KR100986306B1 - Lactobacillus plantarum BLS41 Originated from Kimchi and Use Thereof - Google Patents

Abstract

The present invention is novel kimchi-derived Lactobacillus plantarum ( Lactobacillus plantarum ) BLS41, more specifically, Lactobacillus plantarum ( Lactobacillus ) having antifungal and antibacterial activity plantarum ) BLS41 and its use.

Lactobacillus plantarum BLS41 or the growth inhibitory substance of the pathogenic bacteria produced by the lactic acid bacteria according to the present invention is useful as an ingredient of an environment-friendly microbial agent and an antibiotic-free feed.

Antifungal, growth inhibition, Lactobacillus plantarum

Description

Lactobacilli plantarum BLS41 Originated from Kimchi and Use Thereof}

The present invention is newly identified from Lactobacillus plantarum ( Lactobacillus) plantarum ) BLS41, more specifically, Kimchi-derived newly identified Lactobacillus plantarum ( Lactobacillus ) having antifungal and antibacterial activity plantarum ) BLS41 and its use.

Many differentiation types in the fusarium sp., Filamentous fungi, are parasitic on various host crops (vegetables, flowers, field crops, etc.), causing catheterization or root rot. Also accompanied by.

Fuzium's transmission forms thick-film spores, which are durable survival organs, and are contaminated with other crops or fields through crops, management devices, and soil. In the case of airborne terrestrial pests, by spraying chemicals that inhibit pathogens on the surface of the host crop, the pathogens can be killed or prevented by controlling the infection. However, after infection, the infection site is rooted, and since all infectious agents are present in the soil, it is difficult to stop the root infection because it is difficult to reach the deep soil area even if the disinfectant is applied to the toffee.

 In order to prevent the damage of Fujingum, there are various methods of cropping, rotation, and physical control. As a biological control method, strains that have high antagonism against Fujurium are selected from among the microorganisms that have high affinity with the roots of crops. After seeding, seed or young seedlings are settled in the roots of the host crop, or the same species of microorganisms, such as Fuzium, which have no pathogenicity, are inoculated into the crops or roots of the host crop as a vaccine. It is to suppress the infection of Fujurium by creating a resistant material.

The development of these conditions in ornamental plants, such as phalaenopsis, is of low coronary value, so these pathologies in the ornamental plant cultivation industry are fatal. Once a disease occurs in a plant, if it is poorly managed, it leaves a mark on the leaves, which reduces the coronary value until it becomes a deciduous plant. have.

In order to prevent the development of plant diseases, pesticides should be sprayed in a wide space, unlike the method of administering drugs such as injections or doses applied to animals, so if the toxicity is strong, it will adversely affect not only the sprayer but also people and plants and animals exposed to the spreading environment. Can cause. In order to prevent this, it is required to develop an eco-friendly biological agent that is harmless to animals and plants and has an effect of preventing plant diseases.

Lactic acid bacteria can grow rapidly at 20 ~ 40 ℃ regardless of anaerobic or aerobic conditions, and produce lactic acid to increase acidity and produce hydrogen peroxide to inhibit the growth of pathogenic microorganisms. It is known to prevent. It also improves the self-defense ability of plants by producing various bioactive substances, antibacterial substances and anticancer substances, and in the case of livestock, it shows the effect of stabilizing intestinal microorganisms, increasing feed efficiency, and increasing disease resistance. However, in order to industrially use lactic acid bacteria correctly, it is generally necessary to go through a procedure to clearly understand and evaluate from a scientific standpoint what the use is and what characteristics are suitable for use, rather than judging by a list of positive effects. Only with such a procedure can you ensure the uniformity of the product and the reproducibility of the effects.

Accordingly, the present inventors have made diligent efforts to separate lactic acid bacteria that can be used to treat and prevent ornamental plant diseases against 150 kinds of lactic acid bacteria derived from kimchi. As a result, Lactobacillus plantarum is a lactic acid bacterium. BLS41 was selected, and the present invention was completed by confirming that the strain and the culture medium derived from the strain inhibited the growth of phytopathogenic fungi including Fuzarium and pathogenic bacteria including Salmonella and Samonella .

An object of the present invention to provide a kimchi-derived Lactobacillus plantarum having antifungal and antipathogenic fungal activity.

Another object of the present invention is to provide an antifungal agent comprising Lactobacillus plantarum or its culture.

Still another object of the present invention is to provide a composition for inhibiting infection of pathogenic bacteria comprising Lactobacillus plantarum or its culture.

Still another object of the present invention is to provide a food having an infection suppression effect of a pathogenic bacterium comprising Lactobacillus plantarum or its culture.

Still another object of the present invention is to provide a feed additive having an inhibitory effect on infection of pathogenic bacteria including Lactobacillus plantarum or its culture.

In order to achieve the above object, the present invention has the activity of anti-fungal and anti-pathogenic bacteria Kimchi-derived Lactobacillus plantarum ( Lactobacillus plantarum ) provides BLS41 KCTC 11056BP.

The present invention also provides an antifungal agent comprising Lactobacillus plantarum BLS41 KCTC 11056BP or a culture thereof.

The present invention also provides a composition for inhibiting infection of pathogenic bacteria comprising Lactobacillus plantarum BLS41 KCTC11056BP or a culture thereof.

The present invention also provides a food having an infection suppression effect of a pathogenic bacterium comprising Lactobacillus plantarum BLS41 KCTC11056BP or a culture thereof.

The present invention also provides a feed additive having an infection inhibitory effect of a pathogenic bacterium comprising Lactobacillus plantarum BLS41 KCTC11056BP or a culture thereof.

Novel Lactobacillus having the anti-fungal and anti-pathogenic activity of the bacteria isolated from kimchi according to the invention Planta room (Lactobacillus plantarum ) Since BLS41 KCTC11056BP is isolated from kimchi, its stability is guaranteed even when ingesting live bacteria, and it is effective in inhibiting the growth of pathogens of plants and animals by microorganisms. useful.

In one aspect, the present invention relates to kimchi-derived Lactobacillus plantarum BLS41 KCTC 11056BP having the activity of antifungal and antipathogenic bacteria.

Lactobacillus plantarum It is a gram-positive bacterium belonging to the genus Lactobacillus , which is commonly found in kimchi or sauerkraut, pickles, pickled olives, and lactic acid bacteria found in plant fermented foods and some cheeses. The Lactobacillus plantarum BLS41 of the present invention is newly identified New microorganisms.

Since the microorganism of the present invention is known as a bacterium which is usually identified as a lactic acid bacterium isolated from kimchi, it is obvious to those skilled in the art that it is harmless to the human body even when ingested as live bacteria.

The microorganism of the present invention has antimicrobial, antifungal and growth inhibitory activity against pathogenic bacteria and fungi. The antibacterial and antifungal activity is characterized by stable under high temperature, and inhibiting the growth of the Phalaenopsis causative agents of ewiniah causing galbanbyeong and yeonbubyeong (Erwinia) and Pseudomonas (Pseudomonas), and further causative agents of Puja causing conduit disease and root rot Fusarium also exhibits growth inhibition. In particular, the action was found to be better than the therapeutic effect preventive effect.

In another aspect, the present invention relates to an antifungal agent having a growth inhibitory or killing effect of a pathogenic bacterium or fungus comprising Lactobacillus plantarum BLS41 KCTC 11056BP or a culture thereof.

The antifungal agent of the present invention exhibits antifungal action against the genus Fusarium , which causes catheter diseases and root rot, Erwinia genus and Pseudomonas genus, which cause branching and soft disease in phalaenopsis.

As used herein, the term “antifungal agent” refers to an agent having an effect that helps plant cultivation when used in soil or plants as a microbial agent due to the effect of the activity of certain useful microorganisms contained in the agent. The antifungal agent of the present invention contains live bacteria, and may optionally further include a carrier which adsorbs the cells, an organonutrient for activating the cells, and other auxiliary agents. Efficacy of antifungal preparations is to promote organic decomposition, inhibit rotation, improve soil physicochemical properties, improve soil microbiality, improve crop productivity, improve crop quality, clean up pollutants, and prevent pests. Microbial formulations using Planta room BLS41 is intended to achieve the effect of plant growth and pest prevention.

Antifungal formulations containing the Lactobacillus plantarum BLS41 culture of the present invention are liquid suspensions, stabilized liquid suspensions, emulsified concentrated formulations, capsules, water or hydrous powders, aqueous flowables, dry flowables ); It can be prepared in the form of hydrate granules and hydrate dispersing granules and the like, the state of the microbial agent is well known by those skilled in the art. In addition, the strains or cultures of the present invention can be used with fertilizers, nutrient soils or side sprays, and other formulation states are apparent to those of ordinary skill in the art.

Antifungal preparations comprising Lactobacillus plantarum BLS41 of the present invention can be used to obtain an antimicrobial effect when cultivating rice, vegetables, fruit trees, flowers, grass, east and west eggs, more preferably antibacterial when cultivating East and West eggs Can be used to get the effect.

The antifungal formulations of the invention can be treated on soil, seeds, seedlings or plants, and can be applied by manual means or by mechanical means using injectors or tilling systems. Since it is difficult to predict when a disease may occur during cultivation, in order to use a microbial preparation containing the strain of the present invention to maintain a desired cultivation state, it may be repeatedly sprayed several times depending on the cultivation time and cultivation environment. desirable. Typically, the application of lactic acid bacteria for plant growth and pest prevention is 5-20 days, and more preferably 7-15 days. If possible, it is very important to spray before exposure to pests.

Methods used in plants to obtain the effect of antifungal agents can be readily performed by one of ordinary skill in the art.

The antifungal and antipathogenic fungal activity of the present invention has the same efficacy in Lactobacillus plantarum BLS41 and cultures derived from the strain.

As used herein, the term 'culture' may be a culture stock solution including the cells, and may also be a culture supernatant or a dilution of the culture.

The composition of the culture may further include not only components necessary for normal lactic acid bacteria culture, but also components that synergistically act on the growth of lactic acid bacteria, and thus the composition may be easily selected by those skilled in the art. Can be.

The strain and culture may be prepared as an antimicrobial composition having an antifungal agent for agriculture, a composition for inhibiting infection of pathogenic bacteria, a growth inhibitory effect of pathogenic bacteria and fungi including food and animal feed additives, but is not limited thereto.

In addition, the antifungal agent of the present invention, the composition for inhibiting infection of pathogenic bacteria, food and animal feed additives may include only cultures from which microorganisms have been removed, and the cells may be mixed or manufactured to contain only cells.

In order to prepare the antimicrobial composition of the present invention, the Lactobacillus plantarum BLS41 of the present invention can be cultured in large quantities through conventional liquid or solid phase culture techniques. The antifungal agent and composition of the present invention may be composed of the feeder cells or spores of the strain, or a mixture of feeder cells and spores, and the cells are harvested under reduced pressure freeze-drying (lyophilized), lyophilization, and hydration for preparation. can do.

Lactobacillus Planta room BLS41 of the present invention exhibit antibacterial activity against pathogenic bacteria in the intestinal Salmonella (Salmonella) and in Bacillus (Bacillus) to cause food poisoning. Also, in addition to antibacterial activity against plant pathogenic fungi.

Therefore, in another aspect, the present invention relates to a composition for inhibiting infection of pathogenic bacteria comprising Lactobacillus plantarum BLS41 KCTC 11056BP or a culture thereof.

