CN109374511A - A kind of optical path adjustment device of the flow cytometer without fluid path situation - Google Patents
A kind of optical path adjustment device of the flow cytometer without fluid path situation Download PDFInfo
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- CN109374511A CN109374511A CN201811177492.6A CN201811177492A CN109374511A CN 109374511 A CN109374511 A CN 109374511A CN 201811177492 A CN201811177492 A CN 201811177492A CN 109374511 A CN109374511 A CN 109374511A
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- 230000003287 optical effect Effects 0.000 title claims abstract description 125
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- 238000007493 shaping process Methods 0.000 claims abstract description 66
- 238000001514 detection method Methods 0.000 claims abstract description 47
- 238000006243 chemical reaction Methods 0.000 claims abstract description 32
- 238000001917 fluorescence detection Methods 0.000 claims abstract description 19
- 238000000605 extraction Methods 0.000 claims abstract description 17
- 230000005855 radiation Effects 0.000 claims abstract description 17
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- 238000005286 illumination Methods 0.000 claims abstract description 6
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- 238000012512 characterization method Methods 0.000 claims description 7
- 239000000284 extract Substances 0.000 claims description 7
- 230000006835 compression Effects 0.000 claims description 5
- 238000007906 compression Methods 0.000 claims description 5
- 230000005611 electricity Effects 0.000 claims description 4
- 238000012544 monitoring process Methods 0.000 claims description 3
- 238000007689 inspection Methods 0.000 claims description 2
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- 238000010586 diagram Methods 0.000 description 12
- 238000000034 method Methods 0.000 description 6
- 230000001678 irradiating effect Effects 0.000 description 4
- 238000001914 filtration Methods 0.000 description 3
- PLXMOAALOJOTIY-FPTXNFDTSA-N Aesculin Natural products OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](O)[C@H]1Oc2cc3C=CC(=O)Oc3cc2O PLXMOAALOJOTIY-FPTXNFDTSA-N 0.000 description 2
- 241000208340 Araliaceae Species 0.000 description 2
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 2
- 235000003140 Panax quinquefolius Nutrition 0.000 description 2
- 235000008434 ginseng Nutrition 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000004088 simulation Methods 0.000 description 2
- 230000003321 amplification Effects 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
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- 239000000571 coke Substances 0.000 description 1
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- 238000004519 manufacturing process Methods 0.000 description 1
- 239000004005 microsphere Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000008054 signal transmission Effects 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
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Abstract
A kind of optical path adjustment device the present invention provides flow cytometer without fluid path situation, described device includes: radiation source, irradiation spot shaping optical path, standard microballoon rotating device, non-forward scattering light beam shaping optical path, non-forward scattering optical detection circuit, forward scattering light and fluorescent light beam shaping optical path, forward scattering optical detection circuit, multicolor fluorescence light splitting optical path and multichannel fluorescence detection circuit, wherein, the radiation source provides excitation light source for fluorescence excitation;The irradiation spot shaping optical path is used for the illumination spot of light beam of light source boil down to certain size;The standard microballoon rotating device simulates individual cells for rotating the disk for being mounted with standard microballoon one by one by irradiation hot spot;The non-forward scattering light beam shaping optical path is for being focused a certain range of non-forward scattering light light beam;The non-forward scattering optical detection circuit is used to carry out photoelectric conversion to non-forward scattering light and realizes parameter extraction to the electric impulse signal of generation.
Description
The application is that application No. is 201610896195.1, applying date 2016.10.14, a kind of entitled streamings
The divisional application of optical path adjustment device and method of the cell instrument without fluid path situation
Technical field
The present invention relates to the adjustment of the optical path of flow cytometer and Microsphere field, especially a kind of light of no fluid path situation
Road adjusts device.
Background technique
In flow cytometer optical path adjustment system, including irradiating the adjustment of hot spot and the light splitting collection of optical excitation signal.
Since the detection zone of microballoon in flow cytometer is located at the point of intersection of irradiation hot spot and liquid flow path direction, and liquid channel system is not
Stablize to will cause microballoon and enter the relative position of detection zone and have differences, swashs so as to cause the irradiation of microballoon in the detection area
Hair degree is not identical, causes forward scattering light, non-forward scattering light and each channel fluorescence difference in signal strength anisotropic big.Subsequent inspection
Slowdown monitoring circuit obtains pulse parameter information inaccuracy.Existing optical adjustment system places one's entire reliance upon high-precision fluid path control system
System, independence are poor.Fluid path control system complex is cumbersome, and the verification method for controlling precision and laminar flow effect has no standard
Index.
