CN109362788A - A kind of graininess anti-mite agent and its application - Google Patents

A kind of graininess anti-mite agent and its application Download PDF

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Publication number
CN109362788A
CN109362788A CN201811446098.8A CN201811446098A CN109362788A CN 109362788 A CN109362788 A CN 109362788A CN 201811446098 A CN201811446098 A CN 201811446098A CN 109362788 A CN109362788 A CN 109362788A
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acid
mite
graininess
fermentation
mite agent
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CN109362788B (en
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胡文锋
李楚君
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Biological Source Biotechnology (shenzhen) Ltd By Share Ltd
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Biological Source Biotechnology (shenzhen) Ltd By Share Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/10Animals; Substances produced thereby or obtained therefrom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/12Powders or granules
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/02Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/36Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids

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  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Environmental Sciences (AREA)
  • Toxicology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present invention provides a kind of graininess anti-mite agent, and the graininess anti-mite agent is made of the one or more and mentioned microorganism of the microorganism of production formic acid, propionic acid, lactic acid, acetic acid, butyric acid or production valeric acid in the acid that graininess nutrient matrix top fermentation generates.Organic acid contained by the graininess anti-mite agent includes the one or more of formic acid, propionic acid, lactic acid, acetic acid, butyric acid or valeric acid.The graininess nutrient matrix includes the Major Nutrient matrix as fermentation such as granular cereal (paddy, rice, corn, wheat, sorghum, millet etc.) and soybean.Above-mentioned graininess fermentation material has repellent action to acarid.The present invention using particle matrix as thallus and produces the carrier of acid by solid state fermentation, and the moisture in when use absorbable environment continues fermentation and acid, and lasting play is driven mite and acted on.Anti-mite effect of the present invention is good, safe and harmless noresidue;While slow release effective component, fermentation and acid can also continue to, mite effect is driven in enhancing and continuity.

Description

A kind of graininess anti-mite agent and its application
Technical field
The invention belongs to technical field of biological control, more particularly, to a kind of graininess anti-mite agent and its application.
Background technique
Mite class is to belong to Arthropoda Arachnoidea, the small animal of a kind of figure of wide abdomen subclass.Phytophagous mites master There are tetranychid, Panonychus citri, goitre mite, rust mite etc., be a kind of common pest, danger to vegetables, fruit tree, the crops such as cotton often result in Destructive disaster.Parasitism of the acarid in animal is more universal, and almost every kind of animal includes that the life area of the mankind has mite Worm is parasitic.Agriculturally, it is more than 2 months broiler chicken that fowl acarid, which is breeder (including for generations with parent's generation), laying hen and feeding period, Ectoparasite disease that is most common, endangering most serious in (such as Sanhuang chicken, capon) cultivation, is in worldwide distribution, is widely current in Asia The laying hen of the torrid zone and Temperate Region in China.In addition, rabbit itch mite, goat itch mite, the harm of Sarcoptes suis in agricultural production are also very big.Elder brother Worm also has mite evil, as bee mite causes the huge loss of honeybee industry every year;And in black soldier flies cultivation, especially larva cultivates the phase Also see that acarid infects.
The prevention and treatment of mite mainly passes through three kinds of approach, repellent mite, go out mite and blocking mite intrusion.Repellent mite has smell and the sense of taste Point, it is using volatile materials that mite is driven in smell, such as pheromones, the sense of taste drive mite and then applies organic acid etc..More anti-mite agent Using the compound action of two methods.The common drug of chicken mite is Amitraz, and the mechanism of action mainly inhibits single in acarid body The activity of amine oxidase.The prevention and treatment of parasitic mite class generally uses the methods of heat treatment, trapping and chemical prevention, wherein application medicaments Prevention and treatment is the current major measure for controlling parasitic mite class.Mite is prevented and treated in production general with the life habit of host, using aqua It is spraying, smog is stifling, pulvis spreads and controls and mite flutters the methods of lacing film, most commonly seen medicament includes taufluvalinate, fluorochlorobenzene Cyano chrysanthemate and sublimed sulfur drug etc..
