CN111493099A - Compound extracted from peanut branches and leaves and used for preventing and treating tetranychus cinnabarinus and application of compound - Google Patents
Compound extracted from peanut branches and leaves and used for preventing and treating tetranychus cinnabarinus and application of compound Download PDFInfo
- Publication number
- CN111493099A CN111493099A CN202010489359.5A CN202010489359A CN111493099A CN 111493099 A CN111493099 A CN 111493099A CN 202010489359 A CN202010489359 A CN 202010489359A CN 111493099 A CN111493099 A CN 111493099A
- Authority
- CN
- China
- Prior art keywords
- leaves
- tetranychus cinnabarinus
- compound
- peanut
- preventing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
- A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
- A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
- C07C51/42—Separation; Purification; Stabilisation; Use of additives
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07J—STEROIDS
- C07J9/00—Normal steroids containing carbon, hydrogen, halogen or oxygen substituted in position 17 beta by a chain of more than two carbon atoms, e.g. cholane, cholestane, coprostane
Abstract
The invention discloses a tetranychus cinnabarinus prevention and treatment compound extracted from peanut branches and leaves, wherein the tetranychus cinnabarinus prevention and treatment compound comprises palmitic acid, isovanillic acid, β -sitosterol and stigmastane-3, 6-diol.
Description
Technical Field
The invention relates to the technical field of botanical agricultural acaricides as pesticides, in particular to a tetranychus cinnabarinus preventing compound extracted from peanut branches and leaves and application thereof.
Background
Tetranychus cinnabarinus (Tetranychus cinnabarinus): red spider, belonging to the order Acarina, Tetranychidae; the growth and development characteristics of the plant are small volume, rapid propagation, multiple generations (one generation in summer is 10 days), strong adaptability and easy generation of drug resistance; tetranychus cinnabarinus host plants of up to 146 species (cotton, soybean, vegetables, fruit trees, flowers and the like) in 45 families are harmful to leaves under the conditions of high temperature and low humidity, so that the leaves are green and yellow, and are withered or even fall off when the leaves are serious, and the yield and the quality are reduced.
At present, chemical pesticides containing chlorine and phosphorus, such as amitraz, trichlorfon, vozapyr and the like, are mostly used for preventing and treating tetranychus cinnabarinus. The chemically synthesized pesticide has a single action mode, and can cause mites to generate drug resistance after being used for a large amount for a long time, so that the control difficulty is increased; the chemosynthetic acaricide is not easy to degrade in the environment, has strong toxicity, is harmful to agricultural beneficial insects, and can pollute water resources due to residue in soil, thereby being harmful to the ecological environment. With the implementation of the relevant environmental protection policies of the country, chemical pesticides with strong toxicity are eliminated, other chemical synthetic pesticides are gradually eliminated from the market, and the research and development of low-residue, low-toxicity or nontoxic pesticides are urgently needed to meet the dual effects of preventing and treating agricultural mites and protecting the environment. Finding bioactive substances from biological resources, especially plant resources, and developing new pesticides have become an important approach for pesticide development.
At present, extracts of various plants have control activity on tetranychus cinnabarinus, extracts of black nightshade root and sun euphorbia root, copperleaf herb, houttuynia cordata ethanol extract and inula flower extract have high contact killing activity on tetranychus cinnabarinus, and the research of separating and purifying active compounds from branches and leaves of peanut (Arachis Hypogaea L.) for control of tetranychus cinnabarinus is not seen in the current research.
Therefore, the technicians in the field are dedicated to develop a method for preparing acaricide by separating and extracting active compounds from peanut crude extract and adding an auxiliary agent to be applied to control tetranychus cinnabarinus, so that tetranychus cinnabarinus can be effectively controlled, the harm degree is reduced, the economic loss in agricultural production is reduced, and the method has the characteristics of no public hazard, no pollution and no residue and avoids the harm of chemical synthetic pesticides.
Disclosure of Invention
In view of the defects in the prior art, the invention aims to solve the technical problem of developing a method for preparing an acaricide by separating and extracting an active compound from a peanut crude extract and adding an auxiliary agent to be applied to control tetranychus cinnabarinus, so that tetranychus cinnabarinus can be effectively controlled, the harm degree is reduced, the economic loss in agricultural production is reduced, and the acaricide has the characteristics of no public hazard, no pollution and no residue, and avoids the harm of chemical synthetic pesticides.
In order to achieve the above object, the present invention provides in a first aspect a tetranychus cinnabarinus preventing and treating compound extracted from peanut branches and leaves, wherein the tetranychus cinnabarinus preventing and treating compound comprises palmitic acid, isovanillic acid, β -sitosterol and stigmastane-3, 6-diol;
the palmitic acid has a structure represented by formula (1):
the isovanillic acid has a structure represented by formula (2):
the β -sitosterol has a structure represented by formula (3):
the stigmastane-3, 6-diol has a structure shown in a formula (4):
the invention provides a method for extracting a compound for preventing and treating tetranychus cinnabarinus from peanut branches and leaves, which comprises one or more of extraction method, thin-plate chromatography, normal-pressure column chromatography, medium-pressure column chromatography and high performance liquid chromatography; the tetranychus cinnabarinus prevention and control compound is extracted from a peanut branch and leaf crude extract, and the peanut branch and leaf crude extract is obtained by soaking and extracting plants in Arachis in leguminosae by using a solvent.
Further, the thin plate chromatography is to place the crude extract of the peanut branches and leaves on a thin plate paved with silica gel for separation.
Further, the normal pressure column chromatography is to place the crude extract of the peanut branches and leaves in a glass column with a silica gel stationary phase for separation.
Further, the medium-pressure column chromatography is to separate the substances separated by the normal-pressure column chromatography in a glass column with the stationary phase of C18.
Further, the high performance liquid chromatography is to put the substances separated by the medium pressure column chromatography into a chromatographic column for high pressure separation.
Further, the solvent is one or more of petroleum ether, ethyl acetate, n-butanol, methanol, chloroform and water.
Further, the ratio of the petroleum ether to the ethyl acetate is 1:0-0:1, the ratio of the ethyl acetate to the methanol is 1:0-0:1, the ratio of the n-butanol to the water is 2:1, the ratio of the methanol to the water is 1:0-0:1, and the ratio of the chloroform to the water is 1:0-0: 1.
The third aspect of the invention provides a use method of the tetranychus cinnabarinus preventing and treating compound extracted from peanut branches and leaves, which comprises the following steps:
(1) preparing: inoculating 30-60 heads of tetranychus cinnabarinus into the leaves;
(2) soaking, namely soaking the leaves in the tetranychus cinnabarinus preventing and controlling compound with the concentration of 0.5-2mg/m L for 5s, and taking out the leaves;
(3) cleaning: putting the taken-out leaves into clear water, and repeating for 3 times;
(4) counting: and (3) culturing the tetranychus cinnabarinus on the cleaned leaves under normal conditions, and counting the death rate after 24 hours.
The fourth aspect of the invention provides a preparation method of the acaricide based on the tetranychus cinnabarinus prevention and treatment compound extracted from peanut branches and leaves, wherein four tetranychus cinnabarinus prevention and treatment compounds are mixed with an auxiliary agent, and the auxiliary agent is one or more of tween 80, N-dimethyldecanamide, methyl oleate and methyl oleate emulsifier.
The invention has the following beneficial technical effects: the method for preparing the acaricide by separating and extracting the active compound from the crude peanut extract and adding the auxiliary agent to be applied to preventing and controlling tetranychus cinnabarinus is developed, so that tetranychus cinnabarinus is effectively prevented and controlled, the harm degree is reduced, the economic loss in agricultural production is reduced, and the acaricide has the characteristics of no public hazard, no pollution and no residue, and avoids the harm of chemosynthesis pesticides.
The conception, the specific structure and the technical effects of the present invention will be further described with reference to the accompanying drawings to fully understand the objects, the features and the effects of the present invention.
Drawings
FIG. 1 is the palmitic acid structural formula of example 1 of the present invention;
FIG. 2 is the structural formula of isovaleric acid of example 2 of the present invention;
FIG. 3 is the β -sitosterol structural formula of example 3 of the present invention;
FIG. 4 is a structural formula of stigmastane-3, 6-diol of example 4 of the present invention.
Detailed Description
The following describes several preferred embodiments of the present invention to make the technical contents thereof clearer and easier to understand. The present invention may be embodied in many different forms of embodiments and the scope of the invention is not limited to the embodiments set forth herein.
The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that variations and modifications can be made by persons skilled in the art without departing from the spirit of the invention. All falling within the scope of the present invention.
