CN208829685U - A kind of nucleic acid extraction kit - Google Patents
A kind of nucleic acid extraction kit Download PDFInfo
- Publication number
- CN208829685U CN208829685U CN201821018221.1U CN201821018221U CN208829685U CN 208829685 U CN208829685 U CN 208829685U CN 201821018221 U CN201821018221 U CN 201821018221U CN 208829685 U CN208829685 U CN 208829685U
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- CN
- China
- Prior art keywords
- nucleic acid
- room
- solenoid valve
- washing chamber
- apopore
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Abstract
The utility model discloses a kind of nucleic acid extraction kits, including shell and control panel, case top is provided with liquid feeding lid, control panel side is provided with elution room below liquid feeding lid, elution room side is provided with washing chamber, elution room is connect with washing chamber by pipe method, washing chamber bottom is provided with apopore A, second solenoid valve is provided with below apopore A, third fluid passage is provided with below second solenoid valve, third fluid passage is bolted with washing chamber, the washing chamber other side is provided with magnetic bead storage chamber, magnetic bead storage chamber bottom is provided with apopore B, the first solenoid valve is provided with below apopore B；This nucleic acid extraction kit is provided with cell strainer, cell strainer is connect by buckle with cracking room, it is easy to disassemble, replace the cell strainer of different size, supersonic generator is offered, can be used to lytic cell, may further be used to cleaning equipment after the completion of extracting, control panel and solenoid valve are additionally provided with, it can be achieved that automatic operation.
Description
Technical field
The utility model relates to field of molecular biotechnology, specially a kind of nucleic acid extraction kit.
Background technique
With the increasingly developed maturation of DNA technique, polymerase chain reaction technology is more and more since the eighties emerges
Be applied to molecular biology field, the Protocols in Molecular Biology of nucleic acid be carry out the pathogenic microorganism examination, construct gene pool, object
One of common research means such as kind of origin, diversity evaluation and its affiliation, phyletic evolution, magnetic bead surfaces are connected to can be special
The functional group that ground and DNA have an effect, the characteristic with reversible adsorption DNA is, it can be achieved that automatic operation, greatly reduces examination
The harm to staff is tested, and reduces the particular/special requirement to testing equipment, but current use paramagnetic particle method carries out DNA and mentions
The kit design taken is not reasonable, and it is many inconvenient to exist in use, it is easy to cause during the extraction process because of maloperation
And cause to extract and fail, it is difficult to meet the needs of people.
Utility model content
The purpose of this utility model is to provide a kind of nucleic acid extraction kit, has and can be realized automation, extraction
Nucleic acid purity height, highly concentrated advantage, solve the problems of the prior art.
To achieve the above object, the utility model provides the following technical solutions: a kind of nucleic acid extraction kit, including shell
And control panel, the case top are provided with liquid feeding lid, the liquid feeding side of covering is provided with handle, and the liquid feeding lid lower section is remote
Control panel side is provided with elution room, the elution room side is provided with washing chamber, and the elution room passes through with washing chamber
Pipe method connection, the washing chamber bottom is provided with apopore A, is provided with second solenoid valve below the apopore A, described
It is provided with third fluid passage below second solenoid valve, the third fluid passage is bolted with washing chamber, described to wash
It washs the room other side and is provided with magnetic bead storage chamber, magnetic bead storage chamber bottom is provided with apopore B, sets below the apopore B
It is equipped with the first solenoid valve, is provided with second liquid channel below first solenoid valve, the second liquid channel is deposited with magnetic bead
It puts room to be bolted, the magnetic bead storage chamber other side is provided with cracking room, and cell filter is provided at the top of the cracking room
Net, the cracking room bottom are provided with nucleic acid filter screen, and the nucleic acid filter screen is connected by screw to cracking room, the cracking
Room bottom is provided with jolting plate, and the jolting plate bottom is provided with protective cover, and the protective cover is bolted with jolting plate,
The protective cover is internally provided with ultrasonic transducer, and the ultrasonic transducer side is provided with supersonic generator, described
Supersonic generator and ultrasonic transducer are electrically connected, and the cracking room bottom is provided with the first fluid passage, and described first
Control panel is provided with below fluid passage, the control panel and supersonic generator are electrically connected, the control panel one
Side is provided with power supply line.
Preferably, the liquid feeding lid is connected by hinge with shell.
Preferably, the nucleic acid filter screen is integrally in circular ring shape.
Preferably, the cell strainer specification is 40 microns, and the cell strainer is connected by a snap with cracking room.
Preferably, first fluid passage, second liquid channel and third fluid passage are pressed from both sides with horizontal plane at 15 degree
Angle.
Preferably, the handle is connected by a hinge with liquid feeding lid.
Compared with prior art, the beneficial effects of the utility model are as follows: this nucleic acid extraction kit is provided with cell filter
Net, cell strainer is connect by buckle with cracking room, easy to disassemble, is replaced the cell strainer of different size, is offered ultrasonic wave
Generator can be used to lytic cell, may further be used to cleaning equipment after the completion of extracting, is additionally provided with control panel and solenoid valve, can
Realize automatic operation.