Agriculturally important harmful mite due to its breeding cycle it is short, breeding amount is big, and mobility is small, and selfing degree is high, especially holds It is also easy to produce drug resistance.Resistance, which takes place frequently, brings many difficulties to prevention and treatment acarid to people.New acaricide must be continually developed thus. And the increase of acaricide concentration threatens food-safe, also has a negative impact to environment, or even jeopardize food safety. Therefore chemical flooding/acaricidal use must be reduced.However, but promoting the resistance to pharmacopoeia of pest using the insecticide of low concentration Increase;It all kills pest in addition, cannot guarantee that and destroys its growth cycle.To avoid above-mentioned deficiency, need to develop control The safe and non-toxic, harmless of acarid, noresidue, sustainable effective new technology and new product.
Summary of the invention
The present invention is directed to existing issue, provides a kind of graininess anti-mite agent and its application, anti-mite agent provided by the invention Anti-mite effect is good, and drug cost is low, safe and harmless to host.The anti-mite agent is compounded with using what microorganism and its fermentation generated The synergy of machine acid and its confactor plays preventive and therapeutic effect to acarid, can also be after while slow release effective component Continuous to continue post-fermentation production acid using graininess nutrition carrier, humidification effect extends and uses the time.
Above-mentioned technical purpose is implemented with the following technical solutions in the present invention:
A kind of graininess anti-mite agent, the anti-mite agent obtain with the following method: microorganism fungus kind being activated, actication of culture Bacteria concentration is 10 afterwards8~1011Cfu/ml obtains graininess acid fermentation object by solid state fermentation, then low temperature drying to get The anti-mite agent;Wherein, the microbial strains are to produce in formic acid bacterium, bacterium acidi propionici, acetic acid bacteria, lactic acid bacteria or other acid-producing bacterias It is one or more;Preferential, production formic acid bacterium is essential bacterial strain;Other acid-producing bacterias are the strain for producing butyric acid or valeric acid;Graininess acid Property fermentation material total acidity be 20~50g/100g (in terms of lactic acid).
The present invention carries out solid state fermentation with the microorganism fungus kind for producing acid, preferentially using produce the formic acid of the fermentations such as formic acid bacterium as Main active constituent, at the same can other compound strains, co-fermentation, the substances such as organic acid of generation be used as remove acaricidal composition.
Preferably, the fermentation condition of solid state fermentation are as follows: by particulate base soaked overnight, auxiliary material is added, then 120 DEG C of steamings It boils, sterilizes 30 minutes, curing, wherein water content is 60%~70%;To be cooled to 28 DEG C -30 DEG C, by the bacterium after activation Kind access, inoculum concentration 0.1%-1% (v/w);It ferments 24~48 hours under 30~40 DEG C, aerobic or anaerobic condition, fermentation knot Beam, low temperature drying.
The method that the present invention uses solid state fermentation carries out solid state fermentation for example, by using cereal-granules of the present invention, dry in low temperature After dry, the graininess nutrient base material after fermentation is used as the carrier of microbial cell and organic acid simultaneously, uses in external environment When can be absorbed moisture, continue post-fermentation, generate the substance of anti-mite, keep its effect more efficient, persistently.
Preferably, formic acid bacterium such as Wu Shi acetobacter etc. is produced;Bacterium acidi propionici is to produce sour Propionibacterium, propionibacterium freudenreichii, thank One of family name's Propionibacterium, Te Shi Propionibacterium or Beijing Propionibacterium etc. are a variety of;Lactic acid bacteria is lactobacillus acidophilus, cheese One of lactobacillus, Lactococcus lactis or bifidobacterium lactis are a variety of;The clostridium butyricum etc. of butyric acid is produced, but is not limited to above-mentioned bacterium Kind and bacterial strain.Preferably, it selects safe for human and animal and environment, it might even be possible to directly edible microbial strains.
