CN109266545A - A kind of ultrasonic wave DNA interrupts instrument - Google Patents
A kind of ultrasonic wave DNA interrupts instrument Download PDFInfo
- Publication number
- CN109266545A CN109266545A CN201811146862.XA CN201811146862A CN109266545A CN 109266545 A CN109266545 A CN 109266545A CN 201811146862 A CN201811146862 A CN 201811146862A CN 109266545 A CN109266545 A CN 109266545A
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- Prior art keywords
- sink
- ultrasonic wave
- sample disc
- cabinet
- ultrasonic
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
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- General Health & Medical Sciences (AREA)
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- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Crystallography & Structural Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Ultrasonic wave DNA disclosed by the invention interrupts instrument, sample holder assembly for putting centrifuge tube is installed on its cabinet, ultrasonic wave emitting module and control panel are installed in cabinet, the top of cabinet is equipped with sink, sample holder assembly is removably mounted on sink, centrifuge tube is suspended in sink, and ultrasonic wave emitting module connects the bottom that sink is arranged in, and is provided with cooling system in cabinet.This interrupts instrument in use, putting centrifuge tube on sample holder assembly, and water is filled in sink, contacts centrifuge tube with water, can carry out interrupting the non-contact type ultrasonic of sample in centrifuge tube.It is simple, at low cost, widely used that this interrupts instrument structure, mainly for broken, cracking and cell content composition granule the release of cell, by taking scientific research institutions are studied as an example, this instrument is particularly suitable for the particle release of adenovirus, prepare the recombined adhenovirus of high-titer, viral DNA, DNA terminated proteins compound can also be prepared, while being the ideal instrument of pedotheque preparation.
Description
Technical field
The present invention relates to a kind of sample pre-treatments instrument of molecular biology field, specifically a kind of ultrasonic wave DNA is interrupted
Instrument.
Background technique
Traditional DNA is interrupted instrument (i.e. broken instrument) and is interrupted based on instrument with contact, safety, precision, reaction efficiency,
Reacting boundary condition and reaction uniformity aspect, there are certain deficiencies, it is difficult to meet the diversity of experiment type, safety, more
The requirement such as channel, anti-virus cross-infection.
Ultrasonic wave DNA interrupts instrument using non-contact type ultrasonic crushing technology, suitable for the reactant with special requirement
System, compared with conventional contact interrupts instrument, in safety, precision, reaction efficiency, reaction boundary condition and reaction uniformity etc.
The advantage that aspect is had outstanding performance has become the co-precipitation of CHIP(chromatin immune) the indispensable standard chemical industry of research platform
Tool.But current country CHIP laboratory apparatus relies primarily on import, and the DNA of import interrupts instrument two at present: Belgium
The contactless fully-automatic ultrasonic of Bioruptor of Diagenode company development and production is crushed instrument and Qsonica company of the U.S.
The cup type sonicator of production.Due to the technological service after sale of the structure of imported product complexity, fancy price and disconnection
It hinders non-contact type ultrasonic DNA and interrupts instrument in a wide range of popularization and application in China.
Summary of the invention
The technical problem to be solved by the present invention is in view of the deficiencies of the prior art, provide that a kind of structure is simple, cost
Low, widely used ultrasonic wave DNA interrupts instrument.
The technical scheme of the invention to solve the technical problem is: a kind of ultrasonic wave DNA interrupts instrument, including machine
Case is equipped with the sample holder assembly for putting centrifuge tube on the cabinet, and ultrasonic wave transmitting is equipped in the cabinet
Component and control panel, the top of the cabinet are equipped with sink, and the sample holder assembly is removably mounted on described
On sink, the centrifuge tube is suspended in the sink, and the water is arranged in the ultrasonic wave emitting module connection
The bottom of slot is provided with cooling system in the cabinet.
Preferably, the ultrasonic wave emitting module includes ultrasonic transmitter, ultrasonic power plate, several ultrasonic waves
Energy converter and amplitude transformer, the ultrasonic power plate, the ultrasonic transmitter and several ultrasonic transducers
It is sequentially connected electrically, the ultrasonic power plate and the control panel are mounted in a circuit fixed plate, and described is several super
Acoustic wave transducer is fixedly connected with the amplitude transformer respectively, and the amplitude transformer is fixed on the bottom of the sink.
Preferably, the quantity of the ultrasonic transducer is that ultrasonic transducer interval described in three, three is solid
Surely it is connected in the amplitude transformer.
Preferably, the cooling system includes the first blower and the second blower being mounted in the cabinet, institute
The first assembling stated is in the side of several ultrasonic transducers, and second assembling is in the ultrasound
The side wall of the side of wave power panel, the cabinet is equipped with gas-tpe fitting.
Preferably, the sample holder assembly includes the first sample disc, the second sample disc and connecting rod, first sample
Product disk is removably mounted on the upper end of the connecting rod, and second sample disc is removably mounted on the connecting rod
Lower end is provided with slot cover on the sink, and first sample disc is shelved in the slot cover, second sample
Disk is located in the sink, and the outer diameter of first sample disc is greater than the outer diameter of second sample disc, and described the
Several first jacks are offered on a sample disk, offer several second jacks in second sample disc, described first
The aperture of jack is greater than second jack.Second sample disc is used to put the centrifuge tube of small size, needs to assemble large scale
Centrifuge tube when, connecting rod and the second sample disc need to be removed, large-sized centrifuge tube is seated in the first sample disc.First sample
The versatility of instrument can be improved in the design of disk and the second sample disc, meets the assembly needs of different size centrifuge tubes.
Further, the top of the cabinet is equipped with stepper motor, the center of the output end of the stepper motor
The centerline parallel of line and the connecting rod, the output end of the stepper motor are equipped with gear, first sample disc
Circumferential direction be wholely set teeth portion, the gear is meshed with the teeth portion in first sample disc.When work, stepping electricity
Machine drives the first sample disc and the second sample disc spins, and the uniformity to sample treatment can be improved, keep sample broke more thorough.
Further, briquetting moving up and down is arranged on the connecting rod, described is arranged in the briquetting
Between a sample disk and second sample disc, the outer diameter phase of the outer diameter of the briquetting and second sample disc
Match.In the second sample disc put centrifuge tube after, briquetting is pressed on centrifuge tube, is played the role of positioning to centrifuge tube, can prevent from
The buoyancy of heart Guan Yinshui trough inner water is excessive and upper and lower play, incline pendulum, it is ensured that centrifuge tube is steady, is reliably seated in the second sample disc
On.
Further, the slot cover is shelved on the cabinet, and the bottom of the slot cover is fixed with the first bulge loop,
The upper edge of the sink is provided with the flange to turn up, the outer diameter phase of the internal diameter and the flange of first bulge loop
Match, the flange is limited in the inside of first bulge loop, opens up grooved hole, the hole of the slot in the slot cover
Diameter and the outer diameter of first sample disc match, and the second bulge loop for stretching to the slot is fixed in the slot cover,
The internal diameter of second bulge loop is less than the outer diameter of first sample disc, and first sample disc is shelved on described the
On two bulge loops and it is located in the slot.The cooperation of first bulge loop and flange, it can be achieved that mutual limit to slot cover and sink,
The cooperation of second bulge loop and slot simultaneously, can play better positioning action to the first sample disc, and facilitate sample holder assembly
It picks and places.
Preferably, the outside mask of the stepper motor is equipped with motor cover, the top of the connecting rod is from described the
The upper end of a sample disk is stretched out, and the top of the connecting rod is arranged with end cap.
Preferably, be equipped with the first water pump and the second water pump in the cabinet, first water pump and described
The water inlet of sink communicates, and second water pump is communicated with the water outlet of the sink.First water pump and the second water pump are used
In providing recirculated water into sink, avoid sample temperature rise excessively high.