Example 1
The active compound extracted from the peanut branch and leaf crude extract is applied to a tetranychus cinnabarinus prevention and control test:
weighing 20g of peanut branch and leaf crude extract, using silica gel as a stationary phase, selecting petroleum ether-ethyl acetate (volume ratio of 1:0-0:1) and ethyl acetate-methanol (volume ratio of 1:0-0:1) as a mobile phase to obtain a plurality of crude extract solutions, using C18 as the stationary phase and selecting methanol-water (volume ratio of 1:0-0:1) or chloroform-methanol (volume ratio of 1:0-0:1) as the mobile phase to separate and purify the crude extract solutions to obtain a purified product, using a high performance liquid chromatography to determine that an active compound is palmitic acid by comparing with a standard product, using the separated and purified compound to perform a control test on tetranychus cinnabarinus, wherein the test method adopts a tetranychus maceration method in the national southern pesticide creation center for growth test, and the contact activity of the palmitic acid on the tetranychus cinnabarinus is 77.64% (1mg/m L).
Example 2
The active compound extracted from the peanut branch and leaf crude extract is applied to a tetranychus cinnabarinus prevention and control test:
weighing 20g of peanut branch and leaf crude extract, using silica gel as a stationary phase, selecting petroleum ether-ethyl acetate (volume ratio of 1:0-0:1) and ethyl acetate-methanol (volume ratio of 1:0-0:1) as a mobile phase to obtain a plurality of crude extract solutions, using C18 as the stationary phase and selecting methanol-water (volume ratio of 1:0-0:1) or chloroform-methanol (volume ratio of 1:0-0:1) as the mobile phase to separate and purify the crude extract solutions to obtain a purified product, using a high performance liquid chromatography to compare with a standard product to determine that an active compound is isovanillic acid, using the compound obtained by separation and purification to perform a prevention and control test on tetranychus cinnabarinus, wherein the test method adopts a tetranychus maceration method in national southern pesticide creation center to perform a contact killing activity of the isovanillic acid on tetranychus cinnabarinus (1mg/m L).
Example 3
The active compound extracted from the peanut branch and leaf crude extract is applied to a tetranychus cinnabarinus prevention and control test:
weighing 20g of peanut branch and leaf crude extract, using silica gel as a stationary phase, selecting petroleum ether-ethyl acetate (volume ratio of 1:0-0:1) and ethyl acetate-methanol (volume ratio of 1:0-0:1) as a mobile phase to obtain a plurality of crude extract solutions, using C18 as the stationary phase and selecting methanol-water (volume ratio of 1:0-0:1) or chloroform-methanol (volume ratio of 1:0-0:1) as the mobile phase to separate and purify the crude extract solutions to obtain a purified product, using high performance liquid chromatography to compare with a standard product to obtain an active compound of β -sitosterol, using the compound obtained by separation and purification to perform a control test on the tetranychus cinnabarinus, wherein the test method adopts a tetranychus maceration dipping method in national southern pesticide creation center to perform a touch activity of β -sitosterol to the tetranychus cinnabarinus (75.30% (1mg/m L).
Example 4
The active compound extracted from the peanut branch and leaf crude extract is applied to a tetranychus cinnabarinus prevention and control test:
weighing 20g of peanut branch and leaf crude extract, using silica gel as a stationary phase, selecting petroleum ether-ethyl acetate (volume ratio of 1:0-0:1) and ethyl acetate-methanol (volume ratio of 1:0-0:1) as a mobile phase to obtain a plurality of crude extract solutions, using C18 as the stationary phase and selecting methanol-water (volume ratio of 1:0-0:1) or chloroform-methanol (volume ratio of 1:0-0:1) as the mobile phase to separate and purify the crude extract solutions to obtain a purified product, using high performance liquid chromatography to compare with a standard product to obtain an active compound stigmastane-3, 6-diol, using the separated and purified compound to perform a control test on tetranychus cinnabarinus, wherein the test method adopts a tetranychus maceration method in national southern pesticide creation center to perform a contact killing activity of stigmane-3, 6-diol on tetranychus cinnabarinus of 74.8% (1mg/m L).
Example 5
The active compound and the auxiliary agent are mixed to form the plant source acaricide, and the acaricide is applied to the control test of tetranychus cinnabarinus:
respectively weighing methyl oleate and methyl oleate emulsifier (the mass ratio of methyl oleate to methyl oleate emulsifier is 1:0-0:1) in a beaker, adding palmitic acid (the mass ratio of palmitic acid to the solution is 1:0-0:1), stirring to fully dissolve, respectively adding N, N-dimethyldecanamide and Tween 80 (the mass ratio of the N, N-dimethyldecanamide to the solution is 1:0-0:1), stirring to fully mix uniformly to prepare the plant source acaricide, performing biological activity determination on tetranychus cinnabarinus by using the plant source acaricide through an indoor pot culture method, counting the death rate after 24 hours, wherein the control effect is 83.11% (80 mg/L).