Preferably, the graininess nutrient base material of solid state fermentation is beans or cereal, such as soybean, dregs of beans, paddy, rice, jade One of rice, Gao Liang, wheat or millet etc. are a variety of, and auxiliary material is wheat bran and/or cavings.Wherein, according to the mass fraction, base-material Account for mass fraction 80%~90%;Auxiliary material accounts for mass fraction 10%~20%, and the initial pH for the base-material that ferments is 5.0~7.2.Base-material Fermentation can be advantageously promoted as growth factor supplier directly as solid state fermentation nutrition main provider, auxiliary material.
Due to the method using solid state fermentation, above-mentioned base-material, such as soybean, dregs of beans or corn etc., in solid state fermentation base It is particulate material after control moisture drying, the mode that above-mentioned granular anti-mite agent plays a role is mainly as follows on plinth:
1. above-mentioned graininess anti-mite agent can be released directly due to through everfermentation, thus directly containing the effective component of anti-mite It puts active principle and drives mite anti-mite;
It, after absorbing water can further growth with microorganism therein 2. due to the nutriment that above-mentioned particle itself contains Fermentation generates organic acid, persistently plays anti-mite effect, strengthens and extend anti-mite effect;
The above-mentioned graininess anti-mite agent 3. livestock and poultry can directly search for food, safe and harmless, in excretion, above-mentioned anti-mite agent is through enteron aisle The benefit materials that post-fermentation generates still can contain above-mentioned effective component, thus be conducive to the suppression mite of poultry growing environment and remove Mite.
Preferably, after fermentation, common lower than at 60 DEG C or be dried under vacuum to moisture 10% hereinafter, keeping simultaneously Granular shape.
Anti-mite agent of the present invention livestock and poultry cultivation, pet cultivation, the application of cultured insect environment and grain or The application of mite is driven in feed.
Further, the anti-mite agent is discharged in livestock and poultry, cultured insect environment or pet living environment, every square metre Use 1-10 grams;Or be used in mixed way with grain and feed, the graininess anti-mite agent is 0.1% according to mass concentration ~10% uses.
Further, the process object of graininess anti-mite agent is to colonize in poultry, pet, insect body surface, breeding environment And the harmful mite in cereal, grain and feed.
Graininess anti-mite agent provided by the invention is directly applied easy in livestock and poultry column home ground and surrounding enviroment, such as harmful mite Hiding-place, thick grass, trees gap etc., can more broadly play effect.
Compared with the existing technology, the invention has the following advantages and beneficial effects:
Graininess anti-mite agent provided by the invention is using safe for human and animal and environment, it might even be possible to the edible micro- life of production acid Object fermentation is made, highly-safe, and anti-mite effect is good, and good stability, effect is lasting.Preparation process is easy to operate, and raw material is easy to get, It is easy to spread.Acarid is prevented and treated using beneficial microbe, pesticide residue and the harm to host can be effectively reduced, reduce harmful mite Drug resistance, while reducing control cost and mitigating the pollution to environment.
Detailed description of the invention
Fig. 1 is the Y-shaped smell sensing apparatus experimental provision that the present invention carries out that anti-mite test uses.
Specific embodiment
Further illustrate the present invention below in conjunction with specific embodiments and the drawings, but embodiment the present invention is not done it is any The restriction of form.Unless stated otherwise, the present invention uses reagent, method and apparatus is the art conventional reagents, method And equipment.
Unless stated otherwise, agents useful for same and material of the present invention are commercially available.
Embodiment 1:
1, commercially available Wu Shi acetobacter, lactobacillus acidophilus, Lactobacillus casei, propionibacterium freudenreichii are first activated respectively, Bacteria concentration after activation is 108Cfu/ml is later 1:1:1:1 mixing according to mass ratio, accesses particle with strain as fermentation Shape fermentation base.
