CN109380060A - The manufacturing technology of the wild manual culture medium of Agricus blazei - Google Patents

Abstract

The present invention manufacturing technology of the manual culture medium of wild Agricus blazei, agricultural technology field are to carry out by following step: one, the preparation of culture medium raw material: 1000 grams of water, 20 grams of glucose, 100 grams of new fresh sweet potatoes, 50 grams of potato, 20 grams of agar；Two, sweet potato and potato are cut into the thin slice of 0.3cm ~ 0.5cm, adds 1000 grams of water to boil, pinch squeezing and filter to obtain juice, juice is further filtered to clarification；Three, glucose, agar is added, being slowly heated to 80 DEG C ~ 100 DEG C becomes mixed liquor；Four, access branch teat glass is bundled, in 60 DEG C ~ 70 DEG C points 1/5 volumes of instillation of mixed liquor；Five, medium sterilization: putting into sterilization tank, slowly increases temperature, 90 DEG C ~ 100 DEG C of sterilising temp whens kept for 20 ~ 30 minutes after be naturally cooling to 60 DEG C ~ 70 DEG C；Six, a bundle of test tube after having sterilized, which takes out while hot to stand this bundle test tube bottom surface and plane at 30-degree angle, to cool down.

Description

The manufacturing technology of the wild manual culture medium of Agricus blazei

Technical field

The present invention relates to a kind of production methods of Agricus blazei culture medium, belong to agricultural technology field.

Background technique

Agricus blazei (also known as Brazilian mushroom) originates in Brazil, Peru.It is a kind of saprophytic bacteria growing in summer and autumn, lives in height In warm, how wet, ventilation environment, have almond flavor, it is crisp in taste.Agricus blazei cap is tender, and stem is crisp, with very good taste, and taste is pure fresh Perfume (or spice), edible value are quite high.New fresh sporophore moisture content 85%-87%;40-45 containing crude protein grams in the every 100 grams of dry products of edible part, 38-45 grams of soluble saccharide, 6-8 grams of crude fibre, 3-4 grams, 5-7 grams of ash content of fat;It include 18 kinds of amino acid in protein composition, 8 kinds of essential amino acids of human body are complete, also contain multivitamin and ergosterol.Mannan contained therein is to inhibition tumour (especially ascites carcinoma), medical hemorrhoid complicated by anal fistula, enhancing energy, anti-angiocardiopathy etc. are all effective in cure.

In recent years, the Agricus blazei to emerge rapidly in Japan, scientific name are Agaricus blazei murrill, some direct selling Dealer is referred to as Brazilian mushroom, and originally its place of production is in the Sao Paulo City countryside mountain area Piedade of southeast Brazil, Japanese's discovery It has anticancer, enhancing body immunity effect, thus researching and developing is Agricus blazei health food.The mystery of Brazilian mushroom is early in 1 O just has found that, since local resident's disease attack rate is extremely low, it is long that the Gu Benlong longevity, which speculates, by Brazilian immigrant's Gu Benlong longevity of Japanese origin Phase eat Brazilian mushroom as a result, therefore by this mushroom Introduced from Japan, be sent to Japan three and retry Yan Chu mycology research institute, ask institute The assistant research fellow of the long rock last of the twelve Earthly Branches studies carefully, and confirms the effect for truly having anticancer, promoting immunity via multinomial experiment later, thus reputation is dry greatly.

Agricus blazei fructification is sturdy, and 5 ~ 11 centimetres of bacteria cover diameter, be just hemispherical, be gradually finally at steamed bun shape it is open and flat, Center of top is flat, and there is the filbert fibrous scale to maroon on surface, and Gai Yuan has the fragment of velum.The bacterial context at cap center is thick Up to 11 millimeters, the bacterial context at edge is thin, and bacterial context white becomes micro orange after injured.Lamella is from life, intensively, 8 ~ 10 millimeters wide, from white Color turn yellowish pink, after become dark brown.Stem is cylindric, middle reality, 4 ~ 14 centimetres long, and 1 ~ 3 centimetre of diameter, upper inferior thick or base portion is swollen Greatly, surface near-white, hand become nearly yellow after touching.More than collarium initially have powdery to silk floss consider to be worth doing shape ramentum, after fall off into it is smooth, It is hollow.Collarium is big, upper, film quality, just white, rear micro- brown, there is the adjunct with brown silk floss bits shape under film.Spore oblong It is extremely oval, without germ pore.Mycelia is without clamp connection.Agricus blazei (also known as Brazilian mushroom) originates in Brazil, Peru.It is a kind of summer The saprophytic bacteria of autumn growth, lives in hot, humid and airy environment, has almond flavor, crisp in taste.Agricus blazei cap is tender, Stem is crisp, with very good taste, the pure fresh perfume (or spice) of taste, and edible value is quite high.New fresh sporophore moisture content 85%-87%;Every 100 grams of edible part Dry product energy, anti-angiocardiopathy etc. are all effective in cure.

Common fresh-keeping method for storage and transportation is: appropriate Agricus blazei being packed into polybag, is advisable with 3-5 kilograms.Agricus blazei mushroom Body moisture is unsuitable excessively high, if moisture is higher, should spread fresh mushroom out, and is dried hydrofuge with fan, keeps mushroom body surface face slightly dry;Or It is put into freezer, is handled 16 ~ 24 hours at 1 DEG C ~ 3 DEG C, pack, can be improved fresh-keeping again after mushroom body excess surface moisture scatters and disappears Effect.When pack, the strong paper of a layer absorbent is put in bag bottom in advance, such as old newspaper is reloaded into mushroom, and puts one again on surface Layer paper;If the Agricus blazei of dress is on the high side, also one layer of paper need to be put at middle part.Sack is finally sealed, and reversely covers one layer of polybag again.It will The Agricus blazei for being fitted into polybag is reloaded into bubble chamber, and every case weight is 10 ~ 25 kilograms.With one bottle of water of plastic bottle, it is put into ice Ice chest is made in cabinet, upper one layer of polybag is covered outside ice chest, and is put between the bag of the mushroom in case.Finally stitched with adhesive sticker seal box, Chest has been bundled, and has transported market sale outside the province in time.

The World Health Organization (WHO) thinks that current all tumor patients 1/3 can prevent completely, there is 1/3 utilization Modern medical service technology can examine in early days completely disconnected, early treatment, there are also 1/3 patients can be subtracted through effectively treating Hypodynia is bitter, improves life quality, extends the survival of patients time.And immunocompetence is improved, nutritional supplementation is carried out in time, exactly effectively One of measure of rehabilitation.Agricus blazei selenium substance rich in and cancerocidal substance, to enhancing human immunity, supplement special procures nutrition There is good effect.

By recognised by people to antitumor effective 15 kinds of medicinal fungis, such as ganoderma lucidum, mushroom, matsutake, Agricus blazei etc. has One common feature, that is, contain anti-tumor activity polysaccharide.Show according to the study: being known as the ganoderma lucidum of ' human world mesona ' Its polysaccharide body content is 1.30g/100g, and the content of Agaricus Blazei Murrill polysaccharide is up to 6.55g/100g, and polyoses content is ganoderma lucidum 5 times.More unique to be, Agricus blazei contains a kind of β (1-6)-D- glucan structure that can quickly improve immunity, This is that ganoderma lucidum is unexistent.Meanwhile Agricus blazei contains β-D- glucan, this peculiar anti-tumor active substance can be direct Promote the activity of phagocyte, macrophage and natural immunity cell.

Summary of the invention

The manufacturing technology of the wild manual culture medium of Agricus blazei solves the problems, such as to adopt the technical scheme that by following step It is rapid to carry out: one, the preparation of culture medium raw material: 1000 grams of water, 20 grams of glucose, 100 grams of new fresh sweet potatoes, 50 grams of potato, agar 20 Gram；Two, sweet potato and potato are cleaned into peeling, is cut into the thin slice of 0.3cm ~ 0.5cm, adds 1000 grams of water to boil 4 ~ 6 minutes, stand drop Temperature is smashed to pieces, is squeezed after being wrapped up with filter cloth with hand, is pinched squeezing and filter to obtain juice, juice is further filtered to clarification, takes upper layer clear Clear liquid；Three, glucose, agar are added in the clarified solution squeezed, is slowly heated to 80 DEG C ~ 100 DEG C, heats while stirring It is even, until sufficiently dissolution becomes mixed liquor；Four, the clean teat glass of access branch is bundled in parallel, is disposed vertically, mixed It is drawn, point is instilled in ten test tubes using dropper when closing 60 DEG C ~ 70 DEG C of liquid, every test tube drips 1/5 volume of loading amount, after the completion of drop Every test tube uses cotton ball stoppered test tube mouth respectively, and cotton ball is unable to contact liq；Five, drop mixing has been filled into medium sterilization: A bundle of test tube of liquid is put into sterilization tank together, slowly increases temperature, and 90 DEG C ~ 100 DEG C of sterilising temp whens are kept for 20 ~ 30 minutes After be naturally cooling to 60 DEG C ~ 70 DEG C；Six, a bundle of test tube after having sterilized takes out, and temperature is not less than 60 DEG C when taking-up, while hot This bundle test tube bottom surface and plane are placed at 30-degree angle, stand liquid level in test tube after being cooled to 15 DEG C ~ 25 DEG C at inclined-plane It is frozen into slant medium.

The beneficial effect of the manufacturing technology of the wild manual culture medium of Agricus blazei is: the wild manual culture medium of Agricus blazei Manufacturing technology uses sweet potato and potato and glucose, agar, water as raw material, by by sweet potato and potato high-temperature maturing, squeezing After filtering to take juice, raw material high temperature mixing, high temperature point is kept to instill test tube, pass through high-temperature sterilization, high temperature test tube slant is placed, and is dropped The shortcomings that temperature sizing inclined-plane, is made mixture slant medium, and pholiota nameko bacterium culture medium can overcome single raw material culture medium, Be conducive to culture propagation fast growing.

Specific embodiment

Embodiment: the manufacturing technology of the wild manual culture medium of Agricus blazei is to implement to carry out by following step: one, water 1000 grams, 20 grams of glucose, 100 grams of new fresh sweet potatoes, 50 grams of potato, 20 grams of agar；Two, sweet potato and potato are cleaned into peeling, cut At the thin slice of 0.3cm ~ 0.5cm, add 1000 grams of water to boil 4 ~ 6 minutes, stand cooling, smash to pieces, is squeezed after being wrapped up with filter cloth with hand It presses, pinch squeezing and filter to obtain juice, juice is further filtered to clarification, takes supernatant liquid；Three, add in the clarified solution squeezed Enter glucose, agar, be slowly heated to 80 DEG C ~ 100 DEG C, heat while stirring uniformly, until sufficiently dissolution becomes mixed liquor； Four, the clean teat glass of access branch is bundled in parallel, is disposed vertically, is inhaled at 60 DEG C ~ 70 DEG C of mixed liquor using dropper It takes, in point ten test tubes of instillation, every test tube drips 1/5 volume of loading amount, and every test tube stoppers examination with cotton ball respectively after the completion of drop Nozzle, cotton ball are unable to contact liq；Five, medium sterilization: a bundle of test tube that drop has filled mixed liquor is put into sterilization tank together In, slowly increase temperature, 90 DEG C ~ 100 DEG C of sterilising temp whens kept for 20 ~ 30 minutes after be naturally cooling to 60 DEG C ~ 70 DEG C；Six, A bundle of test tube after having sterilized takes out, and temperature is not less than 60 DEG C when taking-up, while hot by this bundle test tube bottom surface and plane Cheng Sanshi It spends angle to place, liquid level in test tube is made to stand at inclined-plane and be frozen into slant medium after being cooled to 15 DEG C ~ 25 DEG C.

Claims (1)

1. the manufacturing technology of the wild manual culture medium of Agricus blazei, it is characterized in that being carried out by following step:

One, the preparation of culture medium raw material: 1000 grams of water, 20 grams of glucose, 100 grams of new fresh sweet potatoes, 50 grams of potato, 20 grams of agar；

Two, sweet potato and potato are cleaned into peeling, is cut into the thin slice of 0.3cm ~ 0.5cm, adds 1000 grams of water to boil 4 ~ 6 minutes, stood Cooling, is smashed to pieces, is squeezed after being wrapped up with filter cloth with hand, is pinched squeezing and filter to obtain juice, juice is further filtered to clarification, takes upper layer Clarified solution；

Three, glucose, agar are added in the clarified solution squeezed, is slowly heated to 80 DEG C ~ 100 DEG C, heats while stirring It is even, until sufficiently dissolution becomes mixed liquor；

Four, the clean teat glass of access branch is bundled in parallel, is disposed vertically, dropper is used at 60 DEG C ~ 70 DEG C of mixed liquor It draws, in point ten test tubes of instillation, every test tube drips 1/5 volume of loading amount, and every test tube is stoppered with cotton ball respectively after the completion of drop Test tube mouth, cotton ball are unable to contact liq；

Five, medium sterilization: a bundle of test tube that drop has filled mixed liquor being put into sterilization tank together, slowly increases temperature, is sterilized 60 DEG C ~ 70 DEG C are naturally cooling to after being kept for 20 ~ 30 minutes at 90 DEG C ~ 100 DEG C of temperature；

Six, a bundle of test tube after having sterilized takes out, and temperature is not less than 60 DEG C when taking-up, while hot by this bundle test tube bottom surface and flat Face is placed at 30-degree angle, and liquid level in test tube is made to stand at inclined-plane and be frozen into slant medium after being cooled to 15 DEG C ~ 25 DEG C.