Compared with prior art, the present invention has the advantage that ultrasonic wave DNA disclosed by the invention interrupt instrument in use,
Centrifuge tube is put on sample holder assembly, water is filled in sink, contacts centrifuge tube with water, can be carried out to sample in centrifuge tube
Non-contact type ultrasonic interrupt.It is simple, at low cost, widely used that ultrasonic wave DNA of the present invention interrupts instrument structure, mainly for thin
Broken, cracking and cell content composition granule the release of born of the same parents, by taking scientific research institutions are studied as an example, this instrument is particularly suitable for adenovirus
Particle release, prepare the recombined adhenovirus of high-titer, viral DNA, DNA terminated proteins compound can also be prepared, be simultaneously
The ideal instrument of pedotheque preparation.
Detailed description of the invention
Fig. 1 is the front view that ultrasonic wave DNA interrupts instrument in embodiment;
Fig. 2 is the top view that ultrasonic wave DNA interrupts instrument in embodiment;
Fig. 3 is A-A cross-sectional view in Fig. 2;
Fig. 4 is enlarged drawing at B in Fig. 3;
Fig. 5 is the rearview that ultrasonic wave DNA interrupts instrument in the embodiment for remove cabinet rear portion;
Fig. 6 is the connection schematic diagram of stepper motor and gear in embodiment;
Fig. 7 is effect picture after the centrifuge tube of small size assembles in the second sample disc;
Fig. 8 is effect picture after large-sized centrifuge tube assembles in the first sample disc.
Specific embodiment
The present invention will be described in further detail below with reference to the embodiments of the drawings.
The ultrasonic wave DNA of embodiment 1 interrupts instrument, as shown in Fig. 1~Fig. 6, including cabinet 1, is equipped on cabinet 1 for setting
The sample holder assembly of centrifuge tube 6 is put, ultrasonic wave emitting module and control panel 10 are installed in cabinet 1, the top of cabinet 1 is equipped with
Sink 11, sample holder assembly are removably mounted on sink 11, and centrifuge tube 6 is suspended in sink 11, ultrasonic wave emitting module
The bottom of sink 11 is arranged in connection, is provided with cooling system in cabinet 1.
In embodiment 1, ultrasonic wave emitting module includes 22, three ultrasonic transmitter 21, ultrasonic power plate ultrasonic waves
Energy converter 23 and amplitude transformer 24, ultrasonic power plate 22, ultrasonic transmitter 21 and three ultrasonic transducers 23 are successively electrically connected
It connects, ultrasonic power plate 22 and control panel 10 are mounted in a circuit fixed plate 12, and three intervals of ultrasonic transducer 23 are fixed
It is connected in amplitude transformer 24, amplitude transformer 24 is fixed on the bottom of sink 11.
In embodiment 1, cooling system includes the first blower 31 and the second blower 32 being mounted in cabinet 1, the first blower
31 are mounted on the side of several ultrasonic transducers 23, and the second blower 32 is mounted on the side of ultrasonic power plate 22, cabinet 1
Side wall is equipped with gas-tpe fitting 33.
In embodiment 1, the first water pump 41 and the second water pump 42 are installed in cabinet 1, the first water pump 41 enters with sink 11
The mouth of a river communicates, and the second water pump 42 is communicated with the water outlet of sink 11.
The ultrasonic wave DNA of embodiment 2 interrupts instrument, the difference from embodiment 1 is that, in embodiment 2, sample holder assembly includes
First sample disc 51, the second sample disc 52 and connecting rod 53, the first sample disc 51 are removably mounted in the upper end of connecting rod 53, the
Two sample discs 52, which are removably mounted on the lower end of connecting rod 53, sink 11, is provided with slot cover 13, and the first sample disc 51 is shelved
In slot cover 13, the second sample disc 52 is located in sink 11, and the outer diameter of the first sample disc 51 is greater than the outer diameter of the second sample disc 52,
Several first jacks 54 are offered in first sample disc 51, several second jacks 55 are offered in the second sample disc 52, and first inserts
The aperture in hole 54 is greater than the second jack 55, and briquetting 56 moving up and down is arranged on connecting rod 53, and briquetting 56 is arranged in the first sample
Between product disk 51 and the second sample disc 52, the outer diameter of briquetting 56 and the outer diameter of the second sample disc 52 match.
In embodiment 2, the top of cabinet 1 is equipped with stepper motor 14, the center line of the output end of stepper motor 14 and company
The centerline parallel of bar 53, the output end of stepper motor 14 are equipped with gear 15, and the circumferential direction of the first sample disc 51 has been wholely set
Teeth portion 57, gear 15 are meshed with the teeth portion 57 in the first sample disc 51.The outside mask of stepper motor 14 is equipped with motor cover 16, even
The top of bar 53 is stretched out from the upper end of the first sample disc 51, and the top of connecting rod 53 is arranged with end cap 58.
The ultrasonic wave DNA of embodiment 3 interrupts instrument, the difference from example 2 is that, in embodiment 3, slot cover 13 is shelved on machine
On case 1, the bottom of slot cover 13 is fixed with the first bulge loop 17, and the upper edge of sink 11 is provided with the flange 18 to turn up, the first bulge loop
The outer diameter of 17 internal diameter and flange 18 matches, and flange 18 is limited in the inside of the first bulge loop 17, opens up grooved hole in slot cover 13
59, the aperture of slot 59 and the outer diameter of the first sample disc 51 match, and the second bulge loop for stretching to slot 59 is fixed in slot cover 13
19, the internal diameter of the second bulge loop 19 is less than the outer diameter of the first sample disc 51, and the first sample disc 51 is shelved on the second bulge loop 19 and position
In in slot 59.
Sample holder assembly in above embodiments can assemble various sizes of centrifuge tube 6.Second sample disc 52 is for putting
The centrifuge tube 6 of small size, effect picture is shown in Fig. 7 after assembly, when needing to assemble large-sized centrifuge tube 6, removes connecting rod 53 and the
Large-sized centrifuge tube 6 is seated in the first sample disc 51 by two sample discs 52, and effect picture is shown in Fig. 8 after assembly.
Claims (10)
1. a kind of ultrasonic wave DNA interrupts instrument, including cabinet, the specimen holder group for putting centrifuge tube is installed on the cabinet
Part is equipped with ultrasonic wave emitting module and control panel in the cabinet, it is characterised in that: the top of the cabinet is equipped with
Sink, the sample holder assembly are removably mounted on the sink, and the centrifuge tube is suspended at the sink
Interior, the bottom of the sink is arranged in the ultrasonic wave emitting module connection, is provided with cooling system in the cabinet.
2. a kind of ultrasonic wave DNA according to claim 1 interrupts instrument, it is characterised in that: the ultrasonic wave emitting module
Including ultrasonic transmitter, ultrasonic power plate, several ultrasonic transducers and amplitude transformer, the ultrasonic power plate, institute
The ultrasonic transmitter and several ultrasonic transducers stated are sequentially connected electrically, the ultrasonic power plate and described
Control panel is mounted in a circuit fixed plate, and several ultrasonic transducers are fixedly connected with the amplitude transformer respectively,
The amplitude transformer is fixed on the bottom of the sink.
3. a kind of ultrasonic wave DNA according to claim 2 interrupts instrument, it is characterised in that: the ultrasonic transducer
Quantity is that ultrasonic transducer interval described in three, three is fixedly connected in the amplitude transformer.
4. a kind of ultrasonic wave DNA according to claim 1 interrupts instrument, it is characterised in that: the cooling system includes peace
The first blower and the second blower in the cabinet, first assembling is in several ultrasonic wave transducers
The side of device, second assembling are equipped in the side of the ultrasonic power plate, the side wall of the cabinet
Gas-tpe fitting.
5. a kind of ultrasonic wave DNA according to claim 1 interrupts instrument, it is characterised in that: the sample holder assembly includes
First sample disc, the second sample disc and connecting rod, first sample disc are removably mounted in the upper end of the connecting rod,
Second sample disc, which is removably mounted on, is provided with slot cover on the lower end of the connecting rod, the sink, described
The first sample disc be shelved in the slot cover, second sample disc is located in the sink, first sample
The outer diameter of product disk is greater than the outer diameter of second sample disc, offers several first jacks, institute in first sample disc
Several second jacks are offered in the second sample disc stated, the aperture of first jack is greater than second jack.
6. a kind of ultrasonic wave DNA according to claim 5 interrupts instrument, it is characterised in that: install on the top of the cabinet
There are stepper motor, the centerline parallel of the center line of the output end of the stepper motor and the connecting rod, the stepping
The output end of motor is equipped with gear, and the circumferential direction of first sample disc has been wholely set teeth portion, the gear with it is described
The first sample disc on teeth portion be meshed.
7. a kind of ultrasonic wave DNA according to claim 5 or 6 interrupts instrument, it is characterised in that: be arranged on the connecting rod
Briquetting moving up and down, the briquetting is arranged between first sample disc and second sample disc, described
The outer diameter of briquetting and the outer diameter of second sample disc match.
8. a kind of ultrasonic wave DNA according to claim 5 interrupts instrument, it is characterised in that: the slot cover is shelved on described
Cabinet on, the bottom of the slot cover is fixed with the first bulge loop, and the upper edge of the sink is provided with the flange to turn up, institute
The outer diameter of the internal diameter and the flange of the first bulge loop stated matches, and the flange is limited in the interior of first bulge loop
Side opens up grooved hole in the slot cover, and the aperture of the slot and the outer diameter of first sample disc match, described
Slot cover on be fixed with the second bulge loop for stretching to the slot, the internal diameter of second bulge loop is less than first sample
The outer diameter of disk, first sample disc are shelved on second bulge loop and are located in the slot.
9. a kind of ultrasonic wave DNA according to claim 5 interrupts instrument, it is characterised in that: the outside of the stepper motor
It is covered with motor cover, the top of the connecting rod is stretched out from the upper end of first sample disc, the top set of the connecting rod
Equipped with end cap.
10. a kind of ultrasonic wave DNA according to claim 1 interrupts instrument, it is characterised in that: be equipped in the cabinet
One water pump and the second water pump, first water pump are communicated with the water inlet of the sink, second water pump with it is described
The water outlet of sink communicate.
Priority Applications (1)
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CN201811146862.XA CN109266545A (en) | 2018-09-29 | 2018-09-29 | A kind of ultrasonic wave DNA interrupts instrument |
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CN201811146862.XA CN109266545A (en) | 2018-09-29 | 2018-09-29 | A kind of ultrasonic wave DNA interrupts instrument |
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CN201811146862.XA Pending CN109266545A (en) | 2018-09-29 | 2018-09-29 | A kind of ultrasonic wave DNA interrupts instrument |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110144338A (en) * | 2019-05-29 | 2019-08-20 | 通用生物系统(安徽)有限公司 | It is a kind of using RNA as the RNA polymerase preparation method of template and the application of the polymerase |
CN115287180A (en) * | 2022-08-02 | 2022-11-04 | 江苏百航超声科技有限公司 | Ultrasonic DNA breaks appearance |
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CN110144338A (en) * | 2019-05-29 | 2019-08-20 | 通用生物系统(安徽)有限公司 | It is a kind of using RNA as the RNA polymerase preparation method of template and the application of the polymerase |
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