2, by soybean and wheat soaked overnight, after draining away the water, 120 DEG C boiling 30~50 minutes, release hot material, with wheat Bran and sucrose are uniformly mixed, natural cooling;Wherein, soybean and wheat account for 90 mass parts, and the mass ratio of soybean and wheat is 1:1, Wheat bran accounts for 10 mass parts, sucrose mass parts 2%-5%, and total moisture content reaches 60%~70%.According to 0.5% inoculum concentration (v/w) strain for accessing the activation of last step, is uniformly mixed, 35~37 DEG C anaerobic fermentation 24~48 hours.When organic acid contains Amount is 35~50g/100g, terminates fermentation, dry, obtains graininess acid fermentation object, includes the above microbial cell and organic acid, Such as formic acid, lactic acid, acetic acid and propionic acid.
3, normal drying or vacuum drying under the conditions of being lower than 60 DEG C, maintains the grain shape of product, is dried to moisture 10% hereinafter, up to anti-mite agent.
Embodiment 2:
1, commercially available Wu Shi acetobacter, propionibacterium freudenreichii, lactobacillus acidophilus are activated respectively, the bacteria concentration after activation It is 108cfu/ml;It is 1:1:1 mixing according to mass ratio, it is spare as solid state fermentation strain.
2, corn soaking is stayed overnight, after draining away the water, sucrose and cavings is added, three's mass fraction is respectively 7:0.5: 2.5;The boiling 50 minutes under the conditions of 120 DEG C, when this total moisture content reach 60%~70%;28-30 DEG C is naturally cooled to, so The strain after activating in last step is accessed according to 0.2% inoculum concentration (v/w) afterwards, is uniformly mixed;35~37 DEG C it is micro- it is aerobic- Anaerobism is alternately fermented 24~48 hours, and measuring organic acid content is 35~50g/100 grams, dry, obtains graininess fermentation material.
3, vacuum will be above-mentioned dry containing the graininess fermentation material of microbial cell and organic acid under the conditions of being lower than 60 DEG C, dries Do to moisture is 8% hereinafter, up to anti-mite agent.
Embodiment 3:
1, commercially available Wu Shi acetobacter, lactobacillus acidophilus and Lactobacillus casei are activated respectively, the bacteria concentration after activation It is 108cfu/ml;It is mixed according to mass ratio 1:1:1, it is spare as fermentation strain.
2, by wheat and sorghum soaked overnight, after draining away the water, sucrose and cavings is added, four mass fractions are respectively 4: 4:1:1;The thermophilic digestion 30 minutes under the conditions of 120 DEG C, total moisture content reaches 60%~70%;28-30 DEG C is naturally cooled to, Then the strain after activating in last step is accessed according to 0.1% inoculum concentration (v/w);In 35~37 DEG C of anaerobic fermentations 24~48 Hour, organic acid content reaches 50g/100 grams, terminates fermentation;It is dry, obtain graininess fermentation material.
3, in lower than 60 DEG C vacuum that above-mentioned organic acid fermentation object is dry, and being dried to moisture is 8% hereinafter, up to anti-mite Agent.
