CN214654799U - Cell separation self-dissociation device - Google Patents
Cell separation self-dissociation device Download PDFInfo
- Publication number
- CN214654799U CN214654799U CN202022903367.8U CN202022903367U CN214654799U CN 214654799 U CN214654799 U CN 214654799U CN 202022903367 U CN202022903367 U CN 202022903367U CN 214654799 U CN214654799 U CN 214654799U
- Authority
- CN
- China
- Prior art keywords
- separation
- cell
- column
- peristaltic pump
- streptavidin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Peptides Or Proteins (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a cell separation is from dissociation device, including sample room, peristaltic pump and detached column, the peristaltic pump is installed in connection of sample room one side, and the peristaltic pump other end is connected and is installed the detached column to the peristaltic pump is located the detached column upper end, the detached column bottom is connected and is installed and receive the chamber, and receives intracavity portion and be provided with the receipt steamer tray, the inside separation microballon that is provided with of detached column. This cell separation is from dissociation device is through connecting streptavidin after the separation microsphere surface is modified with desulfurization biotin for can combine with the streptavidin on separation microsphere surface when the cell with biotinylation antibody mark flows through the knockout column, when biotin competitiveness combines streptavidin, alright come down with the cell elution, and through the knockout column of vertical setting and the inside separation microsphere of knockout column, elute the back to the cell, can collect the cell of separation, the follow-up application of the low reaches of being convenient for.
Description
Technical Field
The utility model relates to a bioengineering technical field specifically is a cell separation is from dissociation device.
Background
The cell is used as a basic constitution unit of human body and animal, is an important basic raw material of scientific research institutions and drug research institutions, almost all biological basic research and drug research and development need to use the cell, the product quality of the cell is directly related to the quality of the whole scientific research experiment and drug research, the cell often exists in tissue or blood, the precise research of a certain specific cell needs to separate and purify the specific cell from different types of tissue and blood, and the current cell separation has certain problems:
1. the currently common cell separation and purification method is a magnetic bead cell separation method (magnetic activated cell sorting), which combines cells with magnetic beads of labeled antibodies by using the principle of antigen-antibody combination, and then separates the magnetic beads in a magnetic field, but the product device used in the cell separation method is complex, a magnetic field generating device is needed, the product price is high, the separation of common cells and the separation device price are all ten thousand RMB, and the operation cost is too high;
2. in addition, the method of separating cells using magnetic beads is complicated in operation, requires the connection of antibodies, and also requires steps such as magnetic pole sorting and elution, which takes a long time and is inefficient.
Aiming at the problems, the novel design is carried out on the basis of the original cell separation.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a cell separation is from dissociation device to the product device that the magnetic bead cell separation method commonly used on the existing market that the solution above-mentioned background art provided used is complicated, and the product is expensive, and is comparatively loaded down with trivial details in the operation, needs to connect the antibody, still need carry out the magnetic pole and select separately and step such as elution, the problem of the time length of cost.
In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a cell separation is from dissociation device, includes sample room, peristaltic pump and separator, the peristaltic pump is installed in connection of sample room one side, and the peristaltic pump other end is connected and is installed the separator to the peristaltic pump is located the separator upper end, the separator bottom is connected and is installed and receive the chamber, and receives the intracavity portion and be provided with the receipt steamer tray, the inside separation microballon that is provided with of separator.
Preferably, the diameter of the separation column is 2cm, and the length of the separation column is 10 cm.
Compared with the prior art, the beneficial effects of the utility model are that: the cells are isolated from a dissociation device,
1. the surface of the separation microsphere is modified by desulfurized biotin and then is connected with streptavidin, so that cells marked by biotinylated antibodies can be combined with the streptavidin on the surface of the separation microsphere when flowing through the separation column, and the cells can be eluted when the biotin is competitively combined with the streptavidin;
2. through the inside separation microballon of the fractionating column and the vertical setting, elute the back to the cell, the cell can fall into the receiving intracavity of fractionating column bottom between, can collect the cell of accomplishing through receiving the steamer tray, the follow-up application of the low reaches of being convenient for.
Drawings
FIG. 1 is a schematic view of the overall front view structure of the present invention;
fig. 2 is a schematic view of the overall front view cross-sectional structure of the present invention.
In the figure: 1. a sample chamber; 2. a peristaltic pump; 3. a separation column; 4. a receiving cavity; 5. a receiving drawer; 6. the microspheres are isolated.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-2, the present invention provides a technical solution: the utility model provides a cell separation is from dissociation device, includes sample room 1, peristaltic pump 2 and separator 3, and 1 one side of sample room is connected and is installed peristaltic pump 2, and the 2 other ends of peristaltic pump connect and install separator 3 to peristaltic pump 2 is located the 3 upper ends of separator, and 3 bottoms of separator are connected and are installed and receive chamber 4, and receive 4 insides in chamber and be provided with and receive steamer tray 5, and 3 insides of separator are provided with separation microballon 6.
