CN111454832B - Cell sorting system and method based on micro-flow control - Google Patents
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Abstract
The invention discloses a cell sorting system and a method based on micro-flow control, wherein the system comprises the following components: the device comprises a microfluidic module, a Raman module, a sorting control module and an optical tweezer module, wherein the optical tweezer module captures cells of a sample injection channel of the microfluidic module, the Raman module collects spectrum signals of the cells to obtain Raman signals, the Raman signals are compared with preset Raman signals, and a first signal or a second signal is generated according to comparison results and is output to the sorting control module; the optical tweezers module releases captured cells according to the first signal or the second signal received by the sorting control module, and the sorting control module inputs the released cells into the second output channel according to the first signal or inputs the released cells into the first output channel according to the second signal. By implementing the invention, the optical tweezers module is only responsible for capturing and releasing cells, and meanwhile, the sorting control module is arranged to realize movement of the cells, so that the complexity of the existing cell sorting device is reduced, and the stability and sorting speed of the system are improved.
Description
Technical Field
The invention relates to the technical field of cell sorting, in particular to a cell sorting system and method based on micro-flow control.
Background
Cell sorting methods commonly used at present are a cell sorting method, a centrifugal sorting method, a laser induced fluorescence sorting method and a magnetic sorting method. These methods utilize the characteristics of different types of cells in terms of size, density, shape, deformability and affinity, and effect the cells by microfluidics to achieve separation. For example, a microstructure sorting system for sorting different kinds of cells according to the characteristics of size, shape and deformability has a very simple principle and operation, but can only sort cells with obvious cell size differences, and has a great limitation. Thus, there is a need for a cell sorting method that is not affected by cell size.
The Raman spectroscopy can measure the spectral fingerprint spectrum of the cell biochemistry, so that the cell can be accurately identified. In theory, the cell types with the same size can be well identified through Raman spectrum, and meanwhile, the type of the cell can be distinguished by combining computer analysis. However, achieving cell sorting requires an additional physical manipulation to spatially separate the target cells from the original promiscuous cells. In the previous research of Raman spectrum sorting cells, the target cells are mostly moved to a special channel or groove for collection by manual control of optical tweezers. However, cell movement based on optical tweezers has high complexity and may lead to inefficient sorting.
Disclosure of Invention
In view of the above, the embodiments of the present invention provide a cell sorting system and method based on micro-fluidic control, so as to solve the technical problem in the prior art that the complexity of moving cells by using optical tweezers is high.
The technical scheme provided by the invention is as follows:
a first aspect of an embodiment of the present invention provides a microfluidic-based cell sorting system, including: the device comprises a microfluidic module, a Raman module, a sorting control module and an optical tweezers module, wherein the optical tweezers module captures cells to be sorted of a sample injection channel of the microfluidic module, the Raman module collects spectrum signals of the captured cells to obtain Raman signals, the Raman signals are compared with preset Raman signals, and a first signal or a second signal is generated according to comparison results and is output to the sorting control module; the optical tweezers module releases captured cells according to a first signal or a second signal received by the sorting control module, and the sorting control module inputs the released cells into a first output channel according to the first signal or inputs the released cells into a second output channel according to the second signal.
Further, the optical tweezers module comprises: the device comprises an optical trap generating module and a beam splitting module, wherein the optical trap generating module is used for generating a light beam for capturing cells, and the beam splitting module is used for splitting the light beam into a plurality of light beams with the same power and inputting the light beams into a plurality of sample injection channels of the microfluidic module.
Further, the optical trap generation module includes: a laser and at least one lens, the laser generated by the laser generating a beam of light through the lens for capturing cells.
Further, the microfluidic-based cell sorting system further comprises: and the reflecting mirror is used for reflecting a plurality of light beams with the same power to a plurality of sample injection channels of the microfluidic module.
Further, the microfluidic-based cell sorting system further comprises: and the beam splitter is used for inputting the light beam for capturing the cells to the sample injection channel of the microfluidic module in a penetrating way and reflecting the spectrum signals of the cells to the Raman module.
Further, the microfluidic module includes: the device comprises a microfluidic chip and a cell sampling and collecting module, wherein the microfluidic chip comprises a plurality of cell sorting channels, and each cell sorting channel comprises a cell sampling channel and two cell output channels; the cell sample introduction and collection module is used for inputting cells to be sorted into the cell sample introduction channels and respectively collecting output cells of the two cell output channels.
