CN206345856U - Reaction tube - Google Patents
Reaction tube Download PDFInfo
- Publication number
- CN206345856U CN206345856U CN201621316719.7U CN201621316719U CN206345856U CN 206345856 U CN206345856 U CN 206345856U CN 201621316719 U CN201621316719 U CN 201621316719U CN 206345856 U CN206345856 U CN 206345856U
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- Prior art keywords
- reaction tube
- plane
- wedge
- magnet
- pipe
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Abstract
The utility model discloses a kind of reaction tube, it is used for Beads enrichment method seperated nuclear acid, and the reaction tube includes reaction tube and abuts in the magnet of reaction tube lateral wall, and the reaction tube includes：Body, the host cavity limited by body and it is used to and the extraneous mouth of pipe connected, the host cavity, which is used to extract, to react, the mouth of pipe is used for being put into or taking out for mixed liquor, the mouth of pipe is vertically opened up, wherein described body includes at least one plane vertically extended, the plane reclines for magnet, and the magnet is used to adsorb in magnetic-particle to the plane of body in mixed liquor and assembled.The outer wall of reaction tube is made at least one plane, plane carries out face with magnet and contacted, and adds magnetic bead by the area of magnet adsorption, wash area increase, it is not necessary to shake repeatedly, washing step can be simplified, oscillator need not be used, equipment cost is saved, operating efficiency is improved.
Description
Technical field
The utility model is related to technical field of nucleic acid purification, more particularly to a kind of reaction tube for nucleic acid extraction.
Background technology
The existing patent document related to the utility model refers to Chinese patent application disclosed in 18 days April in 2012
No. CN102417902A, and the Chinese Patent No. CN103897987B that on May 4th, 2016 is announced.
It is that, by lysate lysed sample, the nucleic acid molecules discharged from sample are by special that paramagnetic particle method, which extracts nucleic acid,
Magnetic-particle surface is adsorbed onto, and the impurity such as protein is not adsorbed and stayed in the solution.After reaction certain time, then in magnetic
Under field action, magnetic-particle is separated with liquid, reclaim particle (i.e. magnetic bead-DNA conjugates), then washed with cleaning solution i.e. can be with
Obtain pure DNA.Paramagnetic particle method need not centrifuge, need not add plurality of reagents, simple to operate, meet nucleic acid automation extraction
It is required that, it is an important directions of following nucleic acid purification method development.
DNA is separated and purified using Beads enrichment method, simple process is：Sample cracking-magnetic bead with it is to be separated
The washing-magnetic bead separated with DNA to be separated elution (i.e. combination-of DNA specific bindings-magnetic bead-DNA conjugates is washed-washed
De- three steps).
Sample cracking after, magnetic bead is added into lysate, when solution pH value be less than 6.5 when, magnetic bead selectivity with it is excellent
The DNA of change is combined, and now the magnetic bead for being adsorbed with DNA is placed in magnetic field, is removed by buffer solution without adsorbed impurity (egg
It is white etc.), then magnetic bead is placed in the buffer solution of pH value 8.5, the DNA of purifying can enter in buffer solution.
Wherein, the washing process of magnetic bead-DNA conjugates needs to carry out in magnetic bead-DNA conjugate purification systems, generally
Including 96 orifice plates and magnetic frame, 96 orifice plates have 96 reaction tubes for accommodating magnetic bead-DNA conjugates and cleaning solution；The magnetic force
There are frame 96 accommodating chambers to be used for carrying reaction tube, and each accommodating chamber includes at least one rectangular magnet, for giving magnetic bead-DNA
Conjugate provides magnetic field.
As shown in figure 1, the radial cross-section of reaction tube 110 of prior art is circle, contacted in magnetic frame with reaction tube
Magnet 120 is permanent magnet, and surface is plane.As shown in Fig. 2 such reaction tube and the mode of magnet contact contact for point.Such as
Shown in Fig. 3, magnetic bead 130 is radially inside formed straight line during magnet adsorption in reaction tube wall, and magnetic bead is straight along this
Line is assembled, when being washed, and cleaning solution is only capable of washing the outer surface of magnetic bead accumulation regions, and the inside of magnetic bead accumulation regions can not be obtained
To washing, it is therefore desirable to which reaction tube is taken out into magnetic frame, disperseed magnetic bead, due to including miscellaneous inside magnetic bead accumulation regions
Matter, reaction tube is taken out after magnetic frame, and magnetic bead is still bonded together by impurity, it is therefore desirable to which placement is shaken in an oscillator, makes it
It is scattered, then washed, so operate repeatedly repeatedly, until magnetic bead-DNA conjugates are washed clean, carrying out washing separation,
It is cumbersome, in addition it is also necessary to add oscillator, add experimental cost.
Utility model content
Technical problem to be solved in the utility model is：A kind of reaction tube is provided, it can solve magnetic bead washing process
Need to take out reaction tube into the problem of progress magnetic bead disperses and shaken repeatedly.
In order to solve the above technical problems, the technical solution of the utility model is：
A kind of reaction tube, it is used for Beads enrichment method seperated nuclear acid, and the reaction tube includes：Body, limited by body
Host cavity and it is used to and extraneous mouth of pipe connect, mixed liquor of the host cavity for accommodating lysate and magnetic-particle, institute
Stating the mouth of pipe is used for being put into or taking out for mixed liquor, and the mouth of pipe is vertically opened up, wherein the body includes at least one
The plane vertically extended, the plane reclines for magnet.
Further improvement of the utility model is as follows：
Further, the reaction tube includes bottom of the tube, pipe shaft portion and spout part, and the bottom of the tube is small and push up in bottom
The big cone-shaped in end, the pipe shaft portion includes the cylinder cambered surface being connected with plane, and the mouth of pipe is arranged at spout part, and opens upwards
Mouthful.
Further, the reaction tube has four sides, and one pair of which is plane, and other two face is cambered surface or flat
Face.
Further, the reaction tube includes upper and lower part, and the shape of the bottom of the reaction tube is selected from curved surface, wedge
At least one of body, the deformation of wedge, pyramid, bucking ladder, the deformation of bucking ladder.
Further, the distressed structure of the wedge is to be designed to be not parallel to wedge by the line segment parallel with the bottom surface of wedge
The bottom surface of body, so that in bottom of the tube one wedge angle of formation.
Further, the deformation of the wedge is that the parallel line segment in the bottom surface of wedge is designed into angle is less than 180 degree
Two adjacent line segments, so that in bottom of the tube one wedge angle of formation.
It is preferred that, the deformation of the bucking ladder is that the upper bottom of bucking ladder is substituted for into curved surface or triangular pyramid.
It is preferred that, the reaction tube has upper and lower part, the upper end opening, the bottom low side closing, institute
The accommodating chamber that upper and lower part connects and composes liquid is stated, the top has four side walls, and four side walls have four faces,
Four faces are plane.
Further, the bottom includes connecting portion, adsorption section and bottom, and the connecting portion is shaped as bucking ladder, institute
Connecting portion is stated to be used for connecting top and adsorption section.
Further, the adsorption section is used for providing adsorption plane, the absorption cross section when magnetic bead elutes for magnetic bead
Girth be less than top cross section girth.
Compared with prior art, the utility model at least has the advantages that：The outer wall of reaction tube is made at least
One plane, plane carries out face with magnet and contacted, and adds magnetic bead by the area of magnet adsorption, washing area increase, it is not necessary to
Shake repeatedly, washing step can be simplified, it is not necessary to use oscillator, save equipment cost, simplify processing procedure, improved
Operating efficiency.
Brief description of the drawings
Fig. 1 is the reaction tube of prior art and the schematic elevation view of magnet.
Fig. 2 is reaction tube as shown in Figure 1 and magnet linear contact lay schematic diagram.
Fig. 3 is magnetic bead washing schematic diagram as shown in Figure 1.
Fig. 4 is the reaction tube of the utility model first embodiment and the schematic elevation view of magnet.
Fig. 5 is reaction tube as shown in Figure 4 and magnet face contact schematic diagram.
Fig. 6 is magnetic bead washing schematic diagram as shown in Figure 4.
Fig. 7 is the reaction tube of the utility model second embodiment and the schematic diagram of electromagnet.
Fig. 8-Figure 13 is the structural representation of the reaction tube of the utility model 3rd embodiment.
Figure 14 is the reaction tube of the utility model fourth embodiment and the schematic diagram of electromagnet.
Embodiment
The utility model is further illustrated with reference to the accompanying drawings and examples.
As shown in Figs. 4-6, to meet first embodiment of the present utility model.A kind of reaction tube 10, it is used for Beads enrichment
Method seperated nuclear acid.
