CN206345848U - Magnetic frame - Google Patents
Magnetic frame Download PDFInfo
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- CN206345848U CN206345848U CN201621316734.1U CN201621316734U CN206345848U CN 206345848 U CN206345848 U CN 206345848U CN 201621316734 U CN201621316734 U CN 201621316734U CN 206345848 U CN206345848 U CN 206345848U
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- electromagnet
- reaction tube
- magnetic
- magnetic frame
- plane
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Abstract
The utility model discloses a kind of magnetic frame, it is used for Beads enrichment method seperated nuclear acid, and the magnetic frame includes support body and reaction tube；The support body includes panel, the supporting plate being arranged at below panel and for support panel and the side plate of supporting plate, and the panel is provided with some through holes, and the reaction tube is placed in the supporting plate through the through hole；The magnetic frame also includes electromagnet assembly, the electromagnet assembly is arranged on the side wall of the supporting plate or the support body, the body includes at least one plane vertically extended, the plane reclines for electromagnet assembly, and the electromagnet assembly is used to adsorb in magnetic bead to the plane of body in mixed liquor and assembled.Magnetic frame provides magnetic field using electromagnet, can freely control in the change in magnetic field, washing process by controlling electromagnet just to realize the aggregation of magnetic bead and disperse, it is not necessary to reaction tube is taken out into field regions, the automaticity of whole nucleic acid purification process is improved.
Description
Technical field
The utility model is related to technical field of nucleic acid purification, more particularly to a kind of magnetic frame for nucleic acid extraction.
Background technology
The existing patent document related to the utility model refers to Chinese patent application disclosed in 18 days April in 2012
No. CN102417902A, and the Chinese Patent No. CN103897987B that on May 4th, 2016 is announced.
It is that the nucleic acid molecules of separate out are by special from sample by lysate lysed sample that paramagnetic particle method, which extracts nucleic acid,
Magnetic-particle surface is adsorbed onto, and the impurity such as protein is not adsorbed and stayed in the solution.After reaction certain time, then in magnetic
Under field action, magnetic-particle is separated with liquid, reclaim particle (i.e. magnetic bead-DNA), then washed with cleaning solution and can obtain pure
Net DNA.Paramagnetic particle method need not centrifuge, need not add plurality of reagents, simple to operate, meet nucleic acid automation and extract requirement,
It is an important directions of following nucleic acid purification method development.
DNA is separated and purified using Beads enrichment method, simple process is：Sample dissociation-magnetic bead with it is to be separated
DNA specific bindings-magnetic bead-DNA washing-magnetic bead separates (i.e. combination-washing-elution three with DNA to be separated elution
Step).
Sample cracking after, magnetic bead is added into lysate, when solution pH value be less than 6.5 when, magnetic bead selectivity with it is excellent
The DNA of change is combined, and now the magnetic bead for being adsorbed with DNA is placed in magnetic field, is removed by buffer solution without adsorbed impurity (egg
It is white etc.), then magnetic bead is placed in the buffer solution of pH value 8.5, the DNA of purifying can enter in buffer solution.
Wherein, magnetic bead-DNA washing process need in magnetic bead-DNA purification system carry out, generally include 96 orifice plates and
Magnetic frame, 96 orifice plates have 96 reaction tubes for accommodating magnetic bead-DNA and cleaning solution；There are the magnetic frame 96 accommodating chambers to use
To carry reaction tube, each accommodating chamber includes at least one rectangular magnet, for providing magnetic field to magnetic bead-DNA.
As shown in figure 1, the radial cross-section of reaction tube 110 of prior art is circle, contacted in magnetic frame with reaction tube
Magnet 120 is permanent magnet, and surface is plane.As shown in Fig. 2 such reaction tube and the mode of magnet contact contact for point.Such as
Shown in Fig. 3, magnetic bead 130 is radially inside formed straight line during magnet adsorption in reaction tube wall, and magnetic bead is straight along this
Line is assembled, when being washed, and cleaning solution is only capable of washing the outer surface of magnetic bead accumulation regions, and the inside of magnetic bead accumulation regions can not be obtained
To washing, it is therefore desirable to which reaction tube is taken out into magnetic frame, disperseed magnetic bead, due to including miscellaneous inside magnetic bead accumulation regions
Matter, reaction tube is taken out after magnetic frame, and magnetic bead is still bonded together by impurity, it is therefore desirable to which placement is shaken in an oscillator, makes it
It is scattered, then washed, so operate repeatedly repeatedly, until magnetic bead-DNA is washed clean, is carrying out washing separation, operating numerous
It is trivial, in addition it is also necessary to add oscillator, add experimental cost.
Utility model content
Technical problem to be solved in the utility model is：A kind of magnetic frame is provided, it can improve nucleic acid purification mistake
The automaticity of journey.
In order to solve the above technical problems, the technical solution of the utility model is：
A kind of magnetic frame, it is used for Beads enrichment method seperated nuclear acid, and the magnetic frame includes support body and reaction tube；The frame
Body includes panel, the supporting plate being arranged at below panel and for support panel and the side plate of supporting plate, and the panel is set
There are some through holes, the reaction tube is placed in the supporting plate through the through hole；The reaction tube includes：Body, by pipe
Host cavity and be used to be used to accommodate magnetic bead and DNA mixed liquor with the extraneous mouth of pipe connected, the host cavity that body is limited,
The mouth of pipe is used for being put into or taking out for mixed liquor, and the mouth of pipe is vertically opened up, it is characterised in that：The magnetic frame
Also include electromagnet assembly, the electromagnet assembly is arranged on the side wall of the supporting plate or the support body, the body bag
At least one plane vertically extended is included, the plane reclines for electromagnet assembly, the electromagnet assembly is used to inhale
Assemble in magnetic bead to the plane of body in attached mixed liquor.
Further, some electromagnets include spaced first electromagnet and the second electromagnet, described first
Electromagnet and the second electromagnet are vertically arranged in the plane of reaction tube.
Further, first electromagnet connects electricity, and the second electromagnet does not connect electricity, and magnetic bead and DNA mixed liquor are first
Electromagnet near surface is assembled；First electromagnet is powered off, and magnetic bead and DNA mixed liquor are distributed in reaction tube；Second electromagnet
It is powered, magnetic bead is gathered in the reaction tube contacted with the second electromagnet.
Further, first electromagnet and the second electromagnet be in rectangular-shape, and the first electromagnet and second electricity
Magnet is equipped with the interface with the plane contact of reaction tube.
Further, first electromagnet is located at the surface of the second electromagnet, and the volume of first electromagnet is big
In the volume of second electromagnet.
Further, first electromagnet is located at the surface of the second electromagnet, the vertical side in the first electromagnet edge
Upward length be more than second electromagnet vertically on length.
Further, first electromagnet in transverse direction on width and second electromagnet in transverse direction on
Width it is identical.
Further, the reaction tube includes bottom of the tube, pipe shaft portion and spout part, and the bottom of the tube is small and push up in bottom
The big cone-shaped in end.
Further, the pipe shaft portion includes the cylinder cambered surface that is connected with plane, and the mouth of pipe is arranged at spout part, and to
Upper shed.
Further, the reaction tube has four sides, wherein four sides are plane, some electromagnets are also
Including spaced first electromagnet, the second electromagnet, the 3rd magnet and the 4th magnet, first electromagnet and the 3rd electricity
Magnet is vertically arranged on the one side of reaction tube, and second electromagnet and the 4th electromagnet are vertically arranged
On the another side of reaction tube.
Further, the magnet assemblies are arranged in array on the support body, and the reaction tube passes through the through hole quilt
It is arranged on the field regions that the magnet assemblies are surrounded.
Compared with prior art, the utility model at least has the advantages that：The use electromagnet of magnetic frame is provided
Magnetic field, can freely be controlled in the change in magnetic field, washing process by controlling electromagnet just to realize the aggregation of magnetic bead and divide
Dissipate, it is not necessary to which reaction tube is taken out into field regions, it is not necessary to which placement is shaken in an oscillator, improves whole nucleic acid purification mistake
The automaticity of journey, simplifies operation, improves operating efficiency.Magnetic frame provides magnetic field, elution process using double electromagnet
In only need to add a small amount of eluent and eluted, only connect second electromagnet and carry out magnetic bead absorption, reduce eluent consumption,
Improve DNA solution concentration.
Brief description of the drawings
Fig. 1 be prior art magnetic frame in reaction tube and magnet linear contact lay schematic diagram；
Fig. 2 is magnetic bead of the prior art by the coherent condition schematic diagram after magnet adsorption；
Fig. 3 is magnetic bead washing schematic diagram of the prior art；
Fig. 4 is reaction tube in the utility model embodiment and the schematic diagram of magnet contact；
Fig. 5 is magnetic bead in the utility model embodiment by the coherent condition schematic diagram after magnet adsorption；
Fig. 6 is the magnetic bead washing schematic diagram in the utility model embodiment；
Fig. 7 is the utility model embodiment magnetic frame overall structure side view；
Fig. 8 is the integrally-built top view of the utility model embodiment magnetic frame；
Fig. 9 is the schematic diagram of another embodiment of the utility model magnetic frame；
Figure 10 is the overall structure side view of another embodiment of the utility model magnetic frame.
Embodiment
The utility model is further illustrated with reference to the accompanying drawings and examples.
As shown in Fig. 4 to Fig. 8, to meet a kind of magnetic frame 100 of the utility model embodiment, it is used for Beads enrichment method
Seperated nuclear acid.
Magnetic frame 100 includes support body 200 and reaction tube 10；The support body 200 includes panel 201, is arranged at below panel
Supporting plate 202 and for support panel and the side plate of supporting plate 203, the panel 201 is provided with some through holes 20, described
Reaction tube 10 is placed in the supporting plate 202 through the through hole 20；The reaction tube 10 includes：Body 11, by body 11
The host cavity 12 of restriction and it is used to be used to accommodating mixing for magnetic bead 30 and DNA with the mouth of pipe 13 that connect of the external world, the host cavity 12
Liquid 40 is closed, the mouth of pipe 13 is used for being put into or taking out for mixed liquor 40, and the mouth of pipe 13 is vertically opened up, the magnetic force
Frame 100 also includes electromagnet assembly, and the electromagnet assembly is arranged on the supporting plate 202 or the side plate 203, the pipe
Body 11 includes at least one plane vertically extended, and the plane reclines for electromagnet assembly, the electromagnet assembly
Assemble for adsorbing the magnetic bead 30 in mixed liquor 40 to the plane of body 11.
The electromagnet assembly includes the spaced electromagnet 22 of first electromagnet 21 and second, first electromagnet
21 and second electromagnet 22 be vertically arranged in the plane 14 of reaction tube 10.
First electromagnet 21 connects electricity, and the second electromagnet 22 does not connect electricity, and magnetic bead 30 and DNA mixed liquor are in the first electromagnet 21
Near surface is assembled；First electromagnet power-off 21, magnetic bead 30 and DNA mixed liquor are distributed in reaction tube 10；Second electromagnet
22 are powered, and magnetic bead 30 is gathered in the reaction tube contacted with the second electromagnet 22.
First electromagnet 21 and the second electromagnet 22 are in rectangular-shape, and the first electromagnet 21 and the second electromagnet 22 are equal
Provided with the interface (non-label) with the plane contact of reaction tube.First electromagnet 21 be located at the second electromagnet 22 just on
Side, the volume of first electromagnet 21 is more than the volume of second electromagnet 22.First electromagnet 21 is along vertical side
Upward length be more than second electromagnet 22 vertically on length.First electromagnet 21 in transverse direction on
Width and second electromagnet 22 in transverse direction on width it is identical.
The reaction tube 10 include bottom of the tube 15, pipe shaft portion 16 and spout part 17, the bottom of the tube 15 in bottom it is small and
The big cone-shaped in top, the pipe shaft portion 16 includes the cylinder cambered surface 161 being connected with plane 14, and the mouth of pipe 13 is arranged at the mouth of pipe
Portion 17, and upward opening.
The angular range of the cross section circular arc of reaction tube 10 is 60 degree to 300 degree.
A kind of application method of magnetic frame, it comprises the following steps：
(1) reaction tube 10 is provided, the reaction tube 10 includes：Body 11, host cavity 12, the Yi Jiyong limited by body 11
With the mouth of pipe 13 connected with the external world, the host cavity 12 is used to accommodate magnetic bead 30 and DNA mixed liquor 40, and the mouth of pipe 13 is used for
Mixed liquor being put into or taking out, and the mouth of pipe 13 is vertically opened up, and the body 11 includes at least one vertically
The plane 14 of extension；
(2) the spaced electromagnet 22 of first electromagnet 21 and second, and the putting down to reaction tube 10 that reclined are provided
Face 14；
(3) first electromagnet 21 connect electricity, and the second electromagnet 22 does not connect electricity, and magnetic bead 30 and DNA mixed liquor 40 are in the first electricity
The near surface of magnet 21 is assembled, and once washing is completed, after cleaning solution is taken away；
(4) first electromagnet 21 are powered off, and magnetic bead 30 and DNA mixed liquor 40 are distributed in reaction tube 10, are adding washing
Liquid is washed, and is repeated several times；
(5) after magnetic bead 30 and DNA mixed liquors 40 are cleaned up, the first electromagnet 21 is powered off, by magnetic bead 30 and DNA mixed liquors
The bottom of reaction tube 10 is distributed to, a small amount of eluent is added and magnetic bead 30 and DNA mixed liquors is eluted, the second electromagnet 22
It is powered, magnetic bead 30 is gathered in the inwall of reaction tube 10 contacted with the second electromagnet 22, take eluent away, completes the He of magnetic bead 30
The separation of DNA molecular.
As shown in Fig. 9-Figure 10, to meet a kind of magnetic frame 300 of the utility model first embodiment, it has four sides
Face 30, wherein four sides 30 are plane, some electromagnets also include the spaced electricity of first electromagnet 31, second
Magnet 32, the 3rd magnet 33 and the 4th magnet 34, the electromagnet 33 of the first electromagnet 31 and the 3rd are vertically arranged in
On the one side of reaction tube, the electromagnet 34 of the second electromagnet 32 and the 4th is vertically arranged in the opposite side of reaction tube
On face.
Compared with prior art, the use electromagnet of the utility model magnetic frame provides magnetic field, can freely control magnetic field
Change, by controlling electromagnet just to realize the aggregation of magnetic bead and scattered in washing process, it is not necessary to which reaction tube is taken out
Field regions, it is not necessary to which placement is shaken in an oscillator, is improved the automaticity of whole nucleic acid purification process, is simplified
Operation, improves operating efficiency.Magnetic frame is provided using double electromagnet and only needs to add a small amount of eluent in magnetic field, elution process
Eluted, only connect the second electromagnet and carry out magnetic bead absorption, save eluent, reduce cost.
The technical process of magnetic frame is as follows：
1. sample is added, plus lysate lysed sample, discharge DNA, plus magnetic bead adsorption of DNA；
2. the first magnet power supply is connected, with the first magnet adsorption magnetic bead in the reaction tube of the first magnet, inhale
Walk waste liquid；
3. add rinsing liquid 1；
4. controlling the first magnet and the second magnet alternate communication power supply, and then magnetic bead is controlled close to the first magnet and second
It is returned on the reaction tube side wall of magnet for movement, plays the effect of similar concussion washing；
5. the first magnet adsorption magnetic bead, siphons away waste liquid；
6. rinse-added liquid 2, the first magnet and the second magnet alternating sorbent magnetic bead；
7. the 3rd magnet adsorption magnetic bead, siphons away waste liquid, dry；
8. add on eluent (only need to add a small amount of eluent, be added to adsorption section and the junction of connecting portion) elution magnetic bead
DNA, the 3rd magnet and the 4th magnet alternating sorbent magnetic bead play the effect of similar concussion washing, make the DNA on magnetic bead abundant
Elute；
9. the magnetic bead after the elution of the 3rd magnet adsorption, siphons away DNA solution.
The utility model is not limited to above-mentioned specific embodiment, and one of ordinary skill in the art visualizes from above-mentioned
Hair, without performing creative labour, made a variety of conversion are all fallen within protection domain of the present utility model.
Claims (10)
1. a kind of magnetic frame, it is used for Beads enrichment method seperated nuclear acid, and the magnetic frame includes support body and reaction tube；The support body
Including panel, the supporting plate being arranged at below panel and for support panel and the side plate of supporting plate, the panel is provided with
Some through holes, the reaction tube is placed in the supporting plate through the through hole；The reaction tube includes：Body, by body
The host cavity of restriction and be used to the mouth of pipe that connect of the external world, the host cavity is for accommodating magnetic bead and DNA mixed liquor, institute
Stating the mouth of pipe is used for being put into or taking out for mixed liquor, and the mouth of pipe is vertically opened up, it is characterised in that：The magnetic frame is also
Including electromagnet assembly, the electromagnet assembly is arranged on the side wall of the supporting plate or the support body, and the body includes
At least one plane vertically extended, the plane reclines for electromagnet assembly, and the electromagnet assembly is used to adsorb
Assemble in magnetic bead to the plane of body in mixed liquor.
2. the magnetic frame as described in claim 1, it is characterised in that：The electromagnet assembly includes the spaced first electricity
Magnet and the second electromagnet, first electromagnet and the second electromagnet are vertically arranged in the plane of reaction tube.
3. the magnetic frame as described in claim 2, it is characterised in that：First electromagnet connects electricity, and the second electromagnet does not connect
Electricity, magnetic bead and DNA mixed liquor are assembled in the first electromagnet near surface；First electromagnet is powered off, magnetic bead and DNA mixed liquor
It is distributed in reaction tube；Second electromagnet is powered, and magnetic bead is gathered in the reaction tube contacted with the second electromagnet.
4. the magnetic frame as described in claim 2, it is characterised in that：First electromagnet and the second electromagnet are in rectangular
Body shape, and the first electromagnet and the second electromagnet are equipped with the interface with the plane contact of reaction tube.
5. the magnetic frame as described in claim 2, it is characterised in that：First electromagnet is located at the upper of the second electromagnet
Side, the volume of first electromagnet is more than the volume of second electromagnet.
6. the magnetic frame as described in claim 2, it is characterised in that：First electromagnet is located at the upper of the second electromagnet
Side, first electromagnet vertically on length be more than second electromagnet vertically on length.
7. the magnetic frame as described in claim 1, it is characterised in that：The reaction tube includes bottom of the tube, pipe shaft portion and pipe
Oral area, one or more of the bottom of the tube shape in " V " shape or " u "-shaped.
8. the magnetic frame as described in claim 7, it is characterised in that：The pipe shaft portion includes the cylinder arc being connected with plane
Face, the mouth of pipe is arranged at spout part, and upward opening.
9. the magnetic frame as described in claim 1, it is characterised in that：The reaction tube has four sides, wherein four sides
Face is plane, and some electromagnets also include spaced first electromagnet, the second electromagnet, the 3rd magnet and the 4th
Magnet, first electromagnet and the 3rd electromagnet are vertically arranged on the one side of reaction tube, second electromagnetism
Iron and the 4th electromagnet are vertically arranged on the another side of reaction tube.
10. the magnetic frame as described in claim 1, it is characterised in that：The magnet assemblies are arranged in array in the support body
On, the reaction tube is arranged on the field regions that the magnet assemblies are surrounded through the through hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621316734.1U CN206345848U (en) | 2016-12-02 | 2016-12-02 | Magnetic frame |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621316734.1U CN206345848U (en) | 2016-12-02 | 2016-12-02 | Magnetic frame |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206345848U true CN206345848U (en) | 2017-07-21 |
Family
ID=59318550
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621316734.1U Expired - Fee Related CN206345848U (en) | 2016-12-02 | 2016-12-02 | Magnetic frame |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206345848U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020192090A1 (en) * | 2019-03-27 | 2020-10-01 | 苏州长光华医生物医学工程有限公司 | Kit storage rack preventing magnetic particle deposition |
| CN117757786A (en) * | 2024-02-22 | 2024-03-26 | 山东艾克韦生物技术有限公司 | nucleic acid purification auxiliary device and nucleic acid purification experiment method |
-
2016
- 2016-12-02 CN CN201621316734.1U patent/CN206345848U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020192090A1 (en) * | 2019-03-27 | 2020-10-01 | 苏州长光华医生物医学工程有限公司 | Kit storage rack preventing magnetic particle deposition |
| CN117757786A (en) * | 2024-02-22 | 2024-03-26 | 山东艾克韦生物技术有限公司 | nucleic acid purification auxiliary device and nucleic acid purification experiment method |
| CN117757786B (en) * | 2024-02-22 | 2024-05-10 | 山东艾克韦生物技术有限公司 | Nucleic acid purification auxiliary device and nucleic acid purification experiment method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170721 Termination date: 20191202 |