CN109336988A - A kind of extracting method of dendrobium candidum - Google Patents
A kind of extracting method of dendrobium candidum Download PDFInfo
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Abstract
The present invention relates to biomedicine technical fields, especially a kind of extracting method of dendrobium candidum, after fresh dendrobium candidum or dry dendrobium candidum water are impregnated, freezing is crushed, is extracted, so that the effective component in dendrobium candidum is significantly retained, and extract, particular avoids to active ingredient loss in dendrobium candidum, the effect of improving dendrobium candidum extracting solution reduces dendrobium candidum effective component and extracts difficulty.
Description
Technical field
The present invention relates to biomedicine technical field, especially a kind of extracting method of dendrobium candidum.
Background technique
Dendrobium candidum (Dendrobium officinale kimura et migo) is the perennial grass of growing nonparasitically upon another plant of orchid family dendrobium nobile
This plant just has the laudatory title of " gold in medicine " since ancient times, civil to be known as " help mesona ".With Herba Saussureae Involueratae, ginseng, the tuber of multiflower knotweed,
Poria cocos, ganoderma lucidum, pearl, cordyceps sinensis etc. are eponymous, are classified as first of " Chinese nine big mesonas ".It records in Shennong's Herbal, " book on Chinese herbal medicine
Lead to profound ", supplementary Amplifications of the Compendium of Materia Medica, " pharmacological property ", " book on Chinese herbal medicine new again ", in " Dictionary of Chinese Pharmacy ", and it is equal to its effect and value
Exact record and elaboration are made.Modern pharmacological research is thought: dendrobium nobile includes active polysaccharide, dendrobium nobile phenanthrene class, dendrobine, organic
The effective components such as the various essential amino acids of selenium, human body, microelement have enriching yin benefit saliva, nourish the liver to improve visual acuity, strengthen the spleen and stomach, strengthening tendons
Lipid-loweringing and other effects.But for how to play the maximum clinical value of dendrobium nobile, save from damage in dendrobium nobile how more scientificly
Effective component, and its effective component is extracted into utilization, become the technical issues of those skilled in the art pay close attention to.
Currently, it is more for the research of dendrobium nobile extracting method, such as: water extract-alcohol precipitation, referring to compound fresh dendrobium stem oral solution system
(aobvious chapter of Huang etc., traditional Chinese medical science forum, 2007,22 (4): the introduction in 45~46), principle are to utilize dendrobium polysaccharide to standby technical study
It is soluble easily in water, it is insoluble in the characteristic of ethyl alcohol, realizes the extraction to dendrobium polysaccharide；But in this process, some protein are dissolved in water,
Also it is extracted, it is subsequent to be difficult to separate, cause extracting solution apt to deteriorate.And in order to improve the recovery rate of polysaccharide, mentioned in water
During alcohol precipitation, needs to add alkali or acid adding: diluted alkaline is added, so that diluted alkaline abolishes the β in cell wall-Isosorbide-5-Nitrae glycosidic bond, so that more
Associative key between sugar and protein disconnects, and then improves the solubility of polysaccharide, still, still along with albumen in extracting solution
The generation of the impurity such as matter causes extraction fluid viscosity larger, filtration difficulty；And it is broken the process of polysaccharide and protein associative key
In, it is easy to cause active constituent to lose, influences the quality for extracting liquid product；Acid is added, realizes the improvement to dendrobium polysaccharide yield,
But the fracture of polysaccharide glycosidic bond in an acidic solution, is easily caused, causes the active constituent in the solution extracted by shadow
It rings；Moreover, in the method, it is also necessary to alcohol (or organic solvent) precipitation processing be added, cause safety in dendrobium nobile extraction process
Property is difficult to be protected, and is easy to pollute the environment.
For this purpose, having researcher on the basis of prior art research, using ultrasonic wave, microwave-assisted, the equal energy of effective component
Accelerate to leach, increases recovery rate, but, ultrasonic wave, microwave are usually applied in the lab, in the industry few application,
Reason is ultrasonic wave, microwave treatment higher cost, and energy consumption is larger；Then, and there is researcher using enzyme auxiliary or super-pressure
Ancillary technique, realizing improves the recovery rate of dendrobium polysaccharide, but enzyme inherently protein, is equivalent to and increases into dendrobium extract
Impurity is added, and the price of enzyme is higher, industrialized production still remains difficulty；Super-pressure ancillary technique, due to equipment investment
It is larger, but cost significantly increases.
Moreover, permanent development has been obtained in current dendrobium nobile extracting method, and there is a large amount of technical literatures, example
Such as: the extracting method of the Dendrobium officinale polysaccharide of Patent No. 201410010334.7, by fresh dendrobium candidum, chopping is placed in -25
DEG C~-20 DEG C freeze 6~8 hours, under room temperature thaw after, impregnated with the organic solvent of 8~20 times of weight, removal impregnate
Liquid obtains the dregs of a decoction；Lye extracts: by the dregs of a decoction by solid and liquid weight than being 0.5%~1% for the addition weight content of 1:10~40
Aqueous slkali extracts, and obtains extracting solution；Concentration: extracting solution is concentrated into the 1/2 of original volume, obtains concentrate；By the concentrate
PH value is adjusted to 9~11, is stood, filtering obtains filtered fluid I；Active carbon is added in the filtered fluid I, filtering obtains filtered fluid II,
It is dry to get.So that the recovery rate of dendrobium polysaccharide can reach 36% or more, and dendrobium polysaccharide content reaches 96% in extract
More than.But this method still uses organic solvent and lye is added to extract, and the method that non-present invention uses.
Again for example: the extraction work of dendrobium polysaccharide and dendrobine in the dendrobium candidum that number of patent application is 201410586453.7
That introduces in skill utilizes polysaccharide and dendrobine in cellulose enzyme process combined extracting dendrobium candidum, obtains while obtaining dendrobium polysaccharide
Dendrobine is obtained, and maintains the single high efficiency for extracting polysaccharide of enzyme process, the influence that can effectively abolish cell wall is provided, promotes more
The dissolution of sugar etc.；But cellulase is used, new impurity is added, polysaccharide quality is caused to be affected.
Again for example: extraction simultaneously prepares polysaccharide, flavones and life in the slave HERBA DENDROBII of Patent No. 201410615012.5
Introduced in the method for alkaloids by extract kettle in, subcritical water carries out Dynamic Extraction to it, inhales on the extracting solution obtained
Attached resin column carries out Dynamic Adsorption, after extracting and adsorbing, elutes adsorption column with the ethanol solution of various concentration respectively, realizes
Extract polysaccharide in dendrobium nobile, alkaloid and flavones simultaneously, but in this process, used along with ethyl alcohol etc., cause safety by
A degree of influence, although realize will extract and separation coupling, its obtain extract in polyoses content,
Alkaloid, flavones content etc. are lower, cause extract product inferior quality.
Again for example: the Dendrobium nobile polysaccharide of Patent No. 201410628418.7 and the extraction separation method of total alkali, be with
Medicinal material HERBA DENDROBII is raw material, is successively waited through crushing, sieving, enzymatic hydrolysis, high-pressure homogeneous, flocculation, Ultra filtration membrane, freeze-drying
Journey maximizes and extracts Dendrobium nobile polysaccharide and total alkali, the use of organic reagent avoided, so that obtained Dendrobium nobile polysaccharide content
>=94%, dendrobium nobile total alkali >=90%.
Again for example: being introduced in the extracting method of the high dendrobium candidum of the recovery rate that number of patent application is 201810048760.8
Crushing, decoction, filtrate bottling freezing, then be freeze-dried, crushed, then be placed in heating and refluxing extraction in thermostat water bath,
It filters while hot, is condensed into thick paste, it is dry to constant weight, so that polysaccharide extract rate reaches 42%.
It to sum up, in the prior art, for dendrobium candidum as in raw material extraction process, is considered dendrobium candidum
Middle dendrobium polysaccharide, dendrobine, dendrobium nobile extracting flavonoids are separated, and the method used is easy to cause the effective component in dendrobium nobile to meet with
It is damaged, especially in crushing process, shreds or crush using room temperature substantially, in the process, high degree
Affect the activity of effective component, and during processing, due to wherein, cause containing the impurity such as protein viscosity compared with
Greatly, separating difficulty is larger, and protein exists, and be easy to cause extraction solution to occur rotten, influences the shelf-life for extracting solution, make
It must be frequently necessary to that the ingredients such as preservative are added thereto, ensure quality cannot.
Summary of the invention
In order to solve the above technical problems existing in the prior art, the present invention provides a kind of extraction side of dendrobium candidum
Method, by the dendrobium candidum containing certain amount of moisture is freezed 8-12h at -20 DEG C~-50 DEG C, preferably -30 DEG C~-45
8-10h is freezed at DEG C, is crushed, realizes low-temperature grinding dendrobium candidum, and the water of 10-30 times of weight of comminuting matter is accounted for using use,
It is extracted at 60-90 DEG C three times, extracts 1-3h every time, mixing obtains extracting solution, and by being warming up to 60-90 DEG C to extracting solution,
It is cooled to≤45 DEG C again, is preferably cooled to the cryogenic temperature carried out to dendrobium candidum, repetitive operation three times, realizes medium temperature, low temperature
Alternately sterilization treatment；Sterile 0.22 μm of filtering with microporous membrane is used again, is concentrated by evaporation, and is realized and is sterile filtered, to the greatest extent
Remain effective component and the protection to effective component in dendrobium candidum.
Using crushing after freezing, so that realizing crushing process at low ambient temperatures, crushing process generation is not only avoided
Destruction of the high temperature to effective component in dendrobium candidum, but also dendrobium candidum cell wall can be allowed to rupture, it is ensured that subsequent
In extraction process, realize dendrobium candidum effective component Fast Stripping, compared to the prior art, first by dendrobium candidum chopping or
Person crushes and then is carried out for freezing processing, more facilitates the dissolution of effective component in dendrobium candidum, especially passes through
Cross fresh dendrobium candidum or adopt dry dendrobium candidum to be soaked in water and handle after 8-12h taking-up drains, then carried out-
Freezing processing 8-12h, preferably 12h at 20 DEG C~-50 DEG C, so that realizing the water in dendrobium candidum cell wall in refrigerating process
Divide and changes, so that promote the rupture of dendrobium candidum cell wall, and the rupture will form cell wall along dendrobium candidum cell wall
Lines rupture, avoids causing cell wall additional damage, avoids the introducing of impurity；And help to mitigate subsequent disintegrating process
Difficulty, realize in crushing process, according to ruptured lines realization be fragmented into end, avoid to dendrobium candidum cell wall
Mechanical damage by a relatively large margin is caused, remains the active component content of effective component in dendrobium candidum to the greatest extent, so that
It after being added using the water for accounting for 10-30 times of material weight, is extracted three times at 60-90 DEG C, and extracts 1-3h according to each, then will be each
The material of extraction mixes, and obtains extracting solution, so that containing a large amount of effective component in the extracting solution, and active constituent is higher.
Especially by using being warming up to 60-90 DEG C to extracting solution, being then cooled to≤room temperature, repetitive operation is three times, real
Medium temperature, low temperature intersection sterilizing are showed, high degree kills microorganism and bacterium, while dendrobium candidum bio protease being gone out
It is living, avoid the rotten of extracting solution storage process；In conjunction with using 0.22 μm of membrane filtration processing, high degree eliminates extracting solution
In bacterium, it is ensured that filter liquor store process keep stablize, especially in entire technical process, it is not necessary that organic solvent is added
Processing, avoids the pollution of organic solvent.
Extracting method in the invention, specifically includes the following steps:
(1) fresh dendrobium candidum is taken, the freezing processing 8-12h at -20 DEG C~-50 DEG C is crushed；
(2) it uses comminuting matter and water according to weight ratio for 1:10-30, is extracted at 60-90 DEG C three times, extract 1- every time
3h will extract solution mixing three times, and obtain extracting solution；
(3) extracting solution is warming up to 60-90 DEG C, then is cooled to≤45 DEG C, repetitive operation three times, then uses sterile 0.22 μm
Filtering with microporous membrane, filtered fluid is concentrated by evaporation, obtain concentrate.
And clinical application for convenience can be by said extracted method in conjunction with preparation technology of preparing in the prior art
The extraction product of acquisition is used to prepare into oral solution, mask and other products, such as: the extraction material obtained will be extracted and the bletilla striata is extracted
After object, Astragalus Root P.E, dried orange peel extracts, licorice etc. directly compound, by it according to facial mask system in the prior art
Preparation Method is prepared into mask product；Again for example: adding directly into extract or do not add acceptable auxiliary material in pharmacy
(such as: improve smell: Radix Glycyrrhizae sucdrol, honey element；Improve sticky: sodium alginate, Arabic gum, gelatin, Methyl cellulose
Element, sodium carboxymethylcellulose etc.) after, oral solution is configured to according to the prior art.
Moreover, this method extract can also add or not add acceptable auxiliary material in pharmacy, according to existing
There is technology preparation method to be prepared into other oral preparations, such as: tablet, capsule, granule, syrup, powder or dry-mixed outstanding
Agent etc..
In the invention compared with the prior art for the technical solution of Patent No. 201410010334.7, use
It is to adopt fresh dendrobium candidum or dry dendrobium candidum after being soaked in water, it is first freezed into 8- at -20 DEG C~-50 DEG C
12h, preferably 12h；It is preferred that freezing 8-10h at -30 DEG C~-45 DEG C；It is crushed again, realizes low-temperature grinding；And the prior art is
Fresh dendrobium candidum is first shredded, then is freezed；It can be seen that: the invention is first to carry out freezing and then mechanical crushing processing；And
The prior art be first chopping and then freezing processing；And first freezing processing crushes again, can high degree avoid mechanical force to iron
Skin dendrobium nobile cell wall causes mechanical damage, so that can rupture trace in crushing process according to frozen cell wall and realize crushing
Fragmentation reduces crushing difficulty, and will not influence the content of active constituent in dendrobium candidum effective component；And first pass through chopping
Or crush, cause mechanical force larger to the active force of dendrobium candidum cell wall, fragmentation caused to need to consume larger energy consumption,
Being easy to appear fever causes the active constituent in effective component to inactivate, and influences the quality for extracting product.
Specific embodiment
It is limited below with reference to specific embodiment technical solution of the present invention is further, but claimed
Range is not only limited to made description.
Polysaccharide determination method:
Polyoses content is measured using Sulphuric acid-anthrone colorimetry, is measured at 620nm wavelength using ultraviolet-spectrophotometer
Absorbance.
Prepare reference substance solution: precision weighs glucose control product 25mg and is placed in 250ml volumetric flask, is dissolved in water and fixed
Hold, 0.1mg/ml is made.
The preparation (ready-to-use) of sulfuric acid-anthrone solution: precision weighs anthrone 0.1g, adds 80% sulfuric acid solution 100ml
Make to dissolve, shake up, sets in brown bottle, it is stored refrigerated.
Draw standard curve: it is accurate respectively to draw that 0.1mg/ml glucose control product solution is appropriate, it is diluted to different dense
Degree, then the accurate each dilute solution and glucose control product solution 2.0ml of measuring, in 10ml tool plug test tube, sulfuric acid-is added in precision
Anthrone solution 6.0ml sets heating water bath 15min, takes out and cools down 20 minutes rapidly, using distilled water as blank control, according to purple
Outside-visible spectrophotometry measures absorbance at 620nm and draws standard curve.
Measurement operation: precision measures extracting solution 2ml made from the invention embodiment, in 10ml tool plug test tube, essence
Close addition sulfuric acid-anthrone solution 6.0ml sets heating water bath 15min, takes out and cools down 20 minutes rapidly, using distilled water as blank
Control measures absorbance according to UV-visible spectrophotometry at 620nm, looks into corresponding standard curve, calculates content.
Dendrobine measuring method:
It is detected using gas chromatography.
Prepare reference substance solution: precision weighs dendrobine 50mg in 100ml volumetric flask, adds methanol constant volume to scale, shakes
It is even；Precision measures 1ml into 10ml volumetric flask, adds methanol constant volume to scale, shakes up, obtain 50 μ g/ml reference substance solutions.
Chromatographic condition: DB-1 capillary column (30mm × 0.25mm, 0.25 μm)；Program is by 80 DEG C, with 10 DEG C/min speed
250 DEG C are warming up to, is kept for 5 minutes；250 DEG C of injector temperature, detect 250 DEG C of its temperature, 10 μ l of sample volume, number of theoretical plate is low
In 1000.
Draw standard curve: it is accurate respectively to draw that 50 μ g/ml dendrobine reference substance solutions are appropriate, it is diluted to different dense
Degree, then each dilute solution sample introduction measurement of accurate measurement, draw standard curve.
Measurement operation: precision measures extracting solution 5ml made from the invention embodiment, surveys according to above-mentioned condition sample introduction
It is fixed, corresponding standard curve is looked into, content is calculated.
In the examples below, filtered fluid evaporation and concentration is the concentration mark according to demands such as oral preparations in the prior art
Standard is concentrated.
Embodiment 1
Fresh dendrobium candidum 100g is taken, in -20 DEG C of freezing 12h, is crushed；It is extracted three times, every time for 70 DEG C again using 2000g water
Extraction time is 2h, is merged, and extracting solution is obtained；Extracting solution is warming up to 80 DEG C, cools to 40 DEG C, repetitive operation three times, then
Using sterile 0.22 μm of filtering with microporous membrane, filtered fluid is concentrated by evaporation, is spray-dried, is prepared into granule.
Embodiment 2
Fresh dendrobium candidum 100g is taken, in -30 DEG C of freezing 10h, is crushed；It is extracted three times, every time with 2500g water at 60 DEG C
2h merges, and obtains extracting solution；Extracting solution is warming up to 75 DEG C, cools to 35 DEG C, repetitive operation three times, then uses sterile
0.22 μm of filtering with microporous membrane, filtered fluid is concentrated by evaporation, and acceptable auxiliary material in the pharmacies such as regulator is added, is prepared into
Oral solution.
Acceptable auxiliary material in the pharmacies such as regulator is added and is prepared into facial mask or Essence.
Embodiment 3
Fresh dendrobium candidum 100g is taken, in -45 DEG C of freezing 8h, is crushed；It is extracted three times with 1000g water at 90 DEG C, each 1h,
Merge, obtains extracting solution；Extracting solution is warming up to 90 DEG C, cools to 30 DEG C, repetitive operation three times, then uses sterile 0.22 μm
Filtering with microporous membrane, filtered fluid is concentrated by evaporation, regulator (such as: bletilla striata extract) is added, is prepared into Essence or face
Film.
Embodiment 4
Fresh dendrobium candidum 100g is taken, in -50 DEG C of freezing 9h, is crushed；It is extracted three times with 3000g water at 70 DEG C, each 3h,
Merge, obtains extracting solution；Extracting solution is warming up to 60 DEG C, cools to 25 DEG C, repetitive operation three times, then uses sterile 0.22 μm
Filtering with microporous membrane, filtered fluid is concentrated by evaporation, regulator (such as: bletilla striata extract) is added, is prepared into facial mask.
Embodiment 5
Fresh dendrobium candidum 200g is taken, be first soaked in water 12h, in -40 DEG C of cryogenic freezing 8h, crushes；With 4000g water 90
DEG C extract 3 times, each 1h, No. 3 extracting solutions are collected together；Extracting solution is warming up to 90 DEG C, 35 DEG C is cooled to, is repeated 3 times
Operation, then with sterile 0.22 μm of filtering with microporous membrane；Filtered fluid is concentrated by evaporation to meeting the requirements.It is added as needed isotonic
Eye drops is made in agent, sterilizing.
Embodiment 6
Take dry dendrobium candidum 80g, be first soaked in water 12 hours, -45 DEG C cryogenic freezing 10 hours, crush；With
3000g water extracts 3 times at 70 DEG C, 1.5 hours every time, No. 3 extracting solutions is collected together；Extracting solution is warming up to 80 DEG C, so
After be cooled to 45 DEG C, operation is repeated 3 times, then with sterile 0.22 μm of filtering with microporous membrane；Filtered fluid is concentrated by evaporation to symbol
It closes and requires.After spray drying, starch, disintegrating agent, lubricant etc. is added, can be made into tablet.
Embodiment 7
Take dry dendrobium candidum 150g, be first soaked in water 12 hours, drain, -45 DEG C cryogenic freezing 12 hours, powder
It is broken；It is extracted 3 times, 1 hour every time, No. 3 extracting solutions is collected together at 80 DEG C with 3000g water；Extracting solution is warming up to 80 DEG C,
40 DEG C are cooled to, operation is repeated 3 times, then with sterile 0.22 μm of filtering with microporous membrane；Filtered fluid is concentrated by evaporation to meeting
It is required that.After spray drying, disintegrating agent, lubricant etc. is added, can be made into oral disintegrating tablet.
Extracting solution prepared by embodiment 1-7 and " compound fresh dendrobium stem oral solution Study on Preparation (aobvious chapter of Huang etc., traditional Chinese medical science
Forum, 2007,22 (4): the extracting solution (control) that the water extraction and alcohol precipitation method 45~46) " introduced in a text extracts is created according to the present invention
The polysaccharide determination method and dendrobine measuring method of making introduction carry out the measurement of polysaccharide, dendrobium nobile alkali content, result such as the following table 1 institute
Show:
Table 1
As shown in Table 1, the invention, which is passed through, adopts the processing that is soaked in water for fresh dendrobium candidum or dry dendrobium candidum
Afterwards, Freezing smashing, then extract；So that it is compared to for traditional water extraction and alcohol precipitation method, polysaccharide extracts about 16% or more more, and yield mentions
It is high by about 89%；Dendrobine extracts about 0.024% more, and yield about improves 75%, it can be seen that, alcohol precipitation is easy to cause in dendrobium candidum
Part loss of effective components, and in the invention, by the powder after Freezing smashing, after being mentioned using water, directly being sterilized makes
With reducing the loss of effective component.
In order to verify the necessity of dendrobium candidum pulverization process under cryogenic, this researcher in the course of the research, is pressed
Processing is extracted as raw material according to the fresh dendrobium candidum in the invention embodiment 5, and cryogenic temperature is adjusted to -40
Under the conditions of DEG C, 10h is freezed, water is added to squeeze the juice；Control is to directly adopt fresh dendrobium candidum to squeeze the juice at normal temperature；Again by above-mentioned two groups
The measurement of polysaccharide and dendrobine, result such as table are carried out according to the dendrobium polysaccharide and dendrobine measuring method of the invention introduction
Shown in 2:
Table 2
| Dendrobium polysaccharide (%) | Dendrobine (%) | |
| Freeze low-temperature treatment juicing | 34.03 | 0.033 |
| Direct room temperature juicing | 24.10 | 0.021 |
As shown in Table 2, it after freezing low-temperature treatment, squeezes the juice compared to direct room temperature and handles the dendrobium candidum juice of acquisition
In, dendrobium polysaccharide content about more 10%, dendrobium nobile alkali content about more 0.012%, it is seen then that for freezing low-temperature treatment, facilitate iron
The dissolution of the effective components such as dendrobium polysaccharide and dendrobine in skin dendrobium nobile；The dissolution is under freezing conditions, so that dendrobium candidum is thin
Cell wall under the conditions of lines according to rupturing, can under mechanical action, according to cell wall rupture lines and in expressing process
The fragmentation for realizing dendrobium candidum cell wall, reduces loss of the mechanical force to effective component in dendrobium candidum, it is more to improve dendrobium nobile
The dissolution of sugar, dendrobine.
Temperature, time and place from the point of view of the data in conjunction with shown in table 1, table 2, during to dendrobium candidum freezing processing
Science and engineering skill, it will the dissolution for influencing effective component in dendrobium candidum of high degree.
In order to guarantee the shelf-life of dendrobium candidum extracting solution, effective component in the reservation dendrobium candidum extracting solution of high degree
Content, this researcher in the course of the research, also to dendrobium candidum extracting solution according to the nothing introduced in 2015 editions " Chinese Pharmacopoeias "
Bacterium detection method is detected, and specific processing method is as follows:
Extracting solution prepared by above-described embodiment 1-7 has sterile growth detection to handle as follows respectively, and processing is will to extract
Liquid is directly detected, and is handled according to the heating, cooling introduced in embodiment 1-7, and after carrying out once, detection one
It is secondary；After carrying out second of heating, cooling processing again, second of detection；After carrying out third time heating, cooling processing again, inspection
Third time is surveyed, testing result is as shown in table 3 below:
Table 3
| Directly detect | Heating and cooling 1 time | Heating and cooling 2 times | Heating and cooling 3 times | |
| Embodiment 1 | There is bacterium growth | There is bacterium growth | There is bacterium growth | Asepsis growth |
| Embodiment 2 | There is bacterium growth | There is bacterium growth | There is bacterium growth | Asepsis growth |
| Embodiment 3 | There is bacterium growth | There is bacterium growth | There is bacterium growth | Asepsis growth |
| Embodiment 4 | There is bacterium growth | There is bacterium growth | There is bacterium growth | Asepsis growth |
| Embodiment 5 | There is bacterium growth | There is bacterium growth | There is bacterium growth | Asepsis growth |
| Embodiment 6 | There is bacterium growth | There is bacterium growth | There is bacterium growth | Asepsis growth |
| Embodiment 7 | There is bacterium growth | There is bacterium growth | There is bacterium growth | Asepsis growth |
As it can be seen that handling by heating and cooling, enable to the protein in extracting solution during medium temperature, low temperature cross processing
Be denaturalized so that extracting solution keep germ-free condition, by heating and cooling 3 times three times processing after so that in extracting solution be in nothing
Bacterium state, the stability for improving extracting solution of high degree, extends the shelf-life of dendrobium candidum extracting solution, avoids to iron
The additives such as preservative are added in skin dendrobium extract, ensure that the quality of dendrobium candidum extracting solution.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (10)
1. a kind of extracting method of dendrobium candidum, which is characterized in that use fresh dendrobium candidum or the dendrobium candidum of drying
The water logging swollen taking-up that is soaked drains, the freezing processing at -20 DEG C~-50 DEG C, and crushing；It is extracted again, obtains extracting solution.
2. the extracting method of dendrobium candidum as described in claim 1, which is characterized in that the extracting solution, using being warming up to
After 60-90 DEG C, then≤45 DEG C are cooled to, and repeated circular treatment at least 3 times, then used sterile 0.22 μm of miillpore filter
Filtering obtains filtrate, and filtrate is concentrated, and obtains concentrate.
3. the extracting method of dendrobium candidum as described in claim 1, which is characterized in that the soaking time is 12h.
4. the extracting method of dendrobium candidum as described in claim 1, which is characterized in that the cooling time is 12h.
5. the extracting method of dendrobium candidum as described in claim 1, which is characterized in that the freezing, temperature are -30 DEG C
~-45 DEG C, time 8-10h.
6. the extracting method of dendrobium candidum as described in claim 1, which is characterized in that the extraction will crush acquisition
Powder is at 60-90 DEG C, to repeat to extract three times after 1:10-30 is mixed according to weight ratio with water, extracts 1-3h every time, and will
Solution mixing is extracted three times, obtains extracting solution.
7. the extracting method of dendrobium candidum as described in claim 1, which is characterized in that specifically includes the following steps:
(1) fresh dendrobium candidum is taken, the freezing processing 8-12h at -20 DEG C~-50 DEG C is crushed；
(2) it uses comminuting matter and water according to weight ratio for 1:10-30, is extracted at 60-90 DEG C three times, extract 1-3h every time,
Solution mixing will be extracted three times, obtain extracting solution；
(3) extracting solution is warming up to 60-90 DEG C, then is cooled to≤45 DEG C, repetitive operation is three times, then micro- using sterile 0.22 μm
Hole membrane filtration, filtered fluid is concentrated by evaporation, and obtains concentrate.
8. the extract as the extracting method of the described in any item dendrobium candidums of claim 1-7 obtains is used to be configured to take orally
Liquid, facial mask.
9. the extract as the extracting method of the described in any item dendrobium candidums of claim 1-7 obtains adds by spray drying
After entering pharmaceutically acceptable auxiliary material, applied to oral preparation is made.
10. application as claimed in claim 9, which is characterized in that the oral preparation is tablet, capsule, granule, syrup
Agent or powder.
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| CN114231382A (en) * | 2021-11-09 | 2022-03-25 | 赤水信天斛满堂药业有限公司 | Dendrobium nobile vinegar, preparation method and device |
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Application publication date: 20190215 |