CN109294743A - A kind of processing method improving agilawood essential oil yield - Google Patents
A kind of processing method improving agilawood essential oil yield Download PDFInfo
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- CN109294743A CN109294743A CN201811200667.0A CN201811200667A CN109294743A CN 109294743 A CN109294743 A CN 109294743A CN 201811200667 A CN201811200667 A CN 201811200667A CN 109294743 A CN109294743 A CN 109294743A
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- agalloch eaglewood
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
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- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/025—Recovery by solvent extraction
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
- C12N1/185—Saccharomyces isolates
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
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- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/85—Saccharomyces
- C12R2001/865—Saccharomyces cerevisiae
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- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
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Abstract
The invention discloses a kind of processing methods for improving agilawood essential oil yield, after including the following steps: that (1) pulverizes and sieves agalloch eaglewood, fresh agalloch eaglewood leaf, saccharomyces cerevisiae and water is added, is mixed evenly, controls the moisture content of mixed material between 60~90%；(2) said mixture material is placed in 25~32 DEG C of sealed environment, ferment 48h or more；(3) by the dry materials after fermentation, supercritical extract is then carried out, obtains agilawood essential oil.The yield of essential oil can be improved 30% or more by the present invention.Meanwhile the present invention is leavening using saccharomycete is commonly used in food industry, eaglewood leaf is auxiliary leavening, and technological operation is simple, has better economy and the feature of environmental protection.
Description
Technical field
The present invention relates to a kind of processing methods for improving agilawood essential oil yield.
Background technique
Agalloch eaglewood has very high status in numerous fragrance, is known as first of " heavy wingceltis dragon musk deer " four big name perfume.Be China,
Traditional rare medicinal herbs of Japan, India and middle east, is also used as high-grade fragrance always.Agilawood essential oil is agalloch eaglewood
In effective component, there is promoting qi circulation and relieving pain, warming middle energizer to arrest vomiting, gas of receiving to relieving asthma and other effects, had been widely used for after extracted medicine,
The fields such as cosmetics, food.Home-made occluder be Thymelaeceae eaglewood suspension culture of Aquilaria sinensis, be mainly grown in Hainan, Guangdong, Fujian,
The ground such as Yunnan, the agalloch eaglewood of different sources by its grow weather, the different agilawood essential oils born of microbiologic population in quality and
Also there is very big difference in quantity.
It is seldom that agalloch eaglewood is naturally occurred in nature, essential oil content is also very low, simultaneously because its huge use value, domestic
Outer scholar has conducted extensive research raising agilawood essential oil yield and its extraction efficiency.The suspension culture of Aquilaria sinensis plant of health does not generate heavy
Perfume (or spice) only (is chopped wound, burrows, connects bacterium, injection Edgeworthia chrysantha by natural cause (thunder split, burn, damage by worms, rotten etc.) or human factor
Liquid etc.) effect, tree body could wound and its around gradually form agilawood essential oil.About the mechanism that agalloch eaglewood is formed, mainstream
Viewpoint be considered to be invaded by fungi after trunk damage it is parasitic, in thallus under the action of enzyme, the starch of wood-parenchymatous cell's storage
It changes, through many years deposition to form face cream.Essential oil content is lower in agalloch eaglewood, is usually no more than 2%, in order to as far as possible
It is extracted, people have carried out a large amount of research.The method extracted currently used for agilawood essential oil mainly has steam distillation
Method and supercritical fluid extraction.Wherein, the recovery rate of steam distillation is lower, and easily causes heat during the extraction process not
Stablize the destruction and volatilization loss for leading to oxidizable ingredient.Supercritical CO2Fluid extraction technology in the environment of close to room temperature into
Row extraction, will not destroy bioactive ingredients, and have easy to operate, pollution-free, the features such as low energy consumption, and separating capacity is strong,
It is the common technology that current agilawood essential oil is largely extracted.Agilawood essential oil is distributed in the lignin of agalloch eaglewood, in order to which it is efficient
It extracts, needs to carry out agalloch eaglewood suitable pretreatment.Li Mingyue etc. [research of pectin enzyme pretreatment assisted extraction agilawood essential oil]
Deng using the effect of pectin in pectase enzyme hydrolysis agalloch eaglewood, getting through agilawood essential oil dissolution channel assisted extraction agilawood essential oil, make
The recovery rate of essential oil improves 11.05%.Bow is precious to wait and [investigates influence of the different fermentations time to agilawood essential oil recovery rate] investigation
Agalloch eaglewood is in the case where spontaneous fermentation, influence of the fermentation time to agilawood essential oil recovery rate, it is found that spontaneous fermentation institute for 24 hours
The yield of the agilawood essential oil obtained is higher.Li Xiang etc. [process optimization of supercritical carbon dioxide extracting agilawood essential oil] the study found that
Agalloch eaglewood is soaked in water processing, the extension within the regular hour with soaking time is conducive to improve agilawood essential oil
Extract yield.
