CN109259018A - The Preparation method and use of extractive of general flavone with health care function in a kind of bunge pricklyash leaf - Google Patents
The Preparation method and use of extractive of general flavone with health care function in a kind of bunge pricklyash leaf Download PDFInfo
- Publication number
- CN109259018A CN109259018A CN201811244878.4A CN201811244878A CN109259018A CN 109259018 A CN109259018 A CN 109259018A CN 201811244878 A CN201811244878 A CN 201811244878A CN 109259018 A CN109259018 A CN 109259018A
- Authority
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- China
- Prior art keywords
- general flavone
- bunge pricklyash
- pricklyash leaf
- extractive
- health care
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- Pending
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- 241000949456 Zanthoxylum Species 0.000 title claims abstract description 47
- 235000007650 Aralia spinosa Nutrition 0.000 title claims abstract description 46
- 241000612118 Samolus valerandi Species 0.000 title claims abstract description 46
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 title claims abstract description 42
- 229930003944 flavone Natural products 0.000 title claims abstract description 42
- 150000002212 flavone derivatives Chemical class 0.000 title claims abstract description 42
- 235000011949 flavones Nutrition 0.000 title claims abstract description 42
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 title claims abstract description 42
- 230000036541 health Effects 0.000 title claims abstract description 33
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 235000013361 beverage Nutrition 0.000 claims abstract description 13
- 230000003712 anti-aging effect Effects 0.000 claims abstract description 4
- 239000003963 antioxidant agent Substances 0.000 claims abstract description 4
- 230000003078 antioxidant effect Effects 0.000 claims abstract description 4
- 235000006708 antioxidants Nutrition 0.000 claims abstract description 4
- 230000002265 prevention Effects 0.000 claims abstract description 4
- 208000024172 Cardiovascular disease Diseases 0.000 claims abstract description 3
- 208000026106 cerebrovascular disease Diseases 0.000 claims abstract description 3
- 230000002526 effect on cardiovascular system Effects 0.000 claims abstract description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 123
- 235000019441 ethanol Nutrition 0.000 claims description 56
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 40
- 239000000284 extract Substances 0.000 claims description 30
- 244000089698 Zanthoxylum simulans Species 0.000 claims description 23
- 238000000605 extraction Methods 0.000 claims description 22
- 239000000287 crude extract Substances 0.000 claims description 17
- 238000010828 elution Methods 0.000 claims description 16
- 239000011259 mixed solution Substances 0.000 claims description 16
- 239000000047 product Substances 0.000 claims description 13
- 238000000265 homogenisation Methods 0.000 claims description 12
- 239000002904 solvent Substances 0.000 claims description 12
- 229930006000 Sucrose Natural products 0.000 claims description 8
- 238000011049 filling Methods 0.000 claims description 8
- 239000011347 resin Substances 0.000 claims description 8
- 229920005989 resin Polymers 0.000 claims description 8
- 239000000243 solution Substances 0.000 claims description 8
- 239000005720 sucrose Substances 0.000 claims description 8
- 235000003599 food sweetener Nutrition 0.000 claims description 7
- 239000003765 sweetening agent Substances 0.000 claims description 7
- 238000010521 absorption reaction Methods 0.000 claims description 6
- MKJXYGKVIBWPFZ-UHFFFAOYSA-L calcium lactate Chemical compound [Ca+2].CC(O)C([O-])=O.CC(O)C([O-])=O MKJXYGKVIBWPFZ-UHFFFAOYSA-L 0.000 claims description 6
- 229960002401 calcium lactate Drugs 0.000 claims description 6
- 235000011086 calcium lactate Nutrition 0.000 claims description 6
- 239000001527 calcium lactate Substances 0.000 claims description 6
- 239000003480 eluent Substances 0.000 claims description 6
- 239000000706 filtrate Substances 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 229930003935 flavonoid Natural products 0.000 claims description 6
- 150000002215 flavonoids Chemical class 0.000 claims description 6
- 235000017173 flavonoids Nutrition 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 239000011148 porous material Substances 0.000 claims description 6
- 230000001376 precipitating effect Effects 0.000 claims description 6
- 238000001179 sorption measurement Methods 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- -1 sucrose ester Chemical class 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 6
- 238000002137 ultrasound extraction Methods 0.000 claims description 6
- 229920001285 xanthan gum Polymers 0.000 claims description 6
- 229940082509 xanthan gum Drugs 0.000 claims description 6
