CN102552377A - Anti-radiation damage medicine - Google Patents
Anti-radiation damage medicine Download PDFInfo
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- CN102552377A CN102552377A CN2012100048143A CN201210004814A CN102552377A CN 102552377 A CN102552377 A CN 102552377A CN 2012100048143 A CN2012100048143 A CN 2012100048143A CN 201210004814 A CN201210004814 A CN 201210004814A CN 102552377 A CN102552377 A CN 102552377A
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Abstract
The invention relates to an anti-radiation damage medicine, which is prepared by mixing the following active ingredients in percentage by weight: 50-80% of puerariae lobata polysaccharide and 50-50% of ginseng polysaccharide; and the puerariae lobata polysaccharide and the ginseng polysaccharide are extracted from natural plant puerariae lobata and ginseng, which are the natural medicines capable of achieving protection effect in radiation damage. The medicine provided by the invention is capable of enhancing the immunity and antioxidant stress ability of human body as well as protecting the hematopoiesis function of human body, so the medicine is suitable for preventing and treating body damage caused by radiation.
Description
Technical field
The present invention relates to a kind of by the antiradiation injury medicine of natural plant extracts as active component.
Background technology
Science and technology development living environment to people when for people material progress being provided has also caused pollution and destruction to a certain degree.Ionizing radiation is polluted has become the fourth-largest environmental pollution after water, air, noise.The link that the environmental pollution that causes like nuclear explosion, nuclear energy are produced, the leakage of large-scale nuclear accident, the radiotherapy and the radiopharmaceutic application of tumor; The radioactive substance that building and ornament materials contain etc.; The amount of radiation that these situation can cause people to accept increases; And certain radiation dose can cause the function and the morphological change of each system and organ in the body, and the generation that gives rise to diseases is like tumor, leukemia, reduction fertility etc.Tumour patient also can receive radiation injury to a certain degree in radiotherapy; Make the bone marrow depression and the immune major injury of body; Therefore strengthen the radiation protection prevention or alleviate radiation sickness having become problem demanding prompt solution, research prevention or the medicine that alleviates radiation damage are important topics of medicine and pharmacology area research.
The radioprotectant of using clinically at present mainly contains ammonia sulfydryl class, tryptamines compounds etc., but exists narrow, the shortcoming such as toxic and side effects big short with effective time of effective dosage ranges.Therefore from natural plants, seek the effective ingredient that has the radiation protection effect, alleviates radiotherapy side effect, prevent or alleviate radiation that the harm of health is had the important application meaning.
Flos puerariae lobatae is the flower of legume pueraria lobata Puerariae lobata (Willd.) or Radix Puerariae rattan (Pueraia thomsonii Benth.), is traditional medicine that relieves the effect of alcohol, and modern pharmacological research shows that it has the effect of relieving the effect of alcohol and protecting the liver.Main component in the Flos puerariae lobatae mainly contains osajin (like daidzin, Glycitein, rutin, canopy skin element), saponins (soybean saponin, glycyrrhizin, kaikasaponin) and other compositions (volatile oil, eugenol, paraffin).The Flos puerariae lobatae aboundresources, its former plant all has distribution throughout the country except that Xinjiang, Qinghai and Tibet.
It is traditional rare Chinese medicine that Radix Ginseng (Panax ginseng C.A.Meryer.) belongs to Araliaceae.It can enhance metabolism the modern pharmacology proof, and the conditioning physiological function has remarkable effect in recovery body constitution and aspect keeping fit; There are some researches show that Radix Ginseng has better curative effect to cardiovascular disease, diabetes and tumor.
Summary of the invention
The object of the invention just provides a kind ofly has preventive effect and therapeutical effect and the little natural antiradiation injury medicine of toxic and side effects preferably to radiation damage.
The inventor finds that through a large amount of experimentatioies the Flos puerariae lobatae polysaccharide in the Flos puerariae lobatae has the effect that prevents radiation damage preferably.The present invention mixes the Flos puerariae lobatae polysaccharide with the ginseng polysaccharide in certain proportion, studies its protective effect to radiation damage, and the mixture that the result shows two kinds of polysaccharide is superior to single polysaccharide to the protective effect of radiation damage.
