CN204224608U - Multi-functional cell wash-out tank - Google Patents
Multi-functional cell wash-out tank Download PDFInfo
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- CN204224608U CN204224608U CN201420699089.0U CN201420699089U CN204224608U CN 204224608 U CN204224608 U CN 204224608U CN 201420699089 U CN201420699089 U CN 201420699089U CN 204224608 U CN204224608 U CN 204224608U
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- filter screen
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Abstract
The utility model provides a kind of multi-functional cell wash-out tank, dismountable different filter screen and corresponding valve are equipped with in this wash-out tank bottom, efficiently solve cell attachment to cultivate, change the problems such as liquid, digestion, cell washing collection, microcarrier discharge, the cell digested adds new nutrient solution can directly shift amplification.This multi-functional cell wash-out tank comprises bioreactor, filter screen bracket and valve, has valve hole, valve hole is installed the first valve in the middle part of described filter screen bracket; Filter screen bracket at least also has two groups of flutings, wherein one group of fluting is provided with the filter screen filtering microcarrier, is provided with exhaust chamber below this filter screen, bottom exhaust chamber, is provided with outage, and on this outage, the second valve is installed; Another group fluting is provided with the filter screen of filtration cell, is provided with exhaust chamber below filter screen, bottom this exhaust chamber, is also provided with outage, this outage is provided with the 3rd valve.
Description
technical field:
The utility model belongs to biological technical field, relates to a kind of multi-functional cell wash-out tank, and this wash-out tank integrates that cell attachment is cultivated, to change liquid, cell dissociation, cell washing collection, microcarrier discharge etc. multi-functional.
background technology:
Cultivating attached cell is generally in stirring-type bioreactor, by microcarrier suspension culture, the advantage of adherent culture is that cell tight is attached at solid phase surface, directly can be inclined old nutrient solution, new nutrient solution is directly added after cleaning, easy replacing nutrient solution, because cell is fixed on solid phase surface, do not need complicated cell retention system, be convenient to adopt perfusion culture, reach the object improving cell, Secondary Culture will be carried out at the cell of solid dielectric surface-borne for applied solid, digest the operation steps that is absolutely necessary, postdigestive cell adds nutritive medium and makes cell suspending liquid, after inoculation, cell can adherent and increment again.
But carry out digesting very difficult with common bioreactor, cell is often needed to take out from culture tank together with microcarrier, put into special cell wash-out tank to digest, then the cell digested is put into another large tank again and carry out amplification culture, on the other hand, in ordinary cells tank, change liquid cultivates also very difficult, stirring is needed to stop, allow culture tank is static gets off, treat that microcarrier precipitates, again the nutrient solution suction above throw out is fallen, add new nutrient solution again, change liquid so not only not thorough, also can be damaged because of cells quiescent anoxic simultaneously.
summary of the invention:
For solving the problem, the utility model is equipped with dismountable different filter screen and discharge equipment in stirring-type bioreactor bottom, efficiently solve cell attachment (microcarrier) to cultivate, change the problems such as liquid, digestion, cell washing collection, microcarrier discharge, cell adds new nutrient solution can directly shift amplification.
Concrete technical scheme of the present utility model is as follows:
Multi-functional cell wash-out tank, comprises bioreactor, filter screen bracket and valve, has valve hole in the middle part of described filter screen bracket, valve hole is installed the first valve; Filter screen bracket at least also has two groups of flutings, wherein one group of fluting is provided with the filter screen filtering microcarrier, is provided with exhaust chamber below this filter screen, bottom exhaust chamber, is provided with outage, and on this outage, the second valve is installed; Another group fluting is provided with the filter screen of filtration cell, is provided with exhaust chamber below filter screen, bottom this exhaust chamber, is also provided with outage, this outage is provided with the 3rd valve.
The novel further design of this reality is:
Fluting on described filter screen bracket is fan groove, semicircle annular groove, concentric(al) circles annular groove, rectangular tank or polygon groove, the filter screen shape on it and notching construction suitable.
Fluting on described filter screen bracket is preferably two groups.
Described exhaust chamber rectangular in cross-section or V-shaped, outage is located at the bottom of rectangle or V-shaped exhaust chamber.
At stirring-type bioreactor bottom welding or a connection flange, filter screen bracket and bioreactor flange in the bottom are connected as a single entity by fastening piece.