Further, the raman module comprises: the spectrometer acquires spectrum signals of captured cells to obtain Raman signals, and outputs the Raman signals to the comparison module; the comparison module compares the Raman signal with a preset Raman signal, generates a first signal or a second signal according to a comparison result, and outputs the first signal or the second signal to the sorting control module.
Further, the sorting control module includes: the pressure controller is used for presetting the corresponding relation between the first signal and the first output channel and the corresponding relation between the second signal and the second output channel; moving the released cells to a first output channel when the pressure controller receives a first signal; when the pressure controller receives the second signal, the released cells are moved to the second output channel.
The second aspect of the embodiment of the invention provides a cell sorting method based on micro-flow control, which comprises the following steps: inputting the cells to be sorted into a sample injection channel of a microfluidic module; capturing cells to be sorted in a sample injection channel according to an optical trap generated by an optical tweezer module; collecting spectrum signals of cells to be sorted to obtain Raman signals; comparing the Raman signal with a preset Raman signal, and judging whether the Raman signal is identical to the preset Raman signal or not; when the Raman signal is the same as the preset Raman signal, the cells to be sorted enter the first output channel under the action of the sorting control module.
Further, the cell sorting method based on the microfluidics further comprises the following steps: and when the Raman signal is different from the preset Raman signal, the cells to be sorted enter the second output channel under the action of the sorting control module.
The technical scheme of the invention has the following advantages:
according to the microfluidic-based cell sorting system and method provided by the embodiment of the invention, the capturing and releasing functions of cells are realized by adopting an optical tweezers technology, the cells to be sorted are identified by combining a Raman technology, meanwhile, the corresponding channels of the required cells are predetermined in the sorting control module, and when the cells captured by the optical tweezers module are determined to be the required cells by adopting the Raman technology, the sorting control module controls the cells to be sorted to enter the corresponding channels of the required cells.
According to the cell sorting system and method based on the micro-flow control, the optical tweezers module is only responsible for capturing and releasing cells to be sorted, and meanwhile, the sorting control module is arranged to realize movement of the cells, so that the complexity of the existing cell sorting device is reduced, and the stability and sorting speed of the system are improved. In addition, the cell sorting system and method based on micro-fluidic provided by the embodiment of the invention can realize sorting of cells with any size by combining the optical tweezers technology and the Raman technology, and expands the application scene of the cell sorting system.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present invention, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.
FIG. 1 is a block diagram of a microfluidic-based cell sorting system in an embodiment of the present invention;
FIG. 2 is a schematic diagram of a cell sorting system based on microfluidics in an embodiment of the invention;
fig. 3 is a schematic structural diagram of a microfluidic chip of a microfluidic-based cell sorting system according to an embodiment of the present invention;
fig. 4 is a flow chart of a microfluidic-based cell sorting method in an embodiment of the invention.
Detailed Description
The following description of the embodiments of the present invention will be made apparent and fully in view of the accompanying drawings, in which some, but not all embodiments of the invention are shown. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
In the description of the present invention, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; the two components can be directly connected or indirectly connected through an intermediate medium, or can be communicated inside the two components, or can be connected wirelessly or in a wired way. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
In addition, the technical features of the different embodiments of the present invention described below may be combined with each other as long as they do not collide with each other.
An embodiment of the present invention provides a microfluidic-based cell sorting system, as shown in fig. 1, including: the device comprises a microfluidic module 10, a Raman module 20, a sorting control module 30 and an optical tweezers module 40, wherein the optical tweezers module 40 captures cells to be sorted of a sample channel of the microfluidic module 10, the Raman module 20 collects spectrum signals of the captured cells to obtain Raman signals, the Raman signals are compared with preset Raman signals, and a first signal or a second signal is generated according to comparison results and is output to the sorting control module 30; the optical tweezers module 40 releases the captured cells according to the first signal or the second signal received by the sorting control module 30, and the sorting control module 30 inputs the released cells into the first output channel according to the first signal or the sorting control module 30 inputs the released cells into the second output channel according to the second signal.