The microorganism may be administered orally, parenterally, or physically in contact with the skin or mucous membranes together with a pharmaceutically acceptable carrier substrate or nutrient, or directly to the intestine via a tube or catheter.

The composition may be prepared in tablets, capsules, implants, suppositories, powders, solutions, gels, liquid strain suspensions, and the like.

The dosage of the composition can be determined by one of ordinary skill in the art, and can be determined by a person skilled in the art, such as formulating method, mode of administration, age, weight, sex, morbidity, food, time of administration, route of administration, rate of excretion and response of the recipient. Various factors may also be prescribed.

In yet another aspect, the present invention relates to a food (or health functional food) having an infection inhibitory effect of a pathogenic bacterium comprising Lactobacillus plantarum BLS41 KCTC 11056BP or a culture thereof.

Lactobacillus, such as Lactobacillus, acts as a beneficial bacterium for the human body by lactic acid fermentation to prevent food spoilage, secrete antibacterial substances to suppress food poisoning bacteria. Food produced using lactic acid bacteria fermentation include cheese, buttermilk, yogurt, whey (whey), etc. Recently, functional yogurt by lactic acid bacteria fermentation is also widely marketed. In addition, lactic acid bacteria fermented foods are manufactured as tablets, granules, and the like as commercial preparations and are commercially available as food compositions and pharmaceuticals.

Therefore, the food is refrigerated, frozen or long-term dairy products, such as milk, milk powder, yogurt, kefir, ice cream, milkshake, cheese, cream, curd, fermented milk, and milk is added As well as fermented foods may include soy milk, fermented grain products, patient diets and infant diets, etc. To culture the Lactobacillus plantarum BLS41 of the present invention to produce or add to food, starch such as milk or cereals Substrates included are preferred for strain culture.

In the present invention, the food may include one or more health functional compositions or nutritional components composed of fat, protein, carbohydrates, dietary fiber, minerals and vitamins, etc., together with the microorganism of the present invention. In particular, the dietary fiber is known as a prebiotic substrate that promotes the cleavage of lactic acid bacteria, so it may enter the body and play an important role in the formation and maintenance of colonies in the intestine. It may be included in the composition so that it can be administered later or simultaneously.

In still another aspect, the present invention relates to a feed additive having an infection inhibitory effect of a pathogenic bacterium comprising Lactobacillus plantarum BLS41 KCTC 11056BP or a culture thereof.

Feed composition of the present invention is to replace the existing antibiotics and inhibit harmful microorganisms to improve the health of the animal body, improve the weight gain and meat quality of the livestock, and has the effect of increasing the milk yield and immunity. Feed composition of the present invention can be prepared in the form of fermented feed, compound feed, pellet form and silage and the like. The effective amount of Lactobacillus plantarum BLS41 added to the feed is at least 10 ⁶ / g, preferably at least 10 ⁸ / g. Fermented feed can be prepared by fermenting an organic material by adding various microorganisms or enzymes, compounded feed can be prepared by mixing a variety of general feed and microorganism of the present invention. Pellet-type feed may be prepared by applying heat and pressure to the blended feed and the like in a pellet machine, silage can be prepared by fermenting the green feed to the microorganism according to the present invention. Wet fermented feed collects and transports organic materials such as food waste, mixes excipients for sterilization process and moisture control at a certain ratio, and then ferments at a temperature suitable for fermentation for 24 hours or more to control about 70% water content. It can be prepared by. Fermented dry irrigated feed may be prepared by adjusting the wet fermented feed to contain about 30% to 40% of water by further drying.

The antifungal agent of the present invention, the composition for inhibiting infection of pathogenic bacteria, food and feed additives may additionally include a probiotic strain in addition to a pharmaceutically necessary composition or a composition suitable for food.

The amount of Lactobacillus plantarum BLS41 included in the pharmaceutical carrier or food may be about 10 ⁵ cfu / g to about 10 ¹¹ cfu / g, preferably 10 ⁶ cfu / g to about 10 ¹⁰ cfu / g, Most preferably from 10 ⁷ cfu / g to about 10 ⁹ cfu / g. In the case of administering the strain, it is preferable to administer in a viable state, it may be killed before ingestion, or may be administered in an attenuation state. In addition, in the case of using the culture, the sterilization process through heat treatment may be additionally performed. The amount of strain and the daily intake required to have the minimum efficacy may vary depending on the body or health status of the ingestor, but in general, 10 ⁶ to 10 ¹² cfu / day is preferred, most preferably 10 ⁷ to 10 ¹⁰ cfu / It is day. The culture supernatant can be used to determine the degree of antimicrobial efficacy using techniques commonly used in the art, and thus the dosage can be determined.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it will be apparent to those skilled in the art that the scope of the present invention is not limited to these examples.

Example  One : Lactobacillus Planta Room BLS41 Strain selection

Pathogens causing phalaenopsis in plant diseases are cultivated from Korea Agricultural Microorganisms Preservation Center, incubated and spread evenly with smears on LB plates, and the culture supernatants of 150 kinds of lactic acid bacteria isolated from kimchi are dipped onto the plates. Incubated. After incubation, lactic acid bacteria having antimicrobial activity against branched and soft disease bacteria were selected for 1 week based on whether or not clear zones were generated on LB plates.

16S rRNA coding DNA site based on 16S rDNA nucleotide sequence of lactic acid bacteria registered on GenBank (GenBank, www.ncbi.nlm.nih.gov) to perform 16S rDNA sequencing for identification of selected lactic acid bacteria Primers capable of amplifying (SEQ ID NO: 1 and SEQ ID NO: 2) were prepared.

SEQ ID NO: 5'-cag tga cga ctc tgg tat tga-3 '

SEQ ID NO: 5'-cct aat cat ctg tcc cac ctt-3 '

PCR was performed using a pair of primers as a template with lysates of 10 ⁹ lactic acid bacteria cells to obtain a DNA fragment of 1439 bp, which was cloned into a pGEM-Teasy vector (Promega) and analyzed for nucleotide sequence ( SEQ ID NO: 3). As a result, the selected lactic acid bacteria were found to have more than 99% homology with Lactobacillus plantarum (Accession No DQ239696.1), Lactobacillus plantarum (Accession No AL935261.1).

As a result, the Lactobacillus Lactobacillus plantarum BLS41 having an antimicrobial activity against phalaenopsis pathogenic bacteria was named, and deposited on KCTC as of December 26, 2006 (KCTC 11056BP).

Example  2 : Lactobacillus Planta Room BLS41 Antimicrobial activity of

In order to confirm the thermostability of the antimicrobial activity of Lactobacillus plantarum BLS41 KCTC11056BP, the culture supernatant was heat-treated at different temperatures to observe the change of antimicrobial activity. MRS medium used as a result controls the phytopathogenic gyunin Pseudomonas kateul LEA (Pseudomonas cattleyae) smear a 50 ℃ on solid medium, 80 ℃, 15 minutes at 100 ℃, processing a Lactobacillus Planta room BLS41 culture supernatant was heat-treated for 30 minutes with a ( 1% casein hydrolysate, 1.5% yeast extract, 2% destrose, 0.2% ammonium citrate, 0.5% sodium acetate, 0.01% magnesium sulfate, 0.05% manganese sulfate and 0.2% dipotassium phosphate , Lactobacillus plantarum BLS41 cultures were dotting the antimicrobial activity was observed. When the experiments under the same conditions were performed on Erwinia chrysanthemii , it was confirmed that the growth of pathogenic bacteria was inhibited by Lactobacillus plantarum BLS41 culture supernatant (FIG. 1). On the basis of the above results, it was confirmed that the Lactobacillus plantarum BLS41 culture solution had a heat-stable secretory component that inhibited the growth of two pathogenic bacteria of the phalaenopsis.

Example  3: Lactobacillus Planta Room BLS41 of Plant disease  Inhibitory effect of symptoms

Lactobacillus plantarum BLS41 culture stock solution, culture supernatant from which cells were removed after culture, and heat-treated culture supernatant were sprayed and sealed on the phalaenopsis showing disease, and the experimental control group without the culture solution was dried and leaves. In the group treated with BLS41 culture supernatant, culture supernatant, and heat-treated culture supernatant, Lactobacillus plantarum BLS41 cultured leaves became deciduous, but no more deciduous leaves were developed and the pathology was alleviated (FIG. 2).

Example  4 : Lactobacillus Planta Room BLS41 of Fuzium ( Fusarium oxysporum )on  Antifungal activity against

Phytopathogenic fuzarium (KACC41090) was distributed from the Korea Agricultural Microbial Resources Center and the antifungal activity of Lactobacillus plantarum BLS41 was observed. Dispense 50 μl of PDB medium (20% potato infusion frum, 2% bactodextrose) containing 2 × 10 ³ fujium in a 96-well plate and dilute 1/2 fold of the Lactobacillus plantarum BLS41 culture sequentially. After mixing with fungi and incubating for 16 hours at 37 ℃, the culture solution was plated in PDB solid medium and further cultured at 30 ℃. As a result, Fusarium colonies occurred in the solid medium to which the MRS lactic acid bacteria culture medium was added, but Lactobacillus plantarum BLS41 was found to be antifungal because no Fusarium colonies were formed in the solid medium to which Lactobacillus plantarum BLS41 was added. It was shown to have activity (FIGS. 3 and 4).

Example  5: Lactobacillus Planta Room BLS41 Antimicrobial activity against animal pathogenic bacteria

Carried out in the same manner as the method of Example 3 Salmonella typhimurium Lactobacillus Bacillus Planta room BLS41 culture supernatant bunch Titanium (Salmonella typhimurium, Korea Research Institute of Bioscience and Biotechnology Biological Resource Center, KCTC 1925) and Bacillus cereus (Bacillus cereus , Korea Institute of Bioscience and Biotechnology, and KCTC 3624), were applied to the smeared solid media to observe the effect of growth inhibition on the pathogenic bacteria. As a result, it was confirmed that the growth inhibitory effect (Fig. 5).

The specific parts of the present invention have been described in detail above, and it is apparent to those skilled in the art that such specific descriptions are merely preferred embodiments, and thus the scope of the present invention is not limited thereto. something to do. Thus, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

1 is a Lactobacillus plantarum Changes in the antimicrobial activity of BLS41 culture supernatant according to temperature and heat treatment time.

Figure 2 is Lactobacillus plantarum BLS41 culture stock solution, supernatant culture solution and heat-treated culture solution were shown to be antimicrobial effect on the plant disease obtained after spraying the phalaenopsis egg is observed.

Figure 3 is Lactobacillus plantarum ( Lactobacillus plantarum ) shows antimicrobial activity against Fusarium of BLS41 and its culture.

Figure 4 is Lactobacillus plantarum ( Lactobacillus) plantarum ) shows the antimicrobial activity of BLS41 against Fusarium .

5 is other pathogenic bacteria besides phalaenopsis pathogenic bacteria ( Salmonella typhimurium , Bacillus cereus ) Lactobacillus plantarum It shows the antibacterial effect of BLS41.

<110> BioLeaders Corp.          Korea Research Institute of Bioscience and Biotechnology          University-Industry Collaboration Foundation Chungnam National University <120> Lactobacillus pantarum BLS41 Originated from Kimchi and Use          Thereof <160> 3 <170> KopatentIn 1.71 <210> 1 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Forward Primer <400> 1 cagtgacgac tctggtattg a 21 <210> 2 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> Reverse Primer <400> 2 cctaatcatc tgtcccacct t 21 <210> 3 <211> 1439 <212> DNA <213> Lactobacillus 16S rDNA <400> 3 ggtattgatt ggtgcttgca tcatgattta catttgagtg agtggcgaac tggtgagtaa 60 cacgtgggaa acctgcccag aagcggggga taacacctgg aaacagatgc taataccgca 120 taacaacttg gaccgcatgg tccgagcttg aaagatggct tcggctatca cttttggatg 180 gtcccgcggc gtattagcta gatggtgggg taacggctca ccatggcaat gatacgtagc 240 cgacctgaga gggtaatcgg ccacattggg actgagacac ggcccaaact cctacgggag 300 gcagcagtag ggaatcttcc acaatggacg aaagtctgat ggagcaacgc cgcgtgagtg 360 aagaagggtt tcggctcgta aaactctgtt gttaaagaag aacatatctg agagtaactg 420 ttcaggtatt gacggtattt aaccagaaag ccacggctaa ctacgtgcca gcagccgcgg 480 taatacgtag gtggcaagcg ttgtccggat ttattgggcg taaagcgagc gcaggcggtt 540 ttttaagtct gatgtgaaag ccttcggctc aaccgaagaa gtgcatcgga aactgggaaa 600 cttgagtgca gaagaggaca gtggaactcc atgtgtagcg gtgaaatgcg tagatatatg 660 gaagaacacc agtggcgaag gcggctgtct ggtctgtaac tgacgctgag gctcgaaagt 720 atgggtagca aacaggatta gataccctgg tagtccatac cgtaaacgat gaatgctaag 780 tgttggaggg tttccgccct tcagtgctgc agctaacgca ttaagcattc cgcctgggga 840 gtacggccgc aaggctgaaa ctcaaaggaa ttgacggggg cccgcacaag cggtggagca 900 tgtggtttaa ttcgaagcta cgcgaagaac cttaccaggt cttgacatac tatgcaaatc 960 taagagatta gacgttccct tcggggacat ggatacaggt ggtgcatggt tgtcgtcagc 1020 tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc aacccttatt atcagttgcc 1080 agcattaagt tgggcactct ggtgagactg ccggtgacaa accggaggaa ggtggggatg 1140 acgtcaaatc atcatgcccc ttatgacctg ggctacacac gtgctacaat ggatggtaca 1200 acgagttgcg aactcgcgag agtaagctaa tctcttaaag ccattctcag ttcggattgt 1260 aggctgcaac tcgcctacat gaagtcggaa tcgctagtaa tcgcggatca gcatgccgcg 1320 gtgaatacgt tcccgggcct tgtacacacc gcccgtcaca ccatgagagt ttgtaacacc 1380 caaagtcggt ggggtaacct tttaggaacc agccgcctaa ggtgggacag atgattagg 1439  

Claims (9)

Ewiniah (Erwinia), A Puja Solarium (Fusarium) genus Pseudomonas (Pseudomonas) in Salmonella (Salmonella) in and Bacillus-derived Lactobacillus Planta room kimchi with antibacterial activity against in (Bacillus) (Lactobacillus plantarum) BLS41 KCTC 11056BP. Claim in claim 1. Lactobacillus Planta room (Lactobacillus plantarum) BLS41 KCTC 11056BP or cultured in ewiniah (Erwinia) containing water as an active ingredient, Puja Solarium (Fusarium) genus Pseudomonas (Pseudomonas) in Salmonella (Salmonella) and Antimicrobial composition against the genus Bacillus . Paragraph 1 Lactobacillus Planta Room (Lactobacillus plantarum) BLS41 KCTC 11056BP or includes culture as an active ingredient, ewiniah that cause plant diseases (Erwinia) genus in Fujairah Leeum (Fusarium) in and Pseudomonas (Pseudomonas) in the strain An antifungal agent having antimicrobial activity against. delete delete delete delete delete delete

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