Detailed description of the invention
Fig. 1 is a kind of overall structure cross-sectional view of nucleic acid extraction kit of the utility model；
Fig. 2 is a kind of schematic perspective view of nucleic acid extraction kit of the utility model；
Fig. 3 is a kind of cracking room partial structural diagram of nucleic acid extraction kit of the utility model.
Explanation: 1 cell strainer, 2 jolting plates, 3 nucleic acid filter screens, 4 supersonic generators, 5 ultrasonic wave transducers is marked in figure
Device, 6 first fluid passages, 7 shells, 8 control panels, 9 first solenoid valves, 10 second liquid channels, 11 second solenoid valves, 12
Third fluid passage, 13 elution rooms, 14 washing chambers, 15 magnetic bead storage chambers, 16 cracking rooms, 17 liquid feeding lids, 18 power supply lines, 19 protections
Cover, 20 handles.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment, based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
Every other embodiment obtained, fall within the protection scope of the utility model.
In the description of the present invention, it should be understood that term " on ", "lower", "front", "rear", "left", "right",
The orientation or positional relationship of the instructions such as "top", "bottom", "inner", "outside" is to be based on the orientation or positional relationship shown in the drawings, and is only
For ease of description the utility model and simplify description, rather than the device or element of indication or suggestion meaning must have it is specific
Orientation, be constructed and operated in a specific orientation, therefore should not be understood as limiting the present invention.
Fig. 1-3, a kind of nucleic acid extraction kit, including shell 7 and control panel 8 are please referred to, is provided at the top of shell 7
Liquid feeding lid 17, shell 7 are stainless steel material, and high temperature resistant is corrosion-resistant, and liquid feeding lid 17 is connected by hinge with shell 7, liquid feeding lid 17
Top is provided with handle 20, and handle 20 is connected by a hinge with liquid feeding lid 17, and 17 lower section of liquid feeding lid is set far from 8 side of control panel
It is equipped with elution room 13, elution 13 side of room is provided with washing chamber 14, and elution room 13 is connect with washing chamber 14 by pipe method,
14 bottom of washing chamber is provided with apopore A, is provided with second solenoid valve 11 below apopore A, sets below second solenoid valve 11
It is equipped with third fluid passage 12, third fluid passage 12 is bolted with washing chamber 14, is controlled by second solenoid valve 11
The on-off of apopore A, and then the connected state of elution room 13 and washing chamber 14 is controlled, 14 other side of washing chamber is provided with magnetic bead and deposits
Room 15 is put, 15 bottom of magnetic bead storage chamber is provided with apopore B, is provided with the first solenoid valve 9, the first solenoid valve 9 below apopore B
Lower section is provided with second liquid channel 10, and second liquid channel 10 is bolted with magnetic bead storage chamber 15, and second liquid is logical
Road 10 is connected to washing chamber 14 and magnetic bead storage chamber 15, and the on off operating mode in second liquid channel 10, magnetic are controlled by the first solenoid valve 9
15 other side of pearl storage chamber is provided with cracking room 16, and cell strainer 1 is provided at the top of cracking room 16, and 1 specification of cell strainer is 40
Micron, and cell strainer 1 is connected by a snap with cracking room 16,16 bottom of cracking room is provided with nucleic acid filter screen 3, nucleic acid filtering
Net 3 is connected by screw to cracking room 16, and whole nucleic acid filter screen 3 is in circular ring shape, and 16 bottom of cracking room is provided with jolting plate 2,
Jolting plate 2 is whole rounded, is embedded in the annulus of nucleic acid filter screen 3,2 bottom of jolting plate is provided with protective cover 19, protective cover 19
It is bolted with jolting plate 2, protective cover 19 is internally provided with ultrasonic transducer 5, and 5 side of ultrasonic transducer is provided with
Supersonic generator 4, supersonic generator 4 and ultrasonic transducer 5 are electrically connected, and 16 bottom of cracking room is provided with the first liquid
Channel 6, the first fluid passage 6 are bolted with cracking room 16, and the first fluid passage 6 is connected to cracking room 16 and magnetic bead is stored
Room 15, the first fluid passage 6, second liquid channel 10 and third fluid passage 12 are and horizontal plane is at 15 degree of angles, the first liquid
Control panel 8 is provided with below body channel 6, control panel 8 and supersonic generator 4 are electrically connected, the setting of 8 side of control panel
There is power supply line 18.