According to the microfluidic-based cell sorting system provided by the embodiment of the invention, the capturing and releasing functions of cells are realized by adopting an optical tweezers technology, the cells to be sorted are identified by combining a Raman technology, meanwhile, the corresponding channels of the required cells are predetermined in the sorting control module, and when the cells captured by the optical tweezers module are determined to be the required cells by adopting the Raman technology, the sorting control module controls the cells to be sorted to enter the corresponding channels of the required cells.
According to the cell sorting system based on the micro-flow control, the optical tweezers module is only responsible for capturing and releasing cells to be sorted, and meanwhile, the sorting control module is arranged to realize movement of the cells, so that the complexity of the existing cell sorting device is reduced, and the stability and sorting speed of the system are improved. In addition, the cell sorting system based on the micro-fluid control provided by the embodiment of the invention can realize sorting of cells with any size by combining an optical tweezers technology and a Raman technology, and expands the application scene of the cell sorting system.
In one embodiment, as shown in fig. 1, the optical tweezer module 40 includes: the optical trap generating module 41 and the beam splitting module 42, the optical trap generating module 41 is used for generating a light beam for capturing cells, and the beam splitting module 42 is used for splitting the light beam into a plurality of light beams with the same power and inputting the light beams into a plurality of sample injection channels of the microfluidic module 10, wherein the light beams can be parallel equidistant light beams in a straight line shape. Optionally, the optical trap generation module 41 includes: a laser and at least one lens, the laser generated by the laser generating a beam of light through the lens for capturing cells. As shown in fig. 2, the optical trap generation module 41 may include a laser 401 and first, second, and third lenses 402, 403, 407.
In particular, optical tweezers are optical potential wells formed by strongly converging laser beams, the basic principle of which is the mechanical effect of momentum transfer between light and particles of a substance. Thus, the optical trap generation module 41 may generate an optical potential trap that captures the cells to be sorted by beam focusing. In addition, when there are multiple sample injection channels, the beam splitting module 42 may be configured to split the light beam output by the optical trap generating module 41 into multiple light beams, and form optical traps in the multiple sample injection channels to capture cells to be sorted. Alternatively, the beam splitting module 42 may include a structure for splitting one beam of light into multiple beams of light, as shown in fig. 2, for example, a beam splitting device 404, a device with a beam splitting function, such as a liquid crystal spatial light modulator or a digital micromirror array, and the like.
In one embodiment, as shown in fig. 2, the microfluidic-based cell sorting system further comprises: and a reflecting mirror 405, configured to reflect the plurality of light beams with the same power to a plurality of sample injection channels of the microfluidic module 10. Specifically, the mirror 405 may be configured to turn the optical path, and when the elements in the cell sorting system need to be placed in a concentrated manner, the mirror 405 may be configured in the system to change the optical path of the light beam transmission.
In one embodiment, as shown in fig. 2, the microfluidic-based cell sorting system further comprises: the beam splitter 406 is configured to transmit the light beam capturing the cell to the sample channel of the microfluidic module 10, and reflect the spectral signal of the cell to the raman module 20.
In one embodiment, as shown in fig. 2, the microfluidic module 10 includes: the micro-fluidic chip 101 and the cell sampling and collecting module 102, wherein the micro-fluidic chip 101 comprises a plurality of cell sorting channels, and each cell sorting channel comprises a cell sampling channel and two cell output channels; the cell sampling and collecting module 102 is used for inputting cells to be sorted into a plurality of cell sampling channels and collecting output cells of the cell output channels respectively.
Specifically, as shown in fig. 3, five cell sorting channels may be disposed in the microfluidic chip 101, where in the sample injection channel, an optical trap area 111 and a control area 112 may be included, and an optical trap generated by the optical trap generating module 41 may be located in the optical trap area 111, and when a cell to be sorted flows into the optical trap area 111, the cell to be sorted may be captured by the optical trap; the force generated by the sorting control module 30 may be in the control region 112 and the cells released by the optical traps may enter the corresponding cell output channels under the force generated by the sorting control module 30 as they move to the control region 112.
In one embodiment, as shown in fig. 2, raman module 20 comprises: the spectrometer 201 and the contrast module 202 are used for acquiring the spectrum signals of the captured cells by the spectrometer 201 to obtain Raman signals, and outputting the Raman signals to the contrast module 202; the comparison module 202 compares the raman signal with a preset raman signal, generates a first signal or a second signal according to the comparison result, and outputs the first signal or the second signal to the sorting control module 30.