The diameter of the separation column 3 was 2cm, and the length of the separation column 3 was 10 cm.
The working principle is as follows: according to the fig. 1-2, when using the cell separation self-dissociation device, firstly, a worker fuses the cells to be separated with the biotinylated antibody, so that the cells are labeled with the biotinylated antibody, then the cells are placed in the sample chamber 1, the cells labeled with the biotinylated antibody are conveyed into the separation column 3 through the peristaltic pump 2, the cells labeled with the biotinylated antibody pass through the column and then contact with the separation microspheres 6 in the separation column 3, the surface of the separation microspheres 6 is modified with desthiobiotin and then is connected with streptavidin, the cells labeled with the biotinylated antibody pass through the column and then are combined with the streptavidin on the separation column 3, after using excessive free biotin flow through the separation column 3, the streptavidin can be competitively combined to cause the cells to be eluted, the eluted cells flow into the receiving drawer 5 in the receiving cavity 4, the cells in the receiving drawer 5 are collected by the operator for downstream use, and are not described in detail in this specification as being well known to those skilled in the art.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.
Claims (2)
1. A cell separation self-dissociation device comprises a sample chamber (1), a peristaltic pump (2) and a separation column (3), and is characterized in that: the device is characterized in that a peristaltic pump (2) is connected to one side of the sample chamber (1), a separation column (3) is connected to the other end of the peristaltic pump (2), the peristaltic pump (2) is located at the upper end of the separation column (3), a receiving cavity (4) is connected to the bottom of the separation column (3), a receiving drawer (5) is arranged inside the receiving cavity (4), and separation microspheres (6) are arranged inside the separation column (3).
2. A cell separation self-dissociation device according to claim 1, wherein: the diameter of the separation column (3) is 2cm, and the length of the separation column (3) is 10 cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022903367.8U CN214654799U (en) | 2020-12-04 | 2020-12-04 | Cell separation self-dissociation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022903367.8U CN214654799U (en) | 2020-12-04 | 2020-12-04 | Cell separation self-dissociation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214654799U true CN214654799U (en) | 2021-11-09 |
Family
ID=78498123
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022903367.8U Active CN214654799U (en) | 2020-12-04 | 2020-12-04 | Cell separation self-dissociation device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214654799U (en) |
-
2020
- 2020-12-04 CN CN202022903367.8U patent/CN214654799U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| GB1508844A (en) | Filter device for separating blood samples into fractions | |
| CN109364525B (en) | Mesenchymal exosome separation and extraction device and method | |
| González‐González et al. | Current strategies and challenges for the purification of stem cells | |
| CN214654799U (en) | Cell separation self-dissociation device | |
| GB873494A (en) | Method and means for centrifuging | |
| Lafon et al. | Use of a monoclonal antibody for quantitation of rabies vaccine glycoprotein by enzyme immunoassay | |
| CN110653012A (en) | Multistage microfluidic chip device for sorting, enriching and detecting circulating tumor cells | |
| CN203625357U (en) | Circulating tumor cell separation system | |
| Kumar et al. | Cell separation: a review | |
| CN103235124A (en) | Nitrocellulose-membrane-based chip for conveniently capturing cancer cells | |
| CN205653449U (en) | Simple and easy device with negative pressure method purification nucleic acid | |
| CN205603599U (en) | Magnetic shelf with elastic bulge | |
| Popovic | Routine and novel methods for isolation of extracellular vesicles | |
| CN207287738U (en) | Multifunctional magnetic-force frame | |
| CN201020402Y (en) | Detachable utility type magnetic separating rack | |
| CN106092679A (en) | Liquid basal cell natural subsidence pelletizer | |
| CN202400998U (en) | Protein electrophoresis separation device | |
| CN207540863U (en) | A kind of fast filtering and the sample cell of separation | |
| Revillard et al. | Clinical significance of β2 microglobulin determination | |
| CN104231037A (en) | Rapid protein purification combination chromatographic column, kit and preparation method | |
| CN210103801U (en) | Antibody separation device | |
| CN209317893U (en) | A kind of controllable magnetic separator | |
| CN103739692A (en) | Method for preparing beta2 microglobulin crude product | |
| CN210488991U (en) | Series-parallel combined experimental device for natural science experiment | |
| CN210765087U (en) | Purified antibody collection device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |