CN109350589B

CN109350589B - Chinese medicinal hair nourishing and hair growth promoting liquid containing Chinese angelica extract and preparation method thereof

Info

Publication number: CN109350589B
Application number: CN201811502797.XA
Authority: CN
Inventors: 顾锦文; 张沪青; 张菁菁
Original assignee: Shanghai Fenghua Daily Necessities Co ltd
Current assignee: Shanghai Fenghua Daily Necessities Co ltd
Priority date: 2018-12-10
Filing date: 2018-12-10
Publication date: 2021-08-17
Anticipated expiration: 2038-12-10
Also published as: CN109350589A

Abstract

The application relates to a Chinese medicinal hair nourishing and growing liquid containing Chinese angelica extract, which is characterized by comprising the following effective components extracted from Chinese medicinal raw materials: an angelica extract; radix Sophorae Flavescentis extract; cacumen Platycladi extract; a red sage root extract; rhizoma Ligustici Chuanxiong extract; a ginseng extract; a polygonum multiflorum extract; rhizoma Gastrodiae extract; a mulberry extract; and a solvent. The application also relates to a method for preparing the Chinese medicinal hair nourishing and growing liquid containing the angelica sinensis extract. The traditional Chinese medicine hair nourishing and growing liquid mainly reflects the effect of Chinese angelica on inhibiting '5 alpha-reductase', can improve the blood microcirculation of scalp and improve the activity of hair follicle cells through the mutual synergistic effect of nine active substances of traditional Chinese medicines, and has the effects of nourishing and growing hair. In addition, the traditional Chinese medicine hair nourishing and growing liquid can be used for treating male seborrheic alopecia, alopecia areata, alopecia totalis and other crowds, is safe and non-irritant, and is convenient to prepare and use; can strengthen the roots and strengthen the hair, and effectively reduce the hair loss.

Description

Chinese medicinal hair nourishing and hair growth promoting liquid containing Chinese angelica extract and preparation method thereof

Technical Field

The application relates to the technical field of daily chemical industry and washing and caring, in particular to a traditional Chinese medicine hair nourishing and growing liquid containing angelica sinensis extract and a preparation method thereof.

Background

Each person has approximately 100,000-.

The normal hair loss is the hair in the catagen and telogen phases, and the normal amount of hair can be maintained because the hair entering the catagen phase and the hair newly entering the anagen phase are constantly in dynamic balance. Pathological alopecia refers to abnormal or excessive hair loss, and the symptoms of the hair loss are greasy hair, scorched hair, lack of luster, difficult hair loss due to fixation of yellowish scales or flying grey scales, and spontaneous pruritus. Male pattern alopecia, mainly refers to the condition that the hair in the front and top of the head, the hair line and temples on the forehead move upwards, the hair in the front and top is sparse, yellow and soft, and finally the top of the forehead is bald or has a certain amount of hair; female hair loss is at the top of the head, and the hair becomes thin but does not fall off completely in pieces.

The causes of alopecia are various, such as excessive secretion of male hormone, excessive secretion of seborrheic substances, scalp blood circulation disorder, scalp function loss due to peroxidation, bacteria and the like, stress, genetic factors and the like; among them, seborrheic alopecia is the most common, accounting for more than 95% of patients; in addition, in modern society, the population suffering from alopecia is moving toward the young, and the number of the population suffering from alopecia tends to increase.

In the existing technology for treating alopecia in the market, some medicines are added to achieve a certain alopecia prevention effect, for example, western medicines use hormone as an active ingredient to promote blood circulation and strengthen hair root functions; 5 α -reductase inhibitors; contains active substances such as western medicine minoxidil and the like, but the western medicine has certain side effects on a human body, and can cause certain body dysfunction after long-term administration.

In hair follicle cells, testosterone is catalyzed by 5 α -reductase and converted to more biologically active Dihydrotestosterone (DHT), which competes with testosterone for the same receptor, but has 5-fold the activity of dihydrotestosterone. DHT binds to the Androgen Receptor (AR) in the hair follicle, and this particular binding triggers cellular processes that interfere with the anagen phase of the hair cycle, where the hair enters the telogen phase of the hair prematurely. The middle hair is gradually converted into vellus hair, the hair follicle is rudimentated, and the hair coverage is reduced, so that the inhibition of 5 alpha-reductase has positive significance for promoting hair growth. Common western medicines use finasteride as a 5 alpha-reductase inhibitor, but the side effect of the finasteride is reported to be easily caused by sexual dysfunction.

In addition, blood circulation is also very important for hair growth. In the anagen phase, the blood vessels around the hair follicle are richer than those in the telogen phase, and the capillary vessels around the hair papilla are increased and form a network during the growth process, so that sufficient nutrition is provided for the hair. It has been reported in the literature that a decrease in scalp elasticity or a decrease in peripheral vascular blood flow of scalp subcutaneous tissue may be responsible for the disturbance of hair growth. The clinically common alopecia phenomenon is also directly related to the decrease in the number of capillaries and the decrease in the number of hair follicles due to unbalanced nutrition. The clinical antihypertensive effect of the minoxidil for long-term use can achieve the effect of promoting hair growth; however, the long-term use of minoxidil causes certain dependence on the drug, and after the drug is stopped, the hair can be removed again, namely, the minoxidil falling period is entered.

