CN220160210U - Magnetic separator - Google Patents
Magnetic separator Download PDFInfo
- Publication number
- CN220160210U CN220160210U CN202321360145.3U CN202321360145U CN220160210U CN 220160210 U CN220160210 U CN 220160210U CN 202321360145 U CN202321360145 U CN 202321360145U CN 220160210 U CN220160210 U CN 220160210U
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- Prior art keywords
- magnetic
- reagent bottle
- cavity
- ring
- magnetic separator
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- 239000006148 magnetic separator Substances 0.000 title claims abstract description 36
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 72
- 239000011324 bead Substances 0.000 claims abstract description 53
- 238000007885 magnetic separation Methods 0.000 abstract description 16
- 238000009826 distribution Methods 0.000 abstract description 6
- 238000013461 design Methods 0.000 abstract description 4
- 238000000338 in vitro Methods 0.000 abstract description 3
- 238000003745 diagnosis Methods 0.000 abstract description 2
- 238000000926 separation method Methods 0.000 description 9
- 238000004519 manufacturing process Methods 0.000 description 5
- 238000010168 coupling process Methods 0.000 description 3
- 230000002093 peripheral effect Effects 0.000 description 3
- 238000012827 research and development Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000002038 chemiluminescence detection Methods 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 239000006249 magnetic particle Substances 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model relates to the technical field of in-vitro diagnosis, in particular to a magnetic separator, which comprises a shell and a magnetic ring, wherein a cavity is formed in the shell, the cavity is used for accommodating a reagent bottle, magnetic beads and reagents to be separated are arranged in the reagent bottle, the magnetic ring is sleeved outside the shell, the magnetic ring and the reagent bottle placed in the cavity are coaxially arranged, and the magnetic ring provides a magnetic field, so that the magnetic beads in the reagent bottle can be adsorbed on the bottle wall of the reagent bottle. The design that the magnetic force ring surrounds the enclosure body is adopted, so that the magnetic force distribution in the cavity of the enclosure body is uniform and consistent, a uniform magnetic field is formed around the outside of the reagent bottle, the moving speed of the magnetic beads far away from the wall of the reagent bottle is higher, the magnetic beads close to the wall of the reagent bottle are not hardened by the excessively strong magnetic force, the magnetic separation time is shortened, and meanwhile, the magnetic beads at all positions inside the reagent bottle are guaranteed to be uniformly, gently and rapidly recycled, so that the reagent bottle is particularly suitable for magnetic separation of large-volume magnetic bead reagents.
Description
Technical Field
The utility model relates to the technical field of in-vitro diagnosis, in particular to a magnetic separator.
Background
Magnetic beads have unique advantages in biological analysis and separation of proteins, nucleic acids, antibodies and cells, and have been widely used in the field of In Vitro Diagnostics (IVD), magnetic separation being one of the important links. The magnetic separation technology is to make the magnetic beads separate from the non-magnetic substances by means of the magnetic field to make the magnetic beads gather and adsorb the specific substances on the surfaces of the particles under the action of the external magnetic field, so as to capture the substances specifically combined on the surfaces of the magnetic beads and achieve the aim of purification. The magnetic particle type chemiluminescence detection has extremely high sensitivity, extremely high stability and batch-to-batch consistency are required, the requirements on the quality of raw materials are extremely high, and the control of the research and development production process is extremely important. Wherein the coupling process of the magnetic beads and the proteins is extremely important to the performance of the reagent. Magnetic beads with different surface functional groups are different in coupling process. However, a common feature is that the coupling process requires a magnetic separation washing process. How to ensure the separation efficiency of the magnetic beads and the recovery rate of the magnetic beads in the cleaning process is an extremely important parameter. At present, in the small batch preparation process in the research and development stage, research and development personnel can select a simple magnetic rack for magnetic separation. In 2mL or 10mL centrifuge tubes, the magnetic field strength at the far side from the tube wall is not substantially different from that at the near side.