Medicine of the present invention is on the basis of previous research work; Adopt zooperal method; Study the effect of its prevention radiation damage, experimental result shows that the Flos puerariae lobatae polysaccharide has the hemopoietic function of protection body; Effects such as the immunity of enhancing body and anti-oxidation stress ability are applicable to the radiation-induced body injury of prevention.
Antiradiation injury medicine of the present invention; Form by following blended by weight percentage active component: Flos puerariae lobatae polysaccharide 50%～80%; The ginseng polysaccharide 50%～20%, and described Flos puerariae lobatae polysaccharide and ginseng polysaccharide extract by conventional extracting technique of Chinese medicine from natural plants Flos puerariae lobatae and Radix Ginseng.
The method for preparing of medicine of the present invention is following:
1. the extraction of Flos puerariae lobatae polysaccharide: exsiccant Flos puerariae lobatae is added 40 times of water, extract three times, extracting temperature is 90 ℃～100 ℃; Each 3 hours, merge extractive liquid, was concentrated into certain volume; Add dehydrated alcohol deposition 2 times, sucking filtration gets precipitate, volatilize solvent after; After adopting the albumen in the Sevag method disgorging, drying, grind the Flos puerariae lobatae polysaccharide powder.
2. ginseng polysaccharide's extraction: the people participates in 40 times of water, extracts three times, and extracting temperature is 90 ℃～100 ℃; Each 3 hours, merge extractive liquid, was concentrated into certain volume; Add dehydrated alcohol deposition 2 times, sucking filtration gets precipitate, volatilize solvent after; After adopting the albumen in the Sevag method disgorging, drying, grind ginseng polysaccharide's powder.
3. Flos puerariae lobatae polysaccharide and ginseng polysaccharide's powder are pressed the preset proportion mix homogeneously, promptly be prepared into antiradiation injury medicine of the present invention.
Be aided with any pharmaceutically acceptable carrier or adjuvant with medicine of the present invention as active component and can be made into any dosage form of taking commonly used, like tablet, oral liquid etc.
Medicine of the present invention has the immunity and the anti-oxidation stress ability of enhancing body, and the hemopoietic function of protection body is applicable to the radiation-induced body injury of prevention.Medicine of the present invention has preferably preventive effect and therapeutical effect and toxic and side effects little to radiation damage.
Pharmacodynamics test of the present invention is following:
(1) receives of the protective effect of reagent thing to x roentgenization mouse hemopoietic system
1. experiment material
Leukocyte diluent (2% glacial acetic acid), Giemsa dye liquor, methanol, DMEM, calf serum, cell counting count board, inverted microscope.
2. animal divides into groups and handles
Healthy Male Kunming strain mice is provided by Jilin University's Experimental Animal Center.Body weight (20 ± 2) g, routine feeding are divided into 3 groups after 5 days at random, 10 every group.(1) normal control group: do not shine, replace medicine with distilled water; (2) irradiation control group: irradiation replaces medicine with distilled water; (3) low dosage administration group: do not shine gastric infusion every day (300mg/kg.bw.), successive administration 14d; (4) low dose exposure group: irradiation, gastric infusion every day (300mg/kg.bw.), successive administration 14d; (5) high dose administration group: do not shine gastric infusion every day (600mg/kg.bw.), successive administration 14d; (6) high-dose irradiation group: irradiation, gastric infusion every day (600mg/kg.bw.), successive administration 14d.Last carries out the whole body uniform irradiation in order to X ray one time to mice after irritating stomach, and close rate is 1.0Gy/min, and voltage 180kV, electric current 18mA, filter plate are 0.5mm Cu+1.0mm Al, and irradiation source is apart from pinwheel vertical dimension 50cm, and accumulated dose is 5.0Gy.
3. experimental procedure
Mice was shone back 24 hours, and eyeball is got blood, got 10 μ l and added in the 0.19ml leukocyte diluent, counting murine interleukin number.
