CN109381514B - Scutellaria baicalensis extract, composition thereof and application of scutellaria baicalensis extract in radiation injury protection - Google Patents
Scutellaria baicalensis extract, composition thereof and application of scutellaria baicalensis extract in radiation injury protection Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/539—Scutellaria (skullcap)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Abstract
The invention belongs to the technical field of traditional Chinese medicines, and relates to a scutellaria baicalensis extract, a composition thereof and application of the scutellaria baicalensis extract in radiation injury protection. The scutellaria baicalensis extract is prepared by adopting a method comprising the following steps: (1) Adding ethanol into Scutellariae radix, and ultrasonic extracting for one or more times; (2) filtering the ultrasonic extracting solution each time, and then combining the filtrates; (3) Concentrating and drying the combined filtrate to obtain the scutellaria baicalensis extract. The scutellaria baicalensis extract and the composition thereof have obviously better effect when being used for radiation injury protection.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicines, and relates to a scutellaria baicalensis extract, a composition thereof and application of the scutellaria baicalensis extract in radiation injury protection.
Background
With the rapid development of nuclear industry and radiology, the damage of ionizing radiation to human body is more and more emphasized. High doses of radiation cause damage to all systems of the body, with the hematopoietic and immune systems being the most prominent. Ionizing radiation is a non-specific stimulus, and acts on organs, tissues and lymphocytes caused by the body, and finally causes the function of the immune system to be reduced. Since the hematopoietic and immune systems are extremely sensitive to ionizing radiation, it is very necessary to enhance the protection of the hematopoietic and immune systems in radiation protection. The traditional Chinese medicine has certain effect in preventing and treating radiation injury. Through years of research, people find that the traditional Chinese medicines with the effects of clearing heat and removing toxicity, promoting blood circulation to remove blood stasis, enriching blood and benefiting qi, and nourishing yin and increasing white all have radiation resistance effects of different degrees, and the traditional Chinese medicines have wide sources and low toxicity, so that the traditional Chinese medicines show great advantages and potentials in the research of radiation protective agents.
The mechanism of resisting radiation damage of traditional Chinese medicine mainly has the following aspects:
1. scavenging free radicals and resisting oxidation. The radiation causes the water to undergo a radiolysis reaction, producing free radicals, mainly: OH, eaq-, O 2 - 、H 2 O 2 、HO 2 The indirect damage caused by radiation is mainly caused by free radicals generated by water radiolysis, wherein the effect of OH is the most important, and researches prove that 90 percent of DNA damage caused by low-energy-transfer linear density rays is caused by OH. Therefore, free radical scavenging is an important aspect of radiation-resistant research.
2. Protecting the immune system. Immune function change caused by radiation is one of the main manifestations of radiation injury, and immune lymphocytes and organs involved in immune regulation, such as bone marrow, spleen, thymus, etc., are all high-radiosensitive cell populations. The damage of immune system caused by radiation is mainly reflected in the reduction of immune cells and the reduction of immune organ functions, thereby affecting the specific and non-specific immune functions of the body.
3. Protecting the hematopoietic system. Hematopoietic tissues are high-radiosensitive tissues, hematopoietic stem cells, granulocyte progenitor cells and erythroid progenitor cells are main target cells for radiation attack, and radiation often causes damage such as bone marrow suppression, microcirculation disturbance, leukocyte reduction and hematopoietic microenvironment damage.
The flavonoids in scutellaria baicalensis are baicalin, baicalein, wogonin and wogonin which have the activity of removing free radicals due to the structural characteristics. Baicalein and baicalin concentration-dependent scavenging hydroxyl radicals, DPPH radicals and alkyl radicals, while wogonin and wogonoside have weaker scavenging effect on the above radicals. Through three in-vitro tests of free radical scavenging, lipid peroxidation resistance and metal atom reducing capacity, the antioxidant capacity of four flavonoid compounds in scutellaria is compared, baicalein shows consistent antioxidant effect on the three tests, and the baicalein can protect lipid peroxidation; baicalin has a weak antioxidant capacity.
Disclosure of Invention
The invention aims to provide a scutellaria baicalensis extract which has obviously better effect when being used for preventing radiation damage.
To achieve this object, in a basic embodiment, the present invention provides a scutellaria baicalensis extract prepared by a method comprising the steps of:
(1) Adding ethanol into Scutellariae radix, and ultrasonic extracting for one or more times;
(2) Filtering the ultrasonic extracting solution each time, and then combining the filtrates;
(3) Concentrating and drying the combined filtrate to obtain the scutellaria baicalensis extract.
In a preferred embodiment, the present invention provides a scutellaria baicalensis extract, wherein the ethanol concentration in step (1) is 50-70% by volume, and the mass-to-volume ratio of scutellaria baicalensis to ethanol in each ultrasonic extraction is 1.
In a preferred embodiment, the present invention provides a scutellaria baicalensis extract, wherein the temperature of the ultrasonic extraction in the step (1) is 60 to 90 ℃.
In a preferred embodiment, the present invention provides a scutellaria baicalensis extract, wherein the ultrasonic frequency of the ultrasonic extraction in the step (1) is 10 to 30kHz.
In a preferred embodiment, the present invention provides a scutellaria baicalensis extract, wherein the number of ultrasonic extractions in step (1) is 1 to 3.
In a more preferred embodiment, the present invention provides a scutellaria baicalensis extract, wherein the time for each ultrasonic extraction in step (1) is 0.5 to 1.5 hours.
The second purpose of the present invention is to provide a pharmaceutical composition of the scutellaria baicalensis extract, which can have significantly better effect when used for radiation injury protection.
To achieve this object, in a basic embodiment, the present invention provides a pharmaceutical composition of the aforementioned scutellaria baicalensis extract, which comprises an effective radiation damage preventing amount of said scutellaria baicalensis extract, and an appropriate amount of a pharmaceutically acceptable excipient.
In a preferred embodiment, the present invention provides a pharmaceutical composition of the above scutellaria baicalensis extract, wherein the dosage form of the pharmaceutical composition is capsule or granule.
The third purpose of the present invention is to provide a use of the scutellaria baicalensis extract or the pharmaceutical composition thereof for preparing a radiation injury protection medicament, so that the scutellaria baicalensis extract or the pharmaceutical composition thereof can have a significantly better effect when used for radiation injury protection.
To achieve the object, in a basic embodiment, the present invention provides a use of the aforementioned scutellaria baicalensis extract or a pharmaceutical composition thereof for the preparation of a medicament for the protection against radiation damage.
In a preferred embodiment, the present invention provides a use of the above-mentioned scutellaria baicalensis extract or a pharmaceutical composition thereof for the preparation of a medicament for the protection against radiation damage, wherein the radiation damage is 60 Gamma ray radiation damage caused by Co.
The invention has the beneficial effects that the scutellaria baicalensis extract and the pharmaceutical composition thereof have obviously better effect when being used for radiation injury protection.
The scutellaria baicalensis extract and the pharmaceutical composition thereof have the advantages of obvious radiation damage resistance effect, simple and convenient composition, convenient administration, high safety and less adverse reaction, and are easily accepted by patients.
The scutellaria baicalensis extract is pre-gastrically administered to a mouse, and then is proved by a mouse large-dose irradiation survival rate experiment, peripheral blood leukocyte count and spleen and thymus coefficient determination after mouse low-dose irradiation, a mouse water maze experiment and the like: the scutellaria baicalensis extract can remarkably improve the survival rate of mice under the irradiation of large dose; the leukocyte number and spleen and thymus coefficients of the mouse under low-dose irradiation are obviously increased, and the mouse has obvious radiation damage resistance; can obviously improve the cognitive dysfunction of mice under low-dose irradiation. Therefore, the scutellaria baicalensis extract has obvious effect of resisting radiation damage.
Detailed Description
The following examples further illustrate embodiments of the present invention.
Example 1: preparation of Scutellaria baicalensis Georgi extract
The scutellaria baicalensis extract for radiation damage protection is prepared by the following method.
Collecting Scutellariae radix 50g, cutting, adding 60% (v/v) ethanol 60ml per gram of medicinal materials, extracting at 70 deg.C under 20kHz ultrasonic for 2 times, each time for 1 hr, standing, and filtering. Concentrating the filtrate, and drying to obtain Scutellariae radix extract.
Example 2: preparation of Scutellaria baicalensis Georgi extract
The scutellaria baicalensis extract for radiation damage protection is prepared by the following method.
Collecting Scutellariae radix 50g, cutting into pieces, adding 70% (v/v) ethanol 40ml per gram of medicinal material, extracting at 60 deg.C under 10kHz ultrasonic for 3 times (each time for 0.5 hr), standing, and filtering. Concentrating the filtrate, and drying to obtain Scutellariae radix extract.
Example 3: preparation of Scutellaria baicalensis Georgi extract
The scutellaria baicalensis extract for radiation damage protection is prepared by the following method.
Collecting Scutellariae radix 50g, cutting into pieces, adding 50% (v/v) ethanol 70ml per gram of medicinal materials, extracting at 90 deg.C under 30kHz ultrasonic for 1 time for 1.5 hr, standing, and filtering. Concentrating the filtrate, and drying to obtain Scutellariae radix extract.
Example 4: animal experiment for survival rate of large-dose radiation damage protection of scutellaria baicalensis extract
(1) Laboratory apparatus
50mL calibrated centrifuge tubes (Corning Corp.), animal gavage apparatus (6 cm, 12. Sup. St. GmbH technologies, gmbH, beijing).
(2) Laboratory animal
18-22 g of male ICR mice (China institute for food and drug inspection, production license number: SCXK (Jing) 2014-0013).
(3) Experimental medicine
Test drugs: the extracts of scutellaria prepared in the previous examples 1-3.
Positive control drug: amifostine for injection (Dalianmeiluo large pharmaceutical factory, batch number)
53120502)。
(4) Experimental methods
After 1 week of adaptive feeding, the mice were randomly divided into 6 groups of 10 mice, each group was a blank control group (gavage purified water), a model group (gavage purified water), a positive control group (intraperitoneal injection, 400 mg/kg) and an example 1-3 group (gavage dose is 20 mg/kg). Each mouse in the positive control group and the groups of examples 1 to 3 was administered once daily for 14 consecutive days; each mouse in the placebo and model groups was gavaged with purified water (2 ml/100 g) once a day for 14 days. After the final administration or gavage of purified water for 1h, the mice of the other groups except the blank control group are subjected to 60 Co gamma-ray is uniformly irradiated on the whole body at one time, the source skin distance is 80cm, the irradiation dose is 9.0Gy, and the irradiation dose rate is 96.31cGy/min. Irradiation was performed in the department of radiotherapy in hospitals affiliated to the institute of radiation protection, china. Survival was recorded over 30 days for each group of mice. (5) Results of the experiment
Survival rate in 30 days: survival of each group of animals was observed within 30 days after irradiation, and 30-day survival rate, average survival days, and protection index were calculated. Wherein the protection index = ((ab +30 c)/n)/((a ' b ' +30c ')/n '), wherein a, a ' are mean days to live of dead mice of the administration group and the model group, respectively; b. b' is the number of mouse deaths of the administration group and the model group respectively; c. c' is the number of surviving mice in the administration group and the model group for 30 days respectively; n and n' are the total number of mice in the administration group and the model group respectively.
Survival case for 30 days: after the 30-day observation period is finished, the survival rate of the animals in the blank control group is 100 percent, and the average survival days is 30 days; the survival rate of the model group animals is 30 percent, and the average survival days is 13.6 days; the survival rate of the positive control group animals is 70%, the average survival days is 26.6 days, and the protection index is 1.23; the survival rates of the groups of examples 1-3 were 70%, 80%, respectively, the average survival rates were 23.6 days, 27.2 days, and 26.8 days, respectively, and the protection indices were 1.18, 1.28, and 1.24, respectively.
The experimental results show that the scutellaria baicalensis extract can greatly improve the survival rate of mice subjected to high-dose nuclear radiation.
Example 5: animal experiment for measuring small dose radiation damage protective leucocyte count and spleen and thymus gland coefficient of scutellaria baicalensis extract
(1) Laboratory apparatus
A 50mL scale centrifuge tube (Corning Corp.), an animal gavage apparatus (Beijing Gianedel technologies, ltd., no. 12, 6 cm), a three-part full-automatic hemocytometer (Japan optoelectronic Industrial Co., ltd., MEK-6318K type), and a diluent for a blood cell analyzer (Shanghai optoelectronic medical electronics Co., ltd., lot No. 15050283).
(2) Laboratory animal
18-22 g of male ICR mice (China institute for food and drug inspection, production license number: SCXK (Jing) 2014-0013).
(3) Experimental medicine
Test drugs: the extracts of scutellaria prepared in the previous examples 1-3.
Positive control drug: amifostine for injection (Dalianmeiluo large pharmaceutical factory, batch number)
53120502)。
(4) Experimental methods
After 1 week of adaptive feeding, the mice were randomly divided into 6 groups of 10 mice, each group was a blank control group (gavage purified water), a model group (gavage purified water), a positive control group (intraperitoneal injection, 400 mg/kg) and an example 1-3 group (gavage dose is 20 mg/kg). Each mouse in the positive control group and the groups of examples 1 to 3 was administered once daily for 14 consecutive days; each mouse of the blank control group and the model group was gavaged with purified water (2 ml/100 g) once a day for 14 days. After the last administration or gavage of purified water for 1h, the mice of other groups except the blank control group were subjected to 60 Co gamma-ray is irradiated uniformly on the whole body at one time,the source skin distance is 80cm, the irradiation dose is 3.5Gy, and the irradiation dose rate is 96.31cGy/min. Irradiation was performed in the department of radiotherapy in hospitals affiliated to the institute of radiation protection, china. After the irradiation was completed, all mice except the placebo group were continuously dosed or gavaged with purified water for 14 days, and after the last dose or gavaged with purified water for 1 hour, weighed, bled from the orbit, then sacrificed by removing the cervical vertebrae, and the spleen and thymus were removed and weighed.
(5) Results of the experiment
White blood cell counts and spleen and thymus coefficients were determined for each mouse and were subjected to t-test and anova by sps 22.0 and the results were expressed as mean ± standard deviation, see tables 1 and 2, respectively. The experimental results show that:
1) The immune system of the mouse irradiated by the total dose of 3.5Gy is seriously damaged, so that the number of leucocytes is reduced, and the quality of spleen and thymus of immune organs is reduced;
2) The groups of examples 1-3 of the invention can obviously increase the number of leucocytes and the quality of spleen and thymus, and the effect is close to that of positive drugs.
The experimental results show that the scutellaria baicalensis extract has a protective effect on radiation damage of the immune system of a mouse.
TABLE 1ICR mouse leukocyte count results
Note: compared with the model group, P <0.05, P <0.01.
TABLE 2ICR mouse spleen and thymus coefficients determination results
Note: compared with the model group, P <0.05, P <0.01.
Example 6: animal experiment of cognitive function after small dose radiation irradiation of scutellaria baicalensis extract
(1) Laboratory apparatus
50mL calibrated centrifuge tubes (Corning Corp.), animal gavages (6 cm, 12, beijing Gianedear technologies, inc.), and mouse Morris water maze (Chinese academy of medicine, model DMS-2).
(2) Laboratory animal
18-22 g of male ICR mice (China institute for food and drug inspection, production license number: SCXK (Jing) 2014-0013).
(3) Experimental medicine
Test drugs: the extracts of scutellaria prepared in the previous examples 1-3.
Positive control drug: amifostine for injection (Dalianmeiluo Dayao, lot 53120502).
(4) Experimental methods
After 1 week of adaptive feeding, the mice were randomly divided into 6 groups of 10 mice, each group was a blank control group (gavage purified water), a model group (gavage purified water), a positive control group (intraperitoneal injection, 400 mg/kg) and an example 1-3 group (gavage dose is 20 mg/kg). Each mouse in the positive control group and the groups of examples 1 to 3 was administered once daily for 14 consecutive days; each mouse in the placebo and model groups was gavaged with purified water (2 ml/100 g) once a day for 14 days. After the final administration or gavage of purified water for 1h, the mice of the other groups except the blank control group are subjected to 60 Co gamma-ray is uniformly irradiated on the whole body at one time, the source skin distance is 80cm, the irradiation dose is 3.5Gy, and the irradiation dose rate is 96.31cGy/min. Irradiation was performed in the department of radiotherapy in hospitals affiliated to the institute of radiation protection, china. After irradiation, all mice except the placebo group were continuously dosed or gavaged with purified water for 14 days, water maze training was started on day 10 of dosing or gavage purified water, training was performed 4 times a day (positioning voyage test), and the underwater platform was removed on day 14A memory ability test (space search test).
(5) Results of the experiment
Each mouse was trained 4 times a day for localization navigation latency and last day spatial search latency data, and t-test and analysis of variance were performed by sps 22.0, with results expressed as mean ± standard deviation, see tables 3 and 4, respectively. The experimental results show that:
1) After suffering from radiation damage, the mouse has longer positioning navigation latency and space search latency, less times of crossing a platform and significant difference compared with a blank control group;
2) The learning and memory ability of the mice is influenced after the mice suffer from radiation damage, namely cognitive dysfunction is damaged;
3) The groups of examples 1 to 3 of the present invention all improved this symptom significantly, and the effect was close to that of the positive drug.
The experimental results show that the scutellaria baicalensis extract has a protective effect on the radiation damage of the cognitive function of mice.
