CN205280574U - Multi -functional optoacoustic, fluorescence is micro - and fluorescence spectra formation of image analytical equipment - Google Patents
Multi -functional optoacoustic, fluorescence is micro - and fluorescence spectra formation of image analytical equipment Download PDFInfo
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- CN205280574U CN205280574U CN201521106505.2U CN201521106505U CN205280574U CN 205280574 U CN205280574 U CN 205280574U CN 201521106505 U CN201521106505 U CN 201521106505U CN 205280574 U CN205280574 U CN 205280574U
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Abstract
The utility model provides a multi -functional optoacoustic, fluorescence is micro - and fluorescence spectra formation of image analytical equipment, the utility model discloses a computer control laser scanning device point by point scanning or three -dimensional accurate electric translation stage point by point scanning, laser pulse energy deposit in the sample, the sample absorbs behind the laser pulse energy thermal energy and produces ultrasonic signal, and ultrasonic signal is by turning into the signal of telecommunication and being amplified by signal amplifier through ultrasonic transducer, supersound signal that the data acquisition system laser scanning device 0 sample sent at every scanning element. Meanwhile, every scanning sample application article of the CCD camera synchronous sampling in the spectrum appearance are the spectral information of fluorescence dorsad, and data acquisition system record spectrum appearance is at the spectral information of every scanning element, and (i) await measuring behind the regional data acquisition on xy (i) plane) last one, and computer program will generate optoacoustic microscopy images, fluorescence microscopy images, and the micro - spectral image of fluorescence of sample to accomplish the sample plane.
Description
Technical field
This utility model relates to a kind of multi-functional optoacoustic, fluorescence microscopy and fluorescence spectrum imaging analysis device, belongs to micro-imaging technique field.
Background technology
Micro-imaging technique is commonly applied to the sample that observation naked eyes cannot be observed, and application includes the fields such as biomedicine, chemistry, physics, metallurgy, measurement, has unique position in the development in a lot of fields. Improving in process at microscopy development, from initial field formula microscope, to confocal microscope afterwards, it has had important breakthrough in amplification, noise resistance etc. But, be no matter traditional field formula microscope, or confocal microscope, be all the light intensity utilizing sample scattering light to imaging of samples, tradition micro-imaging can only obtain the structural images of sample. Even if fluorescence imaging also simply obtains the micro-image of fluorescent light intensity, it is impossible to obtain the spectral signature information of sample; The sample absorption characteristic information to light can not be obtained. Photoacoustic imaging technology is that the deposition utilizing light in biological tissue, absorbing medium produces a kind of imaging technique that the optoacoustic effect of ultrasonic signal grows up, and this imaging technique can reach optical resolution, and resolution is far above ultrasound imaging techniques. Owing to the absorption characteristic of optoacoustic effect Yu sample is closely related, it is possible to obtain the sample characteristic information to the absorption of light, functional imaging technology has potential using value.
Summary of the invention
The purpose of this utility model is to overcome the deficiencies in the prior art, there is provided a kind of multi-functional optoacoustic, fluorescence microscopy and fluorescence spectrum imaging analysis device, it is possible to realize the opto-acoustic microscopic imaging to sample, fluorescent microscopic imaging and fluorescence microscopy light spectrum image-forming simultaneously.
According to the technical scheme that this utility model provides, described multi-functional optoacoustic, fluorescence microscopy and fluorescence spectrum imaging analysis device, is characterized in that: includes three-D electric precision translation stage, laser scanning device, pulsed laser light source, spectrogrph, ultrasonic signal acquisition system and the spectroscopic acquisition system that can move in three directions of x, y and z; Described three-D electric precision translation stage is arranged to place the water box of sample, it is provided with ultrasonic signal window in water cassette bottom portion, the first ultrasonic transducer and the second ultrasonic transducer it is respectively provided with at ultrasonic signal bottom of window and sidepiece, first ultrasonic transducer and the second ultrasonic transducer connect signal amplifier respectively through signal shift switch, and signal amplifier connects ultrasonic signal data collecting system;
At the laser scanning device being provided above making laser move in x, y direction of described three-D electric precision translation stage, between laser scanning device and three-D electric precision translation stage, object lens are set; Heat mirror, beam splitter and pulsed laser light source is set gradually in the side of described laser scanning device; Described spectral signature data acquisition system connects the CCD camera of spectrogrph.