Working principle: being put into cracking room 16 by cell strainer 1 for liquid, form single cell suspension, open control panel 8,
Start supersonic generator 4, alternating current is converted into the high-frequency ac electric signal to match with ultrasonic transducer 5, driving ultrasound
Wave transducer 5 works, and ultrasonic transducer 5 converts electrical signals to mechanical energy and jolting plate 2 is driven to generate vibration, emits to slender
In born of the same parents' suspension, when low signal acts on, intensive micro-bubble is generated, when high RST acts on, minute bubbles are burst rapidly, broken using bubble
The shock wave of generation is split to achieve the purpose that smudge cells, and after cell cracking, nucleic acid is led to by nucleic acid filter screen 3 by the first liquid
Road 6 holds room 15 into magnetic bead, and nucleic acid is integrated to magnetic bead surfaces under given conditions, opens the first solenoid valve 9, is integrated to magnetic
The nucleic acid complexes of pearl enter washing chamber 14 by second liquid channel 10, and after nucleic acid complexes are separated by magnetic field, egg is added
White enzyme K, is free in nucleic acid in lysate, then opens second solenoid valve 11, makes the liquid digested by third fluid passage
12 enter elution room 13, carry out isolating and purifying for nucleic acid, after the completion of nucleic acid extraction, open supersonic generator 4, cleaning equipment.
The basic principles and main features of the present invention and the advantages of the present invention have been shown and described above, for
For those skilled in the art, it is clear that the present invention is not limited to the details of the above exemplary embodiments, and without departing substantially from this
In the case where the spirit or essential attributes of utility model, the utility model can be realized in other specific forms；Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the utility model is by institute
Attached claim rather than above description limit, it is intended that will fall within the meaning and scope of the equivalent elements of the claims
All changes are embraced therein, and should not treat any reference in the claims as limiting related right
It is required that.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiments being understood that.
Claims (6)
1. a kind of nucleic acid extraction kit, it is characterised in that: including shell (7) and control panel (8), at the top of the shell (7)
It is provided with liquid feeding lid (17), is provided with handle (20) above the liquid feeding lid (17), far from control below the liquid feeding lid (17)
Panel (8) side is provided with elution room (13), and elution room (13) side is provided with washing chamber (14), the elution room (13)
It is connect with washing chamber (14) by pipe method, washing chamber (14) bottom is provided with apopore A, the lower section apopore A
It is provided with second solenoid valve (11), is provided with third fluid passage (12) below the second solenoid valve (11), the third liquid
Body channel (12) is bolted with washing chamber (14), and washing chamber (14) other side is provided with magnetic bead storage chamber (15),
Magnetic bead storage chamber (15) bottom is provided with apopore B, is provided with the first solenoid valve (9) below the apopore B, and described the
It is provided with second liquid channel (10) below one solenoid valve (9), the second liquid channel (10) and magnetic bead storage chamber (15) are logical
It crosses and is bolted, magnetic bead storage chamber (15) other side is provided with cracking room (16), is provided at the top of the cracking room (16)
Cell strainer (1), cracking room (16) bottom are provided with nucleic acid filter screen (3), the nucleic acid filter screen (3) and cracking room
(16) it is connected by screw to, cracking room (16) bottom is provided with jolting plate (2), and jolting plate (2) bottom is provided with guarantor
Shield (19), the protective cover (19) are bolted with jolting plate (2), and the protective cover (19) is internally provided with ultrasonic wave
Energy converter (5), ultrasonic transducer (5) side are provided with supersonic generator (4), the supersonic generator (4) with
Ultrasonic transducer (5) is electrically connected, and cracking room (16) bottom is provided with the first fluid passage (6), first liquid
Channel (6) is bolted with cracking room (16), is provided with control panel (8) below first fluid passage (6), described
Control panel (8) and supersonic generator (4) are electrically connected, and control panel (8) side is provided with power supply line (18).
2. a kind of nucleic acid extraction kit according to claim 1, it is characterised in that: the liquid feeding lid (17) and shell
(7) it is connected by hinge.
3. a kind of nucleic acid extraction kit according to claim 1, it is characterised in that: the nucleic acid filter screen (3) is whole
In circular ring shape.
4. a kind of nucleic acid extraction kit according to claim 1, it is characterised in that: cell strainer (1) specification is
40 microns, and the cell strainer (1) is connected by a snap with cracking room (16).
5. a kind of nucleic acid extraction kit according to claim 1, it is characterised in that: first fluid passage (6),
Two fluid passages (10) and third fluid passage (12) are and horizontal plane is at 15 degree of angles.
6. a kind of nucleic acid extraction kit according to claim 1, it is characterised in that: the handle (20) and liquid feeding lid
(17) it is connected by a hinge.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821018221.1U CN208829685U (en) | 2018-06-29 | 2018-06-29 | A kind of nucleic acid extraction kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821018221.1U CN208829685U (en) | 2018-06-29 | 2018-06-29 | A kind of nucleic acid extraction kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208829685U true CN208829685U (en) | 2019-05-07 |
Family
ID=66305912
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821018221.1U Expired - Fee Related CN208829685U (en) | 2018-06-29 | 2018-06-29 | A kind of nucleic acid extraction kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208829685U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113462556A (en) * | 2021-09-02 | 2021-10-01 | 德诺杰亿（北京）生物科技有限公司 | Heating assembly of nucleic acid extractor |
-
2018
- 2018-06-29 CN CN201821018221.1U patent/CN208829685U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113462556A (en) * | 2021-09-02 | 2021-10-01 | 德诺杰亿（北京）生物科技有限公司 | Heating assembly of nucleic acid extractor |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190507 Termination date: 20200629 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |