CN106880764A - A kind of preparation method of dendrobium candidum extract and its preparation - Google Patents
A kind of preparation method of dendrobium candidum extract and its preparation Download PDFInfo
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- CN106880764A CN106880764A CN201710142167.5A CN201710142167A CN106880764A CN 106880764 A CN106880764 A CN 106880764A CN 201710142167 A CN201710142167 A CN 201710142167A CN 106880764 A CN106880764 A CN 106880764A
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- dendrobium candidum
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/898—Orchidaceae (Orchid family)
- A61K36/8984—Dendrobium
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
Abstract
The present invention relates to technical field of plant extraction, more particularly to a kind of dendrobium candidum extract and its preparation preparation method.Organic solvent is not used in the dendrobium candidum extract preparation process that the present invention is provided, but uses zymolysis technique water extraction, by optimizing the species and consumption of enzyme, reach the purpose for improving yield, improving purity.Prepared molecular weight of product is smaller, and solubility property is preferable.Experiment shows that the yield that the method provided by the present invention extracts dendrobium candidum is 13.3%~18.8%；Wherein, the mass fraction of Dendrobium officinale polysaccharide is 74.9%~78.1%, and the mass fraction of polypeptide is 3.4%~4.2%.
Description
Technical field
The present invention relates to technical field of plant extraction, more particularly to a kind of dendrobium candidum extract and its preparation preparation side
Method.
Background technology
Dendrobium candidum (Classification system：Dendrobium officinale Kimuraet Migo), it is the rare medium-height grass of China
Medicine, is that orchid family Dendrobium Sw dries stem, strong with enriching yin of promoting the production of body fluid, improving eyesight nourishing the stomach, kidney tonifying because epidermis is gained the name in iron green
The effects such as body.According to the literature, the main pharmacodynamics composition in Dendrobium Sw is polysaccharide, alkaloid, glucosides, luxuriant and rich with fragrance class, bibenzyl
With amino acid etc..Content of the polysaccharide in dendrobium candidum is high, and makees with good antitumor, anti-oxidant and strengthen immunity etc.
With.The fresh stem of dendrobium candidum is direct-edible, or stews, or makes tea, or infusing drugs in wine, is generally processed into dry product, such as Tiepi Fengdou, iron
Skin stem of noble dendrobium functional beverage etc..The high-class healthy product for being produced as raw material with dendrobium candidum in recent years are more and more, be in great demand it is American-European and
The coastal developed regions in Southeast Asia.
Existing dendrobium candidum extracting method mostly using organic solvent as extract solution (for example：Methyl alcohol, ethanol, acetone,
Ethyl acetate etc.), dendrobium candidum is carried out using organic solvent extract low for equipment requirements, easily operation, low cost.But product
The content of middle active ingredient is relatively low, and molecular weight is larger and organic solvent residual reduces the security of product.Also, it is existing
Product be mainly ovendry power flour, water-soluble poor, product form is bad, have impact on absorption of the human body to active material.
The content of the invention
In view of this, the technical problem to be solved in the present invention is the system for providing a kind of dendrobium candidum extract and its preparation
Preparation Method, polyoses content enriches in dendrobium candidum extract obtained in the preparation method that the present invention is provided, and yield is higher.It is obtained
Extract is prevented from caking, and form is preferable.
The preparation method of the dendrobium candidum extract that the present invention is provided, including：
Step 1：Dendrobium candidum mixes with the water of 4 times~12 times of quality, cross colloid mill after, be heated to 95 DEG C it is maintained above
20min~60min, is then cooled to 50 DEG C~60 DEG C, and slurries are obtained；
Step 2：The slurries successively through plant hydrolyzed-enzyme and hydrolysis by novo after, the heated enzyme that goes out, cool down it is laggard
Row ultrafiltration, takes filtrate；
Step 3：After mixing with microcrystalline cellulose, beta-schardinger dextrin after the concentrating filter liquor, 40 DEG C of holdings are heated to
30min, is then spray-dried, and dendrobium candidum extract is obtained；
The plant hydrolyzed-enzyme is (0.1~2.0) with the mass ratio of dendrobium candidum：100；
The condition of the plant hydrolyzed enzyme hydrolysis is：PH value 3.0~4.0；55 DEG C of 50~150min of enzymolysis；
The alkali protease is (0.1~2.0) with the mass ratio of dendrobium candidum：100；
The condition of the hydrolysis by novo is：PH value 8.0~8.5；50 DEG C of 50~150min of enzymolysis；
The molecular weight of the ultrafiltration is 10kDa；
It is described that to be concentrated into total solid content in concentrate be 25%~40%；
The microcrystalline cellulose is (0.2~1.0) with the mass ratio of the concentrate：100；
The beta-schardinger dextrin is (0.4~0.8) with the mass ratio of the concentrate：100.
