CN205635571U - A mixing device that is used for paramagnetic particle method nucleic acid extraction appearance and protein purification appearance - Google Patents
A mixing device that is used for paramagnetic particle method nucleic acid extraction appearance and protein purification appearance Download PDFInfo
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- CN205635571U CN205635571U CN201620477994.0U CN201620477994U CN205635571U CN 205635571 U CN205635571 U CN 205635571U CN 201620477994 U CN201620477994 U CN 201620477994U CN 205635571 U CN205635571 U CN 205635571U
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- bar magnet
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Abstract
The utility model relates to a mixing device that is used for paramagnetic particle method nucleic acid extraction appearance and protein purification appearance, includes: the cover pipe holder, the movable support that shakes, its below that lies in set pipe holder is connected through the suspension nut with set pipe holder, the movable support that shakes is equipped with a plurality of detachable sleeve pipes, the horizontal vibration motor, it is connected with the movable support that shakes, the vertical vibration motor, it is connected with the movable support that shakes, the bar magnet support, its top that lies in set pipe holder, fixedly connected with a plurality of bar magnet on the bar magnet support, wherein, be equipped with the jack that is used for the bar magnet to alternate on the cover pipe holder, the bar magnet support is through reciprocating in the bar magnet inserted casing. The present case realizes different rates, two kinds of vibration way of different ranges, multiple vibration range and vibration frequency's arbitrary combination under two vibrating motor's effect, carry out quick effectual mixing with magnetic bead and liquid, and reciprocating of the bar magnet of deuterogamying has realized the quick transfer of magnetic bead.
Description
Technical field
This utility model relates to a kind of mixing parts, particularly to one for paramagnetic particle method instrument for extracting nucleic acid and
The evenly mixing device of protein purification instrument.
Background technology
Nanometer magnetic bead, due to its bigger serface and the character of modified surface difference in functionality chemical group,
And under externally-applied magnetic field, himself can carry out the spy separated easily with operation mother solution with superparamagnetism
Property, it is widely used at nucleic acid and protein extraction field.In order to improve extraction efficiency and carry out high flux and carry
Take, usually coordinate automatic nucleic acid extraction apparatus or protein purification instrument to use.At present, core is extracted at paramagnetic particle method
During acid or purifying protein, mainly there are following 2 deficiencies when coordinating instrumentation: (one) magnetic bead exists
Liquid mix insufficient.Needing combining, during washing and eluting of nucleic acid or albumen and magnetic bead
Be well dispersed among the system of liquid by magnetic bead, currently used method is mainly single direction machine
Tool clashes into mixing, i.e. vertical sample liquid level direction and clashes into sample liquids mixing up and down by sleeve pipe.Due to only
There is the shock mixing in a direction, the blending efficiency of sample is relatively low, generally requires higher mixing intensity
Longer mixing time, on the one hand this blending manner extends extracts and the time of purification, the opposing party
In the face of the requirement of strength of equipment is higher, can not get the mechanical life of equipment well ensureing.Additionally, it is single
One direction mechanical impact mixing is poor to micro liquid Blending Efficiency of Blending, is especially processing the complex samples such as blood
There is magnetic bead knot tying phenomenon in Shi Jingchang, it is impossible to realize fine dispersion.
Along with the development of extraction from biological material purification technique, particularly automatic nucleic acid extracts or albumen is pure
Change the development of instrument, be badly in need of a kind of new to be applied to paramagnetic particle method instrument for extracting nucleic acid and egg more efficiently
The evenly mixing device of Bai Chunhua instrument solves the problems referred to above.
Utility model content
The deficiency existed for prior art, this case provides a kind of for paramagnetic particle method instrument for extracting nucleic acid and egg
The evenly mixing device of Bai Chunhua instrument, this device can provide the interference vibration mixing of multiple directions, i.e. normally
Under conditions of rule vibration, introduce the vibration of different directions, amplitude (size, the side of interference normal vibration
To), thus destroy float stable state in the liquid phase, reach the purpose of the most effectively mixing.
For achieving the above object, this case is achieved through the following technical solutions:
A kind of for paramagnetic particle method instrument for extracting nucleic acid with the evenly mixing device of protein purification instrument, comprising:
Casing support；
Vibration rack, it is positioned at the lower section of described casing support, with described casing support by suspension nut
Connect；Described vibration rack is provided with multiple dismountable sleeve pipe；
Horizontal vibration motor, it is fixed on the side of described vibration rack, and with described vibration rack transmission
Connect；
Vertical vibration motor, it is fixed on the side of described vibration rack, and with described vibration rack transmission
Connect；
Bar magnet support, it is positioned at the top of described casing support, if being fixedly connected with on described bar magnet support
Dry bar magnet；
Wherein, described casing support is provided with the jack interted for described bar magnet；Described bar magnet is with described
Sleeve pipe one_to_one corresponding, described bar magnet is inserted in described sleeve pipe by described bar magnet support by moving up and down.
Preferably, described for paramagnetic particle method instrument for extracting nucleic acid and the evenly mixing device of protein purification instrument, its
In, described vibration rack is provided with the slot of fixing described sleeve pipe.
The beneficial effects of the utility model are: this case is common horizontal vibration motor and vertical vibration motor
Under effect, it is achieved different rates, different two kinds of mode of vibrations of amplitude, multiple Oscillation Amplitude and vibration frequency
The combination in any of rate, can mix magnetic bead and liquid fast and effectively, then coordinate the upper and lower of bar magnet
Move freely, it is achieved magnetic bead absorption under diverse location, different rates, different vibrational state and release
Action, realizes the transfer of magnetic bead fast and effectively；Overcome common paramagnetic particle method instrument for extracting nucleic acid or albumen is pure
Change bar magnet in instrument to impact magnetic bead in liquid phase the most up and down and be unable to the defect making magnetic bead fully mix.
Accompanying drawing explanation
Fig. 1 is for paramagnetic particle method instrument for extracting nucleic acid and the structural representation of the evenly mixing device of protein purification instrument.
Detailed description of the invention
Below in conjunction with the accompanying drawings this utility model is described in further detail, to make those skilled in the art
Can implement according to this with reference to description word.
As it is shown in figure 1, this case list an embodiment for paramagnetic particle method instrument for extracting nucleic acid and protein purification instrument
Evenly mixing device, comprising:
Casing support 1；
Vibration rack 2, it is positioned at the lower section of casing support 1, with casing support 1 by suspension nut 3
Connect；Vibration rack 2 is provided with multiple dismountable sleeve pipe 4；
Horizontal vibration motor 5, it is fixed on the side of casing support 1, and connects with vibration rack 2 transmission
Connect；
Vertical vibration motor 6, it is fixed on the side of casing support 1, and connects with vibration rack 2 transmission
Connect；
Bar magnet support 7, it is positioned at the top of casing support 1, bar magnet support 7 is fixedly connected with some
Individual bar magnet 8；
Wherein, casing support 1 is provided with the jack 9 interted for bar magnet 8；Bar magnet 8 and sleeve pipe 4 one
One is corresponding, and bar magnet 8 is inserted in sleeve pipe 4 by bar magnet support 7 by moving up and down.
In the above-described embodiments, vibration rack 2 is additionally provided with the slot 10 of fixed sleeving 4.
Suspension nut 3 can make the vibration of horizontal vibration motor 5 and vertical vibration motor 6 cannot conduct to
On casing support 1.Whole evenly mixing device is typically fixed by casing support 1 as a fixing end.
When sleeve pipe 4 has just arrived sample liquid level, opening horizontal vibration motor 5, in liquid, magnetic bead is uniformly dispersed,
When sleeve pipe 4 arrives bottom liquid level, open vertical vibration motor 6 and adjust frequency, at two vibration electricity
The magnetic bead being gathered in bottom under the common effect of machine has obtained good dispersion mixing.When magnetic bead collected by needs
Time, bar magnet 8 is moved to sleeve pipe 4, thus realizes the collection to magnetic bead.
Although embodiment of the present utility model is disclosed as above, but it is not restricted to description and reality
Executing listed in mode utilization, it can be applied to various applicable field of the present utility model completely, for
For those skilled in the art, it is easily achieved other amendment, therefore without departing substantially from claim
And under the general concept that limited of equivalency range, this utility model is not limited to specific details and shows here
The legend gone out and describe.
Claims (2)
1. one kind is used for paramagnetic particle method instrument for extracting nucleic acid and the evenly mixing device of protein purification instrument, it is characterised in that
Including:
Casing support；
Vibration rack, it is positioned at the lower section of described casing support, with described casing support by suspension nut
Connect；Described vibration rack is provided with multiple dismountable sleeve pipe；
Horizontal vibration motor, it is fixed on the side of described vibration rack, and with described vibration rack transmission
Connect；
Vertical vibration motor, it is fixed on the side of described vibration rack, and with described vibration rack transmission
Connect；
Bar magnet support, it is positioned at the top of described casing support, if being fixedly connected with on described bar magnet support
Dry bar magnet；
Wherein, described casing support is provided with the jack interted for described bar magnet；Described bar magnet is with described
Sleeve pipe one_to_one corresponding, described bar magnet is inserted in described sleeve pipe by described bar magnet support by moving up and down.
2. the mixing dress for paramagnetic particle method instrument for extracting nucleic acid and protein purification instrument as claimed in claim 1
Put, it is characterised in that described vibration rack is provided with the slot of fixing described sleeve pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620477994.0U CN205635571U (en) | 2016-05-24 | 2016-05-24 | A mixing device that is used for paramagnetic particle method nucleic acid extraction appearance and protein purification appearance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620477994.0U CN205635571U (en) | 2016-05-24 | 2016-05-24 | A mixing device that is used for paramagnetic particle method nucleic acid extraction appearance and protein purification appearance |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205635571U true CN205635571U (en) | 2016-10-12 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620477994.0U Active CN205635571U (en) | 2016-05-24 | 2016-05-24 | A mixing device that is used for paramagnetic particle method nucleic acid extraction appearance and protein purification appearance |
Country Status (1)
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|---|---|
| CN (1) | CN205635571U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107267501A (en) * | 2017-07-21 | 2017-10-20 | 杭州奥盛仪器有限公司 | Segmented absorption magnetic bead method, storage device and nucleic acid extracting instrument |
| CN109609346A (en) * | 2019-02-14 | 2019-04-12 | 盈开生物科技（上海）有限公司 | A kind of method and apparatus based on paramagnetic particle method nucleic acid extraction |
-
2016
- 2016-05-24 CN CN201620477994.0U patent/CN205635571U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107267501A (en) * | 2017-07-21 | 2017-10-20 | 杭州奥盛仪器有限公司 | Segmented absorption magnetic bead method, storage device and nucleic acid extracting instrument |
| CN109609346A (en) * | 2019-02-14 | 2019-04-12 | 盈开生物科技（上海）有限公司 | A kind of method and apparatus based on paramagnetic particle method nucleic acid extraction |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20170906 Address after: High tech park and Jiangning Road District of Nanjing City, Jiangsu Province, No. 18 211100 Patentee after: Nanjing Ying Rui Cheng song biochemical instrument Co., Ltd. Address before: 215500 Changshu City, Jiangsu province high tech Industrial Development Zone, Southeast Avenue, No. 1, building 68, No. Patentee before: Suzhou Ying Ruicheng biochemical technology Co., Ltd |
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| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20211104 Address after: 215522 No. 8, Shengyu Avenue, Haiyu Town, Changshu City, Suzhou City, Jiangsu Province Patentee after: SUZHOU ENRICHING BIOTECHNOLOGY CO.,LTD. Address before: 211100 No.18 Zhilan Road, gaoxinyuan, Jiangning District, Nanjing City, Jiangsu Province Patentee before: NANJING ENRICHING MAIGE BIOCHEMISTRY APPARATUS CO.,LTD. |
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| TR01 | Transfer of patent right |