CN109275560A - A kind of large ocean algae is acidified the system and research method of adaptation Journal of Sex Research for a long time - Google Patents
A kind of large ocean algae is acidified the system and research method of adaptation Journal of Sex Research for a long time Download PDFInfo
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- CN109275560A CN109275560A CN201811472405.XA CN201811472405A CN109275560A CN 109275560 A CN109275560 A CN 109275560A CN 201811472405 A CN201811472405 A CN 201811472405A CN 109275560 A CN109275560 A CN 109275560A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G33/00—Cultivation of seaweed or algae
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
Abstract
A kind of large ocean algae is acidified the system and research method of adaptation Journal of Sex Research for a long time, belongs to Ecological Physiology in Algae field, it includes cultivating workshop and workshop running water system, and within natural waters 1km, workshop roof is translucent glass steel watt for workshop addressing；The workshop running water system adds rich pond and algal culture pond to form by reservoir, carbon dioxide；For water in reservoir quoted from natural waters, reservoir, carbon dioxide add rich pond and algal culture pond three using drainage tube connection, and drainage tube configures valve control inflow and displacement；Carbon dioxide adds Fu Chizhong to be provided with carbon dioxide and adds rich device, adjusts seawater pH value with aeration；The present invention adds the method for rich system Yu natural waters connection by workshop running water carbon dioxide, a kind of metastable macro seawater acidification culture systems are established, is realized using the system and carries out the pilot-scale research that macro is acidified adaptability for a long time under natural conditions.
Description
Technical field
The invention belongs to marine environmental monitoring fields, are acidified adaptability for a long time more particularly to a kind of large ocean algae and grind
The system and research method studied carefully.
Background technique
By the double influence of Global climate change and mankind's activity, carbon dioxide (CO in atmosphere2) discharge amount continues to increase.
The ocean carbon dioxide storage library natural as one about absorbs the mankind discharge total carbon dioxide capacity 30%, causes ocean
Acidification phenomenon.Since the industrial revolution, sea surface pH value has had declined 0.1 unit.By 2100, the value is estimated will be into one
Step 0.3 to 0.4 unit of decline.The increase of seawater acidity will change various balances of sewater chemistry, make dependent on chemical environment
A variety of marine organisms of stability or even the ecosystem face grave danger.In irreversible Ocean acidification process, algae
The adjusting of Physiology and biochemistry and molecular level must be carried out to adapt to the stress being increasingly enhanced.
Large-scale green alga, brown alga and red algae are referred to as " sea grass ", are the chief components of offshore primary productivity, have weight
The carbon sequestration and sewater chemistry system regulatory function wanted；Tangleweed is also the main support person of offshore " submarine forest ", Ke Yiwei
Juvenile fish, invertebrate, coral etc. provide incubator base of and sanctuary.Different genera macro group in the offshore ecosystem
In occupation of different ecological niches, there is different physiological habits, ecological functions are not quite similar.Therefore, carry out Ocean acidification to big
The research of type algological biology response mechanism has important ecological theory meaning and more practical value.
Currently, focusing primarily upon the short-term simulated experiment of laboratory level about the research of macro Ocean acidification, grind
Study carefully discovery, Ocean acidification has positive and negative double effect for the growth and development of macro, and different genera algae answers acidification
Answer that there are significant differences with adaptability.However, more and more research discovery simulating lab tests have limitations, and real
It is shorter to test the period, it is difficult to reflect the physiological adaptation of algae reply Ocean acidification under natural conditions；On the other hand, although a small number of states
Outer scholar carries out long adaptation using natural waters pH difference and studies, it can be difficult to maintaining metastable seawater carbonate body
System, assay reproducibility are poor.
Summary of the invention
The technical problem to be solved by the present invention is to establish a kind of metastable large ocean algae to be acidified adaptability for a long time
Research system and research method.The present invention adds the method for rich system Yu natural waters connection by workshop running water carbon dioxide,
A kind of metastable macro seawater acidification culture systems are established, is realized using the system and is carried out greatly under natural conditions
Type algae is acidified the pilot-scale research of adaptability for a long time.
The present invention is achieved through the following technical solutions:
A kind of large ocean algae is acidified for a long time adapts to Journal of Sex Research system, it includes cultivating workshop and workshop running water system
System；
(1) cultivating workshop: within natural waters 1km, workshop roof is translucent glass steel watt for workshop setting；
The workshop addressing is that workshop is pumped into for the ease of nature seawater within natural waters 1km, realizes that workshop is long
Water system and natural waters connection keep ocean temperature consistent.
It is to realize that sea area tangleweed culture zone illumination is strong that the workshop roof is built using translucent glass steel watt
It spends consistent with workshop running water culture systems culture light intensity.
(2) workshop running water system: workshop running water system includes that reservoir, carbon dioxide add rich pond and algal culture
Pond；Three uses drainage tube connection, and drainage tube configures valve control inflow and displacement；Carbon dioxide adds Fu Chizhong to be provided with
Carbon dioxide adds rich device, adjusts seawater pH value using carbon dioxide plus rich device aeration；
Further, the reservoir length is respectively 4m, 2m, 2m, is pumped seawater from natural waters using immersible pump
To reservoir；The carbon dioxide adds rich pond length to be respectively 4m, 2m, 1m, and seawater introduces carbon dioxide in reservoir
Add Fu Chihou, seawater pH is adjusted using carbon dioxide plus rich device aeration；Algal culture pond length be respectively 1m,
4m, 0.6m, carbon dioxide add the seawater of pH stable in rich pond to enter algal culture pond through drainage tube.
The three uses drainage tube connection, and drainage tube configures valve control inflow and displacement.It is real using this operation
Existing workshop running water system and natural waters connection keep the two water temperature consistent.
The present invention also provides the operation methods of the system, the specific steps are as follows:
(1) algae to be tested is put into algal culture pond；
(2) carbon dioxide, which adds, is arranged different gradient pH in rich pond, the adjustment of pH adds rich device inflation Faville using carbon dioxide
It holds；
(3) add rich pond and algal culture pond three by adjusting drainage tube configuration valve size control reservoir, carbon dioxide
Flowing water speed between person, while the culture density of algae is controlled, to maintain algal culture pond, carbon dioxide to add the pH of Fu Chizhong steady
It is fixed.
Further, when the reservoir length is respectively 4m, 2m, 2m；Carbon dioxide adds rich pond length to distinguish
For 4m, 2m, 1m；When algal culture pond length is respectively 1m, 4m, 0.6m, reservoir, carbon dioxide add rich pond and algae to support
Grow the triangular flowing water speed 2.5L/min in pond；While in order to reduce algae respiration and photosynthesis to the shadow of seawater pH
It rings, algae culture density is no more than 5g FW L-1。
The present invention compared with prior art the utility model has the advantages that
The present invention by build workshop running water be acidified cultivating system carry out macro for a long time be acidified adaptation Journal of Sex Research, 1)
Compared to indoor short-term acidification simulated experiment, present system, which passes through, limits addressing distance and setting translucent glass steel watt ceiling,
Keep the intensity of illumination in system substantially consistent with natural waters；It is cultivated simultaneously using cultivating system running water, and and nature seawater
Connection keeps water temperature in system substantially consistent with natural waters, so that it is long-term to be more truly reflected algae reply under natural conditions
The physiological adaptation of seawater acidification；
2) present invention adds rich device aeration to adjust carbon dioxide and adds seawater pH in rich pond, pass through titanium dioxide by carbon dioxide
Carbon adds the carbon dioxide and air gas mixture of rich device setting certain proportion concentration, and mixed gas adds rich device to be pumped into through carbon dioxide
In seawater, preset pH level is reached by the variation of real-time detection seawater pH value, it is small to maintain default seawater pH variation range
In 0.1.It is tested compared to sea area self-souring sea area, present system can control more accurately and detect seawater pH；
3) Journal of Sex Research offer important references are adapted to the technical scheme is that carrying out macro both at home and abroad and being acidified for a long time,
Gained Physiological Experiment data cope with Future Climate Change model for building marine algae and provide significant data support.
Detailed description of the invention
The workshop Fig. 1 running water is acidified culture systems；
It is horizontal that the workshop Fig. 2 running water difference is acidified culture systems pH；
Kelp cultivation results under Fig. 3 difference acid system；
The workshop Fig. 4 running water system and connection sea area temperature comparisons；
The workshop Fig. 5 running water system and connection sea area light intensity compare.
Specific embodiment
Below by embodiment, the technical solution of the present invention is further explained, but protection scope of the present invention not by
The limitation of embodiment in any form.
Embodiment 1
A kind of large ocean algae is acidified for a long time adapts to Journal of Sex Research system, it includes cultivating workshop and workshop running water system
System；
(1) cultivating workshop: within natural waters 1km, cultivating workshop roof is translucent glass for cultivating workshop setting
Glass steel watt；
The workshop addressing is that workshop is pumped into for the ease of nature seawater within natural waters 1km, realizes that workshop is long
Water system and natural waters connection keep ocean temperature consistent.
It is to realize that sea area tangleweed culture zone illumination is strong that the workshop roof is built using translucent glass steel watt
It spends consistent with workshop running water culture systems culture light intensity.