When the reagent enters into the production amplifying stage, the volume of the reagent is amplified to hundreds of milliliters, a few liters or even tens of liters, the simple magnetic frame device is difficult to meet the production requirement. Researchers typically consider that larger gauges use larger and stronger magnets to attract magnetic beads to achieve the same effect in small centrifuge tube experiments, but the problem arises here: because the magnet is bigger and stronger, the magnetic beads close to the magnet part in the reagent bottle can receive extremely strong magnetic force at the beginning of the separation process, and the magnetic force received by the magnetic beads far away from the magnet end is very weak, so that the adsorption time can be longer, and the magnetic beads receiving the excessively strong magnetic force are adsorbed around the bottle wall for a long time, so that the aggregation and hardening of the magnetic beads can be easily generated. In order to solve the problem of hard bead resuspension, attempts have been made to break down the magnetic beads that have accumulated during the separation process by using ultrasound as a solution. However, when the magnetic beads are functionally tested after separation, the aggregated or still aggregated magnetic beads are found to have very different properties from those of the non-aggregated magnetic beads, which can cause large intra-batch variation and inter-batch difference of the reagents, and thus the losses are definitely huge.
The yield drop in a conventional non-uniform magnetic separator when scaled up is mainly due to the magnetic force that varies with the distance of the beads from the magnet. The farther beads are subjected to very low forces and increase rapidly with the area of retention near the magnet. Lower forces can result in slower movement of the beads. When a scaled-up volume is used, the beads move slower and the distance traveled by the beads is longer, which results in longer separation times. If the technician visually inspects the clarification of the suspension after the prescribed separation time has elapsed and chooses to withdraw the supernatant, then the subsequent beads will be lost in large amounts, with the consequent loss of expensive biomolecules.
Disclosure of Invention
In view of the above-mentioned drawbacks of the prior art, the present utility model is directed to providing a magnetic separator with a uniform magnetic force distribution.
In order to solve the technical problems, the utility model adopts the following technical scheme:
the utility model provides a magnetic separator, which comprises a shell and a magnetic ring, wherein a cavity is formed in the shell, the cavity is used for accommodating a reagent bottle, magnetic beads and reagents to be separated are arranged in the reagent bottle, the magnetic ring is sleeved outside the shell and is coaxially arranged with the reagent bottle placed in the cavity, and the magnetic ring provides a magnetic field, so that the magnetic beads in the reagent bottle can be adsorbed on the bottle wall of the reagent bottle.
Preferably, the cavity is a cylindrical cavity with an open upper end and a closed lower end.
Preferably, the cavity is a cylindrical cavity.
Preferably, the inner contour of the cavity matches the outer contour of the reagent bottle.
Preferably, the outer peripheral surface of the shell is a circular ring surface, and the magnetic force ring is a circular ring.
Preferably, the inner ring surface of the magnetic ring is in fit connection with the outer peripheral surface of the shell.
Preferably, the device further comprises a base, and the shell and the magnetic ring are arranged on the base.
Preferably, the shell and the magnetic ring are fixedly connected with the base.
Preferably, the magnetic separator also comprises a handle, wherein the handle is arranged on the magnetic ring and is used for an operator to hold to move the magnetic separator.
Preferably, two handles are provided, and the two handles are symmetrically distributed on two sides of the shell.
Compared with the prior art, the utility model has obvious progress:
the magnetic separator adopts the design that the magnetic ring surrounds the surrounding shell, so that the magnetic force distribution in the cavity of the shell is uniform and consistent, and the magnetic ring and the reagent bottle placed in the cavity of the shell are coaxially arranged, so that a uniform magnetic field is formed around the outside of the reagent bottle, the moving speed of the magnetic beads far away from the bottle wall of the reagent bottle is higher, the magnetic beads close to the bottle wall of the reagent bottle are not hardened by the excessively strong magnetic force, the magnetic separation time is shortened, and the magnetic beads at all positions in the reagent bottle can be uniformly, gently and rapidly recovered. Therefore, the magnetic separator is particularly suitable for magnetic separation of large-volume magnetic bead reagents, can avoid performance difference caused by uneven magnetic force distribution during magnetic separation of the large-volume magnetic bead reagents, reduces the time for magnetic separation of the large-volume magnetic bead reagents, and provides rapid and stable guarantee for producing the large-volume magnetic bead reagents.
Drawings
FIG. 1 is a schematic perspective view of a magnetic separator according to an embodiment of the present utility model.
FIG. 2 is a front view of the magnetic separator shown in FIG. 1.
FIG. 3 is a top view of the magnetic separator shown in FIG. 1.
Wherein reference numerals are as follows:
1. shell body
10. Cavity cavity
2. Magnetic ring
3. Base seat
4. Handle grip
Detailed Description
The following describes the embodiments of the present utility model in further detail with reference to the accompanying drawings. These embodiments are merely illustrative of the present utility model and are not intended to be limiting.
In the description of the present utility model, it should be noted that the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the apparatus or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.
Furthermore, in the description of the present utility model, unless otherwise indicated, the meaning of "a plurality" is two or more.
As shown in fig. 1-3, one embodiment of the magnetic separator of the present utility model.
The magnetic separator of this embodiment includes casing 1 and magnetic force ring 2, and casing 1 inside cavity forms cavity 10, and cavity 10 is used for placing the reagent bottle, and the magnetic bead and the reagent that wait to separate are equipped with in the reagent bottle, and magnetic force ring 2 overcoat is outside casing 1, and magnetic force ring 2 and the coaxial setting of reagent bottle of placing in cavity 10, magnetic force ring 2 provide the magnetic field, make the magnetic bead in the reagent bottle can adsorb on the bottle wall of reagent bottle, realize the magnetic bead separation.
The magnetic separator of this embodiment adopts the design that magnetic force ring 2 encircles and surrounds casing 1 for the magnetic force distribution in casing 1 cavity 10 is even unanimous, and magnetic force ring 2 and place the coaxial setting of reagent bottle in casing 1 cavity 10, thereby form even magnetic field around the reagent bottle, can make the magnetic bead moving speed who keeps away from reagent bottle wall faster, the magnetic bead that is close to reagent bottle wall then is unlikely to receive too strong magnetic force and forms the hardening, both reduced the magnetic separation time, guaranteed simultaneously that the magnetic bead of each position in the reagent bottle all can even, mild, quick recovery. Therefore, the magnetic separator of the embodiment is particularly suitable for magnetic separation of large-volume magnetic bead reagents, can avoid the difference of performances caused by uneven magnetic force distribution during magnetic separation of the large-volume magnetic bead reagents, reduces the time for magnetic separation of the large-volume magnetic bead reagents, and provides rapid and stable guarantee for producing the large-volume magnetic bead reagents.
The magnetic separator of the embodiment can design the volume of the corresponding cavity 10 of the shell 1 according to the different volumes of the magnetic bead production reagents, thereby forming magnetic separators with different volume types and being suitable for various different volumes of the magnetic bead production reagents.
In this embodiment, in order to facilitate the placement and support of the reagent bottles in the cavity 10 of the housing 1, the cavity 10 of the housing 1 is preferably a cylindrical cavity with an open upper end and a closed lower end. Thereby, the reagent bottle can be put into the cavity 10 of the housing 1 from the upper end of the cavity 10 of the housing 1, and the lower end of the reagent bottle can be placed and supported on the lower end of the cavity 10 of the housing 1.
In a preferred embodiment, the cavity 10 of the housing 1 is a cylindrical cavity to accommodate a round reagent bottle.
In this embodiment, in order to ensure the stability of the reagent bottle in the cavity 10 of the housing 1, preferably, the inner contour of the cavity 10 of the housing 1 is matched with the outer contour of the reagent bottle, so that when the reagent bottle is placed in the cavity 10 of the housing 1, the outer contour of the reagent bottle is attached to the inner contour of the cavity 10 of the housing 1. Therefore, the cavity wall of the cavity 10 of the shell 1 can play a role in limiting and supporting the reagent bottle, so that the reagent bottle is stabilized in the cavity 10 of the shell 1, meanwhile, the outer contour of the reagent bottle is attached to the inner contour of the cavity 10 of the shell 1, the interval distance between the reagent bottle and the magnetic ring 2 can be reduced as much as possible, and the magnetic separation time is shortened more advantageously.
In this embodiment, to better adapt to a circular reagent bottle, the outer circumferential surface of the housing 1 is preferably a circular ring, and the magnetic ring 2 is preferably a circular ring. Thereby ensuring that a uniform magnetic field is formed around the outer circumference of the circular reagent bottle.
In this embodiment, the inner ring surface of the magnetic ring 2 is preferably in contact with the outer peripheral surface of the housing 1. This is advantageous, on the one hand, for the mutual fixing of the magnetic ring 2 and the housing 1 and, on the other hand, for the separation distance between the magnetic ring 2 and the reagent bottle in the cavity 10 of the housing 1 to be as small as possible, so that the magnetic separation time is more advantageously shortened.
In order to increase the structural integrity and stability, the magnetic separator of the present embodiment preferably further comprises a base 3, and the housing 1 and the magnetic ring 2 are both disposed on the base 3 and integrated into a unitary structure.
In a preferred embodiment, the housing 1 and the magnetic ring 2 are fixedly connected to the base 3 to form a fixed whole.
Further, the magnetic separator of the embodiment also comprises a handle 4, wherein the handle 4 is arranged on the magnetic ring 2, and the handle 4 is used for an operator to hold to move the magnetic separator so as to facilitate carrying and moving of the magnetic separator.
In a preferred embodiment, two handles 4 are provided, and the two handles 4 are symmetrically distributed on two sides of the casing 1, so that the two hands of an operator can conveniently hold the two handles to move the magnetic separator.
The foregoing is merely a preferred embodiment of the present utility model, and it should be noted that modifications and substitutions can be made by those skilled in the art without departing from the technical principles of the present utility model, and these modifications and substitutions should also be considered as being within the scope of the present utility model.
Claims (10)
1. The utility model provides a magnetic separator, its characterized in that includes casing (1) and magnetic force ring (2), the inside cavity of casing (1) forms cavity (10), cavity (10) are used for placing the reagent bottle, the interior magnetic bead and the reagent of waiting to separate of reagent bottle, magnetic force ring (2) overcoat in casing (1) outside, magnetic force ring (2) with place in the coaxial setting of reagent bottle in cavity (10), magnetic force ring (2) provide the magnetic field, make the magnetic bead in the reagent bottle can adsorb on the bottle wall of reagent bottle.
2. A magnetic separator according to claim 1, characterized in that the cavity (10) is a cylindrical cavity open at the upper end and closed at the lower end.
3. A magnetic separator according to claim 2, characterized in that the cavity (10) is a cylindrical cavity.
4. A magnetic separator according to claim 1, characterized in that the inner contour of the cavity (10) matches the outer contour of the reagent bottle.
5. A magnetic separator according to claim 1, characterized in that the outer circumferential surface of the housing (1) is a torus, and the magnetic ring (2) is a torus.
6. A magnetic separator according to claim 1, characterized in that the inner annular surface of the magnetic ring (2) is in contact with the outer circumferential surface of the housing (1).
7. The magnetic separator according to claim 1, further comprising a base (3), wherein the housing (1) and the magnetic ring (2) are both provided on the base (3).
8. A magnetic separator according to claim 7, characterized in that the housing (1) and the magnetic ring (2) are both fixedly connected to the base (3).
9. The magnetic separator according to claim 8, further comprising a handle (4), said handle (4) being provided on said magnetic ring (2), said handle (4) being intended to be held by an operator for moving said magnetic separator.
10. A magnetic separator according to claim 9, characterized in that two handles (4) are provided, two handles (4) being symmetrically distributed on both sides of the housing (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321360145.3U CN220160210U (en) | 2023-05-31 | 2023-05-31 | Magnetic separator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321360145.3U CN220160210U (en) | 2023-05-31 | 2023-05-31 | Magnetic separator |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220160210U true CN220160210U (en) | 2023-12-12 |
Family
ID=89063300
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321360145.3U Active CN220160210U (en) | 2023-05-31 | 2023-05-31 | Magnetic separator |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220160210U (en) |
-
2023
- 2023-05-31 CN CN202321360145.3U patent/CN220160210U/en active Active
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