Eyeball of mouse takes off neck execution after getting blood, cuts the abdominal cavity open and gets liver, spleen (subsequent use), cuts the thoracic cavity open and takes out thymus (subsequent use) and breastbone; Breastbone is wiped clean blood stains, remove muscle, cut off the bone dirt; With mosquito forceps bone marrow being squeezed in dripping has on the microscope slide of a droplet serum push jack.After drying, with the fixing 15min of methanol solution, dry, with freshly prepared Giemsa dye liquor dyeing 10-15min, the flowing water flushing is dried.The microscopically counting.Count 1000 micronucleus numbers of having a liking for to contain in the polychromatocyte.
Get mice one side femur, reject muscle, cut femoral head.The DMEM culture fluid that contains 2% calf serum with the syringe extraction 5mL with No. 7 syringe needles is gone out whole femur bone marrows.And be placed in the bottle that fills the DMEM culture fluid, and filter repeatedly and process single cell suspension.Get above-mentioned cell suspension 10 μ L and add the 0.19mL cell diluent, mix, leave standstill 2～3min, add cell counting count board by conventional counting method counting mouse femur bone marrow nucleated cell number.
Mouse femur bone marrow nucleated cell number is (individual/mL)=four big lattice TCS * 5 * 10
4
Get the opposite side femur, reject muscle, lunge the femur two ends, cut the greater trochanter of femur of DF then with No. 7 syringe needles; Go out medullary cell with 5ml PBS buffer, after the piping and druming, centrifugal 10 minutes of 2000rpm; After the abandoning supernatant, will precipitate piping and druming after, add the HClO of 2mmol/L
45ml, 90 ℃ of hatching hydrolysis 15 minutes, be cooled to room temperature after, centrifugal 15 minutes of 2000rpm gets supernatant, with ultraviolet spectrophotometer at the 268nm place survey absorbance (A).DNA(μg)＝40×50×A。
4. experimental result
Receive the experimental result of reagent thing, see table 1～table 4 protective effect of x roentgenization mouse hemopoietic system.
(1) receives of leukocytic the influencing of reagent thing, see table 1 x roentgenization mice.
Table 1 receives the influence of reagent thing to x roentgenization murine interleukin number
*P＜0.05 is compared with the normal control group
(2) receive of the influence of reagent thing, see table 2 x roentgenization micronuclei in mice rate.
Table 2 receives the influence of reagent thing to x roentgenization micronuclei in mice rate
*P＜0.05 is compared with the normal control group
(3) receive of the influence of reagent thing, see table 3 x roentgenization mouse femur bone marrow dna content.
Table 3 receives the influence of reagent thing to x roentgenization mouse bone marrow cells dna content
*P＜0.05 is compared with the normal control group
(4) receive of the influence of reagent thing, see table 4 x roentgenization mice nucleated cell number.
The influence that table 4 receives the reagent thing that x roentgenization mouse bone marrow cells nucleated cell is counted content
*P＜0.05 is compared with the normal control group
(2) receive of the protective effect of reagent thing to x roentgenization mouse immune system
1. experiment material
Normal saline, eye scissors, tweezers, electronic balance
2. the mensuration of mouse spleen coefficient and thymus coefficient
Mouse spleen and thymus are cleaned blood stains with normal saline, and filter paper blots, and weighs then, calculates organ index.Organ index=organ weights/the weight of animals.
3. experimental result
Receive the immune protective effect result of reagent thing, see table 5 x roentgenization mice.
Table 5 receives the influence of reagent thing to x roentgenization mice organ coefficient
*P＜0.05 is compared with the normal control group
(3) receive of the influence of reagent thing to x roentgenization mouse anti oxidative stress ability
1. experiment material
SOD, GSH-px, CAT test kit, thiobarbituricacid, trichloroacetic acid, Coomassie brilliant blue, ethanol, phosphoric acid, bovine serum albumin; 754 ultra-violet and visible spectrophotometers
2. experimental technique
(1) preparation of murine liver tissue homogenate and preservation
Mouse liver is clean with normal saline flushing, and filter paper is dried.Process 10% tissue homogenate with normal saline after weighing, it is subsequent use to place 4 ℃ of refrigerators to preserve.
(2) mensuration of the vigor of SOD, GSH-px, CAT in the mouse liver tissue.
Adopt xanthine oxidase to measure SOD vigor in the liver organization, adopt the vigor of dithio dinitrobenzoic acid (DTNB) determination of color GSH-px, adopt ammonium molybdate determination of color CAT vigor.Build up the test kit that biological study provides with Nanjing, operate to specifications.Measure the vigor of SOD, GSH-px, CAT in the mouse liver tissue.
(3) MDA Determination on content in the mouse liver tissue
Adopt the content of thiobarbituricacid (TBA) determination of color MDA.Get 10% 400 μ L of liver tissue homogenate in test tube, (MDA 10nmol/mL) does matched group, in above-mentioned solution, adds 20% trichloroacetic acid 2.5mL respectively to get the standard application liquid of same volume simultaneously; Mixing adds 1% TBA 1.0mL again, mixing, boiling water bath reaction 40 minutes; Be cooled to room temperature with flowing water, centrifugal 10 minutes of 2000rpm draws supernatant, under 532nm under the wavelength; With the distilled water zeroing, the 1cm cuvette is measured absorbance.
(4) mensuration of protein content in the mouse liver tissue
Adopt protein content in the Coomassie brilliant blue colorimetric method for determining mouse liver tissue.Liver tissue homogenate is diluted to 1% tissue homogenate with normal saline.Sample thief 30 μ L add the normal saline of 0.97mL, add the Coomassie brilliant blue solution of 3.0mL again, and mixing left standstill 10 minutes.With the mixed liquor zeroing of the Coomassie brilliant blue of 1.0mL normal saline and 3.0mL, under the 595nm wavelength, the 1cm cuvette is measured absorbance.
3. experimental result
Receive the exercising result of reagent thing, see table 6～table 9 the anti-oxidation stress ability of x roentgenization mice.
Table 6 receives the influence of reagent thing to SOD vigor in the x roentgenization murine liver tissue
*P＜0.05 is compared with the normal control group
Table 7 receives the influence of reagent thing to GSH-px vigor in the x roentgenization murine liver tissue
*P＜0.05 is compared with the normal control group
Table 8 receives the influence of reagent thing to CAT vigor in the x roentgenization murine liver tissue
*P＜0.05 is compared with the normal control group
Table 9 receives the influence of MDA content in the reagent thing x roentgenization murine liver tissue
*P＜0.05 is compared with the normal control group
The specific embodiment
Embodiment 1
The preparation of antiradiation drug of the present invention
(1) extraction of Flos puerariae lobatae polysaccharide: exsiccant Flos puerariae lobatae is added 40 times of water, extract three times, extracting temperature is 90 ℃～100 ℃; Each 3 hours, merge extractive liquid, was concentrated into certain volume; Add dehydrated alcohol deposition 2 times, sucking filtration gets precipitate, volatilize solvent after; After adopting the albumen in the Sevag method disgorging, drying, grind the Flos puerariae lobatae polysaccharide powder.
(2) ginseng polysaccharide's extraction: the people participates in 40 times of water, extracts three times, and extracting temperature is 90 ℃～100 ℃; Each 3 hours, merge extractive liquid, was concentrated into certain volume; Add dehydrated alcohol deposition 2 times, sucking filtration gets precipitate, volatilize solvent after; After adopting the albumen in the Sevag method disgorging, drying, grind ginseng polysaccharide's powder.
(3) will mix by weight percentage through Flos puerariae lobatae polysaccharide and the ginseng polysaccharide that this method for distilling obtains, the Flos puerariae lobatae polysaccharide is 50%～80%, ginseng polysaccharide 50%～20%, is the active ingredient composition of medicine of the present invention.
Can be aided with any pharmaceutically acceptable carrier or adjuvant with medicine of the present invention as the effective active composition and process any dosage form of taking commonly used, like tablet, capsule, oral liquid etc.
