CN109329022A - A kind of selection of accumulation type high-speed rail, low lead water rice varieties - Google Patents

A kind of selection of accumulation type high-speed rail, low lead water rice varieties Download PDF

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Publication number
CN109329022A
CN109329022A CN201811231053.9A CN201811231053A CN109329022A CN 109329022 A CN109329022 A CN 109329022A CN 201811231053 A CN201811231053 A CN 201811231053A CN 109329022 A CN109329022 A CN 109329022A
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speed rail
lead
generation
accumulation type
parent
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梅淑芳
张树生
朱友银
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Jinhua Polytechnic
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Jinhua Polytechnic
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Priority to CN202110359303.2A priority patent/CN113057094A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00Other inorganic fertilisers
    • C05D9/02Other inorganic fertilisers containing trace elements
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Environmental Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Genetics & Genomics (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Inorganic Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention discloses a kind of accumulation type high-speed rails, the selection of low lead water rice varieties, comprising: selecting high Fe content rice to accumulate rice for parent A, low heavy metal is parent B, and parent A is hybridized with parent B, obtains F1For seed;Plant single cross F1In generation, is returned with parent A, obtains BC1F1For seed;BC1F1In generation, harvests seed by single plant using Anther Culture building plus DH group;DH1Generation, the method for taking various concentration high iron solution water planting select the strain of accumulation type high-speed rail;DH2Generation, the method for taking lead at different concentrations solution water planting select the high-speed rail strain of the low lead of accumulation type;DH3In generation, continuously plants in slight lead contamination area crop field, and low lead, high-speed rail strain are stablized in selection;DH4In generation, selects low lead, high-speed rail strain in the common field planting of unleaded contaminated areas;The present invention is the beneficial combination beneficial to micro- nutrition high-speed rail and high zinc content and low heavy metal accumulation, and mouthfeel more preferably the advantages of.