Further, the size of described ultrasonic signal window is more than the size of sample.
Further, hyperacoustic thin film can be passed through in the covering of described ultrasonic signal window.
Further, described ultrasonic signal data collecting system and spectral signature data acquisition system connect the electrical signal of photodetector respectively.
Further, described three-D electric precision translation stage, laser scanning device, pulsed laser light source, ultrasonic signal data collecting system and spectral signature data acquisition system are all connected with computer.
This utility model has the advantages that this utility model is under computer program control, after three-D electric precision translation stage or laser scanning device motion can make laser can focus on and determine location point in x/y plane, computer program controls pulsed laser light source makes it send laser pulse, the laser pulse signal measured is converted into electric pulse and provides for two sets of data acquisition systems and trigger signals by photodetector, and two sets of data acquisition systems gather respectively, ultrasonic signal received by the spectral signal of recording light spectrometer and ultrasonic transducer. After the point by point scanning completing a predeterminable area, the data that computer programs process gathers obtain the optoacoustic micro-image of sample, fluorescence microscope images, fluorescence microscopy spectrum picture. This utility model can obtain forward direction or photoacoustic signal dorsad by signal shift switch. Select to adopt laser scanning device scan pattern or three-dimensional precise electric translation object stage scan pattern (Sample Scan pattern) by computer program, it is thus achieved that meet the scan mode being actually needed.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of imaging analysis device described in the utility model.
Fig. 2 is the signal flow graph of analytical equipment described in the utility model.
Fig. 3 is the schematic diagram of described water box.
Detailed description of the invention
Below in conjunction with concrete accompanying drawing, the utility model is described in further detail.
As shown in FIG. 1 to 3: described multi-functional optoacoustic, fluorescence microscopy and fluorescence spectrum imaging analysis device include computer 1, three-D electric precision translation stage 2, laser scanning device 3, pulsed laser light source 4, beam splitter 5, photodetector 6, ultrasonic signal data collecting system 7, signal amplifier 8, heat mirror 9, spectral signature data acquisition system 10, spectrogrph 11, object lens 12, water box 13, first ultrasonic transducer 14, second ultrasonic transducer 15, signal shift switch 16, ultrasonic signal window 17, thin film 18 etc.
As shown in Figure 1, multi-functional optoacoustic described in the utility model, fluorescence microscopy and fluorescence spectrum imaging analysis device include the three-D electric precision translation stage 2 that can move in three directions of x, y and z, are arranged to place the water box 13 of sample on three-D electric precision translation stage 2; As it is shown on figure 3, be provided with ultrasonic signal window 17 bottom described water box 13, the size of ultrasonic signal window 17 is generally higher than the size of sample, covers at ultrasonic signal window 17 and can pass through hyperacoustic thin film 18; It is respectively provided with, with sidepiece, the first ultrasonic transducer 14 and the second ultrasonic transducer 15 that orientation can be adjusted bottom described ultrasonic signal window 17, described first ultrasonic transducer 14 and the second ultrasonic transducer 15 connect signal amplifier 8 respectively through signal shift switch 16, signal amplifier 8 connects ultrasonic signal data collecting system 7, and the signal output part of ultrasonic signal data collecting system 7 connects computer 1.
At the laser scanning device 3 being provided above making laser at x, y scanning direction of described three-D electric precision translation stage 2, between laser scanning device 3 and three-D electric precision translation stage 2, object lens 12 are set; Heat mirror 9, beam splitter 5 and pulsed laser light source 4 is set gradually in the side of described laser scanning device 3, the pulse laser that pulsed laser light source 4 sends is divided into two bundles through beam splitter 5, a branch of received by photodetector 6, a branch of sample in the water box 13 that heat mirror 9, laser scanning device 3 and object lens 12 arrive on three-D electric precision translation stage 2. The electrical signal of described photodetector 6 connects ultrasonic signal data collecting system 7 and spectral signature data acquisition system 10 respectively. Described spectral signature data acquisition system 10 connects the CCD camera of spectrogrph 11, the spectral information of spectrogrph 11 collected specimens each scanning element fluorescence dorsad.
Above-mentioned three-D electric precision translation stage 2, laser scanning device 3, pulsed laser light source 4, ultrasonic signal data collecting system 7 and spectral signature data acquisition system 10 are all connected with computer 1, computer 1 control respective duty.
Deficiency for tradition microscopic system and ultrasonic imaging technique, in order to obtain the more characteristic information of sample and higher spatial resolution, the fluorescence spectrum signal that this utility model deposits produced ultrasonic signal in the sample by simultaneously recording laser pulse energy and laser excites in the sample, realizes the opto-acoustic microscopic imaging to sample, fluorescent microscopic imaging, fluorescence microscopy light spectrum image-forming simultaneously. This utility model is under computer program control, after three-D electric precision translation stage or laser scanning device motion can make laser can focus on and determine location point in x/y plane, computer program controls pulsed laser light source makes it send laser pulse, the laser pulse signal measured is converted into electric pulse and provides for two sets of data acquisition systems and trigger signals by photodetector, and two sets of data acquisition systems gather respectively, ultrasonic signal received by the spectral signal of recording light spectrometer and ultrasonic transducer. After the point by point scanning completing a predeterminable area, the data that computer programs process gathers obtain the optoacoustic micro-image of sample, fluorescence microscope images, fluorescence microscopy spectrum picture.
Multi-functional optoacoustic described in the utility model, fluorescence microscopy and fluorescence spectrum i mage analysis methods, it is possible to achieve three kinds of functions, have two kinds of scan modes, specifically adopt following steps, as shown in Figure 2:
(1) suitable scan mode is chosen according to actual needs: select laser scanning device 3 to carry out pointwise photoscanning in computer 1 control program or selection three-D electric precision translation stage 2 moves and carries out pointwise Sample Scan;
Select the direction that suitable ultrasonic signal gathers: regulate the first ultrasonic transducer 14 and the second ultrasonic transducer 15 position to the suitable direction in space;
Behind the direction of selected ultrasonic signal acquisition, signal shift switch 16 is switched to corresponding ultrasonic transducer direction (the first ultrasonic transducer 14 direction or the second ultrasonic transducer 15 direction); Regulating sample position makes laser focusing in the region to be measured of sample, accurately regulates the sound field central authorities that the position of corresponding ultrasonic transducer makes laser spot detect at ultrasonic transducer again;
(2) computer 1 programme-control laser scanning device 3 or three-D electric precision translation stage 2 move to precalculated position; Computer 1 sends triggering signal triggering pulsed laser light source 4 and sends laser pulse, and the laser pulse signal received is converted into electric impulse signal Triggered ultrasound signal data acquisition system 7 and spectral signature data acquisition system 10 by photodetector 6; The pulsed laser energy that pulsed laser light source 4 sends deposits in the sample, after sample absorbs pulsed laser energy, thermal expansion produces ultrasonic signal, ultrasonic signal is converted to the signal of telecommunication through ultrasonic transducer and is amplified by signal amplifier, the ultrasonic signal data collecting system 7 each scanning element of acquisition and recording sample the ultrasonic signal sent; Meanwhile, the spectral signal of the CCD camera synchronous acquisition sample each scanning element fluorescence dorsad in spectrogrph 11, spectral signature data acquisition system 10 acquisition and recording spectrogrph 11 is in the fluorescence spectrum information of each scanning element of sample;
(3) under computer 1 programme-control, laser scanning device 3 or three-D electric precision translation stage 2 carry out point by point scanning, repeat the data acquisition of step (2) at each scanning element, complete in region to be measured data collection task a little;
(4) data of all collections are passed to computer 1 by ultrasonic signal data collecting system 7 and spectral signal ultrasound acquisition system 10, and all data of computer 1 routine processes provide that the optoacoustic of sample is micro-, the analysis image of fluorescence microscopy and fluorescence spectrum.
This utility model has the advantage that (1) this utility model realizes gathering produced ultrasonic signal after pulsed laser energy deposits in the sample simultaneously, and sample laser pulse excite lower produced by fluorescence spectrum signal dorsad, process through computer and obtain the optoacoustic micro-image of sample, fluorescence microscope images and fluorescence microscopy spectrum picture; (2) this utility model is by choosing laser scanning device scanning or the scanning of three-D electric precision translation stage, it is possible to realize photoscanning or Sample Scan according to actual needs; (3) this utility model selects different signal sources by signal shift switch, can obtain forward direction or photoacoustic signal dorsad according to actual needs.
Claims (6)
1. a multi-functional optoacoustic, fluorescence microscopy and fluorescence spectrum imaging analysis device, it is characterized in that: including can at x, three-D electric precision translation stage (2) that y and z moves in three directions, laser scanning device (3), pulsed laser light source (4), spectrogrph (11), ultrasonic signal acquisition system (7), spectroscopic acquisition system (10), first ultrasonic transducer (14) and the second ultrasonic transducer (15), three-D electric precision translation stage (2) is arranged to place the water box (13) of sample, first ultrasonic transducer (14) and the second ultrasonic transducer (15) connect signal amplifier (8) respectively through signal shift switch (16), signal amplifier (8) connects ultrasonic signal data collecting system (7), at the laser scanning device (3) being provided above making laser move in x, y direction of described three-D electric precision translation stage (2), object lens (12) are set between laser scanning device (3) and three-D electric precision translation stage (2), heat mirror (9), beam splitter (5) and pulsed laser light source (4) is set gradually in the side of described laser scanning device (3), described spectral signature data acquisition system (10) connects the CCD camera of spectrogrph (11).
2. multi-functional optoacoustic as claimed in claim 1, fluorescence microscopy and fluorescence spectrum imaging analysis device, is characterized in that: the size of described ultrasonic signal window (17) is more than the size of sample.
3. multi-functional optoacoustic as claimed in claim 1, fluorescence microscopy and fluorescence spectrum imaging analysis device, it is characterized in that: be provided with ultrasonic signal window (17) in described water box (13) bottom, cover at ultrasonic signal window (17) and hyperacoustic thin film (18) can be passed through.
4. multi-functional optoacoustic as claimed in claim 1, fluorescence microscopy and fluorescence spectrum imaging analysis device, is characterized in that: described ultrasonic signal data collecting system (7) and spectral signature data acquisition system (10) connect the electrical signal of photodetector (6) respectively.
5. multi-functional optoacoustic as claimed in claim 1, fluorescence microscopy and fluorescence spectrum imaging analysis device, is characterized in that: described three-D electric precision translation stage (2), laser scanning device (3), pulsed laser light source (4), ultrasonic signal data collecting system (7) and spectral signature data acquisition system (10) are all connected with computer (1).
6. multi-functional optoacoustic as claimed in claim 1, fluorescence microscopy and fluorescence spectrum imaging analysis device, is characterized in that: described first ultrasonic transducer (14) and the second ultrasonic transducer (15) are separately positioned on ultrasonic signal window (17) bottom and sidepiece.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201521106505.2U CN205280574U (en) | 2015-12-28 | 2015-12-28 | Multi -functional optoacoustic, fluorescence is micro - and fluorescence spectra formation of image analytical equipment |
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| CN201521106505.2U CN205280574U (en) | 2015-12-28 | 2015-12-28 | Multi -functional optoacoustic, fluorescence is micro - and fluorescence spectra formation of image analytical equipment |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105424606A (en) * | 2015-12-28 | 2016-03-23 | 江南大学 | Multifunctional opto-acoustic, fluorescence microscopic and fluorescence spectrum imaging analytical device and method |
| CN110530797A (en) * | 2019-09-25 | 2019-12-03 | 南京大学 | A kind of the double-mode imaging device and its application method of optoacoustic ghost imaging-fluorescence imaging |
| CN112945851A (en) * | 2021-01-29 | 2021-06-11 | 大连理工大学 | Device capable of reducing external interference and detachably fixing photoacoustic cell |
-
2015
- 2015-12-28 CN CN201521106505.2U patent/CN205280574U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105424606A (en) * | 2015-12-28 | 2016-03-23 | 江南大学 | Multifunctional opto-acoustic, fluorescence microscopic and fluorescence spectrum imaging analytical device and method |
| CN110530797A (en) * | 2019-09-25 | 2019-12-03 | 南京大学 | A kind of the double-mode imaging device and its application method of optoacoustic ghost imaging-fluorescence imaging |
| CN112945851A (en) * | 2021-01-29 | 2021-06-11 | 大连理工大学 | Device capable of reducing external interference and detachably fixing photoacoustic cell |
| CN112945851B (en) * | 2021-01-29 | 2023-10-13 | 大连理工大学 | Device for reducing external interference and detachably fixing photoacoustic cell |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160601 Termination date: 20191228 |