Example 6
The active compound and the auxiliary agent are mixed to form the botanical acaricide and are applied to the tetranychus cinnabarinus control test:
respectively weighing methyl oleate and methyl oleate emulsifier (the mass ratio of methyl oleate to methyl oleate emulsifier is 1:0-0:1) in a beaker, adding isovanillic acid (the mass ratio of isovanillic acid to the solution is 1:0-0:1), stirring to fully dissolve, respectively adding N, N-dimethyldecanamide and Tween 80 (the mass ratio of N, N-dimethyldecanamide to the solution is 1:0-0:1), stirring to fully mix uniformly to prepare the plant source acaricide, performing biological activity determination on tetranychus cinnabarinus by using the plant source acaricide through an indoor pot culture method, counting the death rate after 24 hours, and achieving the control effect of 74.30% (80 mg/L).
Example 7
The active compound and the auxiliary agent are mixed to form the botanical acaricide and are applied to the tetranychus cinnabarinus control test:
respectively weighing methyl oleate and methyl oleate emulsifier (the mass ratio of methyl oleate to methyl oleate emulsifier is 1:0-0:1) in a beaker, adding β -sitosterol (the mass ratio of methyl oleate to methyl oleate emulsifier is 1:0-0:1), stirring to fully dissolve, respectively adding N, N-dimethyldecanamide and Tween 80 (the mass ratio of N, N-dimethyldecanamide to the solution is 1:0-0:1), stirring to fully mix, and preparing the plant-derived acaricide.
Example 8
The active compound and the auxiliary agent are mixed to form the botanical acaricide and are applied to the tetranychus cinnabarinus control test:
methyl oleate and methyl oleate emulsifiers (the mass ratio of methyl oleate to methyl oleate emulsifier is 1:0-0:1) are respectively weighed and placed in a beaker, stigmastane-3, 6-diol (the mass ratio of stigmastane-3, 6-diol to the solution is 1:0-0:1) is added, after the stigmast is fully dissolved, N-dimethyldecanamide and Tween 80 (the mass ratio of stigmast to the solution is 1:0-0:1) are respectively added, the mixture is fully mixed to prepare the plant source acaricide, the plant source acaricide is used for carrying out biological activity determination on tetranychus cinnabarinus by adopting an indoor pot culture method, the mortality is counted after 24 hours, and the control effect is 78.42% (80 mg/L).
The above examples simply list the method of applying the active compound separated from the crude extract of peanut branches and leaves to the control of tetranychus cinnabarinus, and list the method of preparing the plant source acaricide from the active compound. The above examples show that the present invention contains many variables, and the relationship between the variables and the control results cannot be judged according to the change of one variable, but the application of the active compound in tetranychus cinnabarinus is carried out through the coordination combination of a plurality of variables.
The foregoing description of specific embodiments of the present invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes and modifications may be made by one skilled in the art within the scope of the appended claims without departing from the spirit of the invention. The technical solutions available to a person skilled in the art through logical analysis, reasoning or limited experiments based on the prior art according to the concept of the present invention are all within the scope of protection defined by the claims.
Claims (10)
1. A compound extracted from peanut branches and leaves for preventing and treating tetranychus cinnabarinus is characterized in that the compound for preventing and treating tetranychus cinnabarinus comprises palmitic acid, isovanillic acid, β -sitosterol and stigmastane-3, 6-diol;
the palmitic acid has a structure represented by formula (1):
the isovanillic acid has a structure represented by formula (2):
the β -sitosterol has a structure represented by formula (3):
the stigmastane-3, 6-diol has a structure shown in a formula (4):
2. the method for extracting the compound for preventing and treating tetranychus cinnabarinus from peanut branches and leaves as claimed in claim 1, wherein the extraction method comprises one or more of extraction method, thin-plate chromatography, normal-pressure column chromatography, medium-pressure column chromatography and high performance liquid chromatography; the tetranychus cinnabarinus prevention and control compound is extracted from a peanut branch and leaf crude extract, and the peanut branch and leaf crude extract is obtained by soaking and extracting plants in Arachis in leguminosae by using a solvent.
3. The method for extracting tetranychus cinnabarinus-preventing compound from peanut branches and leaves as claimed in claim 2, wherein the thin-plate chromatography is to separate the crude peanut branch and leaf extract on a thin plate laid with silica gel.
4. The method for extracting the compound for preventing and treating tetranychus cinnabarinus from the peanut branches and leaves as claimed in claim 2, wherein the normal pressure column chromatography is to separate the crude extract of the peanut branches and leaves in a glass column with silica gel as a stationary phase.
5. The method for extracting the compound for preventing and treating tetranychus cinnabarinus from peanut branches and leaves as claimed in claim 2, wherein the medium-pressure column chromatography is to separate the substances separated by the normal-pressure column chromatography in a glass column with a fixed phase of C18.
6. The method for extracting tetranychus cinnabarinus-controlling compound from peanut branches and leaves as claimed in claim 2, wherein the high performance liquid chromatography is to put the medium pressure column chromatography separated substance into a chromatographic column for high pressure separation.
7. The method for extracting tetranychus cinnabarinus-preventing compound from peanut branches and leaves as claimed in claim 2, wherein the solvent is one or more of petroleum ether, ethyl acetate, n-butanol, methanol, chloroform and water.
8. The method for extracting tetranychus cinnabarinus-controlling compounds from peanut branches and leaves as claimed in claim 2 or 7, wherein the ratio of petroleum ether to ethyl acetate is 1:0-0:1, the ratio of ethyl acetate to methanol is 1:0-0:1, the ratio of n-butanol to water is 2:1, the ratio of methanol to water is 1:0-0:1, and the ratio of chloroform to water is 1:0-0: 1.
9. The use method of the tetranychus cinnabarinus controlling compound extracted from peanut branches and leaves as claimed in claim 1, which comprises the following steps:
(1) preparing: inoculating 30-60 heads of tetranychus cinnabarinus into the leaves;
(2) soaking, namely soaking the leaves in the tetranychus cinnabarinus preventing and controlling compound with the concentration of 0.5-2mg/m L for 5s, and taking out the leaves;
(3) cleaning: putting the taken-out leaves into clear water, and repeating for 3 times;
(4) counting: and (3) culturing the tetranychus cinnabarinus on the cleaned leaves under normal conditions, and counting the death rate after 24 hours.
10. The method for preparing the acaricide containing compounds for preventing and treating tetranychus cinnabarinus, which is extracted from peanut branches and leaves, as claimed in claim 1, wherein four of the tetranychus cinnabarinus preventing and treating compounds are mixed with an auxiliary agent, wherein the auxiliary agent is one or more of tween 80, N-dimethyldecanamide, methyl oleate and methyl oleate emulsifier.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010489359.5A CN111493099B (en) | 2020-06-02 | 2020-06-02 | Compound extracted from peanut branches and leaves and used for preventing and treating tetranychus cinnabarinus and application of compound |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010489359.5A CN111493099B (en) | 2020-06-02 | 2020-06-02 | Compound extracted from peanut branches and leaves and used for preventing and treating tetranychus cinnabarinus and application of compound |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111493099A true CN111493099A (en) | 2020-08-07 |
| CN111493099B CN111493099B (en) | 2022-01-25 |
Family
ID=71878714
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010489359.5A Active CN111493099B (en) | 2020-06-02 | 2020-06-02 | Compound extracted from peanut branches and leaves and used for preventing and treating tetranychus cinnabarinus and application of compound |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111493099B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101167452A (en) * | 2006-10-23 | 2008-04-30 | 黄永 | Double effective fatty acid preparation for improving plant growing and protecting plant |
| CN101263830A (en) * | 2008-04-30 | 2008-09-17 | 浙江林学院 | Multi-effect pesticidal preparations and uses thereof |
| CN101642103A (en) * | 2008-08-07 | 2010-02-10 | 黄永 | Long-acting insect expelling carrier |
| EP2695517A1 (en) * | 2012-08-09 | 2014-02-12 | Universität Hohenheim | Pheromone composition for treating Varroa mite infestation |
| CN105638657A (en) * | 2015-01-30 | 2016-06-08 | 中国烟草总公司湖南省公司 | Insect and bacterium killing agent, preparation method and application method thereof |
| CN108902195A (en) * | 2018-06-29 | 2018-11-30 | 上海交通大学 | A kind of botanical acaricides and its preparation method and application for preventing and treating Tetranychus cinnabarinus |
| CN109362788A (en) * | 2018-11-29 | 2019-02-22 | 生物源生物技术（深圳）股份有限公司 | A kind of graininess anti-mite agent and its application |
-
2020
- 2020-06-02 CN CN202010489359.5A patent/CN111493099B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101167452A (en) * | 2006-10-23 | 2008-04-30 | 黄永 | Double effective fatty acid preparation for improving plant growing and protecting plant |
| CN101263830A (en) * | 2008-04-30 | 2008-09-17 | 浙江林学院 | Multi-effect pesticidal preparations and uses thereof |
| CN101642103A (en) * | 2008-08-07 | 2010-02-10 | 黄永 | Long-acting insect expelling carrier |
| EP2695517A1 (en) * | 2012-08-09 | 2014-02-12 | Universität Hohenheim | Pheromone composition for treating Varroa mite infestation |
| CN105638657A (en) * | 2015-01-30 | 2016-06-08 | 中国烟草总公司湖南省公司 | Insect and bacterium killing agent, preparation method and application method thereof |
| CN108902195A (en) * | 2018-06-29 | 2018-11-30 | 上海交通大学 | A kind of botanical acaricides and its preparation method and application for preventing and treating Tetranychus cinnabarinus |
| CN109362788A (en) * | 2018-11-29 | 2019-02-22 | 生物源生物技术（深圳）股份有限公司 | A kind of graininess anti-mite agent and its application |
Non-Patent Citations (2)
| Title |
|---|
| YA‐YA LIU，: ""Effect of acaricidal compound extracted from Arachis hypogaea Linn against Tetranychus cinnabarinus"", 《JOURNAL OF APPLIED ENTOMOLOGY》 * |
| 王翘楚，: "《花生枝叶治失眠：20年研究结硕果》", 31 May 2009, 上海科学技术文献出版社 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111493099B (en) | 2022-01-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Muller et al. | Association patterns involving desert plants that contain toxic products | |
| CN104855412A (en) | Dispersible oil suspending agent containing spirotetramat and dinotefuran | |
| CN102175487B (en) | In-situ collection device for plant root secretions and application thereof | |
| CN108033858B (en) | Special clay soil conditioner for facility agriculture | |
| CN102754595A (en) | Huperzia serrata hairy root system preparation and cultivation method | |
| CN107094571A (en) | The implantation methods of Pogostemon cablin seedling under a kind of Continuous Mono-cropping | |
| CN111493099B (en) | Compound extracted from peanut branches and leaves and used for preventing and treating tetranychus cinnabarinus and application of compound | |
| CN113207535A (en) | Seedling raising method for bruguiera gymnorrhiza | |
| CN112219866A (en) | Plant extract pesticide | |
| CN101107901A (en) | Application of coronary toxin in restraining seed germination and the simple preprocessing method of coronary toxin biological detecting method thereof | |
| CN102617530B (en) | Compound extracted from Italian oriental cocklebur and application thereof as weedicide | |
| CN104894206A (en) | Method for enriching huperzine A through huperzia serrata cell and endophytic fungi coculture | |
| CN112471179B (en) | Application of filicina sibirica in pest and mite killing, biological control agent and preparation method thereof | |
| CN109793019B (en) | Plant source repellent for Holotrichia parallela and preparation method thereof | |
| CN102224831B (en) | Control method of tetranychus cinnabarinus based on tomato extracts | |
| CN102763662A (en) | Medium for separating Peronophythora Litchi Chen ex Ko et al | |
| Rao | The insecticidal property of petals of several common plants of India | |
| CN104807925A (en) | Method for extracting and separating allelopathic autotoxicity substances from panax notoginseng rhizosphere soil | |
| CN107372633B (en) | Application of artemisia annua tobacco vinegar liquid in inhibition of root-knot nematodes | |
| CN112106789A (en) | Application of delphinium ethyl acetate extract in preventing and treating diamond back moth | |
| CN101313676B (en) | Biology coating agent, preparation and application in control of flue-cured tobacco axillary bud thereof | |
| KR0155032B1 (en) | Rehmannia glutinosa extract and a safener composition having safener activity to a herbicide paraquat | |
| CN110313476B (en) | Method for preventing and controlling adult willow Blueleaf beetles and repellent used by method | |
| CN116554184B (en) | Novel sesquiterpene alkaloid compound and preparation method and application thereof | |
| CN108840792A (en) | A kind of mapping isopimarane type diterpene and its preparation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |