CN213668951U - Mouse cell suspension culture magnetic stirring device - Google Patents
Mouse cell suspension culture magnetic stirring device Download PDFInfo
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- CN213668951U CN213668951U CN202022617180.1U CN202022617180U CN213668951U CN 213668951 U CN213668951 U CN 213668951U CN 202022617180 U CN202022617180 U CN 202022617180U CN 213668951 U CN213668951 U CN 213668951U
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- kettle body
- stirring
- suspension culture
- motor
- stirring device
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Abstract
The utility model discloses a magnetic stirring device for suspension culture of mouse cells, which is provided with a stirring motor, a driving rod and a fixed stirring paddle are driven to rotate cell sap in the middle part and the top of a stirring kettle body, and a driving motor and a magnetic box are arranged to drive a suspension rotor to further stir the cell sap at the bottom of the stirring kettle body, so that the cell sap in the stirring kettle body is more uniformly stirred; the sensing probe and the controller are convenient for monitoring and displaying the change data of the temperature, the pressure, the cell sap stirring and the like in the kettle body in real time; the linear guide rail motor, the stirring motor, the driving motor, the storage battery and the like are convenient for realizing automatic control under the electrical connection of the controller and the processor; the utility model discloses a mouse cell suspension culture magnetic stirring device can realize good stirring effect, improves cell culture's effect.
Description
Technical Field
The utility model belongs to the technical field of agitating unit, in particular to magnetic stirring device is cultivateed in mouse cell suspension.
Background
When mouse Insulin (INS) measurement is carried out in a laboratory, liquid samples such as mouse serum, blood plasma and the like need to be subjected to operations such as supernatant, centrifugation, culture and the like, wherein suspension culture refers to a tissue culture system for culturing single cells and small cell clusters in a liquid culture medium which is constantly stirred or shaken, and is a culture mode of anchorage-independent cells.
The magnetic stirrer is a laboratory instrument for mixing liquid, is mainly used for stirring or simultaneously heating and stirring low-viscosity liquid or solid-liquid mixture, and has the basic principle that magnetic stirrers placed in a container are pushed by a magnetic field to perform circumferential operation by utilizing the principle that like poles repel and opposite poles attract of the magnetic field, so that the aim of stirring the liquid is fulfilled.
Most animal cells suspension culture magnetic stirring device sold on the market at present is optimized inadequately in structure, reasonable inadequately in design, single function, and degree of automation is low, can't satisfy user's demand. Therefore, we improve the technology and propose a magnetic stirring device for mouse cell suspension culture.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a mouse cell suspension culture magnetic stirring device can realize good stirring effect, improves cell culture's effect.
In order to achieve the above purpose, the solution of the present invention is:
a magnetic stirring device for suspension culture of mouse cells comprises a base, wherein a magnetic box and a support column are installed on the base, and a driving motor is arranged inside the base;
a rotating shaft is vertically arranged in the magnetic box, the bottom end of the rotating shaft is connected with an output shaft of a driving motor, a rotating disc is arranged at the top end of the rotating shaft, and an electromagnet is fixed at the bottom of the rotating disc;
a lantern ring is sleeved on the supporting column in a penetrating manner, and a ring opening is further formed in the lantern ring and used for clamping and fixing the kettle body; the heating pipe is arranged on the inner wall of the kettle body, the pit is arranged at the bottom of the inner wall of the kettle body, the suspension rotor is arranged in the pit, the suspension rotor and the electromagnet are in magnetic action, and when the driving motor drives the rotary disc to rotate, the electromagnet drives the suspension rotor to act, so that liquid at the bottom of the kettle body is stirred; the kettle body is also communicated with the outside through a discharge pipe;
the top of the kettle body is provided with a kettle cover, the top surface of the kettle cover is provided with a stirring motor, the bottom surface of the kettle cover is fixedly provided with a transmission rod positioned in the kettle body, the top end of the transmission rod is connected with an output shaft of the stirring motor, and the bottom end of the transmission rod is connected with and fixed with a stirring paddle;
the support rod is also provided with a linear guide motor, an output shaft of the linear guide motor is connected with one end of a connecting rod, and the other end of the connecting rod is connected with a stirring motor.
A plurality of groups of heating pipes are embedded in the inner wall of the kettle body at equal intervals.
The bottom of above-mentioned base is provided with shock attenuation callus on the sole.
The side of the base is provided with a heat dissipation port.
The side wall of the lantern ring which is sleeved on the supporting column is also provided with a fastening knob.
The edge of the kettle cover contacting with the kettle body is provided with a plurality of groups of sealing rubber rings to realize the sealing connection with the kettle body.
The kettle is internally provided with a sensing probe, a temperature sensor, a pressure sensor and a sound wave detection sensor, and the base is internally provided with a processor for receiving the sensing data of each sensor and transmitting the sensing data to a server.
The sensing probes are provided with a plurality of groups and are fixed inside the fixed stirring paddle and on the inner wall of the kettle body.
After the scheme is adopted, the utility model discloses compare prior art's beneficial effect is:
(1) the cell liquid stirring device drives the transmission rod and the fixed stirring paddle to rotate the cell liquid in the middle and at the top of the stirring kettle body by the stirring motor, and drives the suspension rotor to further stir the cell liquid at the bottom of the stirring kettle body by the driving motor and the magnetic box, thereby more uniformly stirring the cell liquid in the stirring kettle body;
(2) the utility model is provided with the sensing probe and the controller, which is convenient for real-time monitoring and displaying the internal temperature, pressure, cell sap stirring and other change data of the kettle, thereby facilitating the users to know the running condition of the device, and is provided with the processor and the server signal connection, thereby facilitating the terminal to store the device data, increasing the device data protection and being convenient for reading;
(3) the utility model discloses a be provided with linear guide motor, agitator motor, driving motor and battery etc to be convenient for realize the automation under controller and treater electric connection and control.
Drawings
FIG. 1 is an overall structure of the present invention;
FIG. 2 is a cross-sectional view of the inside of the middle kettle body of the present invention;
fig. 3 is an internal cross-sectional view of the base of the present invention.
Reference numerals:
1. a base; 2. a stirring body;
3. a controller; 4. a shock-absorbing foot pad;
5. a magnetic box; 6. a support pillar; 7. a chute;
8. an interface module; 9. fastening a knob;
10. a linear guide motor; 11. a connecting rod;
12. a stirring motor; 13. a kettle cover; 14. a collar;
15. a kettle body; 16. heating a tube; 17. fixing a stirring paddle;
18. a sensing probe; 19. a suspension rotor; 20. a discharge pipe;
21. a transmission rod; 22. a processor; 23. a drive motor;
24. a storage battery; 25. an electromagnet; 26. a rotating shaft; 27. a turntable.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary and intended to be used for explaining the present invention, and should not be construed as limiting the present invention.
As shown in fig. 1 to 3, the present invention provides a magnetic stirring apparatus for suspension culture of mouse cells, which comprises a base 1 and a stirring body 2, which are introduced below.
The controller 3 is fixedly installed on one side of the top end of the base 1, the magnetic box 5 is fixedly installed in the center of the top end of the base 1, the electromagnet 25, the rotating shaft 26 and the rotating disc 27 are arranged in the magnetic box 5, the supporting column 6 is fixedly installed on the other side of the top end of the base 1, and the processor 22, the driving motor 23 and the storage battery 24 are fixedly installed in the base 1 from one side to the other side in sequence;
the stirring main body 2 is provided with a support column 6, a linear guide rail motor 10, a kettle body 15 and a kettle cover 13, a lantern ring 14 and an interface module 8 are installed on the support column 6, a stirring motor 12, a transmission rod 21, a fixed stirring paddle 17 and a sensing probe 18 are installed on the kettle cover 13, and a heating pipe 16, a suspension rotor 19 and a discharge pipe 20 are installed on the kettle body 15.
As a preferred embodiment of the utility model, as shown in fig. 1, stirring main part 2 passes through support column 6 fixed mounting at base 1's top opposite side, and base 1's bottom four corners is provided with shock attenuation callus on the sole 4, and base 1 is rectangle structural design and bottom center both sides are provided with the thermovent.
As a preferred embodiment of the present invention, as shown in fig. 1, sliding grooves 7 are provided on both sides of the surface of the supporting column 6, and a transmission gear set is provided inside the sliding grooves 7 and is in transmission connection with a driving motor 23; the lantern ring 14 is in 8-shaped structural design, the openings of the two ends of the lantern ring are larger and smaller, and the inner wall of the small opening at the other end of the lantern ring 14 is provided with a rotating tooth sleeve buckle which is arranged on the surface of the supporting column 6 and is in transmission connection with the sliding chute 7.
As a preferred embodiment of the utility model, as shown in fig. 1, the top at the cauldron body 15 is installed to big ring mouth one side cover knot of the lantern ring 14, and the little ring mouth outer wall back of the lantern ring 14 is provided with fastening knob 9 and support column 6 fixed connection, and interface module 8 is provided with secondary feed inlet, discharge gate and auxiliary material import, and interface module 8's inside is provided with a plurality of groups pipeline and extends to big ring mouth one side and the 15 intercommunications of cauldron body.
As a preferred embodiment of the present invention, as shown in fig. 1, linear guide motor 10 is fixedly mounted on the top of support column 6, sliding-mounted is provided with connecting rod 11 in the middle of linear guide motor 10, the other end of connecting rod 11 is fixedly connected with agitator motor 12, agitator motor 12 is provided with protection frame and is fixedly mounted at the top center of kettle cover 13, and the bottom edge of kettle cover 13 is provided with the top sealing connection of multiunit sealing rubber ring and kettle body 15.
As a preferred embodiment of the present invention, as shown in fig. 2, the transmission rod 21 is installed in the center of the kettle body 15 in an extending manner, and the top end thereof penetrates through the kettle cover 13 and is connected with the stirring motor 12 in a transmission manner; the fixed stirring paddles 17 are arranged in two groups, are fixedly arranged in the middle and at the bottom of the transmission rod 21, and are provided with a plurality of groups of paddles; the sensing probe 18 comprises a temperature sensor, a pressure sensor, a sound wave detection sensor and the like, and the sensing probe 18 is provided with a plurality of groups and fixedly embedded and installed inside the fixed stirring paddle 17 and on the inner wall of the kettle body 15.
As a preferred embodiment of the present invention, as shown in fig. 2, a pit is provided at the bottom of the inner wall of the kettle body 15, and the suspension rotor 19 is fixedly installed inside the pit of the kettle body 15; the heating pipe 16 is provided with a plurality of groups and is equidistantly embedded and installed in the inner wall of the kettle body 15, the bottom of the discharging pipe 20 is in an arc-shaped structural design, and is fixedly installed on the inner wall of the kettle body 15 and is butted with the discharging port on the lantern ring 14.
As a preferred embodiment of the present invention, as shown in fig. 3, the storage battery 24 is fixedly installed at the other side inside the base 1, and the driving motor 23 is fixedly installed at the center inside the base 1; the turntable 27 is clamped and installed on the top of the inner wall of the magnetic box 5, the electromagnets 25 are provided with two sides and are fixedly installed on two sides of the bottom end of the turntable 27, and the rotating shaft 26 is fixedly installed in the center of the bottom end of the turntable 27 and is in transmission connection with the output end of the driving motor 23.
As a preferred embodiment of the present invention, as shown in fig. 3, the controller 3 is an inclination angle and is fixedly installed on one side of the top end of the base 1, the processor 22 is fixedly installed on one side of the inside of the base 1 and electrically connected to the controller 3, the inside of the processor 22 is provided with a wireless module and a server signal connection, and the controller 3 is electrically connected to the electrical appliances on the base 1 and the stirring main body 2 through the processor 22.
The utility model discloses a theory of operation is: when the device is used, the device is started through the controller 3, the cell sap is stored, collected and auxiliary materials are butted with the device through the interface module, the linear guide rail motor 10, the stirring motor 12, the driving motor 23, the storage battery 24 and the like are arranged, so that the automatic operation and control can be conveniently realized under the wire connection of the controller and the processor, the stirring motor 12 is arranged to drive the transmission rod 21 and the fixed stirring paddle 17 to rotate the cell sap in the middle and the top of the stirring kettle body 15, the cell sap at the bottom of the stirring kettle body 15 is further stirred through the driving motor 23 and the magnetic box 5, so that the cell sap in the stirring kettle body 15 is more uniform, the sensing probe 18 and the controller 3 are arranged to conveniently monitor and display the change data of the temperature, the pressure, the cell sap stirring and the like in the kettle body 15 in real time, so that a user can conveniently know the running condition of the device, and, thereby be convenient for terminal save device data, increase device data protection and be convenient for read, the utility model has the advantages of simple and reasonable structure, the modern design, easy operation to can carry out effectual buffering shock attenuation to the device, have higher practical value.
