CN104623044A - Application of herba houttuyniae in preparation of medicines for preventing and treating ionizing radiation injury - Google Patents

Abstract

The invention relates to the field of medicines, provides application of herba houttuyniae in preparation of medicines for preventing and treating ionizing radiation injury, and discloses an application of herba houttuyniae extract in preparation of medicines for preventing and treating ionizing radiation injury. The herba houttuyniae extract has the effect of radiation protection in cell level and living animal level, and has a protecting effect in a wide dosage range of lethal agent dosage, sub-lethal agent dosage and low-dosage radiation, is stable in quality, low in cost and wide in source, and can be used for preparing medicines in related fields of injury rescue and disease treatment for preventing and treating ionizing radiation injury.

Description

Herba Houttuyniae is preparing the application in prevention and therapy ionization radiation injury medicine

Relate to field

The present invention relates to field of medicaments, be specifically to extract and the application of next effective ingredient in the associated injury treatment caused for the preparation of prevention and therapy ionizing radiation and disease treatment from plant Herba Houttuyniae.

Background technology

Flourish along with global core cause, nuclear technology is in every field extensive uses such as nuclear power station, aerospace industry, national defence and biological medicines, human contact's ionizing radiation also causes the chance damaged to increase, the nuclear war simultaneously brought along with world's nuclear safety situation is nervous and nuclear terrorism secret worry, the protection of the body injury (referred to as radiation damage) that ionizing radiation causes is being subject to increasing attention with treatment.

On the other hand, the sickness rate of malignant tumor and patient populations are in recent years always in the trend continuing to rise, radiotherapy is as one of essential therapeutic arsenals, play a part indispensable, but high dose radiation irradiates the acute radiation injury of tumor surrounding normal tissue and organ and even the whole body that can cause unavoidably, the side reaction that radiation damage brings seriously limits the extensive use of radiotherapy in oncotherapy, also obviously have impact on the quality of life after tumor patient Radiotherapy and treatment.

The radiation damage treatment related drugs of putting on market at present mainly contains: sulfur-containing compound, hormones, cytokine class and Chinese herbal medicine etc., there is respective inherent shortcoming in them: the general side effect of such as sulfur-containing compound larger respectively, amifostine (having another name called amifostine) is the best compound of the protection effect of generally acknowledging at present as the representative of this compounds, it is international managing body pass through first selectively wide spectrum cytoprotective, but half-life extremely short (7 minutes) and expensive (domestic medical market price 400-500 unit /) limit its application, and hormone medicine to the control of radiation damage mainly to bone marrow nucleated cell, hematopoietic stem cell and CFU-GM, the impact of this type of medicine on sexual organ and reproductive system limits widely using of it, the hemopoietic function of bone marrow system injury that radiation causes can be alleviated and give treatment to cytokine class medicine such as interleukin class, colony stimulating factor class medicine, but its Study On The Radioprotective and administration time closely related (in the medical practice for preventing and give treatment to, this type of medicine requires high to medical care detection level and degree of concern), there is obvious proinflammatory effect, and expensive, be difficult to room temperature and preserve, Chinese herbal medicine class radioprotective composition mainly contains phenols, polysaccharide, natural flavonoid, has the features such as active component is indefinite, low toxicity, studies through for many years, so far without listing or similar drugs to be gone on the market.

The present invention relates to the novelty teabag of plant Herba Houttuyniae extract, be called Herba Houttuyniae extract, there is heat-clearing and toxic substances removing, eliminating carbuncle evacuation of pus effect.But up to now, to Herba Houttuyniae for the preparation of prevention and therapy ionization radiation injury treatment and tumour radiotherapy ancillary drug in application there is not been reported.

Summary of the invention

The object of this invention is to provide the novelty teabag of Herba Houttuyniae.

The novelty teabag of Herba Houttuyniae provided by the present invention is: for the preparation of the treatment of prevention and therapy ionization radiation injury and the application of tumour radiotherapy ancillary drug aspect.

Ionization radiation injury of the present invention comprises fatal dose irradiation, sublethal dose and low dose exposure damage, also comprises the radiation damage that tumour radiotherapy produces.

The present invention adopts the existing Herba Houttuyniae injectio in market, also uses the Herba Houttuyniae water extract extracted voluntarily.The invention provides the novelty teabag of Herba Houttuyniae, its type of service, according to clinical needs, corresponding adjuvant can be added, exist with dosage forms such as tablet, pill, capsule, suspension, solution, syrup, injection, solvent, cream, ointment, sprays.

The using dosage of Herba Houttuyniae novelty teabag provided by the present invention 1-10g/Kg body weight (people), but is not limited thereto scope.

Of the present invention is Herba Houttuyniae herb, Herba Houttuyniae root, processed product or its extract be active component.Described extract is water extract or extractive with organic solvent.Organic solvent refers to ethanol or methanol.Wherein, the concentration of described ethanol is 20%-85%v/v, and preferable range is 45%-55%v/v.The concentration of described methanol is 20%-85%v/v, and preferable range is 45%-55%v/v.

The preparation method of described water extract is with Herba Houttuyniae herb, Herba Houttuyniae root at least one for raw material, pulverizes, soaks, get soak, be drying to obtain; Wherein, preferred amount of water is the 1-10 times amount of raw material; Preferably amount of water is the 2-5 times amount of raw material further;

The preparation method of described extractive with organic solvent is with Herba Houttuyniae herb, at least one profit of Herba Houttuyniae root for raw material, pulverizes, adds organic solvent soaking, get soak, be drying to obtain; Wherein, organic solvent particular methanol or ethanol; Preferred alcohol or methanol addition are the 1-10 times amount of raw material; Further preferred alcohol or methanol addition are the 2-5 times amount of raw material.

Objects and advantages of the present invention are by for illustration and explanation by the non-limitative illustration of following preferred embodiments, and these embodiments provide as an example with reference to accompanying drawing.

Accompanying drawing explanation

Fig. 1 Herba Houttuyniae significantly improves C57 Mus fatal dose and irradiates (χ roentgenization: Fig. 1 a, gamma-rays irradiation: natural law of Fig. 1 b) surviving afterwards.

Fig. 2 Herba Houttuyniae significantly improves C57 Mus Sublethal Doses (9Gy gamma-rays) and irradiates latter 30 days survival rates.

Fig. 3 Herba Houttuyniae irradiates the Hemoprotection of the radiation damage caused to low dosage (5Gy gamma-rays).

Fig. 4 Herba Houttuyniae is to the radiate protective action of MCF-7 Human Breast Cancer Cells (cytological map, 1. matched group under microscope; 2. irradiation group; 3. fish raw meat injection 1 μ l/ml culture fluid; 4. irradiation+fish raw meat injection 1 μ l/ml culture fluid).

Detailed description of the invention

Embodiment one

Single fatal dose (13Gy χ ray/gamma-rays) irradiates the radiation damage treatment caused

Herba Houttuyniae injectio, can prepare voluntarily with reference to published patent of invention description or pertinent literature, also can buy in medical market, administering mode is lumbar injection, and dosage is 200mg/Kg, 400mg/Kg body weight (Mus).

χ beam exposure apparatus X-Rad RS-2000 irradiation instrument, voltage 160KV, band radiation energy is 50KeV, close rate 1.01Gy/min.

Gamma-rays irradiation unit ¹³⁷cs irradiation instrument, close rate 0.5Gy/min.

C57BL/6 mice, male and female half and half, body weight 18-20g, quality certification SCXK (capital) 2012-0001, grouping situation is: do not irradiate group, irradiation to normal saline group, irradiation to Herba Houttuyniae group, often organize 10.

Adopt single fatal dose 13Gy total irradiation, dosage regimen is pre-irradiation 30 minutes intraperitoneal administrations, supplements immediately and is administered once, within every 24 hours afterwards, be administered once, continuous 14 days after irradiation.

Testing index: survival natural law.

Experimental result is shown in Fig. 1, and Herba Houttuyniae can irradiate the life span of C57 Mus by significant prolongation fatal dose.

Embodiment two

Sublethal dose (9Gy gamma-rays) irradiates the radiation damage treatment caused

Medicine information, animal information, irradiation devices and parameter, administering mode are identical with embodiment one.

Exposure dose is 9Gy.

Testing index: 30 days survival rates.

Experimental result is shown in Fig. 2, and Herba Houttuyniae can significantly improve the survival rate of Sublethal Doses C57 Mus.

Embodiment three

Low dosage (5Gy gamma-rays) irradiates the Hemoprotection of the radiation damage caused

Medicine information, animal information, irradiation devices and parameter, administering mode are identical with embodiment one.

Exposure dose is 5Gy.

Testing index: body weight, peripheral blood counting, one-sided femur counting, CFU-GM, index and spleen index, thymus index.

Experimental result is shown in Fig. 3, and Herba Houttuyniae significantly can alleviate the animal hemopoietic system damage that low dose exposure causes.

Embodiment four

Herba Houttuyniae is to kinds of tumor cells/Normocellular radiation (gamma-rays) protective effect

Herba Houttuyniae injectio, Kang Hua Medicine stock Co., Ltd of Henan Province, the accurate word Z41021744 of traditional Chinese medicines, lot number 13022801.

Irradiation devices are identical with embodiment one with parameter.

Cell line adopts MCF-7 Breast Cancer Cell, breast carcinoma MDA-MB-231 cell, hepatoma Hep G 2 cells, lung cancer A549 cell, Human Embryonic Kidney HEK 293 cell, people's microvascular endothelial HMEC-1 cell.

Experimentation:

1. cell line and cultivation

Adopt human hepatoma HepG2 cell, typeⅡ pneumocyte, respectively with containing 10%FBS (Gibico, REF10099-141), containing 100 μ g/ml streptomycins and 100U/ml penicillin, DMEM in high glucose (Hyclone, SH30243.01B) and PRMIl640 (Hyclone, SH30809.01B) cultivate, be placed in 37 DEG C, 5%CO ₂constant incubator in routine passage cultivate.

2. drug treating and irradiation

Cell culture, to exponential phase, changes the culture fluid containing variable concentrations medicine, continues cultivation 24 hours, accepts various dose gamma-rays and irradiates.

3. colony formation

After cell irradiation, inoculate different cell quantity by different exposure doses and be inoculated into 60mm culture dish.Cell continuous culture was fixed with methanol after 3 weeks, and add Ji's nurse Sa application liquid and dye 30 minutes, flowing water is cleaned, clone's number of counting cells number >=50.Cloning efficiency and surviving fraction is calculated by cloning efficiency PE=(matched group clone number/experimental group cell number) × 100% and surviving fraction=clone's number/(experimental group cell number × PE).And click models fitting drafting cell survival curve by many targets.

Testing index: IC ₅₀(killing the radiological dose of 50% cell)

Experimental result is shown in Fig. 4 and table 1, and Herba Houttuyniae can improve kinds of tumor cells or Normocellular radiation tolerance dose, has certain radiate protective action.

Table 1 Herba Houttuyniae irradiates IC to kinds of tumor cells/normal cell gamma-rays irradiating gamma-ray ₅₀impact (Gy)

Comprehensive above embodiment result, explanation the invention has the advantages that:

Herba Houttuyniae can play radiate protective action in cellular level and living animal level, and under radiation dose range widely, all has protective effect, can be used for the treatment of prevention and therapy ionization radiation injury and tumour radiotherapy ancillary drug.

Claims (5)

1. Herba Houttuyniae is preparing the application in prevention and therapy ionization radiation injury treatment medicine.

2. application according to claim 1, wherein said ionization radiation injury treatment includes but not limited to that single fatal dose irradiates the acute ionization radiation injury treatment caused.

3. application according to claim 1, wherein said ionization radiation injury treatment includes but not limited to that the hemopoietic system damage that single/repeatedly low dose exposure causes is given treatment to.

4. Herba Houttuyniae is preparing the application in tumour radiotherapy ancillary drug.

5. application according to claim 4, wherein said tumour radiotherapy includes but not limited to breast carcinoma.