The capsular preparation of embodiment 2 antiradiation drugs
With mixing polysaccharide powder 100g and the starch mix homogeneously of embodiment 1 with preparation, cross 120 mesh sieves twice, the capsule of packing into behind the mix homogeneously No. 1, every contains polysaccharide 0.25g, is the capsule formulation antiradiation injury medicine.
The preparation of embodiment 3 antiradiation drug tablets
With the mixing polysaccharide 100g of embodiment 1 preparation, with the appropriate amount of starch mix homogeneously, add 10% starch slurry and process soft material behind mistake 80 mesh sieves.Granulate the back in 70～80 ℃ of dryings with 14 mesh sieves, 12 mesh sieve granulate.After adding dried starch and magnesium stearate mix homogeneously, tabletting, every contains polysaccharide 0.25g, is tablet form antiradiation injury medicine of the present invention.
The preparation of embodiment 4 antiradiation drug oral liquids
Weigh the mixing polysaccharide 100g of embodiment 1 preparation, add the 700mL dissolved in distilled water, add Mel 10g, sucrose 8g, citric acid 3g; Behind the mix homogeneously, adding distil water is diluted to the 1000mL mixing and is prepared into solution, sterilization; Be distributed into 100 bottles, be oral liquid formulation antiradiation injury medicine of the present invention.
Claims (2)
1. antiradiation injury medicine; It is characterized in that processing: Flos puerariae lobatae polysaccharide 50%～80% by following blended by weight percentage active component; The ginseng polysaccharide 50%～20%, and described Flos puerariae lobatae polysaccharide and ginseng polysaccharide extract from natural plants Flos puerariae lobatae and Radix Ginseng.
2. antiradiation drug according to claim 1 is characterized in that being aided with the peroral dosage form that any pharmaceutically acceptable carrier or adjuvant are processed any routine.
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| CN2012100048143A CN102552377A (en) | 2012-01-09 | 2012-01-09 | Anti-radiation damage medicine |
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| CN2012100048143A CN102552377A (en) | 2012-01-09 | 2012-01-09 | Anti-radiation damage medicine |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104031167A (en) * | 2014-06-26 | 2014-09-10 | 河南中烟工业有限责任公司 | Kudzuvine flower polysaccharide, extraction and purification method and application thereof as tobacco humectant |
| CN110959864A (en) * | 2019-12-31 | 2020-04-07 | 军事科学院军事医学研究院环境医学与作业医学研究所 | Application of blueberry extract in preparation of functional food for resisting low-dose X-ray radiation damage |
| CN113244407A (en) * | 2021-05-28 | 2021-08-13 | 中国医学科学院生物医学工程研究所 | Application of antioxidant and hematopoiesis promoter in preparation of medicine for treating acute radiation injury |
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| CN1548144A (en) * | 2003-05-21 | 2004-11-24 | 勇 舒 | Sobering-up prepn and its prepn process |
| CN101637254A (en) * | 2008-07-29 | 2010-02-03 | 傅远顺 | Seaweed selenium herbal tea |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104031167A (en) * | 2014-06-26 | 2014-09-10 | 河南中烟工业有限责任公司 | Kudzuvine flower polysaccharide, extraction and purification method and application thereof as tobacco humectant |
| CN110959864A (en) * | 2019-12-31 | 2020-04-07 | 军事科学院军事医学研究院环境医学与作业医学研究所 | Application of blueberry extract in preparation of functional food for resisting low-dose X-ray radiation damage |
| CN113244407A (en) * | 2021-05-28 | 2021-08-13 | 中国医学科学院生物医学工程研究所 | Application of antioxidant and hematopoiesis promoter in preparation of medicine for treating acute radiation injury |
| CN113244407B (en) * | 2021-05-28 | 2023-10-27 | 中国医学科学院生物医学工程研究所 | Application of antioxidant and hematopoietic accelerator in preparation of medicine for treating acute radiation injury |
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Application publication date: 20120711 |