In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description of the present invention and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above should not be understood to necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples described in this specification can be combined and combined by those skilled in the art.
Although embodiments of the present invention have been shown and described, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art without departing from the scope of the present invention.
Claims (8)
1. The utility model provides a mouse cell suspension culture magnetic stirring device which characterized in that: the magnetic box is characterized by comprising a base, wherein a magnetic box and a support column are arranged on the base, and a driving motor is arranged in the base;
a rotating shaft is vertically arranged in the magnetic box, the bottom end of the rotating shaft is connected with an output shaft of a driving motor, a rotating disc is arranged at the top end of the rotating shaft, and an electromagnet is fixed at the bottom of the rotating disc;
a lantern ring is sleeved on the supporting column in a penetrating manner, and a ring opening is further formed in the lantern ring and used for clamping and fixing the kettle body; the heating pipe is arranged on the inner wall of the kettle body, the pit is arranged at the bottom of the inner wall of the kettle body, the suspension rotor is arranged in the pit, the suspension rotor and the electromagnet are in magnetic action, and when the driving motor drives the rotary disc to rotate, the electromagnet drives the suspension rotor to act, so that liquid at the bottom of the kettle body is stirred; the kettle body is also communicated with the outside through a discharge pipe;
the top of the kettle body is provided with a kettle cover, the top surface of the kettle cover is provided with a stirring motor, the bottom surface of the kettle cover is fixedly provided with a transmission rod positioned in the kettle body, the top end of the transmission rod is connected with an output shaft of the stirring motor, and the bottom end of the transmission rod is connected with and fixed with a stirring paddle;
still be equipped with the linear guide motor on the support column, the output shaft of linear guide motor is connected with the one end of connecting rod, and the other end and the agitator motor of connecting rod are connected.
2. The magnetic stirring device for mouse cell suspension culture according to claim 1, wherein: and a plurality of groups of heating pipes are embedded in the inner wall of the kettle body at equal intervals.
3. The magnetic stirring device for mouse cell suspension culture according to claim 1, wherein: the bottom of base is provided with shock attenuation callus on the sole.
4. The magnetic stirring device for mouse cell suspension culture according to claim 1, wherein: the side of the base is provided with a heat dissipation port.
5. The magnetic stirring device for mouse cell suspension culture according to claim 1, wherein: the lantern ring is also provided with a fastening knob at the side wall of the penetrating and sleeving support column.
6. The magnetic stirring device for mouse cell suspension culture according to claim 1, wherein: the kettle cover is provided with a plurality of groups of sealing rubber rings at the edge contacting with the kettle body, so that the kettle cover is hermetically connected with the kettle body.
7. The magnetic stirring device for mouse cell suspension culture according to claim 1, wherein: the internal sensing probe that is equipped with of cauldron installs temperature sensor, pressure sensor and sound wave and detects the sensor, and is equipped with the treater in base inside for receive the sensing data of each sensor and convey to the server.
8. The magnetic stirring device for mouse cell suspension culture as claimed in claim 7, wherein: the sensing probes are provided with a plurality of groups and fixed inside the fixed stirring paddle and on the inner wall of the kettle body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022617180.1U CN213668951U (en) | 2020-11-12 | 2020-11-12 | Mouse cell suspension culture magnetic stirring device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022617180.1U CN213668951U (en) | 2020-11-12 | 2020-11-12 | Mouse cell suspension culture magnetic stirring device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213668951U true CN213668951U (en) | 2021-07-13 |
Family
ID=76731600
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022617180.1U Active CN213668951U (en) | 2020-11-12 | 2020-11-12 | Mouse cell suspension culture magnetic stirring device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213668951U (en) |
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2020
- 2020-11-12 CN CN202022617180.1U patent/CN213668951U/en active Active
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