The present invention uses dendrobium candidum and dries stem (or fresh stem) and extracted, through cleaning, crushing before extracting.Crush
It is 80~120 mesh to granularity.
In some embodiments, dendrobium candidum mixes with the water of 4 times of quality, crosses colloid mill.
In other embodiments, dendrobium candidum mixes with the water of 12 times of quality, crosses colloid mill.
In other embodiments, dendrobium candidum mixes with the water of 10 times of quality, crosses colloid mill.
In the embodiment of the present invention, the power of the colloid mill is 7.5KW, and rotating speed is 2900R/min, and emulsification fineness is 2 μm.
After crossing colloid mill, 95 DEG C~100 DEG C are heated to.In some embodiments, 50 DEG C are cooled to after keeping 60min；It is another
In a little embodiments, 60 DEG C are cooled to after keeping 40min；In other embodiments, 55 DEG C are cooled to after keeping 20min.
By after colloid mill, dendrobium candidum is crushed in slurries, preferably to be digested.
Plant hydrolyzed-enzyme is the mixture of various enzymes, contains very strong pectin lyase and various carbohydrases, bag
Include arabanase, cellulase, dextranase, hemicellulase and zytase etc..The enzyme is also to branched chain type pectin class
The cellulose found such as in soya cells wall like thing is active, and conventional composite plant hydrolase is through the aspergillus of seed selection
(Aspergillus aculeatus) bacterial strain is produced.The enzyme activity of the plant hydrolyzed-enzyme that the present invention is used is 1,400,000 u/g.
In some embodiments, the plant hydrolyzed-enzyme is 0.1 with the mass ratio of dendrobium candidum：100；
The condition of the plant hydrolyzed enzyme hydrolysis is：PH value 3.0；55 DEG C of enzymolysis 50min；
The alkali protease is 0.1 with the mass ratio of dendrobium candidum：100；
The condition of the hydrolysis by novo is：PH value 8.0；50 DEG C of enzymolysis 50min.
In other embodiments, the plant hydrolyzed-enzyme is 2 with the mass ratio of dendrobium candidum：100；
The condition of the plant hydrolyzed enzyme hydrolysis is：PH value 4.0；55 DEG C of enzymolysis 150min；
The alkali protease is 2 with the mass ratio of dendrobium candidum：100；
The condition of the hydrolysis by novo is：PH value 8.5；50 DEG C of enzymolysis 150min.
In other embodiments, the plant hydrolyzed-enzyme is 0.5 with the mass ratio of dendrobium candidum：100；
The condition of the plant hydrolyzed enzyme hydrolysis is：PH value 3.5；55 DEG C of enzymolysis 90min；
The alkali protease is 0.5 with the mass ratio of dendrobium candidum：100；
The condition of the hydrolysis by novo is：PH value 8.5；50 DEG C of enzymolysis 90min.
The temperature of the enzyme that goes out is more than 95 DEG C 5min~15min；
In some embodiments, the temperature of the enzyme that goes out is 95 DEG C~100 DEG C, and the time is 5min.
In other embodiments, the temperature of the enzyme that goes out is 95 DEG C~100 DEG C, and the time is 15min.
In other embodiments, the temperature of the enzyme that goes out is 95 DEG C~100 DEG C, and the time is 10min.
Using vacuum concentration, it is 25%~40% to be concentrated into solid content for the concentration.
In some embodiments, it is 30% to be concentrated into solid content；
In other embodiments, it is 40% to be concentrated into solid content.
In other embodiments, it is 35% to be concentrated into solid content.
Addition microcrystalline cellulose is to prevent powder agglomeration, and addition cyclodextrin is to embed, it is easy to spray drying.
In some embodiments, the microcrystalline cellulose is 0.2 with the mass ratio of the concentrate：100；
The beta-schardinger dextrin is 0.4 with the mass ratio of the concentrate：100.
In some embodiments, the microcrystalline cellulose is 1 with the mass ratio of the concentrate：100；
The beta-schardinger dextrin is 0.8 with the mass ratio of the concentrate：100.
In some embodiments, the microcrystalline cellulose is 0.5 with the mass ratio of the concentrate：100；
The beta-schardinger dextrin is 0.5 with the mass ratio of the concentrate：100.
The steam pressure of spray drying of the present invention is 0.8MPa；EAT is 180 DEG C；Temperature of outgoing air is 90 DEG C；
Negative pressure is 200KPa in tower.
The yield of spray drying gained dendrobium officinale powder is 13.3%~18.8%；Wherein, the mass fraction of Dendrobium officinale polysaccharide
It is 74.9%~78.1%, the mass fraction of polypeptide is 3.4%~4.2%.Molecular weight is less than 10kDa；Do not contain organic molten
The residual of agent, and proterties is good after spray drying, easily dissolving.
Dendrobium candidum extract obtained in preparation method of the present invention.
Application of the dendrobium candidum extract obtained in preparation method of the present invention in the product for preparing regulation blood sugar.
In the present invention, the dosage of the dendrobium candidum extract regulation blood sugar is 0.05~0.6g/kg/d.
Experiment shows that the dendrobium candidum extract that the present invention is provided can have the effect for reducing blood sugar.
Present invention also offers a kind of product for adjusting blood sugar, it includes iron sheet stone obtained in preparation method of the present invention
Dry measure used in former times extract and auxiliary material.
In some embodiments, its formulation be tablet, capsule, pill, granule, decoction, paste, distillate medicinal water, oral solutions,
Pill, syrup.
In some specific embodiments, it is tablet, and its auxiliary material is citric acid, Sucralose, lactose, polyethylene glycol, tristearin
Sour magnesium and silica.
Specifically, in granule, the mass parts of each component are：
In some specific embodiments, it is granule, and its auxiliary material is citric acid and Sucralose.
Specifically, in tablet, the mass parts of each component are：
20.0 parts of dendrobium candidum extract；
1.0 parts of citric acid；
59.0 parts of white granulated sugar.
Organic solvent is not used in the dendrobium candidum extract preparation process that the present invention is provided, but uses zymolysis technique water
Carry, by optimizing the species and consumption of enzyme, reach the purpose for improving yield, improving purity.Prepared molecular weight of product is smaller, dissolving
Better performances.Experiment shows that the yield that the method provided by the present invention extracts dendrobium candidum is 13.3%~18.8%；Wherein, iron sheet
The mass fraction of dendrobium polysaccharide is 74.9%~78.1%, and the mass fraction of polypeptide is 3.4%~4.2%.
Specific embodiment
The invention provides a kind of dendrobium candidum extract and its preparation method of preparation, those skilled in the art can borrow
Mirror present disclosure, is suitably modified technological parameter realization.In particular, all similar replacements and change are to this area
It is it will be apparent that they are considered as being included in the present invention for technical staff.The method of the present invention and application have passed through
Preferred embodiment is described, and related personnel can substantially not depart from the side in present invention, spirit and scope to this paper
Method and application are modified or suitably change is realized and apply the technology of the present invention with combining.
The examination material that the present invention is used is all common commercially available product, can all be bought in market.
With reference to embodiment, the present invention is expanded on further：
Embodiment 1
Dendrobium candidum 10kg is taken, is cleaned, crushed, add 4 times of water of amount, cross twice colloid mill (parameter:Power is 7.5KW,
Rotating speed is 2900R/min, and emulsification fineness is 2 μm.), it is standby, it is heated to 95 DEG C 20 minutes, 50 DEG C are cooled to, slurries 36kg is obtained,
It is standby；
Slurries are taken, pH value to 3.0 is adjusted with 20% acid solution, add the plant hydrolyzed-enzyme of dendrobium candidum weight 10g, 55
DEG C enzymolysis 50min；PH value to 8.0 is adjusted with 20% aqueous slkali, the alkali protease of dendrobium candidum weight 10g, 50 DEG C is added
50min is digested, it is standby.
Being heated to 95 DEG C of 5min carries out enzyme-deactivating, is cooled to room temperature, standby.Take enzymolysis liquid to be filtered, obtain filtrate 31kg,
Filtrate crosses the milipore filter of 10k dalton, obtains filtered solution 28kg, standby.
Filtered solution is taken, solid content is concentrated in vacuo to for 30%, concentrate 5.2kg is obtained, it is standby.
Embodiment 2
Dendrobium candidum 10kg is taken, is cleaned, crushed, add 12 times of water of amount, cross twice colloid mill (parameter：Power is
7.5KW, rotating speed is 2900R/min, and emulsification fineness is 2 μm.), it is standby.It is heated to more than 95 DEG C 60 minutes, is cooled to 60 DEG C, obtains
Slurries 106kg, it is standby；
Slurries are taken, pH value to 4.0 are adjusted with 20% acid solution, add 200g plant hydrolyzed-enzymes, 55 DEG C digest 150min,
PH value to 8.5 is adjusted with 20% aqueous slkali, the alkali protease of 200g is added, 50 DEG C digest 150min, standby.
Being heated to 95 DEG C of 15min carries out enzyme-deactivating, is cooled to room temperature, standby.
Take enzymolysis liquid to be filtered, obtain filtrate 98kg, filtrate crosses the milipore filter of 10k dalton, obtain filtered solution 92kg, it is standby
With.
Take filtered solution and be concentrated into solid content for 40%, obtain concentrate 5.4kg, it is standby.
Embodiment 3
Dendrobium candidum 10kg is taken, is cleaned, crushed, add 10 times of water of amount, cross twice colloid mill (parameter：Power is
7.5KW, rotating speed is 2900R/min, and emulsification fineness is 2 μm.), it is standby；It is heated to more than 95 DEG C 40 minutes, is cooled to 55 DEG C, obtains
Slurries 96kg, it is standby；
Slurries are taken, pH value to 3.5 are adjusted with 20% acid solution, add the plant hydrolyzed-enzyme of 50g, 55 DEG C digest 90min,
It is standby；PH value to 8.0 is adjusted with 20% aqueous slkali, 50g alkali proteases are added, 50 DEG C digest 90min, standby.
Being heated to more than 95 DEG C 10min carries out enzyme-deactivating, is cooled to room temperature, standby.
Take enzymolysis liquid to be filtered, obtain filtrate 90kg, filtrate crosses the milipore filter of 10k dalton, obtain filtered solution 84kg, it is standby
With.
Filtered solution is taken, solid content is concentrated into for 35%, concentrate 6.2kg is obtained, it is standby；
Embodiment 4
The concentrate of Example 1, adds the microcrystalline cellulose of 10.4g and the beta-schardinger dextrin of 20.8g, is heated to 40 degree of water-baths
30 minutes, it is spray-dried (parameter：Steam pressure is 0.8MPa；EAT is 180 DEG C；Temperature of outgoing air is 90 DEG C；Negative pressure in tower
It is 200KPa.), obtain dendrobium candidum extract 1.33kg；
Embodiment 5
The concentrate of Example 2, adds 540g microcrystalline cellulose 43.2g beta-schardinger dextrins, is heated to 40 degree of water-baths 30 minutes,
Spray drying (parameter：Steam pressure is 0.8MPa；EAT is 180 DEG C；Temperature of outgoing air is 90 DEG C；Negative pressure is in tower
200KPa.), obtain dendrobium candidum extract 1.88kg.
Embodiment 6
The concentrate of Example 3, adds the microcrystalline cellulose of 31g and the beta-schardinger dextrin of 31g, is heated to 40 degree of 30 points of water-baths
Clock, is spray-dried (parameter：Steam pressure is 0.8MPa；EAT is 180 DEG C；Temperature of outgoing air is 90 DEG C；Negative pressure is in tower
200KPa.), obtain dendrobium candidum extract 1.86kg.
Comparative example 1
The concentrate of Example 2, adds the microcrystalline cellulose of 540g, is heated to 40 degree of water-baths 30 minutes, spray drying (ginseng
Number：Steam pressure is 0.8MPa；EAT is 150 DEG C；Temperature of outgoing air is 75 DEG C；Negative pressure is 200KPa in tower.), obtain iron sheet
Herba Dendrobii extract 0.36kg.
Comparative example 2
The concentrate of Example 2, adds 43.2g beta-schardinger dextrins, is heated to 40 degree of water-baths 30 minutes, is spray-dried (parameter：
Steam pressure is 0.8MPa；EAT is 200 DEG C；Temperature of outgoing air is 110 DEG C；Negative pressure is 200KPa in tower.), obtain iron sheet stone
Dry measure used in former times extract 0.58kg.
Comparative example 3
The concentrate of Example 2,540g microcrystalline cellulose 43.2g beta-schardinger dextrins are heated to 40 degree of water-baths 30 minutes, spraying
Dry (parameter：Steam pressure is 0.8MPa；EAT is 150 DEG C；Temperature of outgoing air is 75 DEG C；Negative pressure is 200KPa in tower.),
Obtain dendrobium candidum extract 0.89kg.
Embodiment 7
Example 4 is obtained dendrobium candidum extract 20.0g, adds 1.0g citric acids, 59.0g white granulated sugars and
10.0g75% ethanol carries out wet granulation, drying, is distributed into 8g/ bags of granule.
Embodiment 8
Example 4 is obtained dendrobium candidum extract 20.0g, adds 1.0 citric acids, 30.0 white granulated sugars, 20.0 lactose,
0.5 polyethylene glycol and 10.0g75% ethanol carry out wet granulation, drying, add 0.2g magnesium stearates and 0.3g silica, pressure
Piece machine carries out compressing tablet, obtains dendrobium candidum pressed candy.
Embodiment 9
Example 5 is obtained dendrobium candidum extract 20.0g, adds 1.0g citric acids, 59.0g white granulated sugars and
10.0g75% ethanol carries out wet granulation, drying, is distributed into 8g/ bags of granule.
Embodiment 10
Example 5 is obtained dendrobium candidum extract 20.0g, adds 1.0 citric acids, 30.0 white granulated sugars, 20.0 lactose,
0.5 polyethylene glycol and 10.0g75% ethanol carry out wet granulation, drying, add 0.2g magnesium stearates and 0.3g silica, pressure
Piece machine carries out compressing tablet, obtains dendrobium candidum pressed candy.
Embodiment 11
Example 5 is obtained dendrobium candidum extract 20.0g, adds 1.0g citric acids, 59.0g white granulated sugars and
10.0g75% ethanol carries out wet granulation, drying, is distributed into 8g/ bags of granule.
Embodiment 12
Example 6 is obtained dendrobium candidum extract 20.0g, adds 1.0 citric acids, 30.0 white granulated sugars, 20.0 lactose,
0.5 polyethylene glycol and 10.0g75% ethanol carry out wet granulation, drying, add 0.2g magnesium stearates and 0.3g silica, pressure
Piece machine carries out compressing tablet, obtains dendrobium candidum pressed candy.
Embodiment 13：Dendrobium candidum extract polysaccharide and protein content are detected：
First, polysaccharide detection method (anthrone-sulfuric acid process)：
(1) experimental principle：
Polysaccharide is first hydrolyzed into monose under effect of sulfuric acid, and is dehydrated generation alditol derivative, is generated with phenol orange-yellow molten
Liquid, there is absorption maximum at 490nm, and absorption value is linear with sugared content.
(2) reagent：
The concentrated sulfuric acid (analysis is pure), 5% phenol solution (analysis is pure), standard glucose.
(3) operating procedure：
1. standard curve is made：It is accurate to weigh 105 DEG C of drying and be made into 500ml volumetric flasks to the glucose 50mg of constant weight
The standard liquid of 0.1mg/ml, be made into 0 respectively with distilled water, 0.02,0.04,0.06,0.08,0.1mg/ml.1ml titers point
5% phenol solution 1ml is not added, and is rapidly added concentrated sulfuric acid 5ml, stand 10min.Shake up, 30 DEG C place 30min after
490nm determines OD values, and with glucose content as abscissa, OD values are ordinate, and making obtains standard curve.
2. sample size is determined：1.0ml sample liquids are drawn, according to above-mentioned steps time-and-motion study OD490, and it is bent to substitute into standard
The total sugar content of line computation sample.
2nd, polypeptide detection methods (with reference to GB T 22492-2008)
3rd, solubility detection (with reference to GB 5413.29-2010)
(4) testing result
Product checking result see the table below：
Table 1：Product checking result
| Polyoses content (%) | Content of peptides (%) | Solubility (%) | |
| Embodiment 4 | 75.3 | 3.4 | 98.2 |
| Embodiment 5 | 78.1 | 3.6 | 99.0 |
| Embodiment 6 | 74.9 | 4.2 | 98.7 |
| Comparative example 1 | 54.6 | 2.8 | 78.6 |
| Comparative example 2 | 50.5 | 2.6 | 78.5 |
| Comparative example 3 | 51.0 | 2.5 | 79.0 |
Polyoses content in the extract of comparative example 1~3 is lower than embodiment 1~3 by 20% or so, and comparative example 1~3 is obtained and carries
The solubility for taking thing is lower than embodiment by 20% or so, through statistical analysis, between embodiment 1~3 and comparative example 1~3,
In polyoses content, content of peptides, solubility level, all there is the difference (p of conspicuousness<0.05).Show the method provided by the present invention
The extract yield of preparation is higher, and polyoses content, content of peptides are all higher.Illustrate in extraction process, nutriment does not meet with
To destruction.Products therefrom solubility is good, easily facilitates the edible of people.
The dendrobium candidum extract efficacy validation of embodiment 14
Dendrobium candidum adjusts blood glucose test result
1 sample：Using the dendrobium candidum water extraction powder sample prepared by the various embodiments described above 4~6 and degree ratio 1~3；
2 experimental animals：Kunming mouse, is provided, 18.0~22.0g of body weight by unming Medical College's experimental animal center.
3 test methods and result：
Take Kunming mouse some, be randomly divided into 6 groups, such as table 1.Respectively blank group, positive drug group (Glibenclamide Tablets),
3 groups of model group, 1 group of embodiment, 2 groups of embodiment and embodiment.
After the preceding fasting of experiment 12 hours, the alloxan of tail vein injection 70mg/kg.The Portugal of tail vein injection 5% after 4 hours
Grape sugar 0.4ml.Experiment surveys blood glucose value when treating that blood sugar rises to peak period within the 3rd day, selects that blood glucose value ＞ 16.6mmol/l's is small
Mouse is as administration object.Each group gavage gives corresponding extract, and once a day, positive drug is sooner or later each once, successive administration 7
My god.Last dose surveys the blood glucose value of each group experiment mice after 2 hours.
The regulation blood sugar effect of the dendrobium candidum of table 2
| Group | Number of mice (only) | Dosage (g/kg) | Blood sugar average value (mmol/l) |
| Normal group | 10 | -- | 8.23 |
| Model group | 10 | -- | 22.19 |
| Embodiment 4 | 10 | 0.1 | 8.34 |
| Embodiment 5 | 10 | 0.1 | 8.37 |
| Embodiment 6 | 10 | 0.1 | 8.25 |
| Comparative example 1 | 10 | 0.1 | 9.20 |
| Comparative example 2 | 10 | 0.1 | 9.33 |
| Comparative example 3 | 10 | 0.1 | 9.56 |
| Positive control | 10 | 0.1 | 8.02 |
Model and blank relatively have significant difference (P ＜ 0.01), the modeling success of this time experiment.And give each embodiment
There is the difference (p of conspicuousness with the blood sugar of the model for giving comparative example extract in the blood sugar of the model of extract<0.05).And it is each
It is similar to the effect of positive drug glibenclamide that embodiment is obtained effect of the extract in terms of blood sugar is adjusted.
The above is only the preferred embodiment of the present invention, it is noted that come for those skilled in the art
Say, under the premise without departing from the principles of the invention, can also make some improvements and modifications, these improvements and modifications also should be regarded as
Protection scope of the present invention.
Claims (10)
1. a kind of preparation method of dendrobium candidum extract, it is characterised in that including：
Step 1：Dendrobium candidum mixes with the water of 4 times~12 times of quality, after crossing colloid mill, is heated to 95 DEG C of 20min maintained above
~60min, is then cooled to 50 DEG C~60 DEG C, and slurries are obtained；
Step 2：The slurries successively through plant hydrolyzed-enzyme and hydrolysis by novo after, the heated enzyme that goes out, cooling after surpassed
Filter, takes filtrate；
Step 3：After mixing with microcrystalline cellulose, beta-schardinger dextrin after the concentrating filter liquor, 40 DEG C of holding 30min are heated to, so
After be spray-dried, dendrobium candidum extract is obtained；
The plant hydrolyzed-enzyme is (0.1~2.0) with the mass ratio of dendrobium candidum：100；
The condition of the plant hydrolyzed enzyme hydrolysis is：PH value 3.0~4.0；55 DEG C of 50~150min of enzymolysis；
The alkali protease is (0.1~2.0) with the mass ratio of dendrobium candidum：100；
The condition of the hydrolysis by novo is：PH value 8.0~8.5；50 DEG C of 50~150min of enzymolysis；
The molecular weight of the ultrafiltration is 10kDa；
It is described that to be concentrated into total solid content in concentrate be 25%~40%；
The microcrystalline cellulose is (0.2~1.0) with the mass ratio of the concentrate：100；
The beta-schardinger dextrin is (0.4~0.8) with the mass ratio of the concentrate：100.
2. preparation method according to claim 1, it is characterised in that the power of the colloid mill is 7.5KW, rotating speed is
2900R/min, emulsification fineness is 2 μm.
3. preparation method according to claim 1, it is characterised in that the steam pressure of the spray drying is 0.8MPa；
EAT is 180 DEG C；Temperature of outgoing air is 90 DEG C；Negative pressure is 200KPa in tower.
4. dendrobium candidum extract obtained in preparation method described in any one of claims 1 to 3.
5. dendrobium candidum extract obtained in preparation method described in any one of claims 1 to 3 is preparing the product of regulation blood sugar
In application.
6. application according to claim 5, it is characterised in that the dosage of dendrobium candidum extract regulation blood sugar is
0.05~0.6g/kg/d.
7. it is a kind of adjust blood sugar product, it is characterised in that including obtained in preparation method described in any one of claims 1 to 3
Dendrobium candidum extract and auxiliary material.
8. product according to claim 7, it is characterised in that its formulation is tablet, capsule, pill, granule, soup
Agent, paste, distillate medicinal water, oral solutions, pill, syrup.
9. product according to claim 7, it is characterised in that it is tablet, its auxiliary material is citric acid, Sucralose, breast
Sugar, polyethylene glycol, magnesium stearate and silica.
10. product according to claim 7, it is characterised in that it is granule, its auxiliary material is citric acid and trichlorine sugarcane
Sugar.
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| CN107446978A (en) * | 2017-08-15 | 2017-12-08 | 陈玫辛 | A kind of stem of noble dendrobium small-molecular peptides and preparation method thereof |
| CN112592793A (en) * | 2021-01-05 | 2021-04-02 | 西南林业大学 | Dendrobium officinale extract fermented wine and preparation method thereof |
| CN114344187A (en) * | 2022-01-10 | 2022-04-15 | 南宁荣港生物科技有限公司 | Chrysanthemum megatherum polypeptide composition and preparation method and application thereof |
| CN114632021A (en) * | 2022-05-20 | 2022-06-17 | 广州优理氏生物科技有限公司 | Polypeptide enzymolysis preparation, preparation method thereof and application of polypeptide enzymolysis preparation in skin care products |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107446978A (en) * | 2017-08-15 | 2017-12-08 | 陈玫辛 | A kind of stem of noble dendrobium small-molecular peptides and preparation method thereof |
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| CN114344187A (en) * | 2022-01-10 | 2022-04-15 | 南宁荣港生物科技有限公司 | Chrysanthemum megatherum polypeptide composition and preparation method and application thereof |
| CN114632021A (en) * | 2022-05-20 | 2022-06-17 | 广州优理氏生物科技有限公司 | Polypeptide enzymolysis preparation, preparation method thereof and application of polypeptide enzymolysis preparation in skin care products |
| CN114632021B (en) * | 2022-05-20 | 2022-08-02 | 广州优理氏生物科技有限公司 | Polypeptide enzymolysis preparation, preparation method thereof and application of polypeptide enzymolysis preparation in skin care products |
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