- 235000010493 xanthan gum Nutrition 0.000 claims description 6
- 239000000230 xanthan gum Substances 0.000 claims description 6
- 239000008236 heating water Substances 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
- 238000010992 reflux Methods 0.000 claims description 5
- 238000002791 soaking Methods 0.000 claims description 5
- 239000004615 ingredient Substances 0.000 claims description 4
- 230000002708 enhancing effect Effects 0.000 claims description 3
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 2
- 235000008534 Capsicum annuum var annuum Nutrition 0.000 claims description 2
- 240000008384 Capsicum annuum var. annuum Species 0.000 claims description 2
- 229930091371 Fructose Natural products 0.000 claims description 2
- 239000005715 Fructose Substances 0.000 claims description 2
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 2
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 2
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 2
- 239000004952 Polyamide Substances 0.000 claims description 2
- 229920002472 Starch Polymers 0.000 claims description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 2
- 230000002745 absorbent Effects 0.000 claims description 2
- 239000002250 absorbent Substances 0.000 claims description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 2
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 claims description 2
- 239000008103 glucose Substances 0.000 claims description 2
- 230000036039 immunity Effects 0.000 claims description 2
- 239000008101 lactose Substances 0.000 claims description 2
- 229920002647 polyamide Polymers 0.000 claims description 2
- 239000008107 starch Substances 0.000 claims description 2
- 235000019698 starch Nutrition 0.000 claims description 2
- 239000006188 syrup Substances 0.000 claims 1
- 235000020357 syrup Nutrition 0.000 claims 1
- 230000006870 function Effects 0.000 abstract description 12
- 230000036737 immune function Effects 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 239000002699 waste material Substances 0.000 abstract description 3
- 239000006227 byproduct Substances 0.000 abstract description 2
- 238000003912 environmental pollution Methods 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract description 2
- 241001079064 Zanthoxylum schinifolium Species 0.000 abstract 1
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 6
- 235000013305 food Nutrition 0.000 description 5
- 229940079593 drug Drugs 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 235000013402 health food Nutrition 0.000 description 4
- 230000002218 hypoglycaemic effect Effects 0.000 description 4
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 3
- 229930003268 Vitamin C Natural products 0.000 description 3
- 235000019154 vitamin C Nutrition 0.000 description 3
- 239000011718 vitamin C Substances 0.000 description 3
- 235000005911 diet Nutrition 0.000 description 2
- 230000037213 diet Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- UFLHIIWVXFIJGU-ARJAWSKDSA-N (Z)-hex-3-en-1-ol Chemical compound CC\C=C/CCO UFLHIIWVXFIJGU-ARJAWSKDSA-N 0.000 description 1
- 241000244203 Caenorhabditis elegans Species 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 206010016326 Feeling cold Diseases 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 241001093501 Rutaceae Species 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- 230000000202 analgesic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000006837 decompression Effects 0.000 description 1
- 230000035613 defoliation Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000027939 micturition Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000008447 perception Effects 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
- 208000019553 vascular disease Diseases 0.000 description 1
- 230000008673 vomiting Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/42—Preservation of non-alcoholic beverages
- A23L2/46—Preservation of non-alcoholic beverages by heating
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
- A23L2/60—Sweeteners
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/72—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by filtration
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses a kind of Preparation method and uses of extractive of general flavone with health care function in bunge pricklyash leaf, the present invention prepares extractive of general flavone as raw material using the byproduct bunge pricklyash leaf in Chinese prickly ash production process cheap and easy to get, its is easy to operate, it is easy to use, and without the dregs of a decoction, and avoid environmental pollution caused by waste；Health beverages of the invention, which have, to be enhanced and adjusting immune function of human body, anti-oxidant, anti-aging are improved looks, and eliminates fatigue, and functions, the application prospect such as prevention cardiovascular and cerebrovascular disease are good.
Description
Technical field
The invention belongs to technical field of health care, and in particular to general flavone with health care function mentions in a kind of bunge pricklyash leaf
Take the Preparation method and use of object.
Background technique
Chinese prickly ash is Rutaceae, and Zanthoxylum defoliation small arbor, bunge pricklyash leaf is the leaf of Chinese pricklyash, and Chinese prickly ash heat has analgesic, kills
Worm effect, can prevent and treat the diseases such as coldness and pain in the epigastrium, vomiting and diarrhoea, roundworm disease, oedema and difficult urination, bunge pricklyash leaf also have Chinese prickly ash performance and
Taste.Its rachis often has very narrow winged petiole, and leaf margin has checking tooth, and slot has oil drop.For blade back by pubescence, blade back is dry bronzing
Speckle.It is distributed North gets THE SOUTH OF NORTHEAST CHINA, south to Five Ridges north slope, the southeast to Jiangsu, Along Zhejiang Coast area, southwest to the Tibet southeast
Portion；Taiwan, Hainan and Guangdong do not produce.It is more common in Plain to the higher mountainous region of height above sea level, 2500 meters of height above sea level of hillside fields also has in Qinghai
Plantation, it is drought-enduring, sunlight is liked, many places can all plant.
With the improvement of economic situation, the enhancing of improvement of living standard and health perception, people in terms of diet not
It only has enough, eat, but also diet is required to meet nutrition requirement, be beneficial to keep fit and healthy.Thus, in addition to delicious food
Outside, miscellaneous health beverages also just come into being, wherein herb biology health beverages are effect health beverages outstanding
Product are the first choices of our health cares.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of extractive of general flavone with health care function in bunge pricklyash leaf and
Purposes.
In order to meet above-mentioned purpose, the technical scheme adopted by the invention is as follows:
A kind of preparation method of extractive of general flavone with health care function in bunge pricklyash leaf, it is characterised in that the preparation method packet
Include following steps:
(1) bunge pricklyash leaf is cleaned, is dried in the shade, then crushed, smashing fineness is 0.1-5 mm；
(2) by smashed bunge pricklyash leaf Extraction solvent by room temperature cold soaking, be heated to reflux, leak and one of ultrasonic extraction
Extracting method extracts, and the mass ratio of the bunge pricklyash leaf and Extraction solvent is 1:3-20, extracts 1-4 times, has extracted filtering, closes
And filtrate, heating water bath to 40-70 DEG C of vacuum-concentrcted, dry on a rotary evaporator, obtains general flavone crude extract A；
(3) Chinese prickly ash leaf flavonoids crude extract A is sufficiently dissolved with water, the general flavone crude extract A and water quality ratio are 1:
5-20, then dissolved general flavone crude extract A is added in large pore resin absorption column, 24 h of standing adsorption divides in order later
Not Yong volume multiple be 3.5 times of pure water, 30% ethyl alcohol, 60% ethyl alcohol, 85% ethyl alcohol and ethanol elution, each section of eluent difference is dense
It is reduced to elution medicinal extract paste, the elution medicinal extract is Chinese prickly ash leaf total flavone extract with health care function, is transferred to burning
Cup in, labelled spare, each section is numbered respectively are as follows: A(pure water), B(30% ethyl alcohol), C(60% ethyl alcohol), D(85% ethyl alcohol), E
(ethyl alcohol).
Preferably, the Extraction solvent is the mixed solution for the ethanol/water that percentage by volume is 0-100%.
Preferably, in step (2), the room temperature cold soaking extraction needs bunge pricklyash leaf impregnating 3- in Extraction solvent every time
10 days, the heating temperature of the heating and refluxing extraction was 50-100 DEG C, is extracted 0.5-3.0 hours every time, and the leakage, which is extracted, to be needed
Bunge pricklyash leaf is impregnated to 6-24h in Extraction solvent, then is placed in leakage equipment, the Extracting temperature of the ultrasonic extraction is 30-60
DEG C, frequency 20-50Hz, power 70-1000W are extracted 0.5-2.0 hours every time.
Preferably, macroporous absorbent resin is HPD-600, X-5, AB-8, polyamide, HZ-841, D- in the step (3)
One of 101 and HPD-100 are a variety of.
Preferably, in a kind of bunge pricklyash leaf extractive of general flavone with health care function purposes, including prepare health beverages,
The method for preparing health beverages the following steps are included:
(1) ingredient: the elution medicinal extract of C portion obtained in the claims 1 is soluble in water, the mass ratio of the medicinal extract and water
For 1:50-200, it is dense to add sucrose ester, quality that sweetener, mass concentration that mass concentration is 5%-15% are 0.02-0.05%
It is raw to spend the calcium lactate for being 0.15-0.2%, the xanthan gum that mass concentration is 1.0-3.0% and the dimension that mass concentration is 0.01-0.03%
Plain C, B1, B2, P；
(2) mixed solution deployed in step (1) ingredient is placed in homogenizer, is 15-30 MPa, temperature in homogenization pressure
Homogeneous under the conditions of 45-60 DEG C makes each homogenization of composition, prevents from being layered, and guarantees the stability of product；
(3) filter: the mixed solution after homogenizing is centrifuged by the centrifuge of 6000-10000 r/min at room temperature
10-20 min discards precipitating, collects supernatant；
(4) it sterilizes filling: by filtered clear solution in 120-130 DEG C of progress instantaneous sterilizing 4-6 s, after being cooled to room temperature
It is filling to get product.
Preferably, in the step (1), sweetener is the sugar of glucose or fructose, sucrose, maltose, starch sugar, lactose
Slurry.
Preferably, the health beverages of the preparation have enhancing and adjust the function of human immunity.
Preferably, the health beverages of the preparation have anti-oxidant, anti-aging, cosmetology, eliminate fatigue and the prevention heart
The function of cranial vascular disease.
The invention has the benefit that
(1) present invention prepares extractive of general flavone as raw material using the byproduct bunge pricklyash leaf in Chinese prickly ash production process cheap and easy to get,
Its is easy to operate, easy to use, and without the dregs of a decoction, and avoids environmental pollution caused by waste, and bunge pricklyash leaf is turned waste into wealth,
Increase green pepper agriculture income, thrust zone economic development etc. has great importance；
(2) extractive of general flavone extracted in bunge pricklyash leaf in the present invention, which has, enhances and adjusts immune function of human body, anti-oxidant,
Anti-aging is improved looks, and fatigue is eliminated, and the functions such as prevention cardiovascular and cerebrovascular disease are significant in efficacy,；
(3) since bunge pricklyash leaf is largely much like with the function of Chinese prickly ash, many places can all eat bunge pricklyash leaf, so this
The health beverages of Chinese prickly ash leaf extract production in invention can be eaten for a long time, and can enhance and adjust the immune function of human body.
Specific embodiment
The present invention is described further below with reference to working principle:
Embodiment 1:
(1) 5.0 kg of bunge pricklyash leaf is cleaned, is dried in the shade, then crushed, smashing fineness is 0.1 mm；
(2) smashed bunge pricklyash leaf being subjected to heating and refluxing extraction with water, heating temperature is 80 DEG C, it extracts 2.0 hours every time,
Extraction time is 3 times, and the mass ratio of bunge pricklyash leaf and water is 1:3, has extracted filtering, merging filtrate, on a rotary evaporator water-bath
40 DEG C of vacuum-concentrcteds are heated to, it is dry, obtain general flavone crude extract A；
(3) Chinese prickly ash leaf flavonoids crude extract is sufficiently dissolved with water, crosses HPD-600 large pore resin absorption column.Respectively with water, thick
Extract all be added columns in after, standing adsorption for 24 hours, later in order respectively with the pure water of 3.5 times of BV, 30% ethyl alcohol, 60% ethyl alcohol,
85% ethyl alcohol and ethanol elution, each section of eluent is concentrated into thick paste respectively, is transferred in beaker, labelled, spare, and each section point
Do not number are as follows: A(pure water), B(30% ethyl alcohol), C(60% ethyl alcohol), D(85% ethyl alcohol), E(ethyl alcohol).