A kind of optical path therefore, it is necessary to flow cytometer that can effectively solve the above problem in no fluid path situation adjusts dress
It sets.
Summary of the invention
According to an aspect of the invention, there is provided a kind of optical path adjustment device of flow cytometer without fluid path situation, institute
Stating device includes: radiation source, irradiation spot shaping optical path, standard microballoon rotating device, non-forward scattering light beam shaping light
Road, non-forward scattering optical detection circuit, forward scattering light and fluorescent light beam shaping optical path, forward scattering optical detection circuit, polychrome
Fluorescence spectrophotometer optical path and multichannel fluorescence detection circuit, wherein
The radiation source provides excitation light source for fluorescence excitation;
The irradiation spot shaping optical path is used for the illumination spot of light beam of light source boil down to certain size;
The standard microballoon rotating device is for rotating the disk for being mounted with standard microballoon to which simulation is single thin
Born of the same parents are one by one by irradiation hot spot;
The non-forward scattering light beam shaping optical path is for gathering a certain range of non-forward scattering light light beam
It is burnt;
The non-forward scattering optical detection circuit is used to carry out non-forward scattering light photoelectric conversion and to the electric arteries and veins of generation
It rushes signal and realizes parameter extraction;
The forward scattering light and fluorescent light beam shaping optical path are used for a certain range of forward scattering light and fluorescence light
Beam is focused;
The forward scattering optical detection circuit is used to carry out photoelectric conversion to forward scattering light and believe the electric pulse of generation
Number realize parameter extraction;
The multicolor fluorescence light splitting optical path is corresponding for being separated and being transferred to by the fluorescence signal of each wave-length coverage
Detection circuit, the multichannel fluorescence detection circuit are used to carry out each channel fluorescence signal photoelectric conversion and to the electric pulse of generation
Signal realizes parameter extraction.
Preferably, using the laser of certain power and wavelength as radiation source, and pass through irradiation spot shaping optical path
Compression shaping is carried out to laser transmitting light beam, which moves according to microballoon in standard microballoon rotating device
The spot size compression of two orthogonal directions is realized in direction, while making to move microballoon in hot spot coke by adjusting spot shaping optical path
It is irradiated at point.
Preferably, which includes one and is mounted with the turntable of standard microballoon, turntable driving dress
It sets and a motor motion control circuit, the turntable for being mounted with standard microballoon passes through rotating blade drive and motor motion control
Circuit is connected, and the control to rotary speed is realized by adjusting motor speed, to change microballoon by hot spot irradiation time and phase
The illuminated time interval of adjacent microballoon.
Preferably, the turntable for being mounted with standard microballoon is made of bottom and top layer two parts, wherein having in one layer for putting
The pit for setting standard microballoon, standard microballoon, which is sealed in pit, by the bonding of top layer and bottom prevents microballoon in rotary course
In fall off.The turntable rotary course Plays microballoon for being mounted with standard microballoon passes through irradiation spot area one by one and forms lateral dissipate
Penetrate light, forward scattering light and assorted fluorescence signal.
Preferably, which is placed in non-forward-scattering angle detection zone, to certain angle
Non- forward scattering light in degree range is focused shaping, and the beam Propagation after shaping is electric to non-forward scattering light detection
Road.The non-forward scattering optical detection circuit is realized at the photoelectric conversion of non-forward-scattering signal and the conditioning of electric impulse signal
Reason, and realize that the electric pulse parameter to characterization microballoon characteristic extracts.
Preferably, the forward scattering light and fluorescent light beam shaping optical path are placed in illumination beam direction, to certain angle model
Forward scattering light and multichannel fluorescent light beam in enclosing are focused shaping, and by the beam Propagation after shaping to forward scattering light
Detection circuit and multicolor fluorescence light splitting optical path.The forward scattering optical detection circuit realizes the photoelectric conversion to forward-scattering signal
And the conditioning processing of electric impulse signal, and realize that the electric pulse parameter to characterization microballoon characteristic extracts.
Preferably, which separates multichannel fluorescence signal by a series of Optical devices,
And the assorted fluorescent light beam after separation is transferred to corresponding multichannel fluorescence detection circuit.The multichannel fluorescence detection circuit is real
Now the conditioning of the photoelectric conversion of fluorescence signal and electric impulse signal is handled, and realizes the electric pulse parameter to characterization microballoon characteristic
It extracts.
According to another aspect of the present invention, a kind of optical path adjusting method of the flow cytometer without fluid path situation is provided, is wrapped
Include step:
Irradiation spot shaping optical path carries out the processing such as compressing to the light beam that radiation source generates, and obtains the irradiation of certain size
Hot spot, and adjusting hot spot focal length passes through microballoon from focal point;
Standard microballoon rotating device adjusts the movement velocity of microballoon, changes microballoon detection frequency and microballoon irradiation time, from
And change corresponding light pulse and electrical impulse frequency and duration;
Non- forward scattering light beam shaping optical path carries out the processing such as shaping to non-forward scattering light, and adjustment optical path focal length makes non-
Detector in forward scattering optical detection circuit is in focal position;
Non- forward scattering optical detection circuit realizes the photoelectric conversion to non-forward scattering light, electric pulse conditioning, analog/digital conversion
And the processing such as parameter extraction, identify the starting point and end point of electric pulse;
Forward scattering light and fluorescent light beam shaping optical path carry out the processing such as shaping to forward scattering light and fluorescent light beam, and will
Beam Propagation after focusing is to multicolor fluorescence light splitting optical path;
Forward scattering light and each channel fluorescence light beam are carried out light-splitting processing according to wavelength information by multicolor fluorescence light splitting optical path,
And by the beam Propagation after light splitting to forward scattering optical detection circuit and corresponding multichannel fluorescence detection circuit;
Forward scattering optical detection circuit realizes the photoelectric conversion to forward scattering light, electric pulse conditioning, analog/digital conversion and ginseng
The processing such as number extraction, identify the starting point and end point of electric pulse;
Multichannel fluorescence detection circuit realizes that the photoelectric conversion to each fluorescence, electric pulse improve, analog/digital conversion and parameter mention
It the processing such as takes, identifies the starting point and end point of electric pulse.
It should be appreciated that aforementioned description substantially and subsequent detailed description are exemplary illustration and explanation, it should not
As the limitation to the claimed content of the present invention.
Detailed description of the invention
With reference to the attached drawing of accompanying, the more purposes of the present invention, function and advantage are by the as follows of embodiment through the invention
Description is illustrated, in which:
Fig. 1 is the embodiment schematic diagram of optical path adjustment device of the flow cytometer of the present invention without fluid path situation;
Fig. 2 is that flow cytometer of the invention forms connection schematic diagram.
Fig. 3 a-3c is radiation source shown in Fig. 2 and a kind of exemplary structure schematic diagram for irradiating spot shaping optical path.Wherein
Fig. 3 a shows change in shape of the laser in the hot spot after shaping lens of radiation source sending.Fig. 3 b-3c is shown in Fig. 3 a
Hot spot light distribution schematic diagram is irradiated after shaping.
Fig. 4 a-4b shows an implementation diagram of standard microballoon rotating device and standard microballoon.
Fig. 5 shows a specific optical path example structure figure of schematical non-forward scattering light beam shaping optical path.
Fig. 6 shows the physical circuit module map of non-forward scattering optical detection circuit.
Fig. 7 shows the physical circuit module map of forward scattering optical detection circuit.
Fig. 8 shows the specific optical path example structure figure of schematical multicolor fluorescence light splitting optical path.
Fig. 9 shows the specific optical path example structure figure of another schematical multicolor fluorescence light splitting optical path.
Figure 10 shows a physical circuit module map of multichannel fluorescence detection circuit.
Specific embodiment
Hereinafter, the embodiment of the present invention will be described with reference to the drawings.In the accompanying drawings, identical appended drawing reference represents identical
Or similar component or same or like step.
By reference to exemplary embodiment, the purpose of the present invention and function and the side for realizing these purposes and function
Method will be illustrated.However, the present invention is not limited to exemplary embodiment as disclosed below;Can by different form come
It is realized.The essence of specification is only to aid in those skilled in the relevant arts' Integrated Understanding detail of the invention.
It is described in detail for combination schematic diagram of the present invention, when describing the embodiments of the present invention, for purposes of illustration only, indicating
The sectional view of device architecture can disobey general proportion and make partial enlargement, and the schematic diagram is example, should not be limited herein
The scope of protection of the invention processed.In addition, the three-dimensional space of length, width and depth should be included in actual fabrication.
A kind of optical path adjustment device the present invention provides flow cytometer without fluid path situation, Fig. 1 are that streaming of the present invention is thin
The system block diagram schematic diagram of optical path adjustment device of born of the same parents' instrument without fluid path situation, as shown in Figure 1, described device includes: radiation source
101, irradiate spot shaping optical path 102, standard microballoon rotating device 103, non-forward scattering light beam shaping optical path 104, it is non-before
To scattering optical detection circuit 105, forward scattering light and fluorescent light beam shaping optical path 106, forward scattering optical detection circuit 107, more
Color fluorescence spectrophotometer optical path 108 and multichannel fluorescence detection circuit 109, wherein
The radiation source 101 provides excitation light source for fluorescence excitation;
The irradiation spot shaping optical path 102 is used for the illumination spot of light beam of light source boil down to certain size;
The standard microballoon rotating device 103 is for rotating the disk for being mounted with standard microballoon to which simulation is single
Cell is one by one by irradiation hot spot;
The non-forward scattering light beam shaping optical path 104 is used to carry out a certain range of non-forward scattering light light beam
It focuses;
The non-forward scattering optical detection circuit 105 is used to carry out non-forward scattering light photoelectric conversion and to the electricity of generation
Pulse signal realizes parameter extraction;
The forward scattering light and fluorescent light beam shaping optical path 106 are used for a certain range of forward scattering light and fluorescence
Light beam is focused;
The forward scattering optical detection circuit 107 is used to carry out forward scattering light photoelectric conversion and to the electric pulse of generation
Signal realizes parameter extraction;
The multicolor fluorescence light splitting optical path 108 is corresponding for being separated and being transferred to by the fluorescence signal of each wave-length coverage
Detection circuit;
The multichannel fluorescence detection circuit 109 is used to carry out each channel fluorescence signal photoelectric conversion and to the electricity of generation
Pulse signal realizes parameter extraction.
Fig. 2 is that a better embodiment of optical path adjustment device of the flow cytometer of the present invention without fluid path situation is illustrated
Figure.Which schematically illustrates radiation source 101, standard microballoon rotating device 103, non-forward scattering light beam shaping optical paths
104 and forward scattering light and the several parts of fluorescent light beam shaping optical path 106.
Preferably, using the laser of certain power and wavelength as radiation source 101, and pass through irradiation spot shaping light
Road 102 carries out compression shaping to laser transmitting light beam, and the irradiation spot shaping optical path 102 is according to standard microballoon rotating device
The microballoon direction of motion realizes the spot size compression of two orthogonal directions in 103, while being made by irradiating spot shaping optical path 102
Movement microballoon is irradiated at light spot focus.Fig. 3 a-3c is radiation source shown in Fig. 2 and the one kind for irradiating spot shaping optical path
Exemplary structure schematic diagram.Wherein Fig. 3 a shows shape of the laser in the hot spot after shaping lens of the sending of radiation source 101
Shape variation.Fig. 3 b-3c is irradiation hot spot light distribution schematic diagram after shaping shown in Fig. 3 a.
Preferably, Fig. 4 a-4b shows an implementation diagram of standard microballoon rotating device 103 and standard microballoon.It should
Standard microballoon rotating device 103 includes one and is mounted with the turntable of standard microballoon, a rotating blade drive and a motor fortune
Dynamic control circuit, the turntable for being mounted with standard microballoon are connected with motor motion control circuit by rotating blade drive, are passed through
Control of the motor speed realization to rotary speed is adjusted, so that it is illuminated by hot spot irradiation time and adjacent microballoon to change microballoon
Time interval.
Preferably, the turntable for being mounted with standard microballoon is made of bottom and top layer two parts, wherein having in one layer for putting
The pit for setting standard microballoon, standard microballoon, which is sealed in pit, by the bonding of top layer and bottom prevents microballoon in rotary course
In fall off.The turntable rotary course Plays microballoon for being mounted with standard microballoon passes through irradiation spot area one by one and forms lateral dissipate
Penetrate light, forward scattering light and assorted fluorescence signal.
Fig. 5 shows a specific optical path example structure of schematical non-forward scattering light beam shaping optical path 104
Figure.Preferably, which is placed in non-forward-scattering angle detection zone, to certain angle range
Interior non-forward scattering light is focused shaping, as shown in figure 5, collecting non-forward scattering light by collecting lens, then passes through
After the filtering of barrier filter, then by condenser lens, by the beam Propagation after shaping to non-forward scattering optical detection circuit
105。
Non- forward scattering optical detection circuit 105 realizes the photoelectric conversion of non-forward-scattering signal and the tune of electric impulse signal
Reason processing, and realize that the electric pulse parameter to characterization microballoon characteristic extracts.Fig. 6 shows non-forward scattering optical detection circuit
105 physical circuit module map.As shown in fig. 6, non-forward scattering optical detection circuit 105 includes sensor, for what will be collected
Optical signal is converted into electric signal, the electric impulse signal of sensor output is amplified, filter etc. handles, and by the electricity after conditioning
Pulse signal transmission is converted into digital signal to subsequent analog-to-digital conversion module, then is input to electric pulse parameter extraction module use
Electric signal parameter needed for extracting.
Preferably, the forward scattering light and fluorescent light beam shaping optical path 106 are placed in illumination beam direction, to certain angle
Forward scattering light and multichannel fluorescent light beam in range are focused shaping, are transferred to forward scattering optical detection circuit 107.It should
Forward scattering optical detection circuit 107, which is realized, handles the photoelectric conversion of forward-scattering signal and the conditioning of electric impulse signal, and
Realize that the electric pulse parameter to characterization microballoon characteristic extracts.
Fig. 7 shows the physical circuit module map of forward scattering optical detection circuit 107.As shown in fig. 7, forward scattering light is examined
Slowdown monitoring circuit 107 include sensor, for the optical signal of collection to be converted into electric signal, to sensor output electric impulse signal into
The processing such as row amplification, filtering, and the electric impulse signal after conditioning is transferred to subsequent analog-to-digital conversion module to be converted into number
Signal, then electric pulse parameter extraction module is input to for extracting required electric signal parameter.
Fig. 8 and Fig. 9 respectively illustrates the specific optical path example structure figure of schematical multicolor fluorescence light splitting optical path 108.
As shown in Figure 8 and Figure 9, forward scattering light is collected by collecting lens, is then divided by multiple dichroism spectroscopes,
After bandpass filter filters, signal multiplication collection is carried out by multiple photomultiplier tubes (PMT1-6).Preferably, the polychrome is glimmering
Light light splitting optical path 108 separates multichannel fluorescence signal by a series of Optical devices, and by the assorted fluorescence after separation
Beam Propagation is to corresponding multichannel fluorescence detection circuit 109.
The multichannel fluorescence detection circuit 109 is realized to the photoelectric conversion of fluorescence signal and the conditioning of electric impulse signal
Reason, and realize that the electric pulse parameter to characterization microballoon characteristic extracts.Figure 10 shows multichannel fluorescence detection circuit 109
One physical circuit module map.As shown in Figure 10, multichannel fluorescence detection circuit 109 includes multichannel fluorescence detection circuit, each
Road includes sensor, for the optical signal of collection to be converted into electric signal, the electric impulse signal of sensor output is amplified,
The processing such as filtering, and the electric impulse signal after conditioning is transferred to subsequent analog-to-digital conversion module to be converted into digital signal, then
Electric pulse parameter extraction module is input to for extracting required electric signal parameter.
Another aspect of the present invention provides a kind of optical path adjusting method of the flow cytometer without fluid path situation, including step
It is rapid:
The light beam that irradiation spot shaping optical path 102 generates radiation source 101 carries out the processing such as compressing, and obtains certain size
Irradiation hot spot, and adjust hot spot focal length make microballoon from focal point pass through;
Standard microballoon rotating device adjusts the movement velocity of microballoon, changes microballoon detection frequency and microballoon irradiation time, from
And change corresponding light pulse and electrical impulse frequency and duration;
Non- forward scattering light beam shaping optical path carries out the processing such as shaping to non-forward scattering light, and adjustment optical path focal length makes non-
Detector in forward scattering optical detection circuit is in focal position;
Non- forward scattering optical detection circuit realizes the photoelectric conversion to non-forward scattering light, electric pulse conditioning, analog/digital conversion
And the processing such as parameter extraction, identify the starting point and end point of electric pulse;
Forward scattering light and fluorescent light beam shaping optical path carry out the processing such as shaping to forward scattering light and fluorescent light beam, and will
Beam Propagation after focusing is to multicolor fluorescence light splitting optical path;
Forward scattering light and each channel fluorescence light beam are carried out light-splitting processing according to wavelength information by multicolor fluorescence light splitting optical path,
And by the beam Propagation after light splitting to forward scattering optical detection circuit and corresponding multichannel fluorescence detection circuit;
Forward scattering optical detection circuit realizes the photoelectric conversion to forward scattering light, electric pulse conditioning, analog/digital conversion and ginseng
The processing such as number extraction, identify the starting point and end point of electric pulse;
Multichannel fluorescence detection circuit realizes that the photoelectric conversion to each fluorescence, electric pulse improve, analog/digital conversion and parameter mention
It the processing such as takes, identifies the starting point and end point of electric pulse.
In conjunction with the explanation and practice of the invention disclosed here, the other embodiment of the present invention is for those skilled in the art
It all will be readily apparent and understand.Illustrate and embodiment is regarded only as being exemplary, true scope of the invention and purport are equal
It is defined in the claims.
Claims (3)
1. a kind of optical path adjustment device of flow cytometer without fluid path situation, which is characterized in that described device includes: irradiation light
Source, irradiation spot shaping optical path, standard microballoon rotating device, non-forward scattering light beam shaping optical path, the inspection of non-forward scattering light
Slowdown monitoring circuit, forward scattering light and fluorescent light beam shaping optical path, forward scattering optical detection circuit, multicolor fluorescence light splitting optical path and multi-pass
Road fluorescence detection circuit, wherein
The radiation source provides excitation light source for fluorescence excitation;
The irradiation spot shaping optical path is used for the illumination spot of light beam of light source boil down to certain size;
The standard microballoon rotating device includes one and is mounted with the turntable of standard microballoon, a rotating blade drive and an electricity
Machine motion control circuit, the turntable for being mounted with standard microballoon are connected by rotating blade drive with motor motion control circuit,
The control to rotary speed is realized by adjusting motor speed, is shone to change microballoon by hot spot irradiation time and adjacent microballoon
The time interval penetrated;
Standard microballoon rotating device is simulated individual cells for rotating the disk for being mounted with standard microballoon and is passed through one by one
Cross irradiation hot spot;
The non-forward scattering light beam shaping optical path is placed in non-forward-scattering angle detection zone, within the scope of certain angle
Non- forward scattering light is focused shaping, and by the beam Propagation after shaping to non-forward scattering optical detection circuit;
Non- forward scattering light beam shaping optical path is for being focused a certain range of non-forward scattering light light beam;
The non-forward scattering optical detection circuit is used to carry out photoelectric conversion to non-forward scattering light and believe the electric pulse of generation
Number realize parameter extraction, realize non-forward-scattering signal photoelectric conversion and electric impulse signal conditioning processing, and realize pair
The electric pulse parameter of characterization microballoon characteristic extracts;
The forward scattering light and fluorescent light beam shaping optical path be used for a certain range of forward scattering light and fluorescent light beam into
Line focusing;
The forward scattering optical detection circuit is used to carry out forward scattering light photoelectric conversion and to the electric impulse signal of generation reality
Existing parameter extraction;
The multicolor fluorescence light splitting optical path by the fluorescence signal of each wave-length coverage for being separated and being transferred to corresponding detection
Circuit, the multichannel fluorescence detection circuit are used to carry out each channel fluorescence signal photoelectric conversion and to the electric impulse signal of generation
Realize parameter extraction.
2. optical path adjustment device according to claim 1, it is characterised in that: made using the laser of certain power and wavelength
For radiation source, and light beam is emitted to laser by irradiation spot shaping optical path and carries out compression shaping, the irradiation spot shaping
Optical path realizes that the spot size of two orthogonal directions is compressed according to the microballoon direction of motion in standard microballoon rotating device, passes through simultaneously
Adjustment spot shaping optical path is irradiated movement microballoon at light spot focus.
3. optical path adjustment device according to claim 1, it is characterised in that: be mounted with the turntable of standard microballoon by bottom and
Top layer two parts composition, wherein there is the pit for placing standard microballoon in one layer, by the bonding of top layer and bottom by standard
Microballoon, which is sealed in pit, prevents microballoon from falling off in rotary course.This is mounted with the turntable rotary course Plays of standard microballoon
Microballoon passes through irradiation spot area one by one and forms side scattered light, forward scattering light and assorted fluorescence signal.
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CN201610896195.1A CN106383082B (en) | 2015-10-14 | 2016-10-14 | A kind of optical path adjustment device and method of the flow cytometer without fluid path situation |
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CN109946219A (en) * | 2019-04-12 | 2019-06-28 | 广西师范大学 | A kind of flow cytometer scattering light and fluorescence detection device and method |
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CN114088606B (en) * | 2021-10-23 | 2023-05-09 | 广州市艾贝泰生物科技有限公司 | Cell analysis device |
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