(2) workshop running water system: workshop running water system includes that reservoir, carbon dioxide add rich pond and algal culture
Pond；Reservoir, carbon dioxide add rich pond and algal culture pond three using drainage tube connection, and drainage tube configures valve control water inlet
Amount and displacement；Carbon dioxide adds Fu Chizhong to be provided with carbon dioxide and adds rich device, adjusts seawater pH value with aeration；
The reservoir length is respectively 4m, 2m, 2m, is pumped seawater to reservoir from natural waters using immersible pump
It is interior；The carbon dioxide adds rich pond length to be respectively 4m, 2m, 1m, and water introducing carbon dioxide adds Fu Chihou in reservoir, adopts
Seawater pH is adjusted with carbon dioxide plus rich device aeration；Algal culture pond length is respectively 1m, 4m, 0.6m, dioxy
Changing carbon adds the seawater of pH stable in rich pond to enter algal culture pond through drainage tube.The three uses drainage tube connection, drainage
Pipe configures valve control inflow and displacement.Using this operation, workshop running water system and natural waters connection are realized, keep
The two water temperature is consistent.
Embodiment 2
Adaptation Journal of Sex Research system is acidified for a long time using large ocean algae constructed by embodiment to be tested
1) experiment selects kelp-kelp (Saccharina japonica) with macro, is derived from and picks up from Shandong
Rongcheng City of province gulf Sang Gou sea area.Selecting scale kelp seedling of the same size is cleared up after the fouling organism of surface for testing.
2) workshop running water acidification system: it is long that workshop is built at the 1km of seashore in Rongcheng City of the Shandong Province gulf Sang Gou sea area
Flowing water acidification system.Workshop running water acidification system is five groups total, becomes for simulating the present and following atmospheric carbon dioxide concentration
400 μ atm, 700 μ atm, 1000 μ atm, 1500 μ atm, 2000 μ atm, five CO are arranged in change trend2Concentration gradient.The long stream in workshop
Water system adds rich pond and algal culture pond to form by reservoir, carbon dioxide.Three uses drainage tube connection, and drainage tube configures valve
Door control inflow and displacement.Carbon dioxide adds Fu Chizhong to add rich device aeration adjustment seawater pH value using carbon dioxide；It stores
Pond length is respectively 4m, 2m, 2m, is pumped seawater to reservoir from natural waters using immersible pump；Carbon dioxide adds
Rich pond length is respectively 4m, 2m, 1m, and water introducing carbon dioxide adds Fu Chihou in reservoir, using carbon dioxide plus rich device
Aeration adjusts seawater pH；Algal culture pond length is respectively 1m, 4m, 0.6m, and carbon dioxide adds pH stable in rich pond
Seawater enter algal culture pond through drainage tube.In order to which the seawater pH for maintaining stable is horizontal, by adjusting drainage in incubation
Pipe configuration valve size control reservoir, carbon dioxide add the triangular flowing water speed in rich pond and algal culture pond to be 2.5L/
min.It uses diameter for the clamping experiment kelp seedling of 0.5cm polyethylene rope, is placed in algal culture pond and cultivates, in order to reduce
The influence of algae respiration and photosynthesis to seawater pH, experiment culture density are 5g FW L-1。
3) workshop running water system seawater pH and the detection of kelp growth traits: experimental period is 6 months, from October, 2017
To in March, 2018.PH level in seawater difference acid system is detected during experiment daily, is monthly measured extra large in different acid systems
Band mean fresh.
4) vehicle workshop running water system and natural waters temperature, intensity of illumination comparison: is detected using Water Test Kits daily
Between running water system and natural waters temperature, calculate monthly average temperature, comparative analysis workshop running water system and natural waters
The difference of temperature；Workshop running water system and natural waters intensity of illumination are detected using light quantum meter daily, calculate every monthly average
Light intensity, the difference of comparative analysis workshop running water system and natural waters intensity of illumination.
Experimental result:
1) workshop running water difference acid system pH changes
400 μ atm, 700 μ atm, 1000 μ atm, 1500 μ atm, 2000 μ atm five are arranged using workshop running water system altogether
A CO2Concentration gradient (such as Fig. 1), Therapy lasted are 6 months total.Difference acidification cultivating systems in pH level variation as shown in Fig. 2,
With CO2The increase of concentration gradient, 8.15 ± 0.03 under pH value is divided by 400 μ atm drop under 2000 μ atm partial pressure
7.52 ± 0.04, significant difference；Another reverse side, with the extension of incubation time, each CO2The variation of pH level is not shown under concentration gradient
It writes, variation range is less than 0.1.
2) kelp cultivation results under different acid systems
In the running water system of workshop under different acidification systems kelp cultivation results as shown in figure 3, prolonging with incubation time
Long, kelp fresh weight dramatically increases under each acid system；With culture CO2Concentration by 400 μ atm, 700 μ atm, 1000 μ atm,
When 1500 μ atm increase to 2000 μ atm, the trend of reduction after first increase is presented in kelp fresh weight, and obtains maximum in 700 μ atm
Value.
3) workshop running water system and connection sea area temperature, light intensity compare
Synchronous detection workshop running water system and natural waters temperature, light intensity, as shown in Figure 4 and Figure 5.Testing result is aobvious
Show, in October, 2017, ocean temperature presentation downward trend was obvious to during in March, 2018, was reduced to by 19.15 ± 0.07 DEG C
3.15±0.07℃.Comparative analysis workshop running water system and connection sea area temperature difference be not significant, and differential magnitude is less than 0.5
℃；During experiment, workshop running water system and natural waters culture intensity of illumination difference is not significant, and averagely culture light intensity is respectively
101±9.4μmol m-2s-1With 103 ± 9.8 μm of ol m-2s-1。
This experiment stops since 2017 10 in March, 2018.It is acidified cultivating system by building workshop running water,
Have studied influence of the long-term seawater acidification to kelp growth and development.Experimental result discovery.Appropriate seawater is acidified (700 μ atm, 1000
μ atm, 1500 μ atm) kelp growth can be remarkably promoted, too strong acidification (2500 μ atm) then inhibits kelp to grow.The long stream in workshop
Water acidification cultivating system compares indoor acidification culture apparatus and significantly extends incubation time, embodies what kelp reply was acidified for a long time
Physiological adaptation；It is tested compared to self-souring sea area, running water acidification cultivating system in workshop can be precisely controlled seawater pH,
PH variation range is less than 0.1；In addition, workshop running water be acidified cultivating system acidification incubation in natural waters connection,
Ocean temperature and culture illumination and natural waters difference is not significant, can more be truly reflected macro under natural conditions and answer
To the physiological adaptation of the following Ocean acidification.
Claims (4)
1. a kind of large ocean algae is acidified for a long time adapts to Journal of Sex Research system, it is characterised in that it includes that cultivating workshop and workshop are long
Water system；
(1) cultivating workshop: within natural waters 1km, workshop roof is translucent glass steel watt for workshop setting；
(2) workshop running water system: workshop running water system includes that reservoir, carbon dioxide add rich pond and algal culture pond；Three
Person uses drainage tube connection, and drainage tube configures valve control inflow and displacement；Carbon dioxide adds Fu Chizhong to be provided with dioxy
Change carbon and add rich device, seawater pH value is adjusted using carbon dioxide plus rich device aeration；The three uses drainage tube connection, drainage tube
Configure valve control inflow and displacement.
2. a kind of large ocean algae according to claim 1 is acidified for a long time adapts to Journal of Sex Research system, it is characterised in that institute
Stating reservoir length is respectively 4m, 2m, 2m, is pumped seawater to reservoir from natural waters using immersible pump；Described two
Carbonoxide adds rich pond length to be respectively 4m, 2m, 1m, and seawater introducing carbon dioxide adds Fu Chihou in reservoir, using dioxy
Changing carbon adds rich device aeration to adjust seawater pH；Algal culture pond length is respectively 1m, 4m, 0.6m, and carbon dioxide adds
The seawater of pH stable enters algal culture pond through drainage tube in Fu Chi.
3. the operation method of system as claimed in claim 1 or 2, it is characterised in that specific step is as follows for the method:
(1) algae to be tested is put into algal culture pond；
(2) carbon dioxide, which adds, is arranged different gradient pH in rich pond, the adjustment of pH adds rich device aeration to maintain using carbon dioxide；
(3) add between rich pond and algal culture pond three by adjusting drainage tube configuration valve size control reservoir, carbon dioxide
Flowing water speed, while the culture density of algae is controlled, to maintain algal culture pond, carbon dioxide that the pH of Fu Chizhong is added to stablize.
4. the operation method of system according to claim 1 or claim 2, it is characterised in that when the reservoir length is respectively
4m,2m,2m；Carbon dioxide adds rich pond length to be respectively 4m, 2m, 1m；Algal culture pond length be respectively 1m, 4m,
When 0.6m, reservoir, carbon dioxide add rich pond and the triangular flowing water speed 2.5L/min in algal culture pond；While in order to reduce
The influence of algae respiration and photosynthesis to seawater pH, algae culture density are no more than 5g FW L-1。
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Application publication date: 20190129 Assignee: Qingdao Xingheyuan Technology Co.,Ltd. Assignor: YELLOW SEA FISHERIES Research Institute CHINESE ACADEMY OF FISHERY SCIENCES Contract record no.: X2022370010022 Denomination of invention: A Study System and Method for Long term Acidification Adaptability of Large Marine Algae Granted publication date: 20210212 License type: Common License Record date: 20221125 |