Filter screen bracket adopts stainless steel plate to make, and filter screen and filter screen bracket are adopted and weld or be connected by tackiness agent.
the utility model compared to existing technology tool has the following advantages:
1, the utility model design is ingenious, and gather multi-function in integral whole, changing liquid does not need microcarrier to precipitate, then extracts clear liquid above out, time saving and energy saving, and adopts this dress displacement liquid simple, thorough.
2, when the utility model uses, cell wash-out, collect and do not need to take out from tank, can direct wash-out in tank, collecting cell, can meet different wash-outs, the requirement of collecting cell, directly can carry out cell transfer, production safety, reliably.
3, the utility model solves the bottleneck problem of scale operation attached cell, and microcarrier adherent culture can be made to amplify arbitrarily.
accompanying drawing illustrates:
Fig. 1 is structural representation of the present utility model.
Fig. 2 is the schematic diagram of filter screen bracket and filter screen in the utility model.
Fig. 3 is the structural representation of filter screen bracket in the utility model.
Fig. 4 is one-piece construction schematic diagram of the present utility model.
In figure: 1-second valve; 2-the 3rd valve; 3-first valve; 4-first filter screen; 5-second filter screen; 6-filter screen bracket; 7-valve hole; 8-ring groove; 9-exhaust chamber; 10-bioreactor.
embodiment:
implement one:
In this example, multi-functional cell wash-out tank comprises bioreactor (can adopt stirring-type bioreactor) and valve, and cell cultures pot bottom installs filter screen bracket, and have valve hole in the middle part of this filter screen bracket, the first valve is installed in valve hole.Filter screen bracket also has two groups of concentric semicircles annular grooves, fluting is provided with filter screen, is provided with exhaust chamber, is provided with outage bottom exhaust chamber below this filter screen.Wherein one group of semicircle annular groove is provided with and filters the filter screen of microcarrier, this filter screen cell can pass through, microcarrier not by, have exhaust chamber allow liquid-flow through filter screen below filter screen, below filter screen, bottom land has circular hole, for installing the second valve; Another group semicircle annular groove is provided with the filter screen of filtration cell, this filter screen cell not by, have exhaust chamber allow liquid-flow through filter screen below filter screen, below filter screen, bottom land has circular hole, for installing the 3rd valve.
The exhaust chamber of two groups of concentric semicircles annular groove bottoms, its rectangular in cross-section, outage is located at the bottom of rectangle.
Filter screen bracket adopts stainless steel plate to make, outermost layer be drilled with some apertures for tank body lower part Flange joint, also can not hole, directly and tank body lower part flange be welded.
implement two:
As shown in Figure 1-Figure 3, the multi-functional cell wash-out tank of this example comprises bioreactor 10(and can adopt stirring-type bioreactor), filter screen bracket, and each valve.Have valve hole 7 in the middle part of filter screen bracket, valve hole 7 is installed the first valve 3.
Filter screen bracket 6 also has two groups of annular concentric grooves, wherein interior ring annular groove is provided with the filter screen (the first filter screen 4) filtering microcarrier, exhaust chamber 9 is provided with below this filter screen, outage is provided with bottom exhaust chamber 9, and the second valve 1 is installed on this outage, this filter screen cell can pass through, microcarrier not by, have living space below filter screen the liquid-flow allowed through filter screen.
The ring groove 8 of outer ring is provided with the filter screen (the second filter screen 5) of filtration cell, be provided with exhaust chamber below filter screen, bottom this exhaust chamber, be also provided with outage, this outage is provided with the 3rd valve 2, this filter screen cell not by, have living space below filter screen the liquid-flow allowed through filter screen.
The exhaust chamber 9 of two groups of annular concentric groove bottoms, shape is annular, and its cross section is in the shape of the letter V, and outage is located at the bottom of V-shaped.
Filter screen bracket 6 adopts stainless steel plate to make.Filter screen and filter screen bracket adopt welding process to be connected.At bioreactor bottom welding or a connection flange, filter screen bracket and bioreactor flange in the bottom are connected as a single entity by fastening piece.
One-piece construction of the present utility model as shown in Figure 4.
use procedure of the present utility model is as follows:
Cell is multi-functional cell wash-out tank CIP(incumbent firms before cultivation), SIP(sterilizing in place) after, first valve, second valve, 3rd valve should Close All, cell attachment is cultivated on microcarrier, when midway needs to change liquid, cell can be passed through, intransitable second valve open of microcarrier, nutrient solution and the dead cell be suspended in nutrient solution and non-attached cell, outside filter screen and valve drain tank, if desired thoroughly change old nutrient solution can close the second valve and add PBS and suspend, and then open the second Valved discharge PBS, finally close the second valve, fresh medium is added in tank, again cultivate.
cultivated carry out digesting, collecting cell:
The second valve is opened after cultivation completes, by clean for substratum discharge, close the second valve, the resuspended microcarrier of PBS, then stop stirring, again open the second valve, clear washing lotion, and close the second valve, add pancreatin to stir, peptic cell, after treating that cell dissociation gets off, open cell not by the 3rd valve below filter screen, clear Digestive system, close the 3rd valve, add and stop Digestive system (or washing lotion) Eddy diffusion, carry out cell washing, then the 3rd valve is opened, emptying washing lotion, and close the 3rd valve, add suspension cell protection liquid or nutrient solution, open the second valve and carry out cell packing preservation, or be transferred directly to next cell tank and proceed amplification cultivation.
the collection of microcarrier and discharge:
After cell has shifted, close the second valve, add water suspending carrier, then opens the first valve, carries out collection or the discharge of microcarrier.
Finally carry out CIP and SIP of multi-functional cell wash-out tank, unload filter screen bracket and valve after SIP completes, again clean filter screen and valve by ultrasonic wave, finally, be reinstalled on cell tank, prepare the production of next batch.
The foregoing is only an embodiment of the present utility model, do not form any restriction of the present utility model.All do within spirit of the present utility model and principle any amendment, equivalent to replace and improvement etc., all should be included within protection domain of the present utility model.
Claims (7)
1. multi-functional cell wash-out tank, comprises bioreactor, filter screen bracket and valve, it is characterized in that: have valve hole in the middle part of described filter screen bracket, valve hole is installed the first valve; Filter screen bracket at least also has two groups of flutings, wherein one group of fluting is provided with the filter screen filtering microcarrier, is provided with exhaust chamber below this filter screen, bottom exhaust chamber, is provided with outage, and on this outage, the second valve is installed; Another group fluting is provided with the filter screen of filtration cell, is provided with exhaust chamber below filter screen, bottom this exhaust chamber, is also provided with outage, this outage is provided with the 3rd valve.
2. multi-functional cell wash-out tank according to claim 1, is characterized in that: the fluting on filter screen bracket is fan groove, semicircle annular groove, concentric(al) circles annular groove, rectangular tank or polygon groove, the filter screen shape on it and notching construction suitable.
3. multi-functional cell wash-out tank according to claim 2, is characterized in that: the fluting on described filter screen bracket is two groups.
4. multi-functional cell wash-out tank according to claim 1,2 or 3, is characterized in that: described exhaust chamber rectangular in cross-section or V-shaped, outage is located at the bottom of rectangle or V-shaped exhaust chamber.
5. multi-functional cell wash-out tank according to claim 1, is characterized in that: at stirring-type bioreactor bottom welding or connect a flange, and filter screen bracket and bioreactor flange in the bottom are connected as a single entity by fastening piece.
6. multi-functional cell wash-out tank according to claim 1, is characterized in that: described filter screen bracket adopts stainless steel plate to make.
7. multi-functional cell wash-out tank according to claim 1, is characterized in that: described filter screen and filter screen bracket are adopted and weld or be connected by tackiness agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420699089.0U CN204224608U (en) | 2014-11-20 | 2014-11-20 | Multi-functional cell wash-out tank |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420699089.0U CN204224608U (en) | 2014-11-20 | 2014-11-20 | Multi-functional cell wash-out tank |
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| Publication Number | Publication Date |
|---|---|
| CN204224608U true CN204224608U (en) | 2015-03-25 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201420699089.0U Active CN204224608U (en) | 2014-11-20 | 2014-11-20 | Multi-functional cell wash-out tank |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107400632A (en) * | 2017-08-14 | 2017-11-28 | 广州齐志生物工程设备有限公司 | A kind of cell dissociation amplifying device |
| CN109797101A (en) * | 2017-11-17 | 2019-05-24 | 北京中原合聚经贸有限公司 | A kind of cell reactor microcarrier cell harvest and inoculate amplification method |
-
2014
- 2014-11-20 CN CN201420699089.0U patent/CN204224608U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107400632A (en) * | 2017-08-14 | 2017-11-28 | 广州齐志生物工程设备有限公司 | A kind of cell dissociation amplifying device |
| CN109797101A (en) * | 2017-11-17 | 2019-05-24 | 北京中原合聚经贸有限公司 | A kind of cell reactor microcarrier cell harvest and inoculate amplification method |
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