Summary of the invention
The purpose of the present invention is complicated for operation, the higher costs, to essence for the existing pretreating process for extracting agilawood essential oil
Oily yield promotes limited disadvantage, provides a kind of extracting process for improving agilawood essential oil yield.By addition saccharomyces cerevisiae and newly
Fresh eaglewood leaf and agalloch eaglewood co-fermentation, increase substantially the yield of agilawood essential oil.
The present invention to achieve the above object, is achieved through the following technical solutions:
A kind of processing method improving agilawood essential oil yield, includes the following steps:
(1) after agalloch eaglewood pulverizing and sieving, fresh agalloch eaglewood leaf, saccharomyces cerevisiae and water is added, is mixed evenly, control
The moisture content of mixed material is between 60~90%；
(2) said mixture material is placed in 25~32 DEG C of sealed environment, ferment 48h or more；
(3) by the dry materials after fermentation, supercritical extract is then carried out, obtains agilawood essential oil.
Preferably, the additive amount of step (1) the agalloch eaglewood leaf is the 5%~25% of agalloch eaglewood quality.
Preferably, the additive amount of the agalloch eaglewood leaf is the 10%~20% of agalloch eaglewood quality.
Preferably, the additive amount of step (2) described saccharomyces cerevisiae is the 0.1%-0.01% of agalloch eaglewood quality.
Preferably, the additive amount of the saccharomyces cerevisiae is the 0.03%-0.06% of agalloch eaglewood quality.
Preferably, step (2) described supercritical extract is using dehydrated alcohol as entrainer.
Preferably, the condition of step (2) described supercritical extract are as follows: extracting pressure 20-30MPa, 40-50 DEG C of temperature, material
Liquor ratio 1:(1~2), 1~3h of extraction time；More preferable extracting pressure 28MPa, 46 DEG C of temperature, solid-liquid ratio 1:1.2, extraction time
2h。
Preferably, step (2) fermentation time is 48~60h.
Preferably, step (1) described agalloch eaglewood smashes it through 40-100 mesh.
Preferably, step (1) described agalloch eaglewood smashes it through 80 meshes.
It is existing the study found that using can with the enzyme preparation of depolymerized pectin, cellulose pre-process agalloch eaglewood timber, extraction can be improved
The yield of agilawood essential oil when taking.But due to enzyme preparation higher cost, treatment effeciency is very low when acting on agalloch eaglewood.Study table
It is bright, suitable stack retting is carried out to agalloch eaglewood and is handled, fermentation efficiency is high, and the yield of agilawood essential oil can be improved.But stack retting is handled
Technique is difficult to normalizing operation, so that product quality is difficult to stablize.The present invention is using commonly saccharomyces cerevisiae is in food industry
Leavening, fresh eaglewood leaf are that leavening is assisted to pre-process the woody part rich in agilawood essential oil, can significantly be mentioned
Yield when high agilawood essential oil extracts.
Compared with prior art, the beneficial effects of the present invention are:
(1) present invention carries out means of supercritical extraction after will be enriched in the woody part fermentation process of agilawood essential oil again, can will be smart
The yield of oil improves 30% or more.
(2) present invention is leavening using saccharomycete is commonly used in food industry, and eaglewood leaf is auxiliary leavening, technological operation
Simply, there is better economy and the feature of environmental protection.
Specific embodiment
Introduce implementation of the invention in more detail by the following examples.In the described embodiment, all percentages with
Quality meter.
