coated along with the cutting-off;
3) dipping latex of the rubber tree with a cotton ball, uniformly coating the latex on the surface of the buds to seal all parts of the buds, and coating the latex for 1-2 times after every 20min to ensure that the thickness of the latex layer covered on the surface of the buds is more than 0.2 mm;
4) after waiting for 60min, winding and wrapping 2 layers of buds by using a transparent adhesive tape when the latex on the surfaces of the buds is not sticky, putting the buds into a plastic bottle which is not easy to deform, placing 5 buds in each bottle, wherein the diameter of the bottle body of the plastic bottle is 12cm, the length of the bottle body is 30cm, and the diameter of the bottle mouth is 8cm, and then tightly sealing the bottle mouth;
5) the plastic bottle is placed in a foam box filled with the ice bag, and the foam box is covered tightly and sealed, so that the plastic bottle can be stored and transported.
And (3) after 5 days, tearing off the transparent adhesive tape and the latex coating on the surface of the bud, wherein the bud is not obviously dehydrated and extruded, and the activity of the bud point is still 85% by detection, so that the bud can be used for grafting and propagation.
Example 3
1) Cutting off green semi-lignified rubber tree buds with the length of 20-25cm, cutting off tender tips at the top ends, and uniformly coating latex seeped from notches at two ends;
2) cutting off all the leaves, wherein the cutting-off position is 0.5-1cm away from the base of the petiole, and the cut of the petiole is uniformly coated with the exuded latex once every cutting-off piece and is coated along with the cutting-off;
3) dipping latex of the rubber tree with a cotton ball, uniformly coating the latex on the surface of the buds to seal all parts of the buds, and coating the latex for 1-2 times after every 40min to ensure that the thickness of the latex layer covered on the surface of the buds is more than 0.2 mm;
4) after waiting for 120min, winding and wrapping 2 layers of buds by using a transparent adhesive tape when the latex on the surfaces of the buds is not sticky, putting into a plastic bottle which is not easy to deform, placing 3 buds in each bottle, wherein the diameter of the bottle body of the plastic bottle is 15cm, the length of the bottle body is 40cm, and the diameter of the bottle mouth is 7cm, and then tightly sealing the bottle mouth;
5) the plastic bottle is placed in a foam box filled with the ice bag, and the foam box is covered tightly and sealed, so that the plastic bottle can be stored and transported.
And 5 days later, the sprouts arrive at the destination, the transparent adhesive tapes and the latex coating layers on the surfaces of the sprouts are torn off, the sprouts are not obviously dehydrated and extruded, and the activity of the sprouts is still 82% by detection, so that the method can be used for grafting and propagation.
Comparative example 1: in the same way as in example 1, the procedure of "applying latex 2 times every 30 min" in step 3) was changed to "applying latex 1 time every 5 min".
Comparative example 2: as in example 1, only the "thickness of the latex layer covering the surface of the sprouts greater than 0.2 mm" in step 3) was eliminated and "the thickness of the latex layer covering the surface of the sprouts was changed to 0.05 mm".
Comparative example 3: in the same way as example 1, only after waiting for 90min in step 4), the bud surface latex is not sticky, namely the buds are wrapped by transparent adhesive tape, and after waiting for 10min, the buds are wrapped by transparent adhesive tape.
Comparative example 4: in the same way as in example 1, only the step 4) "is put into a large non-deformable plastic bottle with a diameter of 10cm, a length of 25cm and a diameter of 5cm, instead of the step" is put into a large non-deformable plastic bottle with a diameter of 10cm, a length of 20 cm and a diameter of 2 cm.
Comparative example 5: in the same manner as in example 1, only the step 4) "large plastic bottle which is not easily deformed" was changed to "large plastic bottle which is easily deformed".
Comparative example 6: as in example 1, only step 5) "place plastic bottle in foam box with ice pack" changed to "place plastic bottle in foam box without ice pack in foam box".
Comparative example 7: in the same manner as in example 1, only the latex was replaced with molten paraffin wax, but the procedure was complicated.
The effect of preservation of the sprouts and the activity of the sprouts 5 days after preservation and transportation in each of the examples and comparative examples are shown in the following table.
Claims (6)
1. A method for preserving rubber tree buds suitable for transportation is characterized by comprising the following steps:
1) cutting off bud strips of the rubber trees with green and semi-lignified maturity, cutting off tender tips at the top ends, and uniformly coating the latex seeped from the notches at the two ends;
2) cutting off all the leaves, and smearing the petiole cut with the exuded latex once every time when one leaf is cut off;
3) dipping latex of the rubber tree with a cotton ball, uniformly coating the latex on the surface of the buds to seal all parts of the buds, and coating the latex for 1-2 times after every 20-40min to ensure that the thickness of the latex layer covered on the surface of the buds is more than 0.2 mm;
4) after waiting for 60-120min, wrapping the buds with transparent adhesive tape when the latex on the surfaces of the buds is not sticky, putting the buds into a plastic bottle which is not easy to deform, and then tightly sealing the bottle mouth;
5) the plastic bottle is placed in a foam box filled with the ice bag, and the foam box is covered tightly and sealed, so that the plastic bottle can be stored and transported.
2. The method for preserving rubber tree sprouts adapted for transportation according to claim 1, wherein: the length of the rubber tree bud strip in the step 1) is 20-25 cm.
3. The method for preserving rubber tree sprouts adapted for transportation according to claim 1, wherein: the cutting position of the step 2) is 0.5-1cm away from the base of the petiole.
4. The method for preserving rubber tree sprouts adapted for transportation according to claim 1, wherein: and (4) winding and wrapping 1-2 layers of buds by using the transparent adhesive tape in the step 4).
5. The method for preserving rubber tree sprouts adapted for transportation according to claim 1, wherein: and (3) placing 3-5 buds in each bottle in the step 4) into a plastic bottle.
6. The method for preserving rubber tree sprouts adapted for transportation according to claim 1, wherein: the diameter of the bottle body of the plastic bottle in the step 4) is more than 10cm, the length of the bottle body is more than 25cm, and the diameter of the bottle opening is more than 5 cm.
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| CN201811457779.4A CN109392895B (en) | 2018-11-30 | 2018-11-30 | Rubber tree bud strip preservation method suitable for transportation |
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| DE1542903A1 (en) * | 1966-01-26 | 1970-04-16 | Roland Huber | Method of preserving Christmas trees |
| US4191555A (en) * | 1978-07-14 | 1980-03-04 | Gaf Corporation | Monaza amides and amines as ethylene inhibitors |
| CN101189965B (en) * | 2006-11-19 | 2011-07-13 | 张掖市林业科学技术推广站 | Scion disposal preservation method |
| CN101189963A (en) * | 2006-11-30 | 2008-06-04 | 天津市世纪东方建筑景观雕塑技术开发中心 | Method for fresh-keeping branches and leaves of arborescent trees plant |
| CN100394850C (en) * | 2006-12-06 | 2008-06-18 | 肖余保 | Rubber tree tapping face coldness-proof coating agent and method for producing same |
| CN101803596A (en) * | 2010-04-13 | 2010-08-18 | 中国普洱茶研究院 | Method for fresh preserving tea plant germplasm tress resources collected at wild field |
| CN103271024B (en) * | 2013-04-11 | 2016-06-08 | 陶育照 | A kind of store method of branch |
| CN103931615B (en) * | 2014-04-02 | 2017-02-15 | 深圳市新华南方生物科技股份有限公司 | Preparation method of jatropha curcas film coating agent |
| CN104012522B (en) * | 2014-05-09 | 2015-12-09 | 新疆林业科学院 | The method of long-term storage Chinese tamarisk kind bar |
| CN104145722B (en) * | 2014-07-29 | 2016-06-29 | 中国农业科学院作物科学研究所 | The Excised Embryos of mulberry resting bud section and grafting rejuvenation method |
| CN106259310A (en) * | 2016-08-04 | 2017-01-04 | 山东省林业科学研究院 | A kind of method quickly processed for Forest Tree Plasm Resources field acquisition Forest tree seed bar |
| CN106305997A (en) * | 2016-08-19 | 2017-01-11 | 铜仁市金农绿色农业科技有限公司 | Preservation method of dendrobium candidum |
| CN107873699B (en) * | 2017-11-27 | 2021-02-12 | 云南省热带作物科学研究所 | Preservation method of rubber leaves |
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