Therefore, the development of a traditional Chinese medicine hair nourishing and hair growing liquid which can inhibit 5 alpha-reductase and does not produce side effects is urgently needed in the field.

Disclosure of Invention

The application aims to provide the Chinese medicinal hair nourishing and growing liquid containing the Chinese angelica extract, which has the advantages of good anti-hair loss effect, difficult relapse after stopping taking the medicine, no side effect and simple preparation process, thereby solving the technical problems in the prior art. Specifically, the application relates to a traditional Chinese medicine hair nourishing and growing liquid composition containing an angelica sinensis extract, which can improve the microcirculation of the scalp, inhibit the effect of '5 alpha-reductase' and improve the activity of hair follicle cells through the mutual synergistic effect of nine active substances, thereby achieving the effects of nourishing and growing hair.

In order to solve the above technical problem, the present application provides the following technical solutions:

in a first aspect, the present application provides a Chinese medicinal hair nourishing and growing liquid containing angelica sinensis extract, which is characterized in that the Chinese medicinal hair nourishing and growing liquid contains the following effective components extracted from Chinese medicinal raw materials:

an angelica extract;

radix Sophorae Flavescentis extract;

cacumen Platycladi extract;

a red sage root extract;

rhizoma Ligustici Chuanxiong extract;

a ginseng extract;

a polygonum multiflorum extract;

rhizoma Gastrodiae extract;

a mulberry extract; and

a solvent.

In one embodiment of the first aspect, the traditional Chinese medicine hair nourishing and health promoting liquid comprises the following effective components extracted from traditional Chinese medicine raw materials, based on the total weight of the traditional Chinese medicine hair nourishing and health promoting liquid as 100%:

0.15-0.25% of Angelica gigas nakai extract;

0.3-0.4% of radix Sophorae Flavescentis extract;

0.1-0.15% of cacumen Platycladi extract;

0.15-0.25% of Salvia miltiorrhiza Bunge extract;

0.15-0.25% of rhizoma Ligustici Chuanxiong extract;

0.15-0.25% of ginseng extract;

0.15-0.25% of Polygonum multiflorum extract;

0.12-0.18% rhizoma Gastrodiae extract;

0.12-0.18% of mulberry extract; and

the balance of solvent.

In one embodiment of the first aspect, the solvent is a mixture of ethanol and water in a free volume ratio of 1:3 to 1: 6.

In one embodiment of the first aspect, the traditional Chinese medicine hair nourishing and growing liquid comprises effective components extracted from the following traditional Chinese medicine raw materials in parts by weight: 10-100 parts of angelica, 10-80 parts of radix sophorae flavescentis, 30-100 parts of cacumen biotae, 10-80 parts of salvia miltiorrhiza, 10-80 parts of ligusticum wallichii, 10-80 parts of ginseng, 10-100 parts of polygonum multiflorum, 10-60 parts of gastrodia elata and 10-60 parts of mulberry;

the extraction method comprises the steps of mixing traditional Chinese medicine raw materials in a preset weight part to obtain a traditional Chinese medicine raw material mixture, and then extracting the traditional Chinese medicine raw material mixture by using a mixture of ethanol and water in a free volume ratio of 1: 3-1: 6 through a decoction method.

In one embodiment of the first aspect, the traditional Chinese medicine hair nourishing and growing liquid comprises effective components extracted from the following traditional Chinese medicine raw materials in parts by weight: 20-100 parts of angelica, 20-80 parts of radix sophorae flavescentis, 30-100 parts of cacumen biotae, 10-80 parts of salvia miltiorrhiza, 20-80 parts of ligusticum wallichii, 20-80 parts of ginseng, 10-100 parts of polygonum multiflorum, 10-60 parts of gastrodia elata and 10-60 parts of mulberry; the extraction method comprises the steps of mixing traditional Chinese medicine raw materials in a preset weight part to obtain a traditional Chinese medicine raw material mixture, and then extracting the traditional Chinese medicine raw material mixture by using a mixture of ethanol and water in a free volume ratio of 1: 3-1: 6 through a decoction method.

In one embodiment of the first aspect, the traditional Chinese medicine hair nourishing and growing liquid comprises effective components extracted from the following traditional Chinese medicine raw materials in parts by weight: 40-60 parts of angelica, 30-50 parts of radix sophorae flavescentis, 30-50 parts of cacumen biotae, 20-60 parts of salvia miltiorrhiza, 20-60 parts of ligusticum wallichii, 30-50 parts of ginseng, 30-50 parts of polygonum multiflorum, 15-30 parts of gastrodia elata and 20-30 parts of mulberry; the extraction method comprises the steps of mixing traditional Chinese medicine raw materials in a preset weight part to obtain a traditional Chinese medicine raw material mixture, and then extracting the traditional Chinese medicine raw material mixture by using a mixture of ethanol and water in a free volume ratio of 1: 3-1: 6 through a decoction method.

In one embodiment of the first aspect, the traditional Chinese medicine hair nourishing and growing liquid comprises effective components extracted from the following traditional Chinese medicine raw materials in parts by weight: 20 parts of angelica, 30 parts of sophora flavescens, 30 parts of cacumen biotae, 20 parts of salvia miltiorrhiza, 20 parts of ligusticum wallichii, 20 parts of ginseng, 20 parts of polygonum multiflorum, 15 parts of gastrodia elata and 15 parts of mulberry; the extraction method comprises the steps of mixing traditional Chinese medicine raw materials in a preset weight part to obtain a traditional Chinese medicine raw material mixture, and then extracting the traditional Chinese medicine raw material mixture by using a mixture of ethanol and water in a free volume ratio of 1: 3-1: 6 through a decoction method.

In a second aspect, the present application provides a method for preparing the Chinese herbal hair-nourishing and hair-growing liquid containing angelica sinensis extract according to the first aspect, wherein the method comprises dividing the Chinese herbal materials into a plurality of groups according to suitable extraction methods for effective ingredients in each Chinese herbal material, extracting the effective ingredients of each group of Chinese herbal materials by using suitable extraction methods, and mixing the effective ingredients extracted from each group to prepare the Chinese herbal hair-growing liquid; wherein each group of Chinese medicinal materials comprises at least one Chinese medicinal material.

In one embodiment of the second aspect, the extraction method comprises one or more of decoction, ultrasonic extraction, microwave-assisted extraction, water extraction and alcohol precipitation, maceration, reflux, percolation or ethanol extraction.

In a third aspect, the present application provides a method for preparing the Chinese herbal hair nourishing and restoring liquid containing angelica sinensis extract according to the first aspect, wherein the method comprises mixing a predetermined weight part of Chinese herbal medicine raw materials to obtain a Chinese herbal medicine raw material mixture, extracting effective components in the Chinese herbal medicine raw material mixture by using a suitable extraction method, and obtaining the Chinese herbal hair nourishing and restoring liquid after removing impurities to the maximum extent.

Compared with the prior art, the traditional Chinese medicine hair nourishing and growing liquid provided by the invention has the beneficial effects that the effect of inhibiting the 5 alpha-reductase of the angelica is mainly reflected, and the scalp blood microcirculation can be improved and the activity of hair follicle cells can be improved through the mutual synergistic effect of nine traditional Chinese medicine active substances, so that the traditional Chinese medicine hair nourishing and growing liquid has the effects of nourishing and growing hair. In addition, the traditional Chinese medicine hair nourishing and growing liquid can be used for treating male seborrheic alopecia, alopecia areata, alopecia totalis and other crowds, is safe and non-irritant, and is convenient to prepare and use; can strengthen the roots and strengthen the hair, and effectively reduce the hair loss.

Drawings

The present application may be better understood by describing embodiments thereof in conjunction with the following drawings, in which:

FIG. 1 shows hair follicle cells of mice cultured in normal cell culture medium for 20 days;

FIG. 2 shows mouse hair follicle cells in culture after 20 days of continuous androgenic nandrolone (dihydrotestosterone DHT);

FIG. 3 shows mouse hair follicle cells in a culture solution after 10 days of continuous addition of a hair tonic according to an embodiment of the present invention;

FIG. 4 shows mouse hair follicle cells in a culture solution after 20 days of continuous addition of a hair tonic according to an embodiment of the present invention;

fig. 5 shows the hair growth on the forehead part before using the hair tonic according to one embodiment of the present invention for hair tonic in example 1;

FIG. 6 shows the hair growth on the forehead region after 30 days using the hair tonic and hair tonic liquid according to one embodiment of the present invention in example 1;

fig. 7 shows the growth of hair on the forehead area before using the hair tonic according to one embodiment of the present invention in example 2;

FIG. 8 shows the hair growth on the forehead area after 20 days using the hair tonic and hair tonic according to one embodiment of the present invention in example 2;

FIG. 9 shows the condition of hair growth on the top of the head before using the hair tonic according to one embodiment of the present invention in example 3; and

fig. 10 shows the hair growth on the top of the head of example 3 after 20 days using the hair tonic and hair tonic according to one embodiment of the present invention.

Detailed Description

In one embodiment, the present application relates to a Chinese medicinal hair nourishing and growing liquid containing angelica sinensis extract. The composition of the invention comprises the following components in percentage by weight: a. comprises 0.15-0.25 wt% of radix Angelicae sinensis extract; b. comprises 0.3-0.4 wt% of radix Sophorae Flavescentis extract; c. comprises cacumen Platycladi extract 0.1-0.15 wt% of hair caring and growing liquid; d. contains Saviae Miltiorrhizae radix extract 0.15-0.25 wt% of hair nourishing and promoting hair growth; e. contains rhizoma Ligustici Chuanxiong extract 0.15-0.25 wt% of hair caring and hair growth promoting liquid; f. contains ginseng extract 0.15-0.25 wt% of hair nourishing and growing liquid; g. contains Polygoni Multiflori radix extract 0.15-0.25 wt% of hair nourishing and promoting solution; h. contains rhizoma Gastrodiae extract 0.12-0.18% of hair nourishing and hair growth promoting liquid; the i contains mulberry extract accounting for 0.12-0.18% of the hair nourishing and growing liquid. The traditional Chinese medicine hair nourishing and growing liquid can improve the blood microcirculation of scalp, inhibit the effect of 5 alpha-reductase and improve the activity of hair follicle cells through the mutual synergistic effect of the nine active substances of the traditional Chinese medicines, and has more reasonable and more effective hair nourishing and growing effects compared with a single active ingredient.

In a specific embodiment, the application provides a traditional Chinese medicine hair nourishing and growing liquid containing angelica sinensis extract, which is characterized by comprising the following active ingredients in parts by weight: 10-100 parts of angelica, 10-80 parts of radix sophorae flavescentis, 30-100 parts of cacumen biotae, 10-80 parts of salvia miltiorrhiza, 10-80 parts of ligusticum wallichii, 10-80 parts of ginseng, 10-100 parts of polygonum multiflorum, 10-60 parts of gastrodia elata and 10-60 parts of mulberry.

In a specific embodiment, the hair nourishing and hair growth promoting liquid comprises the following raw material effective components in parts by weight: 20-100 parts of angelica, 20-80 parts of radix sophorae flavescentis, 30-100 parts of cacumen biotae, 10-80 parts of salvia miltiorrhiza, 20-80 parts of ligusticum wallichii, 20-80 parts of ginseng, 10-100 parts of polygonum multiflorum, 10-60 parts of gastrodia elata and 10-60 parts of mulberry.

In a specific embodiment, the hair nourishing and growing liquid consists of the following raw materials in parts by weight: 40-60 parts of angelica, 30-50 parts of radix sophorae flavescentis, 30-50 parts of cacumen biotae, 20-60 parts of salvia miltiorrhiza, 20-60 parts of ligusticum wallichii, 30-50 parts of ginseng, 30-50 parts of polygonum multiflorum, 15-30 parts of gastrodia elata and 20-30 parts of mulberry.

In a specific embodiment, the hair nourishing and growing liquid consists of the following raw materials in parts by weight: 20 parts of angelica, 30 parts of sophora flavescens, 30 parts of cacumen biotae, 20 parts of salvia miltiorrhiza, 20 parts of ligusticum wallichii, 20 parts of ginseng, 20 parts of polygonum multiflorum, 15 parts of gastrodia elata and 15 parts of mulberry.

In one embodiment, the present application provides a method for preparing a traditional Chinese medicine hair nourishing and growing liquid, which is characterized in that raw materials are divided into a plurality of groups according to the properties of active ingredients in the raw materials, the active ingredients of the raw materials in each group are respectively extracted, and then the extracted active ingredients in each group are mixed to prepare the hair nourishing and growing liquid, wherein each group comprises at least one raw material; or simultaneously extracting the effective components of all the mixed raw materials to prepare the hair nourishing and growing liquid.

In a specific embodiment, the Chinese medicinal hair nourishing and growing liquid containing the Chinese angelica extract comprises the following effective components in parts by weight: 10-100 parts of angelica, 10-80 parts of radix sophorae flavescentis, 30-100 parts of cacumen biotae, 10-80 parts of salvia miltiorrhiza, 10-80 parts of ligusticum wallichii, 10-80 parts of ginseng, 10-100 parts of polygonum multiflorum, 10-60 parts of gastrodia elata and 10-60 parts of mulberry.

In the raw materials for nourishing and growing hair in the technical scheme, the angelica has the function of inhibiting 5 alpha-reductase causing alopecia, enriches blood and activates blood, contains various hydrocarbon substances and vitamin B substances, and provides rich nutrition for hair follicles; the radix Sophorae Flavescentis can inhibit the action of '5 alpha-reductase', clear heat and dry dampness, reduce blood lipid and dredge collaterals, and promote hair growth and hair care; the Chinese medicinal dictionary mentions that the Chinese arborvitae twig has the functions of cooling blood and stopping bleeding, is used for treating alopecia, and can be used for preventing hair from being dense and losing after long-term use; the red sage root has the functions of benefiting qi, strengthening body, resisting oxidation and aging, and growing and nourishing hair; rhizoma Ligustici Chuanxiong has effects of promoting blood circulation, activating qi-flowing, and promoting scalp blood circulation; ginseng radix has effects of invigorating primordial qi, invigorating spleen, and tranquilizing; the polygonum multiflorum has the functions of supporting yang, benefiting yin, blackening hair and growing hair; rhizoma Gastrodiae has effects in calming liver, invigorating kidney, resisting inflammation, and promoting hair growth; mulberry has the functions of enriching blood, nourishing yin, promoting the production of body fluid and moistening dryness, and according to the record in Yunnan herbal medicine, "benefit kidney and consolidate essence, blacken hair and improve eyesight after long-term use". The traditional Chinese medicine components of the invention have synergistic effect with each other, can inhibit the effect of '5 alpha-reductase', improve the blood microcirculation of scalp, activate hair follicle, nourish scalp, strengthen hair root, and achieve the effects of strengthening root, strengthening hair, nourishing hair and growing hair. All the raw materials are directly purchased from a pharmacy, namely, processed dry medicinal materials. The content of the active ingredients of each raw material is experimentally determined, and the good effect is shown in the formula range.

In the technical scheme, as the chemical components of the raw materials in the hair nourishing and growing liquid are complex, and contain various active ingredients and ineffective ingredients, the active ingredients need to be extracted to achieve better hair loss prevention and growing effects. When the effective components of the hair nourishing and hair restoring liquid are extracted, the following modes can be adopted: dividing the raw materials into a plurality of groups according to the properties of the effective components in each group, respectively extracting the effective components of the raw materials in each group, respectively extracting all the components by using a method (such as decoction method, ultrasonic extraction method and microwave-assisted extraction method), and mixing the effective components extracted from each group to obtain the hair nourishing and hair restoring liquid, wherein each group contains at least one raw material; or simultaneously extracting the effective components of all the mixed raw materials to prepare the hair nourishing and growing liquid.

The preferable extraction methods for extracting the effective components of the raw materials respectively are listed below, wherein the extraction methods suitable for the effective components of the angelica sinensis comprise an ethanol reflux extraction method, a water extraction and alcohol precipitation method, an ultrasonic extraction method and a decoction method; the extraction method suitable for radix Sophorae Flavescentis includes ethanol reflux extraction, water extraction and ethanol precipitation, and decocting; the extraction method suitable for cacumen Platycladi comprises ethanol soaking, supercritical CO2 extraction, and decocting; suitable extraction methods for Saviae Miltiorrhizae radix include ultrasonic extraction method and reflux method; suitable for rhizoma Ligustici Chuanxiong extraction methods include ethanol extraction, enzyme extraction, and decocting; the extraction method suitable for Ginseng radix comprises ethanol reflux extraction, ultrasonic extraction, and decocting; the extraction method suitable for Polygoni Multiflori radix includes ethanol extraction, reflux method, and decocting method; the extraction method suitable for rhizoma Gastrodiae comprises Soxhlet extraction, organic solvent extraction, and decocting; the extraction method suitable for the mulberry comprises a percolation method and an alcohol extraction method. Summarizing the extraction methods of the raw materials of the traditional Chinese medicines, some methods are simultaneously suitable for extracting several raw materials, so that the methods can be combined to simultaneously extract the active ingredients of various raw materials so as to improve the extraction efficiency.

After extracting the effective components of the traditional Chinese medicine raw materials of the hair-nourishing and hair-growing liquid, the separation technology is generally adopted to separate the traditional Chinese medicine extract from dregs, precipitates, solid impurities and solvent impurities, thereby removing the ineffective components to the maximum extent and keeping the effective components and auxiliary components. The separation method of the invention comprises the following steps: centrifugal separation, settling separation, filtration separation, and distillation cooling.

As a further optimization of the technical scheme, the hair nourishing and growing liquid is prepared from the following raw materials in parts by weight: 10-100 parts of angelica, 10-80 parts of radix sophorae flavescentis, 30-100 parts of cacumen biotae, 10-80 parts of salvia miltiorrhiza, 10-80 parts of ligusticum wallichii, 10-80 parts of ginseng, 10-100 parts of polygonum multiflorum, 10-60 parts of gastrodia elata and 10-60 parts of mulberry. When the weight of the raw materials of the hair nourishing and growing liquid is within the range, the hair strengthening and growing effect is most obvious, and the number of fallen hairs can be reduced. As a further improvement to the above technical scheme, the hair nourishing and growing liquid comprises the following active ingredients in parts by weight: 40 parts of angelica, 40 parts of sophora flavescens, 50 parts of cacumen biotae, 60 parts of salvia miltiorrhiza, 60 parts of ligusticum wallichii, 50 parts of ginseng, 50 parts of polygonum multiflorum, 30 parts of gastrodia elata and 30 parts of mulberry. The obtained hair nourishing and growing liquid has a good hair-fixing effect, and the test example shows that the effective rate of preventing alopecia and growing hair is 58%.

As a further optimization of the technical scheme, the hair nourishing and growing liquid comprises the following active ingredients in parts by weight: 20-100 parts of angelica, 20-80 parts of radix sophorae flavescentis, 30-100 parts of cacumen biotae, 10-80 parts of salvia miltiorrhiza, 20-80 parts of ligusticum wallichii, 20-80 parts of ginseng, 10-100 parts of polygonum multiflorum, 10-60 parts of gastrodia elata and 10-60 parts of mulberry. When the weight of the raw materials of the hair nourishing and growing liquid is within the range, the hair strengthening and growing effect is most obvious, and the number of fallen hairs can be reduced. As a further improvement to the above technical scheme, the hair nourishing and growing liquid comprises the following active ingredients in parts by weight: 60 parts of angelica, 50 parts of sophora flavescens, 50 parts of cacumen biotae, 60 parts of salvia miltiorrhiza, 60 parts of ligusticum wallichii, 50 parts of ginseng, 50 parts of polygonum multiflorum, 30 parts of gastrodia elata and 30 parts of mulberry. The obtained hair nourishing and growing liquid has a good hair-fixing effect, and test examples show that the effective rate of preventing alopecia and growing hair is 63%.

As a further optimization of the technical scheme, the hair nourishing and growing liquid comprises the following active ingredients in parts by weight: 40-60 parts of angelica, 30-50 parts of radix sophorae flavescentis, 30-50 parts of cacumen biotae, 20-60 parts of salvia miltiorrhiza, 20-60 parts of ligusticum wallichii, 30-50 parts of ginseng, 30-50 parts of polygonum multiflorum, 15-30 parts of gastrodia elata and 20-30 parts of mulberry. When the weight of the raw materials of the hair nourishing and growing liquid is within the range, the hair strengthening and growing effect is most obvious, and the number of fallen hairs can be reduced. As a further improvement to the above technical scheme, the hair nourishing and growing liquid comprises the following active ingredients in parts by weight: 40 parts of angelica, 40 parts of sophora flavescens, 40 parts of cacumen biotae, 30 parts of salvia miltiorrhiza, 30 parts of ligusticum wallichii, 40 parts of ginseng, 30 parts of polygonum multiflorum, 20 parts of gastrodia elata and 20 parts of mulberry. The obtained hair nourishing and growing liquid can stop alopecia, strengthen hair and promote hair follicle growth, and experimental examples show that the effective rate of hair stopping and growing is 71%.

As a further optimization of the technical scheme, the hair nourishing and growing liquid comprises the following active ingredients in parts by weight: 20 parts of angelica, 30 parts of sophora flavescens, 30 parts of cacumen biotae, 20 parts of salvia miltiorrhiza, 20 parts of ligusticum wallichii, 20 parts of ginseng, 20 parts of polygonum multiflorum, 15 parts of gastrodia elata and 15 parts of mulberry. The obtained hair nourishing and growing liquid has obvious hair loss prevention and growing effects, the newly grown hair is strong and tough, and the test example shows that the effective rate of hair loss prevention and growing is 79%.

The invention also provides a preparation method of the hair nourishing and growing liquid, which comprises the steps of dividing the raw materials into a plurality of groups according to the properties of the effective components in the raw materials, respectively extracting the effective components of each group, and then mixing the effective components; or simultaneously extracting the effective components of all the mixed raw materials to prepare the hair nourishing and growing liquid.

As a further improvement to the above technical solution, the method for preparing the hair nourishing and growing liquid comprises: the effective components of all the mixed raw materials are extracted by a decoction method and an ethanol extraction method.

As a further improvement to the above technical solution, the method for preparing the hair tonic and hair tonic solution comprises the steps of:

1) weighing 1 part of the compound traditional Chinese medicine (kushen root: cacumen biotae: root of red-rooted salvia: rhizoma ligustici wallichii root: the root of the Chinese angelica is: ginseng root: root of Polygonum multiflorum Thunb: rhizoma gastrodiae: mulberry fruit), cleaning, filtering, drying, crushing, and sieving with a 20-30 mesh sieve; adding a mixed reagent which is 10-20 times of the weight of the traditional Chinese medicine powder and consists of ethanol and water with a free volume ratio of 1: 3-1: 6, decocting for 12-24 hours, filtering to obtain a filtrate, and recovering ethanol for later use;

2) sterilizing the filtrate prepared in the step 1), and subpackaging to obtain the hair nourishing and growing liquid.

In one embodiment, the application method of the Chinese herbal hair nourishing and growing liquid containing angelica sinensis extract is to apply the hair nourishing and growing liquid to scalp once in the morning and once in the evening, and 10 days are 1 experiment period.

Examples

The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. The reagents and raw materials used are commercially available unless otherwise specified. The experimental methods without specifying specific conditions in the following examples were selected according to the conventional methods and conditions, or according to the commercial instructions.

Preparation examples

Example 1

A traditional Chinese medicine hair nourishing and growing liquid containing angelica sinensis extract comprises the following effective components in parts by weight: 40 parts of angelica, 40 parts of sophora flavescens, 50 parts of cacumen biotae, 60 parts of salvia miltiorrhiza, 60 parts of ligusticum wallichii, 50 parts of ginseng, 50 parts of polygonum multiflorum, 30 parts of gastrodia elata and 30 parts of mulberry.

The preparation method of the traditional Chinese medicine hair nourishing and growing liquid comprises the following steps:

weighing 1 part of the compound traditional Chinese medicine (the root of the Sophora flavescens ait: cacumen biotae: radix salviae miltiorrhizae: rhizoma ligustici wallichii: radix angelicae: radix ginseng: radix polygoni multiflori: rhizoma gastrodiae: mulberry fruit), cleaning, filtering, drying, crushing, and sieving with a 20-30-mesh sieve; adding a mixed reagent which is 10-20 times of the weight of the traditional Chinese medicine powder and consists of ethanol and water in a free volume ratio of 1: 3-1: 6, decocting for 12-24 hours, filtering to obtain a filtrate, recovering ethanol, sterilizing, and packaging to obtain the hair nourishing and hair restoring liquid.

Example 2

A traditional Chinese medicine hair nourishing and growing liquid containing angelica sinensis extract comprises the following effective components in parts by weight: 60 parts of angelica, 50 parts of sophora flavescens, 50 parts of cacumen biotae, 60 parts of salvia miltiorrhiza, 60 parts of ligusticum wallichii, 50 parts of ginseng, 50 parts of polygonum multiflorum, 30 parts of gastrodia elata and 30 parts of mulberry.

The preparation method of the traditional Chinese medicine hair nourishing and growing liquid of the embodiment is the same as the embodiment 1.

Example 3

A traditional Chinese medicine hair nourishing and growing liquid containing angelica sinensis extract comprises the following effective components in parts by weight: 40 parts of angelica, 40 parts of sophora flavescens, 40 parts of cacumen biotae, 30 parts of salvia miltiorrhiza, 30 parts of ligusticum wallichii, 40 parts of ginseng, 30 parts of polygonum multiflorum, 20 parts of gastrodia elata and 20 parts of mulberry.

Example 4

A traditional Chinese medicine hair nourishing and growing liquid containing angelica sinensis extract comprises the following effective components in parts by weight: 20 parts of angelica, 30 parts of sophora flavescens, 30 parts of cacumen biotae, 20 parts of salvia miltiorrhiza, 20 parts of ligusticum wallichii, 20 parts of ginseng, 20 parts of polygonum multiflorum, 15 parts of gastrodia elata and 15 parts of mulberry.

Effects of the embodiment

C57BL6 mouse assay

The experimental conditions for the mouse experiments were as follows:

the microscope model was an XSP-13CC biomicroscope, available from Shanghai Chuikang optical instruments, Inc.

The experimental mouse culture equipment is a cell incubator (Heracell VIOS 250i, siemer feishel). The cell culture conditions were as follows: temperature (37 ℃), CO₂Level (5%), pH value (pH value: 7.2-7.4), relative saturation humidity (95%).

The mouse cells are C57BL6 mouse back hair follicle cells. The cell culture solution is prepared by adding 2mmol/L of glutamine into a William culture medium, 2mmol/L of Hepes, 10ng/mL of hydrocortisone, 10ug/mL of bovine insulin, 10ug/mL of transferrin, 10ug/mL of sodium selenite, 100U/mL of penicillin and 100ug/mL of streptomycin.

Volume of mouse experimental cell culture solution: 48 well plates, volume 140 ul/well, initial concentration of hair follicle cells: 1.2*10⁵And (4) cells.

The efficacy of the traditional Chinese medicine hair nourishing and growing liquid according to example 4 for growing hair was evaluated by a C57BL6 mouse experiment: taking 24C 57BL6 mice, removing the back hair of the mice with the age of 30-42 days, stripping hair follicle cells, taking 1ul of an androgynolone (dihydrotestosterone DHT) or 1ul of a hair nourishing and hair growing liquid sample, respectively adding the samples into a cell culture solution, respectively adding the samples once in the morning and at night, continuously culturing for 20 days, recording the hair follicle cells of the mice by using a high-power microscope (microscope model: XSP-13CC biomicroscope, Shanghai Chuikang optical instrument Co., Ltd.) every 10 days, and observing the condition of new hair growth of the hair follicles.

Mouse hair growth results see fig. 1-4, where fig. 1 shows mouse hair follicle cells cultured in normal cell culture medium for 20 days; FIG. 2 shows mouse hair follicle cells in culture after 20 days of continuous feeding of androgynous dragon; FIG. 3 shows the mouse hair follicle cells in the culture solution after 10 days of continuous addition of the hair tonic and hair tonic of the present invention; FIG. 4 shows the mouse hair follicle cells in the culture solution 20 days after the hair tonic and hair tonic of the present invention was continuously added. As can be seen from FIGS. 1 to 4, the hair nourishing and hair restoring liquid of the present invention can effectively inhibit the 5 alpha-reductase, fine hair grows from hair follicle cells after 10 days, the hair follicle cells increase the number of hairs and the hair diameter becomes thicker after 20 days; it has obvious effects of fixing and growing hair.

Experiment of individuals

The Chinese herbal hair tonic according to example 4, which contained the angelica sinensis extract, was used in each of example 1(35 years old, female), example 2(42 years old, male) and example 3(30 years old, male) which were selected. The application method comprises applying the hair nourishing and growing liquid on scalp once in the morning and once in the evening, and taking 10 days as 1 experimental period. The state of the hair was observed by a scalp Microscope (Portable 50-500X 2MP Light Digital Microscope, Shenzhen Fei Takejiu Co., Ltd.).

FIGS. 5 and 6 are graphs for comparing effects of the hair tonic and hair tonic of the present invention on each of examples 1 before and after 30 days in individual test examples; in which fig. 5 shows the growth of hair on the forehead area before use and fig. 6 shows the growth of hair on the forehead area after use, it is apparent that no hair is present before use and that new hair grows after use, and that the vellus hair becomes thick and strong before use.

FIGS. 7 and 8 are graphs for comparing the effects of the hair tonic and hair tonic of the present invention on individual test cases 2 before and after 20 days; wherein fig. 7 shows the hair growth on the forehead area before use, and fig. 8 shows the hair growth on the forehead area after use, it is clearly seen that the hair loss effect is improved, and the hair roots are firm and the hair becomes black and tough after use, and the hair is thin and soft before use.

FIGS. 9 and 10 are graphs for comparing the effects of the hair tonic and hair tonic of the present invention on individual test cases 3 before and after 20 days; wherein, fig. 9 shows the hair growth condition of the top of the head before use, fig. 10 shows the hair growth condition of the top of the head after use, and obviously, 1 to 2 hairs grow out from the first 1 hair follicles, 2 to 3 hairs grow out from the last 1 hair follicles, the hair diameter becomes thicker, and new hairs grow out from the original hair-free positions.

Population test example:

the first step, 96 groups of alopecia areata, alopecia totalis and alopecia in the age range of 22-52 years (alopecia areata 30, alopecia totalis 24 and alopecia totalis 42) are selected as trial objects; 56 men and 40 women; randomly dividing the multiple-choice testers into a first group, a second group, a third group and four groups, wherein each group comprises 24 people, and the first group, the second group, the third group and the four groups respectively try the hair nourishing and hair restoring liquid prepared in the example 1, the example 2, the example 3 and the example 4, and the using method comprises the following steps: applying hair nourishing hair growth liquid on scalp, and using for 3 experimental periods (30 days in total) once a day in the morning and at night;

secondly, the evaluation standard of the curative effect is as follows: a) the method is remarkably effective: the hair loss is obviously improved, the number of the hair loss and breakage is obviously reduced, hair grows obviously in a hairless area, and the hair roots are firm and tough; b) the method has the following advantages: the hair loss is improved, the number of broken hairs is reduced, vellus hairs grow without hairs, but the vellus hairs are not firm enough and break when pulled; c) no effect is obtained: the alopecia phenomenon is not improved and reduced, and no hair can grow in hairless areas.

Specific data are shown in table 1:

the embodiments described above are intended to facilitate the understanding and appreciation of the application by those skilled in the art. It will be readily apparent to those skilled in the art that various modifications to these embodiments may be made, and the generic principles described herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present application is not limited to the embodiments herein, and those skilled in the art who have the benefit of this disclosure will appreciate that many modifications and variations are possible within the scope of the present application without departing from the scope and spirit of the present application.

Claims

1. The traditional Chinese medicine hair nourishing and growing liquid is characterized in that the effective components of the traditional Chinese medicine hair nourishing and growing liquid are extracted from the following traditional Chinese medicine raw materials in parts by weight: 10-100 parts of angelica, 10-80 parts of radix sophorae flavescentis, 30-100 parts of cacumen biotae, 10-80 parts of salvia miltiorrhiza, 10-80 parts of ligusticum wallichii, 10-80 parts of ginseng, 10-100 parts of polygonum multiflorum, 10-60 parts of gastrodia elata and 10-60 parts of mulberry;

2. The Chinese herbal hair nourishing and growing liquid containing the angelica sinensis extract as claimed in claim 1, wherein the effective components of the Chinese herbal hair nourishing and growing liquid are extracted from the following Chinese herbal raw materials in parts by weight: 20-100 parts of angelica, 20-80 parts of radix sophorae flavescentis, 30-100 parts of cacumen biotae, 10-80 parts of salvia miltiorrhiza, 20-80 parts of ligusticum wallichii, 20-80 parts of ginseng, 10-100 parts of polygonum multiflorum, 10-60 parts of gastrodia elata and 10-60 parts of mulberry.

3. The Chinese herbal hair nourishing and growing liquid containing the angelica sinensis extract as claimed in claim 1, wherein the effective components of the Chinese herbal hair nourishing and growing liquid are extracted from the following Chinese herbal raw materials in parts by weight: 40-60 parts of angelica, 30-50 parts of radix sophorae flavescentis, 30-50 parts of cacumen biotae, 20-60 parts of salvia miltiorrhiza, 20-60 parts of ligusticum wallichii, 30-50 parts of ginseng, 30-50 parts of polygonum multiflorum, 15-30 parts of gastrodia elata and 20-30 parts of mulberry.

4. The Chinese herbal hair nourishing and growing liquid containing the angelica sinensis extract as claimed in claim 1, wherein the effective components of the Chinese herbal hair nourishing and growing liquid are extracted from the following Chinese herbal raw materials in parts by weight: 20 parts of angelica, 30 parts of sophora flavescens, 30 parts of cacumen biotae, 20 parts of salvia miltiorrhiza, 20 parts of ligusticum wallichii, 20 parts of ginseng, 20 parts of polygonum multiflorum, 15 parts of gastrodia elata and 15 parts of mulberry.

5. A method for preparing the Chinese herbal hair nourishing and growing liquid containing the angelica sinensis extract according to any one of claims 1 to 4, wherein the method comprises the steps of mixing a predetermined weight part of Chinese herbal raw materials to obtain a Chinese herbal raw material mixture, and extracting the Chinese herbal raw material mixture by a decoction method by using a mixture of ethanol and water in a free volume ratio of 1:3 to 1:6 to obtain the Chinese herbal hair nourishing and growing liquid.