Embodiment 1
The agalloch eaglewood 1000g (dry weight) of artificial Edgeworthia chrysantha is crushed, 40 meshes are crossed.In eaglewood powder after sizing, addition
3g saccharomyces cerevisiae dry powder, the fresh agalloch eaglewood leaf that 100g is crushed.In mixture, the water of 2000g is added, control moisture content exists
60% or more.Material stirring is uniformly placed in hermetic bag, is taken out after 48h is left to ferment in 30 DEG C of constant temperature incubation in room
It is dried under temperature.Fermentation mixture after drying carries out supercritical extract after crossing 40 meshes.Using dehydrated alcohol as entrainer, extraction
Pressure 28MPa, 46 DEG C of temperature, solid-liquid ratio 1:1.2, extraction time 2h.Agilawood essential oil extraction yield is 2.13% with this condition.
Embodiment 2
The agalloch eaglewood 1000g (dry weight) of artificial Edgeworthia chrysantha is crushed, 80 meshes are crossed.In eaglewood powder after sizing, addition
3g saccharomyces cerevisiae dry powder, the fresh agalloch eaglewood leaf that 100g is crushed.In mixture, the water of 2000g is added, control moisture content exists
60% or more.Material stirring is uniformly placed in hermetic bag, is taken out after 48h is left to ferment in 30 DEG C of constant temperature incubation in room
It is dried under temperature.Fermentation mixture after drying carries out supercritical extract after crossing 40 meshes.Using dehydrated alcohol as entrainer, extraction
Pressure 28MPa, 46 DEG C of temperature, solid-liquid ratio 1:1.2, extraction time 2h.Agilawood essential oil extraction yield is 2.21% with this condition.
Embodiment 3
The agalloch eaglewood 1000g (dry weight) of artificial Edgeworthia chrysantha is crushed, 40 meshes are crossed.In eaglewood powder after sizing, addition
6g saccharomyces cerevisiae dry powder, the fresh agalloch eaglewood leaf that 200g is crushed.In mixture, the water of 2500g is added, control moisture content exists
70% or more.Material stirring is uniformly placed in hermetic bag, is taken out after 48h is left to ferment in 30 DEG C of constant temperature incubation in room
It is dried under temperature.Fermentation mixture after drying carries out supercritical extract after crossing 40 meshes.Using dehydrated alcohol as entrainer, extraction
Pressure 28MPa, 46 DEG C of temperature, solid-liquid ratio 1:1.2, extraction time 2h.Agilawood essential oil extraction yield is 2.35% with this condition.
Comparative example 1
The agalloch eaglewood 1000g (dry weight) of artificial Edgeworthia chrysantha is crushed, carries out supercritical extract after crossing 40 meshes.With dehydrated alcohol
For entrainer, extracting pressure 28MPa, 46 DEG C of temperature, solid-liquid ratio 1:1.2, extraction time 2h.Agilawood essential oil extracts with this condition
Rate is 1.54%.
Comparative example 2
The agalloch eaglewood 1000g (dry weight) of artificial Edgeworthia chrysantha is crushed, 40 meshes are crossed.In eaglewood powder after sizing, addition
The fresh agalloch eaglewood leaf that 100g is crushed.In mixture, the water of 2000g is added, controls moisture content 60% or more.By material
It stirs evenly and is placed in hermetic bag, take out after standing for fermentation 48h in 30 DEG C of constant temperature incubation and dry at room temperature.It will dry
Fermentation mixture afterwards carries out supercritical extract after crossing 40 meshes.Using dehydrated alcohol as entrainer, extracting pressure 28MPa, temperature
46 DEG C, solid-liquid ratio 1:1.2, extraction time 2h.Agilawood essential oil extraction yield is 1.61% with this condition.
Comparative example 3
The agalloch eaglewood 1000g (dry weight) of artificial Edgeworthia chrysantha is crushed, 40 meshes are crossed.In eaglewood powder after sizing, addition
3g saccharomyces cerevisiae.In mixture, the water of 2000g is added, controls moisture content 60% or more.By the uniform postposition of material stirring
In hermetic bag, takes out after standing for fermentation 48h in 30 DEG C of constant temperature incubation and dry at room temperature.Fermentation mixing after drying
Supercritical extract was carried out after expecting 40 meshes.Using dehydrated alcohol as entrainer, extracting pressure 28MPa, 46 DEG C of temperature, solid-liquid ratio 1:
1.2, extraction time 2h.Agilawood essential oil extraction yield is 1.47% with this condition.
Claims (10)
1. a kind of processing method for improving agilawood essential oil yield, which comprises the steps of:
(1) after agalloch eaglewood pulverizing and sieving, fresh agalloch eaglewood leaf, saccharomyces cerevisiae and water is added, is mixed evenly, control mixing
The moisture content of material is between 60~90%；
(2) said mixture material is placed in 25~32 DEG C of sealed environment, ferment 48h or more；
(3) by the dry materials after fermentation, supercritical extract is then carried out, obtains agilawood essential oil.
2. preparation method according to claim 1, which is characterized in that the additive amount of step (1) the agalloch eaglewood leaf is heavy
The 5%~25% of incense wood quality.
3. preparation method according to claim 2, which is characterized in that the additive amount of the agalloch eaglewood leaf is agalloch eaglewood quality
10%~20%.
4. preparation method according to claim 1 or 2 or 3, which is characterized in that the addition of step (2) described saccharomyces cerevisiae
Amount is the 0.1%-0.01% of agalloch eaglewood quality.
5. the preparation method according to claim 4, which is characterized in that the additive amount of the saccharomyces cerevisiae is agalloch eaglewood quality
0.03%-0.06%.
6. preparation method according to claim 1 or 2 or 3, which is characterized in that step (2) described supercritical extract is with nothing
Water-ethanol is entrainer.
7. preparation method according to claim 6, which is characterized in that the condition of step (2) described supercritical extract are as follows: extraction
Pressure power 20-30MPa, 40-50 DEG C of temperature, solid-liquid ratio 1:(1~2), 1~3h of extraction time；It is preferred that extracting pressure 28MPa, temperature
46 DEG C, solid-liquid ratio 1:1.2, extraction time 2h of degree.
8. preparation method according to claim 1 or 2 or 3, which is characterized in that step (2) fermentation time be 48~
60h。
9. preparation method according to claim 1 or 2 or 3, which is characterized in that step (1) described agalloch eaglewood smashes it through
40-100 mesh.
10. preparation method according to claim 9, which is characterized in that step (1) described agalloch eaglewood smashes it through 80 mesh
Sieve.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811200667.0A CN109294743A (en) | 2018-10-16 | 2018-10-16 | A kind of processing method improving agilawood essential oil yield |
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| CN201811200667.0A CN109294743A (en) | 2018-10-16 | 2018-10-16 | A kind of processing method improving agilawood essential oil yield |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111286406A (en) * | 2020-03-25 | 2020-06-16 | 浙江黛君生物医药科技有限公司 | Extraction method of eucalyptus essential oil, eucalyptus essential oil and application |
| CN111748407A (en) * | 2020-07-13 | 2020-10-09 | 中国林业科学研究院林产化学工业研究所 | Raw material pretreatment method for extracting cinnamon oil from cinnamon branches and leaves |
| CN113416608A (en) * | 2021-06-29 | 2021-09-21 | 中国热带农业科学院热带作物品种资源研究所 | Extraction method of intelligence-developing essential oil for inhibiting listeria monocytogenes and intelligence-developing essential oil |
-
2018
- 2018-10-16 CN CN201811200667.0A patent/CN109294743A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111286406A (en) * | 2020-03-25 | 2020-06-16 | 浙江黛君生物医药科技有限公司 | Extraction method of eucalyptus essential oil, eucalyptus essential oil and application |
| CN111748407A (en) * | 2020-07-13 | 2020-10-09 | 中国林业科学研究院林产化学工业研究所 | Raw material pretreatment method for extracting cinnamon oil from cinnamon branches and leaves |
| CN113416608A (en) * | 2021-06-29 | 2021-09-21 | 中国热带农业科学院热带作物品种资源研究所 | Extraction method of intelligence-developing essential oil for inhibiting listeria monocytogenes and intelligence-developing essential oil |
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Application publication date: 20190201 |