Specifically, as the cell to be sorted moves into the optical trap region 111, it is captured by the optical trap, and the cell reflects a corresponding light beam under the action of the light beam generated by the laser 401, and the light beam may be reflected by the beam splitter 406 into the spectrometer 201. The spectrometer 201 may acquire the spectrum signal of the light beam to obtain a raman signal therein and output the raman signal to the contrast module 202. The raman signals of a plurality of cells can be stored in the comparison module 202 in advance, when a certain cell needs to be sorted, the comparison module 202 can compare the acquired raman signal with the raman signal corresponding to the certain cell, and when the acquired raman signal is the same as the raman signal corresponding to the certain cell, the acquired cell to be sorted is the required cell, and at the moment, the comparison module 202 can generate a first signal and output the first signal to the sorting control module 30; when the two are different, it indicates that the cell to be sorted is not the desired cell, and at this time, the comparison module 202 generates a second signal and outputs the second signal to the sorting control module 30. In addition, when the sorting control module 30 receives the first signal or the second signal, the laser 401 may be controlled to be turned off so that the optical trap generated in the optical trap region in the sample channel disappears, and the cells trapped by the optical trap continue to move in the sample channel. Wherein the first signal and the second signal may be 1 or 0, i.e. high and low, respectively. The contrast module 202 may be a microprocessor or the like.
In one embodiment, the sort control module 30 includes: the pressure controller is used for presetting the corresponding relation between the first signal and the first output channel and the corresponding relation between the second signal and the second output channel; when the pressure controller receives the first signal, moving the cells released to the control area to the first output channel; when the pressure controller receives the second signal, the cells released to the control area are moved to the second output channel.
Specifically, the output cell types of the first output channel and the second output channel may be predetermined, for example, the first output channel is determined as the output channel of the desired cell, and the second output channel is determined as the output channel of the other cell. Meanwhile, since the first signal represents that the cell to be sorted is the desired cell, when the pressure controller receives the first signal, the pressure controller can give a force to flow to the first output channel to the cell to be sorted, for example, the first output channel is positioned at the left side of the second output channel, and can give a force to flow to the left side of the cell to be sorted, so that the cell to be sorted flows to the first output channel.
The embodiment of the invention also provides a cell sorting method based on micro-flow control, as shown in fig. 4, which comprises the following steps:
step S101: and inputting the cells to be sorted into a sample injection channel of the microfluidic module.
In an embodiment, the microfluidic module may include a microfluidic chip and a cell sampling and collecting module, the microfluidic chip including a plurality of cell sorting channels, each cell sorting channel including a cell sampling channel and two cell output channels; the cell sample introduction and collection module is used for inputting cells to be sorted into the cell sample introduction channels and respectively collecting output cells of the cell output channels.
Step S102: and capturing the cells to be sorted in the sample injection channel according to an optical trap generated by the optical tweezers module.
In one embodiment, an optical tweezer module includes: the device comprises an optical trap generating module and a beam splitting module, wherein the optical trap generating module is used for generating a light beam for capturing cells, the beam splitting module is used for splitting the light beam into a plurality of light beams with the same power and inputting the light beams into a plurality of sample injection channels of the microfluidic module, and the light beams can be parallel equidistant light beams in a straight line shape. Optionally, the optical trap generation module includes: a laser and at least one lens, the laser generated by the laser generating a beam of light through the lens for capturing cells.
In particular, optical tweezers are optical potential wells formed by strongly converging laser beams, the basic principle of which is the mechanical effect of momentum transfer between light and particles of a substance. Thus, the optical trap generation module may generate an optical potential trap that captures the cells to be sorted by beam focusing. In addition, when a plurality of sample injection channels exist, a beam splitting module can be arranged to split the light beam output by the optical trap generating module into a plurality of light beams, and the light beams respectively form optical traps in the plurality of sample injection channels to capture cells to be sorted. Alternatively, the beam splitting module may include a structure for splitting one beam of light into multiple beams of light, for example, a beam splitting device, or a device having a beam splitting function, such as a liquid crystal spatial light modulator or a digital micromirror array.
In an embodiment, five cell sorting channels may be disposed in the microfluidic chip, where in the sample channel may include an optical trap region, and an optical trap generated by the optical trap generating module may be located in the optical trap region, and when a cell to be sorted flows into the optical trap region, the cell to be sorted may be captured by the optical trap.
Step S103: and collecting the spectrum signals of the cells to be sorted to obtain Raman signals, specifically, after the cells to be sorted are captured, the cells to be sorted reflect corresponding light beams under the action of light beams generated by a laser, and the light beams can be reflected into a spectrometer by a beam splitter. The spectrometer can acquire the spectrum signal of the light beam to obtain a Raman signal therein and output the Raman signal to the contrast module.
Step S104: comparing the Raman signal with a preset Raman signal, and judging whether the Raman signal is identical with the preset Raman signal or not; specifically, the raman signals of a plurality of cells can be stored in the comparison module in advance, and when a certain cell needs to be sorted, the comparison module can compare the acquired raman signal with the raman signal corresponding to the certain cell.
Step S105: when the Raman signal is the same as the preset Raman signal, the cells to be sorted enter the first output channel under the action of the sorting control module. The sample injection channel can further comprise a control area, the force generated by the sorting control module can be located in the control area, and when the cells released by the optical trap move to the control area, the cells can enter the corresponding cell output channel under the action of the force generated by the sorting control module. Specifically, when the raman signal is the same as the preset raman signal, it indicates that the captured cell to be sorted is a desired cell, the comparison module may generate a first signal and output the first signal to the sorting control module, and at this time, the control laser may be controlled to be turned off, so that an optical trap generated in an optical trap area in the sample injection channel disappears, and the cell captured by the optical trap continues to move in the sample injection channel.
In one embodiment, the sorting control module includes: the pressure controller is used for presetting the corresponding relation between the first signal and the first output channel and the corresponding relation between the second signal and the second output channel; when the pressure controller receives the first signal, the cells released to the control area are moved to the first output channel. Wherein the output cell types of the first output channel and the second output channel may be predetermined, for example, the first output channel is determined to be the output channel of the desired cell, and the second output channel is determined to be the output channel of the other cell. Meanwhile, since the first signal represents that the cell to be sorted is the desired cell, when the pressure controller receives the first signal, the pressure controller can give a force to flow to the first output channel to the cell to be sorted, for example, the first output channel is positioned at the left side of the second output channel, and can give a force to flow to the left side of the cell to be sorted, so that the cell to be sorted flows to the first output channel.
Step S106: when the Raman signal is different from the preset Raman signal, the cells to be sorted enter the second output channel under the action of the sorting control module. Specifically, when the raman signal is different from the preset raman signal, it indicates that the cell to be sorted is not the desired cell, and at this time, the comparison module generates a second signal and outputs the second signal to the sorting control module. The sorting control module may, upon receipt of the second signal, impart a force in a right direction to the cells to be sorted, thereby causing the cells to be sorted to flow to the second output channel. Wherein the first signal and the second signal may be 1 or 0, i.e. high and low, respectively.
According to the microfluidic-based cell sorting method provided by the embodiment of the invention, the capturing and releasing functions of cells are realized by adopting an optical tweezers technology, the cells to be sorted are identified by combining a Raman technology, meanwhile, the corresponding channels of the required cells are predetermined in a sorting control module, and when the cells captured by the optical tweezers module are determined to be the required cells by adopting the Raman technology, the sorting control module controls the cells to be sorted to enter the corresponding channels of the required cells.
According to the cell sorting method based on the micro-fluidic control provided by the embodiment of the invention, the optical tweezers module is only responsible for capturing and releasing cells to be sorted, and meanwhile, the sorting control module is arranged to realize movement of the cells, so that the complexity of the existing cell sorting device is reduced, and the stability and sorting speed of the system are improved. In addition, the cell sorting method based on the micro-fluidic system, provided by the embodiment of the invention, can be used for sorting cells with any size by combining an optical tweezers technology and a Raman technology, and expands the application scene of the cell sorting method.
Although the exemplary embodiments and their advantages have been described in detail, those skilled in the art may make various changes, substitutions and alterations to these embodiments without departing from the spirit of the invention and the scope of protection as defined by the appended claims. For other examples, one of ordinary skill in the art will readily appreciate that the order of the process steps may be varied while remaining within the scope of the present invention.
Furthermore, the scope of the present application is not intended to be limited to the particular embodiments of the process, machine, manufacture, composition of matter, means, methods and steps described in the specification. From the present disclosure, it will be readily understood by those of ordinary skill in the art that processes, machines, manufacture, compositions of matter, means, methods, or steps, presently existing or later to be developed that perform substantially the same function or achieve substantially the same result as the corresponding embodiments described herein may be utilized according to the present disclosure. Accordingly, the appended claims are intended to include within their scope such processes, machines, manufacture, compositions of matter, means, methods, or steps.
Claims (8)
1. A microfluidic-based cell sorting system, comprising: a micro-fluidic module, a Raman module, a sorting control module and an optical tweezers module,
the optical tweezers module captures the cells to be sorted of the sample channel of the microfluidic module, the Raman module acquires the spectrum signals of the captured cells to obtain Raman signals, the Raman signals are compared with preset Raman signals, and a first signal or a second signal is generated according to the comparison result and is output to the sorting control module;
the optical tweezers module releases captured cells according to a first signal or a second signal received by the sorting control module, and the sorting control module inputs the released cells into a first output channel according to the first signal or inputs the released cells into a second output channel according to the second signal;
the sorting control module includes: the pressure controller is used for presetting the corresponding relation between the first signal and the first output channel and the corresponding relation between the second signal and the second output channel; moving the released cells to a first output channel when the pressure controller receives a first signal; moving the released cells to a second output channel when the pressure controller receives a second signal;
the optical tweezers module comprises: an optical trap generation module and a beam splitting module,
the optical trap generating module is used for generating a light beam for capturing cells, and the beam splitting module is used for splitting the light beam into a plurality of light beams with the same power and inputting the light beams into a plurality of sample injection channels of the microfluidic module.
2. The microfluidic-based cell sorting system of claim 1, wherein the optical trap generation module comprises: a laser and at least one lens, the laser generated by the laser generating a beam of light through the lens for capturing cells.
3. The microfluidic-based cell sorting system of claim 1, further comprising: and the reflecting mirror is used for reflecting a plurality of light beams with the same power to a plurality of sample injection channels of the microfluidic module.
4. The microfluidic-based cell sorting system of claim 1, further comprising: and the beam splitter is used for inputting the light beam for capturing the cells to the sample injection channel of the microfluidic module in a penetrating way and reflecting the spectrum signals of the cells to the Raman module.
5. The microfluidic-based cell sorting system of claim 1, wherein the microfluidic module comprises: a micro-fluidic chip and a cell sampling and collecting module,
the microfluidic chip comprises a plurality of cell sorting channels, and each cell sorting channel comprises a cell sample injection channel and two cell output channels;
the cell sample introduction and collection module is used for inputting cells to be sorted into the cell sample introduction channels and respectively collecting output cells of the two cell output channels.
6. The microfluidic-based cell sorting system of claim 1, wherein the raman module comprises: a spectrometer and a comparison module, wherein the spectrometer and the comparison module are connected with each other,
the spectrometer acquires a spectrum signal of the captured cell to obtain a Raman signal, and outputs the Raman signal to the comparison module;
the comparison module compares the Raman signal with a preset Raman signal, generates a first signal or a second signal according to a comparison result, and outputs the first signal or the second signal to the sorting control module.
7. A cell sorting method applied to the microfluidic-based cell sorting system according to any one of claims 1 to 6, comprising the steps of:
inputting the cells to be sorted into a sample injection channel of a microfluidic module;
capturing cells to be sorted in a sample injection channel according to an optical trap generated by an optical tweezer module;
collecting spectrum signals of cells to be sorted to obtain Raman signals;
comparing the Raman signal with a preset Raman signal, and judging whether the Raman signal is identical to the preset Raman signal or not;
when the Raman signal is the same as a preset Raman signal, the cells to be sorted enter a first output channel under the action of a sorting control module; the sorting control module includes: the pressure controller is used for presetting the corresponding relation between the first signal and the first output channel and the corresponding relation between the second signal and the second output channel; moving the released cells to a first output channel when the pressure controller receives a first signal; when the pressure controller receives the second signal, the released cells are moved to the second output channel.
8. The microfluidic-based cell sorting method of claim 7, further comprising:
and when the Raman signal is different from the preset Raman signal, the cells to be sorted enter the second output channel under the action of the sorting control module.
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