TABLE 3 results of latency variation in experimental mice
Note: compared to blank group # p<0.05, ## p<0.01, p compared to model group<0.05,**。
p<0.01
Table 4 results of the number of platform crossings of the experimental group of mice
Note: compared to blank group # p<0.05, ## p<0.01, p compared to model group<0.05,**
p<0.01。
The results of examples 4-6 above show that: the scutellaria baicalensis extracts of the ICR mice in the gastric perfusion examples 1-3 can obviously improve the survival rate of the mice under large-dose radiation after repeated administration for 14 days; the scutellaria baicalensis extracts of the embodiments 1-3 can obviously increase the number of leucocytes and the quality of spleen and thymus of mice under small-dose radiation after repeated administration for 28 days, and the effect is close to that of positive drugs. Therefore, the scutellaria baicalensis extract has a protective effect on radiation damage of the immune system of a mouse; the method can obviously shorten the positioning navigation latency and the space search latency of the mouse under the radiation of small dose, obviously increase the times of passing through a platform, and shows that the scutellaria baicalensis extract has a protective effect on the radiation damage of the cognitive function of the mouse.
Example 7: preparation of scutellaria extract capsule
Taking 10g of the scutellaria baicalensis extract prepared in the embodiment 1-3, adding 15g of starch, respectively, uniformly mixing, wetting with an appropriate amount of ethanol, preparing a soft material, sieving with a 30-mesh sieve, granulating, drying at 70-80 ℃, sieving with a 60-mesh sieve, granulating, encapsulating, and respectively preparing into capsules.
Example 8: preparation of scutellaria root extract granules
Taking 10g of the scutellaria baicalensis extract prepared in the embodiment 1-3, adding 30g of sucrose and 12.5g of dextrin respectively, uniformly mixing, wetting with a proper amount of ethanol, preparing a soft material, sieving with a 14-mesh sieve, granulating, drying at 70-80 ℃, sieving with a 60-mesh sieve, granulating, externally packaging, and preparing granules respectively.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is intended to include such modifications and variations. The foregoing examples or embodiments are merely illustrative of the present invention, which may be embodied in other specific forms or in other specific forms without departing from the spirit or essential characteristics thereof. The described embodiments are, therefore, to be considered in all respects as illustrative and not restrictive. The scope of the invention should be indicated by the appended claims, and any changes that are equivalent to the intent and scope of the claims should be construed to be included therein.
Claims (2)
1. Use of Scutellariae radix extract for preparing medicine for preventing radiation injury; the radiation damage is 60 Gamma ray radiation damage caused by Co;
the scutellaria baicalensis extract is prepared by adopting a method comprising the following steps:
(1) Adding ethanol into Scutellariae radix, and ultrasonic extracting for one or more times;
(2) Filtering the ultrasonic extracting solution each time, and then combining the filtrates;
(3) Concentrating and drying the combined filtrate to obtain the scutellaria baicalensis extract,
wherein:
in the step (1), the volume percentage concentration of the ethanol is 50-70%, the mass volume ratio of the scutellaria baicalensis to the ethanol extracted by ultrasonic every time is 1,
the temperature of ultrasonic extraction in the step (1) is 60-90 ℃,
the ultrasonic frequency of the ultrasonic extraction in the step (1) is 10-30kHz,
the ultrasonic extraction frequency in the step (1) is 1-3,
the time of ultrasonic extraction in the step (1) is 0.5-1.5h.
2. Use of the pharmaceutical composition comprising scutellaria baicalensis extract according to claim 1 for the preparation of a medicament for the protection against radiation damage 60 Gamma ray radiation damage caused by Co; the dosage form of the pharmaceutical composition is capsules or granules;
adding 10g of Scutellariae radix extract, respectively adding 15g of starch, mixing, wetting with appropriate amount of ethanol, making into soft mass, sieving with 30 mesh sieve, granulating, drying at 70-80 deg.C, sieving with 60 mesh sieve, grading, making into capsule, and packaging to obtain capsule;
10g of scutellaria baicalensis extract, 30g of sucrose and 12.5g of dextrin are respectively added, the mixture is respectively uniformly mixed, is wetted by proper amount of ethanol, is made into a soft material, is sieved by a 14-mesh sieve for granulation, is dried at 70-80 ℃, is sieved by a 60-mesh sieve for granulation, is externally packaged, and is respectively prepared into granules.
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| CN101816348A (en) * | 2010-04-27 | 2010-09-01 | 吴申龙 | Antiradiation health care tea |
| CN103251721A (en) * | 2013-05-08 | 2013-08-21 | 山西省肿瘤研究所 | Radiation-proof skin-care gel and preparation method thereof |
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| CN101816348A (en) * | 2010-04-27 | 2010-09-01 | 吴申龙 | Antiradiation health care tea |
| CN103251721A (en) * | 2013-05-08 | 2013-08-21 | 山西省肿瘤研究所 | Radiation-proof skin-care gel and preparation method thereof |
| CN104292868A (en) * | 2014-09-09 | 2015-01-21 | 安徽宏博木业有限公司 | X-ray radiation resistant wood fiber and preparation method thereof |
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