Reaction tube 10 includes：Body 11, the host cavity 12 limited by body 11 and it is used to and the extraneous mouth of pipe connected
13.The host cavity 12 is used for the mixed liquor 40 for accommodating lysate and magnetic-particle 30, and the mouth of pipe 13 is used for mixed liquor 40
It is put into or takes out, the mouth of pipe 13 is vertically opened up, the body includes at least one plane vertically extended
14, the plane 14 reclines for magnet 20.
Magnet 20 is permanent magnet.The magnet 20 is in rectangular-shape or cylindric, and with the plane 14 with reaction tube 10
The interface (non-label) of abutting contact.Reaction tube 10 includes bottom of the tube 15, pipe shaft portion 16 and spout part 17, the bottom of the tube
15 be in the cone-shaped that bottom is small and top is big, and the pipe shaft portion 16 includes the cylinder cambered surface 161 being connected with plane, the mouth of pipe 13
It is arranged at spout part 17, and upward opening.The angular range of the cross section circular arc of reaction tube 10 is 60 degree to 300 degree.
As shown in fig. 7, to meet second embodiment of the present utility model, reaction tube 40 has four sides, one pair of which
For plane 43, other two face is cambered surface 44.Reaction tube 200 includes top 41 and bottom 42, such as Fig. 8-Figure 13 the present embodiment institute
The shape of bottom 42 of reaction tube is stated in curved surface, wedge, the deformation of wedge, pyramid, bucking ladder, the deformation of bucking ladder
It is any.
The distressed structure of the wedge is the bottom surface that the line segment parallel with the bottom surface of wedge is designed to be not parallel to wedge,
So as in bottom of the tube one wedge angle of formation.After the completion of elution, on the inwall using magnet absorption magnetic bead to the bottom of the tube, remove
Liquid, when content liquid is less, the accommodating chamber that the liquid accumulation is formed to the wedge angle, the magnetic bead absorption is in bottom of the tube
On inwall away from wedge angle, liquid and magnetic bead can be separated, it is convenient to remove whole liquid.
As one kind deformation of the present embodiment, the deformation of the wedge is that the parallel line segment in the bottom surface of wedge is designed into folder
Angle is less than two adjacent line segments of 180 degree, so that in bottom of the tube one wedge angle of formation.After the completion of elution, magnet absorption is used
Magnetic bead removes liquid on the inwall of the bottom of the tube any plane, when content liquid is less, and the liquid accumulation is described in
The accommodating chamber of wedge angle formation, is realized liquid and Beads enrichment, convenient to remove whole liquid.
The deformation of the bucking ladder, is that the upper bottom of bucking ladder is substituted for into curved surface or triangular pyramid.Two electromagnet 45 are distinguished
Inhale in two planes 43.
As shown in figure 14, to meet fourth embodiment of the present utility model.Reaction tube 60 has top 61 and bottom 62, institute
The top end opening of top 61 is stated, the low side of bottom 62 closing, the top 61 and bottom 62 connect and compose the accommodating chamber 63 of liquid,
The top 61 has four side walls 64, and four side walls have four faces, and four faces are plane.The bottom 62
Including connecting portion 65, adsorption section 66 and bottom 67, the connecting portion 65 is shaped as bucking ladder, and the connecting portion 65 is used for connecting
Top 62 and adsorption section 66.The adsorption section 66 is used for providing adsorption plane when magnetic bead elutes for magnetic bead, and the adsorption section 66 is horizontal
The girth in section is less than the girth of the cross section of top 61.The bottom 67 is class trapezoidal column, class round platform, cone or reverse pyramid
At least one of.Post other two in the two side posted on two faces in side wall 64 on two electromagnet 68, adsorption section 66
Individual electromagnet 68.
Compared with prior art, the utility model has the advantages that：
(1) outer wall of reaction tube is made at least one plane, plane carries out face with magnet and contacted, and adds magnetic bead by magnetic
The area of iron absorption, washing area increase, it is not necessary to shake repeatedly, washing step can be simplified, it is not necessary to use oscillator, save
Equipment cost, simplifies processing procedure, improves operating efficiency
(2) connecting portion is shaped as bucking ladder, and the connecting portion is used for connecting top and adsorption section, convenient absorption exists
The magnetic bead on top can smoothly be slipped to bottom after magnetic force attraction is cancelled；
(3) adsorption section is used for providing adsorption plane, the girth of the absorption cross section when magnetic bead elutes for magnetic bead
Less than the girth of top cross section, when magnetic bead completes to rinse, magnetic bead is slipped to behind bottom, it is only necessary to add a small amount of eluent
(liquid level reaches the connecting line of adsorption section and connecting portion) coordinates the blocked operation of the 3rd magnet and the 4th magnet, so that it may
The elution of magnetic bead-DNA conjugates is rapidly completed, eluent consumption is saved, improves elution efficiency.
(4) the reaction tube bottom is shaped as curved surface, wedge, the deformation of wedge, pyramid, bucking ladder, bucking ladder
Any of deformation, when content liquid is less, can conveniently siphon away liquid by liquid accumulation in the minimum point of bottom.
The utility model is not limited to above-mentioned specific embodiment, and one of ordinary skill in the art visualizes from above-mentioned
Hair, without performing creative labour, made a variety of conversion are all fallen within protection domain of the present utility model.
Claims (11)
1. a kind of reaction tube, it is used for Beads enrichment method seperated nuclear acid, and the reaction tube includes：Body, the receipts limited by body
Cavity volume and be used to the mouth of pipe that connect of the external world, the host cavity is described for providing the sample treatment in nucleic acid separation process
The mouth of pipe is vertically opened up, it is characterised in that：The body includes at least one plane vertically extended, the plane
Reclined for magnet.
2. the reaction tube as described in claim 1, it is characterised in that：The reaction tube includes bottom of the tube, pipe shaft portion and pipe
Oral area, the bottom of the tube is in the cone-shaped that bottom is small and top is big, and the pipe shaft portion includes the cylinder cambered surface being connected with plane, institute
State the mouth of pipe and be arranged at spout part, and upward opening.
3. the reaction tube as described in claim 1, it is characterised in that：The reaction tube has four sides, and one pair of which is
Plane, other two face is cambered surface or plane.
4. the reaction tube as described in claim 3, it is characterised in that：The reaction tube includes upper and lower part, the reaction
The shape of the bottom of pipe in curved surface, wedge, the deformation of wedge, pyramid, bucking ladder, the deformation of bucking ladder at least one
Kind.
5. the reaction tube as described in claim 4, it is characterised in that：The distressed structure of the wedge is by the bottom surface with wedge
Parallel line segment is designed to be not parallel to the bottom surface of wedge, so that in bottom of the tube one wedge angle of formation.
6. the reaction tube as described in claim 4, it is characterised in that：The deformation of the wedge is that the bottom surface of wedge is parallel
Line segment is designed to that angle is less than two adjacent line segments of 180 degree, so that in bottom of the tube one wedge angle of formation.
7. reaction tube as claimed in claim 4, it is characterised in that the deformation of the bucking ladder, is to replace the upper bottom of bucking ladder
Change curved surface or triangular pyramid into.
8. the reaction tube as described in claim 4, it is characterised in that：The reaction tube has upper and lower part, the top
Top end opening, the bottom low side closing, the upper and lower part connects and composes the accommodating chamber of liquid, and the top has four
Side wall, four side walls have four faces, and four faces are plane.
9. the reaction tube as described in claim 8, it is characterised in that：The bottom includes connecting portion, adsorption section and bottom, institute
The bucking ladder that is shaped as of connecting portion is stated, the connecting portion is used for connecting top and adsorption section.
10. the reaction tube as described in claim 9, it is characterised in that：The adsorption section is used for when magnetic bead is eluted being magnetic bead
Adsorption plane is provided, the girth of the absorption cross section is less than the girth of top cross section.
11. the reaction tube as described in claim 1, it is characterised in that：The magnet is permanent magnet or electromagnet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621316719.7U CN206345856U (en) | 2016-12-02 | 2016-12-02 | Reaction tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621316719.7U CN206345856U (en) | 2016-12-02 | 2016-12-02 | Reaction tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206345856U true CN206345856U (en) | 2017-07-21 |
Family
ID=59318728
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621316719.7U Expired - Fee Related CN206345856U (en) | 2016-12-02 | 2016-12-02 | Reaction tube |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206345856U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113528333A (en) * | 2021-07-20 | 2021-10-22 | 北京擎科生物科技有限公司 | Nucleic acid synthesis reaction apparatus and nucleic acid synthesis method |
-
2016
- 2016-12-02 CN CN201621316719.7U patent/CN206345856U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113528333A (en) * | 2021-07-20 | 2021-10-22 | 北京擎科生物科技有限公司 | Nucleic acid synthesis reaction apparatus and nucleic acid synthesis method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170721 Termination date: 20191202 |