CN103142682A - Method for extracting liquorice flavonoids from liquorice residue - Google Patents
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Abstract
The invention relates to a method for extracting liquorice flavonoids, and in particular relates to a method for extracting liquorice flavonoids from liquorice residue. The method comprises the following steps of: (1) ultrasonic enzymolysis: drying and grinding the liquorice residue, and performing ultrasonic treatment on a mixed solution of cellulase and pectinase; (2) flocculation purification: adding a chitosan colloidal solution and an aqueous solution of 101 fruit juice clarifying agent into a concentrated solution; and (3) passing through a macroporous resin column: passing the supernate through a D101 resin column, eluting with ethanol with the volume concentration of 50%, concentrating the elution liquid, and drying under reduced pressure to obtain liquorice total flavonoids. The method provided by the invention can be used for obviously improving the extraction rate and purity of liquorice total flavonoids.
Description
Technical field:
The present invention relates to a kind of method of extracting licoflavone, especially relate to a kind of method of extracting licoflavone from glycyrrhiza residue.
Background technology:
Radix Glycyrrhizae is the dry root and rhizome of pulse family glycyrrhiza genus Radix Glycyrrhizae Glycyrrhiza uralensis Fisch, Glycyrrhiza inflata Bat. Glycyrrhiza inflata Bat or Glycyrrhiza glabra L. Glycyrrhiza glabra L.Be rich in triterpene and flavones ingredient, wherein the pentacyclic triterpenoid glycyrrhizic acid is one of main effective ingredient.The methods such as medicine manufacture often adopts that water extraction, moisture alcohol extraction, alkali are carried are extracted glycyrrhizic acid from licorice medicinal materials, after extracting, remaining liquorice dregs discards as industrial waste usually.Further studies show that and be rich in a large amount of flavone compounds in liquorice dregs, these flavones ingredients have biological activity widely, as antibacterial, antifungal, enhancing human body immunity function, anti-H IV, antitumor, antiulcer, antioxidation, protect the liver and blood sugar lowering etc.Thereby liquorice dregs is a kind of recycling resource of preciousness, and it can be widely used in the fields such as medicine, food and cosmetics.
At present more to the Study on extraction of glycyrrhizic acid, also comparative maturity.But study for the extraction process of flavones ingredient in Radix Glycyrrhizae less, utilize to extract waste residue after glycyrrhizic acid extract licoflavone still less.The classical extracting method of licoflavone has solvent extraction, polycaprolactam method etc. to extract licoflavone, because complex process, yield is low, and the high in cost of production reason is not suitable for suitability for industrialized production.For extract this commercial production waste material of liquorice dregs that obtains after Radix Glycyrrhizae extractum and glycyrrhizic acid from Radix Glycyrrhizae; if taken full advantage of; excavate its medical value and economic worth; be conducive to solve the difficult problem that industrial production refuse is processed; more fully rationally utilize the Radix Glycyrrhizae plant resources and preserve the ecological environment, surely can produce huge benefit.
The effective ingredient of plant often is wrapped in cell wall, and the enzyme facture can be hydrolyzed the destruction cell wall structure, and effective ingredient is directly come out, and dissolving, suspendible or peptization are in extracting solvent, to improve the extraction ratio of total flavones.Enzymatic isolation method have reaction temperature and, high, with low cost, the simple operation and other advantages of yield.Hyperacoustic cavitation can be accelerated plant cell wall and break, and promotes the release of active substance, and extraction efficiency is high, and the time is short, and impurity is few, is widely used in the extraction of active components of plants.The modern crafts of above-mentioned enzymolysis and ultrasonication have been introduced in the technical scheme of extracting the plant total flavones, but remain the deficiency that haves much room for improvement at some.
Summary of the invention
Technical problem to be solved by this invention is to provide the extracting method of Radix Glycyrrhizae total flavones in a kind of improved glycyrrhiza residue, and the method has significantly improved extraction ratio and the purity of Radix Glycyrrhizae total flavones.
The technical scheme that the present invention addresses the above problem is as follows:
A kind of method of extracting licoflavone from glycyrrhiza residue is comprised of following steps:
(1) ultrasonic enzymolysis: get drying, to add mass concentration be 0.05%～0.15% composite enzyme solution to the glycyrrhiza residue after pulverizing, addition is 10～30ml composite enzyme solution/1g glycyrrhiza residue, pH value with solution transfers to 4.5～6 again, then under 40～60 ℃ with 50KHZ, the 160W supersound process is warming up to rapidly 90 ℃ after 0.5～1.5 hour, keep the 10min enzyme denaturing, filter, collect filtrate, be evaporated to relative density and be 1.05 concentrated solution, in the time of 60 ℃; Wherein, described compound enzyme is the mixture of cellulase and pectase, and quality proportioning both is 1:1;
(2) flocculation remove impurity: under 60 ℃ of conditions, first the ratio in 80mL chitosan gum liquid solution/1000mL concentrated solution adds chitosan in concentrated solution, fully stir, ratio in 80mL 101 fruit juice clarifier aqueous solution/1000mL concentrated solutions adds 101 fruit juice clarifier aqueous solutions again, fully stir, hold over night, supernatant is got in centrifugalize; Wherein, described chitosan gum liquid solution is that volumetric concentration is that chitosan mass concentration that 1% acetum and chitosan are mixed is 1% acetum, described 101 fruit juice clarifier aqueous solutions be water and 101 fruit juice clarifiers be mixed 101 fruit juice clarifier mass concentrations be 5% aqueous solution;
(3) cross macroporous resin column: supernatant is crossed the D101 resin column, is 50% ethanol elution with volumetric concentration, and eluent is concentrated, and drying under reduced pressure namely gets Radix Glycyrrhizae total flavones.
As the preferred version of technique scheme, the addition of the described composite enzyme solution of step (1) is 20ml composite enzyme solution/1g glycyrrhiza residue.
As the preferred version of technique scheme, the mass concentration of the described composite enzyme solution of step (1) is 1%.
As the preferred version of technique scheme, the pH value of the solution that the described glycyrrhiza residue of step (1) mixes with composite enzyme solution transfers to 5.0.
As the preferred version of technique scheme, the temperature of the described supersound process of step (1) is 50 ℃, and the time is 1 hour.
The inventive method has following advantages than prior art:
(1) well-known, mainly contain cellulose and lignin in glycyrrhiza residue, contain simultaneously pectic substance; Cellulose, lignin and pectic substance are the different materials of two class features, and the Degradation of different enzymes is not identical.Compound enzyme of the present invention just is being based on above-mentioned factor and is designing, utilize cellulase fully degrade cellulose and lignin in glycyrrhiza residue, utilize fully degrade wherein pectic substance of pectase, simultaneously in conjunction with hyperacoustic effect, activity that can kinase can be accelerated the stripping of flavone again, thereby the extraction ratio of Radix Glycyrrhizae total flavones is significantly improved.
(2) due to glycyrrhiza residue through after ultrasonic enzymolysis, have the macromole unstability impurity such as the larger particle of granule, heavy metal ion in extracting solution and have a strong impact on purity and the quality of extract.Therefore, the present invention's removal step of flocculating first adds chitosan, utilize its adsorption bridging and to the neutralization of negative charge, remove particle etc. in Chinese medicine extraction liquid, then add 101 fruit juice clarifiers, utilize the dual function of absorption and the coacervation of 101 fruit juice clarifiers, make the quick coacervation precipitation of described macromole impurity; Simultaneously, described 101 fruit juice clarifiers have absorption and floculation too to the residual chitosan in solution, have further eliminated residual chitosan to the impact of extract quality.This technology is applied to the remove impurity of flavones ingredient, and the yield of Radix Glycyrrhizae total flavones is significantly promoted, and compares the extraction process of not using this flocculation removal step, and yield can improve 5 times of left and right.
(3) in addition, the method for the invention also have advantages of process operation simple, consuming time less, cost is low and safety non-toxic.
Description of drawings
Fig. 1 is the liquirtin standard curve.
The specific embodiment
The present invention will be described in detail below in conjunction with specific embodiment and Comparative Examples, and embodiment is only the preferred embodiment of the present invention, is not limitation of the invention.
Embodiment 1
1, extract Radix Glycyrrhizae total flavones
(1) first do the preparation in early stage, that is, that glycyrrhiza residue is dry and be ground into coarse powder; Getting the cellulase of equivalent and pectase adds entry to be prepared into concentration is 0.1% composite enzyme solution; Get chitosan 0.8g, join 80mL concentration and be that to be made into chitosan concentration in 1% acetum be 1% chitosan gum liquid solution, standby; Get 101 fruit juice clarifier 4g, joining and being made into 101 juice clarification agent concentrations in the 80mL distilled water is 5% 101 fruit juice clarifier aqueous solutions, standby.
(2) extracting liquorice slag coarse powder 1000g, add in the composite enzyme solution of preparation, addition is 1g glycyrrhiza residue/20ml composite enzyme solution, again the pH value of solution is transferred to 5.0, then is warming up to rapidly 90 ℃ after ultrasonic (50KHZ, 160W) under 50 ℃ processes 1.0h, keep the 10min enzyme denaturing, filter, it is 1.05(60 ℃ that filtrate decompression is concentrated into relative density), obtain concentrated solution.
(3) under condition, first add chitosan gum liquid solution 80mL in the 1000ml of previous step gained concentrated solution in the time of 60 ℃, fully stir, then add 101 fruit juice clarifier aqueous solution 80mL, fully stir, hold over night, supernatant is got in centrifugalize.
(4) supernatant being crossed the D101 macroporous resin column, is 50% ethanol elution with volumetric concentration, and eluent is concentrated, and drying under reduced pressure is weighed, and gets Radix Glycyrrhizae total flavones crude product 32g.
2, the purity testing of Radix Glycyrrhizae total flavones
(1) preparation of reference substance solution: precision takes liquirtin reference substance 5.08mg and puts in the 5ml volumetric flask, adds dissolve with methanol and is diluted to scale, and making concentration is 1.016mg ﹒ ml
-1The reference substance stock solution.The accurate 1ml reference substance solution of drawing adds methanol constant volume to scale in the 10ml volumetric flask, obtaining concentration is 0.1016mg ﹒ ml
-1Reference substance solution stand-by.
(2) drafting of standard curve: the accurate 0.1016 mg ﹒ ml that draws
-1Liquirtin reference substance solution 0.4,0.6,0.8,1.0,1.2,1.4 m1 put respectively in the 10ml volumetric flask, add 10% KOH solution 0.5ml, after placing 5min, be diluted to scale with methanol, with corresponding solvent as blank solution.Measure absorption spectrum between wavelength 250～600 nm, maximum absorption wavelength be record respectively each volumetric flask under the 337nm condition in the absorbance of reference substance.The value take the concentration of reference substance as abscissa (X), absorbance is vertical coordinate (Y) value, draws out the coordinate of 6 corresponding point in rectangular coordinate system, then carrying out linear regression, to obtain correlation coefficient be that the standard curve of r=0.9997 is Y=0.018+3.3824x.Standard curve is seen Fig. 1.
(3) assay of total flavones in sample: precision takes Radix Glycyrrhizae total flavones crude product 20mg and is placed in the 25mL measuring bottle, adds concentration and be 50% ethanol standardize solution, filters; Precision measures subsequent filtrate 1mL and is placed in the 10ml measuring bottle, adds concentration and be 50% ethanol standardize solution; Then precision measures 0.5ml from the measuring bottle of 10ml, and by the described method of above-mentioned steps (2), adds 10%KOH solution, then obtain testing sample and measure absorbance (this routine measurement result is 0.486) with methanol constant volume to 10 ml; At last, with measured absorbance substitution equation Y=0.018+3.3824x, (amount that contains total flavones in this routine gained 32g Radix Glycyrrhizae total flavones crude product is [(0.486-0.018)/3.3824] * 0.5 * 10 * 25 * 32000/20=27672.6(mg), namely gets the content of total flavones in the Radix Glycyrrhizae total flavones crude product to calculate the amount of total flavones in testing sample according to the preparation process of testing sample again.The general flavone content of this routine gained 22g Radix Glycyrrhizae total flavones crude product is 27.672 ÷ 32 * 100%=86.5%.
Embodiment 2
(1) glycyrrhiza residue is dry and be ground into coarse powder; Getting the cellulase of equivalent and pectase adds entry to be prepared into concentration is 0.15% composite enzyme solution; Making chitosan concentration by the identical method of example 1 is that 1% chitosan gum liquid solution and 101 juice clarification agent concentrations are 5% 101 fruit juice clarifier aqueous solutions, standby.
(2) extracting liquorice slag coarse powder 1000g, add in the composite enzyme solution of preparation, addition is 1g glycyrrhiza residue/25ml composite enzyme solution, again the pH value of solution is transferred to 4.5, then is warming up to rapidly 90 ℃ after ultrasonic (50KHZ, 160W) under 40 ℃ processes 1.0h, keep the 10min enzyme denaturing, filter, it is 1.05(60 ℃ that filtrate decompression is concentrated into relative density), obtain concentrated solution.
(3) under condition, first add chitosan gum liquid solution 80mL in previous step gained 1000ml concentrated solution in the time of 60 ℃, fully stir, then add 101 fruit juice clarifier aqueous solution 80mL, fully stir, hold over night, supernatant is got in centrifugalize.
(4) supernatant is crossed the D101 macroporous resin column, it is 50% ethanol elution with volumetric concentration, eluent is concentrated, drying under reduced pressure, weigh, get Radix Glycyrrhizae total flavones crude product 27g, press the method for detecting purity of embodiment 1 described Radix Glycyrrhizae total flavones, the general flavone content that records gained 27g Radix Glycyrrhizae total flavones crude product is 73.0%.
Embodiment 3
(1) glycyrrhiza residue is dry and be ground into coarse powder; Getting the cellulase of equivalent and pectase adds entry to be prepared into concentration is 0.05% composite enzyme solution; Making chitosan concentration by the identical method of embodiment 1 is that 1% chitosan gum liquid solution and 101 juice clarification agent concentrations are 5% 101 fruit juice clarifier aqueous solutions, standby.
(2) extracting liquorice slag coarse powder 1000g, add in the composite enzyme solution of preparation, addition is 1g glycyrrhiza residue/20ml composite enzyme solution, again the pH value of solution is transferred to 6.0, then is warming up to rapidly 90 ℃ after ultrasonic (50KHZ, 160W) under 60 ℃ processes 1.5h, keep the 10min enzyme denaturing, filter, it is 1.05(60 ℃ that filtrate decompression is concentrated into relative density), obtain concentrated solution.
(3) under condition, first add chitosan gum liquid solution 80mL in previous step gained 1000ml concentrated solution in the time of 60 ℃, fully stir, then add 101 fruit juice clarifier aqueous solution 80mL, fully stir, hold over night, supernatant is got in centrifugalize.
(4) supernatant is crossed the D101 macroporous resin column, it is 50% ethanol elution with volumetric concentration, eluent is concentrated, drying under reduced pressure, weigh, get Radix Glycyrrhizae total flavones crude product 28g, press the method for detecting purity of embodiment 1 described Radix Glycyrrhizae total flavones, the general flavone content that records gained 30g Radix Glycyrrhizae total flavones crude product is 75.7%.
Comparative example 1
(1) glycyrrhiza residue is dry and be ground into coarse powder; Getting the cellulase of equivalent and pectase adds entry to be prepared into concentration is 0.1% composite enzyme solution.
(2) extracting liquorice slag coarse powder 1000g, the composite enzyme solution that adds the previous step preparation, addition is 1g glycyrrhiza residue/10ml composite enzyme solution, again the pH value of solution is transferred to 5.0, then be warming up to rapidly 90 ℃ after ultrasonic (50KHZ, 160W) under 50 ℃ processes 1.0h, keep the 10min enzyme denaturing, filter, it is 1.05(60 ℃ that filtrate decompression is concentrated into relative density).
(3) add appropriate ethanol, make to contain alcohol amount and reach 60～80%, standing cold preservation 12 hours is got supernatant and reclaimed ethanol, and is concentrated, filters, and gets supernatant.
(4) supernatant is crossed the D101 macroporous resin column, it is 50% ethanol elution with volumetric concentration, eluent is concentrated, drying under reduced pressure, weigh, get Radix Glycyrrhizae total flavones crude product 15g, press the method for detecting purity of embodiment 1 described Radix Glycyrrhizae total flavones, the general flavone content that records gained 15g Radix Glycyrrhizae total flavones crude product is 40.5%.
As seen this comparative example is compared with embodiment 1, and the step of ultrasonic enzymolysis and purification by macroporous resin is identical, and the remove impurity of just flocculating is used the ethanol precipitation instead and removed impurity, and the purity of gained crude product just obviously reduces.
Comparative example 2
(1) glycyrrhiza residue is dry and be ground into coarse powder; Getting the cellulase of equivalent and pectase adds entry to be prepared into concentration is 0.1% composite enzyme solution; Making chitosan concentration by the identical method of embodiment 1 is 1% chitosan gum liquid solution, standby.
(2) extracting liquorice slag coarse powder 1000g, the composite enzyme solution that adds the previous step preparation, addition is 1g glycyrrhiza residue/15ml composite enzyme solution, again the pH value of solution is transferred to 5.0, then be warming up to rapidly 90 ℃ after ultrasonic (50KHZ, 160W) under 50 ℃ processes 1.0h, keep the 10min enzyme denaturing, filter, it is 1.05(60 ℃ that filtrate decompression is concentrated into relative density).
(3) under condition, add chitosan gum liquid solution 80mL in the time of 60 ℃, fully stir, hold over night, supernatant is got in centrifugalize.
(4) supernatant is crossed the D101 macroporous resin column, it is 50% ethanol elution with volumetric concentration, eluent is concentrated, drying under reduced pressure, weigh, get Radix Glycyrrhizae total flavones crude product 19g, by the method for detecting purity of example 1 described Radix Glycyrrhizae total flavones, the general flavone content that records gained 19g Radix Glycyrrhizae total flavones crude product is 51.3%.
As seen this comparative example is compared with embodiment 1, and the step of ultrasonic enzymolysis and purification by macroporous resin is identical, and the step of 101 fruit juice clarifier clarifications in the removal step of just flocculating is omitted, and the purity of gained crude product also obviously reduces.
Comparative example 3
(1) glycyrrhiza residue is dry and be ground into coarse powder; Getting the cellulase of equivalent and pectase adds entry to be prepared into concentration is 0.1% composite enzyme solution.Making 101 juice clarification agent concentrations by the identical method of embodiment 1 is 5% 101 fruit juice clarifier aqueous solutions, standby.
(2) extracting liquorice slag coarse powder 1000g, the composite enzyme solution that adds the previous step preparation, addition is 1g glycyrrhiza residue/10ml composite enzyme solution, again the pH value of solution is transferred to 5.0, then be warming up to rapidly 90 ℃ after ultrasonic (50KHZ, 160W) under 50 ℃ processes 1.0h, keep the 10min enzyme denaturing, filter, it is 1.05(60 ℃ that filtrate decompression is concentrated into relative density).
(3) under condition, add 101 fruit juice clarifier aqueous solution 80mL in the time of 60 ℃, fully stir, hold over night, supernatant is got in centrifugalize.
(4) supernatant is crossed the D101 macroporous resin column, it is 50% ethanol elution with volumetric concentration, eluent is concentrated, drying under reduced pressure, weigh, get Radix Glycyrrhizae total flavones crude product 21g, press the method for detecting purity of embodiment 1 described Radix Glycyrrhizae total flavones, the general flavone content that records gained 21g Radix Glycyrrhizae total flavones crude product is 56.8%.
As seen this comparative example is compared with embodiment 1, and the step of ultrasonic enzymolysis and purification by macroporous resin is identical, and the step of the chitosan clarification in the removal step of just flocculating is omitted, and the purity of gained crude product also obviously reduces.
Comparative example 4
(1) glycyrrhiza residue is dry and be ground into coarse powder; The extracting cellulose enzyme adds entry, and to be prepared into concentration be 0.1% enzymatic solution; Making chitosan concentration by the identical method of example 1 is that 1% chitosan gum liquid solution and 101 juice clarification agent concentrations are 5% 101 fruit juice clarifier aqueous solutions, standby.
(2) extracting liquorice slag coarse powder 1000g, the enzymatic solution that adds the previous step preparation, addition is 1g glycyrrhiza residue/10ml enzymatic solution, again the pH value of solution is transferred to 5.0, then be warming up to rapidly 90 ℃ after ultrasonic (50KHZ, 160W) under 50 ℃ processes 1.0h, keep the 10min enzyme denaturing, filter, it is 1.05(60 ℃ that filtrate decompression is concentrated into relative density).
(3) under condition, first add chitosan gum liquid solution 80mL in the time of 60 ℃, fully stir, then add 101 fruit juice clarifier aqueous solution 80mL, fully stir, hold over night, supernatant is got in centrifugalize.
(4) supernatant is crossed the D101 macroporous resin column, it is 50% ethanol elution with volumetric concentration, eluent is concentrated, drying under reduced pressure, weigh, get Radix Glycyrrhizae total flavones crude product 14g, by the method for detecting purity of routine described Radix Glycyrrhizae total flavones, the general flavone content that records gained 14g Radix Glycyrrhizae total flavones crude product is 37.8%.
As seen this comparative example is compared with embodiment 1, and the step of flocculation remove impurity and purification by macroporous resin is identical, just uses the compound enzyme in ultrasonic enzymolysis step instead single cellulase, and the yield of Radix Glycyrrhizae total flavones just obviously reduces.
Comparative example 5
(1) glycyrrhiza residue is dry and be ground into coarse powder; Getting pectase adds entry to be prepared into concentration is 0.1% enzymatic solution; Making chitosan concentration by the identical method of example 1 is that 1% chitosan gum liquid solution and 101 juice clarification agent concentrations are 5% 101 fruit juice clarifier aqueous solutions, standby.
(2) extracting liquorice slag coarse powder 1000g, the enzymatic solution that adds the previous step preparation, addition is 1g glycyrrhiza residue/15ml enzymatic solution, again the pH value of solution is transferred to 5.0, then be warming up to rapidly 90 ℃ after ultrasonic (50KHZ, 160W) under 50 ℃ processes 1.0h, keep the 10min enzyme denaturing, filter, it is 1.12(60 ℃ that filtrate decompression is concentrated into relative density).
(3) under condition, first add chitosan gum liquid solution 80mL in the time of 60 ℃, fully stir, then add 101 fruit juice clarifier aqueous solution 80mL, fully stir, hold over night, supernatant is got in centrifugalize.
(4) supernatant is crossed the D101 macroporous resin column, it is 50% ethanol elution with volumetric concentration, eluent is concentrated, drying under reduced pressure, weigh, get Radix Glycyrrhizae total flavones crude product 10g, press the method for detecting purity of embodiment 1 described Radix Glycyrrhizae total flavones, the general flavone content that records gained 10g Radix Glycyrrhizae total flavones crude product is 60%.。
As seen this comparative example is compared with embodiment 1, and the step of flocculation remove impurity and purification by macroporous resin is identical, just uses the compound enzyme in ultrasonic enzymolysis step instead single pectase, and the yield of Radix Glycyrrhizae total flavones also obviously reduces.
Claims (5)
1. a method of extracting licoflavone from glycyrrhiza residue, is characterized in that, the method is comprised of following steps:
(1) ultrasonic enzymolysis: get drying, to add mass concentration be 0.05%～0.15% composite enzyme solution to the glycyrrhiza residue after pulverizing, addition is 10～30ml composite enzyme solution/1g glycyrrhiza residue, pH value with solution transfers to 4.5～6 again, then under 40～60 ℃ with 50KHZ, the 160W supersound process is warming up to rapidly 90 ℃ after 0.5～1.5 hour, keep the 10min enzyme denaturing, filter, collect filtrate, be evaporated to relative density and be 1.05 concentrated solution, in the time of 60 ℃; Wherein, described compound enzyme is the mixture of cellulase and pectase, and quality proportioning both is 1:1;
(2) flocculation remove impurity: under 60 ℃ of conditions, first the ratio in 80mL chitosan gum liquid solution/1000mL concentrated solution adds chitosan in concentrated solution, fully stir, ratio in 80mL 101 fruit juice clarifier aqueous solution/1000mL concentrated solutions adds 101 fruit juice clarifier aqueous solutions again, fully stir, hold over night, supernatant is got in centrifugalize; Wherein, described chitosan gum liquid solution is that volumetric concentration is that chitosan mass concentration that 1% acetum and chitosan are mixed is 1% acetum, and described 101 fruit juice clarifier aqueous solutions are that 101 fruit juice clarifier mass concentrations that water and 101 fruit juice clarifiers are mixed are 5% aqueous solution;
(3) cross macroporous resin column: supernatant is crossed the D101 resin column, is 50% ethanol elution with volumetric concentration, and eluent is concentrated, and drying under reduced pressure namely gets Radix Glycyrrhizae total flavones.
2. the method for extracting licoflavone from glycyrrhiza residue according to claim 1, it is characterized in that: the addition of the described composite enzyme solution of step (1) is 20ml composite enzyme solution/1g glycyrrhiza residue.
3. the method for extracting licoflavone from glycyrrhiza residue according to claim 2, it is characterized in that: the mass concentration of the described composite enzyme solution of step (1) is 1%.
4. the method for extracting licoflavone from glycyrrhiza residue according to claim 3, it is characterized in that: the pH value of the solution that the described glycyrrhiza residue of step (1) mixes with composite enzyme solution transfers to 5.0.
5. the method for extracting licoflavone from glycyrrhiza residue according to claim 4, it is characterized in that: the temperature of the described supersound process of step (1) is 50 ℃, and the time is 1 hour.
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