Description

A kind of selection of accumulation type high-speed rail, low lead water rice varieties
Technical field
The present invention relates to the breedings of rice breeding technology field more particularly to a kind of accumulation type high-speed rail, low lead water rice varieties Method.
Background technique
Ferro element is one of the essential element for constituting human body, is the constituent of hemoglobin, hemoglobin ginseng With the transport and storage of oxygen.Iron deficiency influences whether the health and development of human body, and maximum influence is hypoferric anemia.According to the world Health organization data show there is nearly 3,700,000,000 population iron deficiency in the world, wherein 2,000,000,000 people suffer from iron-deficiency anemia.Woman is not given birth to Have 40% in female, and there is 50% people to suffer from hypoferric anemia in pregnant woman.Therefore, carry out high-speed rail rice breeding, implement diet plan Slightly meet the needs of melt quality from source, is of great significance to the status for mitigating and improving asiderosis.
In recent years, the successive generation of " poison rice " event, the focus that rice quality becomes as public concern.Lead contamination is to youngster Virgin harm is serious, and nervous system, blood forming organ and kidney etc. can be caused significantly to change, blood lead height and children's intelligence (IQ) Inversely.There are problems that more serious lead contamination in Jinhua Yongkang hardware and its periphery.Therefore, reinforce high-speed rail to contain Amount, low lead accumulate new rice variety breeding, are more advantageous to the industry development for pushing local auxotype Safe Agricultural Product.
Summary of the invention
The purpose of the present invention is to provide a kind of accumulation type high-speed rails, the selection of low lead water rice varieties, can be vast While pregnant woman provides high-speed rail Nutritive Rice, the safer low lead rice trusted is also provided.
A kind of selection of accumulation type high-speed rail, low lead water rice varieties, comprising:
(1) selecting high-speed rail rice to accumulate rice for parent A, low lead is parent B, and parent A is hybridized with parent B, obtains F1 For seed;
(2) single cross F is planted1In generation, is returned with parent A, obtains BC1F1For seed;
(3) BC is planted1F1In generation, DH group is constructed using Anther Culture, harvests seed by single plant;
(4) DH is planted by strain1In generation, selects the strain of accumulation type high-speed rail with the method for various concentration ferrous solution water planting;
(5) DH is planted2In generation, selects the high-speed rail strain of the low lead of accumulation type with the method for lead at different concentrations solution water planting;
(6) DH is planted3Generation, in lead slight pollution area field planting, the high-speed rail strain of the low lead of selection accumulation type;
(7) DH is planted4In generation, selects the high-speed rail strain of low lead content in the common field planting of unleaded contaminated areas;
(8) DH is planted5Generation, select accumulation type high Fe content, low lead content sizing rice to get to accumulation type high-speed rail, Low lead water rice varieties.
Further, the parent A is that purple blood is glutinous.
Glutinous purple blood is the local precious local varieties relatively planted such as Guizhou, Yunnan, belongs to purple glutinous rice, color purple is black, food flavor It is fragrant and sweet, it is agreeably sweet, it can facilitate and be obtained from China Paddy Rice Inst's Rice Germplasm Resources center.Iron in the rice varieties brown rice Content is up to 36.2mg/kg.
Further, the parent B is Xiushui 128.
The Fine Quality Rice Variety of Xiushui 128 Jiaxing City, Zhejiang Province academy of agricultural science breeding in 2015 (number: examine by Shanghai Rice 2015005).The kind system national standard level-one superior rice, low lead are accumulated, and as common rice, content is iron content in brown rice 19.2mg/kg。
Select glutinous purple blood, Xiushui 128 hybridization combo and with the glutinous backcrossing of purple blood, the rice of breeding high-speed rail, low lead beneficial combination, Meet the growth requirement of local safe and high quality.
It further, is FeSO used in the method for the high iron solution water planting in step (4)4The salting liquid of preparation, Concentration of iron is 10 μm of ol/L, 20 μm of ol/L, 40 μm of ol/L.
It further, is PbSO used in the lead at different concentrations solution ciltivating process in step (5)4The salt of preparation Solution, the concentration of lead are respectively 0.5mg/kg, 1.0mg/kg, 2.0mg/kg.
Further, in step (6), the lead slight pollution area crop field, the concentration of lead is respectively 0.2~0.5mg/ kg。
Compared with prior art, the invention has the benefit that
(1) present invention uses the ciltivating process of various concentration, is conducive to identification selection accumulation type high-speed rail and low lead is specifically new Kind is conducive to the crop field popularizing planting in common and slight pollution.
(2) high-speed rail of breeding of the present invention, low lead beneficial combination rice, while providing high-speed rail Nutritive Rice for pregnant woman, Safe and secure low heavy metal (low lead, low cadmium and low arsenic) rice is provided.
(3) present invention is remarkably improved the segregation ratio of selection offspring's high Fe content using the strategy being returned with high-speed rail parent Example, helps to realize the primary goal of high melt quality, lays the foundation for production nutrition+green agricultural product.
(4) present invention uses DH breeding technique, and required character is stable when using water planting selection, is more advantageous to screening Identification.
Specific embodiment
1, breeding material:
(1) it is parent A that purple blood is glutinous.Glutinous purple blood is the local precious local varieties relatively planted such as Guizhou, Yunnan, is belonged to purple glutinous Rice, color purple is black, and food flavor is fragrant and sweet, agreeably sweet, can facilitate and obtain from China Paddy Rice Inst's Rice Germplasm Resources center.The water Iron content in rice varieties brown rice is up to 36.2mg/kg, and lead accumulation characteristics and common rice are general.
(2) with Xiushui 128 for parent B.The rice of Xiushui 128 Jiaxing City, Zhejiang Province academy of agricultural science breeding in 2015 New varieties (number: rice 2015005 is examined in Shanghai).The kind system national standard level-one superior rice, the accumulation of low lead, iron content and common in brown rice Rice is similar, content 19.2mg/kg.
2, a kind of Breeding Process of high-speed rail rice are as follows:
In (1) 2012 year spring, Hainan Lingshui is glutinous for parent A with purple blood, makees female parent, miscellaneous with parent B (Xiushui 128) respectively It hands over, obtains F1(purple blood glutinous/Xiushui 128) is for 5, seed;
F is planted in (2) 2012 year summer and autumn, Jinhua, Zhejiang Yongkang1(purple blood glutinous/Xiushui 128) for 5 plants of plant, with parent A The purple glutinous backcrossing of blood obtains BC1F1(purple blood is glutinous // and purple blood is glutinous/Xiushui 128) generation 23;
(3) 2013 year spring, Hainan Lingshui, plantation backcrossing BC1F1(purple blood is glutinous // and purple blood is glutinous/Xiushui 128) 21 plants of generation, it adopts Doubled haploid (DH) group is constructed with Anther Culture, by single-strain planting and harvests DH0For seed, 67, DH system is constructed;
(4) 2013 year summer and autumn, Jinhua, Zhejiang, DH1In generation, takes FeSO4Preparation concentration of iron be 10 μm of ol/L, 20 μm of ol/L, The solution water planting of 40 μm of ol/L selects strain 31 of accumulation type high-speed rail;
(5) 2014 year summer and autumn, DH2Generation, using PbSO4Prepare 0.5mg/kg, 1.0mg/kg, 2.0mg/kg lead concentration Solution water planting selects strain 8 of the low lead of accumulation type, selects strain 6 of the high zinc content of low cadmium when mature by single plant measurement;
(6) 2015 year summer and autumn, summer and autumn, Jinhua, Zhejiang Yongkang, DH3Generation, slight lead heavy metal pollution area crop field (lead Concentration is 0.2~0.5mg/kg) plantation, select high-speed rail strain 5 of low lead content;
(7) 2016 year summer and autumn, Jinhua, Zhejiang, DH4In generation, selects low lead to contain in the common field planting of unleaded contaminated areas The high-speed rail strain of amount 3.
(8) 2017 year summer and autumn, Jinhua, Zhejiang, DH5In generation, selects 1, sizing rice that high Fe content, low lead are accumulated, i.e., Obtain accumulation type high-speed rail, low lead water rice varieties.
The main index of sizing rice of embodiment breeding: the efficient combination of high-speed rail, low lead, i.e., iron content reaches in brown rice 35.7mg/kg, in lead contamination field planting, lead accumulation is significant lower.And the iron content in parent's A brown rice is up to 36.2mg/ Kg, in lead contamination field planting also can with common rice is similar the problem of lead contamination occurs;Iron content is in parent's B brown rice 19.2mg/kg, similar with common rice, in lead contamination field planting, lead accumulation is significantly lower.
After above-mentioned screening obtains approved product, field planting is carried out in common rice, and to the mine of its multiple beneficial Matter nutrient and harmful contents of heavy metal elements are determined, and approved product and its parent's index determining are shown in Table 1.
Table 1, purple blood is glutinous, the beneficial mineral nutrition and harmful heavy metal content of Xiushui 128 and approved product compare
The measuring method of each index is as follows in table 1:
Calcium: GB/T5009.92-2003;
Iron: GB/T5009.90-2003;
Phosphorus: GB/T5009.87-2003;
Potassium: GB/T5009.91-2003;
Magnesium: GB/T5009.90-2003;
Zinc: GB/T5009.14-2003;
Mercury: GB/T5009.17-2003;
Arsenic: GB/T5009.11-2003;
Lead: GB/T5009.12-2003;
Cadmium: GB/T5009.15-2003.
On beneficial Mineral nutrients, calcium in approved product, potassium, phosphorus, magnesium equal size are similar with 2 parents', and iron contains Amount is 35.7mg/kg, is significantly higher than Xiushui 128, glutinous similar with purple blood, and Zn content is higher than 2 parents.On heavy metal element, Except the content and parent's Xiushui 128 of target lead are similar, mercury and cadmium are not detected, and arsenic content is more similar than parent or low.
Zinc is " flowers of life " by good reputation, has and improves intelligence, enhancing development, improves sense of taste promotion appetite, enhancing The multiple functions such as immunity of organisms.Approved product facilitates implementation diet strategy and meets the needs of zinc nutrition from source.
In addition, to approved product and two parents, in the relatively higher crop field of lead content, (lead content is between 0.2~0.4mg/ Kg the main rice quality index after) planting is detected (table 2), and discovery approved product growth is more normal, and the grain of rice is full, It is big to show significantly higher polished rice rate, exterior quality good (chalkness ratio is low, chalkiness degree is small), gel consistence, rice is soft and not glutinous, eats Mouthfeel is more preferable, and nutritive value is more preferable (protein content is high).
The purple blood of table 2 is glutinous, the main quality index and rice mouthfeel of Xiushui 128 and approved product compare
Therefore, this sizing rice is more advantageous to the health care of the mankind than parent product, especially can provide height for numerous pregnant woman While iron and high-zinc nutrient rice, the safer low heavy metal rice trusted is provided, taste is good to be easier to be received.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any The change or replacement expected without creative work should all be covered subject to this practical new protection scope.

Claims (6)

1. the selection of a kind of accumulation type high-speed rail, low lead water rice varieties, which comprises the following steps:
(1) selecting high-speed rail rice to accumulate rice for parent A, low lead is parent B, and parent A is hybridized with parent B, obtains F1Generation kind Son;
(2) single cross F is planted1In generation, is returned with parent A, obtains BC1F1For seed;
(3) BC is planted1F1In generation, DH group is constructed using Anther Culture, harvests seed by single plant;
(4) DH is planted by strain1In generation, selects the strain of accumulation type high-speed rail with the method for various concentration ferrous solution water planting;
(5) DH is planted2In generation, selects the high-speed rail strain of the low lead of accumulation type with the method for lead at different concentrations solution water planting;
(6) DH is planted3Generation, in lead slight pollution area field planting, the high-speed rail strain of the low lead of selection accumulation type;
(7) DH is planted4In generation, selects the high-speed rail strain of low lead content in the common field planting of unleaded contaminated areas;
(8) DH is planted5Generation, select accumulation type high Fe content, low lead content sizing rice to get arrive accumulation type high-speed rail, low lead water Rice varieties.
2. the selection of accumulation type high-speed rail according to claim 1, low lead water rice varieties, which is characterized in that described Parent A is that purple blood is glutinous.
3. the selection of accumulation type high-speed rail according to claim 1, low lead water rice varieties, which is characterized in that described Parent B is Xiushui 128.
4. the selection of accumulation type high-speed rail according to claim 1, low lead water rice varieties, which is characterized in that step (4) In, it is FeSO used in the various concentration ferrous solution ciltivating process4The salting liquid of preparation, concentration are 10 μm of ol/L, 20 μ mol/L、40μmol/L。
5. the selection of accumulation type high-speed rail according to claim 1, low lead water rice varieties, which is characterized in that step (5) In, it is PbSO used in the lead at different concentrations solution ciltivating process4The salting liquid of preparation, the concentration of lead are respectively 0.5mg/ kg、1.0mg/kg、2.0mg/kg。
6. the selection of accumulation type high-speed rail according to claim 1, low lead water rice varieties, which is characterized in that step (6) In, the lead slight pollution area crop field, the concentration of lead is 0.2~0.5mg/kg.
CN201811231053.9A 2018-10-22 2018-10-22 A kind of selection of accumulation type high-speed rail, low lead water rice varieties Pending CN109329022A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114946645A (en) * 2022-05-26 2022-08-30 广西星霞农业科技有限公司 Breeding method of high-iron low-cadmium rice variety

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108293872A (en) * 2018-03-29 2018-07-20 杭州中泽生物科技有限公司 A kind of selection of the low cadmium rice varieties of high zinc
CN108432629A (en) * 2018-02-12 2018-08-24 浙江大学 A kind of selection of the safe rice varieties of high-zinc nutrient
CN108476968A (en) * 2018-02-12 2018-09-04 浙江大学 A kind of selection of the low cadmium rice varieties of high ferro

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Publication number Priority date Publication date Assignee Title
CN106888963B (en) * 2017-03-08 2018-12-21 徐开盛 A kind of pregnant woman spy meals seed selection method for rice of high-resistance starch and the polymerization of high zinc character
CN106900543A (en) * 2017-03-29 2017-06-30 浙江大学 A kind of selection of the disease resisting rice kind of many nutrition polymerizations

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108432629A (en) * 2018-02-12 2018-08-24 浙江大学 A kind of selection of the safe rice varieties of high-zinc nutrient
CN108476968A (en) * 2018-02-12 2018-09-04 浙江大学 A kind of selection of the low cadmium rice varieties of high ferro
CN108293872A (en) * 2018-03-29 2018-07-20 杭州中泽生物科技有限公司 A kind of selection of the low cadmium rice varieties of high zinc

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114946645A (en) * 2022-05-26 2022-08-30 广西星霞农业科技有限公司 Breeding method of high-iron low-cadmium rice variety

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