Application experiment example 1:
Middle Y-shaped smell sensing apparatus experimental provision referring to Fig.1.Graininess anti-mite agent in embodiment 1 is weighed, being made into concentration is 0.1%, 0.3%, 0.5%, 0.8%, 1% prepare liquid soaks filter paper as experimental group medication, is placed in 1 bottom of taste source (bottle), Acarid feed (food) is placed above filter paper, as experimental group.The pure water-soaked filter paper in other side taste source (bottle) 2, puts Measure acarid feed as a control group.Air-flow (on the right side of Fig. 1) is filtered by active carbon, after distilled water humidification, into equipped with to be measured molten The taste source bottle of liquid.Gas flow rate meter is connected before being passed through taste source bottle guarantees that air-flow velocity flow in both ends is consistent.Gas passes through taste source It takes the volatile gas components of test agent after bottle out of, and converges in the middle part of Y-piece.Chicken acarid is left by Y-piece is only released to End (on the left of Fig. 1).It makes a choice in 3min, and is stopped in experimental group side and be denoted as 1 more than 1min, stopped in control group side It is denoted as 0 more than 1min, what is do not made a choice disregards, and it is every to survey the tested acarid of taking-up later, in alcohol wipe Y-piece Wall dries, and 6 groups of this experimental setup, 5 groups of experimental groups (it is that be impregnated with concentration be 0.1%, 0.3%, 0.5%, 0.8% respectively, The filter paper of 0.1% prepare liquid), 1 group of negative control, every 30 are one group.It with distillation water-soaked filter paper, puts preliminary experiment two sides Equivalent acarid feed adjusts position to selection percentage without significant difference.It should ensure that Y-shaped smell sensing apparatus two sides answer light equal during experiment It is even, and period every survey 5, taste source bottle position is exchanged once, to reject the interference in direction.Scattering ratio=(the mite of 1- experimental group Borer population/control group acarid number) × 100%.Experimental result such as table 1:
Table 1: chicken acarid tends to situation
Experimental group 1 Experimental group 2 Experimental group 3 Experimental group 4 Experimental group 5
1 (only) 14 12 5 1 0
0 (only) 16 18 25 29 30
Scattering ratio 10% 40% 80% 97% 100%
Experimental result: with the increase of the concentration of anti-mite agent, the case where acarid is intended to control sides, is more and more, it was demonstrated that should Anti-mite agent can play apparent expulsion effect to acarid, and concentration is trusted.
Application experiment example 2:
Drug combination preparation to be measured: anti-mite agent in embodiment 2 is mixed with water, and being configured to concentration is 0.1%, 0.5%, 0.8%, 1% medical fluid to be measured.
It chooses 3 acarids and infects serious chicken house.It therefrom chooses 40 diseased chickens and is equally divided into four groups, point circle raising is natural Isolation.
First the old padding of henhouse is cleaned out before medication, new padding is changed and is started medication after 2 days.Without using any medicament It is negative control group.Chicken whole body is sprayed with miniaturised nebuliser, feather is pushed aside gradually, medical fluid is made to be sprayed onto feather deep And skin, then both feet are impregnated in medical fluid.And spray henhouse.Experimental period 30 days or so, observation daily, record test situation, 1st, 3,7,16,20,25,30 day inspection record infection rate and every group of lesion quantity.Experimental result such as table 2:
Table 2: mite fall ill chicken medication effect record
Experimental result: with the increase of drug concentration, lesion number is gradually decreased, and infection rate gradually decreases, it was demonstrated that this anti-mite Agent can be obviously improved chicken acarid disease.
Application experiment example 3:
Drug combination preparation to be measured: graininess fermentation material in embodiment 3 is mixed with water, and being configured to concentration is 0.1%, 0.5%, 0.8%, 1% medical fluid to be measured.
It chooses at least three acarid and infects serious black soldier flies farm.Each farm randomly selects 4 cultivation units (as unit of basin) is equally divided into four groups, is isolated naturally.
4 groups use drug to be measured (experimental group 1~4), one group use insect Acaricides list carbonamidine (control group), one group Without using any medicament (blank group).Sprinkling in two days medical fluid 1 time, totally 3 times.Before medication the 7th day after 1 day and medication, 5cm is used Diameter cylinder is sampled in cultivation unit with five point sampling.After being impregnated with 20% salt water, acarid number is recorded.
Table 3: mite evil black soldier flies medication effect record
Experimental result: with the increase of anti-mite agent concentration, acaricidal rate is significantly improved, it was demonstrated that the agent of this anti-mite can be obviously improved black Stratiomyiid acarid disease.

Claims (10)

1. a kind of graininess anti-mite agent, which is characterized in that the graininess anti-mite agent obtains with the following method: by microbial bacteria Kind activates, and bacteria concentration is 10 after actication of culture8~1011Cfu/ml obtains acid particles shape fermentation material, so by solid state fermentation Low temperature drying is afterwards to get the anti-mite agent;Wherein, the microbial strains are that can produce formic acid bacterium, bacterium acidi propionici, acetic acid bacteria, lactic acid One of bacterium or other acid-producing bacterias are a variety of;Production formic acid bacterium is essential bacterial strain;Other acid-producing bacterias are the bacterium for producing butyric acid or valeric acid Kind;The total acidity of graininess fermentation material is 20~50g/100g (in terms of lactic acid), or higher.
2. graininess anti-mite agent according to claim 1, which is characterized in that the fermentation process of solid state fermentation: by trophism Granular base-material soaked overnight, then be added auxiliary material boiling, 120 DEG C sterilize 30 minutes, after curing, water content be 60%~ 75%, it is cooling;By the strain access after activation, ferment 24~48 hours under 30~40 DEG C, aerobic or anaerobic condition, fermentation knot Beam, it is dry.
3. graininess anti-mite agent according to claim 1, which is characterized in that produce the Wu Shi acetobacter etc. of formic acid bacterium;Propionic acid Bacterium is to produce one of sour Propionibacterium, propionibacterium freudenreichii, Xie Shi Propionibacterium, Te Shi Propionibacterium or Beijing Propionibacterium Or it is a variety of;Lactic acid bacteria is one of lactobacillus acidophilus, Lactobacillus casei, Lactococcus lactis or bifidobacterium lactis or a variety of;It produces The clostridium butyricum etc. of butyric acid, but it is not limited to the above bacterial strain;Preferentially, it selects safe for human and animal and environment, it might even be possible to directly eat Microbial strains.
4. graininess anti-mite agent according to claim 2, which is characterized in that the particulate base of solid state fermentation is soybean, beans One of the dregs of rice, paddy, rice, corn, Gao Liang, wheat or millet etc. are a variety of, and auxiliary material is wheat bran and/or cavings.
5. graininess anti-mite agent according to claim 4, which is characterized in that wherein, according to the mass fraction, graininess trophism Base-material accounts for mass fraction 80%~90%;Auxiliary material accounts for mass fraction 10%~20%, and the initial pH value of solid-state fermentation material is 5.0~ 7.2。
6. graininess anti-mite agent according to claim 2, which is characterized in that strain access amount after activation is 0.1%~ 1.0% (v/w).
7. graininess anti-mite agent according to claim 2, which is characterized in that after fermentation, common lower than at 60 DEG C or Moisture 10% is dried under vacuum to hereinafter, activity to keep mentioned microorganism cell, reduces the volatilization of all organic acids.
8. the application of any graininess anti-mite agent in claim 1 to 7, which is characterized in that by the graininess anti-mite agent It discharges in livestock and poultry, cultured insect environment or pet living environment, every square metre uses 1-10 grams;Or with grain and Feed mixing, when being used in mixed way, the anti-mite agent is 0.1%~10% (w/w) according to mass concentration.
9. graininess anti-mite agent according to claim 8 is applied to livestock and poultry, cultured insect environment or pet living environment, Be characterized in that, the organic acid in particle can slow release, after trophism particulate base absorbs the moisture content in environment, in particle Acid-producing bacteria again can continued growth, produce acid, thus strengthen and continue anti-mite effect.
10. applying according to claim 8 or claim 9, which is characterized in that the process object of anti-mite agent be colonize in livestock and poultry, pet, Harmful mite in insect body surface, breeding environment and cereal, grain and feed.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111493099A (en) * 2020-06-02 2020-08-07 上海交通大学 Compound extracted from peanut branches and leaves and used for preventing and treating tetranychus cinnabarinus and application of compound

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