C portion elutes general flavone volume parts >=40% of Chinese prickly ash extractive of general flavone in medicinal extract, and concentration highest as has
There is the Chinese prickly ash leaf total flavone extract of the drug of hypoglycemic effect, health food or food.
(4) it takes C portion elution 90 g of medicinal extract to be dissolved in 4500 g water, the sweetener that 6g mass concentration is 5%, 6g matter is added
Measure the sucrose ester of concentration 0.02, the calcium lactate that 6g mass concentration is 0.15, the dimension of xanthan gum and 0.3g that 6g mass concentration is 1.0
Raw element C, B1, B2, P.
(5) deployed mixed solution is placed in homogenizer, is 20 MPa in homogenization pressure, under the conditions of 50 DEG C of temperature
Matter makes each homogenization of composition, prevents from being layered, and guarantees the stability of product.
(6) mixed solution to homogenize is centrifuged 10 min in the centrifuge of 6000 r/min of room temperature, discards precipitating, receives
Collect supernatant.
(7) by filtered clear solution in 120 DEG C of progress 4 s of instantaneous sterilizing, be cooled to room temperature it is rear it is filling up at
Product.
Embodiment 2:
(1) 5.0 kg of bunge pricklyash leaf is cleaned, is dried in the shade, then crushed, smashing fineness is 2.5 mm；
(2) smashed bunge pricklyash leaf room temperature cold soaking is carried out with percentage by volume for the mixed solution of 30% ethanol/water to mention
Take, need to impregnate bunge pricklyash leaf 6 days in Extraction solvent every time, extracting times are 3 times, the bunge pricklyash leaf and ethanol/water it is mixed
The mass ratio for closing solution is 1:10, has extracted filtering, merging filtrate on a rotary evaporator heating water bath to 50 DEG C of vacuum decompressions
Concentration, it is dry, obtain general flavone crude extract A；
(3) Chinese prickly ash leaf flavonoids crude extract is sufficiently dissolved with water, crosses AB-8 large pore resin absorption column.Water, crude extract are used respectively
All be added columns in after, standing adsorption for 24 hours, later in order respectively with the pure water of 3.5 times of BV, 30% ethyl alcohol, 60% ethyl alcohol, 85%
Ethyl alcohol and ethanol elution, each section of eluent is concentrated into thick paste respectively, is transferred in beaker, labelled, spare, and each section is compiled respectively
Number are as follows: A(pure water), B(30% ethyl alcohol), C(60% ethyl alcohol), D(85% ethyl alcohol), E(ethyl alcohol).
C portion elutes general flavone volume parts >=40% of Chinese prickly ash extractive of general flavone in medicinal extract, and concentration highest as has
There is the Chinese prickly ash leaf total flavone extract of the drug of hypoglycemic effect, health food or food.
(4) it takes C portion elution 90 g of medicinal extract to be dissolved in 7200 g water, the sweetener of 7g mass concentration 8%, 7g mass is added
The sucrose ester of concentration 0.03%, the calcium lactate of 7g mass concentration 0.2%, the xanthan gum and 0.4g vitamin C of 7g mass concentration 3.0%,
B1、B2、P。
(5) deployed mixed solution is placed in homogenizer, is 15MPa in homogenization pressure, under the conditions of 45 DEG C of temperature
Matter makes each homogenization of composition, prevents from being layered, and guarantees the stability of product.
(6) mixed solution to homogenize is centrifuged 20 min in the centrifuge of 8000 r/min of room temperature, discards precipitating, receives
Collect supernatant.
(7) by filtered clear solution in 125 DEG C of progress 6 s of instantaneous sterilizing, be cooled to room temperature it is rear it is filling up at
Product.
Embodiment 3:
(1) 5.0 kg of bunge pricklyash leaf is cleaned, is dried in the shade, then crushed, smashing fineness is 4 mm；
(2) mixed solution for the ethanol/water for being 60% with percentage by volume by smashed bunge pricklyash leaf carries out leakage extraction, seeps
Leakage, which is extracted, to need to impregnate bunge pricklyash leaf into 6-24h in Extraction solvent, then is placed in leakage equipment, the mixing of bunge pricklyash leaf and ethanol/water
The mass ratio of solution is 1:10, is extracted 3 times, and extraction time is 2 h/ time, has extracted filtering, merging filtrate, in Rotary Evaporators
Upper heating water bath to 60 DEG C of vacuum-concentrcteds, drying obtains general flavone crude extract A；
(3) Chinese prickly ash leaf flavonoids crude extract is sufficiently dissolved with water, crosses D-101 large pore resin absorption column.It is mentioned respectively with water, slightly
Object all be added columns in after, standing adsorption for 24 hours, later in order respectively with the pure water of 3.5 times of BV, 30% ethyl alcohol, 60% ethyl alcohol,
85% ethyl alcohol and ethanol elution, each section of eluent is concentrated into thick paste respectively, is transferred in beaker, labelled, spare, and each section point
Do not number are as follows: A(pure water), B(30% ethyl alcohol), C(60% ethyl alcohol), D(85% ethyl alcohol), E(ethyl alcohol).
C portion elutes general flavone volume parts >=40% of Chinese prickly ash extractive of general flavone in medicinal extract, and concentration highest as has
There is the Chinese prickly ash leaf total flavone extract of the drug of hypoglycemic effect, health food or food.
(4) take C portion elution 90 g of medicinal extract be dissolved in 8100 g water, be separately added into the mass concentration 15% of 7g sweetener,
The xanthan gum and 0.3g of the sucrose ester of mass concentration 0.05%, the calcium lactate of mass concentration 0.15-0.2% and mass concentration 3.0%
Vitamin C, B1, B2, P.
(5) deployed mixed solution is placed in homogenizer, is 30 MPa in homogenization pressure, under the conditions of 60 DEG C of temperature
Matter makes each homogenization of composition, prevents from being layered, and guarantees the stability of product.
(6) mixed solution to homogenize is centrifuged 20 min in the centrifuge of 9000 r/min of room temperature, discards precipitating, receives
Collect supernatant.
(7) by filtered clear solution in 130 DEG C of progress 6 s of instantaneous sterilizing, be cooled to room temperature it is rear it is filling up at
Product.
Embodiment 4:
(1) 5.0 kg of bunge pricklyash leaf is cleaned, is dried in the shade, then crushed, smashing fineness is 5 mm；
(2) smashed bunge pricklyash leaf is subjected to ultrasonic extraction with ethyl alcohol, the Extracting temperature of ultrasonic extraction is 50 DEG C, and frequency is
The mass ratio of 40Hz, power 800W, the bunge pricklyash leaf and ethyl alcohol is 1:10, is extracted 3 times, and extraction time is 2 h/ time, extraction
Complete filtering, merging filtrate, heating water bath to 70 DEG C of vacuum-concentrcteds, dry on a rotary evaporator, obtains general flavone and slightly mentions
Take object A；
(3) Chinese prickly ash leaf flavonoids crude extract A is sufficiently dissolved with water, crosses HPD-100 large pore resin absorption column.Respectively with water,
Crude extract all be added columns in after, standing adsorption for 24 hours, later in order respectively with the pure water of 3.5 times of BV, 30% ethyl alcohol, 60% second
Alcohol, 85% ethyl alcohol and ethanol elution, each section of eluent is concentrated into thick paste respectively, is transferred in beaker, labelled, spare, and each section
Number respectively are as follows: A(pure water), B(30% ethyl alcohol), C(60% ethyl alcohol), D(85% ethyl alcohol), E(ethyl alcohol).
C portion elutes general flavone volume parts >=40% of Chinese prickly ash extractive of general flavone in medicinal extract, and concentration highest as has
There is the Chinese prickly ash leaf total flavone extract of the drug of hypoglycemic effect, health food or food.
(4) it takes C portion elution 90 g of medicinal extract to be dissolved in 9000 g water, is separately added into the sweet taste of the mass concentration 15% of 7g
Agent, the sucrose ester of mass concentration 0.03%, the calcium lactate of mass concentration 0.15% and mass concentration 1.0% xanthan gum and 0.3g
Vitamin C, B1, B2, P.
(5) deployed mixed solution is placed in homogenizer, is 20 MPa in homogenization pressure, under the conditions of 50 DEG C of temperature
Matter makes each homogenization of composition, prevents from being layered, and guarantees the stability of product.
(6) mixed solution to homogenize is centrifuged 20 min in the centrifuge of 10000 r/min of room temperature, discards precipitating,
Collect supernatant.
(7) by filtered clear solution in 125 DEG C of progress 6 s of instantaneous sterilizing, be cooled to room temperature it is rear it is filling up at
Product.
Claims (8)
1. the preparation method of extractive of general flavone with health care function in a kind of bunge pricklyash leaf, it is characterised in that the preparation method
The following steps are included:
(1) bunge pricklyash leaf is cleaned, is dried in the shade, then crushed, smashing fineness is 0.1-5 mm；
(2) by smashed bunge pricklyash leaf Extraction solvent by room temperature cold soaking, be heated to reflux, leak and one of ultrasonic extraction
Extracting method extracts, and the mass ratio of the bunge pricklyash leaf and Extraction solvent is 1:3-20, extracts 1-4 times, has extracted filtering, closes
And filtrate, heating water bath to 40-70 DEG C of vacuum-concentrcted, dry on a rotary evaporator, obtains general flavone crude extract A；
(3) Chinese prickly ash leaf flavonoids crude extract A is sufficiently dissolved with water, the general flavone crude extract A and water quality ratio are 1:
5-20, then dissolved general flavone crude extract A is added in large pore resin absorption column, 24 h of standing adsorption divides in order later
Not Yong volume multiple be 3.5 times of pure water, 30% ethyl alcohol, 60% ethyl alcohol, 85% ethyl alcohol and ethanol elution, each section of eluent difference is dense
It is reduced to elution medicinal extract paste, the elution medicinal extract is Chinese prickly ash leaf total flavone extract with health care function, is transferred to burning
Cup in, labelled spare, each section is numbered respectively are as follows: A(pure water), B(30% ethyl alcohol), C(60% ethyl alcohol), D(85% ethyl alcohol), E
(ethyl alcohol).
2. according to claim 1 in a kind of bunge pricklyash leaf extractive of general flavone with health care function preparation method, it is special
Sign is: the Extraction solvent is the mixed solution for the ethanol/water that percentage by volume is 0-100%.
3. the preparation method of extractive of general flavone with health care function in a kind of bunge pricklyash leaf according to claim 1 or claim 2,
Be characterized in that: in step (2), the room temperature cold soaking extraction needs in Extraction solvent to impregnate bunge pricklyash leaf 3-10 days every time,
The heating temperature of the heating and refluxing extraction is 50-100 DEG C, is extracted 0.5-3.0 hours every time, and the leakage, which is extracted, to be needed to spend
Green pepper leaf impregnates 6-24h in Extraction solvent, then is placed in leakage equipment, and the Extracting temperature of the ultrasonic extraction is 30-60 DEG C, frequency
For 20-50Hz, power 70-1000W, extract 0.5-2.0 hours every time.
4. according to claim 1 in a kind of bunge pricklyash leaf extractive of general flavone with health care function preparation method, it is special
Sign is: macroporous absorbent resin is HPD-600, X-5, AB-8, polyamide, HZ-841, D-101 and HPD- in the step (3)
One of 100 or a variety of.
5. the purposes of extractive of general flavone with health care function, feature in a kind of bunge pricklyash leaf according to claim 1
Be to include preparing health beverages, the method for preparing health beverages the following steps are included:
(1) ingredient: the elution medicinal extract of C portion obtained in the claims 1 is soluble in water, the mass ratio of the medicinal extract and water
For 1:50-200, it is dense to add sucrose ester, quality that sweetener, mass concentration that mass concentration is 5%-15% are 0.02-0.05%
It is raw to spend the calcium lactate for being 0.15-0.2%, the xanthan gum that mass concentration is 1.0-3.0% and the dimension that mass concentration is 0.01-0.03%
Plain C, B1, B2, P；
(2) mixed solution deployed in step (1) ingredient is placed in homogenizer, is 15-30 MPa, temperature in homogenization pressure
Homogeneous under the conditions of 45-60 DEG C makes each homogenization of composition, prevents from being layered, and guarantees the stability of product；
(3) filter: the mixed solution after homogenizing is centrifuged by the centrifuge of 6000-10000 r/min at room temperature
10-20 min discards precipitating, collects supernatant；
(4) it sterilizes filling: by filtered clear solution in 120-130 DEG C of progress instantaneous sterilizing 4-6 s, after being cooled to room temperature
It is filling to get product.
6. the purposes of extractive of general flavone with health care function, feature in a kind of bunge pricklyash leaf according to claim 5
Be: in the step (1), sweetener is the syrup of glucose or fructose, sucrose, maltose, starch sugar, lactose.
7. the purposes of extractive of general flavone with health care function, feature in a kind of bunge pricklyash leaf according to claim 5
Be: the health beverages of the preparation have enhancing and adjust the function of human immunity.
8. the purposes of extractive of general flavone with health care function, feature in a kind of bunge pricklyash leaf according to claim 5
Be: the health beverages of the preparation have anti-oxidant, anti-aging, cosmetology, eliminate fatigue and prevention cardiovascular and cerebrovascular disease
Function.
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| CN109875047A (en) * | 2019-04-04 | 2019-06-14 | 云南农业大学 | A kind of wheaten food auxiliary additive for alleviating anorectal disease |
| CN110169560A (en) * | 2019-06-19 | 2019-08-27 | 武汉轻工大学 | A kind of spice extract and spice extract liposome and preparation method and application |
| CN110327395A (en) * | 2019-07-22 | 2019-10-15 | 兰州大学 | Application of the Chinese prickly ash leaf total flavone extract in anti anoxia |
| CN111418745A (en) * | 2020-03-23 | 2020-07-17 | 西北农林科技大学 | Chinese prickly ash-Jinlumei leaf composite beverage and preparation method thereof |
| CN111418744A (en) * | 2020-03-23 | 2020-07-17 | 西北农林科技大学 | Chinese prickly ash leaf beverage and preparation method thereof |
| CN112516621A (en) * | 2020-11-16 | 2021-03-19 | 晨光生物科技集团股份有限公司 | Preparation method of high-content Chinese prickly ash flavone |
| CN112772767A (en) * | 2021-01-27 | 2021-05-11 | 四川省食品发酵工业研究设计院有限公司 | Method for extracting protein from pepper by-product |
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| CN111418744A (en) * | 2020-03-23 | 2020-07-17 | 西北农林科技大学 | Chinese prickly ash leaf beverage and preparation method thereof |
| CN112516621A (en) * | 2020-11-16 | 2021-03-19 | 晨光生物科技集团股份有限公司 | Preparation method of high-content Chinese prickly ash flavone |
| CN112772767A (en) * | 2021-01-27 | 2021-05-11 | 四川省食品发酵工业研究设计院有限公司 | Method for extracting protein from pepper by-product |
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